314results about How to "Simple experiment" patented technology

The invention belongs to the field of molecular biology and relates to a DNA (deoxyribonucleic acid) library and a preparation method thereof, as well as a DNA sequencing method and device. The preparation method of the DNA library comprises the following steps of: (1) randomly disrupting genomic DNA of a sample into a 20-50kb DNA fragment; (2) performing the following step A or B: (A) filling two tail ends of the disrupted DNA fragment, adding capture marks and then separating the 20-50kb DNA fragment; (B) separating the disrupted 20-50kb DNA fragment, then filling the two tail ends of the DNA fragment and adding the capture marks; (3) performing cyclization on the separated DNA fragment to get annular DNA and removing the non-cyclized DNA fragment; (4) disrupting the annular DNA to a 100-2000bp DNA fragment; and (5) separating the DNA fragment with the capture marks from the DNA fragment obtained in the step (4) so as to get the a captured fragment. The preparation method of the DNA library, disclosed by the invention, has the advantages of being simple, fast and the like.

The invention discloses a method for evaluating the simulation imaging of a multi-mode SAR (Synthetic Aperture Radar), which mainly solves the problems of poor adaptability to different operating modes, complex experiment process and long experiment time in the prior art. The method comprises the following steps of: firstly, selecting an operating mode, inputting the parameters of a radar and the motion parameters of a carrier, loading a simulation scene and setting coordinates; secondly, setting a simulation radar echo signal according to a system environment; thirdly, imaging the radar echo signal to obtain a slant-range image; fourthly, geometrically correcting the slant-range image by using a three-dimensional geometric correction method to obtain a ground-range image; and finally, evaluating the quality of the ground-range image, and feeding back and correcting system parameters according the evaluation results so as to provide a feasible basis for a latter semi-physical simulation experiment. The method is applicable to three operating modes including a side looking mode, a slant looking mode and a scanning mode; only a plurality of parameters need to be input during operation, so that the experiment process can be simplified, the experiment time is saved, and the method can be used in a ground simulation experiment of SAR system design.

The invention relates to a synthesizing method of inorganic perovskite nanosheets. The method includes: adding the precursor solution of cesium into the precursor solution of lead halide, performing ultrasonic treatment for 10-30 minutes to obtain a mixed solution, transferring the mixed solution into a reaction kettle, performing reaction under 60-150 DEG C for 30-420 minutes, and naturally cooling to obtain reactants; performing centrifugal processing and washing to obtain the inorganic perovskite CsPbX3 nanosheets. The method has the advantages that the method is simple to operate and does not need harsh experiment conditions, and the prepared nanosheets are uniform in size, high in controllability and high in yield, and the quantum efficiency of the nanosheets reaches up to 48%.

The invention relates to atom laser cooling and atom laser capturing, in particular to a bi-color magneto-optical trap method and device for cooling and capturing atoms through lasers. According to the bi-color magneto-optical trap method and device, atom cooling and atom capturing are achieved through the bi-color lasers which work in a base-state, middle-excited-state and higher-excited-state cascading energy level, and atom pre-cooling is not needed. A step-type bi-color magneto-optical trap technology is adopted for the bi-color magneto-optical trap device, and the atoms can be directly cooled and captured from a vacuum air chamber. The step-type bi-color magneto-optical trap device can be used for cooling and capturing the alkali metal atoms, the alkaline earth metal atoms and even the rydberg atoms through the lasers; some quantum coherent effects can be directly researched in the cold atoms based on step-type bi-color dual-photon cooling, for example, the problem that correlation photon pairs are directly generated in bi-color magneto-optical traps is researched based on the rhombus-energy-level four-wave mixing effect; as for the experimental device, a bi-color magneto-optical trap experiment idea which is quite flexible and easy to achieve is initiated, and the experimental device can be conveniently applied and popularized.

