759 results about "Trichoderma viride" patented technology

The invention discloses a functional microbial fertilizer and a production method thereof. The production method comprises the following steps: grinding 60%-70% of mushroom residue and 15%-25% of livestock manure to 2-3mm to obtain the major material by a grinder, regulating water content to be 60%-65%; adding composite fermenting agent consisting of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride in accordance with 50g-100g/T; stacking, fermenting and maturing for 15-30d, and turning 5-10 times during the process; evenly mixing the major material prepared from fermentation with auxiliary materials consisting of 6%-10% humic acid and 6%-10% rice hull; adding arbuscular mycorrhiza agent in accordance with 50g-100g/T; and sieving with a 3mm sieve to obtain the fertilizer. The invention provides an economic and effective technological approach for the resource recycling of agricultural wastes in China, and has important application prospect on promoting the development of green agriculture, controlling and reducing rural environmental pollution, improving rural eco-environment and other aspects.

The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g/T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W/W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g/T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.

The invention relates to a decomposition maturing agent for degrading straw. The agent comprises active ingredients, such as candida tropicalis, aspergillus oryzae, trichoderma viride and bacillus subtilis. The decomposition maturing agent can rapidly decompose wheat straw, rice straw, grain straw, sweet potato stems, broad bean stems, rape stalks, weeds, leaves and household garbage of which the fibrous matter content is high so as to prepare an organic fertilizer. The decomposition maturing agent has the advantages of short degradation time and capability of effectively killing diseases and pests and providing nutrients for crops.

The invention discloses a trichoderma viride fungi strain and a method for preparing a bio-control fungicide thereof and application. The strain is named as trichoderma viride strain ZBS6 and collected in the CGMCC, and the collection number is CGMCC No.3538. The microbial fungicide is liquid or solid fungicide, and is prepared from the trichoderma viride ZBS6 strain. The ZBS6 strain and the fungicide thereof have wide bacteriostatic spectrum and good bacteriostatic effect, and have strong inhibiting effect on botrytis cirerea, rhizoctonia solani, rhizocto-nia cerealis, gaeumannomyces grami, marssonina coronaria, fusarium oxysporum and the like; and the fungicide can be used for control of botrytis cirerea, cucumber downy mildew, rhizocto-nia cerealis, wheat soil-borne disease and wheat powdery mildew, is efficient, non-toxic and simple to use, and does not cause environmental pollution.

The invention relates to a compound microbial organic fertilizer and a preparation method thereof. The compound microbial organic fertilizer is prepared from the following ingredients in parts by weight: 1-4 parts of trichoderma viride JUWEI002 fermentation liquor, 1-4 parts of bacillus subtilis JUWEI001 fermentation liquor, 20-30 parts of turfy soil, 33-38 parts of tobacco residue, 33-38 parts of silkworm faeces, 6-7 parts of calcium powder and 1-2 parts of potassium fulvate. The compound microbial organic fertilizer disclosed by the invention has the advantages that the quality of crops can be remarkably improved, the yield of the crops is increased, and the soil structure is improved.

The invention discloses bacillus amyloliquefaciens and the microbial inoculum and application thereof. Bacillus amyloliquefaciens is a Latin name, the strain number is HC200, and the accession number in the China General Microbiological Culture Collection Center is CGMCC No.10371. The bacillus amyloliquefaciens has a selective antagonism effect on biocontrol bacteria and pathogenic bacteria and has a strong inhibiting effect on fusarium oxysporum, fusarium solani, phytophthora nicotianae and ralstonia solanacearum, and has a good synergetic antagonism effect on pathogenic bacteria by being matched with trichoderma viride H06.

