23 results about "Glucuronidase" patented technology

The invention discloses a beta-glucuronidase mutant and application thereof, and belongs to the technical field of genetic engineering and protein engineering modification. The beta-glucuronidase mutant disclosed by the invention has the following mutation on the basis of an amino acid sequence as shown in SEQ ID NO.3: T512S or T512A. The catalytic efficiency of the beta-glucuronidase mutant T512S is improved by 13.92 times compared with that of AtGUS (-3t), and the beta-glucuronidase mutant T512S shows remarkable industrial application potential.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

A glucuronidase activator including an active ingredient that is a Levilactobacillus brevis strain I-3141 deposited under accession number CECT 7480.

This invention provides an in vitro cultured bezoar, its preparation method, and its application, belonging to the field of traditional Chinese medicine preparation technology. The preparation method of this invention involves obtaining a bile-resistant, high-β-glucuronidase-active *Escherichia coli* strain through bile gradient acclimation, which is then combined with *Enterococcus faecalis* to form a functional bacterial community. This community is then cultured in vitro through a three-stage process of proliferation, transformation, and crystallization, followed by gradient calcium ion-induced crystallization and vacuum freeze-drying to obtain in vitro cultured bezoar. This invention aims to solve the problems of low conversion efficiency, uneven crystallization, insufficient content of effective components, and unstable product quality in existing technologies. The obtained in vitro cultured bezoar product has significantly higher bilirubin and conjugated bile acid contents than the standards of the *Chinese Pharmacopoeia*, and the process is stable, controllable, and suitable for industrialization.

The invention relates to Leclercia adcarboxsaca and an application thereof in degradation of corn straws, the Leclercia adcarboxsaca is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 32394. The strain can secrete AA2 family DyP-type peroxidase, GH1 / GH3 beta-glucosidase, PL8 oligogalacturonic acid lyase and GH88 unsaturated glucuronic acid hydrolase, and has the capability of synergistically degrading lignocellulose. The culture method comprises the steps of activation, seed solution preparation and fermentation. The obtained fermentation liquor is applied to the corn straw in proportion, so that the surface structure of the straw can be effectively destroyed, and the degradation rate is increased; meanwhile, soil nutrients are remarkably activated, the content of rapidly available phosphorus, rapidly available potassium, soluble organic carbon, alkali-hydrolyzable nitrogen and the like in the soil is greatly increased, and the synergistic interaction of efficient straw degradation and soil fertility improvement is achieved.

This invention discloses a lutein composition for relieving eye fatigue and its preparation method, belonging to the field of functional foods. The process involves: reacting phycocyanin and kelp polyphenols to obtain modified phycocyanin; reacting fucoidan and β-glucuronidase to obtain modified fucose; reacting fish collagen peptides and zinc chloride to obtain collagen peptide-zinc chelate; reacting lutein silica cage complex and modified phycocyanin to obtain a first complex; reacting the first complex and modified fucose to obtain a second complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil to obtain a lutein complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil, then adding trehalose to obtain the lutein complex; and finally mixing the lutein complex with maltodextrin to obtain the lutein composition.

There is provided a compound of formula (I) wherein the conjugate is a group capable of binding a BA and joining the BA to the remainder of the compound, the cleavable quantum comprises at least one site capable of being cleaved by a beta-glucuronidase, the spacer provides a distance between the conjugate and a payload, the payload being a cytotoxic agent, such as MMAE, and BA is a binding agent selected from the group consisting of a humanized antibody, a monoclonal antibody, a chimeric antibody or a human antibody or an antigen-binding fragment thereof. (I)

A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a β-D-glucuronidase indicator system, and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. The first sheet and second sheet are configured to retard passage of carbon dioxide therethrough. Methods of using the device are also provided.

