43 results about "Acidic amino acids" patented technology

The invention provides a copolymer polypeptide as well as a pharmaceutical composition and application thereof. The copolymer polypeptide is formed by linear random copolymerization of a first monomer, a second monomer and a third monomer; the first monomer is selected from any one of alkaline amino acid and derivatives thereof; the second monomer is selected from any one of acidic amino acid and derivatives thereof; the third monomer is selected from any one of neutral amino acid and derivatives thereof. The copolymer polypeptide and the pharmaceutical composition thereof provided by the invention can effectively relieve hyperkeratosis caused by psoriasis, relieve inflammatory enteritis and effectively inhibit tumor growth.

The present invention provides an aminated polyrotaxane (PRX) (Amino-PRX) carrier as a nucleic acid / protein carrier. Amino-PRX allows for efficient intracellular Cas9 RNP delivery through efficient and simple formation of a polyplex with Cas9 RNP by freely providing amino groups to Cas 9 sgRNA and acidic amino acids, just mixing Amino-PRX and Cas9 RNP (automatic molecular imprinting), and causing rotation and movement of CDs. Further, structural optimization of the axial / endcap linker and the amino group / CD linker enabled strict control of intracellular dynamics of Cas9 RNP and high genome editing efficiency.

The invention relates to a liquid crystal emulsion composition for skin barrier care. The liquid crystal emulsion composition is characterized by at least containing a component (a) fatty acyl acidic amino acid fatty alcohol ester (general formula I) and a component (b) fatty acyl amino acid salt (general formula II) and / or a cosmetically acceptable medium or carrier. The composition can spontaneously form a lamellar liquid crystal structure in a system, and the arrangement mode of the lamellar liquid crystal structure is highly similar to that of intercellular lipid of a skin cuticle, so that the skin barrier is remarkably strengthened, the transdermal moisture loss is reduced, and a light, smooth and non-greasy skin feeling is brought. The formed liquid crystal structure keeps stable in a temperature range of-18 DEG C to 45 DEG C, and is suitable for various repair cream, emulsion and essence products.

The invention discloses a liquid crystal emulsion composition with a barrier effect. The composition comprises fatty acyl acidic amino acid fatty alcohol ester as shown in a general formula (1) and an alkyl phosphate emulsifier as shown in a general formula (2), a layered liquid crystal structure is formed through self-assembly, and skin cuticle lipid arrangement is simulated. The liquid crystal emulsion composition can enhance the physical barrier of skin, reduce water loss, and maintain the uniformity and stability of liquid crystals under the conditions of temperature change, shear action and long-term storage. Compared with the existing liquid crystal composition, the composition provided by the invention has higher liquid crystal structure stability and also has a barrier protection effect.

The invention discloses a preparation method and application of an environment-friendly CO2 enhanced embedding channeling sealing system, and relates to the technical field of embedding of carbon dioxide. The preparation method comprises the following steps: mixing inorganic silicate containing metasilicate, acidic amino acid containing carboxyl groups at two ends of a molecule, an anionic surfactant containing aromatic rings, an inorganic nano-material containing silicon hydroxyl, amino acid decarboxylase wrapped by gelatin and water, and uniformly stirring and mixing the required raw materials to form a mixed system; and controlling the temperature of the mixed system to be 50-70 DEG C, and reacting at the temperature to form the semi-solid silicic acid gel. The CO2 reinforced burying and channeling sealing system prepared by the method has the advantages of good injection performance, high temperature resistance, long temperature-resistant validity period and the like; and the system is low in viscosity before curing, controllable in curing time, high in curing strength and simple and convenient in site construction, and meets the requirements of CO2 oil displacement and long-term burying and channeling sealing.

