106 results about "Degradative enzyme" patented technology

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention provides a beautifying composition and application thereof in whitening, anti-inflammatory and anti-aging products, and belongs to the technical field of cosmetics. The beautifying composition is prepared from collagen peptide, sodium hyaluronate and ergothioneine, wherein the mass concentration ratio of the collagen peptide to the sodium hyaluronate to the ergothioneine is (75 to 85) to (3.5 to 10) to (0.4 to 3.5); the beautifying composition shows remarkable advantages in the aspects of collagen regeneration and skin improvement, the collagen content can be remarkably increased, and multi-effect collagen regeneration promotion is achieved by inhibiting collagen degrading enzyme MMP-1 and up-regulating TGF-beta1; the content of AQP3 and HA can be increased, and water locking and moisturizing of the skin are enhanced; by activating an Nrf2 pathway, the SOD2 level is improved, IL-6 and IL-1alpha are inhibited, and the antioxidant, anti-inflammatory and whitening effects are achieved; the thickness of an epidermal layer and the density of dermal fibroblasts can be remarkably improved, wrinkle resistance and repairing are effectively achieved, and the skin structure is improved.

The invention discloses polyethylene glycol terephthalate hydrolase PmPETase and application thereof.The hydrolase PmPETase is protein with the amino acid sequence shown in SEQ ID NO.1. An expression system suitable for the PmPETase is constructed, recombinase protein with catalytic activity is obtained, and the polyethylene glycol terephthalate hydrolase PmPETase can be used for preparing the polyethylene glycol terephthalate hydrolase PmPETase. The substrate specificity, the catalytic property and the potential plastic degradation capacity of the PET degrading enzyme are comprehensively analyzed, and a new theoretical basis and application reference are provided for development and utilization of the PET degrading enzyme and a high polymer material green treatment technology.

The invention discloses a tenuilia microspora NB1-3, a leavening agent and application of the tenuilia microspora NB1-3 in preparation of caragana microspora and moringa oleifera fermented protein feed. The strain is classified and named as nicotiana microspora, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251872. The core of the method is that crude fibers and antinutritional factors (tannin and phytic acid) in moringa oleifera and caragana microspora are efficiently degraded by utilizing a strong lignocellulose degrading enzyme system of the tenuilia microspora NB1-3, and meanwhile, the crude protein content of the feed is increased. The prepared fermented protein feed is high in crude protein content and low in crude fiber content and meets the protein feed standard, efficient and high-value utilization of woody plant resources such as caragana microphylla and moringa oleifera is achieved, and a new high-quality and economical protein feed source is provided for animal husbandry.

This invention relates to the field of enzyme engineering technology, and in particular to a highly efficient lactone hydrolase mutant for degrading zearalenone and its applications. This lactone hydrolase mutant uses the lactone hydrolase gene from Monosporascus sp. GIB2 as a template, and performs a site-directed mutation at amino acid position 134 to obtain L134A, L134V, L134I, and L134M mutants. Under optimal conditions of pH 9.0 and 60℃, these single-point mutants can efficiently degrade zearalenone (ZEN) within 3 minutes. The enzyme activity for degrading zearalenone is significantly improved compared to the original enzyme, with degradation activities increasing by 1.22 (L134A), 1.25 (L134V), 1.17 (L134I), and 1.14 (L134M), respectively, demonstrating significant applications and economic value in the feed industry.

The present application relates to the field of genetic engineering and PET material degradation, and particularly relates to a PET degrading enzyme mutant and its application in PET material degradation. The present application improves the activity and heat resistance of PET degrading enzyme MuPETase derived from Mumia sp., and screens to obtain mutants Mut1-3, Mut5-8 which show good degradation capacity to PET material under acidic conditions, and the amino acid sequences are shown in SEQ ID NO. 3-5, SEQ ID NO. 7-10 respectively. Compared with the original PET degrading enzyme, the heat stability and / or specific activity of the obtained mutant are significantly improved, and the degradation effect on PET material is greatly improved. The obtained mutant has important application value in the biological degradation treatment of single and mixed PET material waste.

