78 results about "Trichoderma viride" patented technology

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention relates to a trichoderma virens strain and application thereof. According to the invention, a Trichoderma virens strain GZUAS831026 is separated from a capsicum land soil sample, the Trichoderma virens strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the Trichoderma virens strain is GDMCC No: 65370. The trichoderma virens strain obtained by the invention has a good pepper straw degradation effect and a growth promoting effect, has a good inhibition effect on pathogenic bacteria of the ginger tuber rot disease, and can be used for preventing and treating the ginger tuber rot disease.

The invention discloses a trichoderma virens preparation capable of preventing diseases and increasing yield as well as a preparation method and application of the trichoderma virens preparation. Belongs to the technical field of biological agents. According to the trichoderma virens strain T259 disclosed by the invention, the trichoderma virens strain T259 is respectively subjected to strain amplification culture, liquid amplification culture and solid fermentation culture, a solid fermentation product of the T259 strain is successfully prepared, the solid fermentation product is mixed with an auxiliary agent, and the trichoderma virens preparation capable of preventing diseases and increasing yield is prepared. The trichoderma virens spore content in the preparation is improved; solid fermentation can effectively improve the colonization ability of trichoderma virens in the field. The production of the trichoderma virens preparation has the characteristics of large sporulation quantity, short period, wide raw material source and low cost; the fertilizer is easy to use and operate, suitable for various crops and remarkable in disease prevention and yield increase effect. And a foundation is laid for quality and efficiency improvement and high-quality and high-efficiency development of modern agriculture.

The invention relates to the technical field of soil improvement, in particular to a soil conditioner for improving desert saline-alkali soil and application of the soil conditioner. The soil conditioner is prepared from 5.5 to 7.5 * 10 < 4 > kg of yellow water, 5 to 6 * 10 < 3 > kg of crop straw, 60 to 76 kg of phanerochaete chrysosporium, 50 to 60 kg of bacillus amyloliquefaciens J10, 30 to 46 kg of trichoderma viride, 30 to 50 kg of lactobacillus kefir CP423, 1 to 1.2 * 10 < 4 > kg of organic fertilizer, 0.8 to 1.2 * 10 < 3 > kg of amino acid water-soluble fertilizer, 25 to 35 kg of nitrogen fertilizer and 8 to 12 kg of phosphate fertilizer. After the soil conditioner is applied to ecological improvement, the content of organic matter, total nitrogen, total phosphorus, alkali-hydrolyzable nitrogen and available phosphorus can be effectively increased, the soil quality is remarkably improved, and the yield and quality of planted crops can be remarkably improved.

The application provides a solid substrate for soilless culture prepared by fermentation of coffee residue and mixed bacteria, wherein the mixed bacteria include yeast, bacillus subtilis and trichoderma viride; the weight ratio of the yeast, the bacillus subtilis and the trichoderma viride is 2:1:3. The solid substrate for soilless culture is rich in trace elements required by plant growth, has low bulk density, high water holding porosity, and is controllable. The solid substrate for soilless culture can effectively improve the plant growth environment, has disease resistance and insect resistance, and improves the survival rate of plants.

The present invention relates to the technical field of biological control, and particularly to a compound microbial agent for preventing and controlling plant diseases and its application. The microbial combination provided by the present invention includes Bacillus subtilis BLG010, Bacillus amyloliquefaciens HW05, Paecilomyces lilacinus E16, Trichoderma viride H06, Bacillus velezensis LG14-3 and Bacillus amyloliquefaciens HC200 which have a synergistic effect; a compound microbial agent is also provided, and the agent includes the microbial combination, a carrier and a synergist provided by the present invention, and also includes a healthy plant leaf extract or a healthy plant rhizosphere soil extract. The agent has a control effect on a variety of major plant diseases (pepper blight, banana wilt, tomato wilt and bacterial wilt, citrus huanglongbing, tea blister blight and tea target spot), and at the same time can change the fungal or bacterial diversity of plant leaves or rhizosphere soil, as well as increase the abundance of beneficial microorganisms or reduce pathogenic microorganisms.

