35results about How to "Weaken electrostatic interactions" patented technology

A liquid jet is modulated to selectively cause the jet to break off into drop pairs and third drops traveling along a path using a drop formation device associated with the jet. Each drop pair is separated on average by a drop pair period and includes a first and second drop in response to input image data. The third drops, separated on average by the same drop pair period, are larger than the first and second drops in response to input image data. A waveform provided by a charging device has a period that is equal to the drop pair period, includes first and second distinct voltage states, and is independent of input image data. The charging device, synchronized with the drop formation device, produces first and second charge states on the first and second drops, respectively, of the drop pairs and a third charge state on the third drops.

A group timing delay device shifts the timing of drop formation waveforms supplied to drop formation devices of one of first and second nozzle groups so that print drops from the nozzle groups are not aligned relative to each other along a nozzle array direction. A charging device includes a common charge electrode associated with liquid jets from the nozzle groups and a source of varying electrical potential between the charge electrode and liquid jets which provides a charging waveform that is independent of a print and non-print drop pattern. The charging device is synchronized with the drop formation devices and the group timing delay device to produce a print drop charge state on print drops of a drop pair, a first non-print drop charge state on non-print drops of the drop pair, and a second non-print drop charge state on third drops.

A method for purifying a protein using Protein A chromatography comprising a) absorbing the protein to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed protein with a buffer comprising one or more chaotropic agents in combination with one or more hydrophobic modifiers and having a pH of at least 7.0; and c) eluting the protein from the Protein A immobilized on the solid support.

The present invention provides a highly electro-conductive electrophotographic member which contributes to formation of high-quality electrophotographic images while bleeding out of an ion conducting agent is reduced, a process cartridge, and an electrophotographic apparatus. Accordingly, the electrophotographic member according to the present invention includes an electro-conductive mandrel and an electro-conductive layer, wherein the electro-conductive layer contains a resin synthesized from a nitrogen-containing aromatic heterocyclic cation and a compound being able to react with the nitrogen-containing aromatic heterocyclic cation, and an anion; the nitrogen-containing aromatic heterocyclic cation has two substituents bonded to hydroxyl groups; and the substituent bonded to the hydroxyl group is bonded to a nitrogen atom of a nitrogen-containing aromatic heterocycle of the nitrogen-containing aromatic heterocyclic cation.

The invention discloses a lipopolysaccharide amine cationic polymer, which is synthesized with hydrophilic polyaldehyde-based sodium alginate is adopted as a main chain, hydrophobic cholesterol capped polyethylene imine of low molecular weight as a side chain. The physical and chemical structure, buffering capacity, transfection efficiency and cytotoxicity of the lipopolysaccharide amine cationic polymer are characterized. According to exploration of the feasibility of use as a gene carrier and the effects on improvement of the gene transfection efficiency and reduction of the cytotoxicity, the polymer has advantages of good biocompatibility, controllable degradation performance, low cytotoxicity, high safety and practicality and low cost, and the transfection efficiency of GFP (green fluorescent protein) in MSCs (mesenchymal stem cells) is higher than 95 percent regardless of the presence or absence of serum. Moreover, the polymer can be used as a gene carrier and other drug carriers such as anticancer drugs, RNA (ribose nucleic acid) carriers and the like. The invention also discloses a preparation method and application of the polymer.

Drop formation devices are provided with drop formation waveforms to modulate liquid jets to cause portions of the liquid jets to form print drops having a jet breakoff length L_p in a print drop breakoff length range R_p and non-print drops having a jet breakoff length L_np in a non-print drop breakoff length range R_np. A timing delay device shifts the timing of the waveforms supplied to drop formation devices of first and second nozzle groups so that print drops formed from first and second nozzle groups are not aligned relative to each other. A charging device includes a charge electrode that is positioned relative to the breakoff length L_p and breakoff length L_np such that there is a difference in electric field strength at the two breakoff lengths to produce a print drop charge state on print drops and a non-print drop charge state on non-print drops.