The invention relates to the technical field of microorganisms, in particular to a saline-alkaline-tolerant bacillus velezensis JT6, a cellulose degrading enzyme secreted by the same and application of the saline-alkaline-tolerant bacillus velezensis JT6. The bacillus velezensis JT6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 5, 2025, and the preservation number is CGMCC No.36918. The bacillus velezensis JT6 disclosed by the invention can be used for preparing the bacillus velezensis JT6. The strain has cellulose degradation activity, the degradation rate of corn straw is 48.8%, and the degradation rate of rice straw is 24.92%. The strain provided by the invention is used for rapid decomposition of straw returning, is safe to human and livestock, has no pollution to the environment, and has good development and application prospects.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

The present application relates to the field of bioengineering, and particularly relates to a PET plastic degrading enzyme mutant, an expression vector, a host and application thereof. The present application provides application of a mutation site as a target in improving the enzyme activity and / or thermal stability of a PETase hydrolytic enzyme; the mutation site includes one or more of positions 114, 175, 205, 234, 269 and 233 in the amino acid sequence of a wild-type PETase hydrolytic enzyme. The present application uses the method of semi-rational design and error-prone PCR to screen and verify sites that have not been mined by other researchers, and expects to further improve the activity and thermal stability of the enzyme to meet the needs of industrial applications.

The invention belongs to the technical field of biology, relates to a zearalenone degrading enzyme, and particularly relates to a bacillus subtilis source zearalenone degrading enzyme, an engineering strain thereof and an enzyme preparation prepared from the bacillus subtilis source zearalenone degrading enzyme. Compared with the known zearalenone degrading enzyme, the zearalenone degrading enzyme not only has obvious structure and sequence differences, but also shows an excellent degrading effect at low concentration. The lactone hydrolase derived from bacillus is found for the first time, and the biological resource basis of zearalenone degrading enzyme is expanded. The enzyme not only has significant difference from other degrading enzymes in structure, but also shows unique characteristics in degradation mechanism. The capability of efficiently degrading zearalenone shows that the bacillus subtilis has a wide application prospect in the fields of agriculture, food industry and the like. In addition, the invention also covers the structure of a degradation product generated by the zearalenone enzyme, and provides a new perspective for understanding a zearalenone degradation mechanism.

The present application relates to the field of food processing technology, and provides a method and a device for purine reduction, and an equipment and an electronic equipment and a storage medium. The method for purine reduction includes the following steps: first cooling step: performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point. By performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point, the activity of ATP-degrading enzyme in the food material to be treated can be rapidly inhibited, which can quickly and effectively inhibit the generation of hypoxanthine, thereby reducing the generation of hypoxanthine, reducing the risk of hyperuricemia and gout, and having little effect on the taste and quality of the food material.

The invention relates to methods of identifying enzymes and methods of screening for optimised enzymes for degrading polymers such as plastics. The invention also relates to polymer degrading enzymes and nucleic acids encoding these enzymes as well as expression cassettes and host cells comprising the nucleic acids. The invention also relates to uses of these enzymes or the host cells comprising the nucleic acids encoding these enzymes to degrade polymers such as plastics. Lastly, the invention also relates to the crystal structure of plastic degrading enzymes and uses of these structures.

The present application relates to a novel PET-degrading enzyme and a use thereof.

The invention belongs to the technical field of genetic engineering, and discloses application of an anchoring protein INP-N in a PET degrading enzyme system displayed on the surface. According to the invention, after efficient anchoring protein INP-N is screened, FAST-PETase is mediated by the efficient anchoring protein INP-N to construct recombinant escherichia coli of which the surface displays PET degrading enzyme. Furthermore, hydrophobin HFBII is introduced into the surface display system, so that the relative enzyme activity of the PET degrading enzyme is improved by 1.1 times or more. Meanwhile, FAST-PETase and carboxylesterase Est30KL are synchronously expressed, so that the proportion of TPA in a degradation product can reach 95% or above. In addition, the PET degrading enzyme surface display system provided by the invention is excellent in cycle performance, more than 65% of activity can still be retained when PET is cyclically degraded for the third time, and the application cost can be greatly reduced. The method can be applied to the fields of degradation of PET products, preparation of PET degradation agents or BHET degradation agents and the like.

