65 results about "Peg modification" patented technology

The invention provides a nano-drug based on atomic layer deposition and a preparation method of the nano-drug, mesoporous silica is taken as a carrier, bimetallic nanoparticles are precisely deposited through ALD, and chemotherapeutic drugs and antibacterial components are loaded by combining amination and targeted PEG modification, so that the following functions are realized: (1) H2O2 in a tumor microenvironment is catalyzed to generate reactive oxygen species (ROS); removing fusobacterium nucleatum and damaging a biological membrane of the fusobacterium nucleatum; (2) GSH is consumed through Fenton reaction, and tumor drug resistance is reversed; and (3) the drug is released in pH response, and tumor cells are accurately killed. The invention further provides application of the nano-drug based on atomic layer deposition in treatment of related drug-resistant colorectal cancer induced by fusobacterium nucleatum, the process is controllable, the biocompatibility is excellent, the treatment effect on the drug-resistant colorectal cancer is remarkably improved, and the nano-drug has wide clinical application prospects.

This invention relates to the field of acrylic resin technology and discloses a stain-resistant polyethylene glycol-graphene-grafted acrylic resin coating. TEMPO-modified graphene is used as a polymerization carrier, and TEMPO free radicals serve as initiation sites to initiate in-situ chemical grafting polymerization of monomers such as n-butyl acrylate and acrylic acid on the graphene surface, resulting in a polyethylene glycol-graphene-grafted acrylic resin. The graphene is linked to the acrylic resin through polyethylene glycol molecular chains, organically combining the graphene and acrylic resin. This improves the dispersibility of graphene, overcomes its agglomeration in the acrylic resin, and significantly enhances the tensile strength and other mechanical properties of the acrylic resin. Simultaneously, the grafted polar polyethylene glycol molecular chains improve the hydrophilicity and stain resistance of the acrylic resin.

The invention discloses a polyethylene glycol modified gold and manganese dioxide nano particle and a preparation method and application thereof, and belongs to the technical field of biological medicine, the polyethylene glycol modified gold and manganese dioxide nano particle (GMCN and PEG) has good biocompatibility under neutral physiological conditions, can relieve tumor hypoxia and increase generation of active oxygen in acidic TME, and has a good anti-tumor effect. The radiotherapy sensitivity is improved; and immune cell death is induced. Meanwhile, a cGAS-STING signal channel can be activated, dendritic cell maturation, macrophage M1 polarization and T cell infiltration are promoted, and the immunosuppression state in TME is counteracted. In addition, the GMCN-coated PEG also has an MR-CT bimodal imaging enhancement function, and integration of diagnosis and treatment is realized. The nano sensitizer disclosed by the invention has huge potential in the aspects of improving the radiotherapy curative effect, remodeling the tumor microenvironment, promoting the anti-tumor immunity, enhancing the biomedical imaging and the like.

The invention discloses an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health. The enzyme-containing anti-inflammatory composition comprises an enzyme preparation, a hair follicle maintenance agent, a functional auxiliary agent and a carrier raw material. The core innovation lies in that a hair follicle targeted enzymolysis technology is adopted, and a specific enzyme modified by polyethylene glycol can efficiently dredge hair follicles; the anti-inflammatory and hair follicle repairing dual effects are achieved through anti-inflammatory-maintenance synergistic matching and adding sequence optimization; the damaged barrier is rapidly repaired by combining a composite barrier repairing agent and a gradient permeation process; enzyme activity and mildness are guaranteed by adopting a microcapsule embedding and low-temperature preparation technology. The composition can specifically solve the problems of hair follicle blockage, inflammation and unhairing, is suitable for pets of all ages and skin types, and is high in practicability.

The application relates to a carrier-free self-driven nanomotor and a preparation method and application thereof, and belongs to the technical field of biological medicines. The nanomotor is a nanometer assembly formed by co-assembly of a photothermal / light motion dual-enhanced photosensitizer and a near-infrared light response type nitric oxide donor BNN6 through intermolecular force, or is a nanometer assembly formed by co-assembly of a photothermal / light motion dual-enhanced photosensitizer and a near-infrared light response type nitric oxide donor BNN6 through intermolecular force and modified by a polyethylene glycol modifier. The application realizes a synergistic treatment mode of deep tumor penetration and high-efficiency killing by constructing a functional coupling system of "photothermal driving-NO release-motion enhancement-RNS cascade generation".