The invention belongs to the field of enzyme engineering and agricultural waste conversion, and particularly relates to a preparation method and application of a keratinase mutant for efficiently degrading feather feed in an acidic gastric juice environment. The method comprises the following steps: replacing amino acid Arg247 with positive charges on the zymoprotein surface based on cotG-KERQ7 in an environment with the pH value of 4, then carrying out rational design on the chemical property of a KERQ7 oxygen negative ion cave, and directly introducing acidic amino acid, so that a mechanism that the serine protease oxygen negative ion cave actively dynamically adjusts the charge density through pH-dependent protonation is introduced, and the pH-dependent protonation of the serine protease oxygen negative ion cave is changed into the pH-dependent protonation of the serine protease oxygen negative ion cave. According to the method, the keratinase is used as a raw material, the oxygen anion hole adaptive low-pH environment mutant is obtained, the acid stability and activity of the keratinase mutant are both improved, an efficient mutant enzyme preparation with activity and acid resistance remarkably improved in the low-pH environment is finally obtained, reference is provided for subsequent improvement of the application value of the keratinase in the feed industry, and the method has a wide application prospect. Wide market application prospects are realized.

The application discloses a fluorescent probe for simultaneously detecting chiral acidic amino acids and basic amino acids, a preparation method, a detection method and a paper-based sensor thereof, and a specific chemical structural formula of the fluorescent probe is shown in the following formula: the fluorescent probe is simple in preparation and low in cost, and is high in detection sensitivity and good in selectivity for the acidic amino acids and the basic amino acids.

The invention relates to the technical field of biological medicine, in particular to a compound formula capsule for enhancing the heart protection function of NMN through small molecule peptide. The active ingredients of the capsule comprise NMN and specific small molecule peptides shown in SEQ NO 01-05, and the mass ratio of the NMN to the specific small molecule peptides shown in SEQ NO 01-05 is 7: 1.5: 1.5: 1.5: 1.5: 1.5. The five small molecule peptides respectively contain basic / acidic amino acids, Gly-Pro structures, disulfide bonds and other characteristic sequences and are prepared through solid-phase synthesis or recombinant expression, and the purity is greater than or equal to 95%. A capsule carrier is gelatin or plant cellulose, auxiliary materials comprise microcrystalline cellulose and magnesium stearate, a dry granulation process is adopted, particles with the particle size of 80-120 meshes are prepared, the filling amount of each particle is 0.5 g, and the disintegration rate in simulated intestinal juice within 1 hour is larger than or equal to 85%. The formula can form a uniform dispersion system, and is suitable for various dosage forms such as capsules, tablets and the like.

This application discloses an HIV antigen comprising the gp41 protein; said gp41 protein contains at least one amino acid residue selected from the following: T at position 85; K at position 98; a basic amino acid R or K at position 109; a neutral amino acid L or I at position 111; a basic amino acid K or R at position 132; N at position 133; and an acidic amino acid D or E at position 139. The HIV antigen of this application has the advantages of high specificity and high sensitivity.

The invention provides a spider silk protein modified body easy to purify as well as a modification method and a purification method thereof, a plurality of amino acid residues in flexible regions on two sides of a spider silk protein microcrystalline region are replaced with acidic amino acids or alkaline amino acids, so that the proportion of charged amino acids in the flexible regions is greater than or equal to 7%, and the spider silk protein modified body is obtained; the spider silk protein modified body is purified in the form of an inclusion body through a temperature change process of heating and cooling. The modified body can be simply purified with high recovery rate through simple heating and cooling processes, the total recovery rate is up to 98% or above, the purity is 90%-99% or above, and meanwhile, the formed artificial spider thread is endowed with higher tensile strength by strengthening the interaction of beta-sheet on the two sides of a microcrystal region, especially between different chains. A foundation is further laid for large-scale efficient production and low-cost separation and purification of the high-performance spider silk protein, and the application possibility of the spider silk protein in various fields is greatly enhanced.

