71results about How to "Promote biotransformation" patented technology

The present invention belongs to the field of biological sterol transforming technology, and is especially biological sterol transforming process with cyclodextrin and its derivative and its preparation. In a transforming system, cyclodextrin or its derivative in certain amount is added, or substrate and cyclodextrin or its derivative in certain ratio are made to form inclusion compound, so as to facilitate transformation. The process of the present invention is especially suitable for microbial transformation of hydrophobic compound, and can raise the solubility of reaction substrate in water solution, raise the substrate utilizing rate and reaction rate, release substrate and product inhibition and raise product converting rate. When the substrate concentration is 3-20g/L, the present invention has conversion period shorted to 3-4 days, conversion rate of 50-95 % and high industrial application value.

The invention relates to a preparation method and application of a fermented angelica extract with sweet aromas. The preparation method comprises the following steps: performing low-temperature pulverization on angelica roots, performing pressurized cell wall breaking, adding a compound biological agent for bioconversion of the angelica, performing incubating conversion for some time, performing programmed pressurization and extraction with an ethanol-water solution, and removing precipitate impurities through high-speed low-temperature refrigerated centrifugation to obtain the fermented angelica extract with sweet aromas. In the fermented angelica extract with sweet aromas, large-molecule irritant substances of the angelica after the bioconversion of the compound biological agent are aroma substances or aroma precursors, and the aroma components thereof are purified with the ethanol-water solution through graduated pressurization and circulated extraction and are purified through high-speed low-temperature refrigerated centrifugation, so that large-molecule impurities such as proteins, polysaccharides and the like are reduced, the total aroma amount and the aroma variety of the angelica extract are increased, the quality of the angelica extract is improved, the aromas are more abundant and natural, and natural complex sweet aromas and medicine aromas are combined. When being used in a tobacco shred formula, the fermented angelica extract coordinates with smoke to send out clear tobacco aromas, the aromas are abundant and natural and are natural complex ones combining the sweet aromas and the medicine aromas, and the irritation and the offensive odor are reduced. Thus, the defects of the angelica extract not subjected to bioconversion and extraction, such as being turbid in smoke, being obvious in medicine aromas and being high in irritation, are overcome.

The invention aims to provide a preparation method and applications of an iris extract. The preparation method comprises following steps: slicing and griding an iris root, adding a composite biological preparation to carry out biological conversion and fermentation, freeze-drying the fermentation broth in vacuum to obtain an iris fermentation product, putting the iris fermentation product in a micro-filtration bag, carrying out Soxhlet reflux extraction by a using ethanol solution, freeze-drying the extract in vacuum to obtain the iris extract. The obtained iris extract has few impurities, isrich in aroma types, and has the advantages that the purity of active components such as flavone is high, and the aroma quality is improved. The shortages that the characteristic aroma of an iris extract, which has not been biologically converted and extracted, is prominent and the irritation is fierce are overcome. Moreover, the operation of the technology is simple, the technological conditionsare mild, filtrate can be directly obtained without using time wasting suction filtration, during the technological process, a low temperature is maintained, the aroma generating components and otheractive components of the iris root are well preserved therefore, and the iris extract is applied to conventional cigarettes and novel cigarettes, and can improve the aroma quality, enrich the aroma, increase aged tobacco fragrance, and improve the smoke and taste.

The invention belongs to the technical field of biological prevention and control. Studies find that quercetin, rutin or tea polyphenol can activate GSTs (glutathione S-transferase), UGT (uridine diphosphate glucuronyl transferase), SULT (sulfotransferase) or AANAT (Arylamine N-acetyltransferase) in the non-contaminated prawn intestinal tract, so that the biotransformation of II phase enzyme is promoted; the activity of the entogenous detoxifying enzyme in the contaminated prawn intestinal tract is induced by adding an inductive agent into prawn feed, so that the metabolism of the prawn vivotoxin is accelerated; the toxin residue is reduced or cleared away; the prawn food safety is ensured.

