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Trichodermaviride used for producing cellulose degrading enzyme and its application in urban landscaping waste degradation

A technology of Trichoderma viride and cellulase, applied in the field of microorganisms, can solve the problems of insufficient research on Trichoderma viride and few applied researches.

Inactive Publication Date: 2012-07-11
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Trichoderma viride is a woody enzyme that has been widely concerned in recent years and can be used to control waste crop pollution and high-yield cellulase strains. This genus of bacteria can degrade a variety of agricultural wastes, including corn cobs, corn stalks, straw, etc., but Domestic research on Trichoderma viride is not deep enough, and there are few researches on its application

Method used

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  • Trichodermaviride used for producing cellulose degrading enzyme and its application in urban landscaping waste degradation
  • Trichodermaviride used for producing cellulose degrading enzyme and its application in urban landscaping waste degradation
  • Trichodermaviride used for producing cellulose degrading enzyme and its application in urban landscaping waste degradation

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Embodiment 1

[0028] Trichoderma viride M20 belongs to the phylum Deuteromycota, Hyphospora, and was isolated in 2009 from the soil below the pile of straw soil in Shanxi. This strain grows well on PDA solid medium. The culture temperature is 30 ℃, and it can be matured after 3-5 days of cultivation. Green spores. Preparation method of PDA plate: Peel 200g potato, cut into pieces, boil for 30 minutes, then filter with gauze to get the filtered juice, then add 1% glucose, 2% agar, and then set the volume to 1 liter, the pH is natural.

[0029] Genomic DNA of Trichoderma viride M20 was extracted, PCR was amplified with ITS universal primers (forward primer ITS1: 5'-TCCGTAGGTGAACCTGCGG-3', reverse primer ITS4: 5'-CCTCCGCTTATTGATATGC-3'), and the sequence was Blastn homologous compare with Trichoderma viride Has a high similarity (99%), so named Trichoderma viride. Trichoderma viride , No. M20, preserved in China General Microorganism Culture Collection Center on April 2, 2011, and the preser...

Embodiment 2

[0032] Trichoderma viride M20 grows in the medium of 2% fallen leaves and lawn grass without adding any inorganic salt, and promotes the decomposition of fallen leaves and lawn grass, so it can be effectively used in the treatment of urban greening waste.

Embodiment 3

[0034] Trichoderma viride M20 carries out submerged fermentation in the inorganic salt enzyme medium, and the medium composition is: 0.05% (NH4 ) 2 SO 4 , 0.05% L-Aspartic Acid, 0.1% KH 2 PO 4 , 0.05% KCl, 0.041% MgSO 4 7H 2 0, 0.01% CaCl 2 , 0.05% yeast powder, 0.1%~4% cellulose, of which cellulose includes microcrystalline cellulose, straw powder, corncob powder, straw powder, bran, etc. M20 had the highest cellulase activity induced by microcrystalline cellulose. Growth with 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4% microcrystalline cellulose as the only carbon source can produce cellulase, except for 0.5% and 1% microcrystalline The cellulase filter paper enzyme activity measured in the cellulose group was relatively large, while the other experimental groups were relatively small. Lower Avicel requirements can reduce the cost of cellulase production. Cellulase assay method: Accurately weigh 50mg of Whatman filter paper (Cat No. 1001 110) (specification...

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Abstract

The invention belongs to microbe technical field, and especially relates to a Trichodermaviride, and the invention also relates to an application of Trichodermaviride. The Trichodermaviride strain capable of producing cellulase with high-yield is Trichodermaviride in Trichoderma, the code number is M20, and the Trichodermaviride is preserved at China General Microbiological Culture Collection Center on 2nd, April, 2011, the preservation number is CGMCC No.4732. The strain of the invention can be grown in a medium which takes a plurality of cellulose compounds as a sole carbon source, the cellulose compounds comprise carboxymethylcellulose sodium, microcrystalline cellulose, corn straw, cob, deciduous leaf, turf grass and the like. The Trichodermaviride of the invention can secrete a plurality of cellulose hydrolases, can be used for processing urban landscaping waste, can acquire high reducing sugar in short time, the obtained reducing sugar can be used for producing single cell protein and can be used for producing fuel ethanol, and the Trichodermaviride possesses important meaning for solving a series of problems of energy shortage and environmental pollution at present.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and particularly relates to a kind of Trichoderma viride; in addition, the invention also relates to the application of the Trichoderma viride. Background technique [0002] Cellulose is the largest renewable resource in nature. Taking straw as an example, the world produces about 100-200 billion tons of crop straw every year, but only a few hundred million tons of cellulose are used by humans. my country is a large agricultural country. According to rough statistics, our country produces about 700 million tons of crop straw every year. Because there are many high-energy hydrogen bonds in cellulose, it is difficult to hydrolyze and utilize it, and most of the cellulose substances are discarded. Only a small part of the large amount of cellulose is used in textiles, feed and papermaking; the vast majority is used as fuel or returned to the carbon cycle in nature through natural decompositi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/14B09B3/00C12R1/885
Inventor 赵心清孙鸿金白凤武
Owner DALIAN UNIV OF TECH
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