200 results about "Toxication" patented technology

The invention belongs to the technical field of bioengineering, and particularly relates to a zearalenone toxin degrading enzyme variant and application thereof. The amino acid sequence of the zearalenone toxin degrading enzyme is as shown in SEQ ID NO.2, or the zearalenone toxin degrading enzyme with the amino acid sequence as shown in SEQ ID NO.2 is obtained through amino acid mutation. The zearalenone toxin degrading enzyme mutant is obtained through mutation screening, and compared with a wild type, the zearalenone toxin degrading enzyme mutant has the advantages that the zearalenone degrading capability is obviously improved; besides, the zearalenone toxin degrading enzyme is expressed by using alfalfa, the zearalenone toxin degrading enzyme with biological activity is easy to obtain, and the zearalenone toxin degrading enzyme has certain application potential in prevention and treatment of animal poisoning caused by zearalenone toxin pollution in agriculture and animal husbandry production.

The invention belongs to the technical field of medicines, and particularly relates to an FRET (Fluorescence Resonance Energy Transfer)-based botulinum toxin A and E detection molecular sensor and application thereof. The invention accidentally finds that the BoNT / A or BoNT / E biological activity detection molecular sensor is prepared by taking EGFP / DsRED as donor and receptor fluorophores and SNAP-25 (141-206aa) as a connexon through an after-ripening process, and the detection molecular sensor has the characteristics of high signal-to-noise ratio, rapidness, simplicity, convenience, sensitivity, good accuracy, high sensitivity, good repeatability and high stability. The biological activity or content detection on BoNT / A or BoNT / E at a molecular level can be realized; the kit can be applied to detection of the biological activity of the light chain in the preparation process of BoNT / A or BoNT / E extracted by a recombination or traditional method, can also be used for in-vitro screening of botulinum toxin inhibitors, and can also be used for poisoning diagnosis of BoNT / A or BoNT / E.

Described herein are compounds that reactivate acetylcholinesterase and associated methods of treating organophosphate poisoning.

The invention provides a preparation method of (S)-oxetane-2-methylamine. The preparation method of (S)-oxetane-2-methylamine comprises the following steps: (1) carrying out ring closing reaction on a compound A under an alkaline condition to obtain a compound B; (2) reacting the compound B with dibenzylamine to obtain a compound C; (3) reacting the compound C with a reducing agent to obtain a compound D; (4) reacting the compound D with paratoluensulfonyl chloride to obtain a compound E; (5) reacting the compound E with alkali to obtain a compound F; and (6) carrying out a reaction on the compound F and palladium on carbon to obtain the (S)-oxetane-2-methylamine. According to the preparation method provided by the invention, ring expansion reaction using trimethylsulfoxide iodide in the prior art is avoided, the problem that residual sulfur elements cause poisoning of a palladium-carbon catalyst is solved, meanwhile, sodium azide is also avoided, and the preparation method is safe and reliable in process, high in chiral purity and yield and suitable for industrial large-scale production.

Provided are a Lactobacillus paracasei FMBL L23249 FJX and the use thereof. The Lactobacillus paracasei FMBL L23249 FJX is deposited in the China Center for Type Culture Collection on June 26, 2023, with a deposit number of CCTCC NO: M 20231097. The Lactobacillus paracasei FMBL L23249 FJX can be rapidly proliferated by means of efficiently utilizing a Cicer arietinum L. extract, and can be used for preparing Cicer arietinum L. yogurt. The Lactobacillus paracasei FMBL L23249 FJX can also adsorb heavy metals. Further provided is the use of the Lactobacillus paracasei FMBL L23249 FJX in the preparation of a drug, food, health care product or cosmetic for preventing and treating heavy metal poisoning. The Lactobacillus paracasei FMBL L23249 FJX has high adsorbability for the heavy metals lead and cadmium, with the adsorption rate for lead reaching 99.87%, and the adsorption rate for cadmium reaching 82.74%. The Lactobacillus paracasei FMBL L23249 FJX has good acid resistance, cholate resistance, drug resistance, self-aggregation and hydrophobicity, can inhibit pathogenic bacteria, has an antioxidant capacity, and has wide application prospects.