A biocontrol formulation for plant pathogens includes a Trichoderma viride filtrate and an inert substance selected from the group consisting of a viscosity reducer, a surfactant, flour, clay, and combinations thereof. In an embodiment, the filtrate includes conidia of the Trichoderma viride. In an embodiment, the filtrate includes byproducts obtained from the fermentation of Trichoderma viride. In an embodiment, the byproducts include at least one of the organic acids, growth regulators, and amino acids.

The invention relates to the technical field of solid waste resourceful treatment, and discloses coal gangue-based improved soil and a preparation method thereof. The coal gangue-based improved soil comprises the following raw materials: coal gangue, sandy soil and a fermentation inoculant, the fermentation bacterial agent is prepared from bacillus subtilis SHBCC D11446, arbuscular mycorrhizal fungi SHBCC D24696, trichoderma viride SHBCC D10197 and bacillus velezensis GS02, and the preservation number of the bacillus velezensis GS02 is CCTCC (China Center for Type Culture Collection) NO: M 2025531. Phosphorus, potassium, nitrogen and organic matters in the coal gangue can be fully utilized, the prepared coal gangue-based improved soil has high nutrient substance content and low effective state heavy metal content, and meanwhile, the situation that the alkalinity and the salt content of the improved soil are greatly improved due to introduction of the coal gangue can be avoided; the improved soil can effectively promote plant growth.

The invention provides an ensilage fermented feed and a preparation method thereof, which adopt a staged sequential fermentation strategy: firstly, trichoderma viride secretes cellulase under an aerobic condition to decompose fibers and release soluble sugar, and then lactobacillus rhamnosus quickly produces acid under an anaerobic environment to inhibit putrefying bacteria; the digestibility and the stability of the feed are obviously improved; meanwhile, pH-responsive nutritional microcapsules are also added into the raw materials during preparation of the ensilage fermented feed: pectin oligosaccharide, chitosan and cassava starch are used as wall materials, rosmarinic acid, calcium lactate and bacillus coagulans are embedded, and core materials are released by stages through pH responsiveness, so that the ensilage fermented feed is prepared; the nutritional value of the feed is improved, digestion and absorption are promoted, and the intestinal health of animals is improved.

This invention provides a *Trichoderma viride* RM9 strain antagonizing jasmine white mold disease and its application. The *Trichoderma viride* RM9 strain of this invention is protected under the accession number CCTCC M 20221442. *Trichoderma viride* RM9 of this invention is isolated from the rhizosphere of jasmine plants, exhibits strong rhizosphere colonization ability, and demonstrates good control efficacy against jasmine white mold disease, achieving a control effect of 83.34%. It also promotes the growth of jasmine seedlings. Furthermore, its cultivation conditions are relatively simple, and its development into a solid inoculant is beneficial for improving jasmine yield and economic benefits.

The invention relates to the field of multi-enzyme organic fertilizers, and discloses a biological bacterium fermentation multi-enzyme organic fertilizer and a preparation method of the biological bacterium fermentation multi-enzyme organic fertilizer. 12 to 20 parts of soybean meal; 5-10 parts of a humic acid raw material; 3-8 parts of a mineral additive; 2-5 parts of sugar auxiliary materials; 0.5 to 1.5 parts of a functional microbial agent; 1.0 to 2.5 parts of trichoderma viride powder; 0.8 to 1.5 parts of sodium alginate; and 1.5 to 3.0 parts of xylooligosaccharide. The trichoderma viride powder is utilized to secrete cellulase, xylanase and protease, organic waste such as straw and soybean meal is effectively degraded and converted into nutrients capable of being absorbed by crops, meanwhile, plant hormones secreted by the trichoderma viride can stimulate root development, promote branch extension, enhance nutrient absorption and improve the growth vigor of the crops, and the yield of the crops is increased. The effectiveness of phosphorus in the soil and the fertilizer utilization efficiency are obviously improved.

