7 results about "Acidic amino acids" patented technology

This application discloses an HIV antigen comprising the gp41 protein; said gp41 protein contains at least one amino acid residue selected from the following: T at position 85; K at position 98; a basic amino acid R or K at position 109; a neutral amino acid L or I at position 111; a basic amino acid K or R at position 132; N at position 133; and an acidic amino acid D or E at position 139. The HIV antigen of this application has the advantages of high specificity and high sensitivity.

The application discloses a construction method and application of an NLRP3 humanized mouse model, and belongs to the technical field of genetic engineering. The construction method of the NLRP3 humanized mouse model comprises the step of mutating the 708th amino acid of mouse Nlrp3-201 protein into a non-acidic amino acid; the Ensembl ID of the mouse Nlrp3-201 is ENSMUST00000079476.10. It is verified that the 708th amino acid of the mouse NLRP3 protein is mutated into a non-acidic amino acid (for example, alanine), the NLRP3 of the obtained mutant transgenic mouse is similar to human NLRP3, and cannot be cut by caspase-3, and humanization is realized. The mutant mouse model can better reflect the biological processes such as human inflammasome activation, cell pyroptosis and inflammatory reaction, and provides a more accurate animal model for understanding human diseases and drug screening.

This application discloses a nuclear localization signal AoNLS and its application in enhancing CRISPR / Cas9 editing in zebrafish. The nuclear localization signal AoNLS is derived from the zebrafish proteome. Through bioinformatics mining and functional screening, a structurally unique amino acid, KRKREAE, was identified, with its amino acid sequence shown in SEQ ID NO:1. This sequence is composed of both basic and acidic amino acids, and its mode of action differs from the single charge-driven mechanism of traditional NLS, achieving a breakthrough improvement in nuclear input efficiency. The Pcs2-AoNLS-GFP system, constructed by fusing AoNLS with GFP protein, showed a 1.56-fold increase in nuclear input efficiency in zebrafish embryos compared to the traditional SV40 NLS. Simultaneously, the Pcs2-AoNLS-Cas9 system, constructed by fusing AoNLS with Cas9 protein, significantly enhanced the editing efficiency of the Stat3 and Amh target genes by 1.72 times and 1.82 times, respectively, compared to the Pcs2-SV40 NLS-Cas9 gene editing system. The AoNLS sequence provided in this application is short and highly efficient, and can be used as a core component of the next-generation gene editing tool nuclear delivery system, with good application prospects in the fields of aquaculture breeding and developmental biology research.

The invention claimed herein relates to a dielectric polishing composition and method thereof. The invention claimed herein particularly relates to a composition comprising: (A) surface-modified colloidal silica particles comprising negatively charged groups on the surface of the particles, wherein the surface-modified colloidal silica particles have a negative charge, a particle size of 60 nm to 200 nm, and a zeta potential of < -35 mV at a pH in the range of > 2.0 to < 6.0; (B) at least one corrosion inhibitor selected from at least one guanidine derivative; (C) at least one iron (III) oxidizing agent; (D) at least one buffering agent selected from at least one acidic amino acid having an isoelectric point (pi) of < 5.0; (E) at least one stabilizer; and (F) an aqueous medium, wherein the pH of the composition is in the range of > 2.0 to < 4.3, and wherein the composition has a polyacrylamide content of < 1 ppm.

This invention discloses a synergistic water-soluble fertilizer composition of macroelements and its application, relating to the field of agricultural fertilizer technology. The composition includes the following components: macroelements comprising nitrogen, phosphorus pentoxide, and potassium oxide, with a mass ratio of 20:20:20; ammonium polyphosphate, potassium dihydrogen phosphate, sulfur-based raw materials, and chelated trace elements; L-type acidic amino acids; and soluble organic matter (DOM). This synergistic water-soluble fertilizer composition of macroelements and its application, by compounding ammonium polyphosphate and potassium dihydrogen phosphate at a mass ratio of 1:(0.1-10) and using L-type acidic amino acids as chelating carriers, allows the chelating ability of ammonium polyphosphate for calcium and magnesium ions and the transport capacity of L-type acidic amino acids for trace elements to synergistically superimpose, thereby effectively inhibiting the formation of insoluble phosphate precipitates by the combination of phosphorus with calcium and magnesium ions in hard water or calcareous soil environments.

This invention provides an amino-PRX (Amino-PRX) vector as a nucleic acid / protein carrier. Amino-PRX freely provides amino groups to the sgRNA and acidic amino acids of Cas9, and CDs rotate and move only by mixing Amino-PRX and Cas9RNP (automatic molecular imprinting), thereby efficiently and easily forming a complex with Cas9RNP and achieving efficient delivery of Cas9RNP into cells. Furthermore, by optimizing the structure of the axon / cap connector and the amino / CD connector, the dynamics of Cas9RNP within cells can be precisely controlled, improving genome editing efficiency.

The object of the present invention concerns a hair restructuring association comprising or alternatively consisting of: (a) a quaternary ammonium salt of formula (I) and (b) at least one sulpho-derivative of vegetable fatty acids, wherein R5, R6 are independently chosen between hydrogen and a radical R0, R0 consists of the following structure of formula (II), R1 is chosen from the group consisting of: hydrogen, methyl, isopropyl, sec-butyl, isobutyl, ethylenemethylthio, benzyl, para-hydroxybenzyl and 3-methylene-1H-indole, R2, R3, R4 are independently chosen from the group consisting of: methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl and tert-butyl, mX− is chosen from the group consisting of: formic acid, acetic acid, unsaturated monocarboxylic acids, adipic acid, aldaric acid, oxalic acid, phthalic acid, azelaic acid, sebacic acid, malonic acid, succinic acid, tartaric acid, glutaric acid, pimelic acid, maleic acid, malic acid, fumaric acid and suberic acid, isocitric acid, citric acid, fatty acids, acidic amino acids, keto acids and aromatic carboxylic acids, m is an integer number comprised between 1 and 22, n is comprised between 2 and 20.