The invention belongs to the technical field of biology, and discloses colorectal cancer marking microorganism beta-glucuronidase and application thereof. The invention provides a marker combination of beta-glucuronidase genes derived from different microorganisms. The marker combination is analyzed in a CRC (cyclic redundancy check) classification model and an adenoma classification model, and results show that the marker combination has good diagnostic ability. Beta-glucuronidase gene combinations based on different microorganisms can be used for detecting CRC subjects, screening and diagnosis of CRC in different stages are assisted, and an important basis is provided for individualized treatment strategy selection.

The invention discloses a method for detecting beta-glucuronidase to evaluate the ability of shrimps to resist a decapod iridovirus type 1, and belongs to the technical field of biological breeding of aquatic animals. The method comprises the following steps: detecting beta-glucuronidase in a sample by qPCR (quantitative polymerase chain reaction) to obtain a 2-delta delta Ct value of the beta-glucuronidase, and evaluating the anti-decapod iridovirus type 1 capability of the shrimps according to the 2-delta delta Ct value, when the 2-delta delta Ct is less than or equal to 0.15, the resistance is evaluated to be high, when the 2-delta delta Ct is more than 0.15 and less than 0.45, the resistance is evaluated to be medium, and when the 2-delta delta Ct is more than or equal to 0.45, the resistance is evaluated to be susceptible. And through experimental verification, the prediction accuracy of unknown sample verification is more than 86.7%, and the AUC area of the ROC curve is 0.8864. In conclusion, the invention provides a standardized and operable evaluation method for macrobrachium rosenbergii disease-resistant molecular breeding.

The invention provides a quality control product for detecting vaginal secretion and a preparation method of the quality control product. The quality control product comprises a negative quality control product and a positive quality control product; the positive quality control product comprises 20-200 mM of a second basic buffer solution, 1-5% of a second enzyme protective agent, 0-0.04% of lactic acid, 0-0.005% of hydrogen peroxide, 0.01-0.1 U / mL of a second leukocyte esterase item, 0.02-0.1 U / mL of sialidase, 0.05-0.5 U / mL of a second N-acetylglucosaminidase item, 50-200 U / mL of a second oxidase item, 2-40 U / mL of a second coagulase item, 50-200 U / mL of beta-glucuronidase, 5-20 U / mL of proline aminopeptidase, 1-5 U / mL of a second alkaline phosphatase item and 0.2-0.2. And 1% of bovine serum albumin.

The invention discloses beta-glucuronidase, a nucleotide sequence of a gene of the beta-glucuronidase and application of the beta-glucuronidase, the gene of the beta-glucuronidase and a protein sequence of the beta-glucuronidase are related to food-borne hazardous substances, and the beta-glucuronidase capable of efficiently and specifically activating cancerogens such as heterocyclic amine HAAs is constructed. The inhibitor designed aiming at the enzyme can reduce interference on intestinal effective microbial communities and human body GUS activity to the maximum extent, side effects are smaller, a research and development basis is provided for further high-throughput drug screening, computer-aided drug design and antibody drug development, and the inhibitor has a great application prospect.

The application discloses a kind of Penaeus vannamei beta-glucuronidase gene molecular markers, it includes molecular marker A, molecular marker B, molecular marker C, molecular marker D, molecular marker E, molecular marker F, molecular marker G, molecular marker H, molecular marker I, molecular marker J, molecular marker K, molecular marker L, molecular marker M, molecular marker N, molecular marker O and molecular marker P on the sequence of Penaeus vannamei beta-glucuronidase gene;The molecular marker is significantly related to the cold tolerance of Penaeus vannamei, and can be used as a functional marker for the cold tolerance of Penaeus vannamei, specifically, genomic DNA of the muscle tissue of the Penaeus vannamei to be tested is extracted as template DNA for PCR amplification and purification of the amplification product, then the product is sequenced to determine the genotype of the molecular marker, thereby selecting individuals with superior genotypes for Penaeus vannamei breeding, and further accurately and efficiently breeding Penaeus vannamei varieties with strong cold tolerance and stress resistance.