The invention relates to a method for separating and purifying active substances in trichoderma citrinoviride fermentation liquor, in particular to a method for separating and purifying trichoderma citrinoviride fermentation liquor. Comprising the steps of extraction of trichoderma citrinoviride fermentation liquor and purification and analysis of active substances in the trichoderma citrinoviride fermentation liquor, the trichoderma citrinoviride fermentation liquor is subjected to ethyl acetate extraction and vacuum concentration to obtain a crude extract, an efficient preparative liquid phase is adopted for separation and purification in the purification and analysis step, and methanol and formic acid water are selected in the purification and analysis step. Through the steps of extracting the trichoderma citrinoviride fermentation liquor and purifying and analyzing the active substances in the trichoderma citrinoviride fermentation liquor, the separation and purification process of the trichoderma citrinoviride fermentation liquor is simple, the cost is low, the operation is simple and convenient, and the product purity is high.

The invention discloses a bio-based fertilizer and application thereof. The preparation method comprises the following steps: S1, crushing the mango pomace and the bagasse, sieving the crushed mango pomace and bagasse with a 40-60-mesh sieve, adding diluted hydrochloric acid and ferrous sulfate, mixing the crushed mango pomace and bagasse, carrying out a hydrolysis reaction, and filtering a hydrolysis product to obtain solid residues; s2, seed liquid of bacillus coagulans, bacillus pseudomycoides, trichoderma viride and saccharomyces cerevisiae is inoculated into the mixture of the solid residues and the cattle and sheep manure for fermentation, and a fermentation product is obtained; s3, mixing the fermentation product, charcoal and polyglutamic acid to prepare the fertilizer. The mango pomace, solid bagasse and cattle and sheep manure mixture are cooperatively fermented through compound bacteria, the porosity of the fertilizer is increased, the water and fertilizer retention capacity is improved, the fermentation product, biochar and polyglutamic acid are mixed and granulated to prepare the fertilizer, the disintegration rate can be regulated and controlled, nutrients are prevented from being instantly released, and root growth and microorganism colonization are promoted.

The invention provides a trichoderma preparation for improving the yield and quality of sugarcane as well as a preparation method and application of the trichoderma preparation, and belongs to the technical field of microbial preparations. The trichoderma preparation is prepared from the following components in parts by mass: 15-25 parts of trichoderma viride, 10-20 parts of trichoderma koningii and 2-4 parts of a seaweed extract. The trichoderma viride, the trichoderma koningii and the seaweed extract can achieve a synergistic effect, and absorption and utilization of the sugarcane on nutrient substances are promoted. The trichoderma preparation is sprayed once in a sugarcane seedling stage and a stem node elongation stage respectively, so that the yield and the quality of the sugarcane can be remarkably improved.

The invention belongs to the technical field of fertilizers. The invention provides a waste fruit fermentation compound fertilizer and a preparation method thereof.The preparation method comprises the steps that S1, 54% by mass of walnut green seedcase, 33-35% by mass of edible fungus residues and 11-13% by mass of plant ash are mixed to be uniform, and a basic material is obtained; s2, adding a functional component accounting for 8.5-10% of the mass of the basic material into the basic material, and uniformly mixing to obtain a fermentation base material; the functional components comprise dolomite powder and rice hull ash; s3, adding 0.1-0.12% by mass of a compound fermentation inoculant into the fermentation base material, uniformly mixing, and then composting and fermenting to obtain the waste fruit fermentation compound fertilizer, wherein the mass of the compound fermentation inoculant is 0.1-0.12% of that of the fermentation base material; the compound fermentation inoculant is prepared by uniformly mixing mixed bacteria and wheat bran according to a mass ratio of 1: (4-4.5); the mixed bacteria comprise trichoderma viride, bacillus subtilis, aspergillus oryzae, aspergillus niger, saccharomycetes and lactic acid bacteria. The fertilizer efficiency of the compound fertilizer prepared from the waste fruit / abandoned fruit walnut green seedcase as the raw material is effectively improved, and the yield of the pakchoi is further improved.

The invention provides a trichoderma inoculant, a preparation method thereof and application of the trichoderma inoculant in improving yield and quality of beet, and belongs to the technical field of agricultural microbial inoculants. The trichoderma agent comprises the following components: trichoderma harzianum, trichoderma viride, purpureocillium lilacinum, bacillus amyloliquefaciens and bacillus laterosporus in a mass ratio of (3-5): (1-3): (2-4): (2-4): (1-2). According to the invention, the strains cooperate with each other to promote the growth of beet, effectively enhance the immune resistance of beet, and effectively prevent and reduce beet root rot, brown blotch and other diseases caused by continuous cropping; the photosynthesis efficiency of the beet can be enhanced, the accumulation of photosynthetic products is improved, the soil structure is improved, the number of beneficial bacteria groups in the soil is increased, and the rhizosphere environment is improved, so that the sugar content and yield of the beet are increased. By applying the microbial agent disclosed by the invention, multiple effects of increasing the yield per mu, increasing the sugar content of beet, improving soil, improving the fertilizer efficiency, resisting diseases, preventing insects and the like can be achieved, and farmers are helped to increase both production and income.

