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Compound microbial agent as well as preparation method and application thereof

A technology of compound microbial inoculants and bacterial liquids, applied in the directions of microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc., can solve the problems of insoluble in water, limited application, prolonged degradation time, etc. Efficient degradation, strong treatment effect, good mass transfer performance

Inactive Publication Date: 2015-11-04
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But phenanthrene is insoluble in water, and it is not easy to be attached by bacillus in water, which prolongs the degradation time
At the same time, phenanthrene is also toxic to bacillus. Long-term large-scale exposure will reduce the activity of bacillus and weaken its degradation ability, which limits its application in practical engineering.

Method used

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  • Compound microbial agent as well as preparation method and application thereof
  • Compound microbial agent as well as preparation method and application thereof
  • Compound microbial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The Bacillus sp.P1 of the present embodiment is screened by the following method:

[0047] (1) Insert the bacillus (bacillus is a conventional bacillus) into the inorganic salt medium containing phenanthrene (the composition of the inorganic salt medium is: MnSO 4 0.0447mg / L, ZnSO 4 0.0686mg / L, (NH 4 ) 6 MO 7 o 24 4H 2 O0.0347mg / L, K 2 HPO 4 129.15mg / L, Na 2 HPO 4 167mg / L, KH 2 PO 4 43.5mg / L, NH 4 Cl 25mg / L, MgSO 4 13.8mg / L, CaCl 2 36.4mg / L, FeCl 3 0.42mg / L) in aerobic culture for 7d (150rpm, 30°C) to obtain the bacterial liquid.

[0048] (2) Take the bacterial solution in step (1) and drop it into the plate medium (the plate medium is beef extract peptone medium) for purification for 3 days.

[0049] (3) Taking steps (1)-(2) as a domestication cycle, a total of 6 cycles of domestication, the concentration of phenanthrene in the inorganic salt medium in each cycle is shown in Table 1, and other culture conditions are the same as those of the firs...

Embodiment 2

[0055] A composite microbial agent, the composite microbial agent includes Bacillus sp.P1 (KP888558), an embedding carrier and a cross-linking agent, and the Bacillus sp.P1 is fixed in the embedding carrier through a cross-linking agent to form a gel Bacterium balls (i.e. composite microbial agents), the embedding carrier is a mixed solution of polyvinyl alcohol and sodium alginate, and the cross-linking agent is boric acid and CaCl 2 mixed solution. Wherein, the Bacillus sp.P1 (KP888558) adopts the Bacillus sp.P1 of Example 1.

[0056] A kind of preparation method of the composite microbial bacterial agent of above-mentioned present embodiment, as figure 1 shown, including the following steps:

[0057] S1. Inoculate 25g of Bacillus sp.P1 (KP888558) into 500mL conical flask containing sterilized beef extract peptone medium containing phenanthrene concentration of 100mg / L by concentration of 5w / v%. The temperature is 37° C., and the rotation speed is 200 rpm in a constant te...

Embodiment 3

[0061] A kind of application of the compound microbial bacterial agent of embodiment 2 in degrading the high concentration phenanthrene in waste water, concrete application method comprises the following steps:

[0062] Add 5g / L phenanthrene acetone 1mL respectively in three groups (group 1, group 2 and group 3), add 50mL inorganic salt medium after the acetone evaporates to dryness (the composition of inorganic salt medium is: MnSO 4 0.0447mg / L, ZnSO 4 0.0686mg / L, (NH 4 ) 6 MO 7 o 24 4H 2 O 0.0347mg / L, K 2 HPO 4 129.15mg / L, Na 2 HPO 4 167mg / L, KH 2 PO 4 43.5mg / L, NH 4 Cl 25mg / L, MgSO 4 13.8mg / L, CaCl 2 36.4mg / L, FeCl 3 0.42mg / L).

[0063] After sterilization, add 15 mL of the bacterial solution BSP prepared in Step 1 of Example 2 to Group 1; add the composite microbial agent of Example 1 to Group 2, 5 g by wet weight; Group 3 is a blank without bacteria control group. Place the three groups of Erlenmeyer flasks in a constant temperature shaking box wi...

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Abstract

The invention discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent comprises bacillus, an embedding carrier and a cross-linking agent, wherein bacillus is immobilized in the embedding carrier via the cross-linking agent. The preparation method comprises the following steps: inoculating bacillus into a culture medium containing phenanthrene to undergo aerobic culture, thus obtaining a bacterial liquid; concentrating the bacterial liquid, adding the concentrated bacterial liquid to the embedding carrier to obtain a mixed liquid containing the bacterial liquid, and then dropwise adding the mixed liquid containing the bacterial liquid in the cross-linking agent to undergo cross-linking hardening to form a bacillus gel pellet; then carrying out aerobic culture, thus obtaining the compound microbial agent. The compound microbial agent can quickly and efficiently degrade high-concentration phenanthrene in wastewater under the condition that the wastewater contains high-concentration Pb<2+>. The preparation method has the advantages of simple process, low production cost, and the like.

Description

technical field [0001] The invention relates to the field of microbial preparations and wastewater treatment, in particular to a composite microbial bacterial preparation, a preparation method and its application in removing high-concentration phenanthrene in wastewater. Background technique [0002] With the rapid development of urbanization and industrial and agricultural production, the pollution of polycyclic aromatic hydrocarbons has become a serious environmental pollution problem, which has aroused the common attention of all countries in the world. Many industrial production activities such as coking, oil refining, metallurgy, and food processing can cause incomplete combustion of hydrocarbons and produce polycyclic aromatic hydrocarbons, which are widely present in the atmosphere, water, and soil, causing environmental pollution. PAHs have good stability, are difficult to be bioutilized, and can exist in the environment for a long time. The hydrophobicity and fat-s...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C12N11/04C12N1/36C02F3/34C12R1/07C02F101/32
Inventor 刘少恒曾光明牛秋雅王慧
Owner HUNAN UNIV
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