The present invention relates to a preparation method of carbonization-modified steel slag with improved hydration activity, modified steel slag and an active admixture for cementitious materials. The preparation method of the present invention includes: dissolving acidic amino acids to obtain an amino acid solution and heating it to the reaction temperature; adding steel slag into the stirring amino acid solution to produce a steel slag suspension, and then introducing CO2 gas into the steel slag suspension to start the reaction; after the reaction is completed, separating the solid from the system, and adding ethanol to the obtained solid, and drying to obtain steel slag. The preparation method of the present invention breaks the inherent view of the prior art, realizes the simultaneous improvement of the soundness and hydration activity of steel slag, and even can improve the hydration activity by extending the reaction time. The operation of the present invention is simple and has strong practicability. In addition, the acidic amino acid additive used belongs to a bio-friendly material and is non-toxic and harmless.

The present invention provides a cyclic peptide represented by formula (I)[wherein A is selected from the ring-forming groups A1 to A5; Xaa1 is a residue of an aromatic amino acid; Xaa2 is a residue of an aromatic amino acid, a basic amino acid, or an aliphatic amino acid; Xaa3 and Xaa8 each have a structure independently selected from a residue of an amino acid represented by formula (III) or (III′) in which thiol groups of Xaa3 and Xaa8 form a bond; Xaa4 is a residue of a neutral amino acid; Xaa5 is a residue of a basic amino acid; Xaa6 is a residue of a neutral amino acid or an acidic amino acid; Xaa7 is a residue of an aromatic amino acid; Xaa9 is a residue of an aromatic amino acid, an aliphatic amino acid, or a basic amino acid; Xaa10 is a residue of an aromatic amino acid; and Xaa11 is a residue of an aliphatic amino acid], or a pharmaceutically acceptable salt thereof.

The present disclosure provides a peptide tag, and a nucleic acid encoding the peptide tag. The peptide tag of the present disclosure can reduce an aggregation property of a protein in a cell. Specifically, the peptide tag of the present disclosure can be a peptide tag in which 5% or more and less than 45% of amino acids contained in an amino acid sequence thereof are acidic amino acids, and (b) 20% or more of the amino acids contained in the amino acid sequence are amino acids selected from the group consisting of F, P, Y, G, S, Q, N, and A.

The application discloses a construction method and application of an NLRP3 humanized mouse model, and belongs to the technical field of genetic engineering. The construction method of the NLRP3 humanized mouse model comprises the step of mutating the 708th amino acid of mouse Nlrp3-201 protein into a non-acidic amino acid; the Ensembl ID of the mouse Nlrp3-201 is ENSMUST00000079476.10. It is verified that the 708th amino acid of the mouse NLRP3 protein is mutated into a non-acidic amino acid (for example, alanine), the NLRP3 of the obtained mutant transgenic mouse is similar to human NLRP3, and cannot be cut by caspase-3, and humanization is realized. The mutant mouse model can better reflect the biological processes such as human inflammasome activation, cell pyroptosis and inflammatory reaction, and provides a more accurate animal model for understanding human diseases and drug screening.

The invention provides acidic amino acid modified brewing arrowroot with low caking rate and a preparation method thereof, and relates to the technical field of food processing. The method provided by the invention comprises the following steps: infiltrating arrowroot powder in an acidic amino acid solution, and drying at 40-60 DEG C to obtain a compound; and carrying out heat treatment on the compound at 120-150 DEG C to obtain the brewing arrowroot powder. According to the invention, through acidic amino acid solution infiltration treatment, hydrogen ions, hydrogen bond donors and hydrogen bond receptors are provided for the system, and browning of arrowroot flour at high temperature is prevented; through low-temperature drying treatment, the radix puerariae powder is prevented from being gelatinized at high temperature, and the integrity of radix puerariae powder particles is guaranteed; through high-temperature complexing treatment, hydrogen bonds are formed between the arrowroot flour and amino acids, so that the solubility of the arrowroot flour is greatly improved, and the brewing caking rate of the arrowroot flour is reduced. Acidic amino acid solution infiltration, low-temperature drying and high-temperature complexing treatment are combined, under the condition that low-content acidic amino acid auxiliary materials are used, the natural particle shape of the arrowroot flour is kept, the brewing caking rate of the arrowroot flour is reduced, and the purity of the arrowroot flour is guaranteed.

