8 results about "Soluble antigen" patented technology

A nanoparticle and a preparation method therefor, the nanoparticle including an amphiphilic peptide, which forms a micelle structure through self-assembly, and a target peptide (preferably, a water-soluble antigen peptide), which electrically binds to the surface of the amphiphilic peptide. The target peptide electrically binds to the surface of the amphiphilic peptide micelle structure and becomes particulated, and thus can be effectively presented to an antigen-presenting cell, and the weight ratio of the amphiphilic peptide and the target peptide is controlled so that the size of nanoparticles is controlled and endocytosis thereof is carried out, and thus immunity by means of cytotoxic T cells can be induced. Nanoparticles exhibit use only an epitope of a more accurate region so as to be effective as a vaccine, and thus have minimal side effects. Therefore, excellent antigen-specific antibody and cell immunotherapy effects are exhibited, and thus can be used in various fields such as vaccine production.

The present application relates to the technical field of medicine, in particular to a functional nanomaterial based on choline phosphate-cell membrane interaction and application thereof.The present application modifies manganese mineralized black phosphorus nanomaterial by bio-inspired polymer pGluCP adsorption, which retains the excellent biocompatibility and photothermal performance of BP, and at the same time, pGluCP enhances the 'chassis' effect of the material, captures tumor-related antigens, and can simultaneously capture water-soluble antigens and water-insoluble membrane antigens and form pathogen-like micro-nanoparticles, which is helpful for antigen delivery and APC presentation, and provides a feasible tool for capturing non-water-soluble membrane antigens, which is a major challenge.MnBP and pGluCP synergistically act, and can realize the personalized conversion of autologous tumors to vaccine production and delivery.

The invention provides a monoclonal antibody for specifically recognizing cysts and oocysts / sporangiums of sheep sarcocysts and application of the monoclonal antibody, and the monoclonal antibody provided by the invention can generate positive reaction aiming at soluble antigens of adult holoproteins of sarcocysts tenella and soluble antigens of oocysts / sporangiums of the sarcocysts tenella. The two key development stages of adults and oocysts / sporangiums are covered at the same time. A complete parasite life cycle detection system can be established, the clinical sample detection rate is remarkably improved (experiment shows that the clinical sample detection rate is improved by 35-40%), and the detection window period is 7-10 days earlier than that of a traditional method. Meanwhile, the ELISA detection kit and the detection method thereof provided by the invention are high in sensitivity and convenient to operate, can greatly improve the detection efficiency, and have a good industrial application prospect.

The invention discloses a tumor holoantigen nano vaccine as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The nano vaccine comprises a composite liposome, a nucleic acid TLR agonist adjuvant, a tumor cell membrane antigen and a tumor cell soluble antigen. The membrane antigen is embedded into the lipid bilayer, and the soluble antigen and the adjuvant are wrapped in the inner cavity of the lipid to form the nanoparticles with accurate structures. The preparation method comprises the following steps: splitting a tumor cell separation membrane antigen and a soluble antigen; mixing the membrane antigen and lipid to form a lipid phase, and mixing the soluble antigen and an adjuvant to form a water phase; finally, the two phases are self-assembled into the nano vaccine through a micro-fluidic chip. The two antigens are synergistically combined at the nanoscale, the problems that a single antigen is weak in immunogenicity and incomplete in coverage are solved, the synergic anti-tumor effect far better than that of a single antigen vaccine is shown in an animal model, the preparation process is universal and controllable, and an efficient platform is provided for individualized tumor immunotherapy.

The invention discloses a method for preparing O-type foot-and-mouth disease virus multi-epitope virus-like particles through escherichia coli as well as a product and application thereof. The method comprises the following steps: inserting nucleic acid for coding O-type foot-and-mouth disease virus multi-epitope virus-like particles into a prokaryotic expression vector, then converting the prokaryotic expression vector into escherichia coli competent, and carrying out protein expression and purification, wherein the sequence of the nucleic acid for coding the O-type foot and mouth disease virus multi-epitope virus-like particle is as shown in SEQ ID NO. 5. The O-type foot and mouth disease virus multi-epitope VLP antigen is designed by taking HBc protein as a skeleton and all topological representative strain antigen epitopes of O-type foot and mouth disease virus separated in China as elements and introducing T cell epitopes, the antigen spectrum is wider, auxiliary T cell epitopes are introduced, rapid immune response can be excited in an early stage, and the recombinant antigen is soluble in expression, so that the recombinant antigen can be used for preparing the O-type foot and mouth disease virus multi-epitope VLP antigen for the O-type foot and mouth disease virus. And only the soluble antigen needs to be purified, so that the purification step is simplified, the antigen loss is reduced, and industrial production can be realized.

The invention provides an echinococcus granulosus antibody, a kit for detecting echinococcus granulosus pathogen from excrement and application of the echinococcus granulosus antibody. Firstly, a monoclonal antibody 4E6 is provided, the monoclonal antibody 4E6 can stably and efficiently recognize soluble antigens of adult echinococcus granulosus, soluble antigens excreted and secreted by the echinococcus granulosus and soluble antigens of eggs of the echinococcus granulosus, and the monoclonal antibody 4E6 has high titer and good specificity on the various mixed antigens. Therefore, the antibody can be used for preparing a kit for detecting echinococcus granulosus eggs in canine animal excrement or environment. The preparation method of the monoclonal antibody 4E6 for resisting echinococcus granulosus provided by the invention is simple, the antibody purification process is simple, the efficiency is high, and the cost is low. The monoclonal antibody 4E6 provided by the invention is used for detecting polluted echinococcus granulosus in excrement of canine animals or environment, and has the advantages of rapidness, sensitivity, high specificity, simplicity in operation, low cost and the like.

The invention discloses an antigen and adjuvant co-delivery nanocapsule vaccine and a preparation method thereof. The preparation method comprises the following steps: dissolving the polycaprolactone, the polycaprolactone-polyetherimide block copolymer, the adjuvant oil phase and the fat-soluble immune activator in ethanol to obtain a mixed solution; and carrying out solvent exchange on the obtained mixed solution and an isotonic solution in which the antigen is dissolved so as to obtain the antigen and adjuvant co-delivered nanocapsule vaccine. The nanocapsule vaccine is simple and efficient in preparation scheme, adopts a biocompatible material and a green solvent, is green and free of toxic and side effects, and can be suitable for large-scale vaccine production process popularization. The nanocapsule vaccine can realize co-delivery of the oil phase adjuvant, the water-soluble antigen and the water-soluble antigen, improves the immune response effect, and has a good prospect in the field of vaccine research and development.

The application provides a monoclonal antibody specifically recognizing a cyst and an egg / sporocyst of a sheep trichophyton and application thereof, the provided monoclonal antibody can positively react to a soluble antigen of a whole protein of a trichophyton adult and a soluble antigen of an egg / sporocyst, and simultaneously covers two key development stages of the adult and the egg / sporocyst. A complete parasite life cycle detection system can be established, and a clinical sample detection rate is significantly improved (experiments show that the detection rate is improved by 35-40%). Moreover, a detection window period is 7-10 days earlier than that of a traditional method. Meanwhile, the application provides an ELISA detection kit and a detection method thereof, the ELISA detection kit and the detection method thereof are high in sensitivity, convenient to operate, can greatly improve detection efficiency, and have a good industrial application prospect.