The invention discloses application of trans-anisd in relieving toxicity of zearalenone in animals, and belongs to the technical field of prevention and treatment of mycotoxin. The invention provides an application of trans-anisd in preparation of a medicine or feed for relieving zearalenone poisoning of animals. The trans-anisd is proved to be capable of effectively relieving piglet reproductive toxicity caused by zearalenone, improving uterine organ indexes and ovarian organ indexes and ensuring piglet health and reproductive system development, and a new method is provided for zearalenone detoxification.

The invention relates to an application of beta-nicotinamide mononucleotide (NMN) in prevention and treatment of liver injury caused by aflatoxin B1 (AFB1), the NMN has a good protection effect on the liver injury caused by exposure of the AFB1, and particularly, the serum transaminase activity and liver tissue pathological injury of a mouse using an NMN + AFB1 group are improved compared with those of a mouse in the AFB1 group, and the NMN has a good protection effect on the liver injury caused by exposure of the AFB1 group. Namely, the NMN has a certain protection effect on the mouse liver injury induced by exposure of the AFB1; the content of IL-6, IL-1beta and TNF-alpha in the liver of a mouse in an AFB1 group is remarkably increased, and the expression of IL-6, IL-1beta and TNF-alpha in the liver of the mouse infected with AFB1 is remarkably reduced by the NMN, namely, the NMN relieves the inflammatory response induced by the AFB1. The NMN can effectively relieve the development of mouse AFB1 subchronic poisoning and gradually recover the mouse AFB1 subchronic poisoning to a normal level, so that a new clue and an experimental basis are provided for research and development of an additive or a medicine for resisting AFB1 poisoning.

The invention provides a compound preparation for reducing the toxic effect of zearalenone of bred animals as well as a preparation method and application of the compound preparation, and belongs to the technical field of medicines for livestock and poultry. The invention provides a compound preparation for reducing the toxic effect of zearalenone of bred animals. The compound preparation is prepared from the following components in parts by weight: 40 to 50 parts of total flavonoids of radix astragali seu hedysari, 0.1 to 0.4 part of selenium-enriched yeast, 10 to 15 parts of modified montmorillonite and 1 to 4 parts of vitamin C. The four components in the compound preparation are mutually matched and synergistically synergized to reduce the toxic effect of zearalenone and resist zearalenone poisoning of bred animals, and the compound preparation has high safety, so that the production performance of the bred animals and the quality of animal-derived products are improved, the green and healthy development of animal husbandry is promoted, the preparation method is simple, the cost is low, and the compound preparation is suitable for industrial production. The method is suitable for large-scale production and application.

The invention discloses a completely human neutralizing antibody for resisting abrus precatorius toxin and application of the completely human neutralizing antibody, and belongs to the field of biological medicine. The antibody comprises a specific immunoglobulin heavy chain variable region and a specific immunoglobulin light chain variable region, the amino acid sequence of the heavy chain variable region is a sequence as shown in SEQ ID NO: 1 or a sequence with homology of 70% or more with the heavy chain variable region, and the amino acid sequence of the light chain variable region is a sequence as shown in SEQ ID NO: 2 or a sequence with homology of 70% or more with the light chain variable region. The anti-abrus precatorius toxin full-human neutralizing antibody disclosed by the invention has high affinity activity, and can effectively neutralize the toxicity of abrus precatorius toxin in vivo and in vitro, so that the anti-abrus precatorius toxin full-human neutralizing antibody can be effectively applied to poisoning emergency treatment and treatment of abrus precatorius toxin.

The invention provides a construction method of an animal model of chronic low-dose cadmium exposure induced hepatic fibrosis, and provides a research basis for the reason of chronic cadmium exposure induced hepatic fibrosis and the drug efficacy analysis of the chronic cadmium exposure induced hepatic fibrosis. The method simulates a chronic low-dose cadmium exposure condition, is convenient to operate and stable, can help scientific researchers to visually observe the occurrence and development process of chronic liver injury and hepatic fibrosis induced by heavy metal exposure, and can provide a powerful basis for mechanism research of heavy metal poisoning. According to experimental results, in the body shape, living state, liver appearance and liver tissue pathological change process of a mouse, the mouse shows the mouse liver chronic injury and fibrosis change process under the chronic low-dose cadmium exposure environment, the direct relation between the cadmium exposure time and the liver fibrosis progress can be directly obtained, and the obtained animal model is stable. Researchers can create a disease process similar to human diseases through construction of the animal model in the future, the disease process can be quickly simulated, mass copying can be performed in a short time, the research period can be shortened, the animal model can be used for large-scale experimental research, and powerful support is provided for drug research and development and treatment strategies.

