31 results about "Intestine injury" patented technology

The invention discloses lactobacillus fermentum ZS40, which has a prevention effect on colitis, and the preservation number of the lactobacillus fermentum ZS40 is CGMCC NO.18226. The strain has high gastric acid resistance, and the survival rate of the strain treated by artificial gastric juice with the pH value of 3.0 for 3 h reaches 79.32%; and in 0.30% cholate, the growth efficiency of the strain reaches 15.31% of that of cholate-free culture. The research finds that the strain not only can inhibit mouse colon shortening, colon injury, intestinal wall thickening and other phenomena, but also can increase T-SOD and CAT activity, reduce MPO and MDA contents, regulate balance imbalance of pro-inflammatory cytokines and anti-inflammatory cytokines, and inhibit activation of NF-kappa B and MAPK signal channels so as to better relieve inflammation. In conclusion, the strain can effectively relieve mouse symptoms of colitis and can be used for preventing and treating colitis.

The invention belongs to the technical field of biomedicine, particularly relates to the application of propolis flavonoids in curing acute radioactive intestine injuries, and is used for curing the injuries of abdominal intestine tubes caused by radioactive rays after abdominal tumor radiotherapy. According to the application of propolis flavonoids in curing the acute radioactive intestine injuries, by accepting the radiotherapy and taking propolis flavonoids propolis orally, the DAO and NO expressions of the injured intestines are suppressed effectively, so that the propolis flavonoids is proved to be effective to suppress the injuries of intestinal epithelial cells prominently and lower the permeability of intestinal mucosa in a microscopic view, as a result, the intestinal mucosa shielding function is protected effectively, the intestinal bacterial translocation infection is prevented, and the pathological injury of the intestinal mucosa is alleviated prominently, besides, the propolis flavonoids can repair and protect the radioactive injuries of the intestinal mucosa.

The invention discloses lactobacillus rhamnosus FLRH93. The lactobacillus rhamnosus FLRH93 is preserved in the Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No:60956. Animal damage intervention experiment results show that the lactobacillus rhamnosus FLRH93 can obviously improve the intestinal injury caused by drugs; tissue staining sections show that the strain maintains the integrity of a small intestine structure, reduces the loss of goblet cells after drug treatment, and can regulate and control the secretion of Bcl-2 and NLRP3, thereby reducing generation of inflammatory factors IL1-beta and TNF-alpha. The invention belongs to the technical field of microorganisms. The strain provided by the invention can improve the survival rate of mice withdrug injury and can also remarkably promote healing of wound skin.

The invention discloses a traditional Chinese medicine composition. The composition is prepared from, by weight, 200-205 parts of radix rehmanniae preparata, 130-140 parts of Chinese pyrola herbs, 130-140 parts of rhzizoma drynariae, 130-140 parts of suberect spatholobus stem, 130-140 parts of cistanche deserticola, 130-140 parts of epimedium herbs and 80-90 parts of fried radish seeds. The invention further discloses a preparation method and application of the traditional Chinese medicine composition. The composition has the functions of tonifying the kidney, activating blood, diminishing inflammation and relieving the pain. The traditional Chinese medicine composition is used for hypertrophic myelitis, cervical spondylosis, calcaneal spur, proliferative arthritis and Kashin-Beck diseases. The composition further has the functions of improving mesentery circulation and promoting functional repairing of the stomach and intestines and is applied to treatment of stomach and intestine injuries and the like.

The invention discloses a mixed feed additive for eel capable of increasing the proportion of brown fish meal in a feed, a preparation method and application thereof. Each kilogram of the mixed feed additive for eel contains 80-250g of procyanidine, 20-100g of macleaya cordata powder, 150-800g of tributyrin, 50-250g of bile acid and the balance of a carrier. The addition amount of the mixed feed additive for eels in a feed is 0.2%-0.6%, the use amount of brown fish meal can be obviously increased, liver and intestine injuries caused by toxic and harmful substances in the brown fish meal are relieved, the feed efficiency is improved, and growth is promoted. The mixed feed additive has the characteristics of easily available raw materials, moderate cost, safety, effectiveness, no incompatibility, simple preparation, low addition amount and the like, and has a good application prospect.

