309 results about "Toxication" patented technology

A composition is provided which contains calcium chelators, bisphosphonates, and / or citrate compounds and which may be used for treating and or reducing pathological calcifications, heavy metal poisoning, the growth of Nanobacterium Calcifying Nano-Particles and calcification-induced diseases in humans and animals. The method includes administering a therapeutic composition of calcium chelators, bisphosphonates, and / or citrate compounds which effectively inhibit or treat the development of calcifications in vivo. Typically, the administered composition includes about 0.1-10:1 parts by weight of calcium chelators, bisphosphonates, and / or citrate compounds.

Methods of treating autoimmune disorders, coronary artery disease, allergy symptoms, allograft rejection sepsis / toxic shock are disclosed. Some methods comprise administering one or more regulatory compositions to activate the T suppressor cells by increasing the acetylation level and / or protein level of FOXP3 in combination with a T suppressor stimulus and / or an antigen. Some methods comprise administering one or more regulatory compositions to activate the T suppressor cells by increasing the acetylation level and / or protein level of FOXP3. Some methods comprise administering soluble GITR or antibodies that bind to GITR ligand. Methods of treating cancer, infectious diseases, and immune deficiency are also disclosed as are vaccination methods. The methods comprise administering one or more regulatory compositions to inactivate the T suppressor cells by reducing the acetylation level and / or protein level of FOXP3. Improved vaccines and vaccination methods are disclosed. Methods of identifying compounds that are useful to modulate acetylation level and / or protein level of FOXP3 and treat diseases are disclosed.

An anti-intoxication composition and method of making and using are provided for treating and preventing drinker's remorse and other toxic effects of excessive alcohol consumption by humans. The anti-intoxication composition is an acidic mixture containing sweeteners, flavoring agents, food additives and a processed mixture of metallic salts, sulfuric acid, ammonium sulfate and water. When the anti-intoxication composition is ingested, it promotes the beneficial metabolism of alcohol, primarily ethanol, in the body. The beneficial metabolism results in the conversion of alcohol to amino acids and eliminates the toxic reactions of a hangover, including, but not limited to, a pounding headache, nausea, dry mouth, shaking hands, hypersensitivity to bright lights and sounds.

The invention relates to a new application of low molecular citrus pectin, in particular to the application of low molecular citrus pectin for treating and preventing lead and heavy metal poisoning in prepared medicines, foods and health care products. The low molecular citrus pectin is a compound prepared from soluble dietary fiber and galacturonic acid, the polysaccharide derivative is used as a metal ion absorbent (chelating agent), has absorption action on heavy metal ions, can remove heavy ions, such as Zn2+, Cu2+, Cd2+, Ag+, Cr3+, Ni2+, Hg2+, Pb2+ and the like. The low molecular citrus pectin is different from common chemical chelating agents; proved by animal and clinic experiments, lead, mercury and arsenic are discharged out of human bodies during toxin discharge, and the key level of calcium, magnesium, zinc or other minerals can not be influenced. The natural chelating function of the low molecular citrus pectin is a most ideal and successful solution for heavy metal pollution in the human body at present. Thus, the low molecular citrus pectin has the function of preventing various diseases and especially has special functions of clearing away harmful cells and discharging toxins in the human body.

Provided herein are methods and recombinant expression systems for the production of recombinant glycoproteins that have increased sialic acid content and contain predominantly alpha2-6 sialic acid linkages. Also provided herein are recombinant glycoproteins that have an increased in vivo circulatory half-life. One potential application of the glycoproteins described herein is for the treatment and prophylaxis of poisoning by neurotoxins.

The invention discloses a chemiluminescence sensing detection method for organophosphorus pesticide residues and application thereof. The method is characterized by comprising the following steps: by taking a chemiluminescent probe as an indicator, indicating the organophosphorus pesticides to inhibit the degree of reducing choline produced by acetylcholine chloride subjected to catalytic hydrolysis of acetylcholin esterase, coupling choline oxidase to catalyze choline to generate hydrogen peroxide, then oxidizing a luminescence probe to generate chemiluminiscence by virtue of hydrogen peroxide, carrying out a multi-step coupling reaction to realize sensing detection of the organophosphorus pesticide residues by utilizing a chemiluminescence apparatus. According to the sensing method, 96 samples can be detected within 20 minutes, and dichlorvos is taken as an example, the detection limit reaches 1nmol / L. The method is sensitive and rapid and can be used for blood sample monitoring of patients suffering from poisoning of foods, water samples and organophosphorus poisons.