The invention discloses a method for carrying out deceleration and monomolecular capture on a nucleic acid molecule based on a solid-state nano hole. The method comprises the following steps of: adding the nucleic acid molecule to be measured into a nano hole sequencing device filled with an electrolyte, wherein the nano hole sequencing device comprises an electrolytic bath provided with an anode and a cathode, and a solid-state nano hole thin film for separating the anode of the electrolytic bath from the cathode of the electrolytic bath; placing the nucleic acid molecule in a positive chamber of the electrolytic bath; during measurement, introducing a pressure external field into the positive chamber as a driving external field for the perforation of the nucleic acid molecule; applying a voltage between the anode and the cathode as an electric field external field reverse to the pressure external field; and in a measurement process, enabling the acting force of the pressure external field, from which the nucleic acid molecule is suffered in the nano hole to be more than or approximately equal to electric field force according to a deceleration purpose or a capture purpose to be realized by the method. By using the method, on the premise that the signal-to-noise ratio of a measurement signal is not decreased, the speed at which a DNA (deoxyribonucleic acid) molecule penetrates through a nano hole device can be effectively decreased, and a perforation speed can be slowed down for above one order of magnitude.

The invention discloses a preparation method of a two dimensional material transverse heterojunction. The preparation method of a two dimensional material transverse heterojunction includes the following steps: (a), taking material A powder as the original materials, performing vapour deposition on the surface of a substrate, and forming a nanometer sheet of the material A; and (b), heating the powder of the material B1 to the growth temperature under the reverse carrier gas flow, wherein the powder of the material B1 are horizontally growing along the rim of the nanometer sheet of the material A under the positive carrier gas flow, growing in the horizontal direction, and after completion of growing, the reverse carrying gas flow is imported again to prepare a two dimensional material transverse heterojunction A-B1. The invention also provides the two dimensional material transverse heterojunction prepared by the preparation method, and application of the preparation method in preparation of photoelectric devices. The related heterojunction prepared by the preparation method of a two dimensional material transverse heterojunction has the advantages of being smooth in the atomic layer, being steep and flat in the heterojunction boundary line, almost having no doping, and being excellent in optical and electrical properties of the material. The preparation method of a two dimensional material transverse heterojunction greatly simplifies the preparation method of the two dimensional material transverse heterojunction, and controllability of material preparation and the preparation efficiency are greatly improved.

The invention discloses a method for reducing perforation speed of nucleic acid molecules in a nanopore sequencing method. The method comprises the following steps of: adding the nucleic acid molecules to be measured into a nanopore sequencing device filled with electrolyte, wherein the nanopore sequencing device comprises an electrolytic cell provided with an anode and a cathode, and a solid nanopore film for separating the anode from the cathode of the electrolytic cell; placing the nucleic acid molecules to be measured in a cathode cavity of the electrolytic cell,; and when the nucleic acid molecules are measured, applying the voltage between the anode and the cathode, and introducing a reverse external field taking a pressure intensity external field as an electric field simultaneously. According to the method, the pressure intensity external field is reversely applied under the drive of an additional electric field, so that the speed of DNA (Deoxyribonucleic Acid) molecules is reduced when the DNA molecules pass through the solid nanopore, and the speed is reduced by about 50% to 80%, thus the time resolution of a DNA unimolecule detection technology is greatly improved. The method has very bright application prospect on a solid nanopore DNA molecule sequencing device.

The invention discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent comprises bacillus, an embedding carrier and a cross-linking agent, wherein bacillus is immobilized in the embedding carrier via the cross-linking agent. The preparation method comprises the following steps: inoculating bacillus into a culture medium containing phenanthrene to undergo aerobic culture, thus obtaining a bacterial liquid; concentrating the bacterial liquid, adding the concentrated bacterial liquid to the embedding carrier to obtain a mixed liquid containing the bacterial liquid, and then dropwise adding the mixed liquid containing the bacterial liquid in the cross-linking agent to undergo cross-linking hardening to form a bacillus gel pellet; then carrying out aerobic culture, thus obtaining the compound microbial agent. The compound microbial agent can quickly and efficiently degrade high-concentration phenanthrene in wastewater under the condition that the wastewater contains high-concentration Pb<2+>. The preparation method has the advantages of simple process, low production cost, and the like.