Disclosed are methods of assessing the ability of a candidate therapeutic agent to reverse, reduce or prevent intestinal injury by a potential toxic agent using a three-dimensional, engineered, bioprinted, biological intestinal tissue model. Also disclosed are methods of assessing the effect of an agent on intestinal function, the method comprising contacting the agent with a three-dimensional, engineered, bioprinted, biological intestinal tissue model.

The invention belongs to the field of radiation protective agents, and in particular relates to new application of sanguinarine to the preparation of an ionizing radiation protective agent. The sanguinarine serving as the ionizing radiation protective agent can prolong the survival days of mice injured by radiation obviously, improve the survival rate of the mice obviously and reduce intestine injury, liver injury and pneumorrhagia of the mice injured by radiation obviously. Therefore, in actual application, practitioners who are engaged in radioactive work, patients who receive radiotherapy or other personnel who suffer from radioactive injury are subjected to the pretreatment of the sanguinarine before being exposed to ionizing radiation, the occupation exposure of radiation injury can be reduced, toxic and side effects caused by the radiotherapy can be relieved, and biohazards and the like caused by nuclear leakage can be prevented.

The invention provides a new medical application of a chiral spiro indole-pyran pyrimidine base compound, particularly application of the compound in preparation of anti-inflammatory drugs. An in vitro cyclooxygenase (COX) activity inhibiting experiment, an experiment of inhibiting generation of an inflammatory molecule (nitric oxide) in primary mice macrophage and inhibition test on an ear swelling and paw edema model in mice, show that the chiral compound has an obvious anti-inflammatory effect and has extremely low gastrointestinal injury effect; therefore, the chiral compound, as an active ingredient, can be prepared into anti-inflammatory drugs in various dosage forms through pharmaceutically acceptable technologies and auxiliary materials, so that the chiral compound has an excellent medical application prospect.

An object of the present invention is to provide an agent for preventing or treating small intestinal injury induced by a non-steroidal anti-inflammatory drug and a proton pump inhibitor. The present invention provides an agent for preventing or treating small intestinal injury induced by a non-steroidal anti-inflammatory drug and a proton pump inhibitor, and an agent for preventing or treating a fat-associated disease and / or inflammation accompanying the small intestinal injury, the agents comprising Bifidobacterium bifidum, Bifidobacterium longum or Bifidobacterium infantis or a processed product thereof.

The invention belongs to the field of medical technology, and relates to application of valeric acid in the preparation of medicine for preventing and / or curing tumor radiotherapy side reactions. Researches find that valeric acid can obviously improve intestinal injury, caused by partial irradiation on abdomen of gamma ray, of a mouse, so that the survival rate of the laboratory mouse is obviouslyincreased, the weight is expected to be increased, the level of intestinal inflammation is obviously reduced; and no paradoxical reaction appears on the laboratory mouse, so that valeric acid can beeffectively used for preventing and / or curing tumor radiotherapy side reactions, and the toxic and / or side effects are relatively low.

The invention discloses a lactobacillus rhamnosus (Lactobacillus rhamnosus FLRH93), which is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 60956. The results of injury intervention experiments in animals showed that Lactobacillus rhamnosus FLRH93 can significantly improve the intestinal injury caused by drugs. Histological stained sections showed that the strain maintained the structural integrity of the small intestine, reduced the loss of goblet cells after drug treatment, and It can regulate the secretion of Bcl‑2 and NLRP3, thereby reducing the production of inflammatory factors IL1‑β and TNF‑α. The invention belongs to the technical field of microbes, and the bacterial strain provided can improve the survival rate of drug-injured mice, and can also significantly promote the healing of wounded skin.