The invention discloses a nano preparation for natural product synergistic chemotherapy as well as a preparation method and application of the nano preparation, nanoparticles are formed by self-assembly of an SN38 prodrug sensitive to redox and galangin in a carrier-free manner, and can also be co-assembled nanoparticles modified by polyethylene glycol or entrapped with hydrophobic fluorescent molecules. According to the SN38 prodrug, a fluorene methanol side chain is coupled to an SN38 molecule through a redox-sensitive disulfide bond, so that a prodrug structure with a responsive release characteristic is constructed. The construction of the nano system not only significantly reduces the off-target toxicity of SN38 and improves the tolerance dose of SN38, but also enhances the SN38-mediated chemotherapy effect by the sensibilization effect of galangin, significantly improves the treatment efficiency, and realizes high-efficiency and low-toxicity cancer treatment. The invention provides a novel and synergistic prodrug nano drug delivery platform, and a new strategy is provided for precise treatment of refractory tumors.

The present invention discloses a PEGylated asparaginase and its application in drug preparation and clinical treatment. In the PEG-modified asparaginase of the present invention, one molecule of asparaginase is coupled with 13-45 molecules of polyethylene glycol, wherein the polyethylene glycol is a linear polyethylene glycol with an average molecular weight of 2-20 kDa. The asparaginase after PEG modification prepared by the present invention. The PEGylated asparaginase provided by the present invention has the advantages of reducing immunogenicity and significantly extending half-life, and the coupling of polyethylene glycol and asparaginase is more stable. Compared with the original research drug and generic drug products of the PEGylated asparaginase sold on the market, the PEGylated asparaginase provided by the present invention has a more stable structure, a firm PEG bond, is not easy to fall off, and has higher homogeneity and activity.

The present invention relates to nanoparticles of a metal chosen from among platinum, bismuth or a mixture thereof, which are functionalised at their surface by functionalised polyethylene glycol, comprising in particular at least one OH, COOH, NH2 or SH functional group, and to their method of preparation, which comprises of the following steps:a) Mixing a precursor of nanoparticles with a functionalised polyethylene glycol, in particular comprising at least one OH, COOH, NH2 or SH functional group, in water;b) Exposing the mixture to ionising radiation.

The invention discloses a hydroxyl-alpha-sanshool nano preparation as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations, the hydroxyl-alpha-sanshool nano preparation is HAS-coated PEG-PLGA, the preparation method comprises the following steps: taking hydroxyl-alpha-sanshool and polyethylene glycol modified polylactic acid-glycolic acid copolymer as raw materials, and adding the hydroxyl-alpha-sanshool nano preparation and the polyethylene glycol modified polylactic acid-glycolic acid copolymer to prepare the hydroxyl-alpha-sanshool nano preparation. The preparation method comprises the following steps: preparing HAS-coated TGN-PEG-PLGA by adopting an emulsified solvent evaporation method, and further modifying by adopting TGN polypeptide, so as to prepare HAS-coated TGN-PEG-PLGA; according to the hydroxyl-alpha-sanshool nano-preparation, the encapsulation efficiency and the drug loading capacity of hydroxyl-alpha-sanshool are obviously improved, and the hydroxyl-alpha-sanshool nano-preparation has excellent stability and blood-brain barrier crossing capacity, so that the hydroxyl-alpha-sanshool nano-preparation can give full play to the treatment effects of improving learning and memory ability, cognitive ability and the like.

An object of the present invention is to provide a lipid particle that can be produced without using a PEG-modified lipid, or that exhibits higher drug effects, safety, or stability than those of a lipid particle produced using a PEG-modified lipid; or that has immunokinetics different from those of a lipid particle produced using a PEG-modified lipid. A lipid particle comprising an ionized lipid, a phospholipid, and a sterol as lipid components of the lipid particle, and a modified polysaccharide containing a hydrophobic group.

