The present invention relates to a two-part
linker comprising
a peptide tag (
peptide) and a polypeptide (
protein) that is capable of spontaneously forming an isopeptide bond, particularly wherein: a)said
peptide comprises an
amino acid sequence as set forth in SEQ ID NO: 1, wherein: (i) X at position 1 is
arginine or no
amino acid; (ii) X at position 2 is
glycine or no
amino acid; (iii) X at position 5 is
histidine or
threonine; (iv) X at position 11 is
alanine,
glycine or
valine; and (v) X at position 14 is
arginine or
lysine, wherein when X at position 1 is no amino acid, X at position 2 isno amino acid; and b) said polypeptide comprises: i) an amino acid sequence as set forth in SEQ ID NO: 2; ii) a portion of (i) comprising an amino acid sequence as set forth in SEQ ID NO: 101; iii) an amino acid sequence with at least 80%
sequence identity to a sequence as set forth in SEQ ID NO: 2, wherein said amino acid sequence comprises a
lysine at position 34, a
glutamic acid at position 80and one or more of the following: 1)
threonine at position 5; 2)
proline at position 16; 3)
arginine at position 40; 4)
histidine at position 65; 5)
proline at position 92; 6)
aspartic acid at position 100: 7)
glutamic acid at position 108; and 8)
threonine at position 116, wherein the specified amino acid residues are at positions equivalent to the positions in SEQ ID NO: 2; or iv) a portion of(iii) comprising an amino acid sequence with at least 80%
sequence identity to a sequence as set forth in SEQ ID NO: 101, wherein the amino acid sequence comprises a
lysine at position 10, a glutamicacid at position 56 and one or more of the following: 1) arginine at position 16; 2)
histidine at position 41; 3)
proline at position 68; and 4)
aspartic acid at position 76, wherein the specified amino acid residues are at positions equivalent to the positions in SEQ ID NO: 101, and wherein said
peptide and polypeptide are capable of spontaneously forming an isopeptide bond between the
aspartic acid residue at position 10 of SEQ ID NO: 1 and the
lysine residue at position 34 of SEQ ID NO: 2 or position 10 of SEQ ID NO: 101.