The invention discloses a method for measuring blood concentration of paraquat. The method comprises the following steps of: (1) pretreating a sample, namely adding aqueous solution of an internal standard substance and a protein precipitation agent acetonitrile into a plasma sample, performing whirl mixing, centrifuging and sampling supernatant; (2) separating the sample, namely adopting a universal C18 liquid chromatographic column, and using an ion pairing agent in an acid mobile phase, wherein the acid mobile phase is mixed solution of 3mmol.L<-1> aqueous solution of sodium dodecyl sulfate, 0.2 percent of aqueous solution of trifluoroacetic acid, acetonitrile and water; and (3) detecting by using a diode array detector, namely detecting by using the diode array detector at a detectionwavelength of 250 to 260nm, measuring peak areas of the internal standard substance and the paraquat, and calculating the blood concentration of the paraquat through least square method linear regression. The plasma sample is easy and convenient to pretreat, the detection process is sensitive and rapid, toxic substances and the blood concentration thereof can be rapidly determined in actual application, and the method is high in clinical application value.

A neuroprotective composition for protecting neuronal cells against oxidative stress and methods for using and preparing the same. More particularly, the neuroprotective composition of the invention comprises a therapeutically effective amount of ceruloplasmin or a functional derivative thereof. The neuroprotective composition is characterized in that it protects neuronal cells from reactive oxygen species such as .O2- and .OH. In a preferred embodiment, the neuroprotective composition further comprises an antioxidant consisting of catalase or of an amphiphilic physiological antioxidative solution comprising a mixture of pyruvate, antioxidant, and lipid(s) such as fatty acids. The neuroprotective composition could be used for the treatment of brain trauma, brain or cerebrovascular ischemia, neurodegenerative diseases, poisoning of neuronal cells, the diminution of drugs side effects and for preservation of neuronal grafts.

The invention discloses a method and device for selecting an optimal emergency evacuation route for a hazardous chemical enterprise. A leakage condition of a hazardous chemical enterprise and a jointdistribution probability of a wind farm are received, a real leakage scene set is constructed to carry out leakage simulation, gas concentrations of all preset monitoring points in all leakage scenesare recorded in real time, and a possible leakage diffusion region is determined; whether the gas concentrations of all to-be-evacuated sites in different leakage scenes reach a preset alarm thresholdis determined and the leakage scenes that may trigger alarming are used as evacuation scene sets of the to-be-evacuated sites; evaluation routes are determined and the evaluation routes connecting random route outlet points at accumulated boundaries of all possible leakage diffusion regions are selected from the determined evaluation routes to form a standby evaluation route set; and personnel toxication risks of all evacuation scenes of the standby evaluation routes are calculated to obtained an accumulated individual toxication risk, traversing of the standby evaluation route set is carriedout, and an optimal evacuation route is obtained by using a minimum accumulative individual toxication risk as an optimization function. Therefore, the blindness of the evacuation route selection iseffectively eliminated and the success rate of evacuation is greatly improved.

The present invention relates to a combination of a Neotyphodium species endophyte or endophyte culture in a symbiotic association with a host grass which association does not cause symptoms of toxicosis in animals through exclusion of ergovaline but, due to the inclusion of alkaloids including agroclavine, setoclavine or isosetoclavine, retains the ability to resist abiotic stresses and protect the grass from pests.

The invention provides an anti-intoxication, alcohol-expelling and liver-protection preparation which has the functions of anti-intoxication, alcohol-expelling and auxiliary protection on alcoholic liver injury. The preparation consists of the following raw materials: 20-50 parts of vine tea, 5-15 parts of galangal, 10-40 parts of mung beans and 10-40 parts of poria cocos. The vine tea contains a great amount of flavonoid substances, and the dihydromyricetin in the vine tea has special effects in removing toxicity in alcohol, preventing alcoholic liver and fatty liver, inhibiting liver cell deteriorate, reducing the morbidity of liver cancer and protecting the liver. The galangal is acrid and hot in property, can enter the spleen and stomach channels, has the functions of eliminating cold to stop pain and warming the middle energy and stopping throwing and has a certain alcohol-expelling function, and moreover the flavonoid substance galangin in the galangal has a protection function on acute alcoholic liver injury. The poria cocos has the effects of clearing damp and promoting dieresis, tonifying spleen and quieting the heart, can promote the alcohol in bodies to discharge out along with urine, and is beneficial to sobering up. The mung beans have the effect of clearing away heat and toxic materials, can effectively relieve the symptoms of dizziness and unclear speaking after drinking alcohol, and furthermore has the pharmacologic action of protecting the liver and the kidney.

