241 results about "Degradative enzyme" patented technology

A method of degrading a plastic in the presence of a biosurfactant; a method of degrading a plastic by contacting the plastic with a microorganism; a process for producing a useful substance from a plastic which comprises degrading the plastic by contacting the plastic with a microorganism and further converting the components of the thus degraded plastic with the use of a microorganism; a method of degrading a plastic by contacting the plastic with a microorganism in the coexistence of a biosurfactant and/or a plastic-degrading enzyme and thus degrading the plastic under the action of the microorganism; a transformant microorganism having been recombined with at least one DNA selected from among a DNA containing a gene encoding a surface active substance, a DNA containing a gene encoding a plastic-degrading enzyme and a DNA containing a gene encoding a useful substance; novel genes as described above; and proteins encoded thereby.

A system for performing arthroscopic lavage, directed tissue drying, and the accurate placement of a biocompatible tissue scaffold for the adherence of autologous regenerated cells through a small single port of entry into a joint compartment. The system is comprised of a handpiece having valves for irrigation and suctioning and a dual valve swivel cannula attached to the handpiece. The system includes a mobile cart, high resolution camera, light source, optical coupler, high-resolution monitor, an air compressor to power individually controlled irrigation pumps to deliver irrigation fluid to a handpiece and a vacuum suction console to collect fluid. The system also includes an insufflator to maintain distension immediately following the lavage and to dry tissue in preparation for directed tissue scaffold and regenerative cell placement. The delivery system achieves accurate biocompatible tissue scaffold placement to a specific tissue site or sites within the joint utilizing a small diameter arthroscope for direct visualization while inserting and advancing a grasping instrument or device through one of two valves located on the cannula. While holding the tissue scaffold in the jaws of the grasping device, it is advanced through the cannula lumen and extended beyond the distal tip and placed on the dried tissue site. Removing the grasping device, a catheter is then inserted and advanced through a cannula valve into the lumen and extended beyond the distal tip to the scaffold placed and prepared tissue site. A means of applying torque to the catheter tip further enhances the ability for accurate, exact placement of cells to a specific scaffold receptive tissue site. The cells are then injected into and through the catheter and applied under direct visualization to the scaffold. As comprised, the small single-port system allows a physician to perform the diagnosis, clean the joint space of debris and degradative enzymes using pressurized irrigation and suction, followed by a rapid conversion from a sterile saline fluid distension media to a dry gas CO2 distension media and directed tissue drying, and the accurate placement of a biocompatible tissue scaffold for the adherence and accurate placement of regenerated cells through a catheter to a specific tissue site within a joint.

The invention discloses a method for preparing organic seaweed liquid fertilizer by fermentation and enzymolysis of microorganisms and belongs to the field of fertilizer production. Seaweeds are degraded through a compound enzymolysis technology and a microorganism fermentation technology; a complicated enzyme system produced through microorganism fermentation and a compound enzyme including proteinase, cellulase, pectinase, polysaccharide degradation enzyme and the like are used for degrading the seaweeds step by step; seaweed cell walls are removed so that contents including alginic acid, polysaccharide, plant hormones and the like are sufficiently released; the decomposition of active substances including the alginic acid, plant growth promotion factors and the like is avoided under moderate technological condition; effective active components in the seaweeds are kept to the greatest extent. According to the method disclosed by the invention, energy saving, water saving, low carbonand environment protection are realized; the seaweed biological organic liquid fertilizer contains rich mineral substances and effective components including macro-elements, microelements and the likeneeded by the growth of plants; a soil structure can be improved to keep moisture and soil and the growth of root microorganisms of the plants is promoted; the yield of applied crops is remarkably improved and the fertilizer has a great market potential.

