118 results about "Toxication" patented technology

The invention belongs to the technical field of bioengineering, and particularly relates to a zearalenone toxin degrading enzyme variant and application thereof. The amino acid sequence of the zearalenone toxin degrading enzyme is as shown in SEQ ID NO.2, or the zearalenone toxin degrading enzyme with the amino acid sequence as shown in SEQ ID NO.2 is obtained through amino acid mutation. The zearalenone toxin degrading enzyme mutant is obtained through mutation screening, and compared with a wild type, the zearalenone toxin degrading enzyme mutant has the advantages that the zearalenone degrading capability is obviously improved; besides, the zearalenone toxin degrading enzyme is expressed by using alfalfa, the zearalenone toxin degrading enzyme with biological activity is easy to obtain, and the zearalenone toxin degrading enzyme has certain application potential in prevention and treatment of animal poisoning caused by zearalenone toxin pollution in agriculture and animal husbandry production.

The invention provides a preparation method of (S)-oxetane-2-methylamine. The preparation method of (S)-oxetane-2-methylamine comprises the following steps: (1) carrying out ring closing reaction on a compound A under an alkaline condition to obtain a compound B; (2) reacting the compound B with dibenzylamine to obtain a compound C; (3) reacting the compound C with a reducing agent to obtain a compound D; (4) reacting the compound D with paratoluensulfonyl chloride to obtain a compound E; (5) reacting the compound E with alkali to obtain a compound F; and (6) carrying out a reaction on the compound F and palladium on carbon to obtain the (S)-oxetane-2-methylamine. According to the preparation method provided by the invention, ring expansion reaction using trimethylsulfoxide iodide in the prior art is avoided, the problem that residual sulfur elements cause poisoning of a palladium-carbon catalyst is solved, meanwhile, sodium azide is also avoided, and the preparation method is safe and reliable in process, high in chiral purity and yield and suitable for industrial large-scale production.

The invention discloses a method for determining a quaternary ammonium salt pesticide in blood by using liquid chromatography-mass spectrometry, which comprises the following steps of: drawing a whole blood (plasma and serum) matrix labeling standard working curve through liquid chromatography-mass spectrometry analysis; obtaining peak areas of quantitative ion peaks of target objects containing paraquat, aquacide, diguat, chlormequat and mepiperidine with different concentrations and peak areas of quantitative ion peaks of internal standard substances, and further drawing a standard working curve; and performing liquid chromatography-mass spectrometry analysis on the whole blood sample under the same condition to obtain the peak area of a target substance quantitative ion peak and the peak area of an internal standard substance quantitative ion peak in the whole blood test solution, and calculating the content of each quaternary ammonium salt pesticide according to the standard working curve. The method can be used for simultaneously detecting the content of five quaternary ammonium salt pesticides in the whole blood of the poisoned patient simply, conveniently and quickly, and has the characteristics of simplicity, accuracy, good repeatability and high sensitivity.

In alternative embodiments, provided are polyheterocyclic uncharged bisoxime antidotes, including polyheterocyclic uncharged bisoximes, and methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein. In alternative embodiments, provided are methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain; or, treating, ameliorating or protecting (preventing) an organophosphate toxicity or poisoning or toxic exposure, or for treating, ameliorating or protecting (preventing) organophosphate inhibition of an acetylcholinesterase (AChE) comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein.

Described herein are compounds that reactivate acetylcholinesterase and associated methods of treating organophosphate poisoning.

The application discloses application of pyrroloquinoline quinone (PQQ) or a derivative thereof in preparation of a product for relieving or preventing blue-green algae toxin poisoning of yellow catfish, and belongs to the technical field of aquatic animal feed. The PQQ or the derivative thereof can be used as an aquatic feed additive or a medicine, and is added in an amount of 3-6 mg / kg of feed to feed yellow catfish, so as to effectively relieve pathological damage of the liver of the yellow catfish caused by blue-green algae toxin through various ways such as improving key enzyme activity of liver function, inhibiting liver oxidative stress, relieving inflammatory reaction and reducing structural damage of tissues. The PQQ is a new food raw material approved in China in 2022, has advantages of high safety, good water solubility, moderate cost, similar dosage to that of feed vitamins and the like, and provides a safe, economical and convenient new nutritional intervention strategy for blue-green algae toxin poisoning of the yellow catfish, and has a wide industrial application prospect.

