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Method for performing pretreatment and biotransformation by adding acidic reagent in lignocellulose raw material densification process

A technology for lignocellulose and densification treatment, applied in the field of biotransformation, can solve the problems of high transportation cost and large energy consumption for pretreatment, and achieve the effects of uniform specifications, reduced pretreatment cost, and convenient later operation.

Active Publication Date: 2020-12-22
NANJING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the existing problems of high transportation cost of lignocellulosic raw materials and high energy consumption of subsequent pretreatment, the present invention provides a method for pretreatment by adding acidic reagents in the densification process of lignocellulosic raw materials. Pretreatment of lignocellulose by adding acidic reagents during the chemical process

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  • Method for performing pretreatment and biotransformation by adding acidic reagent in lignocellulose raw material densification process
  • Method for performing pretreatment and biotransformation by adding acidic reagent in lignocellulose raw material densification process
  • Method for performing pretreatment and biotransformation by adding acidic reagent in lignocellulose raw material densification process

Examples

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Comparison scheme
Effect test

Embodiment 1

[0039] This example illustrates the densification process of corn stalks with acid, the steps are as follows:

[0040] (1) Raw material preparation: After collecting the corn stalks in the farmland, dry them naturally and crush them into 1-4mm particles.

[0041] (2) DLA pretreatment: Spray the crushed corn stalks with dilute sulfuric acid solution. The content is 0.5g / g straw dry weight, and the corn straw evenly mixed with the acid reagent is used for densification operation. After the densification is completed, the straws with three acid concentrations are bagged and sealed respectively, and placed at room temperature for more than 1 day.

Embodiment 2

[0043] This example compares the pretreatment effects of DLA, PCS+DA, and LCS+DA hydrothermal pretreatment. Contains the following steps:

[0044] (1) DLA hydrothermal pretreatment process: the raw materials are selected from Example 1, the concentration of sulfuric acid is 0.1g / g dry weight of straw, and pure water is added to make the dry weight of straw: the total water content (including the moisture of the densified straw itself) is 1:9 ; Place it in a high-temperature and high-pressure reactor for heating, the heating temperature is 160° C., and the heating time is 10 minutes.

[0045] (2) PCS+DA hydrothermal pretreatment process: the raw material is no acid-densified straw, the amount of sulfuric acid added to it is 0.1g / g dry weight of straw, and pure water is added to make the dry weight of straw: total water (including dense The moisture content of the straw itself) is 1:9; it is placed in a high-temperature and high-pressure reaction kettle for heating, and the hea...

Embodiment 3

[0050] This example compares the hydrothermal pretreatment effect of DLA with different acid additions. Contains two steps:

[0051] (1) Pretreatment process: the raw materials are selected from Example 1, the concentration of sulfuric acid is 0.1g / g dry weight of straw, 0.05g / g dry weight of straw, and 0.025g / g dry weight of straw, and pure water is added to make the dry weight of straw : The total amount of water (including the moisture of the straw itself) is 1:5; it is placed in a high-temperature and high-pressure reactor for heating, the heating temperature is 120° C., and the heating time is 60 minutes.

[0052] (2) Enzyme hydrolysis process: the hydrolysis substrate concentration is 10% (based on the total mass), adding hydrolase and citric acid-sodium citrate buffer, and placing in a shaking incubator for cultivation (the setting speed is 250rpm, and the temperature is 50°C ) hydrolysis time is 72 hours.

[0053] according to figure 2 shown (Note: figure 2 Among...

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Abstract

The invention discloses a method for performing pretreatment and biotransformation by adding an acidic reagent in a lignocellulose raw material densification process. The method comprises the following steps: adding the acidic reagent and water into a lignocellulose raw material, performing uniform mixing, and performing densification treatment; or pretreating the lignocellulose raw material by anacid method, then performing densification treatment to obtain densified lignocellulose, then directly performing biotransformation, or performing biotransformation after subsequent selective pretreatment to produce a target chemical. The acidic reagent is added in the lignocellulose raw material densification process to destroy the original structure of lignocellulose, so that the mild pretreatment process of the lignocellulose raw material is realized in the subsequent transportation and storage processes, and the severity of further pretreatment is reduced. Compared with a loose raw material, obtained lignocellulose particles are higher in density and convenient to transport and store, the pretreatment cost is reduced, and the yield of a target product is higher in biotransformation ofthe acidified and densified lignocellulose.

Description

technical field [0001] The invention belongs to the technical field of biorefining, and relates to a method for pretreatment and biotransformation by adding an acidic reagent during the densification process of lignocellulose raw materials. Background technique [0002] The energy crisis and environmental pollution have caused the biomass fuel ethanol industry to heat up rapidly. The first generation of biofuel ethanol uses grain as raw material, which will affect food security and feed safety in the long run. This production method has been widely questioned in recent years. The development of non-grain raw material lignocellulosic fuel ethanol production technology has become an inevitable trend of economic and social development (Farrell AE, et al. Ethanol can contribute to energy and environmental goals. Science 2006; 311:506–508.). [0003] Crop straw is an important agricultural by-product and an important cellulose raw material. It is not only abundant in reserves but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10
CPCC12P19/14C12P19/02C12P7/10C12P2201/00C12P2203/00Y02E50/10
Inventor 金明杰袁鑫川陈相雪
Owner NANJING UNIV OF SCI & TECH
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