The invention relates to the field of enzyme engineering, in particular to a mutant of nylon degrading enzyme and application of the mutant. The mutant of the nylon degrading enzyme is obtained by mutation at one or more key sites through stability modification guided by machine learning, substrate channel inlet loop region modification and tetramer interface modification on the basis of a wild type nylon degrading enzyme NylCp2-TS as shown in SEQ ID NO: 1; the mutant enzyme can efficiently degrade nylon materials in various forms, including films, particles and industrial fibers of nylon 6 and nylon 66, and has a wide application prospect in the field of nylon waste biodegradation.

This invention discloses a method for promoting the biodegradation of N,N-dimethylformamide and simultaneous denitrification. The method constructs a zero-valent iron (ZFE)-coupled biological treatment system, which not only accelerates electron transfer efficiency but also significantly increases the activity of DMF-degrading enzymes and nitrate reductases, synergistically promoting electron transfer and microbial metabolic activity, thereby enhancing the removal of DMF and nitrate nitrogen. Compared to biological treatment systems that only add activated sludge without adding ZFE, adding ZFE significantly improves the removal efficiency of DMF and nitrate nitrogen, making it suitable for the treatment of large-scale polluted water bodies or industrial wastewater.

The application provides a hot liquid source quorum quenching strain Shiquan Haeyuan bacteria and application thereof, the strain screened can inhibit pathogenic bacteria and is used for preparing bacteriostatic drugs; the preservation number of the Shiquan Haeyuan bacteria screened is CCTCC NO: M 20251224.The application also provides an AHL degradation enzyme Aii937 protein separated from the YESM3 strain, characterized in that the amino acid sequence of the protein is SEQ ID NO: 2.The YESM3 strain of the application can block the QS path of pathogenic bacteria through AHL lactonase Aii937, reduce the secretion of virulence factors of pathogenic bacteria; the strain YESM3 has no pathogenicity.AHL lactonase does not have broad spectrum, has strong substrate specificity, and only degrades C6-HSL and 3OC-C 14 -HSL.

The invention provides a method for improving the molecular weight of polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short) synthesized by microorganisms, and relates to a method for improving the molecular weight of the polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short). By knocking out or knocking down endogenous (intracellular) carboxylesterase, lipase, esterase, thioesterase and PHA degrading enzyme genes of bacteria for synthesizing PHA, the molecular weight of various synthesized PHA is obviously improved. The method has universality for improving the molecular weight of various PHAs synthesized by different species of bacteria, the mechanical property of the PHA can be improved by improving the molecular weight of the PHA, and new performance is provided for expanding the application of the PHA.

The application discloses a method for recovering terephthalic acid from polyester-containing products, which comprises the following steps: (a) performing an enzymatic reaction by contacting the polyester-containing products with a degradation enzyme, using ammonia water / ammonia gas / liquid ammonia / ammonium hydroxide as a pH regulator in the enzymatic reaction, and forming a degradation solution containing ammonium terephthalate and a dihydric alcohol after the enzymatic reaction; (c) performing a heating treatment on the degradation solution; and (d) obtaining solid terephthalic acid through solid-liquid separation. Through the cycle mode of water dissolution-reconcentration-again deamination, the total recovery rate of PTA is significantly improved to more than 90%, efficient utilization of materials is realized, and the whole process still does not need to add acid and alkali, no waste salt is generated, and the process is green and economical.

The invention belongs to the technical field of composite strains, particularly relates to a high-lignin straw fermentation composite microbial agent, and a preparation method and a fermentation process thereof, and aims to solve the problems of low lignin degradation efficiency, long period and high cost of a single microorganism in the prior art. According to the invention, a composite synergistic system of coriolus versicolor, bacillus subtilis and pichia pastoris is innovatively adopted, and efficient degradation is realized through a mechanism that the coriolus versicolor secretes a lignin degrading enzyme system, the bacillus subtilis assists cellulose decomposition and the pichia pastoris optimizes a microenvironment; the preparation method of the complex microbial inoculant comprises the following steps: culturing various strains in a proper culture medium to a specific growth state, and mixing according to an optimized volume ratio; the fermentation process relates to high-lignin straw pretreatment, inoculation, sealed fermentation under control conditions and regular ventilation, the lignin degradation rate can be increased, the feed nutritional value can be improved, the relative feeding value and quality can be improved, and economic and environment-friendly technical support is provided for resource utilization of agricultural by-products.