Extract of Saccharomyces Cerevisiae Yeast Cell Walls Rich in β-Glucans for the Prevention of the Effects of the Mycotoxin Deoxynivalenol (DON) The present invention relates to extracts of Saccharomyces cerevisiae yeast cell walls rich in β-glucans, and their use in the prevention of mycotoxicoses caused by the mycotoxin deoxynivalenol, in particular in the prevention of liver and intestinal damage, and the immunotoxic effects caused by this mycotoxin. (no figure)

Products, pharmaceutical compositions, methods of use, and kits are provided for treating a patient suffering from botulinum neurotoxin intoxication. Featured are single-domain variable heavy-chain (VHH) polypeptides (antibodies) that target and neutralize the light chain (LC) protease domains of botulinum neurotoxin (BoNT), i.e., BoNT LC / A and BoNT LC / B, and that advantageously provide late and post-exposure therapy for botulism patients. The LC / A toxin-binding and LC / B toxin-binding VHH polypeptides, which can be recombinantly produced, specifically bind to the LCs of the botulinum neurotoxins to inhibit toxin activity and treat intoxication.

The invention discloses application of sulforaphane, and belongs to the field of medicine. Experiments with mouse AML-12 cells find that when the mouse AML-12 cells are jointly treated with sulforaphane and alpha-amatoxin, the inhibition effect of alpha-amatoxin on the activity of the AML-12 cells can be effectively relieved, the ROS level in the cells can be reduced, cell oxidative stress injury can be relieved, mitochondrial membrane potential reduction can be inhibited, and the mouse AML-12 cells can be treated with raphane and alpha-amatoxin. The sulforaphane can be used for relieving alpha-amatoxin induced mouse AML-12 cell mitochondrial injury, so that the sulforaphane can become a novel medicine for relieving alpha-amatoxin poisoning. The new application of the sulforaphane is found for the first time, and a new substance is provided for preparing the medicine for relieving alpha-amatoxin poisoning.

The invention discloses an application of artemether, artesunate or dihydroartemisinin in preparation of a medicine for preventing or treating aflatoxin B1 poisoning. Belongs to the technical field of medicine. The technical problem of detoxification of aflatoxin B1 is solved. The invention provides an application of artemether, artesunate or dihydroartemisinin in preparation of a medicine for treating aflatoxin B1 poisoning diseases. The body can be promoted to detoxify the AFB1.

The invention belongs to the field of biological medicine, and relates to application of a TRPV4 inhibitor in preparation of a medicine for preventing or treating diseases caused by trimethyltin chloride poisoning. The TRPV4 ion channel inhibitor can remarkably prolong the incubation period of epilepsy caused by trimethyltin chloride poisoning, relieve the severity of epileptic seizure, improve nerve injury and increase the survival rate. Therefore, a new drug target is provided for diagnosis of trimethyltin chloride poisoning and drug research and development, and a new strategy is provided for clinical treatment of trimethyltin chloride poisoning.

The invention relates to the technical field of medical monitoring, and discloses an intelligent early warning method and system for local anesthetic poisoning, and the early warning method comprises the steps: obtaining multi-modal data containing the structural features of a patient, physiological time sequence data and an initial ultrasonic image; based on the ultrasonic image, generating a guide signal representing a space risk and a depth space feature vector in parallel; adaptive attention weighting is carried out on the physiological time sequence data under the guidance of a guidance signal representing the spatial risk, and an adaptive time sequence feature vector is generated; and finally, fusing the structural features, the depth space features and the adaptive time sequence features, determining a poisoning risk level, and performing early warning. By fusing the multi-modal data and utilizing the space risk analyzed by the ultrasonic image to guide the analysis focus of the time series data, the method can capture and predict the weak physiological change of the toxic reaction, thereby overcoming the defects of strong subjectivity and response lagging of the traditional monitoring, and improving the timeliness, accuracy and objectivity of early warning.

Activated carbon compounds for the detoxification of intoxications, including methods of use thereof are provided. Many embodiments provide an approach to detoxifying individuals, including household pets (e.g., dogs and / or cats) from toxic substances. Many embodiments are directed to compositions including activated carbon compounds that can be used to detoxify toxins and other intoxications in an individual. Many embodiments may use activated carbon spheres for detoxification. Many embodiments of compositions can be administered orally. In numerous embodiments, the individual is a human and / or a non-human animal, such as a household pet, including dogs and / or cats.

