54 results about "Trichoderma viride" patented technology

The invention discloses a trichoderma virens preparation capable of preventing diseases and increasing yield as well as a preparation method and application of the trichoderma virens preparation. Belongs to the technical field of biological agents. According to the trichoderma virens strain T259 disclosed by the invention, the trichoderma virens strain T259 is respectively subjected to strain amplification culture, liquid amplification culture and solid fermentation culture, a solid fermentation product of the T259 strain is successfully prepared, the solid fermentation product is mixed with an auxiliary agent, and the trichoderma virens preparation capable of preventing diseases and increasing yield is prepared. The trichoderma virens spore content in the preparation is improved; solid fermentation can effectively improve the colonization ability of trichoderma virens in the field. The production of the trichoderma virens preparation has the characteristics of large sporulation quantity, short period, wide raw material source and low cost; the fertilizer is easy to use and operate, suitable for various crops and remarkable in disease prevention and yield increase effect. And a foundation is laid for quality and efficiency improvement and high-quality and high-efficiency development of modern agriculture.

The application provides a solid substrate for soilless culture prepared by fermentation of coffee residue and mixed bacteria, wherein the mixed bacteria include yeast, bacillus subtilis and trichoderma viride; the weight ratio of the yeast, the bacillus subtilis and the trichoderma viride is 2:1:3. The solid substrate for soilless culture is rich in trace elements required by plant growth, has low bulk density, high water holding porosity, and is controllable. The solid substrate for soilless culture can effectively improve the plant growth environment, has disease resistance and insect resistance, and improves the survival rate of plants.

A biocontrol formulation for plant pathogens includes a Trichoderma viride filtrate and an inert substance selected from the group consisting of a viscosity reducer, a surfactant, flour, clay, and combinations thereof. In an embodiment, the filtrate includes conidia of the Trichoderma viride. In an embodiment, the filtrate includes byproducts obtained from the fermentation of Trichoderma viride. In an embodiment, the byproducts include at least one of the organic acids, growth regulators, and amino acids.

This invention provides a *Trichoderma viride* RM9 strain antagonizing jasmine white mold disease and its application. The *Trichoderma viride* RM9 strain of this invention is protected under the accession number CCTCC M 20221442. *Trichoderma viride* RM9 of this invention is isolated from the rhizosphere of jasmine plants, exhibits strong rhizosphere colonization ability, and demonstrates good control efficacy against jasmine white mold disease, achieving a control effect of 83.34%. It also promotes the growth of jasmine seedlings. Furthermore, its cultivation conditions are relatively simple, and its development into a solid inoculant is beneficial for improving jasmine yield and economic benefits.

The invention provides a trichoderma preparation for improving the yield and quality of sugarcane as well as a preparation method and application of the trichoderma preparation, and belongs to the technical field of microbial preparations. The trichoderma preparation is prepared from the following components in parts by mass: 15-25 parts of trichoderma viride, 10-20 parts of trichoderma koningii and 2-4 parts of a seaweed extract. The trichoderma viride, the trichoderma koningii and the seaweed extract can achieve a synergistic effect, and absorption and utilization of the sugarcane on nutrient substances are promoted. The trichoderma preparation is sprayed once in a sugarcane seedling stage and a stem node elongation stage respectively, so that the yield and the quality of the sugarcane can be remarkably improved.

The invention belongs to the technical field of fertilizers. The invention provides a waste fruit fermentation compound fertilizer and a preparation method thereof.The preparation method comprises the steps that S1, 54% by mass of walnut green seedcase, 33-35% by mass of edible fungus residues and 11-13% by mass of plant ash are mixed to be uniform, and a basic material is obtained; s2, adding a functional component accounting for 8.5-10% of the mass of the basic material into the basic material, and uniformly mixing to obtain a fermentation base material; the functional components comprise dolomite powder and rice hull ash; s3, adding 0.1-0.12% by mass of a compound fermentation inoculant into the fermentation base material, uniformly mixing, and then composting and fermenting to obtain the waste fruit fermentation compound fertilizer, wherein the mass of the compound fermentation inoculant is 0.1-0.12% of that of the fermentation base material; the compound fermentation inoculant is prepared by uniformly mixing mixed bacteria and wheat bran according to a mass ratio of 1: (4-4.5); the mixed bacteria comprise trichoderma viride, bacillus subtilis, aspergillus oryzae, aspergillus niger, saccharomycetes and lactic acid bacteria. The fertilizer efficiency of the compound fertilizer prepared from the waste fruit / abandoned fruit walnut green seedcase as the raw material is effectively improved, and the yield of the pakchoi is further improved.