The invention discloses a method and usage of preparing natural absorbent with enzymatic hydrolysis. Adding abandoned biomass and degrading enzyme solution into a fermentation reactor, and the pH value of the enzymolysis buffer solution is 3 to 6 under a enzymolysis temperature of 30-50 DEG C, and the natural absorbent is prepared through washing, filtering, drying and grinding after 1 to 10 days of stirring enzymolysis in 20 to 200r/min; the degradation enzyme is cellulose of 0.5 to 8mg/L, pectinase of 0.5 to 8mg/L or the mixing solution of the cellulose and the pectinase with a proportion of 1 to 1; 10 to 100mL degradation enzymes are added into every gram of abandoned biomass raw materials, which enables 2 to 20IU of active enzymes to be added into every gram of biomass raw material. The prepared natural absorbent are used for removing a micro amount of persistent organic pollutants in wastewater or drinking raw water and absorbing liquid organic pollutants leaked in accidents. The method and usage of preparing natural absorbent with enzymatic hydrolysis has the advantages of simple preparation method, low cost and environmental protection; the absorbent has strong absorption power, large capacity without toxicity or harm.

The invention relates to a novel beta glucosidase/xylosidase difunctional cellulose degradation enzyme RuGBGX2 as well as a coding gene and application thereof. The coding sequence of amino acid of the RuGBGX2 contains 18-755th sites of an SEQ ID NO 2 sequence. The RuGBGX2 is sourced from the rumen microorganism of yak from China, a novel coding gene of the beta glucosidase/xylosidase difunctional cellulose degradation enzyme RuGBGX2 is obtained by function screening and sequencing analysis on a rumen metagenome cosmid library and a subclone library. The beta glucosidase/xylosidase difunctional cellulose degradation enzyme provided by the invention can be widely applied to the degradation of cellulose and the fields such as cellulose biotransformation, chemical industry, spinning, foods, bioenergy, feed additives, medical industry and the like. By utilizing the difunctional enzyme RuGBGX2 to degrade wood fiber, the varieties of added enzymes can be reduced, and an enzymolysis process can be simplified.

The disclosure provides, e.g., enucleated erythroid cells comprising an amino acid degradative enzyme such as asparaginase and a targeting moiety such as an anti-CD33 antibody molecule. The cells may be used, e.g., to treat cancers such as AML.

The invention relates to a preparation method and an application of a petroleum degrading enzyme preparation. The preparation method comprises the following preparation steps: performing cell breakage on microorganisms for degrading petroleum to prepare crude enzyme liquid; performing mixed adsorption on the crude enzyme liquid and a carrier; and separating and drying to prepare the petroleum degrading enzyme preparation, wherein the microorganisms for degrading the petroleum are acinetobacter calcoaceticus; the collection number of the strains is CGMCC No.3915; and the collection mechanism is Common Microorganism Center of China General Microbiological Culture Collection Center. The invention also comprises the application of the prepared petroleum degrading enzyme preparation to petroleum degradation. An enzyme system of the microorganisms with a petroleum degrading function is fixed by an adsorbent and then is used for degrading soil polluted by the petroleum, so that the degradation efficiency is obviously improved and is increased by 30 to 50 times compared with the microbial degradation speed, and the stability is improved by 15 to 20 times compared with that of the crude enzyme liquid.

The present invention relates to a novel tobacco leaf fermenting enzyme preparation production method which aims to solve the technical problems that how the quality of the tobacco leaf fermenting product is improved and how the reactivity protection of the tobacco leaf fermenting enzyme preparation is realized in the natural fermenting field of the tobacco leaf. The enzyme preparation consists of glucoseoxidase, chlorophyl oxidase, carotenoid oxidase, protease, and nicotine-degradation enzyme. Through the cell disruption of fresh leaves, (NH ₄) ₂ SO ₄ is utilized to operate the second fractional precipitation to obtain crude enzyme fluid, an enzyme molecule adopts Ca ^{2 +} and Mg ^{2 +} to operate the metal ion exchange, to accomplish the molecule modification; a macro molecule combination modification is accomplished through adopting 0.01 percent of cane sugar low molecular polymer, thereby prolonging the half life period of an enzyme preparation, and obviously improving the high temperature resistant ability. The experimental result employed by the enzyme preparation indicates that the nicotine is decreased by 9.3 percent, the total nitrogen is decreased by 5.7 percent, the protein is decreased by 7.1 percent; cigarette smoke condensates are decreased by 8.4 percent, the tar content is decreased by 5.1 percent, cigarette smoke nicotine content is decreased by 28.0 percent, and the carbon is decreased monoxide by 1.6 percent. The enzyme preparation is employed when the tobacco is wet for the second time before defolat and redrying.

