Biomass pretreatment method
A biomass and pretreatment technology, applied in the direction of biofuels, fermentation, etc., can solve the problem of incomplete understanding of the connection, the failure to find the connection between lignin, cellulose and hemifiber structure, and achieve the effect of promoting hydrolysis, low cost, and increased The effect of nitrogen nutrition
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[0021] Cut and pulverize the wheat straw into powder, put it into a heatable and heat-preserving container, boil and sterilize, lower it to room temperature and adjust the acid to pH4.8, then add acid protease 50mg / g straw, stir well, and enzymatically hydrolyze at 50°C 48 hours. Then add cellulase, the enzyme activity is 3000U / g, the added amount is 50mg / g straw, enzymolyze at 50℃ and pH4.8 for 48 hours, and remove the monosaccharide produced after hydrolysis by extrusion, rinsing and other methods. For subsequent ethanol fermentation, the filter residue is a mixture of lignin and hemicellulose, which can be further processed.
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