170 results about "Degradative enzyme" patented technology

The invention belongs to the technical field of bioengineering, and particularly relates to a zearalenone toxin degrading enzyme variant and application thereof. The amino acid sequence of the zearalenone toxin degrading enzyme is as shown in SEQ ID NO.2, or the zearalenone toxin degrading enzyme with the amino acid sequence as shown in SEQ ID NO.2 is obtained through amino acid mutation. The zearalenone toxin degrading enzyme mutant is obtained through mutation screening, and compared with a wild type, the zearalenone toxin degrading enzyme mutant has the advantages that the zearalenone degrading capability is obviously improved; besides, the zearalenone toxin degrading enzyme is expressed by using alfalfa, the zearalenone toxin degrading enzyme with biological activity is easy to obtain, and the zearalenone toxin degrading enzyme has certain application potential in prevention and treatment of animal poisoning caused by zearalenone toxin pollution in agriculture and animal husbandry production.

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention relates to the field of microorganisms, and discloses a P.megaterium and an application thereof in pig farm manure treatment, the P.megaterium is preserved in China Center for Type Culture Collection on December 13, 2022, the preservation address is Wuhan, China, the classification name of the P.megaterium is Pristia megaterium 3144, and the preservation number is CCTCC NO: M20221962. The content of pollutants such as total nitrogen, chemical oxygen demand and the like in pig farm feces is reduced by secreting various degrading enzymes such as protease, lipase and the like through the provided Priestia megaterium 3144 strain, and meanwhile, fecal coliforms and roundworm eggs are killed by the strain through metabolism heat production and antibacterial substance secretion.

The invention provides a stem cell exosome for overexpressing A beta degrading enzyme as well as a preparation method and application of the stem cell exosome. Specifically, the invention provides an application of the stem cell exosome overexpressing A beta degrading enzyme in preparation of drugs, and the drugs are used for at least one of the following: prevention, improvement or treatment of cognitive impairment related diseases; neuronal cell damage is improved; wherein the stem cells for overexpressing the A beta degrading enzyme are prepared through lentivirus transfection. According to the stem cell exosome overexpressing the A beta degrading enzyme, the content of the A beta degrading enzyme is obviously higher than that of the exosome prepared by a traditional method. Therefore, when the compound is prepared into a medicine for treating or preventing cognitive impairment related diseases, the medicine can be efficiently conveyed to the brain, and the concentration of the medicine in the brain is remarkably improved, so that the curative effect is enhanced, the dosage of the medicine can be reduced, the side effect is reduced, and the prognosis of brain diseases is effectively improved.

The invention relates to salt-tolerant paracoccus and application thereof in wastewater treatment, and belongs to the technical field of environmental microorganisms. The Paracoccus sp. S16 is preserved in the China General Microbiological Culture Collection Center on May 28, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.34704. The strain has unique salt tolerance and various degrading enzyme systems, and can be applied to the field of biological engineering, biological engineering and the like. The catalyst can effectively degrade petroleum hydrocarbons, polymers and other organic pollutants with high toxicity in drilling fluid wastewater, has wide environmental adaptability, and has great application potential.

The invention provides a beautifying composition and application thereof in whitening, anti-inflammatory and anti-aging products, and belongs to the technical field of cosmetics. The beautifying composition is prepared from collagen peptide, sodium hyaluronate and ergothioneine, wherein the mass concentration ratio of the collagen peptide to the sodium hyaluronate to the ergothioneine is (75 to 85) to (3.5 to 10) to (0.4 to 3.5); the beautifying composition shows remarkable advantages in the aspects of collagen regeneration and skin improvement, the collagen content can be remarkably increased, and multi-effect collagen regeneration promotion is achieved by inhibiting collagen degrading enzyme MMP-1 and up-regulating TGF-beta1; the content of AQP3 and HA can be increased, and water locking and moisturizing of the skin are enhanced; by activating an Nrf2 pathway, the SOD2 level is improved, IL-6 and IL-1alpha are inhibited, and the antioxidant, anti-inflammatory and whitening effects are achieved; the thickness of an epidermal layer and the density of dermal fibroblasts can be remarkably improved, wrinkle resistance and repairing are effectively achieved, and the skin structure is improved.

