20 results about "Fish liver" patented technology

The invention discloses a fish liver acellular matrix-based microfluidic 3D printing hydrogel for liver regeneration and a preparation method thereof, the fish liver acellular matrix (dECM) and gelatin methacryloyl (GelMA) are combined together, and induced pluripotent stem cell-derived hepatocytes (iPSC-hep) are loaded for liver regeneration. The microfluidic 3D printing hydrogel based on the fish liver acellular matrix has excellent biocompatibility, retains complete endogenous growth factors, retains the biological activity of cells, ensures effective cell encapsulation, and is beneficial to stable functional expression of iPSC-hep. After in-vivo transplantation, the survival rate and the liver function of acute hepatic failure mice are remarkably improved, and regeneration and repair of the liver are promoted.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention relates to an efficient cod liver oil separation and purification integrated device and process, and relates to the technical field of cod liver oil purification. Comprising a bottom plate, a treatment tank, a distillation tank and a purification unit are sequentially arranged on the bottom plate in the length direction of the bottom plate, and the purification unit comprises a reaction tank and a filtering tank which are arranged from top to bottom in the height direction of the bottom plate. According to the present invention, the following problems in the cod liver oil purification process in the prior art can be solved: by arranging the treatment tank, the distillation tank and the purification unit which are communicated, the fish liver is subjected to distillation extraction and low-temperature purification after pretreatment, such that the sealing state is ensured in the whole process, the oxidation due to the contact with the air is prevented, and the purification effect of the cod liver oil is improved; by combining a distillation extraction method and a low-temperature crystallization method, the cod liver oil can be effectively extracted, saturated fatty acid and part of monounsaturated fatty acid can be separated, liquid polyunsaturated fatty acid is reserved, and the method is suitable for wide health requirements.

A microfluidic 3D-printed hydrogel based on fish liver decellularized extracellular matrix for liver regeneration and a preparation method. The hydrogel is prepared by means of combining fish liver decellularized extracellular matrix (dECM) and gelatin methacryloyl (GelMA), and loading induced pluripotent stem cell-derived hepatocytes (iPSC-heps) for liver regeneration. The microfluidic 3D-printed hydrogel based on fish liver decellularized extracellular matrix exhibits excellent biocompatibility, retains intact endogenous growth factors, maintains the biological activity of cells, ensures the effective encapsulation of the cells, and facilitates the robust functional expression of the iPSC-heps. After in-vivo transplantation, the survival rate and liver function of mice with acute liver failure are significantly improved, and liver regeneration and repair are promoted.

The invention discloses a scatophagus argus liver fat storage cell separation and culture method, and belongs to the technical field of cell biology. According to the present invention, the scatophagus argus liver fat storage cells are obtained through separation by using a perfusion method, and then the scatophagus argus liver fat storage cells obtained through separation are cultured by using a DMEM medium sugar culture medium containing 8-12% of FBS, 4-6% of HBS, 0.005-0.015% of HGF, 0.005-0.015% of HGP, 0.12-0.16 nM of vitamin A acid and 2-2.5 mM of L-glutamine; the ITO cells obtained through separation can be normally cultured to five generations, and the cells have good cell viability.

A fish liver decellularized extracellular matrix based microfluidic 3D printing hydrogel for liver regeneration, and a preparation method thereof are provided. A fish liver decellularized extracellular matrix (dECM) is combined with gelatin methacryloyl (GelMA), and loaded with hepatic spheroids derived from induced pluripotent stem cells (iPSC-hep) for liver regeneration. The fish liver decellularized extracellular matrix based microfluidic 3D printing hydrogel of the present disclosure has excellent biocompatibility and retains intact endogenous growth factors, maintains the biological activity of cells, ensures effective cell encapsulation, and is conducive to robust functional expression of iPSC-hep. After being transplanted in vivo, the hydrogel significantly improves the survival rate and liver function of mice with acute liver failure, and promotes liver regeneration and repair.

The application belongs to the technical field of biomedicine, and discloses a method for constructing a Channa argus bile stasis model. The application discloses application of rifampicin or a pharmaceutically acceptable salt thereof in construction of a fish liver injury model. The application discloses, for the first time, that the antibiotic rifampicin widely used in aquaculture is used to construct a fish liver injury model. The model is closer to the real scene of the use of a large amount of drugs when bacterial diseases break out in production practice. The injury type of the fish liver injury model is clearly directed to bile stasis, which fills the research blank of the disease model of the fish, and is different from common hepatocyte necrosis or fatty liver models.