The invention relates to the technical field of biological environmental protection, and discloses a compound microbial agent for constructing micro-ecological balance based on five florae. Comprising the following raw materials in parts by weight: 3-5 parts of photosynthetic bacteria, 1-2 parts of lactic acid bacteria, 2-4 parts of yeast acid, 2-5 parts of bacillus, 2-5 parts of actinomycetes, 1-2 parts of acetic bacteria, 2-3 parts of trichoderma viride, 2-4 parts of bifidobacterium, 2-3 parts of lactobacillus plantarum, 1-2 parts of nitrifying spina bacteria, 10-14 parts of corn, 10-14 parts of soybeans, 10-14 parts of sorghum and 1-3 parts of aspergillus. According to the compound microbial agent with micro-ecological balance constructed based on the five flora, the aspergillus is decomposed into monosaccharide by utilizing energy, the monosaccharide becomes food of saccharomycetes, the monosaccharide is converted into alcohol after being subjected to saccharomycetes bacterial decomposition, the alcohol is converted into acetic acid by acetic acid bacteria, and carbon dioxide generated by the saccharomycetes is utilized by photosynthetic bacteria to prepare glucose; the microbiota complement each other to live together, and micro-ecological balance is constructed through the five microbiota.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The application provides a degradable soilless and trayless seedling raising blanket, which is in a plate structure and comprises a biological base material and a biological binder; the biological binder is obtained by fermentation of a lees enzymolysis solution and a synergist after inoculation of a strain; the lees enzymolysis solution is prepared from fresh lees and glucoamylase; the synergist is prepared from natural herbs, white sugar, deionized water and a bacterial solution according to a mass ratio of 1:15:150:15; the bacterial solution comprises Trichoderma viride JX18-0201; the Trichoderma viride JX18-0201 has a preservation number of CCTCC NO:M2021509. The technical principle of the application is that the blocky seedling raising blanket formed by the biological base material and the biological binder replaces the original traditional seedling raising substrate and plastic seedling tray, can meet the nutrition of normal seedling emergence and development of plants, has the characteristics of the plastic seedling tray, can be directly used for seedling transplanting, and does not need to use the plastic seedling tray, thereby reducing the cost of recycling and processing.

The invention provides a crop nutritional supplement containing glycopeptide as well as a preparation method and application of the crop nutritional supplement, and belongs to the technical field of biological agriculture. The crop nutritional supplement comprises fish protein peptide, fucose oligosaccharide, fulvic acid, silicon-calcium-potassium-magnesium fertilizer, bone meal, polyglutamic acid, a microbial agent and a carrier. Wherein the fish protein peptide is obtained by fermenting deep-sea fish meat with bacillus subtilis SH21 and performing enzymolysis with compound enzyme, and the microbial agent is prepared from trichoderma atroviride C61 and paenibacillus mucilaginosus HB-02. The crop nutritional supplement can activate the immune system of crops, enhance disease resistance, promote nutrient absorption and improve the yield and quality of crops, is environment-friendly, and can solve the problems caused by chemical pesticides and traditional chemical fertilizers.