The invention provides application of costus lactone and a derivative thereof in preparation of a medicine for preventing and / or treating intestinal injury, and belongs to the technical field of chemical medicines. Specifically, the invention provides application of a compound shown as a formula I, or a salt thereof, or a stereoisomer thereof, or a solvate thereof, or a hydrate thereof, or a prodrug thereof in preparation of a medicine for preventing and / or treating intestinal injury, and R1 and R2 are respectively and independently selected from C1-C8 alkyl groups. Research finds that costus lactone has a protective effect on intestinal epithelial cell injury, especially radiation-induced intestinal epithelial cell injury, can effectively prevent and / or treat intestinal injury, especially radiation intestinal injury, especially radiation enteritis, can prolong the survival rate and improve the life quality of a patient, and has good safety within an effective dose. The costus lactone can be used for preparing medicines for preventing and / or treating intestinal injury, especially radioactive intestinal injury, and has a good application prospect.

The invention discloses a preparation for preventing and treating black body color missing symptoms of pelteobagrus fulvidracos due to poor feed freshness. The preparation is prepared from the following methods that the traditional Chinese medicine raw materials including herba ecliptae, ficus carica, cortex moutan, flastem milkvetch seeds and fructus cnidii are used; A, medicine juice decoction is performed: the herba ecliptae, the ficus carica, the cortex moutan, the flastem milkvetch seeds and the fructus cnidii are added into water for decoction; medicine juice is filtered and taken; then, chlorpheniramine maleate is added into the medicine juice; the medicine juice is concentrated to obtain a mixed solution A; B, coating is performed: the mixed solution A is coated to obtain the preparation for preventing and treating black body color missing symptoms of the pelteobagrus fulvidracos due to poor feed freshness. The preparation can be used for effectively preventing and treating the black body color missing occurring symptoms of the pelteobagrus fulvidracos due to fish meal staleness in feed, and also achieves a better repairing effect on stomach and intestine injury due to fish meal staleness. Therefore wide application prospects are realized.

The invention belongs to the technical field of radiation medicine, and specifically relates to a method for building a mouse acute radiation duodenitis model. According to the method, 8Gy gamma-ray irradiation is performed on mice, the irradiation part is the abdomen, duodenitis pathological analysis is performed on the mice after two weeks since the irradiation is conducted, and the protein expression levels of an apoptosis related factor BCL-2 and an inflammation related factor TGF-beta1 are detected. According to the invention, an irradiation method which is closer to clinical radiotherapyis adopted to build the radiation duodenitis model, thereby providing an experimental basis for prevention and treatment research for clinical radiation intestinal injuries.

The invention discloses a lactobacillus reuteri for relieving gastrointestinal injury caused by capsaicin and application thereof, and belongs to the technical field of microorganisms. The invention provides a lactobacillus reuteri CCFM1175 as well as a composition, a fermented food, application and a preparation method of a microbial inoculum of the lactobacillus reuteri CCFM1175. The lactobacillus reuteri CCFM1175 can significantly improve the mental state of mice after gavage of capsaicin, improve the glutathione peroxidase and catalase activity and glutathione content in the livers of the mice after gavage of capsaicin, reduce the malondialdehyde content, reduce the level of P substances and calcitonin gene-related peptides in serum of the mice after gavage of capsaicin, And tissue damages such as intestinal villus exfoliation and colitis cell infiltration caused by capsaicin are improved. The lactobacillus reuteri CCFM1175 disclosed by the invention is used for preparing pharmaceutical compositions and fermented foods for relieving pungency and protecting intestines and stomach, and has a very wide application prospect.

