64 results about "Toxication" patented technology

The invention discloses a method for determining a quaternary ammonium salt pesticide in blood by using liquid chromatography-mass spectrometry, which comprises the following steps of: drawing a whole blood (plasma and serum) matrix labeling standard working curve through liquid chromatography-mass spectrometry analysis; obtaining peak areas of quantitative ion peaks of target objects containing paraquat, aquacide, diguat, chlormequat and mepiperidine with different concentrations and peak areas of quantitative ion peaks of internal standard substances, and further drawing a standard working curve; and performing liquid chromatography-mass spectrometry analysis on the whole blood sample under the same condition to obtain the peak area of a target substance quantitative ion peak and the peak area of an internal standard substance quantitative ion peak in the whole blood test solution, and calculating the content of each quaternary ammonium salt pesticide according to the standard working curve. The method can be used for simultaneously detecting the content of five quaternary ammonium salt pesticides in the whole blood of the poisoned patient simply, conveniently and quickly, and has the characteristics of simplicity, accuracy, good repeatability and high sensitivity.

In alternative embodiments, provided are polyheterocyclic uncharged bisoxime antidotes, including polyheterocyclic uncharged bisoximes, and methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein. In alternative embodiments, provided are methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain; or, treating, ameliorating or protecting (preventing) an organophosphate toxicity or poisoning or toxic exposure, or for treating, ameliorating or protecting (preventing) organophosphate inhibition of an acetylcholinesterase (AChE) comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein.

The application discloses application of pyrroloquinoline quinone (PQQ) or a derivative thereof in preparation of a product for relieving or preventing blue-green algae toxin poisoning of yellow catfish, and belongs to the technical field of aquatic animal feed. The PQQ or the derivative thereof can be used as an aquatic feed additive or a medicine, and is added in an amount of 3-6 mg / kg of feed to feed yellow catfish, so as to effectively relieve pathological damage of the liver of the yellow catfish caused by blue-green algae toxin through various ways such as improving key enzyme activity of liver function, inhibiting liver oxidative stress, relieving inflammatory reaction and reducing structural damage of tissues. The PQQ is a new food raw material approved in China in 2022, has advantages of high safety, good water solubility, moderate cost, similar dosage to that of feed vitamins and the like, and provides a safe, economical and convenient new nutritional intervention strategy for blue-green algae toxin poisoning of the yellow catfish, and has a wide industrial application prospect.

Extract of Saccharomyces Cerevisiae Yeast Cell Walls Rich in β-Glucans for the Prevention of the Effects of the Mycotoxin Deoxynivalenol (DON) The present invention relates to extracts of Saccharomyces cerevisiae yeast cell walls rich in β-glucans, and their use in the prevention of mycotoxicoses caused by the mycotoxin deoxynivalenol, in particular in the prevention of liver and intestinal damage, and the immunotoxic effects caused by this mycotoxin. (no figure)

Products, pharmaceutical compositions, methods of use, and kits are provided for treating a patient suffering from botulinum neurotoxin intoxication. Featured are single-domain variable heavy-chain (VHH) polypeptides (antibodies) that target and neutralize the light chain (LC) protease domains of botulinum neurotoxin (BoNT), i.e., BoNT LC / A and BoNT LC / B, and that advantageously provide late and post-exposure therapy for botulism patients. The LC / A toxin-binding and LC / B toxin-binding VHH polypeptides, which can be recombinantly produced, specifically bind to the LCs of the botulinum neurotoxins to inhibit toxin activity and treat intoxication.

The invention discloses an application of artemether, artesunate or dihydroartemisinin in preparation of a medicine for preventing or treating aflatoxin B1 poisoning. Belongs to the technical field of medicine. The technical problem of detoxification of aflatoxin B1 is solved. The invention provides an application of artemether, artesunate or dihydroartemisinin in preparation of a medicine for treating aflatoxin B1 poisoning diseases. The body can be promoted to detoxify the AFB1.

The invention belongs to the field of biological medicine, and relates to application of a TRPV4 inhibitor in preparation of a medicine for preventing or treating diseases caused by trimethyltin chloride poisoning. The TRPV4 ion channel inhibitor can remarkably prolong the incubation period of epilepsy caused by trimethyltin chloride poisoning, relieve the severity of epileptic seizure, improve nerve injury and increase the survival rate. Therefore, a new drug target is provided for diagnosis of trimethyltin chloride poisoning and drug research and development, and a new strategy is provided for clinical treatment of trimethyltin chloride poisoning.

