103 results about "Trichoderma viride" patented technology

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention discloses a special fertilizer composition for high-yield cultivation of sugarcane, and belongs to the technical field of sugarcane planting. Comprising a disease-resistant fertilizer and an insect-resistant fertilizer; wherein the disease-resistant fertilizer comprises the following raw materials in parts by mass: 50 parts of diatomite, 5-10 parts of biomass charcoal, 1-2 parts of a compound microbial agent, 150-170 parts of an organic fertilizer and 50-60 parts of an inorganic fertilizer; wherein the compound microorganism bacterium agent comprises a bacillus subtilis bacterium agent, a trichoderma harzianum bacterium agent, a trichoderma viride bacterium agent and a paecilomyces lilacinus bacterium agent; the insect prevention fertilizer comprises beauveria bassiana suspension and matrine in a volume ratio of (3.5-4.5): 1. According to the fertilizer composition special for high-yield cultivation of sugarcane, the synergistic effect of microorganisms is utilized, the broad-spectrum inhibition effect on microorganisms in sugarcane continuous cropping disease soil can be achieved, the kieselguhr and the biomass charcoal cooperate to form a porous substance, nutrient elements are adsorbed in pores, the slow-release effect is improved, the fertilizer efficiency is prolonged, and the effect is good. Moreover, the soil structure can be improved, and the disease resistance of the sugarcane during continuous cropping is greatly improved through the synergistic interaction with the microbial complex microbial inoculants.

The invention relates to a pre-fermentation method for improving biogas production of straws and a biogas production fermentation system. The pre-fermentation method comprises the following steps: S1, preparing a mixed microbial agent to be fermented; the mixed microbial agent is prepared by inoculating the following zymophyte in parts by weight into a fermentation culture medium according to the total inoculation amount of 3-10% (v / v): 5-50 parts of bacillus subtilis, 10-20 parts of white-rot fungi, 5-10 parts of aspergillus oryzae, 5-10 parts of trametes versicolor and 5-10 parts of trichoderma viride; s2, uniformly mixing the mixed microbial agent with the micro-nano bubble water, and fermenting to form an enhanced hydrolysis bacterium solution containing a microbial agent fermentation solution and an enzyme solution; s3, spraying enhanced hydrolysis bacteria liquid onto the straws for enhanced hydrolysis so as to complete pretreatment before fermentation and biogas production; wherein the volume of the reinforced hydrolytic bacteria liquid required by each kg of straw is 3-5L. Compared with the prior art, the mixed microbial agent and the micro-nano bubble water are used for carrying out cooperative treatment on the straw, so that the gas production efficiency and the methane production amount of the straw can be effectively improved.

The invention relates to a trichoderma virens strain and application thereof. According to the invention, a Trichoderma virens strain GZUAS831026 is separated from a capsicum land soil sample, the Trichoderma virens strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the Trichoderma virens strain is GDMCC No: 65370. The trichoderma virens strain obtained by the invention has a good pepper straw degradation effect and a growth promoting effect, has a good inhibition effect on pathogenic bacteria of the ginger tuber rot disease, and can be used for preventing and treating the ginger tuber rot disease.

The invention discloses a trichoderma virens preparation capable of preventing diseases and increasing yield as well as a preparation method and application of the trichoderma virens preparation. Belongs to the technical field of biological agents. According to the trichoderma virens strain T259 disclosed by the invention, the trichoderma virens strain T259 is respectively subjected to strain amplification culture, liquid amplification culture and solid fermentation culture, a solid fermentation product of the T259 strain is successfully prepared, the solid fermentation product is mixed with an auxiliary agent, and the trichoderma virens preparation capable of preventing diseases and increasing yield is prepared. The trichoderma virens spore content in the preparation is improved; solid fermentation can effectively improve the colonization ability of trichoderma virens in the field. The production of the trichoderma virens preparation has the characteristics of large sporulation quantity, short period, wide raw material source and low cost; the fertilizer is easy to use and operate, suitable for various crops and remarkable in disease prevention and yield increase effect. And a foundation is laid for quality and efficiency improvement and high-quality and high-efficiency development of modern agriculture.