The invention relates to a biomarker for evaluating progression and prognosis of paraquat poisoning and application of the biomarker. The biomarker is a urine metabolite Inosine. Urine non-target metabonomics analysis shows that the level of the Inosine is low in urine of a patient suffering from early paraquat poisoning, is gradually reduced along with aggravation of the illness state and is remarkably increased in follow-up visit of five years; the prediction efficiency is judged according to the area (AUC) below the working characteristic (ROC) curve of a subject, the Anosine has good efficiency (AUC = 0.806) in judging the progression of paraquat poisoning, has good efficiency (AUC = 0.816) in judging the prognosis aspect in 1-4 days at the early stage of poisoning, and has excellent efficiency (AUC = 0.961) in judging the prognosis aspect in 7-14 days at the progression stage of poisoning. Moreover, the efficiency of the Inosine in predicting the progress and prognosis of paraquat poisoning is better than that of blood routine indexes (such as the number of leukocytes, the percentage of neutrophils and the percentage of lymphocytes) reported in the literature. In conclusion, as the biomarker for prognosis and prognosis of paraquat poisoning, the Inosine shows a good clinical application prospect.

The invention relates to the technical field of human body dietary nutrient dietary fibers. The average molecular weight of the apple gum is 50,000-250,000 Daltons, the average molecular weight of the apple gum can reach 160-50,000 Daltons after being subjected to low molecularization, the apple gum acts in human cells and can prevent toxic cation poisoning, and main methods for preparing oligosaccharide from pectin at present are divided into a physical method, a chemical method and a biological enzyme method. The method comprises the following steps: 1, processing apple peel residues into powder; the method comprises the following steps: heating and drying apple pomace, continuously taking 20-50% of the pomace, mixing the pomace with undried apple juice to 100% by weight, then drying by using a dryer, and repeating the processes of drying and mixing the pomace. And 2, carrying out secondary heating. And heating the powdered apple fibers at 120 DEG C for 30 minutes. And 3, spray drying. The powder is sprayed and dried at a certain rate through a spray dryer according to a ratio of about 3: 1 to about 2: 1, so that microstructures such as powder smell, color, roughness and the like are improved.

The present application relates to the technical field of emergency material scheduling, and particularly relates to a multi-level inventory collaborative allocation method for medical materials under a sudden health event. The multi-level inventory collaborative allocation method for medical materials under a sudden health event comprises the following steps: S1: real-time acquisition of organophosphorus exposure data of a sudden health event area, construction of exposure concentration spatiotemporal distribution and spatiotemporal disability surface according to the organophosphorus exposure data; S2: acquisition of total drug demand of the sudden health event area and self-protection demand threshold of each inventory node according to the exposure concentration spatiotemporal distribution and the spatiotemporal disability surface, and acquisition of initial allocation data based on the self-protection demand threshold of each inventory node. The present application embeds the toxicology time-dose effect into the multi-level inventory collaborative decision, so that the allocation scheme can match the minute-level rescue window after organophosphorus poisoning, and the risk of irreversible injury and death caused by drug delay is significantly reduced.

This invention provides the use of β-glucuronidase in the preparation of an antidote for the prevention and / or treatment of zearalenone poisoning, and an antidote for the prevention and / or treatment of zearalenone poisoning, belonging to the field of feed additives. This invention, for the first time from the perspective of in vivo exposure control in animals, proposes a method to reduce the overall ZEN exposure level by inhibiting the activity of intestinal bacterial β-glucuronidase. The technical path is clear and highly operable. This is achieved through in vivo toxicokinetic parameters (AUC, C...). max Changes in (and / or MRT) objectively reflect a reduction in ZEN exposure levels in vivo, and the technical effects are quantifiable and verifiable. The β-glucuronidase inhibitors used in this invention are widely available, particularly suitable for the field of feed additives, and have promising application prospects.

The application discloses a chrysanthemum stem and leaf active extract with improved fish heavy metal poisoning and a preparation method thereof. The chrysanthemum stem and leaf active extract is compounded by a chrysanthemum stem and leaf crude polysaccharide part and a chrysanthemum stem and leaf phenolic ketone part. The pharmacodynamic experiment results show that the chrysanthemum stem and leaf active extract has a good improvement effect on fish heavy metal poisoning. The chrysanthemum stem and leaf active extract can not only improve pathological changes such as heart and liver granuloma, kidney tubular cast, intestinal cavity expansion and gill lamella shedding caused by heavy metal poisoning, but also improve metabolic dysfunction such as taurine and hypotaurine metabolic disorder, pyruvic acid metabolic abnormality, tricarboxylic acid cycle disorder, glycolysis and gluconeogenesis disorder caused by heavy metal poisoning. The preparation method has reasonable whole preparation process design, can realize waste utilization, extends a chrysanthemum industry chain, expands a chrysanthemum resource utilization approach, is helpful to quality improvement and efficiency increase and green development of the chrysanthemum industry, and provides support for prevention and treatment of heavy metal poisoning.

