Disclosed are methods for delivering an
enzyme to a subject's brain or bone. The methods include administering a
hyaluronidase to the subject and administering the
enzyme to the subject. The
hyaluronidase and the
enzyme are administered to the subject under conditions effective to deliver the enzyme to the subject's brain or bone. Compositions and kits which include
hyaluronidase and an enzyme are also disclosed, as are methods for increasing blood-brain
barrier permeability in a subject. Also disclosed are methods, compositions, and kits for delivering genes or other
nucleic acid molecules to a subject's brain or bone, as well as methods, compositions, and kits for delivering enzymes to a subject's tissues. The methods, compositions, and kits are disclosed as being useful in treating or preventing a variety of
enzyme deficiency diseases, such as those affecting brain and / or bone, e.g., as Canavan's
disease,
Fabry disease, Gaucher's
disease, various forms of
mucopolysaccharidosis (e.g., Hurler'
s syndrome, Scheie syndrome, Hurler-Scheie syndrome, Sanfillippo A syndrome, Morquio A syndrome, Morquio B syndrome, etc.), Niemann-Pick
disease, Schindler disease, and Pompe disease.