The invention relates to the field of bactericides, and particularly discloses a microbial bactericide and a preparation method thereof. The microbial bactericide is prepared from the following raw material components: 40 to 45 parts of microbial source fermentation liquor; 50 to 60 parts of a carrier; 2-3 parts of a dispersing aid; 3-5 parts of a surfactant; 0.5 to 1 part of a synergist; microbial sources in the microbial source fermentation liquor comprise bacillus, trichoderma harzianum, trichoderma viride and pythium oligandrum according to the weight ratio of 50: (20-25): (10-15): 10, and the carrier comprises at least two of sodium lactate, calcium sulfate and manganese sulfate; the preparation method comprises the following steps: I, activating and culturing a microbial source; iI, preparing microbial source fermentation liquor; and III, preparing the microbial bactericide. The composition disclosed by the invention has high bactericidal ability, high stability and long-acting bactericidal ability, the prevention and treatment effect can be not lower than 71.5%, and the suspension rate is not lower than 97.85%.

The invention relates to the technical field of soil improvement, in particular to a soil conditioner for improving desert saline-alkali soil and application of the soil conditioner. The soil conditioner is prepared from 5.5 to 7.5 * 10 < 4 > kg of yellow water, 5 to 6 * 10 < 3 > kg of crop straw, 60 to 76 kg of phanerochaete chrysosporium, 50 to 60 kg of bacillus amyloliquefaciens J10, 30 to 46 kg of trichoderma viride, 30 to 50 kg of lactobacillus kefir CP423, 1 to 1.2 * 10 < 4 > kg of organic fertilizer, 0.8 to 1.2 * 10 < 3 > kg of amino acid water-soluble fertilizer, 25 to 35 kg of nitrogen fertilizer and 8 to 12 kg of phosphate fertilizer. After the soil conditioner is applied to ecological improvement, the content of organic matter, total nitrogen, total phosphorus, alkali-hydrolyzable nitrogen and available phosphorus can be effectively increased, the soil quality is remarkably improved, and the yield and quality of planted crops can be remarkably improved.

The application provides a solid substrate for soilless culture prepared by fermentation of coffee residue and mixed bacteria, wherein the mixed bacteria include yeast, bacillus subtilis and trichoderma viride; the weight ratio of the yeast, the bacillus subtilis and the trichoderma viride is 2:1:3. The solid substrate for soilless culture is rich in trace elements required by plant growth, has low bulk density, high water holding porosity, and is controllable. The solid substrate for soilless culture can effectively improve the plant growth environment, has disease resistance and insect resistance, and improves the survival rate of plants.

The invention relates to the technical field of fertilizer preparation, in particular to traditional Chinese medicine compound microbial fertilizer for inhibiting angelica sinensis root rot. The compound microbial fertilizer for inhibiting root rot of angelica sinensis comprises 45-55 parts of a compound fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomite, 4-8 parts of a compound bacterial agent and 8-12 parts of a traditional Chinese medicine extract, the complex microbial inoculant is prepared from bacillus licheniformis, bacillus cereus, bacillus atrophaeus, pseudomonas chlororaphis, pseudomonas brassicae, trichoderma viride and trichoderma harzianum according to the mass ratio of (14 to 18) to (12 to 16) to (11 to 15) to (8 to 12) to (8 to 12) to (5 to 9) to (5 to 9), and the bacterium content is 4 to 6 billion / g; the traditional Chinese medicine extract is prepared from radix scutellariae, herba ephedrae, marigold, oleander, folium ginkgo and radix sophorae flavescentis. Angelica sinensis root rot can be prevented and treated, the yield and quality of angelica sinensis can be remarkably improved, and the effect is excellent.

The present invention relates to the technical field of biological control, and particularly to a compound microbial agent for preventing and controlling plant diseases and its application. The microbial combination provided by the present invention includes Bacillus subtilis BLG010, Bacillus amyloliquefaciens HW05, Paecilomyces lilacinus E16, Trichoderma viride H06, Bacillus velezensis LG14-3 and Bacillus amyloliquefaciens HC200 which have a synergistic effect; a compound microbial agent is also provided, and the agent includes the microbial combination, a carrier and a synergist provided by the present invention, and also includes a healthy plant leaf extract or a healthy plant rhizosphere soil extract. The agent has a control effect on a variety of major plant diseases (pepper blight, banana wilt, tomato wilt and bacterial wilt, citrus huanglongbing, tea blister blight and tea target spot), and at the same time can change the fungal or bacterial diversity of plant leaves or rhizosphere soil, as well as increase the abundance of beneficial microorganisms or reduce pathogenic microorganisms.