The invention relates to a method for extracting, purifying and identifying high-purity fish liver surrounding lipid droplet mitochondria, and belongs to the technical field of animal biology.The method comprises the following steps that S1, fish gill cutting, bloodletting, liver taking, cleaning and impurity removal are conducted, the washed liver is cut into pieces, grinding is conducted, and then unbroken cells and tissue blocks are removed through centrifugation; and S2, performing low-speed centrifugation to separate the fat layer, and then performing high-speed centrifugation to separate mitochondria from the fat layer. According to the method for extracting, purifying and identifying the high-purity fish liver lipid droplet mitochondria, the purification and identification method is used for extracting the fish liver lipid droplet mitochondria, the operation is simple and easy to implement, the extraction efficiency is high, and PDM impurities extracted by utilizing tissues are few; according to the present invention, the cell debris problem existing in the existing method is completely removed by performing multiple differential centrifugation, the cytoplasm pollution is reduced by using multiple resuspension steps in the differential centrifugation, and the contained impurities are diluted by continuously expanding the resuspension ratio so as to improve the purity.

This application discloses a combination of gut microbial biomarkers, a system, and its application for detecting liver injury in fish, belonging to the field of molecular detection technology for fish liver injury. The gut microbial biomarker combination includes *Cetobacter* spp. (…). Cetobacterium ), Pseudomonas spp. Pseudomonas ) and Escherichia coli-Shigella spp. Escherichia‑Shigella Using the intestinal microbial biomarker combination described in this application, non-invasive diagnosis of liver damage can be achieved without touching the fish body, with high accuracy and no missed detections, especially avoiding misdiagnosis as liver damage that could lead to overtreatment. This provides a basis for judgment in aquaculture environment control and precise disease prevention and control, and is beneficial for improving the survival rate and profitability of yellow catfish farming.

The invention discloses an intestinal microbial marker combination and system for detecting fish liver injury and application, and belongs to the technical field of molecular detection of fish liver injury. The invention relates to an enteric microbial marker combination, which is characterized in that the enteric microbial marker combination comprises the following components: cetyl, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella. By utilizing the intestinal microbial marker combination, non-invasive diagnosis of liver injury can be realized on the premise of not invading a fish body, the accuracy is high, missing detection is avoided, and particularly, the condition that liver injury is misjudged to cause excessive treatment is avoided. A judgment basis is provided for breeding environment regulation and control and precise disease prevention and treatment, the breeding survival rate of the pelteobagrus fulvidraco is increased, and the breeding benefit is improved.

The invention belongs to the technical field of fish feed, and particularly relates to functional feed for promoting hepatopancreas of leuciscus waleckii to be healthy and application. The functional feed for promoting hepatopancreas health of the leuciscus waleckii comprises a basal feed and an additive chlorogenic acid, the addition amount of the chlorogenic acid is 0.02 to 0.08 percent. The functional feed capable of promoting hepatopancreas health of the leuciscus waleckii is simple in component, easy in raw material obtaining, safe and harmless, and by adding a proper amount of chlorogenic acid, the fish growth and feed utilization rate can be effectively improved, the lipid metabolism function, the immune function and the oxidation resistance are improved, and the growth performance and hepatopancreas lipolysis of the leuciscus waleckii are effectively promoted.

The application discloses an extraction method of sturgeon oil with skin repair function, which comprises the following steps: S1, preparation of crude fish oil of fish fat; S2, preparation of crude fish oil of fish liver; S3, preparation of crude fish oil of fish skin; S4, degumming and deacidification; and S5, refining. The fish oil extracted from different parts of sturgeon is mixed according to a specific proportion, the fish fat fish oil, the fish liver oil and the fish skin oil are mixed, and three kinds of functional components are added in the extraction process respectively, i.e. dihydroquercetin (providing antioxidant protection), phosphatidylserine (enhancing cell membrane repair and nutrient absorption) and curcumin (bringing anti-inflammatory and whitening effects), so that a multiple synergistic formula system is formed, and the prepared sturgeon oil is significantly enhanced in the repair function on the skin, and the bioavailability and stability of the product are improved.

The application discloses application of Prunella vulgaris extract in prevention and treatment of fish parasitic disease infection. Inclined tube worm in-vitro soaking experiments show that the insecticidal effect of Prunella vulgaris extract and zinc sulfate is equivalent after 48 hours, and the mortality rate is above 95.44%; based on the growth condition of rainbow trout feeding on Prunella vulgaris extract, the specific growth rate of fish is significantly improved, the growth performance is improved, and the growth of fish is promoted; the plasma biochemical indexes after parasitic infection show that the addition of 0.25% to 1% Prunella vulgaris extract in feed can significantly reduce stress caused by pathogenic stress and protect the health of fish liver.

This invention discloses a pulsed matrix electric field device and its application in killing liver flukes in fish. The device includes an insulating outer shell and an insulating cover movably mounted on the insulating outer shell. Inside the insulating cover is a matrix of stainless steel spring contacts connected to the positive terminal of a power supply. Inside the insulating outer shell is a tray support for holding a stainless steel tray containing sashimi. After the stainless steel tray is placed, it is connected to the negative terminal of the power supply via the tray support. When the insulating cover is fastened onto the insulating outer shell, the stainless steel spring contacts in the stainless steel spring contact matrix make elastic contact with the sashimi. A high-voltage pulsed current is applied to the sashimi by the power supply to kill the liver flukes. This invention can efficiently kill metacercariae in fish meat while maintaining the quality of the fish, laying a solid foundation for the control of liver flukes and having significant implications for the control of Clonorchiasis sinensis. It can also be applied to the control of parasites in other meat products.