The invention discloses an isoflavone aglycone-enriched soybean protein and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a glucosaccharase solution, preparing a pure natural soybean isoflavone HAC-NaAC dispersion solution, then adding the glucosaccharase solution into the dispersion solution, and performing enzymolysis to obtain soybean isoflavone aglycone; (2) adding deionized water into low-temperature degreased soybean meal, regulating the pH value, sufficiently stirring, performing centrifugal slag removal on the uniformly dispersed soybean meal solution, and dialyzing the supernatant to obtain crude soybean meal slurry; (3) mixing the isoflavone aglycone and the crude soybean meal slurry, regulating the pH value, performing injection cooking, and quickly cooling the obtained solution in an ice bath; and (4) dialyzing the cooled solution, and performing spray drying to obtain the isoflavone aglycone-enriched soybean protein. The total aglycone amount of the soybean protein disclosed by the invention can be 100-600 times more than the aglycone amount of a common soybean protein isolate; and the soybean protein is low in production cost, simple in process and stable in nature.

A preparation method for an angelica sinensis extract with baking fragrance, and an application of the same are provided. Angelica sinensis is smashed at low temperature and cell disruption is obtained via pressurization; composite biological agent is added to convert the smashed angelica sinensis; after certain time for incubation and conversion, the angelica sinensis is heated at high temperature, pressurized and then extracted; a high-temperature pyrolysis and fragrance enhancement reaction is generated; settlement impurities are removed via low-temperature refrigeration centrifugal operation;angelica sinensis extract with baking fragrance can be achieved; macromolecule impurities such as protein and polysaccharide can be removed; through composite biological agent conversion and high-temperature and high-pressure pyrolysis reaction, a large amount of aroma components and fragrance precursor can be produced; fragrance quality of the angelica sinensis extractcan be enhanced; rich andnatural fragrance is provided; baking fragrance and medicine fragrance can be cooperated; when applied to formula tobacco fillers, the angelica sinensis extract can be cooperated with smoke smell, and clear cigarette fragrance can be produced and herbal fragrance, baking fragrance and flowering wood fragrance can be cooperated; rich and natural fragrance is provided; and deficiencies of muddy smoke and obvious medicine fragrance of the angelica sinensis extract failing to be biologically converted and reacted via high-temperature and high-pressure pyrolysis can be overcome.

The invention provides a preparing method and application of volatile limette oil. The preparing method includes the steps that peel and pulp of limette are separated, kernels are removed, the peel and residues obtained after a limette juice is obtained by squeezing the pulp of the limette are mixed, smashed at low temperature and subjected to vacuum pressurization for wall breaking, acid is addedfor hydrolysis, a compound biological agent is added for fermentation incubation, and after biotransformation lasts for a period of time, the volatile limette oil is extracted through steam distillation. The volatile limette oil is applied into traditional-cigarette-formula cut tobacco and novel-cigarette-tobacco atomization cut tobacco and coordinates with smoke, cigarette fragrance is clear, the fragrance is rich and natural, fruit fragrance, orange flower fragrance, round cardamom fragrance, purple perilla fragrance and grease herb cream fragrance are achieved, sweet fragrance is brought to cigarettes and tobacco, the fragrance is permeable, and the defects that when volatile limette oil extracted without biotransformation is applied into cigarettes, characteristic fragrance is remarkable, limette fragrance is prominent, and slight irritation occurs are overcome.

The invention discloses a yield-increasing controlled-release organic fertilizer for rice. The yield-increasing controlled-release organic fertilizer is prepared from the following raw materials of animal manure, biogas residue, cake meal, weathered coal, ground phosphate ore, ground potassium ore, diatomite, attapulgite, borax, sodium polyacrylate, fulvic acid, sodium alginate, xylitol residue, tangerine residue, bamboo fiber, trace element fertilizer, microbial agent, rooting powder, and film coating agent. The yield-increasing controlled-release organic fertilizer for the rice has the advantages that the formula is reasonable, the rich nutrients are contained, the fertilizer demand rule of the rice is met by the releasing of nutrients, the controlled-release effect is good, the fertilizer fertility is high, the pollution to water source and soil by fertilizer applying is effectively decreased, the ecological environment of the rice field is improved, the soil is fertilized, the output of the rice is effectively increased, the quality of the rice is improved, and the sustained development of agricultural in China is promoted.