Compounds of formula (I) are provided, wherein Conjugator is a group capable of bonding a BA and joining the BA to the rest of the compound, Cleavable includes at least one site that can be cleaved by a β-glucuronidase enzyme, Spacer provides distance between Conjugator and Payload, Payload is a cytotoxic agent such as MMAE, and BA is a binding agent.

The present divisional application discloses a kind of chlorinated spirogenin derivatives, preparation method and application thereof, and belongs to the technical field of marine fungi active ingredient analysis.The chlorinated spirogenin derivative is the derivative with the hybrid skeleton of chlorogentisyl alcohol and gabosine, the present application utilizes the polarity difference of chlorogentisyl alcohol and gabosine derivative to obtain a kind of chlorinated spirogenin derivative with novel structure from marine fungi Epicoccum sorghinum A chlorinated spirogenin derivative with novel structure is isolated from the fermentation culture of GST-5, and through in vitro anti-beta-glucuronidase test, it is shown that the chlorinated spirogenin derivative provided by the present application has good beta-glucuronidase inhibitory activity and low cytotoxicity, and has development prospect in the preparation of anti-beta-glucuronidase drugs.

Disclosed is a fluorescent probe for glucose oxidase β The application discloses a kind of with big stokes shift The structure of the fluorescent probe QM-GLU is shown as formula I.The QM-GLU has almost no fluorescence at 560 nm when excited by a 450 nm wavelength, but can selectively undergo a fluorescence opening reaction with GLU.The fluorescence intensity at 560 nm gradually increases with the increase of GLU concentration, and other common interfering substances do not significantly interfere with the detection of GLU by QM-GLU and have no effect.Due to the characteristics of its AIE mechanism, and has a large stokes shift (110 nm), which greatly reduces the interference of system background fluorescence and improves the sensitivity of GLU activity detection.Therefore, QM-GLU is suitable for high selectivity and high sensitivity fluorescence detection of GLU.

The application discloses a method for evaluating electronic waste pollution exposure risk based on simple functions, which comprises the following steps: collecting urine of people in a potential exposure area of electronic waste pollution, taking supernatant after centrifugation of the urine sample, sequentially adding 1% formic acid, an internal standard solution and a beta-glucuronidase / arylsulfatase mixed solution, extracting and enriching after enzymolysis, taking supernatant for instrument analysis, obtaining the concentrations of 2,4,6-TriBP, 2,4,5-TriCP and 4-MonoBP in the urine, simultaneously determining the concentration of creatinine in the urine and performing correction; three judgment functions are constructed, and when the values of the three functions are all less than 0, it is determined that the people are exposed to electronic waste. Through the combination of feature importance analysis and decision boundary projection, geometric visualization and function explicitness of the decision logic are realized, and finally three simple functions for predicting the electronic waste pollution exposure risk are formed, which are convenient for practical application.

A compound of formula (I) is provided (wherein the formula is a conjugator which is a group capable of binding BA and connecting BA to the remainder of the compound, a cleavable group which comprises at least one site which can be cleaved by a β-glucuronidase enzyme, a spacer which provides the distance between the conjugator and the payload, the payload which is a cytotoxic agent, e.g., MMAE, and BA is selected from a humanized, monoclonal, chimeric, or human antibody or its antigen-binding fragment). JPEG2026521949000480.jpg25165