The invention discloses a sea cucumber feed as well as a preparation method and application thereof, and belongs to the technical field of sea cucumber feed preparation. According to the technical scheme, every 100 g of feed comprises 40 g of sea mud, 15-25 g of gulfweed powder, 5-15 g of enzymolysis gulfweed liquid and 1 g of multi-vitamin multi-mineral premix. The dosage of the enzymolysis gulfweed liquid is calculated according to the dry weight of the gulfweed, and the enzymolysis gulfweed liquid is obtained by performing enzymolysis on the gulfweed through algin degrading enzyme, pectinase and cellulase. The sea cucumber feed is applied to the aspect of sea cucumber feeding, solves the problem that the existing sea cucumber feed still cannot well promote accumulation of sea cucumber saponin and fucose in the body wall of the sea cucumber so as not to improve the nutritional value of the sea cucumber, and can effectively promote accumulation of sea cucumber saponin and fucose in the body wall of the sea cucumber so as to improve the nutritional value of the sea cucumber.

This invention discloses a polyethylene terephthalate (PET) hydrolase, PmPETase, and its applications. The hydrolase PmPETase is a protein with the amino acid sequence shown in SEQ ID NO:1. This invention constructs an expression system suitable for PmPETase, obtains a recombinant enzyme protein with catalytic activity, and comprehensively analyzes its substrate specificity, catalytic properties, and potential plastic degradation ability. This provides a new theoretical basis and application reference for the development and utilization of PET degrading enzymes and green treatment technology for polymer materials.

The invention belongs to the technical field of microorganisms, and particularly relates to a microvesicle bacterium capable of degrading sargassum horneri and application of the microvesicle bacterium. The microvesicles are microvesicles okinawensis, the microvesicles are preserved in the China General Microbiological Culture Collection Center on October 27, 2025, and the preservation number of the microvesicles is CGMCC (China General Microbiological Culture Collection Center) No. 36355. The microvesicle bacterium of the sargassum horneri component degrading enzyme is screened from rotten sargassum horneri, cell walls of the sargassum horneri can be efficiently cracked, release of effective components in brown algae is promoted, and therefore utilization of sargassum horneri resources is promoted.

The application discloses a degradation enzyme PufH for efficiently decomposing polyurethane sponge and application thereof. The amino acid sequence of the degradation enzyme is shown as SEQ ID NO. 1, and the coded gene sequence is shown as SEQ ID NO. 2. The degradation enzyme PufH used in the application can reach a degradation rate of 82.4% on commercial polyurethane sponge within 48 hours. The degradation effect of the degradation enzyme PufH on commercial polyurethane sponge is superior to that of the currently reported PU degradation enzyme, and the degradation enzyme PufH can be applied to the recycling or pollution repair of polyurethane sponge waste.

The application discloses a multifunctional fiber-degrading enzyme and application thereof, and belongs to the technical field of biology. The multifunctional fiber-degrading enzyme is named RuCelF, and the amino acid sequence is shown as SEQ ID NO. 1. Based on rumen microbial metagenomic resource mining technology, a new multifunctional fiber-degrading enzyme RuCelF is developed. The enzyme has double activities of cellulase and xylanase, can be used for degrading agricultural waste, reduces treatment cost, improves degradation efficiency, helps to improve the utilization rate and economic benefits of agricultural waste, and provides a new enzyme source and technical support for efficient resource utilization of agricultural waste.

The invention relates to the field of bio-enzyme catalysis, and discloses a bio-enzyme catalysis process for reducing agent modification, which comprises the following steps: S1, enzyme solution preparation: mixing a bio-enzyme preparation with a buffer solution with the pH value of 5-8 to prepare an enzyme solution with the enzyme concentration of 0.5-5.0 g / L; the biological enzyme preparation is a compound enzyme composed of one or more of cellulase, hemicellulase and lignin degrading enzyme; s2, enzymolysis modification: mixing a carbonaceous reducing agent raw material with the enzyme solution prepared in the step S1 according to a mass ratio of 1: 5-1: 20, carrying out an enzymolysis reaction at 40-60 DEG C, and meanwhile, applying ultrasonic waves to carry out auxiliary treatment; the power of the ultrasonic waves is 100-500 W, and the frequency of the ultrasonic waves is 20-40 kHz And S3, solid-liquid separation: after the reaction is finished, separating the modified reducing agent from the enzymatic hydrolysate to obtain the solid modified reducing agent. Compared with the prior art, the method has the advantages that a biological enzyme catalysis process for modifying the reducing agent is provided, the energy consumption is low, the environment is protected, the cost is low, and the performance of the reducing agent can be remarkably improved.