The invention discloses a neutralizing antibody targeting abrus precatorius toxin and application of the neutralizing antibody. Specifically disclosed is a monoclonal antibody or an antigen-binding fragment thereof targeting an abrine toxin, comprising a heavy chain variable region (SEQ ID NO: 1) and a light chain variable region (SEQ ID NO: 2). The monoclonal antibody has high affinity, can specifically target an A chain of Abrin-a toxin, can significantly inhibit Abrin-a induced cytotoxicity, has a significant protection effect on cells attacked by abrus precatorius toxin, can provide long-acting protection, and has good neutralizing ability. The monoclonal antibody can be prepared into products such as a therapeutic drug and a diagnostic drug for abrus precatorius toxin poisoning, a detection kit for abrus precatorius toxin and the like clinically. Based on a unique action mechanism and a remarkable treatment effect, the invention provides a novel biological preparation for preventing and treating Abrin poisoning, and has a wide clinical application prospect in the fields of first-aid medicine and biological defense.

The application belongs to the field of biological products, and relates to a single-domain antibody of epsilon toxin of Clostridium perfringens and application thereof. The application specifically discloses a single-domain antibody of epsilon toxin of Clostridium perfringens, and the amino acid sequence of the single-domain antibody is shown in SEQ ID NO. 16. The single-domain antibody can neutralize epsilon toxin in vivo, can treat animals poisoned by epsilon toxin, and has application prospect.

The invention discloses an application of butyric acid or a derivative thereof in preparation of a zearalenone reproductive toxicity resisting preparation. Also disclosed is an antitoxic feed composition comprising a specific base ration and a butyric acid derivative. In-vivo and in-vitro tests and molecular mechanism researches prove that butyric acid and derivatives thereof (such as tributyrin and sodium butyrate) can effectively relieve zearalenone (ZEN)-induced animal ovary injury. The action mechanism of the traditional Chinese medicine composition is that follicular atresia is reduced, the balance of reproductive hormones (such as GnRH, E2, AMH and P4) is recovered, and clinical symptoms caused by ZEN poisoning such as vulva swelling and the like are relieved by activating a BMPs / SMAD signal channel, especially up-regulating SMAD4 protein expression and inhibiting oxidative stress (ROS) and Caspase-3 mediated granular cell apoptosis.

The invention discloses application of a compound BRD4780 as an anti-shiga toxin protective agent and related products thereof, and provides an anti-shiga toxin pharmaceutical composition and a pharmaceutical preparation, it is found for the first time that the compound BRD4780 can be used as the effective anti-shiga toxin protective agent, the BRD4780 can effectively inhibit the toxicity of shiga toxin I and shiga toxin II, and the compound BRD4780 can effectively inhibit the toxicity of shiga toxin I and shiga toxin II. The BRD4780 can be applied to preparation of anti-shiga toxin drugs, an effective candidate drug is provided for prevention, treatment, alleviation or improvement of shiga toxin poisoning related diseases or symptoms, and reference is provided for new application clinical research of the BRD4780 drug at the same time.

A composition comprising isolated Streptococcus pyogenes extracellular vesicles (EVs) suspended in a vehicle, for use in eliciting an adaptive immune response against group A streptococcus (GAS) in a subject. The EVs may comprise antigens MtsA, Spy_1228, PrsA1, PrsA2, SPy_0319 and / or GlnP. A composition comprising isolated S. pyogenes protein(s) MtsA, SPy_1228, PrsA1, PrsA2, GlnP, SPy_0319 and / or MalX. The compositions may be used for inducing protective immunity in a subject against a GAS infection, such as streptococcal pharyngitis, scarlet fever, impetigo, streptococcal meningitis, streptococcal sinusitis, necrotizing fasciitis, cellulitis, streptococcal toxic shock syndrome, rheumatic fever and post-streptococcal glomerulonephritis.