The invention belongs to the technical field of microorganisms, and discloses a fermentation method of plant endophytic fungi for high yield of beta-glucuronidase, which comprises the following steps: inducing endophytic fungi to produce enzyme; identifying a conversion product of the endophytic fungi; preparing a crude enzyme solution; and enzyme production conditions are optimized. The method comprises the following steps: taking Microsphaeropsiarundinis DX-SES3 as a test strain, and carrying out fermentation on the test strain to obtain the microsphaeropsiarundinis. Carrying out induced enzyme production on the strain in a liquid culture medium containing glycyrrhizic acid (GL) ammonium salt as a unique carbon source; the conversion product is detected and identified through TLC, UPLC and LC-MS, and it is proved that the strain can produce beta-glucuronidase under induction of GL ammonium salt, so that GL is directionally catalyzed to generate GAMG. And through a single factor optimization experiment, the optimal inducer addition time, carbon source and concentration, nitrogen source and concentration and inducer addition amount of enzyme production of the strain are determined, so that the yield of beta-glucuronidase is further improved, and high yield of beta-glucuronidase by using microorganisms is realized. A low-cost, environment-friendly and safe production way is provided for producing glycyrrhetinic acid monoglucuronide (GAMG) with high added value by microbial conversion of GL, and valuable data support is provided for development and utilization of plant endophytic fungi.

This invention discloses a tumor microenvironment-responsive enzyme prodrug conversion nanocomposite and its preparation method, belonging to the field of nanomedicine delivery technology. The nanocomposite comprises a core layer containing a β-glucuronidase H560N mutant, a targeting layer coupled with an anti-HER2 scFv, and a PEG protective layer modified with an MMP-2 sensitive peptide. The H560N mutation inhibits enzyme activity at neutral pH and restores it at acidic pH. After the nanocomposite is cleaved by MMP-2 to expose the targeting ligand, it is internalized by tumor cells. The acidic lysosomal environment activates the enzyme activity to convert the prodrug. The preparation method includes enzyme mutant expression and purification, maleimide-thiol coupling with scFv, and aqueous self-assembly to form the nanocomposite. This invention achieves precise enzyme prodrug therapy with dual spatiotemporal responses.

This invention discloses a loofah extract with antioxidant activity and its preparation method, belonging to the technical field of plant extracts and natural antioxidants. The method uses loofah leaves, loofah peel, or a combination thereof as raw materials. After pretreatment, crushing, and pulping, the materials undergo acidic hypoxia enzyme-inhibiting pretreatment to reduce the activity of polyphenol oxidase and / or peroxidase. Cell wall degrading enzymes are then added for multi-stage enzymatic hydrolysis to release bound polyphenols. Subsequently, an enzyme source with β-glucosidase, β-glucuronidase, and esterase activity is used to perform directional aglycone conversion of the bound polyphenols, converting bound flavonoids and / or bound phenolic acids into free flavonoid aglycones and / or free phenolic acids. Finally, the extract is obtained through alcohol-water extraction, macroporous resin enrichment, microencapsulation, and drying. The extract obtained by this invention has a high proportion of free active ingredients, good antioxidant activity, and good storage stability.

This divisional application discloses a class of gabosine derivatives, their preparation methods, and applications, belonging to the field of marine fungal active ingredient analysis technology. This invention utilizes the polarity difference of gabosine derivatives to extract and isolate a novel gabosine derivative from the fermentation culture of the marine fungus *Epicoccum sorghinum* GST-5. In vitro anti-β-glucuronidase assays show that the gabosine derivative provided by this invention has good β-glucuronidase inhibitory activity and low cytotoxicity, demonstrating promising development potential in the preparation of anti-β-glucuronidase drugs.

This invention discloses a β-glucuronidase and its enzymatic hydrolysis method for preparing baicalin and glucuronolactone, belonging to the field of genetic engineering technology. The amino acid sequence of this β-glucuronidase is obtained by mutation of the amino acid sequence shown in SEQ ID NO.1, specifically a mutation at amino acid position 296 and / or amino acid position 329 of the amino acid sequence shown in SEQ ID NO.1. The mutated β-glucuronidase mutant obtained by this invention exhibits higher enzyme activity and stronger hydrolytic activity towards the substrate baicalin, thereby effectively improving reaction efficiency and substrate conversion rate in the hydrolysis of baicalin to produce baicalin and glucuronic acid.