A biocontrol formulation for plant pathogens includes a Trichoderma viride filtrate and an inert substance selected from the group consisting of a viscosity reducer, a surfactant, flour, clay, and combinations thereof. In an embodiment, the filtrate includes conidia of the Trichoderma viride. In an embodiment, the filtrate includes byproducts obtained from the fermentation of Trichoderma viride. In an embodiment, the byproducts include at least one of the organic acids, growth regulators, and amino acids.

The invention relates to the technical field of solid waste resourceful treatment, and discloses coal gangue-based improved soil and a preparation method thereof. The coal gangue-based improved soil comprises the following raw materials: coal gangue, sandy soil and a fermentation inoculant, the fermentation bacterial agent is prepared from bacillus subtilis SHBCC D11446, arbuscular mycorrhizal fungi SHBCC D24696, trichoderma viride SHBCC D10197 and bacillus velezensis GS02, and the preservation number of the bacillus velezensis GS02 is CCTCC (China Center for Type Culture Collection) NO: M 2025531. Phosphorus, potassium, nitrogen and organic matters in the coal gangue can be fully utilized, the prepared coal gangue-based improved soil has high nutrient substance content and low effective state heavy metal content, and meanwhile, the situation that the alkalinity and the salt content of the improved soil are greatly improved due to introduction of the coal gangue can be avoided; the improved soil can effectively promote plant growth.

The present invention relates to the technical field of microbial fermentation, and in particular to a fermentation medium of green Trichoderma, a Trichoderma agent and a preparation method thereof. The present invention adopts liquid-solid combined fermentation, solid-state fermentation with millet as the main raw material, supplemented with a quick-acting carbon and nitrogen source and an inorganic salt, ferments for 48 hours, sieves green Trichoderma conidia powder, mixes the conidia powder with a wetting agent, a dispersant and a carrier, etc. to prepare green Trichoderma wettable powder, the preparation has good water solubility, can be used for spraying and drip irrigation of crops, can prevent and treat plant diseases, and has the advantages of safety and high efficiency, no drug residue, no toxic side effects, reducing the use of antibiotics, and improving crop immunity.

The invention provides an ensilage fermented feed and a preparation method thereof, which adopt a staged sequential fermentation strategy: firstly, trichoderma viride secretes cellulase under an aerobic condition to decompose fibers and release soluble sugar, and then lactobacillus rhamnosus quickly produces acid under an anaerobic environment to inhibit putrefying bacteria; the digestibility and the stability of the feed are obviously improved; meanwhile, pH-responsive nutritional microcapsules are also added into the raw materials during preparation of the ensilage fermented feed: pectin oligosaccharide, chitosan and cassava starch are used as wall materials, rosmarinic acid, calcium lactate and bacillus coagulans are embedded, and core materials are released by stages through pH responsiveness, so that the ensilage fermented feed is prepared; the nutritional value of the feed is improved, digestion and absorption are promoted, and the intestinal health of animals is improved.

According to the probiotic liquid capable of promoting agilawood formation, the lasiodiplodia theobroma, the laetiporus sulphureus and the trichoderma viride are adopted to form the probiotic liquid, the biotransformation process in a tree body is promoted, the probiotic liquid can shorten the agilawood formation time, and the content of extracts in agilawood is high.

This invention provides a *Trichoderma viride* RM9 strain antagonizing jasmine white mold disease and its application. The *Trichoderma viride* RM9 strain of this invention is protected under the accession number CCTCC M 20221442. *Trichoderma viride* RM9 of this invention is isolated from the rhizosphere of jasmine plants, exhibits strong rhizosphere colonization ability, and demonstrates good control efficacy against jasmine white mold disease, achieving a control effect of 83.34%. It also promotes the growth of jasmine seedlings. Furthermore, its cultivation conditions are relatively simple, and its development into a solid inoculant is beneficial for improving jasmine yield and economic benefits.

