A kind of method for measuring paraquat blood concentration

Abstract

The invention discloses a method for measuring blood concentration of paraquat. The method comprises the following steps of: (1) pretreating a sample, namely adding aqueous solution of an internal standard substance and a protein precipitation agent acetonitrile into a plasma sample, performing whirl mixing, centrifuging and sampling supernatant; (2) separating the sample, namely adopting a universal C18 liquid chromatographic column, and using an ion pairing agent in an acid mobile phase, wherein the acid mobile phase is mixed solution of 3mmol.L<-1> aqueous solution of sodium dodecyl sulfate, 0.2 percent of aqueous solution of trifluoroacetic acid, acetonitrile and water; and (3) detecting by using a diode array detector, namely detecting by using the diode array detector at a detectionwavelength of 250 to 260nm, measuring peak areas of the internal standard substance and the paraquat, and calculating the blood concentration of the paraquat through least square method linear regression. The plasma sample is easy and convenient to pretreat, the detection process is sensitive and rapid, toxic substances and the blood concentration thereof can be rapidly determined in actual application, and the method is high in clinical application value.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a method for measuring the blood concentration of paraquat. Background technique [0002] Paraquat (paraquat), also known as Kewuzong and Paraquat, has a chemical name of 1,1′-dimethyl-4,4′-bipyridyl dichloride, a molecular weight of 257.2, and is a moderately toxic organic heterocyclic compound. Defoliants and herbicides. Paraquat can be absorbed through the skin, respiratory tract and digestive tract, and distributed in almost all tissues and organs through the blood circulation, with a relatively high concentration in the lungs. The poisoning mechanism is related to the generation of superoxide anion. Since there is no specific antidote at present, the mortality rate after paraquat poisoning is relatively high. Because the blood concentration of paraquat is closely related to mortality, it is necessary to establish a method for determining the blood concentration of...

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Problems solved by technology

For example, "The Determination of Paraquat in Biological Fluids by High Performance Liquid Chromatography" disclosed on pages 388-389, Vol. - The "High Performance Liquid Chromatography for the Determination of Paraquat in Human Blood" published on page 161 uses ion-exchange solid-phase extraction to pretreat paraquat plasma samples, which has the disadvantages of complicated and time-consuming sample processing; In addition, although the ion-pairing reagent sodium octane sulfonate was added to the mobile phase to improve the peak shape of paraquat, the smearing image of paraquat chromatographic peaks still exists; using the external standard method, operating errors will affect the detection results The accuracy; the retention time of paraquat is about 12min, more than 10min

"In vivo distribution of paraquat in rats with acute poisoning by HPLC method" published in "China Law Journal" 2009, Volume 24, Issue 4, pages 267-268, although the internal standard method was used to determine the paraquat in rats, The internal standard is ethyl paraquat, but the pretreatment of the samples still uses the ion-exchange solid-phase extraction method. peak shape, but the trailing image of the paraquat chromatographic peak still exists; the retention time of paraquat is also more than 10min, which is about 15min

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