The invention relates to a peptide for use in prophylaxis, prevention, inhibition and / or treatment of psoriasis of a mammal, wherein the peptide comprises amino acids joined by peptide bonds, and wherein the peptide comprises an amino acid sequence of from 3 to 5 amino acids comprising 2 acidic amino acids and proline (P) (Pro). The invention also relates to a pharmaceutical composition for use in prophylaxis, prevention, inhibition and / or treatment of psoriasis of a mammal, wherein the pharmaceutical composition comprises a peptide, wherein the peptide comprises amino acids joined by peptide bonds, and wherein the peptide comprises an amino acid sequence of from 3 to 5 amino acids comprising 2 acidic amino acids and proline (P) (Pro). The invention further relates to a composition for skin improvement, a cosmetic composition, a topical skin cream composition and a nutritional compositions, wherein said compositions comprise a peptide comprising amino acids joined by peptide bonds, wherein the peptide comprises an amino acid sequence of from 3 to 5 amino acids comprising 2 acidic amino acids and proline (P) (Pro).

This polypeptide is for improving the antigen-binding properties of an antigen-binding polypeptide in a cell. The polypeptide comprises 3 to 20 (inclusive) amino acid residues, wherein 57.0% to 100.0% (exclusive) of amino acid residues constituting the polypeptide comprises acidic amino acid residues. Also provided is a fusion polypeptide comprising the polypeptide and an antigen-binding polypeptide.

The present application provides an oil-in-water emulsified cosmetic, which comprises (A) a powder, (B) a water-soluble thickening agent, and (C) a surfactant, the content of the (A) component is 1-30% by mass based on the total amount of the cosmetic, the (A) component contains (A1) a powder treated with an N-acyl acidic amino acid, the (C) component contains (C1) a solid nonionic surfactant with an HLB of less than 7 and (C2) a surfactant with an HLB of 7 or more, and the mass ratio [(C1):(C2)] of the (C1) component to the (C2) component is 1.5:1-30:1.

The invention discloses an anti-cell caking agent and application thereof. The anti-cell caking agent comprises a serum-free culture medium, wherein the serum-free culture medium contains acidic amino acid with the final concentration of 1-50 mg / ml and totipotent nuclease with the final concentration of 1-5 v / v%. The acidic amino acid and the totipotent nuclease in the anti-cell-caking agent have no toxic effect on cells and can improve the activity of the cells, and the totipotent nuclease interacts with free nucleic acid in a culture medium and has no influence on nucleic acid in the cells, so that the overall safety in cell culture is high.

To provide a solid composition containing ibuprofen with improved dissolution properties. [Solution] A solid composition comprising (A) ibuprofen and (B) at least one selected from the group consisting of noscapine, dextromethorphan, and salts thereof (excluding (i) a solid composition that does not contain dextromethorphan or a salt thereof and in which ibuprofen and noscapine or a salt thereof exist as different granules, and (ii) a solid composition that does not contain noscapine or a salt thereof and in which ibuprofen, dextromethorphan or a salt thereof, a polymer that forms a hydrogel, and an organic acid or an acidic amino acid).

The embodiment of the invention relates to the technical field of targeted imaging molecules, and discloses a linker, a targeted ligand, a radiopharmaceutical and a preparation method and application thereof. Wherein the linker is composed of 2-3 amino acids, the amino acids at least comprise acidic amino acids, the acidic amino acids are selected from one or more of aspartic acid, glutamic acid and 3-sulfoalanine, and the linker is used for coupling a targeted pharmacophore and a radionuclide binding group to prepare a targeted ligand and a targeted radiopharmaceutical. The connexon has the characteristics of high hydrophilicity and high binding force with plasma protein by limiting the number of amino acids and selecting acidic amino acids, so that the tumor uptake and residence time are prolonged, and the imaging contrast ratio is further improved.