The invention belongs to the technical field of traditional Chinese medicine and relates to a programmed temperature control sweating and drying processing method of traditional Chinese medicine radixscrophulariae, comprising the following steps: 1) selecting fresh radix scrophulariae collected when stem and leaf wither in winter, removing rhizome, bud, fibrous root and sediment, and heating andbaking the fresh radix scrophulariae in a temperature-programmed baking mode; 2) after heating and baking, taking out that scrophularia root while it is hot, sealing the scrophularia root with a plastic bag for piling up and sweating, or simulating sweating through a temperature control device; 3) low-temperature baking the radix scrophulariae after sweat treatment; 4) after that low-temperature baking is completed, the perspiration is performed again according to the perspiration mode; and 5) baking radix scrophulariae at low temperature until that moisture content is less than 16%, taking out and cooling to obtain the finished product. The invention provides a radix scrophulariae sweating processing method capable of realizing high-quality primary processing of radix scrophulariae and obtaining scrophulariae radix Chinese medicinal materials with excellent internal quality.

The invention discloses recombinant butyric acid bacillus methylotrophicus as well as a construction method and application thereof. By taking bacillus methylotrophicus as a source, the genetically engineered bacteria for over-expression methanol utilization and butyric acid synthesis are constructed, i.e. BM/pXY1-mtaA/mtaB/mtaC2 and BM/pXY1-atoB/paaH/crt/bcd. The methanol utilization capacity and the butyric acid synthesis capacity of the recombinant strain are remarkably enhanced, and the methanol consumption capacity in a PB culture medium is improved by 69% and 14% respectively; and the yield of butyric acid is respectively increased by 38% and 28.6%. A foundation is laid for further improving the consumption capacity of methanol.

The invention discloses a method for performing pretreatment and biotransformation by adding an acidic reagent in a lignocellulose raw material densification process. The method comprises the following steps: adding the acidic reagent and water into a lignocellulose raw material, performing uniform mixing, and performing densification treatment; or pretreating the lignocellulose raw material by anacid method, then performing densification treatment to obtain densified lignocellulose, then directly performing biotransformation, or performing biotransformation after subsequent selective pretreatment to produce a target chemical. The acidic reagent is added in the lignocellulose raw material densification process to destroy the original structure of lignocellulose, so that the mild pretreatment process of the lignocellulose raw material is realized in the subsequent transportation and storage processes, and the severity of further pretreatment is reduced. Compared with a loose raw material, obtained lignocellulose particles are higher in density and convenient to transport and store, the pretreatment cost is reduced, and the yield of a target product is higher in biotransformation ofthe acidified and densified lignocellulose.

This invention relates to a method for preparing enzymolysis aid of cellulose from crop straws. The method comprises: (1) washing crop straws with water, and filtering to obtain cell wall filter residue; (2) adding 0.1-0.5 mol/L NaOH solution containing 20-50 mM ascorbic acid Vc, 0.1-0.5 wt.% mercaptoethanol, 1-5 mM PMSF and 5-10 mM EDTA; (3) keeping at 30-70 deg.C for 10-30 h, adding PMSF every 3-5 h until the final concentration is 1-5 mM, and extracting; (4) centrifuging after extraction, and collecting the supernatant; (5) adjusting the pH value of the supernatant with HCl, adding ethanol, shaking uniformly, standing, centrifuging, collecting the precipitate, and vacuum-drying at low temperature to obtain enzymolysis aid of cellulose 10 wt.% of cell walls of crop straws. The method has such advantages as abundant raw materials, and low cost.