Compositions and methods for treatment of toxin poisoning are disclosed.

The invention discloses a medicine compound for curing hepatitis, which includes following ingredients and proportion: glycyrrhetic acid monoamine 1-200 shares, L-hydrochloric acid cysteine 1-200 shares, and one of sodium chloride, anhydrous sodium sulfite and ethylenediamine sodium acetic acid or the compound of then, the sodium chloride is 1-1000 shares, the anhydrous sodium sulfite is 1-200 shares, the ethylenediamine sodium acetic acid is 1-100 shares; it also discloses the medicine compound and the manufacturing method and the use, the medicine is compound solution, it can be applied to liver poisoning resisting, reduces the transaminase, recovers the functions of cell; it is mainly applied to cure the chronic hepatitis.

The invention relates to a benzimidazole zinc complex and a preparation method thereof. A chemical name of the benzimidazole zinc complex is dichloro-2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole zinc complex. The preparation method includes following steps: mixing 2-(carboxymethyl)benzoic acid with o-phenylenediamine, placing the mixture in phosphoric acid, performing a heating process at 180 DEG C for 3 hours to obtain a purple-green reactant, cooling the reactant to room temperature, adding distilled water, performing a neutralization reaction to obtain a yellow-green solid, performing a washing process through distilled water for three times, carrying out a filtering process, carrying out a recrystallization process through alcohol to obtain a yellow-green solid 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole, namely a ligand, dissolving ZnCl2 and the ligand 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole in a proper amount of a DMF solvent to obtain a mixture, stirring the mixture uniformly at room temperature, performing a volatilization process for seven days to obtain a slight yellow-green block-shaped complex monocrystal. The complex employs biological non-toxic element zinc as a metal ion, can be used for preparing antitumor drugs, can inhibit generation of esophagus cancer cells, and is free from remaining in human body and causing poisoning in human body due to metal accumulation. The complex is simple in the preparation method, is high in yield, is low in cost and is suitable for an industrial production.

The invention relates to a method for preparing a tetrodotoxin-degrading substance from a tetrodotoxin-producing bacteria fermentation broth, and a preparation and detection method of an extract liquid. The method comprises a method for artificially causing plasmid loss, a tetrodotoxin-degrading substance extraction method, and a tetrodotoxin degradation detection method. According to the invention, for a first time, the existence of a tetrodotoxin-degrading substance in aeromonas molluscorum is found, and an extraction method of the substance is established. The method has certain potential to be used in large-scale productions of the tetrodotoxin-degrading substance. The extract of the bacteria fermentation broth has a tetrodotoxin-degrading effect, and can be used for preparing medicines used for treating tetrodotoxin intoxication. Also, a raw material can be provided for related fields such as scientific research and pharmaceutical industries.

The invention relates to a lignin compound that could prevent yellow staphylococcal bacteria and B type hemolytic streptococcus, and the medicine combination of the compound. The invention of lignin Morgan dandehon herb acid A and Rufescidride has obviously effect restraining yellow staphylococcal and B type hemolytic streptococcus. It is also respected to prevent the diseases caused by them, like boil, blain, toxicity epidermis necrolysis, pneumonia, etc.

The invention relates to a method for enriching and separating a trace amount of polychlorinated biphenyls (PCBs). According to the method, a trace amount of the PCBs in an environmental sample are enriched by a modified solid-phase microextraction column by a combining a method that a trace amount of the PCBs are subjected to linkage separation by a double column gas chromatography-electron capture detector, and then are eluted and detected. Compared with the prior art, the method has the advantages that: a macromolecule fixed phase of the solid-phase microextraction column is modified, a solid-phase microextraction technology is combined with the gas chromatography-electron capture detector, sampling, extraction, concentration, sample separation, and detection are combined into a simpleprocess, automaton is easy to realized, the amount of the pre-enriched samples and the detection time are reduced, and the effect of enriching the PCBs is improved; the nature is determined by a double chromatographic column, and PCB monomers with different polarities and structures are separated; the method has the advantages of high analysis speed, low manufacturing cost, and high detection sensitivity and specificity and the like, and is easy to operate; instruments are portable; and the detection limit of the coplanar PCB monomers in water can reach 10<-8> to 10<-7> mol / L, and the field test of residues of toxication forms of six kinds of main PCBs can be realized.