The invention discloses Trichoderma virens (Trichoderma virens) and an application of the Trichoderma virens (Trichoderma virens). The invention provides a Trichoderma virens strain with strong hyperparasitism on peanut sclerotium rolfsii and sclerotinia sclerotiorum, the Trichoderma virens strain is named as MJ18, is preserved in China Center for Type Culture Collection (CCTCC) on January 15, 2025, and has a preservation number of CCTCC NO: M 2025149. The trichoderma virens MJ18 can be used for inhibiting peanut sclerotium rolfsii and sclerotinia sclerotiorum, can also be used for inhibiting plant pathogenic fungi such as pythium ultimum, fusarium graminearum, fusarium pseudograminearum and rhizoctonia solani, and can be used for preventing and treating related plant diseases caused by the pathogenic fungi. Besides, the trichoderma virens MJ18 can improve the emergence rate of the peanuts and promote the growth of the peanuts in the seedling stage, can be combined with chemical insecticides for use, and can be used as a seed coating agent to promote the germination of the peanuts and realize the common prevention and control of the southern blight of the peanuts and the insect pests of the peanuts at the same time.

The invention provides an organic fertilizer suitable for tea garden soil, the organic fertilizer comprises the following components by weight: 80 parts of tea residue, 75 parts of manure, 175 parts of tea seed cake, 65 parts of illite powder, 30 parts of agricultural rare earth, 25 parts of ammonium hydrogen phosphate, 3 parts of trichoderma viride, 65 parts of calcium magnesium phosphate fertilizer, 40 parts of nitrogen fertilizer, and 15 parts of plant ash, the total concentration of the trichoderma viride is 1.7 * 10 < 8 > cfu / ml, the ratio of lanthanum to cerium in the agricultural rare earth is 2: 1, and the ratio of lanthanum to cerium in the agricultural rare earth is 2: 1. The content of the lanthanum is 335 mg / kg. The tea garden soil texture can be improved, the soil water retention and scrap capability is improved, the tea garden soil hardening condition is reduced, and the tea yield and quality can be improved; meanwhile, the organic fertilizer can be granulated through the drying granulator, and the processing time of the organic fertilizer is shortened, so that the processing efficiency of the organic fertilizer is improved, and the organic fertilizer can be conveniently produced and processed.

This invention discloses a bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers. By weight, the bio-fertilizer comprises the following raw materials: 30-40 parts of well-rotted livestock and poultry manure, 20-30 parts of straw or rice husks, 1-2 parts of borax, 2-3 parts of urea, 2-4 parts of ammonium sulfate, 2-3 parts of zinc sulfate, 2-3 parts of magnesium sulfate, 1-2 parts of humic acid, and 1-2 parts of a microbial compound agent. The microbial compound agent comprises 4-6 parts of Bacillus megaterium, 2-3 parts of Bacillus polymyxa, 2-4 parts of Trichoderma echinococcus, 2-3 parts of Trichoderma viride, 3-5 parts of Pseudomonas fluorescens, 4-5 parts of Azotobacter chrysogenum, and 2-4 parts of Candida albicans. Using this bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers can significantly reduce the impact of continuous cropping obstacles, not only significantly reducing the incidence of cucumber wilt and root-knot nematodes and significantly promoting cucumber seedling growth, but also significantly increasing cucumber yield.

The invention discloses a crop straw rapid decomposition bacterial agent for sandy soil and a fermentation process thereof, and particularly relates to the field of agricultural microorganisms, and the bacterial agent is prepared by compounding trichoderma viride, bacillus pumilus, trichoderma longibrachiatum and bacillus subtilis according to the volume ratio of 1: 1: 2: 2. According to the preparation process, a solid culture medium taking straw as a main material is adopted, and mixed solid fermentation is performed through layered inoculation, staged variable temperature control, humidity regulation and intermittent ventilation, so that the final product has high viable count and strong enzyme activity, and can keep high activity in a severe environment of sandy soil; the straw decomposition process is quickly started and completed; the microbial inoculum can efficiently and rapidly decompose crop straws in sandy soil, significantly improve humification efficiency and soil fertility, effectively improve soil organic matter and nutrient content, improve soil structure and promote crop yield increase, and has important application value and market prospect.