The invention discloses a method for preparing t-carrageenase. A seed medium is inoculated with a strain Pseudoalteromonas carrageenovora ASY5 for producing carrageenase, the strain is transferred into a fermentation medium in an inoculation mode for induced enzyme production after activation culture, and t-carrageenase containing fermentation liquor is separated to obtain the t-carrageenase. T-carrageenan is adopted as an inductive agent for fermentation production of the t-carrageenase, a fermentation production technology of the t-carrageenase is established, and the method has good application value for industrial production of carrageenan degrading enzymes and carrageenan oligosaccharide.

The invention is an enzyme, and its characteristic: it can degrade k-carrageenan to prepare oligo-carrageenan and degrade beta-1, 4-indican bond of k-carrageenan, therefore called k-carrageenan degrading enzyme, its molecular weight is 30,000Da. When preparing it, using 2216E culture medium to make shaking bottle culture on the sea Cytophaga sp at 28-35deg.C, then centrifugating the culture solution to obtain fermentation enzyme solution, ultrafiltering and concentrating the enzyme solution, then using 40-80% (NH4)2SO4 for salting-out and collecting protein deposit, and freeze-drying. It can effectively degrade k-carrageenan, be used to prepare oligo-carrageenan, and compared with chemical method, it has simple preparing coursee, high product yield, stable quality, and ensures the activity research and development of oligosaccharide, etc.

The invention belongs to the technical field of biology, and discloses an AFB1 degrading bacterium and an AFB1 degrading enzyme. The degrading bacterium is aspergillusoryzane AOYIN2012Y8 with a collection number of CGMCC No.5817. The aspergillusoryzane AOYIN2012Y8 CGMCC No.5817 is inoculated into a PDA solid culture medium, and constant-temperature culturing is carried out under a temperature of 28-30 DEG C; collection is carried out when a lot of spores grows. The spores are scraped off by using an inoculation loop. The spore concentration is regulated to 1.0*10<8>CFU/mL by using normal saline containing 0.5-0.7% Tween 80. 5-7mL of the spore is inoculated to every 30g of a solid fermentation culture medium. Constant-temperature culturing is carried out for 5-7 days under a temperature of 28-30 DEG C, and collection is carried out. The solid fermentation culture medium is well mixed with normal saline, and the mixture is subjected to room-temperature soaking. The mixture is filtered and centrifuged; a supernatant is fetched and is subjected to salting-out, dialysis, and gel chromatographic separation; and concentrating and lyophilizing are carried out, such that the AFB1 degrading enzyme is obtained. The degrading enzyme provided by the invention has relatively high decomposing capability against AFB1, wherein a maximal degradation rate reaches 80.12%.

The present invention relates to a method for producing a soluble cocoa product from cocoa powder comprising the steps: a) preparing an aqueous suspension of cocoa powder (1), b) treating said suspension with one or more degrading enzymes (2), c) submitting (3) the suspension obtained in step b) to a pH treatment comprising treating said suspension for at least 2 hours at a suitable pH, a temperature of at 10 least 100° C., and a pressure which is at least 1 bar higher than the ambient pressure, d) optionally bringing the pH of the suspension obtained in step c) to a pH value corresponding with the pH of the suspension obtained in step a), e) treating (4) the suspension obtained in step c) or d) with one or more degrading enzymes, f) separating (6) the suspension (5) obtained in step e) into insoluble material (8) and a soluble part (7), and g) obtaining soluble cocoa components (10) from the soluble parts (7). The present invention further relates to cocoa products obtained by the present method and use thereof.

The invention belongs to microbe technical field, and especially relates to a Trichodermaviride, and the invention also relates to an application of Trichodermaviride. The Trichodermaviride strain capable of producing cellulase with high-yield is Trichodermaviride in Trichoderma, the code number is M20, and the Trichodermaviride is preserved at China General Microbiological Culture Collection Center on 2nd, April, 2011, the preservation number is CGMCC No.4732. The strain of the invention can be grown in a medium which takes a plurality of cellulose compounds as a sole carbon source, the cellulose compounds comprise carboxymethylcellulose sodium, microcrystalline cellulose, corn straw, cob, deciduous leaf, turf grass and the like. The Trichodermaviride of the invention can secrete a plurality of cellulose hydrolases, can be used for processing urban landscaping waste, can acquire high reducing sugar in short time, the obtained reducing sugar can be used for producing single cell protein and can be used for producing fuel ethanol, and the Trichodermaviride possesses important meaning for solving a series of problems of energy shortage and environmental pollution at present.

