272 results about "Degradative enzyme" patented technology

The invention discloses a fusion enzyme capable of efficiently degrading PET (polyethylene terephthalate) plastic and application of the fusion enzyme. The invention provides a recombinant fusion enzyme. The recombinant fusion enzyme comprises a mutant KL-MHETase of thermophilic carboxylesterase Est30 and a PET (Polyethylene Terephthalate) degrading enzyme FAST-PETase, the amino acid sequence of the mutant KL-MHETase of the thermophilic carboxylesterase Est30 is as shown in SEQ ID NO. 3; the amino acid sequence of the FAST-PETase is as shown in the 284th to 546th amino acids of SEQ ID NO.1 (Sequence Identifier Number 1). The fusion enzyme is used as a biocatalyst, the activity of catalyzing hydrolysis of MHET at 50 DEG C is 52.6 times that of FAST-PETase, and the fusion enzyme has high enzyme activity and thermal stability. PET degradation is catalyzed at the temperature of 50 DEG C, the conversion rate of PET is 90% after 48 hours of reaction, and meanwhile, the TPA content in the product is close to 100%.

The invention discloses application of fucoidin degrading enzyme OUC-MgFucD1 in preparation of fucoidin oligosaccharide, and belongs to the technical field of preparation of the fucoidin oligosaccharide. The amino acid sequence of the fucoidin degrading enzyme OUC-MgFucD1 is as shown in SEQ ID NO. 1, and the fucoidin degrading enzyme OUC-MgFucD1 is as shown The fucose oligosaccharide is fucose, fucodisaccharide and fucotriose, the molecular formula of the fucose is C6H12O8S, the molecular formula of the fucodisaccharide is C12H22O15S2, and the molecular formula of the fucotriose is C18H32O16S. The invention further discloses a preparation method of the fucose oligosaccharide. The fucoidin degrading enzyme OUC-MgFucD1 disclosed by the invention can specifically act on fucoidin, and a specific fucoidin oligosaccharide combination is generated by precisely cutting a main chain glucosidic bond of the fucoidin. The invention provides a key technical support for the development of high-added-value fucoidan oligosaccharide products, and has important significance for promoting the industrial application of the fucoidan oligosaccharide in the fields of functional foods, biological medicines and the like.

The invention discloses an acid-resistant PET plastic degrading enzyme PETase mutant and application thereof, and belongs to the technical field of biological enzyme engineering. According to the invention, amino acid sequence truncation, single point mutation and pairwise compound mutation are carried out on the basis of wild type FAST-PETase, and the obtained mutant R84D reduces the optimal reaction pH of the FAST-PETase and improves the stability of the FAST-PETase under different pH values. Compared with the enzyme activity of starting FAST-PETase, the enzyme activity of the mutant constructed by the invention is improved by 2.2 times. When the pH value is 6.5-8.5, the stability of R84D is relatively high, and the enzyme activity residue is gt; 60%.

The invention provides an indoxacarb degrading enzyme as well as a coding gene, a production strain and application thereof. Wherein the amino acid sequence of the indoxacarb degrading enzyme is as shown in SEQ ID NO. 1, and the sequence of the coding gene is as shown in SEQ ID NO. 2. The production strain is a recombinant engineering strain comprising the coding gene. The application refers to the application of the indoxacarb degrading enzyme in degrading indoxacarb and repairing an indoxacarb polluted environment, and a corresponding method for degrading indoxacarb or repairing the indoxacarb polluted environment. The indoxacarb degrading enzyme has the advantages that the screened indoxacarb degrading enzyme can efficiently and rapidly degrade indoxacarb, can also be used for repairing environments such as water bodies and soil polluted by indoxacarb, and has important application value.

The invention provides an aflatoxin B1 degrading enzyme as well as a preparation method and application thereof, and belongs to the technical field of agricultural biology, and a nucleotide sequence of the aflatoxin B1 degrading enzyme is shown as SEQ ID NO.1. The invention provides an aflatoxin B1 degrading enzyme as well as a preparation method and application thereof. The purpose of efficiently degrading aflatoxin B1 by using a novel degrading enzyme is achieved.