The invention discloses application of oridonin powder aerosol in treatment of acute lung injury. The oridonin powder aerosol is prepared by an oridonin nano-drug delivery system, wherein the dosage form of the nano-drug delivery system is selected from lipidosome, nanoparticles, nanosuspension, nanoemulsion and micro-emulsion. The medicines in the oridonin powder aerosol are stable, easily enter the deep parts of lung tissues, are positioned in a targeted mode and are convenient to carry and use, and the treatment of acute lung injury is promoted. The oridonin powder aerosol is used for treating the acute lung injury caused by infection, shock, smoking, trauma, toxin poisoning, inhalation of irritant gases, radiation, high oxygen and low oxygen, wherein the inhaled irritant gases comprise phosgene, diphosgene, triphosgene, chlorine, nitric oxides, formaldehyde, dimethyl sulfate, hydrogen chloride, hydrogen bromide, hydrogen fluoride, ammonia, ozone and sulfur dioxide.

The invention provides salt containing carotene and iodine and a processing method thereof for solving the technical problems, so that people can get vitamin A conveniently. The salt containing the carotene and the iodine comprises the iodine, the salt and the carotene in a weight ratio of 20-60: 900,000-1,200,000: 1-500. The salt containing the carotene and the iodine is prepared by adding the carotene into the salt so as to provide a substance for people to take the carotene. The carotene, which is a precursor of the vitamin A, can be converted into the vitamin A according to the requirement of a human body under the catalysis of in vivo enzyme, without a poisoning phenomenon caused by the excessive intake of the vitamin A. The edible salt containing the carotene and the iodine is a new approach for supplementing the vitamin A.

A method for the assay of acetylcholinesterase in a sample of saliva is provided. The method can be used for the detection of poisoning caused by carbamate insecticide or organic phosphorus-based agricultural chemicals in a patient. A kit using the method of the invention is also provided.

The invention discloses activated carbon infusion solution, preparation method therefore and use thereof for the manufacture of drug for treating cancer. The activated carbon infusion solution contains pure activated carbon microparticles injection solution, activated carbon microparticles injection solution, activated carbon injection solution adsorbing catalyst and various injection solutions carrying drugs, all of which are for intravenous use. It characterizes that the surface area of material activated carbon used is greater than or equal to 400-10000 M2 / g or higher, heavy metal content is less than or equal to 0.1-10 ppm, other dissoluble metal ion content is less than or equal to 0.1-10 ppm, crozzle is less than 2%, total pore cubage is greater than 0.3-6 cm2 / g, 0.15% methylene blue adsorptive value is greater than 6-30, the diameter of the activated carbon microparticles contained in this activated carbon infusion solution are ranged from 35 μm to 2 nm, the diameters are mainly ranged from 6 μm to 3 μm are not more than 1%, the microparticles which greatest diameter is 35 μm to 6 μm have not more than 2×104 / mg activated carbon micropowder. When this activated carbon microparticles are intravenously injected into blood, they have good compatibility with tissues, have no toxicity or side effect, have no harmful stimulation, have no immunogenicity, they are safe and effective. They have incredible curative effect in treating cancer, blood vessels, atherosclerosis, coronary heart disease, cerebral thrombosis, infectious diseases, azotemia, acute organic and inorganic poison poisoning.

The invention relates to the field of film preparation, in particular to a preparation method of a surface hard protective film for a guide wire / tunnel needle used in peritoneal dialysis catheter insertion. In the method, by utilizing a magnetron sputtering or arc ion plating deposition device, high-purity Ti is selected as a target material and high-purity Ar and N<2> are taken as working gas, and a TiN or TiN+Ti single layer protective film and a TiN+Ti multilayer protective film are deposited on the surface of the guide wire / tunnel needle used in the peritoneal dialysis catheter insertion so as to improve wear resistance, resistance to high-temperature and high-pressure sterilization, resistance to corrosion caused by disinfectant, bacterial resistance and biocompatibility of the guidewire / tunnel needle. By utilizing the preparation method, problems of the guide wire / tunnel needle used in the peritoneal dialysis catheter insertion such as local surface fall-off and patient infection and toxication resulting from high-temperature and high-pressure sterilization and soakage in disinfectant for a plurality of times are effectively solved.

Provided herein is a means which is effective for botulism diseases and the prevention of the botulism diseases. Specifically provided is a plurality of human anti-botulinum toxin type-A antibodies having different epitopes from one another. Also specifically provided is a composition for neutralizing botulinum toxin type-A, which comprises a combination of two or more of the antibodies and which has a high neutralizing activity.