The invention discloses a CsPbX3@TiO2 nanomaterial as well as a preparation method and application thereof. The preparation method comprises the following steps: first, dissolving CsX and PbX2 in a surfactant and an N,N-dimethylformamide solution system, and heating to form a CsPbX3 precursor solution; then hydrolyzing and coating TiOx onto the surface of CsPbX3 in a titanate solution through a sol-gel method to obtain CsPbX3@TiOx; calcining under the protection of argon to obtain the CsPbX3@TiO2 nanomaterial. The CsPbX3@TiO2 nanometer material with an obvious structure and uniform coating isprepared with a 'one pot method', and has high fluorescence stability. The material powder can be placed stably at room temperature for two months or more, keeps ultrasonically stable in an aqueous solution for 60 minutes or more, and can be widely applied in various fields such as solar cells, photocatalysis, display devices, lasers, light detection devices and biological fluorescence labels.

The invention relates to a cetirizine molecularly imprinted polymer monolithic column and a preparation method thereof. The cetirizine molecularly imprinted polymer monolithic column is composed of raw materials of, by mass, 1-3% of cetirizine, 3-4% of 4-vinyl pyridine, 20-25% of ethylene gylcol dimethacrylate, 42-45% of mixed solution (eutectic solvent) of ethylene glycol and choline chloride, 4-5% of dimethylformamide, 13-22% of 1-butyl-3-methylimidazolium tetrafluoro borate and 0.5-1% of azobisisobutyronitrile. The method includes the steps of adding ionic liquid and the eutectic solvent of ethylene glycol and choline chloride to a polymerization system, using cobalt ions as a metal ion hub to enhance the effect of imprinting recognition, and preparing molecularly imprinted polymer (MIP) of a successive rod shape in a stainless steel column. The cetirizine molecularly imprinted polymer (MIP) monolithic column obtained through the preparation method has the advantages of good permeability and obvious imprinting effects and can have the model cetirizine imprinting factors up to 31.54, and meanwhile, the method is simple in preparation process and avoids the use of volatile liquid, thereby reducing harmful gas emission to the environment.

The invention discloses a preparation method for a NiCoP nano material. The preparation method comprises the following steps: dissolving a nickel salt and a cobalt salt into a beaker with deionized water in proportion, and stirring until the nickel salt and the cobalt salt are completely dissolved; then, heating and evaporating on the stirrer until the nickel salt and the cobalt salt are completely dried to obtain a product; putting the product into a muffle furnace, and roasting to obtain black powder; sufficiently mixing the black powder with a certain amount of phosphor salt, and heating in the presence of N2 in a high-temperature tubular furnace to react, thereby obtaining a NiCoP nano material crude product; respectively washing the NiCoP nano material crude product with deionized water and absolute ethyl alcohol in sequence, and putting the washed product into a vacuum drying oven, and drying, thereby obtaining the NiCoP nano material. The preparation method has the advantages of being low in energy consumption, low in cost, simple and convenient in reaction condition, high in safety coefficient, and the like.

The invention relates to a method for manufacturing a nitrogen-doped multi-wall carbon nano tube. The method comprises the following steps: uniformly mixing a mesoporous silica mold plate and a nitrogenous macrocyclic iron complex in a physical mechanical mode so as to obtain a mixture, wherein the mesoporous silica mold plate is of a three-dimensional cubic open-framework structure and a surfactant is not removed, and the nitrogenous macrocyclic iron complex can be simultaneously used as a carbon source, a nitrogen source and an iron source; carrying out heat treatment on the mixture so as to prepare an iron oxide / N / carbon nano tube / silicon dioxide mold plate composite material; removing the silicon dioxide mold plate of the composite material through strong alkali; removing the iron oxide of the composite material through strong acid so as to obtain the nitrogen-doped multi-wall carbon nano tube. By utilizing the method, complex steps the conventional manufacturing process of the nitrogen-doped multi-wall carbon nano tube are effectively avoided, the method is simple, convenient and rapid, and time and labor are saved.