A method of treating a tissue exhibiting eosinophil-associated inflammation in a subject is disclosed. The method comprises administering to the subject a composition comprising a therapeutically effective amount of a polymer and a pharmaceutically acceptable excipient. The polymer is formed from an acidic amino acid, such as aspartic acid and / or glutamic acid, or a pharmaceutically acceptable salt thereof. The polymer has an average molecular weight of at least about 5 kDa. The polymer binds to one or more eosinophilic granulocyte proteins in tissue to neutralize the eosinophilic granulocyte proteins and treat eosinophilic granulocyte-associated inflammation.

The invention discloses a synchronous dyeing and perming method for improving tensile strength and styling durability of hair. The method comprises the following steps: preparing a mixed solution of mercaptoacetic acid and a compound containing a carboxylic acid group, adjusting the pH value to be alkaline, immersing a hair bundle into the mixed solution for soaking, taking out the hair bundle, and winding the hair bundle on a perming tool; preparing a mixed solution of tea polyphenol and ferrous sulfate, immersing the mixed solution into a hair bundle wound on the perm tool, and taking out the hair bundle after dyeing and perming. According to the invention, disulfide bonds of hair are opened by using low-concentration mercaptoacetic acid at normal temperature, the hair is treated by using a compound (acidic amino acid or small molecular hyaluronic acid and the like) containing more than two carboxyl groups at the same time, and then the hair is dyed by using a complex formed by tea polyphenol-Fe < 2 + >, so that not only can the blackness similar to that of a commercial blackening agent be obtained, but also the hair can be dyed by using tea polyphenol-Fe < 2 + >. The perming efficiency can reach the effect similar to that of a commercial perming agent, the mechanical property of hair is improved to a certain degree, and low-temperature hair dyeing and perming are conducted synchronously.

The invention relates to the technical field of waste paper pulp treatment, and discloses a method for improving the performance of waste paper pulp, which comprises the following steps: S1, paper pulp pretreatment: S2, paper pulp impurity removal: putting to-be-treated pulp into screening equipment, and collecting to obtain undersize pulp; s3, extruding and dispersing: putting the undersize material into an extruding machine; s4, ultrasonic treatment: readjusting the concentration of the extruded slurry, and then putting the slurry into an ultrasonic processor to obtain pretreated slurry; s5, preparing a compound liquid: mixing alkaline amino acid, acidic amino acid and neutral amino acid according to a mass ratio, adding ionized water, and stirring to obtain the amino acid compound liquid; s6, modification treatment: mixing and stirring the pretreated slurry subjected to ultrasonic treatment and the amino acid compound liquid in S5; and S7, washing and drying: washing the mixed pulp after the reaction in the step S6 with deionized water to obtain the high-performance paper pulp. The method has the following advantages and effects: through physical and chemical combination and compounding treatment of multiple amino acids, synchronous optimization of whiteness and strength is realized.

The invention relates to the technical field of polypeptide, in particular to a polypeptide fermentation process based on fish viscera enzymatic hydrolysate and application of the polypeptide fermentation process in feed, and the polypeptide fermentation process comprises the following steps: (1) pretreating fish viscera; (2) carrying out composite enzymolysis; (3) membrane separation refining; (4) polypeptide fermentation; (5) fermentation product treatment; the fish viscera protein is degraded into 500-2000Da functional polypeptide by adopting a compound enzyme system formed by alkaline protease and papain according to a specific ratio and utilizing the synergistic effect that the alkaline protease cuts hydrophobic amino acid residue peptide bonds and the papain specifically hydrolyzes acidic amino acid residues; compared with single enzymolysis of the polypeptide, the yield is obviously improved. Meanwhile, the optimal action condition of the mimic enzyme is controlled by matching with stepped temperature, enzyme activity attenuation at single temperature is avoided, and the protein recovery rate is further improved.