Provided is an organic nanotube having a hydrophobized inner surface, formed by molecules including an asymmetric bipolar lipid molecule represented by the following General Formula (1) and a derivative thereof represented by the following General Formula (2), wherein the organic nanotube has a hydrophilized outer surface and a hydrophobized inner surface of a hollow cylinder and is formed by binary self-assembly, the organic nanotube encapsulates a hydrophobic guest in the hollow cylinder, has a function of refolding a denatured protein, and has a function of sustainably-releasing a hydrophobic drug according to the change in hydrophobicity of the inner surface of the tube or external stimulus,

In Formulas (1) and (2), wherein the same symbols have the same meanings, G is a 1-glucopyranosyl group or 2-glucopyranosyl group, and n is an integer of 12 to 22.

Particularly, an asymmetric bipolar lipid molecule and an ester thereof respectively represented by general formulae (1) and (2) wherein n is an integer of 18 to 22, both of Z₁ and Z₂ are single bonds, Y is Gly, m(s) is the same or different integer of 3 to 6, X is OH, and R is a methoxy group, an ethoxy group, or a benzyloxy group are novel substances, and can form a carboxylic acid based asymmetric nanotube by single component self-assembly or binary self-assembly.

The invention discloses an isoflavone aglycone-enriched soybean protein and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a glucosaccharase solution, preparing a pure natural soybean isoflavone HAC-NaAC dispersion solution, then adding the glucosaccharase solution into the dispersion solution, and performing enzymolysis to obtain soybean isoflavone aglycone; (2) adding deionized water into low-temperature degreased soybean meal, regulating the pH value, sufficiently stirring, performing centrifugal slag removal on the uniformly dispersed soybean meal solution, and dialyzing the supernatant to obtain crude soybean meal slurry; (3) mixing the isoflavone aglycone and the crude soybean meal slurry, regulating the pH value, performing injection cooking, and quickly cooling the obtained solution in an ice bath; and (4) dialyzing the cooled solution, and performing spray drying to obtain the isoflavone aglycone-enriched soybean protein. The total aglycone amount of the soybean protein disclosed by the invention can be 100-600 times more than the aglycone amount of a common soybean protein isolate; and the soybean protein is low in production cost, simple in process and stable in nature.

A group timing delay device is provided to shift the timing of drop formation waveforms supplied to drop formation devices of nozzles of one of first and second groups so that print drops formed from nozzles of the first and second groups are not aligned relative to each other along a nozzle array direction. A charging device includes a common charge electrode associated with liquid jets formed from the nozzles of the first and second group and a source of varying electrical potential between the charge electrode and liquid jets. The source of varying electrical potential provides a charging waveform that is independent of print and non-print drop patterns. The charging device is synchronized with the drop formation device and the group timing delay device to produce a print drop charge state on print drops and a non-print drop charge state on non-print drops.

Drop formation devices are provided with a sequence of drop formation waveforms to modulate the liquid jets to selectively cause portions of the liquid jets to break off into print drops having a print drop volume V_p and non-print drops having a non-print drop volume V_np. The print and non-print drop volumes are distinct from each other. A timing delay device shifts the timing of drop formation waveforms supplied to drop formation devices of first and second nozzle groups so that print drops from the first and second nozzle groups are not aligned relative to each other. A charging device includes a charge electrode that is positioned in the vicinity of break off of liquid jets to produce a print drop charge state on drops of volume V_p and to produce a non-print drop charge state on drops of volume V_np.

Systems and methods for controlling molecular translocation in solid-state nanopores by edge field leakage. The system dramatically reduces (by orders of magnitude) and controls the fast electrophoretic velocity of molecules to realize sensitive and selective solid-state nanopore sensors for polynucleotides and sequencing platforms.