The present invention belongs to the technical field of crop control, and discloses a compound microbial agent containing Bacillus pumilus and Trichoderma viride and its application in biological control of citrus. The Bacillus pumilus is Bacillus pumilus ( Bacillus pumilus ), HBTG2356, and its preservation number is: CCTCC NO: M20232119. The Trichoderma viride is Trichoderma viride ( Trichoderma virens ), HBTG3206, and its preservation number is: CCTCC NO: M20232117. Compared with single strains, this compound strain has a synergistic effect on diseases such as citrus canker and citrus root-knot nematode, and has a certain application prospect in the prevention and control of citrus diseases.

The invention relates to the field of multi-enzyme organic fertilizers, and discloses a biological bacterium fermentation multi-enzyme organic fertilizer and a preparation method of the biological bacterium fermentation multi-enzyme organic fertilizer. 12 to 20 parts of soybean meal; 5-10 parts of a humic acid raw material; 3-8 parts of a mineral additive; 2-5 parts of sugar auxiliary materials; 0.5 to 1.5 parts of a functional microbial agent; 1.0 to 2.5 parts of trichoderma viride powder; 0.8 to 1.5 parts of sodium alginate; and 1.5 to 3.0 parts of xylooligosaccharide. The trichoderma viride powder is utilized to secrete cellulase, xylanase and protease, organic waste such as straw and soybean meal is effectively degraded and converted into nutrients capable of being absorbed by crops, meanwhile, plant hormones secreted by the trichoderma viride can stimulate root development, promote branch extension, enhance nutrient absorption and improve the growth vigor of the crops, and the yield of the crops is increased. The effectiveness of phosphorus in the soil and the fertilizer utilization efficiency are obviously improved.

The invention relates to a method for separating and purifying active substances in trichoderma citrinoviride fermentation liquor, in particular to a method for separating and purifying trichoderma citrinoviride fermentation liquor. Comprising the steps of extraction of trichoderma citrinoviride fermentation liquor and purification and analysis of active substances in the trichoderma citrinoviride fermentation liquor, the trichoderma citrinoviride fermentation liquor is subjected to ethyl acetate extraction and vacuum concentration to obtain a crude extract, an efficient preparative liquid phase is adopted for separation and purification in the purification and analysis step, and methanol and formic acid water are selected in the purification and analysis step. Through the steps of extracting the trichoderma citrinoviride fermentation liquor and purifying and analyzing the active substances in the trichoderma citrinoviride fermentation liquor, the separation and purification process of the trichoderma citrinoviride fermentation liquor is simple, the cost is low, the operation is simple and convenient, and the product purity is high.

The invention discloses a bio-based fertilizer and application thereof. The preparation method comprises the following steps: S1, crushing the mango pomace and the bagasse, sieving the crushed mango pomace and bagasse with a 40-60-mesh sieve, adding diluted hydrochloric acid and ferrous sulfate, mixing the crushed mango pomace and bagasse, carrying out a hydrolysis reaction, and filtering a hydrolysis product to obtain solid residues; s2, seed liquid of bacillus coagulans, bacillus pseudomycoides, trichoderma viride and saccharomyces cerevisiae is inoculated into the mixture of the solid residues and the cattle and sheep manure for fermentation, and a fermentation product is obtained; s3, mixing the fermentation product, charcoal and polyglutamic acid to prepare the fertilizer. The mango pomace, solid bagasse and cattle and sheep manure mixture are cooperatively fermented through compound bacteria, the porosity of the fertilizer is increased, the water and fertilizer retention capacity is improved, the fermentation product, biochar and polyglutamic acid are mixed and granulated to prepare the fertilizer, the disintegration rate can be regulated and controlled, nutrients are prevented from being instantly released, and root growth and microorganism colonization are promoted.

The present invention relates to the field of microbial fermentation technology, and in particular to a strain of Paraphoma chlamydocopiosa and a method for degrading cellulose in Chinese medicine residues, and a composite bacteria and a method for degrading cellulose in Chinese medicine residues. The present invention provides a strain of Paraphoma chlamydocopiosa AM‑PC, which is deposited in the Institute of Microbiology, Chinese Academy of Sciences, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit date of October 9, 2023 and a deposit number of CGMCC No. 40893. The Paraphoma chlamydocopiosa AM‑PC of the present invention is combined with Trichoderma viride to produce cellulase using Chinese medicine residues as a culture medium, has high activity, and can degrade cellulose in Chinese medicine residues.

