Isoflavone aglycone-enriched soybean protein and preparation method thereof

A technology of isoflavone aglycone and soybean protein, which is applied in the direction of plant protein processing, etc., can solve the problems of low content of soybean isoflavones and low bioavailability, and achieve the effect of improving the ability of hydrophobic binding and weakening the electrostatic interaction.

Active Publication Date: 2013-08-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the deficiencies of the above-mentioned prior art, the object of the present invention is to provide a method for preparing soybean protein rich in isoflavone aglycone, which overcomes the low content of soybean isoflavones and the Natural soybean isoflavones mainly exist in the form of glycosides and have the defects of low bioavailability

Method used

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  • Isoflavone aglycone-enriched soybean protein and preparation method thereof
  • Isoflavone aglycone-enriched soybean protein and preparation method thereof
  • Isoflavone aglycone-enriched soybean protein and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] A preparation method of soybean protein rich in isoflavone aglycone, comprising the steps of:

[0051] Step 1: Prepare glucosidase enzyme solution (1200U / g), disperse 6.15g of pure natural soybean isoflavones in 370mL HAC-NaAC buffer solution, then add 30mL of enzyme solution to 370mL of dispersion solution, at 65°C and pH6 Stirring and enzymatic hydrolysis for 2.5h under the condition of 0.0 to obtain soybean isoflavone aglycone;

[0052] Step 2: Pass 1000g of low-temperature defatted soybean meal powder (NSI=89, protein content 51%) through a 60-mesh sieve, add 10L of deionized water at a material-liquid ratio of 1:10w / v, adjust the pH to 7.5 and stir for 1.5h, then centrifuge at 10000g After 30 minutes, the supernatant was fully dialyzed to obtain a coarse soybean meal slurry;

[0053] Step 3: Mix and stir the soybean meal slurry dialyzed in Step 2 with isoflavone aglycone for 60 minutes, adjust the pH to 8.0, place it in a jet cooker for 30 seconds at 100° C., and ...

Embodiment 2

[0071] A preparation method of soybean protein rich in isoflavone aglycone, comprising the steps of:

[0072] Step 1: Prepare glucosidase enzyme solution (1200U / g), disperse 6.15g of pure natural soybean isoflavones in 370mL HAC-NaAC buffer solution, then add 30mL of enzyme solution to 370mL of dispersion solution, at 65°C and pH6. Stirring and enzymatic hydrolysis for 2.5 hours under 0 conditions, to obtain soybean isoflavone aglycone;

[0073] Step 2: Pass 1000g of low-temperature defatted soybean meal powder (NSI=89, protein content 51%) through a 60-mesh sieve, add 10L of deionized water at a material-liquid ratio of 1:10w / v, adjust the pH to 7.5 and stir for 1.5h, then centrifuge at 10000g After 30 minutes, the supernatant was fully dialyzed to obtain a coarse soybean meal slurry;

[0074] Step 3: mixing the dialyzed soybean meal slurry and isoflavone aglycone in step 2 for 60 minutes, adjusting the pH to 6.0, placing it in a jet cooker for 140°C and 90s jet cooking, and...

Embodiment 3

[0081] A preparation method of soybean protein rich in isoflavone aglycone, comprising the steps of:

[0082] Step 1: Prepare glucosidase enzyme solution (1200U / g), disperse 6.15g of pure natural soybean isoflavones in 370mL HAC-NaAC buffer solution, then add 30mL of enzyme solution to 370mL of dispersion solution, at 65°C and pH6. Stirring and enzymatic hydrolysis for 2.5 hours under 0 conditions, to obtain soybean isoflavone aglycone;

[0083] Step 2: Pass 1000g of low-temperature defatted soybean meal powder (NSI=89, protein content 51%) through a 60-mesh sieve, add 10L of deionized water at a material-liquid ratio of 1:10w / v, adjust the pH to 7.5 and stir for 1.5h, then centrifuge at 10000g After 30 minutes, the supernatant was fully dialyzed to obtain a coarse soybean meal slurry;

[0084] Step 3: Mix and stir the dialyzed soybean meal slurry and isoflavone aglycone for 60 minutes, adjust the pH to 6.0, place in a jet cooker for 140° C., 90 s jet cooking, and rapidly coo...

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Abstract

The invention discloses an isoflavone aglycone-enriched soybean protein and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a glucosaccharase solution, preparing a pure natural soybean isoflavone HAC-NaAC dispersion solution, then adding the glucosaccharase solution into the dispersion solution, and performing enzymolysis to obtain soybean isoflavone aglycone; (2) adding deionized water into low-temperature degreased soybean meal, regulating the pH value, sufficiently stirring, performing centrifugal slag removal on the uniformly dispersed soybean meal solution, and dialyzing the supernatant to obtain crude soybean meal slurry; (3) mixing the isoflavone aglycone and the crude soybean meal slurry, regulating the pH value, performing injection cooking, and quickly cooling the obtained solution in an ice bath; and (4) dialyzing the cooled solution, and performing spray drying to obtain the isoflavone aglycone-enriched soybean protein. The total aglycone amount of the soybean protein disclosed by the invention can be 100-600 times more than the aglycone amount of a common soybean protein isolate; and the soybean protein is low in production cost, simple in process and stable in nature.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to the preparation of soybean protein rich in soybean isoflavone aglycone with health care significance, which can be used in special health food for menopausal women and blood fat-lowering or medical clinical nutrition products. Background technique [0002] Soy isoflavones are a class of phytoestrogens that exist in two forms: aglycones and glycosides. Soybean isoflavone glycosides are converted into biologically active isoflavone aglycones under the action of intestinal bacteria and microbial β-glucosidase enzymes. This type of active molecule can be absorbed in the body after 5 to 10 times of repeated intestinal and hepatic circulation. It can be absorbed and utilized and can play an important estrogen-like role. For various reasons, the human body's ability to convert isoflavone aglycones varies greatly. [0003] Studies have found that soy protein containing soy isofla...

Claims

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Application Information

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IPC IPC(8): A23J3/16
Inventor 杨晓泉杨娟王金梅黄旭
Owner SOUTH CHINA UNIV OF TECH
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