The invention relates to a thio-isophorone bridge modified double-donor organic optical nonlinear chromophore as well as a synthesis method and application thereof, and six nonlinear optical chromophores Z1-Z6 are designed and synthesized based on a bis (N, N-diethyl) aniline derivative and a thio-isophorone bridge. The invention designs and synthesizes a theoretically driven EO chromophore Z1-Z6 based on a bis (N, N-diethyl) aniline derivative and a thio-isophorone bridge. Particularly, different functional groups such as tert-butyl trimethylsilane, a tert-butyl (methyl) diphenyl silane derivative, 1, 3-bis (trifluoromethyl) benzene and alkylaniline cyanoacetate are used for modifying the bridging part of the chromophore Z2-Z6. And the donor with stronger electron donor capability can generate larger first-grade hyperpolarizability. In addition to relatively high first-order hyperpolarizability, the special structure of the double donors and an isolating group on a chromophore bridge have relatively high spatial effect, so that the material has relatively high polarization efficiency.

The invention discloses a method to modify proteid chip surface, its character is fixing a layer of low molecular weight proteid molecule on the chip by normal method at first, then fixing the proteid chip which is needed to be fixed. The low molecular weight is 1X10 to the power 3 -9X10 to the power 5 Dalton. The method can modify the surface character of the proteid chip surface: on one hand, because of the covering of the low molecular weight molecule on the chip surface, the biology activity of the unfixed proteid molecule can be ensured, and at the same time, the aspecfic absorb is controlled on the surface; on the other hand, the low molecular weight molecule has amino, carboxyl, hydroxyl and other active group polar aminophenol on the proteid molecule surface, they can be used to fix porteid molecule directly after being activeted, and to form stable covalent bond.

The invention relates to a nitrogen and phosphorus co-doped vanadium oxide/carbon self-supporting electrode material and a preparation method and application thereof.The method comprises the steps that at a certain temperature, a vanadium source compound and a carbon source compound are dissolved into an organic solution to obtain a uniform solution, the temperature is kept, then a nitrogen source compound and a phosphorus source compound are added into the solution at certain intervals, and the nitrogen and phosphorus co-doped vanadium oxide/carbon self-supporting electrode material is obtained. A uniform spinning solution is obtained; carrying out electrostatic spinning to obtain a nanofiber film, and then drying to remove the organic solution to obtain a self-supporting electrode precursor; and pre-oxidizing the self-supporting electrode precursor, and then calcining at high temperature for a certain time to obtain the nitrogen and phosphorus co-doped VO/C self-supporting electrode material with a proper amount of oxygen vacancies. The electrode material has a relatively high specific surface area and a proper amount of oxygen vacancy defects and nitrogen and phosphorus heteroatoms, and shows long service life, high specific capacity and excellent cycling stability and rate capability.

The present invention relates to a PARP inhibitor pellet composition and a preparation process therefor. The pellet composition comprises a pellet and an optional additional excipient, with the pellet comprising (1) a pellet core; (2) a drug-containing layer and (3) an optional protective layer, wherein the drug-containing layer contains (a) an active ingredient and (b) a binder; when the composition comprises the protective layer, the protective layer contains (c) a coating material; and the active ingredient is (R)-2-fluoro-10a-methyl-7,8,9,10,10a,11-hexahydro-5,6,7a,11-tetraazacyclohepta[def]cyclopenta[a]fluoren-4(5H)-one, a pharmaceutically acceptable salt thereof and a hydrate thereof.

The invention relates to a method for eluting polypeptide adsorbed to the surface of a meso-porous silicon material. The method is characterized by comprising the step of eluting polypeptide adsorbed to the surface of the meso-porous silicon material by virtue of a mixed solution of organic solvents and a saline solution, wherein the pH value of the saline solution is more than 7 and is not less than two pH units of an isoelectric point of polypeptide with strongest alkalinity. The method is simple and reliable, and the problem that the recovery rate is low when polypeptide is extracted from the meso-porous silicon material is effectively solved.