The invention discloses a Chinese herbal medicinal micro-ecological premixed feed. The Chinese herbal medicinal micro-ecological premixed feed comprises the Chinese herbal medicine raw materials and the microbial agents. The Chinese herbal medicine raw materials comprise honeysuckle flower, dandelion, radix astragali, bighead atractylodes rhizome, poria cocos, hyacinth bean, Chinese yam, hawthorn, cortex acanthopanacis, rhizoma atractylodis, oriental waterplantain rhizome, prepared fleece flower root, and gynostemma pentaphylla; and the microbial agents comprise streptococcus faecalis, bacillus subtilis, bacillus coagulans, pediococcus acidilactici, lactobacillus plantarum, microzyme, ferrous glycine chelate, and tryptophan. The Chinese herbal medicine raw materials and the microbial agents are mixed according to the proportion of 45-55% of the Chinese herbal medicine raw materials and 45-55% of the microbial agents. The invention also provides the preparation method of the Chinese herbal medicinal micro-ecological premixed feed. During the preparation processes of the Chinese herbal medicinal micro-ecological premixed feed, physiologically active substances having special effect for increasing livestock and poultry immunity are generated, sub-health level of the livestock and poultry in the large-scale culture fields can be improved, and the resistance capability of the livestock and poultry on invasion by harmful microbes can be increased.

The invention discloses a bacillus subtilis feed additive. The bacillus subtilis feed additive is characterized by being prepared through the steps: uniformly mixing bacillus subtilis, fructus hippophae freeze-dried powder, roxburgh rose freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder and cactus freeze-dried powder, then adding residual fermented eggshell powder and uniformly mixing, and carrying out compound packaging; in every 1000g of the bacillus subtilis feed additive, the quantity of the bacillus subtilis is 1000 billion to 12000 billion, the content of the fructus hippophae freeze-dried powder is 5g to 50g, the content of the roxburgh rose freeze-dried powder is 5g to 50g, the content of the wintercherry freeze-dried powder is 3g to 30g, the content of the blackcurrant freeze-dried powder is 3g to 30g, the content of the cactus freeze-dried powder is 50g to 150g, and the balance is the fermented eggshell powder. The bacillus subtilis feedadditive disclosed by the invention is a single type bacillus subtilis feed additive, is clear in variable bacteria quantity, and is beneficial for clear application and quantitative selection of users; the fructus hippophae freeze-dried powder, the roxburgh rose freeze-dried powder, the wintercherry freeze-dried powder, the blackcurrant freeze-dried powder, the cactus freeze-dried powder and thefermented eggshell powder are utilized as matrixes of the bacillus subtilis feed additive, so that the nutritive value of the bacillus subtilis feed additive is increased, meanwhile, the metabolism ofanimals is promoted, the disease resistance of a body is increased, growth of the animals is promoted, and the economic benefit of breeding industry is finally increased.

The invention relates to the field of a fertilizer, and relates to a selenium-rich foliar fertilizer. The selenium-rich foliar fertilizer comprises the following raw materials in parts by weight: 5-10 parts of sodium selenate, 5-8 parts of vitamin, 1-3 parts of coenzyme II, 5-10 parts of amino acid, 2-5 parts of potassium chloride, 2-5 parts of yeast liquid, 0.08-0.12 parts of EDTA-Mg, 0.08-0.12 parts of EDTA-Fe, 0.06-0.1 parts of boric acid, 0.04-0.06 parts of EDTA-Mn, 0.04-0.06 parts of EDTA-Cu, 0.04-0.06 parts of zinc sulfate, and 0.004-0.01 parts of sodium molybdate. selenium element and various trace elements are rapidly supplemented through leaf surface, the waste of the substances can be effectively avoided, absorptivity is ensured, and the soil pollution is reduced.