The invention relates to the technical field of microorganisms, in particular to recombinant pichia pastoris for expressing plastic degrading enzyme LCCICCG as well as a construction method and application of the recombinant pichia pastoris. According to the invention, pichia pastoris is taken as a host cell, the coding gene lccICCG of the plastic degrading enzyme is heterologously expressed, and single-copy or multi-copy recombinant pichia pastoris is constructed. By optimizing culture conditions, the expression quantity and biomass of the plastic degrading enzyme protein are remarkably improved; by optimizing a fermentation strategy, high-density fermentation production of the LCCICCG protein in a 5L fermentation tank is realized, and a technical basis is provided for realizing large-scale industrial production of the protein. The improvements not only improve the production efficiency and reduce the cost of industrial production, but also provide powerful support for commercial application of the plastic depolymerases.

The invention belongs to the field of biology, and discloses a polymyxin B specific degrading enzyme and application thereof. The amino acid sequence of the polymyxin B specific degrading enzyme is as shown in SEQ ID NO: 1. The polymyxin B specific degrading enzyme disclosed by some embodiments of the invention can selectively degrade polymyxin B1-1 and polymyxin B3, and does not influence the activity of polymyxin B1 and polymyxin B2, so that the polymyxin B specific degrading enzyme can be used for purification in the preparation process of polymyxin B; the polymyxin can resist acid, alkali and organic solvents, and can efficiently identify and decompose polymyxin B1-1 and B3 components under various conditions. The porous microspheres are used as the solid-phase carrier, so that the surface of the microspheres can accommodate more enzyme ligands, the acid-base resistance and organic solvent resistance of the ligands can be effectively improved, and the polymyxin B purification efficiency can be effectively improved.

The invention discloses a PET hydrolase mutant as well as a preparation method and application thereof, and belongs to the field of enzyme engineering. On the basis of FAST-PETase, in combination with deep learning prediction and enzyme engineering optimization strategies, a plurality of mutants with specific performance advantages are systematically constructed and screened, the method is suitable for application scenes with different temperatures, substrate loads and degradation efficiency requirements, and experimental basis and technical support are provided for constructing a diversified and combinable PET degrading enzyme system. The mutants show diversified advantages in different performance dimensions, for example, the enzyme activity of the K233R mutant is 1.62 times that of FAST-PETase, and the K233R mutant still keeps good thermal stability (1.03 times) after being subjected to heat treatment for 2 hours at the temperature of 50 DEG C; q224K is more remarkably improved in the aspect of thermal stability, and the residual activity after heat treatment is 1.64 times that of FAST-PETase.

The invention provides recombinant escherichia coli for producing chondroitin sulfate A and application of the recombinant escherichia coli, and relates to the technical field of genetic engineering. According to the recombinant escherichia coli disclosed by the invention, chondroitin synthase, chondroitin 4-O-sulfotransferase, N-acetyl glucosamine epimerase, UDP-glucose dehydrogenase and chondroitin sulfate degrading enzyme are overexpressed; wherein the amino acid sequence of the chondroitin sulfate degrading enzyme is as shown in SEQ ID NO. 5. The recombinant escherichia coli is inoculated into a culture medium for fermentation culture, the average molecular weight of the obtained chondroitin sulfate A is 800Da, and the yield is about 12.5 g / L. Therefore, low-molecular-weight chondroitin sulfate A can be obtained and the yield of chondroitin sulfate A can be increased by fermenting the recombinant escherichia coli disclosed by the invention.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of fumonisins degrading enzyme by forming disulfide bonds. The thermal stability of the fumonisins degrading enzyme FumDPS mutants T194V, T194V-P40C / Y173C, T194V-N43C / D83C and T194V-F71C / S117C related to the invention is obviously improved compared with that of the wild type fumonisins degrading enzyme, and the thermal stability of the fumonisins degrading enzyme FumDPS mutants is obviously improved. The fumonisins enzyme is mainly applied to the industries of livestock feed processing, food production and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins degrading enzyme mutant with improved thermal stability is convenient to transport and store, so that the investment of industrial cost can be reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to a zearalenone toxin degrading enzyme variant and application thereof. The amino acid sequence of the zearalenone toxin degrading enzyme is as shown in SEQ ID NO.2, or the zearalenone toxin degrading enzyme with the amino acid sequence as shown in SEQ ID NO.2 is obtained through amino acid mutation. The zearalenone toxin degrading enzyme mutant is obtained through mutation screening, and compared with a wild type, the zearalenone toxin degrading enzyme mutant has the advantages that the zearalenone degrading capability is obviously improved; besides, the zearalenone toxin degrading enzyme is expressed by using alfalfa, the zearalenone toxin degrading enzyme with biological activity is easy to obtain, and the zearalenone toxin degrading enzyme has certain application potential in prevention and treatment of animal poisoning caused by zearalenone toxin pollution in agriculture and animal husbandry production.