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention discloses a nano silver antibacterial latex pillow or mattress and a preparation method thereof. Tiny air holes are fully distributed on the surface of the nano silver antibacterial latex pillow or mattress, and air is filled into the tiny air holes; and the silver antibacterial latex pillow or mattress has bacterial resistance on aspergillus niger, aspergillus flavus, discolored aspergillus, penicillium citrinum, paecilomyces varioti, herbarium cladosporium, trichoderma viride and chaetomium globasum. The preparation method comprises the following steps of: uniformly mixing nano sliver with natural latex; then respectively adding sulphur, bi(triethoxy propylsilane)tetrasulfide and 2,6-butylethylene-4-methylphenol; uniformly stirring; adding soap and air to generate foams; then adding gelatin; and compressing for moulding. The nano silver antibacterial latex pillow or mattress of the invention combines air permeability, high elasticity and soft touch feeling of the latex with efficient bacterial resistance and broad spectrum sterilizing performance of nano silver and can effectively overcome the defects of easy bacterial generation, difficult air permeation, and the like of a common pillow on the market.

The invention discloses a Trichoderma viride F4 strain, and applications thereof. The Trichoderma viride F4 strain is stored at China General Microbiological Culture Collection Center on 10th November 2008, and preservation number is CGMCC No.2736. When the bacterial strain is used for producing cellulase taking pretreated lignocellulose as a carbon source and an inducer, the bacterial strain is capable of tolerating a plurality of inhibitors brought by pretreated lignocellulose, and producing enzyme with high efficiency; the secreted cellulase is comprehensive in composition, enzyme activity is high, and enzymolysis adaptability on pretreated lignocellulose substrate is high.

The invention discloses a multifunctional high efficient organic material decomposition agent and a preparation method thereof. The decomposition agent of the invention comprises composite original strains consisting of bacillus amyloliquefaciens, Aspergillus oryzae, Trichoderma viride, and Saccharomyces cerevisiae and an organic material carrier, and an inorganic carrier. Strains of the decomposition agent can be mutualistic, and have complementary advantages. The adaptation is wide, and a plurality of organic matter clastic enzymes can be produced by metabolism. The decomposition agent has high activity and can be used for fermentation and conversion of a plurality of organic materials such as livestock and poultry excrements, crop straws, city sludge, food waste, food processing and pharmaceutical waste residues, and has fast fermentation start, fast deodorization, low temperature resistance, and low moisture resistance. The fermentation period is shorter by 5-7 days than that in the same technology. Fermented and converted organic fertilizers have the obvious rhizosphere ecological regulation and control function, promote the root growth, and improve the root vitality. A plurality of soil borne diseases are prevented by improving the flora diversity, hyperparasitism and biological antagonism.

The invention relates to a method for producing a microbial feed additive by using potato vinasse. The method comprises the following steps of: preparing a vinasse culture medium; mixing bacillus subtilis, trichoderma koningii and bacterial suspension liquid of trichoderma viride spores or mouldy bran, and adding into the vinasse culture medium for culturing and fermenting; and inoculating candida tropicalis or candida utilis seed liquid in the well-fermented culture medium for continuously culturing and fermenting; and finally drying to obtain the microbial feed additive. The invention has the advantages of low energy consumption, low cost, short production period, high production efficiency and recycled resources, reduces the emission of pollutants and is beneficial to building an environment-friendly and resource-saving society; and the produced additive has wide application range, can be added into animal feeds and is a high-quality and highly-efficient microbial fermentation product.

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.

The invention provides a high-efficiency biological liquid fertilizer taking livestock liquid dung as a raw material and a preparation method of the fertilizer, and belongs to the technical field of agricultural production. The high-efficiency biological liquid fertilizer comprises the following components in parts by weight: 1 part of diluted liquid dung, 0.06-0.2 part of bran and 0.1-0.15 part of a composite microbial agent, wherein the diluted liquid dung is prepared by diluting the livestock liquid dung by water according to the weight ratio of 1:4 to 1:6; the composite microbial agent is prepared by mixing strains and EM stoste according to the weight ratio of 5:6 to 7:3; and the strains comprise two or three of trichoderma viride, saccharomyces cerevisiae and bacillus subtilis. The preparation method for the high-efficiency biological liquid fertilizer comprises the following steps of: adding the diluted livestock dung into the bran and the composite microbial agent, and performing anaerobic fermentation, filtration and nitrogen, phosphorus and potassium nutritious component mixing on the mixture, thus preparing the biological liquid fertilizer. The liquid fertilizer preparation method is high in fermentation speed, short in preparation period and high in fermentation degree; the high-efficiency biological liquid fertilizer can be obtained; the effect of increasing the yield of crops is obvious; the livestock liquid dung is effectively used in a recycled manner; and the pollution to the environment is reduced.