The invention provides a trichoderma inoculant, a preparation method thereof and application of the trichoderma inoculant in improving yield and quality of beet, and belongs to the technical field of agricultural microbial inoculants. The trichoderma agent comprises the following components: trichoderma harzianum, trichoderma viride, purpureocillium lilacinum, bacillus amyloliquefaciens and bacillus laterosporus in a mass ratio of (3-5): (1-3): (2-4): (2-4): (1-2). According to the invention, the strains cooperate with each other to promote the growth of beet, effectively enhance the immune resistance of beet, and effectively prevent and reduce beet root rot, brown blotch and other diseases caused by continuous cropping; the photosynthesis efficiency of the beet can be enhanced, the accumulation of photosynthetic products is improved, the soil structure is improved, the number of beneficial bacteria groups in the soil is increased, and the rhizosphere environment is improved, so that the sugar content and yield of the beet are increased. By applying the microbial agent disclosed by the invention, multiple effects of increasing the yield per mu, increasing the sugar content of beet, improving soil, improving the fertilizer efficiency, resisting diseases, preventing insects and the like can be achieved, and farmers are helped to increase both production and income.

The invention relates to the technical field of biological environmental protection, and discloses a compound microbial agent for constructing micro-ecological balance based on five florae. Comprising the following raw materials in parts by weight: 3-5 parts of photosynthetic bacteria, 1-2 parts of lactic acid bacteria, 2-4 parts of yeast acid, 2-5 parts of bacillus, 2-5 parts of actinomycetes, 1-2 parts of acetic bacteria, 2-3 parts of trichoderma viride, 2-4 parts of bifidobacterium, 2-3 parts of lactobacillus plantarum, 1-2 parts of nitrifying spina bacteria, 10-14 parts of corn, 10-14 parts of soybeans, 10-14 parts of sorghum and 1-3 parts of aspergillus. According to the compound microbial agent with micro-ecological balance constructed based on the five flora, the aspergillus is decomposed into monosaccharide by utilizing energy, the monosaccharide becomes food of saccharomycetes, the monosaccharide is converted into alcohol after being subjected to saccharomycetes bacterial decomposition, the alcohol is converted into acetic acid by acetic acid bacteria, and carbon dioxide generated by the saccharomycetes is utilized by photosynthetic bacteria to prepare glucose; the microbiota complement each other to live together, and micro-ecological balance is constructed through the five microbiota.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The application provides a degradable soilless and trayless seedling raising blanket, which is in a plate structure and comprises a biological base material and a biological binder; the biological binder is obtained by fermentation of a lees enzymolysis solution and a synergist after inoculation of a strain; the lees enzymolysis solution is prepared from fresh lees and glucoamylase; the synergist is prepared from natural herbs, white sugar, deionized water and a bacterial solution according to a mass ratio of 1:15:150:15; the bacterial solution comprises Trichoderma viride JX18-0201; the Trichoderma viride JX18-0201 has a preservation number of CCTCC NO:M2021509. The technical principle of the application is that the blocky seedling raising blanket formed by the biological base material and the biological binder replaces the original traditional seedling raising substrate and plastic seedling tray, can meet the nutrition of normal seedling emergence and development of plants, has the characteristics of the plastic seedling tray, can be directly used for seedling transplanting, and does not need to use the plastic seedling tray, thereby reducing the cost of recycling and processing.