The invention discloses a polycamacrylacid ester resin making method of modified aminosilane, which comprises the following steps: dripping epoxy radical silane in the primary amino radical silane at 40-100 deg.c; finishing dripping within 15-60 min; reacting for 1-3 h at 40-100 deg.c to produce modified silane coupling agent; blending polyether polyol and hydroxyl acroleic acid resin; extracting into vacuum; adding isocyanate in the mixture at 20-50 deg.c; heating to 60-100 deg.c for 1-3h after radiating; producing endisocyanates prepolymer; dripping the modified silane coupling agent in the prepolymer for 1-3 h at 60-100 deg.c to produce the product.

The invention belongs to the electrochromic thin film preparation technologies, and relates to an electrochemical doping method for an electrochromic thin film. The steps for electrochemical doping comprise preparation of the electrochromic thin film, preparation of doped liquid, preparation before doping and process of doping. According to the electrochemical doping method for the electrochromic thin film, high-proportion doping is achieved; deposit cannot be generated in the doping process so that the uniformity of the doped thin film can be ensured; and when multi-component doping is conducted, the experimental process is simplified, expensive experimental equipment is omitted, and therefore the doping cost is reduced.

The invention relates to a super-capacitor fractional order model parameter identification method. The method comprises the steps of 1, experimentally collecting resonant frequency response data of a series resonant circuit containing a super-capacitor; 2, analyzing and calculating the resonant frequency response data of the series resonant circuit containing the super-capacitor; and 3, according to a fractional order calculus theoretical derivation formula, performing identification to obtain the capacity and a fractional order of the super-capacitor. According to the method, based on the scientific fact that model parameters of actual capacitance and inductance are all fractional order parameters, by adopting an experimental method for measuring fractional order series resonance frequency response of the super-capacitor, and through building a super-capacitor shunt Ro C alpha fractional order impedance model, resonant frequency response experiment and numerical calculation are carried out, a super-capacitor fractional order model parameter identifier is designed and manufactured, and accurate parameters of a super-capacitor fractional order model are obtained; and the experiment is simple and easy, the steps are concise, a calculation result is accurate, and the applicability is wide.

The invention provides a preparation method of high purity trifluridine. The method is as below: reacting a raw material of a compound 2 with HMDS under the action of trimethylchlorosilane to obtain a compound 3; subjecting the compound 3 and a compound 4 to condensation in the presence of a catalyst copper difluoride; conducting ethanol recrystallization to obtain a compound 5; and finally conducting deprotection on the compound 5 under the effect of sodium methylate; and recrystallizing by a mixed solvent of ethanol and acetone (1:1) to obtain the high purity object compound 1. The method has the advantages of high purity of the product, simpleness, easy purification and less industrial pollution.

The invention provides a lithium-rich manganese-based hollow nanosphere cathode material formed by winding slices and a preparation method thereof. A molecular formula of the cathode material is xLi<2>MnO<3>.(1-x)LiNi<1 / 3>Co<1 / 3>Mn<1 / 3>O<2>, wherein the x is greater than 0 and is smaller than 1. The preparation method comprises the steps of preparing polystyrene spheres a; sulfonating the polystyrene spheres a, thereby obtaining sulfonated polystyrene spheres a; dissolving nickel salt, cobalt salt, manganese salt and hexamethylenetetraminein into sodium citrate solution, thereby obtaining solution c; dispersing the sulfonated polystyrene spheres a in the solution c, and carrying out absorption, centrifugation, washing and drying to obtain powder d; grinding and mixing the powder d and lithium salt to obtain powder e; and carrying out high temperature calcination on the powder e, thereby obtaining the cathode material. According to the cathode material prepared by the invention, the first-time discharge capacity under 0.1C reaches 281.7mAh g<-1>, the first-time discharge capacity under 10C reaches136.6mAh g<-1>, and after circulation of 200 circles, a capacity retention ratio under 10C is 70%.