The invention belongs to the field of plasmid vector construction, and provides a plasmid vector as well as a construction method and application thereof. The plasmid vector is constructed by modifying pAN7-1, a trichoderma viride promoter gene and a resistance gene are inserted into multiple cloning sites of the pAN7-1 of the plasmid vector, and the resistance gene is located at the downstream of the trichoderma viride promoter gene. According to the invention, the selected trichoderma viride promoter gene segment can efficiently start the gene connected behind, so that the transformation efficiency of the plasmid vector is greatly improved. The invention establishes a method for efficiently transforming trichoderma viride by using an agrobacterium tumefaciens-mediated exogenous gene, and can provide help in the aspects of genetic modification of trichoderma viride, development of useful genes, separation of drug target genes and the like.

The invention provides a trichoderma preparation for improving the yield and quality of sugarcane as well as a preparation method and application of the trichoderma preparation, and belongs to the technical field of microbial preparations. The trichoderma preparation is prepared from the following components in parts by mass: 15-25 parts of trichoderma viride, 10-20 parts of trichoderma koningii and 2-4 parts of a seaweed extract. The trichoderma viride, the trichoderma koningii and the seaweed extract can achieve a synergistic effect, and absorption and utilization of the sugarcane on nutrient substances are promoted. The trichoderma preparation is sprayed once in a sugarcane seedling stage and a stem node elongation stage respectively, so that the yield and the quality of the sugarcane can be remarkably improved.

The invention belongs to the technical field of fertilizers. The invention provides a waste fruit fermentation compound fertilizer and a preparation method thereof.The preparation method comprises the steps that S1, 54% by mass of walnut green seedcase, 33-35% by mass of edible fungus residues and 11-13% by mass of plant ash are mixed to be uniform, and a basic material is obtained; s2, adding a functional component accounting for 8.5-10% of the mass of the basic material into the basic material, and uniformly mixing to obtain a fermentation base material; the functional components comprise dolomite powder and rice hull ash; s3, adding 0.1-0.12% by mass of a compound fermentation inoculant into the fermentation base material, uniformly mixing, and then composting and fermenting to obtain the waste fruit fermentation compound fertilizer, wherein the mass of the compound fermentation inoculant is 0.1-0.12% of that of the fermentation base material; the compound fermentation inoculant is prepared by uniformly mixing mixed bacteria and wheat bran according to a mass ratio of 1: (4-4.5); the mixed bacteria comprise trichoderma viride, bacillus subtilis, aspergillus oryzae, aspergillus niger, saccharomycetes and lactic acid bacteria. The fertilizer efficiency of the compound fertilizer prepared from the waste fruit / abandoned fruit walnut green seedcase as the raw material is effectively improved, and the yield of the pakchoi is further improved.

The utility model relates to the technical field of mixing devices, and discloses a mixing device for producing a trichoderma viride biocontrol inoculant. The mixing device for producing the trichoderma viride biocontrol inoculant comprises a tank body; the hopper is positioned at the bottom of the tank body and is used for crushing the blocky trichoderma viride biocontrol microbial inoculum; a rotating column is arranged in the tank body, the top of the rotating column is connected with the tank body through a mechanical seal, the rotating column is connected with the inner side of a spiral piece through a fastener, an elastic piece pushes a scraper blade to be attached to the bottom of a hopper through a telescopic column, and the hopper rotates to drive a blocky trichoderma viride biocontrol microbial inoculum to rotate. And the scraping plate is used for crushing the blocky trichoderma viride biocontrol microbial inoculum until the blocky trichoderma viride biocontrol microbial inoculum can fall outside through the opening in the bottom of the hopper, so that the blocky trichoderma viride biocontrol microbial inoculum can be crushed without being crushed independently, time and labor are saved, and the working efficiency is improved.