The invention provides a crop nutritional supplement containing glycopeptide as well as a preparation method and application of the crop nutritional supplement, and belongs to the technical field of biological agriculture. The crop nutritional supplement comprises fish protein peptide, fucose oligosaccharide, fulvic acid, silicon-calcium-potassium-magnesium fertilizer, bone meal, polyglutamic acid, a microbial agent and a carrier. Wherein the fish protein peptide is obtained by fermenting deep-sea fish meat with bacillus subtilis SH21 and performing enzymolysis with compound enzyme, and the microbial agent is prepared from trichoderma atroviride C61 and paenibacillus mucilaginosus HB-02. The crop nutritional supplement can activate the immune system of crops, enhance disease resistance, promote nutrient absorption and improve the yield and quality of crops, is environment-friendly, and can solve the problems caused by chemical pesticides and traditional chemical fertilizers.

This invention discloses a bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers. By weight, the bio-fertilizer comprises the following raw materials: 30-40 parts of well-rotted livestock and poultry manure, 20-30 parts of straw or rice husks, 1-2 parts of borax, 2-3 parts of urea, 2-4 parts of ammonium sulfate, 2-3 parts of zinc sulfate, 2-3 parts of magnesium sulfate, 1-2 parts of humic acid, and 1-2 parts of a microbial compound agent. The microbial compound agent comprises 4-6 parts of Bacillus megaterium, 2-3 parts of Bacillus polymyxa, 2-4 parts of Trichoderma echinococcus, 2-3 parts of Trichoderma viride, 3-5 parts of Pseudomonas fluorescens, 4-5 parts of Azotobacter chrysogenum, and 2-4 parts of Candida albicans. Using this bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers can significantly reduce the impact of continuous cropping obstacles, not only significantly reducing the incidence of cucumber wilt and root-knot nematodes and significantly promoting cucumber seedling growth, but also significantly increasing cucumber yield.

The invention discloses a crop straw rapid decomposition bacterial agent for sandy soil and a fermentation process thereof, and particularly relates to the field of agricultural microorganisms, and the bacterial agent is prepared by compounding trichoderma viride, bacillus pumilus, trichoderma longibrachiatum and bacillus subtilis according to the volume ratio of 1: 1: 2: 2. According to the preparation process, a solid culture medium taking straw as a main material is adopted, and mixed solid fermentation is performed through layered inoculation, staged variable temperature control, humidity regulation and intermittent ventilation, so that the final product has high viable count and strong enzyme activity, and can keep high activity in a severe environment of sandy soil; the straw decomposition process is quickly started and completed; the microbial inoculum can efficiently and rapidly decompose crop straws in sandy soil, significantly improve humification efficiency and soil fertility, effectively improve soil organic matter and nutrient content, improve soil structure and promote crop yield increase, and has important application value and market prospect.

The invention belongs to the technical field of resource utilization, and particularly relates to a complex microbial inoculant for degrading fruit and grain mixed straw as well as a preparation method and application of the complex microbial inoculant. In order to improve the degradation efficiency and the composting efficiency of fruit and grain mixed straw waste generated under fruit and grain composite planting, the invention provides a complex microbial inoculant capable of efficiently and coordinately degrading complex lignocellulose in fruit and grain mixed straw, and the complex microbial inoculant contains bacillus subtilis, trichoderma viride and trametes versicolor. When the composite microbial agent is inoculated into the fruit and grain mixed straw, the cellulose degradation rate reaches 70% or above, and the organic fertilizer capable of improving the seed germination index can be efficiently produced. Therefore, the complex microbial inoculant disclosed by the invention realizes harmless and high-value utilization of wastes, and promotes the development of ecological cycle agriculture.

A method for degrading and reusing discarded cellulose products, including using recycled waste cellulose products and fermenting them with a mixed culture of Trichoderma viride, Trichoderma reesei, Aspergillus niger, and Trichoderma pseudokoningii to obtain crude cellulase products.