The invention belongs to the technical field of microbial resource development and straw biological decomposition. The invention discloses cold-resistant pseudoarthrobacter psychrotolans CKON-4, and the preservation number of the cold-resistant pseudoarthrobacter psychrotolans CKON-4 is CGMCC (China General Microbiological Culture Collection Center) No.29378. The cold-resistant pseudoarthrobacter psychrotolans CKON-4 has the advantages that the preservation number The strain CKON-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 25, 2023, the China General Microbiological Culture Collection Center is called CGMCC for short, the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number of the strain is CGMCC No.29378. The strain disclosed by the invention has very high lignocellulose degradation activity, and the decomposition rate of straws can be remarkably improved; the strain is separated from rice field soil, can grow in the pH value range of 5-9 and the temperature range of 4-37 DEG C, can tolerate the salt concentration of 10%, and is a bacterium with wide pH application range, salt resistance and cold resistance. The strain can play an important role in lignocellulose degrading enzyme production, straw biological decomposition and compost fermentation.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a fumonisins degrading enzyme mutant with improved specific activity and thermal stability and application. According to the mutants of the fumonisins degrading enzyme FumDSB, which are provided by the invention, the relative activity and the thermal stability of the mutants A373R, A373R / S248P, A373R / Q259P, A373R / T282D, A373R / G321A, A373R / D330P and A373R / A378K are obviously improved compared with those of a wild type fumonisins degrading enzyme. The enzyme is mainly applied to the industries of agricultural products, animal feed, food and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins mutant with improved activity and thermal stability is beneficial to reducing the cost of the enzyme in industrial application.

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention discloses a bisphenol F degradation gene cluster bpf and application thereof. The strain DN12 is separated and can degrade the BPF and grow by taking the BPF as the unique carbon source and energy. On the basis, a BPF degradation gene cluster bpf is obtained from the strain DN12 by adopting whole genome and comparative transcriptome sequencing analysis. Three different degrading enzymes (two-component oxidase BpfAB, monooxygenase BpfC and hydrolase BpfD) are found in the gene cluster and can catalyze gradual degradation of BPF, and a simple aromatic ring compound p-hydroxybenzoic acid is generated.

The invention relates to the technical field of biology, and discloses a novel polyurethane plastic degrading enzyme and application thereof. The amino acid sequence of the novel polyurethane plastic degrading enzyme MBX1 provided by the invention is as shown in SEQ ID No. 1; the sequence of the coding gene is as shown in SEQ ID No.2. Escherichia coli engineering bacteria constructed by using the coding gene sequence can efficiently express the novel polyurethane plastic degrading enzyme. The method specifically comprises the following steps: cloning an MBX1 gene; the preparation method comprises the following steps: constructing a pET28a-MBX1 expression vector; expression, purification and functional verification of MBX1 protein; the degradation rate of the novel polyurethane plastic degrading enzyme to polyether polyol type polyurethane within 8 hours at the temperature of 50 DEG C and the pH value of 7.5 is 98%. The novel polyurethane plastic degrading enzyme can be applied to biological treatment and related research and development of polyurethane plastics, and has a huge application prospect in biodegradation of the polyurethane plastics.