The present invention discloses preparation process of acrylate modified gamma-aminopropyl triethoxysilane. The preparation process includes: dropping acrylate monomer into primary amino gamma-aminopropyl triethoxysilane at 40-100 deg.c within 15-60 min, maintaining the temperature to react for 1-3 hr to obtain modified silane coupler, mixing polyether polyol and hydroxyl acrylic acid and vacuumizing to obtain mixture, adding diisocyanate into the mixture to produce exothermic reaction, heating to 60-100 deg.c after exothermic reaction to react for further 1-3 hr to obtain the pre-polymer of terminal isocyanate, dropping the modified silane coupler into the pre-polymer to react at 60-100 deg.c for 1-3 hr to obtain the acrylate modified gamma-aminopropyl triethoxysilane. The present invention has smooth modification process, simple operation and low cost, and the obtained modified silane coupler has low viscosity and high stability.

The invention discloses a multi-spindle machine tool geometric error identification method based on ball bar measurement. The method comprises the following steps of determining the mounting positionof a ball bar according to the structure of a machine tool and the position of a rotation shaft, and then independently detecting position-independent geometric errors of the rotation shaft; identifying position-independent geometric errors of a linear shaft in a mode of combination of the linear shaft and the rotation shaft; and obtaining the relation between the coordinates of the linear shaft and the angle position of the rotation shaft in combination of a specific detection track, establishing a position-independent geometric error identifying matrix of the rotation shaft and the linear shaft through a homogeneous change matrix, and then decoupling each position-independent geometric error. The position-independent geometric errors of the five-spindle machine tool are identified through comparison of experiment results and a simulation result. Detection equipment only needs to be installed once during the detection steps, measurement is convenient, and the identifying precision ishigh.

The invention depicts a portable gene detecting device integrating nucleic acid extraction, specific target nucleic acid amplification and detection. The integral device only needs to be uncovered once in the whole process during sampling adding and is then sealed so that target nucleic acid amplification products can be prevented from leaking out and causing contamination. The device conducts detection and result judgment and read in the visible form of naked eyes, is easy to operate, wide in sample application range and convenient to carry, can be used for detection on the first site of epidemic situation, is suitable for early diagnosis of various pathogens and respiratory infectious disease, and is used for screening out possible nucleotide sequences of genetic disease or infectious disease and monitoring infectious disease treatment efficiency.

The invention discloses a nucleic acid double-chain cyclization method, a methylation sequencing library construction method and a kit. The nucleic acid double-strand cyclization method comprises the following steps: providing a forward primer and a reverse primer, wherein each of the forward primer and the reverse primer comprises a U basic group, the nucleotide number difference of the upstream sequences of the 5'ends of the U basic groups of the forward primer and the reverse primer is N, and the upstream sequences of the 5 'ends of the U basic groups of the forward primer and the reverse primer are at least partially and reversely complementary; amplifying the nucleic acid fragment by using the primer pair to obtain an amplification product; a USER enzyme is used for cutting off U basic groups in the amplification product, then a mononucleotide gap is formed, chain dissociation occurs to the residual nucleotide sequence from the gap to the 5'end due to the unstable structure, and then partially complementary cohesive terminal structures are formed at the two ends of the amplification product; the sticky tail end structures at the two ends are complementarily connected to form a double-chain structure, one chain of the double-chain structure is closed, and the other chain of the double-chain structure has N deleted basic groups. The method is used for constructing the WGBS library, and the phenomenon that the methylation rate of the WGBS library is high due to single-chain cyclization deviation can be reduced.

The invention discloses a rapid analysis device and method for ambient air volatile organic compounds. The rapid analysis device comprises an ambient air sampling bottle, a sample leading-in mechanism and a reformed atmosphere pressure chemical ionization tandem mass spectrometer. The rapid analysis method includes: using the ambient air sampling bottle to directly collect the ambient air, transferring the sampling bottle to a laboratory, directly leading the ambient air without concentration treatment into an ion source through an air manual switching four-way valve to be performed with rapid analysis through a reformed atmosphere pressure chemical ionization mass spectrometry / mass spectrometry (APCI-MS / MS). Compared with a traditional method for analyzing the volatile organic compounds in the ambient air, the ambient air sampling bottle and the sample leading-in mechanism in the method are simple in structure and convenient to operate, the iron source can achieve direct leading in of the air samples through the reformed APCI-MS / MS, the process from sample induction to data collection only needs several seconds, the sensitivity is high, the within-day precision and day repeatability are good, and the rapid analysis device and method are suitable for rapid analysis of the trace volatile organic compounds in the ambient air.