The invention discloses a preparation method of reusable polyethylene glycol modified magnetic beads and application of the reusable polyethylene glycol modified magnetic beads in exosome extraction.The preparation method comprises the steps that EDC is added into carboxylated magnetic graphene oxide for a reaction, then NHS is added for a reaction, and a mixed solution is obtained; and adding an aqueous solution of aminated polyethylene glycol into the mixed solution, carrying out amidation reaction, carrying out magnetic suction washing with pure water, and drying. When the exosome is extracted by adopting the method disclosed by the invention, the exosome of 30-150nm can be obtained; the polyethylene glycol modified magnetic beads used in the invention have the advantages of low cost, fast preparation and strong magnetism, and can be completely separated from the solution within 30 s. The exosome is captured through a precipitation method, the activity of the exosome is not damaged after the exosome is released, the yield and the purity of the exosome are improved, the recovered magnetic beads can be repeatedly used, and the cost is reduced.

The present invention provides polyethylene glycol-modified polylysine, its preparation method, a nanovaccine, and its application. The polyethylene glycol-modified polylysine comprises a dendritic polymer initiator and chiral polylysine linked by amide bonds. By grafting polyethylene glycol onto the side chains of polylysine, the present invention modulates charge density and enhances nanoparticle migration to lymph nodes. Finally, the nanovaccine is prepared by binding to antigens through electrostatic adsorption. Research has shown that the resulting antigen-complexed nanovaccine promotes endocytosis by antigen-presenting cells (APCs) and their migration to lymph nodes, activating immune cells there. It can stimulate dendritic cells (DCs) to mature and release inflammatory cytokines. It also promotes antigen cross-presentation by DCs in vivo, triggering a strong antigen-specific immune response.

This invention relates to a polyethylene glycol-modified PrRP31, its preparation, and its application. The invention involves modifying PrRP31 with polyethylene glycol and testing its anti-inflammatory activity in a mouse paw edema model and its analgesic activity in a mouse water bath tail-flick experiment. Using polyethylene glycol-modified PrRP31, a mouse paw edema model, and a water bath tail-flick experiment, the anti-inflammatory and analgesic activities of polyethylene glycol-modified PrRP31 were tested. The experiments demonstrated that polyethylene glycol-modified PrRP31 possesses good anti-inflammatory and analgesic activity. In the mouse paw edema model, polyethylene glycol-modified PrRP31 inhibited inflammation by 37.09% (5 h) at a concentration of 5 mg / kg. In the mouse water bath tail-flick experiment, polyethylene glycol-modified PrRP31 produced the maximum analgesic effect at a concentration of 5 mg / kg. This invention provides an application of polyethylene glycol-modified PrRP31 as an anti-inflammatory and analgesic drug, offering beneficial assistance in the treatment of related diseases and possessing significant importance in the pharmaceutical field.

Provided are lipid nanoparticles showing excellent drug migration to the pancreas.The lipid nanoparticles according to the present invention are lipid nanoparticles for delivering a drug to a target tissue, comprising: (a) a phosphatidylcholine-type phospholipid having an unsaturated fatty acid chain with 16 to 24 carbon atoms, and (b) a polyethylene glycol-modified lipid, wherein the target tissue is the pancreas.

Provided is a method for preparing a polyethylene glycol-modified urate oxidase, at least 11 of the following amino acid sites of the polyethylene glycol-modified urate oxidase have a PEG modification: T1, K3, K4, K30, K35, K76, K79, K97, K112, K116, K120, K152, K179, K222, K231, K266, K272, K285, K291, and K293, and the preparation method includes: performing a coupling reaction between urate oxidase and polyethylene glycol, wherein the polyethylene glycol is provided in the form of an acidic solution, and a molar ratio of the urate oxidase to the polyethylene glycol is 1: (56 to 94), to obtain the polyethylene glycol-modified urate oxidase.