The invention relates to a spider silk protein membrane material capable of generating electricity by friction and a preparation method and application thereof, a plurality of amino acid residues in flexible regions on two sides of a spider silk protein microcrystalline region are replaced with acidic amino acids or basic amino acids, and spider silk protein modifiers with different flexible region acidic / basic amino acid molar ratios are obtained; the spider silk protein modified body is independently formed or mixed with a conductive material to form a membrane, and the membrane material is formed. By utilizing the method disclosed by the invention, materials needing different piezoelectric properties can be developed, and a foundation is further laid for the industrial production of the recombinant spider silk protein and the wide application of the recombinant spider silk protein in the field of functional materials.

The invention relates to the technical field of adsorbents, in particular to modified sodium alginate as well as a preparation method and application thereof, and the modified sodium alginate is prepared from the following raw materials: sodium alginate, a cross-linking agent, a buffering agent, an activating agent and acidic amino acid. The modified sodium alginate provided by the invention can effectively adsorb and remove methylene blue in water, and can maintain good adsorption performance after multiple cyclic adsorption-desorption.

The present invention discloses a preparation method of glutathione (GSH)-modified silver nanoplates (AgNTs) and its application in detecting acidic amino acids; the present invention ingeniously overcomes the defect that GSH as an etchant for AgNTs will cause the transformation of AgNTs from triangles to discs, and proposes a post-modification technique, that is, by controlling the concentration of GSH, GSH is stably modified on AgNTs, thereby obtaining GSH-modified AgNTs; this technique can not only modify sufficient GSH on the surface of AgNTs, but also maintain the good triangular morphology of AgNTs; furthermore, the electrostatic attraction between GSH on the surface of AgNTs and acidic amino acids can cause the aggregation of AgNTs, and the degree of aggregation is proportional to the concentration of acidic amino acids, so as to achieve the purpose of detecting acidic amino acids.

This application discloses a nuclear localization signal AoNLS and its application in enhancing CRISPR / Cas9 editing in zebrafish. The nuclear localization signal AoNLS is derived from the zebrafish proteome. Through bioinformatics mining and functional screening, a structurally unique amino acid, KRKREAE, was identified, with its amino acid sequence shown in SEQ ID NO:1. This sequence is composed of both basic and acidic amino acids, and its mode of action differs from the single charge-driven mechanism of traditional NLS, achieving a breakthrough improvement in nuclear input efficiency. The Pcs2-AoNLS-GFP system, constructed by fusing AoNLS with GFP protein, showed a 1.56-fold increase in nuclear input efficiency in zebrafish embryos compared to the traditional SV40 NLS. Simultaneously, the Pcs2-AoNLS-Cas9 system, constructed by fusing AoNLS with Cas9 protein, significantly enhanced the editing efficiency of the Stat3 and Amh target genes by 1.72 times and 1.82 times, respectively, compared to the Pcs2-SV40 NLS-Cas9 gene editing system. The AoNLS sequence provided in this application is short and highly efficient, and can be used as a core component of the next-generation gene editing tool nuclear delivery system, with good application prospects in the fields of aquaculture breeding and developmental biology research.

The invention claimed herein relates to a dielectric polishing composition and method thereof. The invention claimed herein particularly relates to a composition comprising: (A) surface-modified colloidal silica particles comprising negatively charged groups on the surface of the particles, wherein the surface-modified colloidal silica particles have a negative charge, a particle size of 60 nm to 200 nm, and a zeta potential of < -35 mV at a pH in the range of > 2.0 to < 6.0; (B) at least one corrosion inhibitor selected from at least one guanidine derivative; (C) at least one iron (III) oxidizing agent; (D) at least one buffering agent selected from at least one acidic amino acid having an isoelectric point (pi) of < 5.0; (E) at least one stabilizer; and (F) an aqueous medium, wherein the pH of the composition is in the range of > 2.0 to < 4.3, and wherein the composition has a polyacrylamide content of < 1 ppm.