The invention relates to the technical field of medical monitoring, and discloses an intelligent early warning method and system for local anesthetic poisoning, and the early warning method comprises the steps: obtaining multi-modal data containing the structural features of a patient, physiological time sequence data and an initial ultrasonic image; based on the ultrasonic image, generating a guide signal representing a space risk and a depth space feature vector in parallel; adaptive attention weighting is carried out on the physiological time sequence data under the guidance of a guidance signal representing the spatial risk, and an adaptive time sequence feature vector is generated; and finally, fusing the structural features, the depth space features and the adaptive time sequence features, determining a poisoning risk level, and performing early warning. By fusing the multi-modal data and utilizing the space risk analyzed by the ultrasonic image to guide the analysis focus of the time series data, the method can capture and predict the weak physiological change of the toxic reaction, thereby overcoming the defects of strong subjectivity and response lagging of the traditional monitoring, and improving the timeliness, accuracy and objectivity of early warning.

This invention provides the use of β-glucuronidase in the preparation of an antidote for the prevention and / or treatment of zearalenone poisoning, and an antidote for the prevention and / or treatment of zearalenone poisoning, belonging to the field of feed additives. This invention, for the first time from the perspective of in vivo exposure control in animals, proposes a method to reduce the overall ZEN exposure level by inhibiting the activity of intestinal bacterial β-glucuronidase. The technical path is clear and highly operable. This is achieved through in vivo toxicokinetic parameters (AUC, C...). max Changes in (and / or MRT) objectively reflect a reduction in ZEN exposure levels in vivo, and the technical effects are quantifiable and verifiable. The β-glucuronidase inhibitors used in this invention are widely available, particularly suitable for the field of feed additives, and have promising application prospects.

The application discloses a chrysanthemum stem and leaf active extract with improved fish heavy metal poisoning and a preparation method thereof. The chrysanthemum stem and leaf active extract is compounded by a chrysanthemum stem and leaf crude polysaccharide part and a chrysanthemum stem and leaf phenolic ketone part. The pharmacodynamic experiment results show that the chrysanthemum stem and leaf active extract has a good improvement effect on fish heavy metal poisoning. The chrysanthemum stem and leaf active extract can not only improve pathological changes such as heart and liver granuloma, kidney tubular cast, intestinal cavity expansion and gill lamella shedding caused by heavy metal poisoning, but also improve metabolic dysfunction such as taurine and hypotaurine metabolic disorder, pyruvic acid metabolic abnormality, tricarboxylic acid cycle disorder, glycolysis and gluconeogenesis disorder caused by heavy metal poisoning. The preparation method has reasonable whole preparation process design, can realize waste utilization, extends a chrysanthemum industry chain, expands a chrysanthemum resource utilization approach, is helpful to quality improvement and efficiency increase and green development of the chrysanthemum industry, and provides support for prevention and treatment of heavy metal poisoning.

The invention discloses a neutralizing antibody targeting abrus precatorius toxin and application of the neutralizing antibody. Specifically disclosed is a monoclonal antibody or an antigen-binding fragment thereof targeting an abrine toxin, comprising a heavy chain variable region (SEQ ID NO: 1) and a light chain variable region (SEQ ID NO: 2). The monoclonal antibody has high affinity, can specifically target an A chain of Abrin-a toxin, can significantly inhibit Abrin-a induced cytotoxicity, has a significant protection effect on cells attacked by abrus precatorius toxin, can provide long-acting protection, and has good neutralizing ability. The monoclonal antibody can be prepared into products such as a therapeutic drug and a diagnostic drug for abrus precatorius toxin poisoning, a detection kit for abrus precatorius toxin and the like clinically. Based on a unique action mechanism and a remarkable treatment effect, the invention provides a novel biological preparation for preventing and treating Abrin poisoning, and has a wide clinical application prospect in the fields of first-aid medicine and biological defense.

The application belongs to the field of biological products, and relates to a single-domain antibody of epsilon toxin of Clostridium perfringens and application thereof. The application specifically discloses a single-domain antibody of epsilon toxin of Clostridium perfringens, and the amino acid sequence of the single-domain antibody is shown in SEQ ID NO. 16. The single-domain antibody can neutralize epsilon toxin in vivo, can treat animals poisoned by epsilon toxin, and has application prospect.