Provided herein are methods of treating cannabinoid-induced toxicity. Further provided herein are injectable compositions for treating acute cannabinoid overdose, poisoning, or acute cannabinoid-induced CNS depression. Further provided herein are methods of treating cannabinoid-induced toxicity in pediatric patients.

The invention discloses a method for determining a quaternary ammonium salt pesticide in blood by using liquid chromatography-mass spectrometry, which comprises the following steps of: drawing a whole blood (plasma and serum) matrix labeling standard working curve through liquid chromatography-mass spectrometry analysis; obtaining peak areas of quantitative ion peaks of target objects containing paraquat, aquacide, diguat, chlormequat and mepiperidine with different concentrations and peak areas of quantitative ion peaks of internal standard substances, and further drawing a standard working curve; and performing liquid chromatography-mass spectrometry analysis on the whole blood sample under the same condition to obtain the peak area of a target substance quantitative ion peak and the peak area of an internal standard substance quantitative ion peak in the whole blood test solution, and calculating the content of each quaternary ammonium salt pesticide according to the standard working curve. The method can be used for simultaneously detecting the content of five quaternary ammonium salt pesticides in the whole blood of the poisoned patient simply, conveniently and quickly, and has the characteristics of simplicity, accuracy, good repeatability and high sensitivity.

In alternative embodiments, provided are polyheterocyclic uncharged bisoxime antidotes, including polyheterocyclic uncharged bisoximes, and methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein. In alternative embodiments, provided are methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain; or, treating, ameliorating or protecting (preventing) an organophosphate toxicity or poisoning or toxic exposure, or for treating, ameliorating or protecting (preventing) organophosphate inhibition of an acetylcholinesterase (AChE) comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein.

Described herein are compounds that reactivate acetylcholinesterase and associated methods of treating organophosphate poisoning.

The present invention belongs to the field of detection and inspection technology, and provides a MXene material modified by nanosilver, and a preparation method and application thereof. The method comprises the following steps: mixing MAX material, lithium fluoride and hydrochloric acid solution for etching to obtain a multilayer MXene material; mixing the multilayer MXene material and dimethyl sulfoxide for suction filtration to obtain a MXene film; coating the silver nitrate solution on the surface of the MXene film for heating to obtain a MXene material modified by nanosilver. The MXene material modified by nanosilver prepared by the present invention realizes the rapid non-invasive (or low-invasive) diagnosis of paraquat poisoning patients using biological fluid samples such as blood and urine, the rapid detection of herbicides in fruits and vegetables, and the efficient detection of chemical drug additions in traditional Chinese veterinary medicines. The present invention can be widely used in the field of biological detection technology and has strong practicality.

The invention aims to provide a microbial feed additive for preventing zearalenone poisoning, which can obviously improve the reproductive performance and growth performance of livestock and poultry and enhance the immunity of animals. The composite microbial agent mainly comprises the following components in parts by weight: 1-5 parts of DL-alpha-tocopheryl acetate, 1-3 parts of superoxide dismutase (SOD), 5-10 parts of coated vitamin C and 82-93 parts of a bacillus subtilis microbial agent. 100 parts by weight of feed contains 0.005-0.01 part by weight of the feed additive disclosed by the invention. The embodiment 1 of the invention is preparation of bacillus subtilis for degrading ZEA, and the embodiment 2 of the invention is preparation of the microbial feed additive according to the content of the invention. The embodiment 3 is a detoxification experiment of the microbial feed additive on the ZEA in the young sow feed. An embodiment 3 verifies that the traditional Chinese medicine composition can well prevent the problems of reproductive disorder, low production capacity, reduced immunity and the like caused by ZEA poisoning of young sows.