The invention discloses a genetically engineered bacterium for high-yield production of PET degrading enzyme by taking PET hydrolysate as a raw material and application of the genetically engineered bacterium, and belongs to the technical field of microbial engineering. The invention provides a plasmid containing an EG metabolism and degrading enzyme synthesis expression unit, so that a host cell has the capability of metabolizing EG to synthesize degrading enzyme. The constructed recombinant plasmid can be used in common hosts such as escherichia coli and bacillus subtilis, the constructed recombinant strain can be used as a cell catalyst for metabolism of EG and synthesis of degrading enzyme, a new method is provided for recycling of PET, and the method has a wide application prospect.

The invention relates to the field of microorganisms, and discloses a P.megaterium and an application thereof in pig farm manure treatment, the P.megaterium is preserved in China Center for Type Culture Collection on December 13, 2022, the preservation address is Wuhan, China, the classification name of the P.megaterium is Pristia megaterium 3144, and the preservation number is CCTCC NO: M20221962. The content of pollutants such as total nitrogen, chemical oxygen demand and the like in pig farm feces is reduced by secreting various degrading enzymes such as protease, lipase and the like through the provided Priestia megaterium 3144 strain, and meanwhile, fecal coliforms and roundworm eggs are killed by the strain through metabolism heat production and antibacterial substance secretion.

The invention relates to the technical field of bioengineering, in particular to recombinant saccharomyces cerevisiae for producing a human epidermal growth factor based on a biological membrane as well as a construction method and application of the recombinant saccharomyces cerevisiae. According to the invention, saccharomyces cerevisiae BY4742 and 530-1C are used as chassis cells for expressing the human epidermal growth factor (hEGF), and the yield of the human epidermal growth factor (hEGF) is increased to 15 mg / L from 80 [mu] g / L by means of gene modification, promoter optimization, signal peptide optimization and the like; by optimizing related modules in an engineering protein secretion pathway, single or combined deletion of degrading enzyme genes (PEP4, PRB1 and YAP3) and overexpression of key node genes (KAR2, PDI1, HAC1, ERV29, ERO1, SRP54, SEC24 and DER1) in the protein secretion pathway, the hEGF yield is increased to the level of 141 mg / L; a biological membrane strain with the performance of flocculation and adhesion to an abiotic surface is constructed by selecting an endogenous flocculation gene FLO11 of overexpression yeast and an exogenous gene ALS3 of tritirachium album, and continuous production of hEGF is realized based on biological membrane immobilized continuous fermentation, and the yield is stabilized at 300 mg / L or above.

The invention discloses a PET plastic degrading enzyme LCC mutant as well as a protein crystal and application thereof, and relates to the technical field of biology, the LCC mutant is obtained by modification on the basis of a wild PET plastic degrading enzyme LCC, and the amino acid sequence of the LCC mutant is shown as SEQ ID NO.1. Compared with a wild type LCC, the LCC mutant has higher thermal stability, the Tm value of the LCC mutant is 91.30 DEG C, the protein Tm value of the wild type LCC is 86.76 DEG C and is increased by 4.54 DEG C compared with that of the wild type LCC, good activity is still kept, and the yield of the mutant LCC-M4 is 2 times higher than that of the wild type LCC. In addition, the invention further provides a protein crystal and a structure of the LCC mutant, and a research basis is provided for further modifying the PET plastic degrading enzyme LCC with high activity and high stability.