The invention discloses pig-chicken excrement fermenting compound bacteria, a preparation method and a method of utilizing the compound bacteria to ferment and prepare an organic fertilizer. The fermenting compound bacteria comprise the following raw material bacteria: bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus megatherium and trichoderma viride. The method for preparing the organic fertilizer comprises the following steps: mixing pig-chicken excrement with crushed straws, adding the fermenting compound bacteria to the mixture according to a dosage of 1-2 Kg per ton, mixing uniformly, the moisture being 50-65 wt%, stacking the mixed raw materials, keeping the temperature at 55-65 DEG C to conduct fermentation, two days after the fermentation, turning the stacked materials for the first time, and then turning the materials once every one or two days, keeping the moisture at 50-65 wt% during the fermentation phase, and after fermentation, obtaining the organic fertilizer with a moisture content of 20-30 wt%. The pig-chicken excrement fermenting compound bacteria disclosed by the invention can be used for fermenting the pig and chicken excrement; compared to the prior art, the method for preparing the organic fertilizer is low in productive investment and production cost, convenient to use, free of odor, and good in the fermentation and decomposing effect.

The invention provides an organic material decomposition agent, a biological organic fertilizer containing tobacco wastes, and a production method and application method of the biological organic fertilizer, wherein the organic material decomposition agent comprises the following raw materials in parts by weight: 15-20 parts of bacillus subtilis, 10-15 parts of aspergillus oryzae, 10-15 parts of aspergillus niger, 10-15 parts of trichoderma viride, 10-15 parts of bacillus mucilaginosus, 10-15 parts of saccharomyces cerevisiae, 5 parts of bacillus thermophilus, 5 parts of arthrobacter nicotinovorans and 10-25 parts of actinomycetes. The organic material decomposition agent of the tobacco wastes provided by the invention can be applied to decomposing organic matters in the tobacco wastes, so that nitrogen, phosphorus and potassium elements are degraded into the form which can be absorbed and utilized by a plant, and meanwhile, the nicotine content in the tobacco can be reduced, so as to reduce the damage to a farmland after tobacco wastes are returned to the farmland, so that the organic material decomposition agent can play a role of fertilizing the soil after being returned to the farmland.

The invention discloses a biological organic fertilizer containing compound bacteria and a preparation method. The method comprises the following steps: mixing a raw material containing an organic matter and plant ash to prepare a material to be fermented; dissolving cellulase and EM bacteria liquid in water to prepare a material fermentation spray liquid; uniformly spraying the fermentation spray liquid onto the material to be fermented, performing anaerobic fermentation for 6-7 days and aerobic fermentation for 15-20 days; then granulating the fermented and decomposed raw material, sprayingplant oil on the fertilizer particles in a coating machine, finally uniformly mixing Trichoderma harzianum chlamydospore powder, Trichoderma viride chlamydospore powder and a powder form bacterium powder carrier in a feed mixer and sending them in the coating machine, and coating the powder mixture above on the particles coated with plant oil. Compared with the prior art, the fertilizer has the advantages of thoroughly decomposing of the raw material, large hardness of the fertilizer particles, less impurity bacterium, high bacterium survival rate, comprehensive nutrition, and obvious diseases prevention and treatment effects to the crops as well as production and efficiency enhancement performance.