The invention discloses an application of Ferrostatin-1 and a derivative thereof in a medicine for treating or preventing ionizing radiation damage, and relates to the technical field of medicines. The invention specifically discloses the application of Ferrostatin-1 and the derivative thereof in preparation of the medicine for preventing / treating radioactive intestinal injury, radioactive bone marrow injury and body DNA injury caused by ionizing radiation. The Ferrostatin-1 and the derivative thereof provided by the invention have a protective effect on natural ionizing radiation and artificial radiation, and can be used as the medicine for preventing living body injury caused by radioactive substance radiation, nuclear substance leakage, space ionizing radiation and tumor radiotherapy. The invention provides a new idea for the application of Ferrostatin-1, and provides a new reference for medicines for treating or preventing ionizing radiation damage.

The invention relates to an in-vitro model construction method for rapidly screening macromolecular immunocompetent substances, which comprises the following steps: preparing a qualified conditioned culture medium, and stimulating intestinal epithelial cells by using the conditioned culture medium; then simultaneously adding a sample to be detected (upper chamber) and immune perception cells RAW264.7 (lower chamber) into Caco-2 intestinal epithelium monolayer cells for co-culture and incubation; after incubation, discarding the culture medium in the upper chamber, and adding a sample to be detected and a conditioned culture medium into the upper chamber; collecting the lower room culture medium, and detecting the level of inflammatory factors. According to the method, intestinal epithelial cells and immune cells are co-cultured to simulate the environment in the intestinal tract, and a sample to be screened and a conditioned culture medium jointly act on the epithelial cells on the upper layer to simulate the intestinal injury and organism immune regulation effect of food macromolecules in the intestinal tract.

The invention relates to the field of biomedicine, in particular to application of vitamin D in preparation of an anti-radiation drug. The application has the beneficial effects that the new anti-radiation application of the vitamin D is found and verified for the first time, and the vitamin D can significantly prolong the lifetime of a mouse after radiation and has outstanding effects of resisting intestinal tissue injury caused by radiation.

A method for preventing or reducing the risk of perinatal or neonatal morbidity and mortality caused by antenatal inflammation in humans is described. This method is based on the administration of a compound of formula I or a pharmaceutically acceptable salt thereof:to an expectant human mother suffering from antenatal fetal inflammation. The method prevents or reduces the risk of organ damages, including brain, lung and intestinal damages, and sequelae therefrom, in the neonates, as well as the risk of neonatal death.

The invention belongs to the technical field of medicine, and relates to application of indolepopionic acid to preparation of drugs for preventing and / or treating tumor radiotherapy side effect. Through research, the fact that indolepopionic acid can obviously improve intestinal injury of mice, caused by gama ray irradiation, is found, the improvement shows that the survival rate of laboratory mice is obviously improved, the weight is risen again, the intestinal inflammation level is obviously reduced, and paradoxical reaction of the laboratory mice does not occur, the fact that indolepopionicacid can be effectively applied to the prevention and / or treatment of tumor radiotherapy side effect is shown, and the indolepopionic acid is low in toxic and side effect, and effective before and after being taken, and has a wide application potential.

Disclosed are methods of assessing the ability of a candidate therapeutic agent to reverse, reduce or prevent intestinal injury by a potential toxic agent using a three-dimensional, engineered, bioprinted, biological intestinal tissue model. Also disclosed are methods of assessing the effect of an agent on intestinal function, the method comprising contacting the agent with a three-dimensional, engineered, bioprinted, biological intestinal tissue model.

The invention discloses a multifunctional rectal lifting device. The multifunctional rectal lifting device comprises a bent guide rod, a bent lifting rod and a movable handle; the lifting rod is rotationally connected with the guide rod; the guide rod is rotationally connected with the movable handle; the lifting rod is provided with a medicine injection hole and a first conveying mechanism for conveying medicines to the medicine injection hole; the multifunctional rectal lifting device further comprises a transmission mechanism for driving the lifting rod to swing relative to the guide rod; and the transmission mechanism is connected with the lifting rod and the movable handle, so that the movable handle can drive the lifting rod to swing. The bent lifting rod and guide rod meet a bent physiological status of the rectum, so that the position is effectively adjusted; the lifting rod can be adjusted by the transmission mechanism in operation to finish up-down and left-right actions on the rectum so as to reach the best operation position; gaps around the rectum are fully exposed, so that the effectiveness and reliability are enhanced, the surgical operation is facilitated, and the operation efficiency is improved; and further, the device has the function of injecting the medicines while adjusting the position of the rectum, so that the rectal injury can be reduced, the usabilityand convenience are enhanced, and the operation risk is lowered.