The invention discloses an application of butyric acid or a derivative thereof in preparation of a zearalenone reproductive toxicity resisting preparation. Also disclosed is an antitoxic feed composition comprising a specific base ration and a butyric acid derivative. In-vivo and in-vitro tests and molecular mechanism researches prove that butyric acid and derivatives thereof (such as tributyrin and sodium butyrate) can effectively relieve zearalenone (ZEN)-induced animal ovary injury. The action mechanism of the traditional Chinese medicine composition is that follicular atresia is reduced, the balance of reproductive hormones (such as GnRH, E2, AMH and P4) is recovered, and clinical symptoms caused by ZEN poisoning such as vulva swelling and the like are relieved by activating a BMPs / SMAD signal channel, especially up-regulating SMAD4 protein expression and inhibiting oxidative stress (ROS) and Caspase-3 mediated granular cell apoptosis.

A composition comprising isolated Streptococcus pyogenes extracellular vesicles (EVs) suspended in a vehicle, for use in eliciting an adaptive immune response against group A streptococcus (GAS) in a subject. The EVs may comprise antigens MtsA, Spy_1228, PrsA1, PrsA2, SPy_0319 and / or GlnP. A composition comprising isolated S. pyogenes protein(s) MtsA, SPy_1228, PrsA1, PrsA2, GlnP, SPy_0319 and / or MalX. The compositions may be used for inducing protective immunity in a subject against a GAS infection, such as streptococcal pharyngitis, scarlet fever, impetigo, streptococcal meningitis, streptococcal sinusitis, necrotizing fasciitis, cellulitis, streptococcal toxic shock syndrome, rheumatic fever and post-streptococcal glomerulonephritis.

This invention provides a multi-site mutant of cocaine esterase, its protein dimer, and its applications. By mutating cysteine ​​(C) at position 107 to serine (S), and cysteine ​​(C) at position 551 to serine (S), arginine (R), or alanine (A), it achieves efficient and uniform cross-linking with BMOE while maintaining cocaine catalytic activity, forming a structurally stable dimer. This modification significantly improves the enzyme's thermostability and prolongs its half-life in vivo. The protein dimer is suitable for the treatment and prevention of acute cocaine poisoning.

The application provides an organic phosphorus poisoning relief composite enzyme and application thereof, and belongs to the technical field of biological medicine. The organic phosphorus poisoning relief composite enzyme provided by the application comprises polyethylene glycol modified organic phosphorus hydrolase and butyrylcholine esterase, and the mass ratio of the polyethylene glycol modified organic phosphorus hydrolase to the butyrylcholine esterase is (4-8):(2500-10000). The rat acute organic phosphorus poisoning treatment result shows that the organic phosphorus poisoning relief composite enzyme provided by the application fully plays the efficient catalytic hydrolysis capacity of the enzyme as a catalyst, efficiently removes organic phosphorus and acetylcholine by using the organic phosphorus hydrolase and the butyrylcholine esterase respectively, provides a drug combination for treating the cause for the organic phosphorus poisoning injury first aid, and the treatment effect is significantly better than that of the single drug.

A composition comprising isolated Streptococcus pyogenes extracellular vesicles (EVs) suspended in a vehicle, for use in eliciting an adaptive immune response against group A streptococcus (GAS) in a subject. The EVs may comprise antigens MtsA, Spy_1228, PrsA1, PrsA2, SPy_0319 and / or GlnP. A composition comprising isolated S. pyogenes protein(s) MtsA, SPy_1228, PrsA1, PrsA2, GlnP, SPy_0319 and / or MalX. The compositions may be used for inducing protective immunity in a subject against a GAS infection, such as streptococcal pharyngitis, scarlet fever, impetigo, streptococcal meningitis, streptococcal sinusitis, necrotizing fasciitis, cellulitis, streptococcal toxic shock syndrome, rheumatic fever and post-streptococcal glomerulonephritis.

Provided herein are methods of immunizing against, treating or preventing streptococcal toxic shock syndrome in a subject, by administration of a group A streptococcus M protein, inclusive of fragments, variants or derivatives thereof, or an antibody that binds, or is raised against the M protein and optionally a group A streptococcus superantigen protein, inclusive of fragments, variants or derivatives thereof, or an antibody or antibody fragment that binds, or is raised against, the superantigen protein.