The invention discloses a DNA nanostructure, an electrochemical aptamer biosensor system as well as a preparation method and application of the electrochemical aptamer biosensor system, and relates tothe field of food-borne pathogenic bacteria detection. A stable DNA nanostructure is synthesized through a rolling circle amplification reaction, and heme is loaded to serve as an electrochemical signal label to amplify a signal; the single-stranded DNA capture probe is modified on the surface of the Au electrode through an Au-S bond; the food-borne pathogenic bacteria specific aptamer and the capture probe form a double-stranded DNA structure on the Au electrode; the aptamer is preferentially combined with the food-borne pathogenic bacteria, so that the aptamer-capture probe double-chain body is dissociated, and the capture probe is released; the free capture probe is hybridized with the DNA nanostructure through a complementary DNA sequence; and the concentration of the food-borne pathogenic bacteria is quantitatively determined through an electrochemical signal of a DPV method. The method is simple, rapid, easy to operate, low in experiment cost, sensitive, high in specificity andgood in application prospect.

The invention belongs to the field of a material coating, and particularly provides a new method for preparing a high-quality diamond coated cutting tool by multilayered step-by-step deposition. The new method concretely comprises the following steps: firstly, plating a molybdenum layer on the surface of a hard alloy cutting tool by adopting an electroplating method or a physical vapor deposition method, wherein molybdenum is bound together with cobalt on the surface of a matrix to form a stable compound so as to form a transition layer on the surface of the matrix, so that not only can the influence of cobalt on the surface of the cutting tool to the diamond coating be reduced, but also the dispersion of cobalt towards the surface of the cutting tool from the depth position of the matrix is prevented; and then depositing a diamond coating by two steps through utilizing microwave plasma CVD (MPCVD) method, namely controlling corresponding parameters in diamond nucleation and growth stages to form a nanodiamond thin film with high quality and high stability.

The invention discloses an apparatus for environment corrosion cracking test in a wet hydrogen sulfide environment. The apparatus can realize environment corrosion cracking test at a temperature lower than 75 DEG C in an enclosed circulating solution environment filled with hydrogen sulfide with different concentrations and gas mixtures thereof. The apparatus comprises a corrosion solution storing and circulating container, a solution circulating system, an environment corrosion cracking test sample room and an electrochemical auxiliary device, wherein the corrosion solution storing and circulating container mainly stores a great amount of a solution, the front end of the corrosion solution storing and circulating container can be additionally provided with a gas mixture interface or a gas mixture flowmeter to allow the solution to have different concentrations of hydrogen sulfide, carbon dioxide or the like, the solution circulating system comprises a magnetic drive pump, a rubber ball and a ball valve to realize siphonage at the front end and cut-off of communication with the environment corrosion cracking test sample room, and the environment corrosion cracking test sample room mainly comprises an electrochemical test interface and is used for sealing of a sample and circulation of the solution. The apparatus provided by the invention can substantially reduce leakage of hydrogen sulfide and the solution thereof, improves security of test personnel and environment and is convenient to use and applicable under a wide range of test conditions.