The invention provides a biological fermentation organic base fertilizer taking complete soybeans as a raw material and a preparation method of the biological fermentation organic base fertilizer, and relates to the technical field of organic fertilizers. 5-10 parts of calcium powder or rice hull powder; 0.1-0.2 part of a microbial fermentation agent, wherein the microbial fermentation agent is prepared from bacillus subtilis, saccharomycetes, bacillus mucilaginosus and trichoderma viride; the functional additive is prepared from ground phosphate rock and calcium lignosulphonate according to the mass ratio of (1.5 to 2.5): 1. The problem of insufficient nutrient conversion of a traditional soybean organic fertilizer is effectively solved through a complete soybean and complex microbial inoculant synergistic fermentation technology, the bacillus subtilis in the complex microbial inoculant decomposes soybean protein into free amino acid, the bacillus mucilaginosus releases available phosphorus, the trichoderma viride decomposes cellulose, and the four types of bacteria cooperate with each other, so that the nutrient conversion efficiency is improved. The nutrient content and the conversion efficiency of the organic base fertilizer are effectively improved.

The application relates to the technical field of agricultural fertilizers, and provides an amino sugar-oligosaccharide biofertilizer and a preparation method thereof. The amino sugar-oligosaccharide biofertilizer comprises 3-4 parts of amino sugar, 12-20 parts of seaweed oligosaccharide preparation liquid, 0.5-1 part of bacillus subtilis, 1-2 parts of trichoderma viride, 1-2 parts of aspergillus niger and 70-83 parts of base material. The seaweed oligosaccharide preparation liquid contained in the amino sugar-oligosaccharide biofertilizer is prepared by an acid enzymolysis-alkaline enzymolysis-biological fermentation three-step method, the content of seaweed oligosaccharide with a stable polymerization degree of 2-5 is high, the content of salicylic acid in crops can be effectively improved, the immune induction effect is obvious, the biocontrol and growth promotion effects of amino sugar and microorganisms are combined, the defense ability of crops against diseases is improved, and especially the growth and reproduction of pathogenic bacteria Pseudomonas syringae pv. tomato can be effectively inhibited, and the occurrence rate of bacterial spot disease of tomatoes can be reduced.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for producing cellulose by adopting trichoderma viride, an enzyme produced by the method and application of the enzyme. The bacterial strain is Trichoderma viride LKT2025-06, and the preservation number of the Trichoderma viride LKT2025-06 is CGMCC (China General Microbiological Culture Collection Center) No. 42215. The enzyme activity level of cellulase filter paper produced through industrial fermentation of the strain can reach 750 U / mL or above, high-activity cellulase can be generated, a certain amount of xylanase and mannanase can also be generated, hemicellulose is decomposed while cellulose is degraded, more comprehensive and efficient degradation of biomass is achieved, and the production cost is reduced. The utilization efficiency of biomass resources is improved.

The invention discloses a trichoderma viride strain and application thereof. The strain is a trichoderma viride strain FS50, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 41855. The trichoderma viride strain FS50 has an obvious inhibiting effect on various grape root rot pathogenic bacteria and has the characteristic of broad-spectrum bacteriostasis, a strain fermentation filtrate can obviously inhibit the growth of the root rot pathogenic bacteria and has good bacteriostatic activity, and volatile substances and non-volatile metabolites generated by the strain can effectively inhibit the growth of pathogenic bacteria hyphae. A biological control agent prepared from fermentation liquor of the trichoderma viride strain FS50 can remarkably promote increase of the plant height, the chlorophyll content, the root activity, the soluble sugar content and the like of grape plants, and has a good control effect on grape root rot; the method can reduce the probability of infection of root rot in the grape planting period, prolong the grape planting age limit and improve the yield and quality of grapes, and has a wide application prospect in the aspect of prevention and treatment of grape root rot.

The present invention relates to the technical field of fertilizer preparation, and in particular to a traditional Chinese medicine composite microbial fertilizer for inhibiting angelica root rot. The composite microbial fertilizer for inhibiting angelica root rot provided by the present invention comprises 45-55 parts of composite fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of a composite microbial agent, and 8-12 parts of a traditional Chinese medicine extract. The composite microbial agent comprises Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride, and Trichoderma harzianum, with a mass ratio of 14-18:12-16:11-15:8-12:8-12:5-9:5-9, each containing 400-600 million bacteria per gram. The traditional Chinese medicine extract comprises Scutellaria baicalensis, Ephedra sinica, Tagetes erecta, Nerium oleander, Ginkgo biloba, and Sophora flavescens. This fertilizer not only prevents and treats angelica root rot but also significantly improves the yield and quality of angelica, achieving excellent results.