The invention provides a trichoderma inoculant, a preparation method thereof and application of the trichoderma inoculant in improving yield and quality of beet, and belongs to the technical field of agricultural microbial inoculants. The trichoderma agent comprises the following components: trichoderma harzianum, trichoderma viride, purpureocillium lilacinum, bacillus amyloliquefaciens and bacillus laterosporus in a mass ratio of (3-5): (1-3): (2-4): (2-4): (1-2). According to the invention, the strains cooperate with each other to promote the growth of beet, effectively enhance the immune resistance of beet, and effectively prevent and reduce beet root rot, brown blotch and other diseases caused by continuous cropping; the photosynthesis efficiency of the beet can be enhanced, the accumulation of photosynthetic products is improved, the soil structure is improved, the number of beneficial bacteria groups in the soil is increased, and the rhizosphere environment is improved, so that the sugar content and yield of the beet are increased. By applying the microbial agent disclosed by the invention, multiple effects of increasing the yield per mu, increasing the sugar content of beet, improving soil, improving the fertilizer efficiency, resisting diseases, preventing insects and the like can be achieved, and farmers are helped to increase both production and income.

The invention relates to the technical field of biological environmental protection, and discloses a compound microbial agent for constructing micro-ecological balance based on five florae. Comprising the following raw materials in parts by weight: 3-5 parts of photosynthetic bacteria, 1-2 parts of lactic acid bacteria, 2-4 parts of yeast acid, 2-5 parts of bacillus, 2-5 parts of actinomycetes, 1-2 parts of acetic bacteria, 2-3 parts of trichoderma viride, 2-4 parts of bifidobacterium, 2-3 parts of lactobacillus plantarum, 1-2 parts of nitrifying spina bacteria, 10-14 parts of corn, 10-14 parts of soybeans, 10-14 parts of sorghum and 1-3 parts of aspergillus. According to the compound microbial agent with micro-ecological balance constructed based on the five flora, the aspergillus is decomposed into monosaccharide by utilizing energy, the monosaccharide becomes food of saccharomycetes, the monosaccharide is converted into alcohol after being subjected to saccharomycetes bacterial decomposition, the alcohol is converted into acetic acid by acetic acid bacteria, and carbon dioxide generated by the saccharomycetes is utilized by photosynthetic bacteria to prepare glucose; the microbiota complement each other to live together, and micro-ecological balance is constructed through the five microbiota.

The present invention relates to a green Trichoderma mutant strain with high beta-glucanase production, belonging to the field of microbial technology. The present invention provides a green Trichoderma (Trichoderma viride) UN-101 strain with high beta-glucanase production, with a deposit number of CGMCC NO.40414. The present invention also discloses an industrial production method for producing beta-glucanase using the strain, and the fermentation broth enzyme activity can reach more than 53000U / mL by seed culture and liquid deep fermentation with the strain, and the beta-glucanase product obtained by extraction has low finished product cost, high beta-glucanase enzyme activity, good stability and strong specificity. The invention is characterized in that the optimum reaction temperature is 45°C, and the enzyme activity can still be maintained at more than 85% when kept at 80°C for 2 hours, and the thermal stability is good; the optimum reaction pH is 3.0, and the enzyme can maintain more than 80% of its activity when treated in the pH range of 2.0-6.5 for 2 hours, and is an acidic glucanase; the invention has excellent resistance to hydrolysis by pepsin, trypsin and chymosin.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The application provides a degradable soilless and trayless seedling raising blanket, which is in a plate structure and comprises a biological base material and a biological binder; the biological binder is obtained by fermentation of a lees enzymolysis solution and a synergist after inoculation of a strain; the lees enzymolysis solution is prepared from fresh lees and glucoamylase; the synergist is prepared from natural herbs, white sugar, deionized water and a bacterial solution according to a mass ratio of 1:15:150:15; the bacterial solution comprises Trichoderma viride JX18-0201; the Trichoderma viride JX18-0201 has a preservation number of CCTCC NO:M2021509. The technical principle of the application is that the blocky seedling raising blanket formed by the biological base material and the biological binder replaces the original traditional seedling raising substrate and plastic seedling tray, can meet the nutrition of normal seedling emergence and development of plants, has the characteristics of the plastic seedling tray, can be directly used for seedling transplanting, and does not need to use the plastic seedling tray, thereby reducing the cost of recycling and processing.