The invention discloses a preparation method of a ginkgo leaf feed additive. The method comprises the following steps of weighing ginkgo leaves, mulberry leaves and broussonetia papyrifera leaves according to a weight ratio of 1: 1: 10, carrying out microwave drying, crushing and uniform mixing, adding red yeast rice powder accounting for 5% of the total weight and lactic acid bacteria freeze-dried powder accounting for 3% of the total weight, adding beer accounting for 60% of the total weight of the medicinal materials, carrying out uniform mixing, and performing fermentation at 30-32 DEG C for 16-18 hours; and performing fermentation at 26-28 DEG C for 60-70 days, and after the fermentation is finished, performing blow-drying with cold air in a shade place until the water content is lessthan or equal to 30% (by weight), thereby obtaining the ginkgo leaf feed additive product. The invention also provides a new method for development and application of the ginkgo leaves, the mulberryleaves and the broussonetia papyrifera leaves. The method has relatively good social effects for the development and utilization of enriched nutrients of the plant leaves as the feed additive, the efficient breeding and rapid growth of bred animals, the promotion of the development of the breeding industry and the improvement of the economic benefits.

The invention discloses lycium ruthenicum sauce. The lycium ruthenicum sauce is produced by the method comprising the following steps: fermenting lycium ruthenicum juice to obtain fermented lycium ruthenicum juice; concentrating to obtain lycium ruthenicum concentrate; and mixing with the existing sauce in ratio of 1: 20. The production method is simple, easy to carry out, short in production period, low in energy consumption and high in efficiency; the prepared sauce has no additives like hydrolyzed vegetable protein seasoning liquid, monosodium glutamate and spice, remains the taste of lyceum, is fresh, delicious and pure, and nutrient-rich, and is a beneficial and healthy seasoning.

The invention discloses a catechin biological conversion nutrient solution and a method for promoting catechin biological conversion. The catechin biological conversion nutrient solution is prepared from raw materials containing catechin, a nitrogen source compound and water; the raw materials containing catechin are tea polyphenol or catechin; the mass percentage of the nitrogen source compound is 0.01-5%. The catechin biological conversion nutrient solution is simple in ingredient, contains no other complex inorganic salt, and causes no interference with catechin EGCG and the like; the catechin biological conversion nutrient solution is simple, convenient to use, rapid, environmentally friendly, low in cost and capable of being widely applied to the field of food and medicine; the addednitrogen source compound can improve the tannase activity, catechin EGCG is rapidly converted, and biological conversion of other catechin ECG, GCG, EGC, EC, GA, GC, CG, C and the like through aspergillus can be promoted.

The invention discloses a bacillus natto feed additive. The bacillus natto feed additive is characterized by being prepared through the steps: uniformly mixing bacillus natto, fructus hippophae freeze-dried powder, roxburgh rose freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder and cactus freeze-dried powder, then adding residual fermented eggshell powder anduniformly mixing, and carrying out compound packaging; in every 1000g of the bacillus natto feed additive, the quantity of the bacillus natto is 1000 billion to 12000 billion, the content of the fructus hippophae freeze-dried powder is 5g to 50g, the content of the roxburgh rose freeze-dried powder is 5g to 50g, the content of the wintercherry freeze-dried powder is 3g to 30g, the content of the blackcurrant freeze-dried powder is 3g to 30g, the content of the cactus freeze-dried powder is 50g to 150g, and the balance is the fermented eggshell powder. The bacillus natto feed additive disclosedby the invention is a single type bacillus natto feed additive, is clear in variable bacteria quantity, and is beneficial for clear application and quantitative selection of users; the fructus hippophae freeze-dried powder, the roxburgh rose freeze-dried powder, the wintercherry freeze-dried powder, the blackcurrant freeze-dried powder, the cactus freeze-dried powder and the fermented eggshell powder are utilized as matrixes of the bacillus natto feed additive, so that the nutritive value of the bacillus natto feed additive is increased, meanwhile, the metabolism of animals is promoted, the disease resistance of a body is increased, growth of the animals is promoted, and the economic benefit of breeding industry is finally increased.