The invention discloses polyethylene glycol terephthalate hydrolase PmPETase and application thereof.The hydrolase PmPETase is protein with the amino acid sequence shown in SEQ ID NO.1. An expression system suitable for the PmPETase is constructed, recombinase protein with catalytic activity is obtained, and the polyethylene glycol terephthalate hydrolase PmPETase can be used for preparing the polyethylene glycol terephthalate hydrolase PmPETase. The substrate specificity, the catalytic property and the potential plastic degradation capacity of the PET degrading enzyme are comprehensively analyzed, and a new theoretical basis and application reference are provided for development and utilization of the PET degrading enzyme and a high polymer material green treatment technology.

The invention discloses a tenuilia microspora NB1-3, a leavening agent and application of the tenuilia microspora NB1-3 in preparation of caragana microspora and moringa oleifera fermented protein feed. The strain is classified and named as nicotiana microspora, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251872. The core of the method is that crude fibers and antinutritional factors (tannin and phytic acid) in moringa oleifera and caragana microspora are efficiently degraded by utilizing a strong lignocellulose degrading enzyme system of the tenuilia microspora NB1-3, and meanwhile, the crude protein content of the feed is increased. The prepared fermented protein feed is high in crude protein content and low in crude fiber content and meets the protein feed standard, efficient and high-value utilization of woody plant resources such as caragana microphylla and moringa oleifera is achieved, and a new high-quality and economical protein feed source is provided for animal husbandry.

This invention relates to the field of enzyme engineering technology, and in particular to a highly efficient lactone hydrolase mutant for degrading zearalenone and its applications. This lactone hydrolase mutant uses the lactone hydrolase gene from Monosporascus sp. GIB2 as a template, and performs a site-directed mutation at amino acid position 134 to obtain L134A, L134V, L134I, and L134M mutants. Under optimal conditions of pH 9.0 and 60℃, these single-point mutants can efficiently degrade zearalenone (ZEN) within 3 minutes. The enzyme activity for degrading zearalenone is significantly improved compared to the original enzyme, with degradation activities increasing by 1.22 (L134A), 1.25 (L134V), 1.17 (L134I), and 1.14 (L134M), respectively, demonstrating significant applications and economic value in the feed industry.

The present application relates to the field of genetic engineering and PET material degradation, and particularly relates to a PET degrading enzyme mutant and its application in PET material degradation. The present application improves the activity and heat resistance of PET degrading enzyme MuPETase derived from Mumia sp., and screens to obtain mutants Mut1-3, Mut5-8 which show good degradation capacity to PET material under acidic conditions, and the amino acid sequences are shown in SEQ ID NO. 3-5, SEQ ID NO. 7-10 respectively. Compared with the original PET degrading enzyme, the heat stability and / or specific activity of the obtained mutant are significantly improved, and the degradation effect on PET material is greatly improved. The obtained mutant has important application value in the biological degradation treatment of single and mixed PET material waste.

The invention relates to the technical field of microorganisms, in particular to a saline-alkaline-tolerant bacillus velezensis JT6, a cellulose degrading enzyme secreted by the same and application of the saline-alkaline-tolerant bacillus velezensis JT6. The bacillus velezensis JT6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 5, 2025, and the preservation number is CGMCC No.36918. The bacillus velezensis JT6 disclosed by the invention can be used for preparing the bacillus velezensis JT6. The strain has cellulose degradation activity, the degradation rate of corn straw is 48.8%, and the degradation rate of rice straw is 24.92%. The strain provided by the invention is used for rapid decomposition of straw returning, is safe to human and livestock, has no pollution to the environment, and has good development and application prospects.