The invention relates to the technical field of pharmaceutical biology, aims to solve the problem of lack of specific detoxification drugs for treating glyphosate poisoning clinically, and particularly provides application of chlorogenic acid in preparation of drugs for treating glyphosate-induced renal toxicity diseases. The drug mechanism of the chlorogenic acid is as follows: glyphosate-induced renal toxicity is antagonized through targeted renal injury biomarkers, wherein the renal injury biomarkers comprise TIM-1, TIMP-2, IGFBP7 and LCN2. The invention also provides a preparation for preventing and / or relieving and / or treating glyphosate-induced renal toxicity diseases, wherein the preparation contains chlorogenic acid. The preparation can be a medicine, a pharmaceutical composition, a health care product, a food or an additive. The medicine prepared by the invention can be used for remarkably reducing the expression levels of TIM-1, TIMP-2, IGFBP7 and LCN2 of the GLY-induced renal injury. In addition, oxidative stress (LCN2), cell cycle arrest (TIMP-2 / IGFBP7) and inflammatory reaction (TIM-1) can be synchronously and accurately inhibited, and the curative effect is superior to that of a current single-channel medicine.

A method is described for the rapid clearance of carbon monoxide (CO) from CO-bound hemoglobin, myoglobin, and cytochrome c oxidase in subjects with CO poisoning. The disclosed therapeutic method involves the use of rationally designed and engineered regulator of CO metabolism (RcoM) proteins and pharmaceutical compositions thereof, which scavenge CO from poisoned tissues. The recombinant RcoM composition is infused into the blood, where it rapidly sequesters CO and limits the toxic effects of CO on cellular respiration, oxygen transport, and oxygen utilization.

The invention belongs to the field of biological products, and relates to an epsilon toxin single-domain antibody of clostridium perfringens and application of the epsilon toxin single-domain antibody. The invention particularly discloses a single-domain antibody of epsilon toxin of clostridium perfringens, wherein the amino acid sequence of the single-domain antibody is shown as SEQ ID NO.18. The single-domain antibody can neutralize epsilon toxin in vivo, can treat animals poisoned by the epsilon toxin, and has an application prospect.

The present disclosure relates essentially to a supplement, the function of which is to enhance immunity, prevent various diseases and assist in therapeutic treatments of various diseases, said supplement includes: 100 mg of lipoic acid, 1 mg of astaxanthin, 10 mg of glutathione, 5 mg of resveratrol, 5 mg of lycopene, 50 mg of epigallocatechin, 300 mg of curcumin, 400 mg of quercetin, 10 mg of coenzyme Q1 and 100 mg of dry açaí extract; formulated with the intention of combining substances that serve functions of assisting in the treatment of various diseases, such as: lipoic acid, which helps in the prevention of liver damage from intoxication, and can be used as a coadjuvant for the treatment of liver cirrhosis due to alcoholism and a coadjuvant for the treatment of diabetes and cataracts.

The present application relates to the technical field of biological analysis detection, and particularly relates to cucurbituril derivatives for drug detection, a preparation method and application of the cucurbituril derivatives, a detection probe containing the cucurbituril derivatives, a drug for treating drug acute poisoning, and a method for drug detection. The derivative introduces a functional group of o-cyanobenzaldehyde (CNBA) on the basis of retaining the original supramolecular recognition characteristics of cucurbituril, and the obtained compound can realize efficient covalent capture on the primary amine groups in common drugs and metabolites (such as methyl phenylpropylamine and its metabolites, 3,4-methylenedioxy methyl phenylpropylamine, ketamine and its metabolites, fluoroamine and its metabolites, phenylcyclohexylpiperidine, and casiketone molecules), so as to realize rapid, high-sensitivity and visual detection on the above target objects, and can be used for antagonistic treatment of acute poisoning caused by related drugs and metabolites.

Certain embodiments provide an isolated antibody or fragment thereof having affinity for fentanyl or analog thereof, CA as well as methods of use thereof. Certain embodiments provide a method for treating fentanyl or analog related overdose, poisoning, or disorder.

The invention provides a nicotine degrading enzyme mutant as well as a preparation method and application thereof. The invention provides a recombinant nicotine degrading enzyme mutant. On the basis of NicA2 with an amino acid sequence as shown in SEQ ID NO: 1, mutation is carried out at any one of the following positions: the 48th position, the 379th position, the 189th position, the 208th position, the 234th position, the 274th position, the 317th position, the 369th position, the 370th position, the 362th position, the 396th position, the 462th position and the 479th position. Compared with a wild type nicotine degrading enzyme, the mutant has the advantages that the nicotine degrading activity is obviously improved, and the mutant has higher application potential in the aspects of further preparing a medicine for treating nicotine addiction or nicotine poisoning and improving the nicotine degrading activity and stability.