The invention provides a preparation method for a Li<2>Fe<x>Mn<1-x>SiO<4>@graphene hollow nanosphere cathode material. The method comprises the specific steps of taking polystyrene spheres (PS) as templates; coating a layer of uniform mesoporous silica on the surfaces of the templates; ultrasonically dispersing the templates into a certain molar ratio of alcoholic solution of lithium salt, ferric salt and manganese salt; carrying out oil bathing, stirring, absorption, centrifugation, washing and drying to obtain Li<2>FeSiO<4> precursors formed on the surface of the polystyrene spheres, slowing adding the Li<2>FeSiO<4> precursors into graphene water solution, carrying out stirring and freeze drying, and carrying out high-temperature calcination in inert gas, thereby obtaining grey powder and obtaining the prepared Li<2>Fe<x>Mn<1-x>SiO<4>@graphene hollow nanosphere cathode material. According to the method, the raw material content is abundant, the cost is low, the prepared Li<2>Fe<x>Mn<1-x>SiO<4> cathode material hollow nanospheres are distributed uniformly, an excellent microstructure is achieved, and the relatively good electrochemical performance is achieved.

The invention discloses a method for preparing a carbon coated nano flaky tin oxide material. A carbon coated nano flaky tin oxide (SnO2 / C) cathode material can be prepared by using an improved one-step hydrothermal method, that is, a complexing assisting-pre-precipitation-hydrothermal method. The method is simple and convenient, preparation steps of a conventional two-step hydrothermal method coated material are simplified, the carbon coated nano flaky tin oxide (SnO2 / C) composite lithium ion electrode cathode material prepared by using the method is beneficial to relieving the problem that the volume of a single tin oxide nano electrode is expanded in the charge / discharge process, the circulation property of the battery is improved, the specific capacity of the battery is increased, and the service life of the battery is prolonged. At the room temperature, the first discharge capacity of the battery at the 0.5C multiplying power is up to 757mAh / g. The method and the structure have important significance in application of lithium ion battery electrode materials.

The invention discloses an attapulgite-contaiing composite nanofiltration membrane and a making method thereof. The nanofiltration membrane contains attapulgite with a length / diameter ratio of 20 or less, the attapulgite has a rod-shaped or fibrous structure, and has hollow nanometer tunnels, and the diameter of the attapulgite is 10-20nm. The method comprises the following steps: 1, a porous support membrane contacts with a water phase solution containing a reaction monomer and the attapulgite; 2, the porous support membrane infiltrated in the water phase solution is rolled to remove the superfluous water phase solution; 3, the porous support membrane obtained after the processing in step 2 contacts with an organic phase solution containing another reaction monomers to obtain a composite membrane; and 4, the composite membrane obtained in step 3 is dried, the dried composite membrane undergoes heat treatment, and the obtained composite membrane is washed by water to obtain the attapulgite-contaiing composite nanofiltration membrane. The making method greatly improves the nanofiltration flux of the composite nanofiltration membrane, maintains the high retention rate of the composite nanofiltration membrane, and substantially reduces the application running cost of the composite nanofiltration membrane.

The invention discloses a high-flux visible chip detecting method of protein-protein interaction and a detecting kit for protein-protein interaction. The method comprises the steps as follows: a Flag antibody chip is prepared; cell lysis solution with Flag-bait fused protein and Myc-quarry fused protein is added onto the surface of the chip for co-immunoprecipitation; and existence of interaction between the bait protein and the quarry protein is detected by the combination of colloidal gold and the silver enhancement colour technology. The kit comprises an aldehyde galssslide with a plurality of sub-areas, a FLAG, a c-Myc label carrier, a monoclonal antibody corresponding to the c-Myc label vector, chain-avidin colloidal gold, silver enhancement solution A and B, and a specification based on the method. The high-flux visible chip detecting technology of protein-protein interaction established in the invention is an efficient and effective tool for proteomics research.

The invention provides a method for simulating the output data of an energy spectrum CT system. The method comprises the steps of establishing a detector array on a simulation platform; setting an X-ray light source and a body model; setting a physical module, and obtaining the total number of electron-hole pairs after collision according to the physical module; and verifying the total number of electron-hole pairs after collision via body model imaging. By adopting the method provided by the invention, the simulation speed and the simulation accuracy of particle detection can not only be improved, the accuracy and the speed of generating medical data sets can also be improved to promote the development of the medical image processing field. By adopting the method provided by the invention, related research methods are enriched, the experimental process is further simplified, and the costs of human and material resources required for the experiment are reduced.