The invention discloses a zearalenone (ZEN) toxin degrading enzyme for acinetobacter and a coding gene and applications of the ZEN toxin degrading enzyme. The amino acid sequence of the ZEN toxin degrading enzyme for the acinetobacter is shown by SEQ ID NO.6. The sequence of the coding gene of the ZEN toxin degrading enzyme for the acinetobacter is shown by SEQ ID NO.5. The ZEN toxin degrading enzyme for the acinetobacter performs stronger degrading capacity on mycotoxin ZEN, is capable of degrading at least 90 percent of ZEN into a low-estrogen active product in 12h without generating high-estrogen active analogues, such as ZEN, zeranol and the like, and has a real detoxification effect. According to the invention, the coding sequence of the ZEN toxin degrading enzyme for acinetobacter sp.SM04 is determined, i.e. a Prx gene, and thereby, a foundation is laid for researching the active site of the enzyme and changing the Prx enzyme activity through site-specific mutagenesis in the future.

The invention provides stenotrophomonassp. H-4 and a preparation method of degrading enzyme preparation thereof as well as application. The stenotrophomonassp. H-4 has been conserved in the conservation unit specified by the State Intellectual Property Office; the conservation date is February 1, 2013; the name of the conservation unit is China General Microbiological Culture Collection Center; and the conservation number is CGMCC No.7274. The stenotrophomonassp. H-4 can be grown on a culture medium with chlorothalonil as the unique carbon source and energy, and after being cultivated for 7 days, the degradation rate of the stenotrophomonassp. H-4 reaches 83.6%; the enzyme activity is 3.28 U and the degradation rate of the degrading enzyme to the chlorothalonil is 86% when the extraction conditions for crude enzyme in the stenotrophomonassp. H-4 are as follows: ultrasonic powder: 30 W; and ultrasonic time: 5 minutes. Obviously, the degrading enzyme preparation provided by the invention has bright application prospect in clearing away residual pesticides on vegetables and fruits.

The invention discloses a lecanicillium psalliotae strain with the preservation number of CGMCC NO: 5329. The strain can colonise spawns, two-year-old larvae and female adults at different life stages of rootknot nematode. Activations of chitinous substance degradative enzyme and excisionenzyme generated by the strain can be respectively determined through an N-acetyl glucosamine method and a p-nitrobenzene method, the enzymatic activities thereof can reach 3.98 U/h.mL and 0.38 U/h.mL, and higher activity of the produced chitinous substance enzyme can be detected at different temperature (27 DEG C-75 DEG C) and different pH (3.0-7.0); and the influence of chitinous substance enzyme extract produced by the strain on eclosion of rootknot nematode spawns is determined in vitro, and the inhibition ratio and the destruction ratio of enzyme solution on relative eclosion of the spawns are respectively 94.52 percent and 84.32 percent. The lecanicillium psalliotae strain disclosed by the invention is applied to biological control of crop rootknot nematodiasis, provides an effective way for protecting sweet potatoes, fruits, vegetables in north China and particularly controlling destructiverootknot nematodiasis, and has obvious ecological benefit, economic value and social value.

The invention relates to the technical field of gene engineering, and particularly provides a zearalenone toxin degrading enzyme mutant and a production strain thereof. Compared with a wild type, mutants H1-T1 and H1-T2 are subjected to recombinant expression in pichia pastoris, the specific enzyme activity is improved by 75.1% and 172.2% respectively, the production cost of the enzyme is reduced, and application and popularization of the enzyme in the field of feed are promoted.

The present invention comprises methods and compositions for the reduction of oxalate in humans, and methods for the purification and isolation of recombinant oxalate reducing enzyme proteins. The invention provides methods and compositions for the delivery of oxalate-reducing enzymes in particle compositions. The compositions of the present invention are suitable in methods of treatment or prevention of oxalate-related conditions.

Provided herein are methods, kits and compositions useful in detecting degradative enzymes and biomolecules in bodily fluid samples.