The invention relates to a straw fermenting agent for raising pigs, which is a composite preparation of a plurality of enzyme preparations, microorganisms and Chinese herbal medicines. The straw feed obtained by fermenting the product of the invention is added in the sow feed in a process of raising the sow, the farrowing rate of the sow and the survival rate of piglets are remarkably enhanced, and the morbidity of the sow and the piglets is reduced; and 10 percent of straw fermenting agent is added in the growing and fattening pig feed, the morbidity of the growing and fattening pigs is reduced, the slaughtering time of the growing and fattening pigs is shortened, and the utilization rate of the feed is improved. The straw fermenting agent for raising the pigs comprises the following raw materials in percent by weight: 3-5 percent of neutral protease, 3-5 percent of acid protease, 5-10 percent of phytase, 5-10 percent of cellulase, 0.2-1 percent of xylanase, 1-3 percent of pectinase, 5-10 percent of amylase, 3-10 percent of saccharomyces cerevisiae, 15-20 percent of aspergillus niger, 5-10 percent of Listeria trichoderma viride,15-20 percent of cordate houttuynia and 20-25 percent of astragalus root.

The invention relates to a microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as a preparation method and an application of the microbial agent. The microbial agent comprises the following effective components: mycobacterium gilvum CP13, trichoderma viride and rhamnolipid. The preparation method comprises the following steps: mixing bacterial solutions obtained by respectively culturing mycobacterium gilvum CP13 and trichoderma viride, simultaneously adding a composite carrier of diatomaceous earth and modified peanut shells and rhamnolipid and carrying out microbial immobilization to prepare the microbial agent. The microbial agent has super strong polycyclic aromatic hydrocarbons removal capability and meanwhile has the advantages of low cost and long shelf life; and by virtue of the microbial agent, over 90% of polycyclic aromatic hydrocarbons, such as high-concentration (50mg/kg) pyrene contained in the vegetable garden soil can be removed under conditions of room temperature (20 +/-5 DEG C) and pH of 4-9 within 36 days.

The invention discloses a straw-decomposing inoculant which comprises the following active components: bacillus subtilis, aspergillus niger, trichoderma viride, phanerochaete chrysosporimn and geobacillus stearothermophilus. In the straw-decomposing inoculant, the total content of effective viable bacteria is (1-2)* 10<8>/ml. With adoption of the straw-decomposing inoculant, the fertilizer prepared from straws can be rapidly degraded, and the normal compost fermentation is performed in advance for at least 15 days. When the straw organic fertilizer prepared from the straw-decomposing inoculant is used as the base fertilizer, alkali-hydrolyzale nitrogen, rapid available phosphorus, potassium and organic contents in the soil are higher than that in the soil in which straws are directly utilized and no fertilizer is applied, and the urease activity and cellulase activity in the soil are obviously higher than that in the soil in which straws are directly utilized and no fertilizer is applied.

The invention relates to a preparation for reducing soil-borne disease, promoting rooting and resisting continuous cropping. The preparation is prepared by mixing the following raw materials in parts by weight: 35-50 parts of fulvic acid, 10-15 parts of ferrous sulfate, 10-15 parts of magnesium sulfate, 5-8 parts of sodium octaborate, 7-10 parts of zinc sulfate, 20-30 parts of bacillus subtilis, 15-25 parts of bacillus cereus, 20-30 parts of trichoderma viride, 2-5 parts of complex amino acid and 0.1-0.5 part of indolebutyric acid. By using the preparation, a plurality of medium trace elements necessary to the growth of crops can be provided; by replenishing a certain quantity of beneficial bacteria into soil, the growth and reproduction of harmful bacteria are suppressed; protective barriers are formed on root systems, so that soil-borne disease is reduced to the maximum extent; beneficial microorganisms continuously secrete active substances such as auximone and amino acids, so that rooting is promoted greatly; various nutrients in soil can be activated and supplied to crops for absorbing and utilizing; moreover, by using the preparation together with an organic fertilizer, the granular structure of soil is changed, and soil hardening is eliminated. By adopting the preparation, the soil becomes loose, the number of roots is increased, bacteria are reduced, and continuous cropping can be resisted effectively.