57 results about "Immunological Diagnosis" patented technology

The currently used method for immunological diagnosis of tuberculosis infection, the tuberculin skin test, is problematic for a number of reasons; it has low specificity in BCG vaccinated individuals, a high interobserver variance and requires skill to be read and interpreted. Furthermore it requires an extra visit to the clinic to have the test read. Both people vaccinated with BCG and those exposed to non-tuberculosis mycobacteria give a positive skin test result similar to that seen in a TB infected individual. This also applies for purified protein derivative (PPD) when used in a blood cell based test. The present invention discloses the development of an immunological TB diagnostic tool based on a combination of epitopes from proteins encoded by regions of the M. Tuberculosis (M. tub.) genome, that are not present in the BCG vaccine strain or in the most common non-tuberculosis mycobacteria. Four recently characterized proteins with this diagnostic potential were selected. Peptides from these proteins were tested one by one with peripheral blood mononuclear cells from microscopy or culture confirmed TB patients as well as from healthy BCG vaccinated controls. Some combinations of peptides showed a sensitivity level comparable to the level seen with the two wellknown M. tuberculosisspecific proteins ESAT 6 and CFP 10. An epitope combination with these peptides combined with ESAT 6 and CFP 10 gave a sensitivity of 93%, representing a raise in sensitivity of about 26-33% compared to using ESAT6 or CFP 10 alone. The results from a panel of TB patients, using a collection of the new specific epitopes clearly demonstrates, that addition of other specific epitopes to the already known specific antigens, increases the sensitivity of a diagnostic assay based on cell mediated immune response.

The currently used method for immunological diagnosis of tuberculosis infection, the tuberculin skin test, is problematic for a number of reasons; it has low specificity in BCG vaccinated individuals, a high interobserver variance and requires skill to be read and interpreted. Furthermore it requires an extra visit to the clinic to have the test read. Both people vaccinated with BCG and those exposed to non-tuberculosis mycobacteria give a positive skin test result similar to that seen in a TB infected individual. This also applies for purified protein derivative (PPD) when used in a blood cell based test. The present invention discloses the development of an immunological TB diagnostic tool based on a combination of epitopes from proteins encoded by regions of the M. tuberculosis (M. tub.) genome, that are not present in the BCG vaccine strain or in the most common non-tuberculosis mycobacteria.

The invention provides a nano magnetic bead labeled by chicken yolk immune globulin IgY and used for resisting the soluble antigen of schistosoma japonica ovums and a chicken yolk antibody-magnetic bead ELISA (Enzyme-Linked Immuno Sorbent Assay) method for detecting schistosoma japonica circulating antigen. The method comprises the steps of: immunizing a hen with specific antigens of schistosoma japonica with a method of subcutaneous multi-point injection, and extracting, purifying and identifying specific IgY from an egg yolk; and coupling the polyclone IgY on a magnetic bead, and detecting the circulating antigen of the schistosoma by using the magnetic bead-IgY polyclone antibody as a capture antibody and using the specific monoclone IgY antibody as a detecting antibody. The method not only can be used for capturing more antigens by using the magnetic bead and the IgY to increase the sensibility, but also benefits the increase of detecting specificity by using the monoclonal antibody. Thus, the high combination and coordination of sensibility and specificity, required in immunology diagnosis, are realized.

The invention discloses an epitope for sicca syndrome and application thereof, and belongs to the technical field of immunological diagnosis. The epitope is a polypeptide, and an amino acid sequence of the epitope is shown as SEQ ID NO: 1. The epitope is the polypeptide, and enzyme-linked immunosorbent assay (ELISA) detection and the reaction condition of the blood serum of patients indicate that the epitope can be combined with immunoglobulin G (IgG) in the blood serum of the patients specifically and is not reacted with healthy blood. The epitope polypeptide can be used for preparing medicines for diagnosing sicca syndrome.

The invention relates to an indirect ELISA kit for cat toxoplasma gondii serum antibody and a preparation method thereof. The toxoplasma gondii in serum is detected by adopting toxoplasma gondii surface protein SAG3 as a coating antigen; an indirect ELISA method is established by adopting HRP marked goat anti-cat IgG as a detection antibody, which has relatively strong specificity, sensitivity and repeatability; according to the invention, except for the fact that the washing liquid is powder, the main reagents can be directly applied after being dissolved in water; for the kit, the laboratory detection washing liquid is used as a basic solution, and the rest all exists in a form of liquid working solution, thus the kit is convenient to use; and the invention provides a convenient and reliable immunological diagnosis method for large sample detection and epidemiological survey and provides a basis for the diagnosis of cat toxoplasmosis.

The invention relates to a cell and protein combined analysis device and combined analysis method, and the device comprises a sample storage unit for carrying one or more whole-blood samples; an automatic sample feeding unit for sample loading, sample transferring and reagent addition operation; a cell analysis unit for classifying and counting cells of the sample; a protein analysis unit for protein biochemical and immunological detection of the sample; and a data processing unit for controlling automatic sample feeding operation, real-time collection of analysis results of the cell analysis unit and the protein analysis unit and display of processed results. Cell analysis and protein analysis are integrated in one device, automatic cell and protein combined analysis can be realized, and the cell and protein combined analysis device and combined analysis method have the advantages of high flux and simple operation, and can meet the clinical needs of simultaneous cell diagnosis and biochemical and immunological diagnosis of the blood samples.

The invention provides a transgenic animal having within its genome a transgene construct for gastrointestinal tract specific expression of a protein. In a preferred embodiment, the protein is a phytase or a homologue thereof. Such proteins may be heterologous and may be specifically expressed in the salivary gland of the animal by operably linking the nucleic acid sequence encoding the protein with regulatory sequence including a salivary gland protein promoter / enhancer. Also provided are methods of expressing and producing proteins using such nucleic acid constructs. Further, antibodies specific to such proteins and immunological diagnostic kits are also provided.