The application discloses application of pyrroloquinoline quinone (PQQ) or a derivative thereof in preparation of a product for relieving or preventing blue-green algae toxin poisoning of yellow catfish, and belongs to the technical field of aquatic animal feed. The PQQ or the derivative thereof can be used as an aquatic feed additive or a medicine, and is added in an amount of 3-6 mg / kg of feed to feed yellow catfish, so as to effectively relieve pathological damage of the liver of the yellow catfish caused by blue-green algae toxin through various ways such as improving key enzyme activity of liver function, inhibiting liver oxidative stress, relieving inflammatory reaction and reducing structural damage of tissues. The PQQ is a new food raw material approved in China in 2022, has advantages of high safety, good water solubility, moderate cost, similar dosage to that of feed vitamins and the like, and provides a safe, economical and convenient new nutritional intervention strategy for blue-green algae toxin poisoning of the yellow catfish, and has a wide industrial application prospect.

The invention discloses a W / O / W type nano-emulsion loaded with cocaine esterase as well as a preparation method and application of the W / O / W type nano-emulsion. The nano-emulsion is prepared from cocaine esterase, a phosphate buffer solution, soybean phospholipid, vitamin E polyethylene glycol succinate, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and medium chain triglyceride. The preparation method comprises the following steps: preparing the inner water phase; preparing an oil phase; slowly injecting the internal water phase into the oil phase under shearing to obtain W / O type primary emulsion; slowly adding the primary emulsion into the external water phase under shearing, and performing high-speed shearing to obtain a secondary emulsion; and performing high-pressure homogenization on the secondary emulsion to obtain the final W / O / W type nano-emulsion. The cocaine esterase is combined with the nano-emulsion for the first time, and the nano-emulsion prepared by the method has the effects of improving the temperature stability of the cocaine esterase and prolonging the in-vivo half-life period, and can be applied to rescue or prevention of cocaine acute poisoning.

Extract of Saccharomyces Cerevisiae Yeast Cell Walls Rich in β-Glucans for the Prevention of the Effects of the Mycotoxin Deoxynivalenol (DON) The present invention relates to extracts of Saccharomyces cerevisiae yeast cell walls rich in β-glucans, and their use in the prevention of mycotoxicoses caused by the mycotoxin deoxynivalenol, in particular in the prevention of liver and intestinal damage, and the immunotoxic effects caused by this mycotoxin. (no figure)

The present invention discloses the application of trans-anethole in alleviating the toxicity of zearalenone in animals, belonging to the technical field of mycotoxin prevention and control. The present invention provides the application of trans-anethole in the preparation of a drug or feed for alleviating zearalenone poisoning in animals. The present invention has confirmed that trans-anethole can effectively alleviate the reproductive toxicity of zearalenone in piglets, improve the uterine organ index and ovarian organ index, ensure the health of piglets and the development of the reproductive system, and provide a new method for detoxifying zearalenone.

The present invention discloses a detection kit and a detection method for type B botulinum neurotoxin. The present invention can complete qualitative detection of various suspected cases of botulinum neurotoxin poisoning in a short time, provide evidence support for assisting doctors in diagnosis and timely treatment decisions, and is of great significance in social health and safety. At the same time, the detection method of the present invention can significantly improve the specificity of toxin proteins, avoid the time problems caused by animal experiments such as serotype identification, and can be applied to the research and development and production process of botulinum neurotoxin products. In addition, the present invention can perform quality control on the stability and shelf life of botulinum neurotoxin standards.

Products, pharmaceutical compositions, methods of use, and kits are provided for treating a patient suffering from botulinum neurotoxin intoxication. Featured are single-domain variable heavy-chain (VHH) polypeptides (antibodies) that target and neutralize the light chain (LC) protease domains of botulinum neurotoxin (BoNT), i.e., BoNT LC / A and BoNT LC / B, and that advantageously provide late and post-exposure therapy for botulism patients. The LC / A toxin-binding and LC / B toxin-binding VHH polypeptides, which can be recombinantly produced, specifically bind to the LCs of the botulinum neurotoxins to inhibit toxin activity and treat intoxication.

The invention discloses application of sulforaphane, and belongs to the field of medicine. Experiments with mouse AML-12 cells find that when the mouse AML-12 cells are jointly treated with sulforaphane and alpha-amatoxin, the inhibition effect of alpha-amatoxin on the activity of the AML-12 cells can be effectively relieved, the ROS level in the cells can be reduced, cell oxidative stress injury can be relieved, mitochondrial membrane potential reduction can be inhibited, and the mouse AML-12 cells can be treated with raphane and alpha-amatoxin. The sulforaphane can be used for relieving alpha-amatoxin induced mouse AML-12 cell mitochondrial injury, so that the sulforaphane can become a novel medicine for relieving alpha-amatoxin poisoning. The new application of the sulforaphane is found for the first time, and a new substance is provided for preparing the medicine for relieving alpha-amatoxin poisoning.

