Biomass pretreatment method
A biomass and pretreatment technology, applied in the direction of biofuels, fermentation, etc., can solve the problem of incomplete understanding of the connection, the failure to find the connection between lignin, cellulose and hemifiber structure, and achieve the effect of promoting hydrolysis, low cost, and increased The effect of nitrogen nutrition
Inactive Publication Date: 2010-07-14
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology
[0004] The reason for the lack of current biomass pretreatment technology lies in the incomplete understanding of the types of biomass components, the structure of each component, especially the structure of lignin, and the relationship between each component, and the lack of lignin, cellulose and semi-organisms. The main reason for the structural connection between the three fibers, so it is too dependent on the improvement of the chemical and physical pretreatment methods themselves
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Embodiment
[0021] Cut and pulverize the wheat straw into powder, put it into a heatable and heat-preserving container, boil and sterilize, lower it to room temperature and adjust the acid to pH4.8, then add acid protease 50mg / g straw, stir well, and enzymatically hydrolyze at 50°C 48 hours. Then add cellulase, the enzyme activity is 3000U / g, the added amount is 50mg / g straw, enzymolyze at 50℃ and pH4.8 for 48 hours, and remove the monosaccharide produced after hydrolysis by extrusion, rinsing and other methods. For subsequent ethanol fermentation, the filter residue is a mixture of lignin and hemicellulose, which can be further processed.
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The invention relates to a biomass pretreatment method which can be a substitute for alkali, acid and steam explosion and reduce the cost and environmental pollution. The invention adopts the technical scheme that: crushing the biomass into powder, sterilizing, cooling, then adding proteinase, hydrolyzing protein in the biomass into free amino acids, and then using other methods to hydrolyze cellulose and hemicellulose into monose for alcoholic fermentation. The proteinase comprises acid protease, alkali protease or collagenase and the like. The biomass comprises crop straws, grass, wood or fibrous plants.
Description
1. Technical field: [0001] The invention relates to a biomass energy method, in particular to a biomass pretreatment method, which is a method for pretreating crop stalks with protease. 2. Background technology: [0002] Current microorganisms that can directly break down lignin and hemicellulose are slow to act. The direct use of cellulase cannot effectively hydrolyze straw directly. The pretreatment of removing lignin and hemicellulose in straw by heating, adding inorganic acid, alkali, etc. is the key to the production of ethanol by biomass fermentation. Alkaline pretreatment can remove lignin, but the wastewater is seriously polluted. Acid method can remove hemicellulose, but it is easy to produce furan and small molecule acids that inhibit the growth of yeast. The steam explosion method has high cost, complex products, and tar formation. . [0003] Finding a pretreatment method that is cheap, does not increase sodium, sulfur and other chromogenic elements, and does n...
Claims
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IPC IPC(8): C12P21/06C12P19/14C12P7/10
CPCY02E50/16Y02E50/10
Inventor 王联结马英辉效必亮
Owner SHAANXI UNIV OF SCI & TECH
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