The invention discloses a safe broad-spectrum povidone-iodine preparation and a preparation method and application thereof. Each 1000g of the safe broad-spectrum povidone-iodine preparation is prepared from 10 to 15.5g of polyvinylpyrrolidone, 5.5 to 7.5g of refined iodine, 1.8 to 8.3g of potassium iodide, 0.01 to 3g of potassium iodate, 0.01 to 1.25g of a surfactant, 2 to 8g of a pH regulator andthe balance of water. A weight ratio of the polyvinylpyrrolidone to the effective iodine is (1.6-3.6): 1. The safe broad-spectrum povidone-iodine preparation with a proper dosage cannot cause poisoning and digestive tract injury after being orally taken, can be used for preventing and treating diseases of animals, reducing antibiotic abuse and improving human food safety, and is good in stability, not easy to decompose under the illumination condition and long in preservation time.

The invention discloses detoxication engineering bacteria, and a preparation method and application thereof. The invention provides a recombinant plasmid which is obtained by introducing coded genes of detoxication esterase and coded genes of hydrolase into an enzyme cutting site in plasmid pETDuet-1. The engineering bacteria of the invention can be obtained by introducing the recombinant plasmidinto host bacteria. The engineering bacteria comprise the coded genes of the detoxication esterase and the hydrolase and can realize inducible expression of the detoxication esterase and the hydrolase. Crude enzyme solution obtained by fermentation of the engineering bacteria can also be protected. The engineering bacteria and the crude enzyme solution thereof can simultaneously degrade organic acid esters such as malathion, parathion, high-efficiency cypermethrin, acetofenate and the like and organophosphorus pesticides, and can be used for detoxication of organophosphorus poisoned warm-blooded animals. The invention provides a new way for biological pollution treatment of toxic and harmful compounds such as pesticides remained in water sources, soil and the like by using natural resources of eukaryote and prokaryote.

A composite medicine in the form of particles for preventing and treating lead poisoning is prepared from wolfberry fruit, galangal fruit, roasted oyster shell and taurine. Its advantages are high curative effect and no toxic by-effect.

The present invention relates treatments of a toxin in a subject. The toxin at least partially effects its toxicity in the subject via binding to a target cell of the subject. The present invention provides for methods, combinations and pharmaceutical compositions for decreasing or neutralizing the effect of a toxin in a subject, using, inter alia, an effective amount of a nanoparticle comprising an inner core comprising a non-cellular material, and an outer surface comprising a cellular membrane derived from a source cell. Exemplary toxins include acetylcholinesterase (AChE) inhibitors such as organophosphate poisoning.

The invention provides Ivermectin mixed micelle injection for reducing poisoning incidence and local reaction probability during the killing of livestock parasites and a preparation method thereof. The micelle injection is prepared by taking Ivermectin as active ingredients and adopting a cholate / phosphatide mixed micelle system, so the problem that the Ivermectin is difficult to dissolve in water is solved effectively, the medicine-carrying dose of the micelle injection is improved greatly. The mixed micelle injection prepared by the method has a simple preparation process, and can reduce the probability of toxic and side reactions during the killing of the livestock parasites.

The invention discloses a cell-metabonomics-based difference metabolite metabolic pathway of rubusoside lipotoxicity resistance and a research method, relates to research methods of natural plant drugeffective component action mechanisms, and aims to solve the problem that an existing single pharmacology method cannot systematically and comprehensively evaluate the natural plant drug effective component action mechanisms. The method includes the steps of firstly, using ultrahigh performance liquid chromatography-tandem mass spectrum technology to detect and analyze palmitic acid induced rateinsulin oncocyte and rubusoside intervened intracellular metabolites; secondly, screening 12 potential lipotoxicity related difference metabolites, and identifying; thirdly, screening four metabolic pathways of the rubusoside lipotoxicity resistance and related enzymes and genes on the pathways, and analyzing. The method can comprehensively, efficiently and fast evaluate the rubusoside lipotoxicity resistance action mechanism from cell level in a bias-free manner, and a demonstrative basis is provided for natural plant drug effective component cell metabonomics and action mechanism illustration.

The invention discloses an IgY antibody for resisting botulinus toxin substrate peptide, which can be prepared by the following steps: preparing SNAP25 short peptide subA by a chemical synthesis method or a gene recombination expression method; collecting eggs by subA immune laying chicken and extracting and purifying yelk immunoglobulin by a biochemical method. The antibody has the characteristics of high specificity of combining substrate linear short peptide subA, but not combining integral substrate SNAP25, has the capability of especially identifying zymolytic SNAP25 of A botulinus toxin,can be used as a detection reagent to be applied to the detection of the A botulinus toxin and the activity analysis of neurovirulence or endopeptidase as well as the poisoning diagnosis of clinicalkreotoxism, and has good market prospect and social significance.