The invention discloses a polyglutamic acid degrading enzyme and application thereof in controllable preparation of gamma-PGA with different molecular weights. The invention provides a degrading enzyme derived from fermented bacillus glycinium.Polyglutamic acid degrading enzyme is obtained by fusing a solubilizing NT11 tag and a degrading enzyme with a signal peptide PGAase sequence removed, and the amino acid sequence of the polyglutamic acid degrading enzyme is shown as SEQ ID NO.7. The polyglutamic acid degrading enzyme achieves soluble expression (the proportion is 60% or above) under low-temperature and low-concentration induction, and the enzyme activity reaches 300 U / mL. In the enzymatic hydrolysis reaction, the gamma-PGA with the molecular weight of 50-400 kDa can be prepared by regulating the time (0-7 h) and the enzyme adding amount (0.1-1 U / g), the batch stability is high, the application range of the PGA is expanded, and the market demand is improved.

The invention discloses a nutrient degrading enzyme, an engineering bacterial agent and application of the engineering bacterial agent in regulation and control of polymerized slow-release fertilizer nutrients, and belongs to the technical field of enzyme engineering. According to the present invention, with the artificial intelligence modified specific polymerization slow release fertilizer degrading enzyme, the degradation activity of the source strain enzyme can be retained, the efficient heterologous expression can be achieved, and the dual improvement of the stability and the catalytic efficiency can be promoted; the constructed composite engineering strain has no antagonistic effect, and low-cost large-scale production of the degrading enzyme can be realized. The compound engineering strain can realize accurate regulation and control of nutrient release of the polymerized slow-release fertilizer, realizes accurate supply of nutrient requirements in different stages of the whole growth period of different crops by adjusting the proportion of engineering strains for producing peptidase and amidase, realizes complete degradation of the polymerized slow-release fertilizer, greatly improves the crop yield and the nutrient utilization rate, and has wide application prospects. The method shows an important application prospect in the aspect of precision agriculture development.

The invention provides a method for preparing edible alcohol by fermenting wheat starch, which comprises the following steps: pretreatment, enzymolysis, composite flora fermentation and purification. The pretreatment method comprises the following steps: sieving wheat B starch with a 90-110-mesh sieve, adding water to prepare 32wt%-35wt% starch milk, adding a composite pre-oxidant, carrying out heat preservation for 40-50 minutes under the conditions that the temperature is 46-50 DEG C and the pH value is 4.9-5.2, then adding a composite degrading enzyme, and carrying out heat preservation for 20-25 minutes, so as to obtain pretreated starch milk; the method finally realizes double improvement of product quality and production benefit, the viscosity of the pretreated starch milk is 270-300 cP, the reducing sugar of the converted mash is 213-232 g / L, the fermentation time is 48-52 h, the residual total sugar of the fermented mature mash is 1.5-1.7 g / L, the purity of the prepared alcohol reaches 96.3%-96.6%, the yield is 0.459-0.466 L / kg starch, the content of n-propyl alcohol is 0.2-0.4 mg / L, and the content of isobutanol and isoamyl alcohol is 0.3-0.6 mg / L.

The invention provides oral cyclodextrin inclusion complex formulations in which inclusion complexes of borneol and trans-cinnamaldehyde are provided together with nattokinase and with an enzyme having a cyclodextrin-degrading activity capable of digesting cyclodextrins, so that upon delivery of the formulation to a target tissue the CD- degrading enzyme is activated and releases the borneol and trans-cinnamaldehyde from the cyclodextrins. These cyclodextrin inclusion complex formulations may be used for treating atheromatous plaque and related diseases.