Gene detecting device integrating extraction, amplification and detection

A gene detection and cylinder technology, which is applied in the general fields of molecular biology and medicine, can solve the problems of inability to directly observe the detection results, inconvenient to carry instruments, cumbersome steps, etc., so as to achieve no need for special equipment, and the operation is simple and easy to carry out. , the effect of simple operation

Active Publication Date: 2015-12-16
GUANGZHOU HEAS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] The purpose of the present invention is to overcome the disadvantages of the prior art that it is inconvenient to carry instruments, and the samples need to be extracted, amplified, and detected step by step in sequence, the steps are cumbersome and the detection results cannot be directly observed, and a method for quickly extracting target nucleic acids, Portable device with integrated amplification and detection
This device can be used for all nucleic acids and their derivatives

Method used

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  • Gene detecting device integrating extraction, amplification and detection
  • Gene detecting device integrating extraction, amplification and detection
  • Gene detecting device integrating extraction, amplification and detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1: Sample flow through a preferred portable device

[0084] The portable device has two medium and long cylinders, the first hollow long cylinder (1) is used for extracting and amplifying the target nucleic acid sequence, and the second hollow long cylinder (25) is used for detecting the nucleic acid sequence. The sample is added to the first compartment (12) located in the cylinder (1), the compartment (12) contains a dry lysis reagent for nucleic acid extraction, and the sample provides a liquid for resuspending the lysis reagent. Tighten the screw cap (2), after incubating for a period of time, remove the fixing ring (10) on the upper end of the screw cap (2), and slowly push the push rod (6) to the scale A, that is, there is a second fixing ring (11). ) position, at this time, the liquid flows into the filter system (14), after two minutes, remove the fixed ring (11), and push the push rod (6) to the bottom, at this time, the filtered nucleic acid flows into...

Embodiment 2

[0085] Example 2: Sample Extraction System

[0086] A non-limiting example of an extraction system can include:

[0087] Lysates used to concentrate buffer components of DNA or RNA samples, and may include specially treated multi-layer absorbents, as well as extraction solutions including but not limited to detergents and alkaline buffers.

[0088]This lysate disrupts cell walls or membranes and exposes genetic samples such as DNA or RNA to the solution. It is envisaged that proteases may be added to remove proteins and cell walls. Alternatively, separate solution-type coatings or multilayer films may be used.

[0089] In addition, various reservoirs, activated carbon filters, and ion exchange filters can be used. In addition, the filter can be a semipermeable membrane with a molecular cut-off ranging from 60,000 to 300,000 D or higher and can be non-stick coated.

[0090] The filtration system removes all potential inhibitors, small molecules, ions, and proteins.

[0091...

Embodiment 3

[0092] Example 3: Nucleic Acid Amplification

[0093] Nucleic acid isothermal amplification technology is characterized in that the whole process of the amplification reaction (except the initial hybridization step) is carried out at a single temperature, and does not need to be carried out under a special amplification instrument, unlike the PCR reaction, which needs to undergo dozens of temperature changes cycle process. This feature of the isothermal amplification technology enables it to support the isothermal amplification platform used in the portable device of the present invention, and the temperature required for the amplification is achieved by a heating loop.

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Abstract

The invention depicts a portable gene detecting device integrating nucleic acid extraction, specific target nucleic acid amplification and detection. The integral device only needs to be uncovered once in the whole process during sampling adding and is then sealed so that target nucleic acid amplification products can be prevented from leaking out and causing contamination. The device conducts detection and result judgment and read in the visible form of naked eyes, is easy to operate, wide in sample application range and convenient to carry, can be used for detection on the first site of epidemic situation, is suitable for early diagnosis of various pathogens and respiratory infectious disease, and is used for screening out possible nucleotide sequences of genetic disease or infectious disease and monitoring infectious disease treatment efficiency.

Description

technical field [0001] The present invention relates to the general fields of molecular biology and medicine, and in particular to the field of methods for extracting nucleic acids, amplifying specific target nucleic acids and detecting amplified nucleic acid sequences in a portable device. Background technique [0002] As public health impacts and awareness of the environmental reservoir of infectious and emerging diseases, biothreat agents, genetic diseases and causative agents have increased, there is a need for more informative, sensitive and specific use of rapid assays The demand for tools to detect samples has increased. At present, the nucleic acid is extracted by the traditional nucleic acid extraction method, and the molecular detection by the PCR amplification method is very sensitive, specific and informative. Unfortunately, currently available nucleic acid extraction and nucleic acid detection methods are not suitable or have limited utility for use at the samp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34
CPCC12Q1/6804C12Q2563/131C12Q2563/137C12Q2565/625
Inventor 陈华云刘淑园陈嘉昌肖湘文丁渭曾烨叶映玲黄爽彭俊然刘孝礼
Owner GUANGZHOU HEAS BIOTECH CO LTD
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