The invention belongs to the technical field of microorganisms, and particularly relates to trichoderma viride with high cellulase yield and application of the trichoderma viride. The bacterial strain is Trichoderma viride LKT2025-06, and the preservation number of the Trichoderma viride LKT2025-06 is CGMCC (China General Microbiological Culture Collection Center) No. 42215. The enzyme activity level of cellulase filter paper produced through industrial fermentation of the strain can reach 750 U / mL or above, high-activity cellulase can be generated, a certain amount of xylanase and mannanase can also be generated, hemicellulose is decomposed while cellulose is degraded, more comprehensive and efficient degradation of biomass is achieved, and the production cost is reduced. The utilization efficiency of biomass resources is improved.

A biocontrol formulation for plant pathogens includes a Trichoderma viride filtrate and an inert substance selected from the group consisting of a viscosity reducer, a surfactant, flour, clay, and combinations thereof. In an embodiment, the filtrate includes conidia of the Trichoderma viride. In an embodiment, the filtrate includes byproducts obtained from the fermentation of Trichoderma viride. In an embodiment, the byproducts include at least one of the organic acids, growth regulators, and amino acids.

This invention discloses the application of Trichoderma composting agents in compost preparation and a method for compost preparation, belonging to the field of biocomposting technology. It provides the application of Trichoderma composting agents in compost preparation, wherein the Trichoderma composting agents include *Trichoderma longibrachiatum* T6, *Trichoderma viride* P6, and *Trichoderma longibrachiatum* Lu3. *Trichoderma longibrachiatum* T6 is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC NO. 13183 and deposit date November 14, 2016. This invention utilizes Trichoderma composting agents and an aerobic composting method to greatly increase the activity of *Trichoderma* fungi in the compost. Regular aeration and constant temperature composting significantly accelerate the composting speed of straw and cow manure.

A biocontrol formulation for plant pathogens includes a Trichoderma viride filtrate and an inert substance selected from the group consisting of a viscosity reducer, a surfactant, flour, clay, and combinations thereof. In an embodiment, the filtrate includes conidia of the Trichoderma viride. In an embodiment, the filtrate includes byproducts obtained from the fermentation of Trichoderma viride. In an embodiment, the byproducts include at least one of the organic acids, growth regulators, and amino acids.

The invention discloses a compound microorganism bactericide and a preparation method thereof, and relates to the technical field of agricultural microorganism application. Comprising the following components: 10 to 15 parts of a core strain, 3 to 5 parts of humic acid, 4 to 6 parts of plant polysaccharide, 8 to 12 parts of a protective agent, 2 to 4 parts of a nutritional ingredient, 1 to 3 parts of adenosine, 2 to 4 parts of nano-scale hydroxyapatite, 1 to 2 parts of graphene quantum dots, 0.8 to 1.5 parts of glutamine and 1 to 3 parts of carboxymethyl-beta-cyclodextrin. The core strains comprise paecilomyces lilacinus, bacillus amyloliquefaciens, trichoderma harzianum, trichoderma viride, bacillus subtilis, pseudomonas fluorescens, bacillus licheniformis, brevibacillus laterosporus and candida utilis. The bactericide is remarkable in sterilizing effect, sufficient in stability and good in field control effect.

This invention relates to the field of bioprotective materials technology and discloses a composite insect-repellent and mildew-proof coating liquid. The core antibacterial and insecticidal components are a blend of clove bud essential oil, garlic essential oil, and Australian tea tree essential oil. A composite surfactant is prepared using castor oil polyoxyethylene ether, transparent hydrogenated castor oil, and ethanol, which is then emulsified to obtain a composite essential oil emulsion. This emulsion is then blended with a shellac coating liquid to obtain the coating liquid. Through the synergistic effects of essential oil blending, emulsion stabilization, and shellac film-forming slow release, this invention achieves long-lasting 100% anti-mildew efficacy against Aspergillus niger, Trichoderma viride, and Penicillium citrinum, as well as 100% contact killing effect on mealworm larvae within 2 days. It also features uniform film formation, strong adhesion, long protection period, low essential oil dosage, mild odor, green and residue-free operation, and simple and easily scalable processing. It can be widely used for long-lasting insect and mildew protection in mycelial materials, food storage, agricultural product preservation, and feed storage.