The invention discloses an application of artemether, artesunate or dihydroartemisinin in preparation of a medicine for preventing or treating aflatoxin B1 poisoning. Belongs to the technical field of medicine. The technical problem of detoxification of aflatoxin B1 is solved. The invention provides an application of artemether, artesunate or dihydroartemisinin in preparation of a medicine for treating aflatoxin B1 poisoning diseases. The body can be promoted to detoxify the AFB1.

The invention belongs to the field of biological medicine, and relates to application of a TRPV4 inhibitor in preparation of a medicine for preventing or treating diseases caused by trimethyltin chloride poisoning. The TRPV4 ion channel inhibitor can remarkably prolong the incubation period of epilepsy caused by trimethyltin chloride poisoning, relieve the severity of epileptic seizure, improve nerve injury and increase the survival rate. Therefore, a new drug target is provided for diagnosis of trimethyltin chloride poisoning and drug research and development, and a new strategy is provided for clinical treatment of trimethyltin chloride poisoning.

The invention relates to the technical field of medical monitoring, and discloses an intelligent early warning method and system for local anesthetic poisoning, and the early warning method comprises the steps: obtaining multi-modal data containing the structural features of a patient, physiological time sequence data and an initial ultrasonic image; based on the ultrasonic image, generating a guide signal representing a space risk and a depth space feature vector in parallel; adaptive attention weighting is carried out on the physiological time sequence data under the guidance of a guidance signal representing the spatial risk, and an adaptive time sequence feature vector is generated; and finally, fusing the structural features, the depth space features and the adaptive time sequence features, determining a poisoning risk level, and performing early warning. By fusing the multi-modal data and utilizing the space risk analyzed by the ultrasonic image to guide the analysis focus of the time series data, the method can capture and predict the weak physiological change of the toxic reaction, thereby overcoming the defects of strong subjectivity and response lagging of the traditional monitoring, and improving the timeliness, accuracy and objectivity of early warning.

Activated carbon compounds for the detoxification of intoxications, including methods of use thereof are provided. Many embodiments provide an approach to detoxifying individuals, including household pets (e.g., dogs and / or cats) from toxic substances. Many embodiments are directed to compositions including activated carbon compounds that can be used to detoxify toxins and other intoxications in an individual. Many embodiments may use activated carbon spheres for detoxification. Many embodiments of compositions can be administered orally. In numerous embodiments, the individual is a human and / or a non-human animal, such as a household pet, including dogs and / or cats.

The invention relates to a biomarker for evaluating progression and prognosis of paraquat poisoning and application of the biomarker. The biomarker is a urine metabolite Inosine. Urine non-target metabonomics analysis shows that the level of the Inosine is low in urine of a patient suffering from early paraquat poisoning, is gradually reduced along with aggravation of the illness state and is remarkably increased in follow-up visit of five years; the prediction efficiency is judged according to the area (AUC) below the working characteristic (ROC) curve of a subject, the Anosine has good efficiency (AUC = 0.806) in judging the progression of paraquat poisoning, has good efficiency (AUC = 0.816) in judging the prognosis aspect in 1-4 days at the early stage of poisoning, and has excellent efficiency (AUC = 0.961) in judging the prognosis aspect in 7-14 days at the progression stage of poisoning. Moreover, the efficiency of the Inosine in predicting the progress and prognosis of paraquat poisoning is better than that of blood routine indexes (such as the number of leukocytes, the percentage of neutrophils and the percentage of lymphocytes) reported in the literature. In conclusion, as the biomarker for prognosis and prognosis of paraquat poisoning, the Inosine shows a good clinical application prospect.

To provide novel analogs of cytisine, and their use in medicine, for example in the treatment of addiction, as well as improved synthetic methods for the preparation of analogs of cytisine.SOLUTION: Disclosed are novel analogs of cytisine, a process for their preparation, pharmaceutical compositions containing them, and their use in the prevention of or treatment of CNS disorders including addictive disorders. The chemical structure of the novel cytisine analogs of the present invention is illustrated in the following chemical formula.SELECTED DRAWING: None