The invention relates to an establishment and use of a carbon tetrachloride induced freshwater fish acute liver and intestine combined injury model, and belongs to the technical field of aquaculture. The freshwater fish acute liver and intestine combined injury model is established and induced by adopting carbon tetrachloride. The model is applied to researches of liver and intestine injury mechanisms of fish and screening and development of drugs for liver and intestine protection of fish. The method for inducing the fish liver and intestine injury model in vivo by adopting the carbon tetrachloride solution has a minimum influence on non-specific immunity of fish, and is stable, reliable and good in reproducibility due to the fact that pathological expressions of liver and intestine inflammation are most suitable.

The invention relates to a traditional Chinese medicine composition for treating radioactive injury and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following 15 raw medicinal materials: radix rehmanniae recen, radix ophiopogonis, radix scrophulariae, codonopsis pilosula, scutellaria baicalensis, thunberg fritillary bulb, blackberry lily, pinellia ternate, white paeony root, salvia miltiorrhiza, moutan bark, mangnolia officinalis, poria cocos, mint and coptis chinensis. The composition in the invention is mild in medicine property, precise and appropriate in compatibility and synergistic in effect, has the effects of clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, nourishing lung and kidney and replenishing qi to invigorate the spleen, and is suitable for diseases such as mouth dryness, pharyngalgia, toothache, dry cough and chronic rhinitis caused by acute and chronic radioactive injuries and radiotherapy. Pharmacodynamic tests show that the composition disclosed by the invention has an exact treatment effect on radiation-induced lung injury and radiation-induced intestine injury.

The invention discloses a preparation for preventing and treating black body color missing symptoms of pelteobagrus fulvidracos due to poor feed freshness. The preparation is prepared from the following methods that the traditional Chinese medicine raw materials including herba ecliptae, ficus carica, cortex moutan, flastem milkvetch seeds and fructus cnidii are used; A, medicine juice decoction is performed: the herba ecliptae, the ficus carica, the cortex moutan, the flastem milkvetch seeds and the fructus cnidii are added into water for decoction; medicine juice is filtered and taken; then, chlorpheniramine maleate is added into the medicine juice; the medicine juice is concentrated to obtain a mixed solution A; B, coating is performed: the mixed solution A is coated to obtain the preparation for preventing and treating black body color missing symptoms of the pelteobagrus fulvidracos due to poor feed freshness. The preparation can be used for effectively preventing and treating the black body color missing occurring symptoms of the pelteobagrus fulvidracos due to fish meal staleness in feed, and also achieves a better repairing effect on stomach and intestine injury due to fish meal staleness. Therefore wide application prospects are realized.

The invention belongs to the field of biology, and relates to the application of serum ssc-miR-194b as a molecular marker for detecting intestinal stress damage in piglets. The nucleotide sequence of serum ssc-miR-194b is shown in SEQ ID NO.1, reversed The nucleotide sequence of the stem-loop primer required to record ssc-miR-194b is shown in SEQ ID NO.2, and the nucleotide sequence of the forward primer required to amplify ssc-miR-194b is shown in SEQ ID NO. As shown in 3, the above primers can be used to diagnose the intestinal stress injury of weaned piglets. The diagnostic method includes the extraction of serum total RNA, the isolation of small molecule RNA, cDNA synthesis, PCR amplification and identification. It provides guidance for the prevention, diagnosis and treatment of tract stress injury, and provides a new molecular marker for marker-assisted selection of pig stress-resistant strains.