The invention relates to the technical field of biological analysis and detection, in particular to a cucurbituril derivative for drug detection, a preparation method and application of the cucurbituril derivative, a detection probe containing the cucurbituril derivative, a drug for treating acute poisoning of drugs and a drug detection method. On the basis of retaining the original supramolecular recognition characteristic of cucurbituril, the derivative introduces an o-cyanobenzaldehyde (CNBA) functional group; the obtained compound can realize high-efficiency covalent capture on common drugs and metabolites thereof (such as primary amine groups in molecules of methylamphetamine and metabolites thereof, 3, 4-methylenedioxy methylamphetamine, ketamine and metabolites thereof, fluazinone and metabolites thereof, phencyclohexylpiperidine, cathinone and the like); therefore, rapid and high-sensitivity visual detection of the target object is realized, and the method can be used for antagonistic treatment of acute poisoning caused by related drugs and metabolites.

This invention provides a multi-site mutant of cocaine esterase, its protein dimer, and its applications. By mutating cysteine ​​(C) at position 107 to serine (S), and cysteine ​​(C) at position 551 to serine (S), arginine (R), or alanine (A), it achieves efficient and uniform cross-linking with BMOE while maintaining cocaine catalytic activity, forming a structurally stable dimer. This modification significantly improves the enzyme's thermostability and prolongs its half-life in vivo. The protein dimer is suitable for the treatment and prevention of acute cocaine poisoning.

The application provides an organic phosphorus poisoning relief composite enzyme and application thereof, and belongs to the technical field of biological medicine. The organic phosphorus poisoning relief composite enzyme provided by the application comprises polyethylene glycol modified organic phosphorus hydrolase and butyrylcholine esterase, and the mass ratio of the polyethylene glycol modified organic phosphorus hydrolase to the butyrylcholine esterase is (4-8):(2500-10000). The rat acute organic phosphorus poisoning treatment result shows that the organic phosphorus poisoning relief composite enzyme provided by the application fully plays the efficient catalytic hydrolysis capacity of the enzyme as a catalyst, efficiently removes organic phosphorus and acetylcholine by using the organic phosphorus hydrolase and the butyrylcholine esterase respectively, provides a drug combination for treating the cause for the organic phosphorus poisoning injury first aid, and the treatment effect is significantly better than that of the single drug.

A composition comprising isolated Streptococcus pyogenes extracellular vesicles (EVs) suspended in a vehicle, for use in eliciting an adaptive immune response against group A streptococcus (GAS) in a subject. The EVs may comprise antigens MtsA, Spy_1228, PrsA1, PrsA2, SPy_0319 and / or GlnP. A composition comprising isolated S. pyogenes protein(s) MtsA, SPy_1228, PrsA1, PrsA2, GlnP, SPy_0319 and / or MalX. The compositions may be used for inducing protective immunity in a subject against a GAS infection, such as streptococcal pharyngitis, scarlet fever, impetigo, streptococcal meningitis, streptococcal sinusitis, necrotizing fasciitis, cellulitis, streptococcal toxic shock syndrome, rheumatic fever and post-streptococcal glomerulonephritis.

Breath analyser for detecting poisoning of an individual with a toxic compound, comprising an array of graphene varactor sensors elements (302). At least some of the graphene sensor elements (302) are modified with metalloporphyrin compounds. Poisoning is detected by classifying the response pattern of the graphene varactor sensor elements (302).

The invention discloses a sepsis patient diagnosis and prognosis system based on a neutrophil toxicity index. According to the sepsis patient diagnosis and prognosis system based on the neutrophil toxicity index provided by the invention, only a small amount of blood is required to be subjected to sample suction, sheet pushing, dyeing, drying and sheet reading according to a standardized process, and an optical microscopic image of a blood smear is obtained for cell morphology analysis. The number of the neutrophils containing the poisoning particles can be clearly quantified, the toxicity index is generated according to the neutrophils, and the method is rapid, simple and visual. The significance of TI, CRP and PCT in prompting diagnosis and prognosis of sepsis patients is analyzed through ROC curve comparison, the area of TI under the curve is larger than that of CRP and PCT, and the method is high in sepsis early-stage risk prediction and diagnosis efficacy.

The present disclosure provides certain LATS1 and / or LATS2 inhibitors, including selective LATS1 / LATS2 inhibitors and dual LATS1 / LATS2 and AKT inhibitors that are useful for the treatment of wounds, of diseases that would benefit from organ or cellular regeneration, of cancer, and of heavy metal poisoning, as well as for promoting ex vivo growth of a cell line or a cell product and for accelerating tissue growth ex vivo. Also provided are pharmaceutical compositions containing such compounds and methods of using such compounds.