The invention discloses a dsPETase01 plastic degrading enzyme mutant and application thereof, and relates to the technical field of bioengineering, the amino acid sequence of a wild type plastic degrading enzyme (dsPETase01) is designed and modified, 8 dsPETase01 single mutation sites or 19 combined mutation sites are provided, the thermal stability of the obtained dsPETase01 mutant is improved by about 4-21 DEG C compared with that of the wild type dsPETase01, and the dsPETase01 mutant can be used for preparing a plastic degrading enzyme. And the activity is improved by nearly 1.2-3 times. The invention also provides four preferable mutant proteins which have higher yield, activity and thermal stability compared with wild dsPETase01, have wider application conditions, are more suitable for degrading PET (Polyethylene Terephthalate) plastics, and are beneficial to large-scale production and industrial application.

The invention provides a stem cell exosome for overexpressing A beta degrading enzyme as well as a preparation method and application of the stem cell exosome. Specifically, the invention provides an application of the stem cell exosome overexpressing A beta degrading enzyme in preparation of drugs, and the drugs are used for at least one of the following: prevention, improvement or treatment of cognitive impairment related diseases; neuronal cell damage is improved; wherein the stem cells for overexpressing the A beta degrading enzyme are prepared through lentivirus transfection. According to the stem cell exosome overexpressing the A beta degrading enzyme, the content of the A beta degrading enzyme is obviously higher than that of the exosome prepared by a traditional method. Therefore, when the compound is prepared into a medicine for treating or preventing cognitive impairment related diseases, the medicine can be efficiently conveyed to the brain, and the concentration of the medicine in the brain is remarkably improved, so that the curative effect is enhanced, the dosage of the medicine can be reduced, the side effect is reduced, and the prognosis of brain diseases is effectively improved.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of fumonisins degrading enzyme by forming disulfide bonds. The thermal stability of the fumonisins degrading enzyme FumD mutant provided by the invention is obviously improved compared with that of wild type fumonisins degrading enzyme. The fumonisins enzyme is mainly applied to the industries of livestock feed processing, food production and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins degrading enzyme mutant with improved thermal stability is beneficial to keeping the activity of the enzyme at a higher temperature, adapts to a higher-temperature production environment, reduces the use amount and replacement frequency of the enzyme, and is convenient to transport and store due to the improvement of the stability, so that the investment of industrial cost can be reduced.

The invention discloses a biodegradable light-package high-barrier packaging film and a preparation process, and relates to the technical field of packaging films, the biodegradable light-package high-barrier packaging film comprises the following components by weight: 8-15 parts of modified montmorillonite, 30-50 parts of modified nano cellulose, 5-10 parts of polyvinyl alcohol, 1.5-3 parts of a plasticizer, 0.25-0.6 part of curcumin, and 40-60 parts of deionized water. According to the biodegradable light-package high-barrier packaging film and the preparation process, montmorillonite is modified, so that the montmorillonite can interact with anions on a bacterial cell membrane, the integrity of the cell membrane is destroyed, and bacterial contents are leaked, thereby inhibiting growth and reproduction of bacteria and improving the antibacterial property of the packaging film; montmorillonite is a natural mineral material, is environment-friendly, and can change the crystal structure of polyvinyl alcohol, so that the degrading enzyme is easier to contact with a polymer molecular chain, and the degradation speed is accelerated.

The invention discloses an IsPETase mutant with improved thermal stability and activity and application of the IsPETase mutant. According to the invention, a mutant TS-PETase of PET plastic degrading enzyme IsPETase is taken as a parent, and the IsPETase mutant with improved thermal stability and / or PET degrading activity is obtained by performing single-point mutation or combined mutation on amino acid at a specific site of the mutant TS-PETase. The obtained IsPETase mutant can be used for effectively degrading PET (Polyethylene Terephthalate) plastics under a Tg condition, and can also be prepared into a corresponding preparation for degrading the PET plastics accumulated in the environment and improving the environment polluted by the PET plastics. The obtained IsPETase mutant can also be used for degrading PET-containing plastic products and assisting in harmless treatment of plastics. The IsPETase mutant with improved thermal stability and activity, provided by the invention, has a promoting effect on the development of industrial biodegradation of PET (Polyethylene Terephthalate) plastics.