The invention relates to an adhesive for quickly curing a tea seed cake liquid mulching film into a film, which is characterized by comprising the following components in percentage by weight: 30-50% of tea seed cake extract; 5 to 15 percent of beet polysaccharide; 0.3 to 0.8 percent of nano SiO2; 0.05 to 0.2 percent of zinc humate; the adhesive comprises the following components in percentage by weight: 20-30% of beet polysaccharide, 10-20% of tea polyphenol, 10-20% of nano SiO2, 10-20% of nano SiO2 and 45-65% of water, the molar ratio of beet polysaccharide to tea polyphenol is 1: (1.5-2.2) so as to promote dynamic ester bond crosslinking, the nano SiO2 is modified by polyethylene glycol (PEG-4000), the zeta potential ranges from-25 mV to-35 mV, the nano SiO2 is used for regulating and controlling the surface tension gradient of a liquid film, and the adhesive can keep the dynamic crosslinking capacity for 72-80 hours when the pH value of soil ranges from 5.5 to 7.8. According to the adhesive for rapidly curing and forming the tea seed cake liquid mulching film, rapid curing for 35 + / -5 minutes is achieved through the catalysis of the zinc humate and the synergistic effect of the nano SiO2, compared with a traditional starch-based mulching film, the efficiency is improved, the farming operation interval is greatly shortened, a gradient pore structure is formed through induction of the nano SiO2, the surface layer is 5 microns / the bottom layer is 20 microns, wind erosion prevention and air permeability are both considered, the self-organization film forming thickness is 1.2 + / -0.3 mm, and the film forming efficiency is greatly improved. The coefficient of variation is less than 8%, and the film-forming property is excellent.

The application provides an organic phosphorus poisoning relief composite enzyme and application thereof, and belongs to the technical field of biological medicine. The organic phosphorus poisoning relief composite enzyme provided by the application comprises polyethylene glycol modified organic phosphorus hydrolase and butyrylcholine esterase, and the mass ratio of the polyethylene glycol modified organic phosphorus hydrolase to the butyrylcholine esterase is (4-8):(2500-10000). The rat acute organic phosphorus poisoning treatment result shows that the organic phosphorus poisoning relief composite enzyme provided by the application fully plays the efficient catalytic hydrolysis capacity of the enzyme as a catalyst, efficiently removes organic phosphorus and acetylcholine by using the organic phosphorus hydrolase and the butyrylcholine esterase respectively, provides a drug combination for treating the cause for the organic phosphorus poisoning injury first aid, and the treatment effect is significantly better than that of the single drug.

The invention relates to the technical field of drug synthesis, and particularly provides a phospholipid-polyethylene glycol modified mono-iodine aza-BODIPY photosensitizer as well as a synthesis method and application thereof, and the phospholipid-polyethylene glycol modified mono-iodine aza-BODIPY photosensitizer has good photosensitization activity and tumor inhibition activity, and can be used for preparing drugs for treating tumors. The compound can be developed into efficient cell membrane targeted photodynamic antitumor drugs, and can be used for photodynamic therapy of non-specific wide tumors, such as pancreatic cancer, cervical cancer, brain glioma, lung cancer, gastric cancer, bladder cancer, ovarian cancer, colon cancer, skin cancer, prostatic cancer and the like.

The present application is suitable for the technical field of tumor treatment drugs, and provides a metal cobalt-based single-atom nanozyme particle loaded with siRNA and a preparation method and application thereof. The particle is formed by electrostatic combination of siRNA for knocking down a c-Myc gene and a polyethylene glycol modified cobalt-based single-atom nanozyme, wherein the cobalt-based single-atom nanozyme is prepared by calcining Co-doped ZIF-8, cobalt elements are dispersed in a nitrogen-doped carbon carrier in the form of single atoms, and cobalt-nitrogen coordination active sites are formed. The preparation process includes three steps of synthesis of the cobalt-based single-atom nanozyme, polyethylene glycol modification and siRNA loading, and is simple in process, convenient in operation, does not require complex and expensive equipment, and is easy for industrialized production. It is verified by experiments that the particle has reliable safety and significant effectiveness in the treatment of cholangiocarcinoma, can effectively inhibit the growth of cholangiocarcinoma solid tumors, is suitable for the treatment of malignant tumors, and has a broad application prospect.

The invention belongs to the technical field of active polypeptides, and particularly relates to a polyethylene glycol modified anti-tumor active polypeptide and application thereof. The modified polypeptide provided by the invention has unexpected better anti-tumor activity, in-vivo stability and low hemolytic activity, and has good application potential.