The invention discloses a modified bio-enzyme preparation and application thereof in synergistic microbial pretreatment production of sticky paper pulp, and belongs to the technical field of paper pulp manufacturing. The modified biological enzyme preparation is prepared from cellulase, hemicellulase, lignin degrading enzyme, hydrophobic amino acid and a surfactant. The modified bio-enzyme preparation cooperates with microorganisms to pre-treat wood pulp fibers, and pulping treatment of a double-screw pulping machine is combined, so that the pulping efficiency can be greatly improved, the pulping energy consumption can be reduced, the prepared sticky paper pulp is high in strength and good in performance, and the tensile index and burst index of paper are improved. The method has the characteristics of energy conservation, environmental protection and high efficiency, responds to a new concept of high-valued sustainable development of biomass, and has high popularization value.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

The present application relates to the field of bioengineering, and particularly relates to a PET plastic degrading enzyme mutant, an expression vector, a host and application thereof. The present application provides application of a mutation site as a target in improving the enzyme activity and / or thermal stability of a PETase hydrolytic enzyme; the mutation site includes one or more of positions 114, 175, 205, 234, 269 and 233 in the amino acid sequence of a wild-type PETase hydrolytic enzyme. The present application uses the method of semi-rational design and error-prone PCR to screen and verify sites that have not been mined by other researchers, and expects to further improve the activity and thermal stability of the enzyme to meet the needs of industrial applications.

The invention relates to cutinase as well as a coding gene and application thereof. The amino acid sequence of the cutinase SCC is as shown in SEQ ID NO. 1, and the nucleotide sequence of the cutinase SCC is as shown in SEQ ID NO. 2. The inventor of the application finds out a novel cutinase SCC of a serine hydrolase family from Saccharothrix carnea, and the cutinase SCC is easy for heterologous expression and purification, and can efficiently and specifically catalyze and degrade mono (2-hydroxyethyl) terephthalate (MHET) and polyethylene glycol terephthalate (PET). And compared with the existing MHET degrading enzyme, the MHET degrading enzyme has the advantages that the catalytic efficiency is obviously improved, the use amount of the enzyme can be effectively reduced in the process of catalytically degrading MHET and PET, and the use cost of the enzyme is reduced.

The invention belongs to the technical field of biology, relates to a zearalenone degrading enzyme, and particularly relates to a bacillus subtilis source zearalenone degrading enzyme, an engineering strain thereof and an enzyme preparation prepared from the bacillus subtilis source zearalenone degrading enzyme. Compared with the known zearalenone degrading enzyme, the zearalenone degrading enzyme not only has obvious structure and sequence differences, but also shows an excellent degrading effect at low concentration. The lactone hydrolase derived from bacillus is found for the first time, and the biological resource basis of zearalenone degrading enzyme is expanded. The enzyme not only has significant difference from other degrading enzymes in structure, but also shows unique characteristics in degradation mechanism. The capability of efficiently degrading zearalenone shows that the bacillus subtilis has a wide application prospect in the fields of agriculture, food industry and the like. In addition, the invention also covers the structure of a degradation product generated by the zearalenone enzyme, and provides a new perspective for understanding a zearalenone degradation mechanism.

The present application relates to the field of food processing technology, and provides a method and a device for purine reduction, and an equipment and an electronic equipment and a storage medium. The method for purine reduction includes the following steps: first cooling step: performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point. By performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point, the activity of ATP-degrading enzyme in the food material to be treated can be rapidly inhibited, which can quickly and effectively inhibit the generation of hypoxanthine, thereby reducing the generation of hypoxanthine, reducing the risk of hyperuricemia and gout, and having little effect on the taste and quality of the food material.

The invention relates to methods of identifying enzymes and methods of screening for optimised enzymes for degrading polymers such as plastics. The invention also relates to polymer degrading enzymes and nucleic acids encoding these enzymes as well as expression cassettes and host cells comprising the nucleic acids. The invention also relates to uses of these enzymes or the host cells comprising the nucleic acids encoding these enzymes to degrade polymers such as plastics. Lastly, the invention also relates to the crystal structure of plastic degrading enzymes and uses of these structures.

