Liver fluke rapid immune detection kit for fresh water fishes and detection method

Abstract

The invention discloses a fresh water fish liver fluke fast immunity test kit and a test method. The kit box comprises an enzyme linked reaction board, an enzyme conjugate, washing liquid, a substrate solution, a color developer, a diluent and an end solution, wherei the enzyme linked reaction board is made by extracting and purifying soluble antigen from liver fluke and enveloping the soluble antigen; the enzyme conjugate is made by labeling anti-fish immunoglobulin via horseradish peroxidase. The invention utilizes the sensitivity, the specificity, the practicality and the economy of the fast immunity test technique to establish a fresh water fish liver fluke fast immunity test method, is practical and simple while the kit can be opened and used instantly, without preparation or dilution, with simple operation; the result can be judged quantitatively by instruments and can be judged by eyes, thus is suitable of the on-site detection of different conditions; the invention is safe and environment friend, which adopts the harmless enzyme linked immunity color developer as tetramethylbenzidine to avoid the harm and pollution of general methods.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a rapid immunological detection kit and detection method for freshwater fish liver fluke. Background technique [0002] Liver fluke is a common human parasite that is acquired by ingesting freshwater fish that contain liver fluke metacercariae. Liver fluke adults parasitize in the bile ducts of the human liver, leading to liver fluke disease, causing a series of liver and gallbladder damage, including bile duct inflammation, stones, biliary cirrhosis, and finally can induce liver cancer. [0003] Currently, there are many methods for diagnosing human liver fluke disease, including pathogenic methods to detect fecal eggs and immunological methods to detect liver fluke antibodies in patients' serum. The etiology method is accurate and reliable, but the eggs are small, ovulation is irregular, and it is easy to miss the diagnosis. In addition, it is necessary to collect...

AI Technical Summary

Problems solved by technology

The traditional fish liver fluke inspection is to use a microscope to observe the liver fluke metacercariae in the fish meat, but it is very difficult to observe the metacercariae less than 1 mm in the fish meat with a weight of 1000 grams in this method. , not only inefficient, but also prone to omission

Therefore, microscopic examination of fish meat and detection of liver fluke, except for scientific research, has hardly been applied in practice, which is also an important reason for the lack of safety supervision of freshwater fish liver fluke

However, the immunological detection method of freshwater fish liver fluke has not been established so far.

[0006] Therefore, the prior art does not have an effective tool for the detection of liver flukes in freshwater fish

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