Nanoparticles useful for drug delivery are described. In one aspect, the nanoparticles contain poly(amine-co-ester)s or poly(amine-co-amide)s (PACE) modified with poly(ethylene glycol) (PACE-PEG), and can be optionally blended with a second PACE polymer optionally containing endgroup modifications. In another aspect, the nanoparticles contain a core containing a PACE polymer optionally containing endgroup modifications, and a polymeric surfactant non-covalently conjugated to the surface of the nanoparticles. The nanoparticles contain a peptide or protein targeting moiety that is covalently conjugated to the PACE-PEG polymer or to the surfactant on the surface of the nanoparticles via a linkage that contains a succinimide or substituted sulfone moiety, respectively. The nanoparticles provide as a versatile platform for the delivery of nucleic acids, such as mRNA.

The present application relates to the technical fields of nanomedicine and functional nanomaterials, and discloses a mesoporous core-shell structure nanoenzyme with multiple enzyme activities as well as a preparation method and application thereof, the nanoenzyme takes gold nanorods as an inner core and iridium and its oxide as an outer shell, the outer shell is modified by polyethylene glycol and then loaded with a photosensitizer chlorin e6 to form an AuNR@Ir / IrO2-PEG / Ce6 nanoenzyme, the nanoenzyme has high catalase (CAT) activity, can decompose hydrogen peroxide (H2O2) in a tumor microenvironment and generate oxygen (O2) in situ, effectively improves the treatment effect of photodynamic therapy (PDT), and through the synergistic effect of photodynamic therapy (PDT) and photothermal therapy (PTT) combined with photoacoustic imaging function, provides a brand-new diagnosis and treatment integrated solution for efficient treatment and microenvironment regulation of tumors, and effectively makes up for the defects of insufficient enzyme activity and low photothermal conversion efficiency of the prior art.

The present application relates to the field of biological medicine, and more particularly to a nano-composite system for treating vitiligo, an intelligent controlled-release microneedle patch and a preparation method thereof, wherein the nano-composite system contains enzyme-responsive micelles and polyethylene glycol modified polydopamine nanoparticles; the enzyme-responsive micelles are formed by glyceryl monostearate encapsulating ginseng root-derived exosomes; and the polyethylene glycol modified polydopamine nanoparticles are formed by covalent connection of a polydopamine core and methoxy-polyethylene glycol-amine. Compared with the prior art, the nano-composite system of the present application can realize the spatiotemporal programmed release of drugs in response to the pathological microenvironment, wherein the polydopamine nanoparticles provide instant antioxidant effect, and the exosome micelles trigger release under the condition of overexpression of matrix metalloproteinase-9, thereby synergistically exerting anti-inflammatory and promoting pigment regeneration effects; animal experiments have proved that the patch can effectively reverse the white spots and realize the recoloring of hair and skin. The microneedle patch thus prepared can realize transdermal self-administration, is convenient and painless.

The present invention discloses a thrombus-targeting self-sensitizing hybrid nano-assembly, its preparation method and application, belonging to the technical field of biomedicine. Specifically, it relates to the synthesis of antiplatelet prodrugs, the preparation method and application of a nano-assembly formed by co-assembling an antiplatelet prodrug modified with a polyethylene glycol modifier linked by a thrombus-targeting peptide and a photothermal / photodynamic bifunctional photosensitizer. The antiplatelet prodrug and the photosensitizer molecule are co-assembled to form a nano-assembly through non-covalent forces. The surface of the nano-assembly is modified with a polyethylene glycol modifier and a polyethylene glycol modifier linked by a thrombus-targeting peptide. The molar ratio of the antiplatelet prodrug to the photosensitizer is 5:1 to 1:5. The preparation method of the present invention is stable and reliable, the preparation process is simple, and the prepared nano-assembly composed of a thrombus-targeting, antiplatelet prodrug and a photothermal / photodynamic bifunctional photosensitizer has a super-high drug loading capacity, and can achieve programmed synergistic treatment of prodrug activation-antiplatelet-photothermal thrombolysis.

The application discloses a slow-release injectable conductive hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedical materials, wherein the components of the slow-release injectable conductive hydrogel comprise crocetin modified by polyethylene glycol with different molecular weights, sodium alginate and bioactive glass; the crocetin modified by polyethylene glycol is prepared by an amidation reaction of crocetin and methoxypolyethylene glycol amine, and different molecular weight crocetin modified by polyethylene glycol is obtained by changing the molecular weight of the methoxypolyethylene glycol amine. The hydrogel can realize gradient slow release of the active ingredient crocetin, matches the conductivity with the natural myocardial tissue, can bridge the electrical signal conduction of the infarction area, realizes the electrophysiological-histological synergistic repair in the treatment of myocardial infarction, and provides an effective treatment strategy for the treatment of myocardial infarction.