The invention discloses a carbon-based bio-organic fertilizer with phosphorus immobilization and biological activation functions and application, and belongs to the technical field of agricultural environments and novel fertilizers. The organic fertilizer is prepared by compounding a high-performance phosphorus adsorption carbon-based material, a low-phosphorus leaching-loss organic fertilizer carrier, a composite functional microbial agent and fulvic acid, wherein the carbon-based material is fir sawdust carbon or rice hull carbon with the specific surface area being larger than or equal to 19 m < 2 > / g and the micropore proportion being larger than or equal to 60%, and the organic fertilizer carrier is decomposed sheep manure organic fertilizer; the microbial agent is prepared from trichoderma viride Tv41 and trichoderma longibrachiatum TB2; according to the organic fertilizer, a'carbon-fertilizer-bacterium 'ternary synergistic system is constructed, so that the bio-availability of the organic fertilizer is enhanced while soil phosphorus is effectively immobilized, the problem that immobilization and activation are difficult to cooperate in the traditional technology is solved, phosphorus loss is synchronously controlled and the absorption and utilization efficiency of crops on phosphorus is improved under the condition of reducing the dosage of a phosphate fertilizer, and the yield of the crops is increased. The method is suitable for application of crops such as cabbages and hot peppers in phosphorus-enriched vegetable fields.

This invention discloses a biocontrol compound microbial strain YC and its application, relating to the field of microbial technology. The biocontrol compound microbial strain YC is composed of *Trichoderma harzianum* YP2 and *Trichoderma viride* CK1A. *Trichoderma harzianum* YP2 was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and is classified as follows: Trichoderma afroharzianum YP2, with accession number CGMCC NO. 41947; the described *Trichoderma viride* CK1A was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and classified as... Trichoderma atroviride CK1A, with accession number CGMCC NO. 41948. The biocontrol compound microbial system YC described herein is used to control maize stalk rot. The biocontrol compound microbial system YC of this invention exhibits stable efficacy and strong antibacterial ability, opening up new avenues for the biological control of maize stalk rot in my country.

The invention provides an acid-resistant compound microbial agent for producing cellulase and amylase as well as a preparation method and application of the acid-resistant compound microbial agent, and belongs to the technical field of microbial fermentation. The compound microbial agent disclosed by the invention mainly comprises acid-resistant aspergillus niger capable of producing amylase, acid-resistant trichoderma viride strains capable of producing cellulase, acid-resistant bacillus licheniformis capable of producing amylase and cellulase and acid-resistant saccharomyces cerevisiae, and moldy bran obtained by fermentation of the compound microbial agent has the advantages of acid resistance, high yield, high yield and the like. And amylase and cellulase can be produced, and the method can be applied to fermentation raw materials with relatively low pH.

The invention relates to the technical field of genetic engineering, in particular to an engineering bacterium capable of producing 2-halogenated acid dehalogenase at high yield as well as a construction method and application of the engineering bacterium. Trichoderma viride 2-halogenated acid dehalogenase gene TvHAD1 is over-expressed in escherichia coli or pichia pastoris, engineering bacteria of the 2-halogenated acid dehalogenase are constructed, and the engineering bacteria of the high-yield 2-halogenated acid dehalogenase are obtained through collaborative optimization of fermentation processes such as induction conditions and metal ions. And efficient heterologous expression and large-scale preparation of the enzyme are realized. Under the shake flask scale, the enzyme yield of the recombinant pichia pastoris engineering bacteria can reach 102.51 mg / L, the enzyme yield of the recombinant escherichia coli engineering bacteria is 21.07 mg / L, and high yield of the 2-halogenated acid dehalogenase is realized.