The invention discloses a monooxygenase gene bpfC, an enzyme coded by the monooxygenase gene bpfC and application of the monooxygenase gene bpfC. The strain DN12 is separated and can degrade the BPF and grow by taking the BPF as the unique carbon source and energy. On the basis, a BPF degradation gene cluster bpf is obtained from the strain DN12 by adopting whole genome and comparative transcriptome sequencing analysis. Three different degrading enzymes (two-component oxidase BpfAB, monooxygenase BpfC and hydrolase BpfD) are found in the gene cluster and can catalyze gradual degradation of BPF, and a simple aromatic ring compound p-hydroxybenzoic acid is generated.

The present application relates to a novel PET-degrading enzyme and a use thereof.

The invention belongs to the technical field of genetic engineering, and discloses application of an anchoring protein INP-N in a PET degrading enzyme system displayed on the surface. According to the invention, after efficient anchoring protein INP-N is screened, FAST-PETase is mediated by the efficient anchoring protein INP-N to construct recombinant escherichia coli of which the surface displays PET degrading enzyme. Furthermore, hydrophobin HFBII is introduced into the surface display system, so that the relative enzyme activity of the PET degrading enzyme is improved by 1.1 times or more. Meanwhile, FAST-PETase and carboxylesterase Est30KL are synchronously expressed, so that the proportion of TPA in a degradation product can reach 95% or above. In addition, the PET degrading enzyme surface display system provided by the invention is excellent in cycle performance, more than 65% of activity can still be retained when PET is cyclically degraded for the third time, and the application cost can be greatly reduced. The method can be applied to the fields of degradation of PET products, preparation of PET degradation agents or BHET degradation agents and the like.

The invention belongs to the field of gene engineering, and particularly relates to a PU hydrolase UMG-SP1 mutant with high catalytic activity and application thereof. The amino acids at the 126, 173, 202, 364, 302, 172, 178, 89, 129, 298, 79 and 201 sites in the amino acid sequence of the PU depolymerases as shown in SEQ ID NO: 1 are mutated by using structural analysis and site-specific mutagenesis technologies to obtain a plurality of mutants, and the mutants with improved catalytic activity are obtained. Experiments prove that the PU depolymerization mutants improve the degradation capacity of PU plastic by promoting the hydrolysis efficiency of carbamate bonds in PU, and an important degrading enzyme element is provided for developing an efficient and environment-friendly PU plastic enzymatic degradation technology.

The invention relates to the field of enzyme engineering, in particular to a mutant of nylon degrading enzyme and application of the mutant. The mutant of the nylon degrading enzyme is obtained by mutation at one or more key sites through stability modification guided by machine learning, substrate channel inlet loop region modification and tetramer interface modification on the basis of a wild type nylon degrading enzyme NylCp2-TS as shown in SEQ ID NO: 1; the mutant enzyme can efficiently degrade nylon materials in various forms, including films, particles and industrial fibers of nylon 6 and nylon 66, and has a wide application prospect in the field of nylon waste biodegradation.

The invention discloses a hydrolase gene bpfD, an enzyme coded by the hydrolase gene bpfD and application. The strain DN12 is separated and can degrade the BPF and grow by taking the BPF as the unique carbon source and energy. On the basis, a BPF degradation gene cluster bpf is obtained from the strain DN12 by adopting whole genome and comparative transcriptome sequencing analysis. Three different degrading enzymes (two-component oxidase BpfAB, monooxygenase BpfC and hydrolase BpfD) are found in the gene cluster and can catalyze gradual degradation of BPF, and a simple aromatic ring compound p-hydroxybenzoic acid is generated.

This invention discloses a method for promoting the biodegradation of N,N-dimethylformamide and simultaneous denitrification. The method constructs a zero-valent iron (ZFE)-coupled biological treatment system, which not only accelerates electron transfer efficiency but also significantly increases the activity of DMF-degrading enzymes and nitrate reductases, synergistically promoting electron transfer and microbial metabolic activity, thereby enhancing the removal of DMF and nitrate nitrogen. Compared to biological treatment systems that only add activated sludge without adding ZFE, adding ZFE significantly improves the removal efficiency of DMF and nitrate nitrogen, making it suitable for the treatment of large-scale polluted water bodies or industrial wastewater.