The application provides a preparation method of rod-shaped and four-foot-shaped gold nanocapsules, and belongs to the field of interface modification. The method first prepares CTAB-stabilized rod-shaped / four-foot-shaped gold nanoparticles, replaces the CTAB with a mercapto-polyethylene glycol amino ligand to obtain polyethylene glycol modified gold nanoparticles with amino groups at the ends, then adds N-hydroxysuccinimide acrylate into the solution to react with the amino groups on the surface of the gold nanoparticles to obtain polyethylene glycol modified gold nanoparticles with double bonds at the ends, and finally adds a reaction monomer, a crosslinking agent and an initiator into the solution to perform in-situ radical polymerization to prepare the gold nanocapsules with a core-shell structure. The application modifies a crosslinked polymer shell layer on the surface of the rod-shaped and / or four-foot-shaped gold nanoparticles, so that the gold nanoparticles have high stability and can maintain the structural integrity in a physiological environment.

The present invention relates to polyethylene glycol-modified form of kinin or derivative thereof and pharmaceutical use thereof. In one aspect, by PEG modification technology, the half-life of the kinin is greatly prolonged, meanwhile, the biological activity of the kinin is exerted, and the problem that pure kinin has extremely short half-life and therefore has no druggability is solved. PEG modifiers used include, but are not limited to, SPA, SCM and SS modifiers, and can be linear or branched PEG modifiers. In another aspect, the kinin variant has obvious advantages, such as the half-life is further prolonged while the affinity activity with receptor is maintained, and the in vivo efficacy of the PEG-modified kinin variant is superior to that of PEG-modified wild-type kinin, so that the druggability of the kinin is further improved. In addition, the PEG-modified kinin show remarkable efficacy in various administration routes, especially in subcutaneous injection and oral administration, which is convenient in clinical use, and effectively improves the compliance of a patient, and is more suitable for long-term therapeutic administration, such as the convalescent treatment, relapse prevention and the like of a patient with cerebral stroke.

The application provides a 1,2-dithiolane-based dynamic covalent hemostatic hydrogel and a preparation method and application thereof, the dynamic covalent hemostatic hydrogel is composed of a cross-linked dynamic polymer network, the cross-linked dynamic polymer network is prepared through ring-opening polymerization of a mixture containing the following components: a polyethylene glycol modified thioctic acid derivative TA-PEG, a tris(hydroxymethyl)aminomethane modified thioctic acid derivative TA-Tris, and a thioctic acid derivative TOS modified with a sulfonic acid group and a quaternary ammonium cation. The application provides a multifunctional dressing capable of considering the ultra-high swelling liquid absorption capacity, the strong wet surface interfacial adhesion, the structural mechanical stability and the persistent broad-spectrum antibacterial performance in a complex, dynamic and large amount of exudate accompanied wound surface environment.

The invention provides a preparation method and application of an acid-responsive extracellular vesicle. The method comprises the following steps: separating the extracellular vesicle from a biological source material through a differential centrifugation method and an ultracentrifugation method; and carrying out acid responsive modification of polyethylene glycol on the extracellular vesicles by using a Schiff base bond. The preparation method is mild in reaction conditions, does not need strong acid or strong alkali conditions, and can maintain the stability of EVs. Meanwhile, polyethylene glycol modification can prolong the in-vivo circulation time of the extracellular vesicles, and the extracellular vesicles have acid response capability and can generate response bond breaking reaction and release in a weak acid microenvironment of tumors to achieve the aim of tumor targeting.

The application discloses a method for hydrophilic modification of halenaquinone by carboxyl polyethylene glycol with a benzo cyclooctyne group to obtain benzo cyclooctyne-polyethylene glycol-halenaquinone (CPH) with good biocompatibility, and uses CPH for anti-tissue fibrosis treatment through glycometabolism engineering and orthogonal click chemistry. Compared with halenaquinone small molecules, CPH has negligible cytotoxicity. CPH has good therapeutic effect on tissue fibrosis in combination with glycometabolism engineering.