The present disclosure provides a 3-phenoxybenzoic acid-glucuronic acid conjugate, and a preparation method and use thereof, and belongs to the technical field of pesticide detection. Compared with 3-phenoxybenzoic acid, the 3-phenoxybenzoic acid-glucuronic acid conjugate provided by the present disclosure features structural stability, high specificity, long limit of detection, and high content in urine, and can better serve as a marker that identifies whether an organism is killed due to pyrethroid pesticide poisoning. Namely, the 3-phenoxybenzoic acid-glucuronic acid conjugate can detect whether a toxicant (pyrethroid pesticides) is taken antemortem or exposed postmortem, and has an excellent application prospect in pyrethroid pesticide detection. The present disclosure provides a preparation method of a 3-phenoxybenzoic acid-glucuronic acid conjugate. The preparation method provided by the present disclosure features high product yield, simple operation, wide raw material sources, low costs, and suitability for industrial production.

The present invention relates to a compound of formula (I) or a stereoisomer, or tautomer, wherein moiety A, R1, and R2 have the same meaning as that defined in the claims and the description. The present invention also relates to the use of such compounds for the prevention and / or treatment of a disease associated with ferroptosis and / or oxytosis such as liver disease, chronic kidney disease, lung disease, ocular surface diseases, wound healing, multiple organ dysfunction syndrome, neurological disease, acute renal failure, ischemia- reperfusion injury, sepsis, prevention of transplant rejection, iron toxicity, iron metabolism- related disease and genetic disorder of GPX4. The present invention also provides pharmaceutical compositions comprising such compounds, as well as the use of the compounds as a medicament and methods of prevention and / or treatment of such diseases.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an FBXW7 gene in relieving poisoning symptoms caused by vomitoxin and a method. The invention provides an application of a biological material for positively regulating and controlling an FBXW7 gene and / or positively regulating and controlling the FBXW7 gene. The application comprises an application in preparing a medicine for relieving a poisoning symptom caused by vomitoxin; and the login number of the FBXW7 gene in an NCBI (National Center of Biotechnology Information) database is XM021101502.1. The invention finds that overexpression of the FBXW7 gene can inhibit DON-induced IPEC-J2 cell ferroptosis, and provides a new target and test basis for relieving DON-induced pig intestine epithelial cell ferroptosis and improving pig performance.

The invention relates to the technical field of marine organisms, in particular to a nucleic acid aptamer modified nerve extracellular vesicle and application thereof in prevention and treatment of marine guanamine neurotoxin poisoning. According to the invention, extracellular vesicles (nEVs) and a nucleic acid aptamer (Apt) of tetrodotoxin (TTX) are coupled to construct a guanamine toxoid nano antagonism system Apt-nEVs. According to the present invention, the Apt-nEVs retains the natural Na < + > channel on the nerve cell membrane, can synergistically provide the dual detoxification function of the coupling aptamer and the natural Na < + > channel to identify and trap TTX, can achieve the efficient detoxification of TTX in the non-cell system and the cell level, and can significantly protect the cell oxidative stress injury caused by TTX. In addition, the aptamer coupled extracellular vesicles (Apt-nEVs) obtained in the invention can also provide thought and reference for construction of a more broad-spectrum marine biotoxin antagonism system.

The invention discloses a pyrazolone compound as well as a preparation method and application thereof. The compound has a structural formula as shown in a formula I, wherein X is selected from N or O; r1 is selected from thienyl, naphthyl, phenyl or phenyl substituted by at least one of C1-6 alkyl, C1-6 alkoxy and halogen; r2 is selected from alkyl of C1-6, phenyl or phenyl substituted by alkoxy of C1-6; r3 is selected from H and alkyl of C1-6; and R4 is selected from phenyl or phenyl substituted by at least one of C1-6 alkyl, C1-6 alkoxy and halogen. The invention provides a series of pyrazolone compounds with novel structures, and the compounds can protect nematodes and reduce damage of formaldehyde to the nematodes, so that the survival rate of the nematodes is improved. The compound can be used for preparing medicines for treating and / or preventing Parkinson's disease, senile dementia or formaldehyde poisoning.