The application discloses a preparation method of xylose, which comprises the following steps: S1, adding xylanase into a xylose mother liquor for enzyme hydrolysis and viscosity reduction; S2, adding calcium hydroxide into the enzyme hydrolysis liquor and passing in CO2 to generate nanometer calcium carbonate floccus to adsorb impurities; S3, performing cross-flow filtration on the ceramic-polyether sulfone composite membrane to improve the purity of the xylose; S4, performing multistage countercurrent chromatography separation on the calcium type strong acid cation resin, and dynamically adjusting an elution gradient through online HPLC feedback; and S5, performing three-effect evaporation concentration, then stage cooling crystallization, adding alpha type xylose crystal seeds to control the crystal form; the application utilizes enzyme hydrolysis-flocculation to cooperatively reduce the viscosity of the mother liquor to greater than or equal to 45%, and the nanometer floccus has an impurity adsorption efficiency greater than or equal to 85%; a dynamic chromatography separation factor is improved to 1.8, and the xylose recovery rate is greater than or equal to 96.2%; alpha type crystal purity greater than or equal to 99.5% is obtained through gradient crystallization, and the comprehensive energy consumption is reduced; and the enzyme hydrolysis conversion of the raffinate generates xylooligosaccharide and high fructose syrup.

The application provides a hot liquid source quorum quenching strain Shiquan Haeyuan bacteria and application thereof, the strain screened can inhibit pathogenic bacteria and is used for preparing bacteriostatic drugs; the preservation number of the Shiquan Haeyuan bacteria screened is CCTCC NO: M 20251224.The application also provides an AHL degradation enzyme Aii937 protein separated from the YESM3 strain, characterized in that the amino acid sequence of the protein is SEQ ID NO: 2.The YESM3 strain of the application can block the QS path of pathogenic bacteria through AHL lactonase Aii937, reduce the secretion of virulence factors of pathogenic bacteria; the strain YESM3 has no pathogenicity.AHL lactonase does not have broad spectrum, has strong substrate specificity, and only degrades C6-HSL and 3OC-C 14 -HSL.

The invention discloses an aflatoxin B1 degrading enzyme and application thereof. The degrading enzyme comprises lignin peroxidase, dye decoloring peroxidase and multifunctional peroxidase. According to the invention, an efficient and targeted aflatoxin B1 degrading enzyme screening model is established by adopting a molecular simulation technology, and the problems of high complexity, low screening efficiency and the like commonly existing in a traditional screening method are solved. The engineering strain provided by the invention takes escherichia coli Shuffle T7-B as a host, has a stable degrading enzyme expression characteristic, and ensures the efficient expression of the degrading enzyme. The degradation rate of the degrading enzyme provided by the invention on aflatoxin B1 reaches 84% or above.

The invention provides a method for improving the molecular weight of polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short) synthesized by microorganisms, and relates to a method for improving the molecular weight of the polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short). By knocking out or knocking down endogenous (intracellular) carboxylesterase, lipase, esterase, thioesterase and PHA degrading enzyme genes of bacteria for synthesizing PHA, the molecular weight of various synthesized PHA is obviously improved. The method has universality for improving the molecular weight of various PHAs synthesized by different species of bacteria, the mechanical property of the PHA can be improved by improving the molecular weight of the PHA, and new performance is provided for expanding the application of the PHA.

The application discloses a method for recovering terephthalic acid from polyester-containing products, which comprises the following steps: (a) performing an enzymatic reaction by contacting the polyester-containing products with a degradation enzyme, using ammonia water / ammonia gas / liquid ammonia / ammonium hydroxide as a pH regulator in the enzymatic reaction, and forming a degradation solution containing ammonium terephthalate and a dihydric alcohol after the enzymatic reaction; (c) performing a heating treatment on the degradation solution; and (d) obtaining solid terephthalic acid through solid-liquid separation. Through the cycle mode of water dissolution-reconcentration-again deamination, the total recovery rate of PTA is significantly improved to more than 90%, efficient utilization of materials is realized, and the whole process still does not need to add acid and alkali, no waste salt is generated, and the process is green and economical.