The invention discloses a device and a method for producing methane by coupling crude enzyme liquid prepared from trichoderma viride with yeast glycerol for fermentation reinforcement of straw anaerobic fermentation. The method comprises the following steps: firstly, adding a trichoderma viride enzyme production culture medium and a trichoderma viride spore suspension into a trichoderma viride enzyme production culture tank for domestication enzyme production; mixing cellulose and water, adding the mixture into the trichoderma viride crude enzyme solution, converting into a cellulose mixed solution containing reducing sugar, and adding a yeast mixed solution after expanding culture to directionally produce glycerol in a yeast fermentation tank; finally, anaerobic digestion is carried out in an anaerobic digestion methane production tank; specific hydraulic retention time, pH value and temperature are set in a trichoderma viride enzyme production culture tank, a yeast culture tank, a yeast fermentation tank and a methane production tank, the utilization rate of cellulose is remarkably increased, the anaerobic digestion methane production rate is greatly increased, and then the methane yield is increased. The technology is low in investment cost and easy and convenient to operate, the production efficiency is effectively improved, and great application potential is shown.

A method for degradation and reuse of a discarded cellulose product, comprising the use of a recovered discarded cellulose product and the use of a mixed bacterial strain of Trichoderma viride, Trichoderma reesei, Aspergillus niger, and Trichoderma pseudokoningii to perform fermentation, thereby obtaining a cellulase crude product.

The invention belongs to the technical field of resource utilization, and particularly relates to a complex microbial inoculant for degrading fruit and grain mixed straw as well as a preparation method and application of the complex microbial inoculant. In order to improve the degradation efficiency and the composting efficiency of fruit and grain mixed straw waste generated under fruit and grain composite planting, the invention provides a complex microbial inoculant capable of efficiently and coordinately degrading complex lignocellulose in fruit and grain mixed straw, and the complex microbial inoculant contains bacillus subtilis, trichoderma viride and trametes versicolor. When the composite microbial agent is inoculated into the fruit and grain mixed straw, the cellulose degradation rate reaches 70% or above, and the organic fertilizer capable of improving the seed germination index can be efficiently produced. Therefore, the complex microbial inoculant disclosed by the invention realizes harmless and high-value utilization of wastes, and promotes the development of ecological cycle agriculture.

A method for degrading and reusing discarded cellulose products, including using recycled waste cellulose products and fermenting them with a mixed culture of Trichoderma viride, Trichoderma reesei, Aspergillus niger, and Trichoderma pseudokoningii to obtain crude cellulase products.

The invention provides a biological fermentation organic base fertilizer taking complete soybeans as a raw material and a preparation method of the biological fermentation organic base fertilizer, and relates to the technical field of organic fertilizers. 5-10 parts of calcium powder or rice hull powder; 0.1-0.2 part of a microbial fermentation agent, wherein the microbial fermentation agent is prepared from bacillus subtilis, saccharomycetes, bacillus mucilaginosus and trichoderma viride; the functional additive is prepared from ground phosphate rock and calcium lignosulphonate according to the mass ratio of (1.5 to 2.5): 1. The problem of insufficient nutrient conversion of a traditional soybean organic fertilizer is effectively solved through a complete soybean and complex microbial inoculant synergistic fermentation technology, the bacillus subtilis in the complex microbial inoculant decomposes soybean protein into free amino acid, the bacillus mucilaginosus releases available phosphorus, the trichoderma viride decomposes cellulose, and the four types of bacteria cooperate with each other, so that the nutrient conversion efficiency is improved. The nutrient content and the conversion efficiency of the organic base fertilizer are effectively improved.

The application relates to the technical field of agricultural fertilizers, and provides an amino sugar-oligosaccharide biofertilizer and a preparation method thereof. The amino sugar-oligosaccharide biofertilizer comprises 3-4 parts of amino sugar, 12-20 parts of seaweed oligosaccharide preparation liquid, 0.5-1 part of bacillus subtilis, 1-2 parts of trichoderma viride, 1-2 parts of aspergillus niger and 70-83 parts of base material. The seaweed oligosaccharide preparation liquid contained in the amino sugar-oligosaccharide biofertilizer is prepared by an acid enzymolysis-alkaline enzymolysis-biological fermentation three-step method, the content of seaweed oligosaccharide with a stable polymerization degree of 2-5 is high, the content of salicylic acid in crops can be effectively improved, the immune induction effect is obvious, the biocontrol and growth promotion effects of amino sugar and microorganisms are combined, the defense ability of crops against diseases is improved, and especially the growth and reproduction of pathogenic bacteria Pseudomonas syringae pv. tomato can be effectively inhibited, and the occurrence rate of bacterial spot disease of tomatoes can be reduced.