The invention discloses a miRNA inhibitor for improving fish insulin resistance, a culture method and application of the miRNA inhibitor, the miRNA inhibitor is a miR-29a inhibitor, the nucleotide sequence of the miR-29a inhibitor is shown as SEQ ID NO: 3, and the miR-29a inhibitor improves the pik3r1 gene expression level by inhibiting miR-29a expression. Through targeted intervention of miR-29a and auxiliary feeding of the high-sugar feed containing balsam pear saponin, fish liver insulin resistance induced by the high-sugar feed is relieved, and the high-sugar feed utilization capacity of carps is improved.

The invention discloses an extraction method of sturgeon oil with a skin repairing function. The extraction method comprises the following steps: S1, preparing crude fish oil of fish fat; s2, preparing fish liver crude fish oil; s3, preparing fish skin crude fish oil; s4, degumming and deacidifying; and S5, refining. Fish oil extracted from different parts of sturgeons is mixed according to a specific proportion, and the fish fat oil, the cod liver oil and the fish skin oil are mixed according to a specific proportion. Meanwhile, three functional components, namely dihydroquercetin (providing antioxidant protection), phosphatidylserine (enhancing cell membrane repair and nutrient absorption) and curcumin (bringing anti-inflammatory and whitening effects), are respectively added in the extraction process, so that a multi-synergistic formula system is formed; according to the prepared sturgeon oil, the skin repairing function is remarkably enhanced, and meanwhile the bioavailability and stability of the product are improved.

The present invention provides a liver protection composition prepared from a plant extract and a corn-derived polypeptide, wherein the polypeptide is prepared by enzymolyzing corn residue with alkaline protease and ficin, and centrifuging, decolorizing and drying the enzymolysis product; the enzymatic activity ratio of the alkaline protease and ficin is 2:1; the molecular weight of the enzymolysis product is 500-3000Da; the amino acid sequence of one polypeptide fragment of the polypeptide is KDYASKNCLQSFMLM. The polypeptide provided by the present invention has the effect of protecting the liver of fish, and when used in combination with the plant extract, it can achieve a high concentration effect with a smaller dosage.

The invention relates to the technical field of fish detection, in particular to an automatic evaluation method for a fish liver fat deposition state based on image segmentation and color feature analysis, and the method comprises the following steps: obtaining an image; segmenting organs; feature extraction: extracting an organ image based on the segmentation mask of the organ region, converting the organ image into an HSV color space, calculating color moment features of each channel of H, S and V, the color moment at least including a first moment, a second moment and a third moment; and fat deposition determination: inputting the color moment characteristics into a classification model to obtain a fat deposition state evaluation result of the liver, and the method can automatically identify a target organ region in an anatomical image and perform objective and quantitative evaluation on the fat deposition state of the liver based on the color characteristics with clear physical significance. By constructing an image processing flow consisting of organ segmentation, color feature extraction and classification judgment, the method can realize efficient, standardized and interpretable automatic evaluation of the fish liver fat deposition state.

The invention discloses a compound feed additive for relieving high-fat-induced liver injury of micropterus salmoides as well as a preparation method and application of the compound feed additive, the additive is prepared from guar gum, sodium butyrate and naringenin, and the mass percent of the guar gum, the sodium butyrate and the naringenin in a feed is 0.05-0.2%, and the mass percent of the sodium butyrate and the mass percent of the naringenin are 0.05-2%. It is found for the first time that the guar gum-sodium butyrate-naringenin combination with a specific ratio can cooperatively relieve fish liver injury through multiple channels, reduce liver lipid deposition of micropterus salmoides, enhance liver oxidation resistance, apoptosis resistance and inflammation resistance, and comprehensively improve the fish liver health level.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus reuteri YZ-1 and application thereof. The lactobacillus reuteri YZ-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34974, and the lactobacillus reuteri YZ-1 is separated from the intestinal tract of an aquatic animal zebra fish. The strain can relieve fish liver and intestine injury induced by high-iron feed and reduce the content of total iron and ferrous iron in the liver; the fermentation supernate inhibits streptococcus dysgalactiae, but thalli do not inhibit streptococcus dysgalactiae; the survival rate of the zebrafish infected by the edwardsiella tarda is improved; expression of inflammatory factors is inhibited, and intestinal inflammation is improved. The invention also provides a microbial inoculum containing the strain and a functional fish feed. The strain disclosed by the invention has the functions of relieving iron overload, resisting infection and resisting inflammation, is unique in antibacterial mode and is suitable for being applied to aquaculture.