The invention relates to salt-tolerant paracoccus and application thereof in wastewater treatment, and belongs to the technical field of environmental microorganisms. The Paracoccus sp. S16 is preserved in the China General Microbiological Culture Collection Center on May 28, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.34704. The strain has unique salt tolerance and various degrading enzyme systems, and can be applied to the field of biological engineering, biological engineering and the like. The catalyst can effectively degrade petroleum hydrocarbons, polymers and other organic pollutants with high toxicity in drilling fluid wastewater, has wide environmental adaptability, and has great application potential.

The invention discloses low-glycemic-index starch and a processing method thereof, and belongs to the field of starch modification processing. The preparation method comprises the following steps: adding a proper amount of deionized water into starch to prepare a starch emulsion, uniformly stirring, regulating the pH value to 6.0 by using a citric acid buffer solution, carrying out microwave treatment on the starch emulsion under the condition that the power is 700-900W, regulating the pH value to 6.5 by using the citric acid buffer solution, adding 40000U / g of high-temperature-resistant alpha-amylase, carrying out enzymolysis, and carrying out high-pressure treatment; adjusting the pH value to 5.0 by using a citric acid buffer solution, adding 8U / mL of pullulanase, reacting, and degrading enzyme activity in a high-temperature sterilization pot; and standing, naturally cooling, carrying out low-temperature aging treatment, centrifuging, removing supernatant, dehydrating, drying, crushing and sieving to obtain the low-glycemic-index starch. The influence of starch aging on the food GI index can be fully utilized, and the GI index of the product is reduced. And the production process is simple, low in cost and low in energy consumption. The starch has the characteristics of high resistant starch content and low glycemic index.

The present invention discloses the use of 3,4-dihydropsoralen in the preparation of a drug for preventing or treating rheumatoid arthritis. The present invention simulates RA by constructing TNF-a-induced SW982 cells. The results show that 3,4-dihydropsoralen (DP) can downregulate the mRNA expression of inflammatory factors, chemokines, and matrix degrading enzymes through the IL-17 signaling pathway, and affect the IL-17 signaling pathway and the NF-κB signaling pathway to jointly exert an anti-inflammatory effect, thereby effectively improving rheumatoid arthritis. This provides a theoretical basis for the application of 3,4-dihydropsoralen in the clinical treatment of rheumatoid arthritis and opens up new therapeutic drugs and approaches.

The invention discloses recombinant bacillus subtilis capable of efficiently expressing a plastic degrading enzyme LCCICCG as well as a construction method and application of the recombinant bacillus subtilis. The recombinant bacillus subtilis is used for overexpressing the plastic degrading enzyme LCCICCG and signal peptide. By constructing plasmids of different signal peptides and promoters, the biomass of the recombinant bacillus subtilis and the protein expression quantity of the LCCICCG in the fermentation culture process are improved, and the industrial production cost of the LCCICCG is reduced.

The invention belongs to the technical field of microbiology and enzyme engineering, and relates to a PET degrading strain derived from deep sea, a PET degrading enzyme and application thereof. The invention discloses a PET (Polyethylene Terephthalate) degrading strain derived from deep sea, which is halophilic pseudomonas sp. EEDS01, is preserved in China Center for Type Culture Collection on November 28, 2024, and has the preservation number of CCTCC NO: M20242659. The invention further discloses a preparation method of the PET degrading strain. The invention also provides PET (Polyethylene Terephthalate) degrading enzymes PnPETase1, PnPETase2, PnPETase3 and PnPETase4 which are derived from the bacterial strain, and the amino acid sequences of the PET degrading enzymes are respectively shown as SEQ ID NO.2-5 in a sequence table. The bacterial strain for degrading PET and four PET degrading enzymes provided by the invention have strong PET hydrolysis capability under high salt concentration, and have important significance in the fields of environmental protection and catalysis.