77 results about "WAS PROTEIN" patented technology

The invention provides a preparation method of a calcium emulsifying and embedding system with high bioaccessibility, which comprises the following steps: S1) heating and mixing biomacromolecules and an emulsifier in water to obtain a water phase; the biomacromolecules are protein colloids and / or polysaccharide colloids; the method comprises the following steps: heating and mixing a calcium source substance and anhydrous cream to obtain an oil-solid phase; and S2) mixing the oil-solid phase with a water phase, and emulsifying to obtain the calcium emulsifying and embedding system. Compared with the prior art, the preparation method has the advantages that the natural macromolecular protein and / or polysaccharide with high nutritional value is used as a carrier, the calcium source substance is embedded through a water-oil-solid three-phase emulsification technology, and the preparation method is simple, easy to operate, low in cost and environmentally friendly; in addition, a water-oil-solid three-phase homogenized emulsion is created, the problem that the dispersion stability of milk mineral salt in a liquid food matrix is poor is effectively solved, release of free calcium ions in the small intestine environment can be promoted, and the calcium bioaccessibility is remarkably improved.

Disclosed are protein-like polymers and uses thereof. The protein-like polymers generally comprise a polymer of formula (FX1). The polymer of formula (FX1) in some aspects comprises a peptide that (i) inhibits aggregation of, (ii) accelerates aggregation of, (iii) binds to, and / or (iv) mimics: (a) at least a portion of Tau protein, and / or (b) at least a portion of microtubulin protein.

Provided herein are proteins comprising a viral-binding domain and reagents comprising same. In some aspects, the provided proteins and reagents can be used for the purification of viral particles. In some aspects, the provided proteins and reagents improve the efficiency with which target cells can be transduced. Also provided herein are methods using the provided proteins and reagents, including for purifying viral particles or for transducing cells. Related kits and articles of manufacture are also provided.

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

Disclosed are protein switches, components of such protein switches, and uses thereof, which can sequester biologically active peptides and / or binding domains, keeping them in an inactive ("off") state until combined with a second, designed polypeptide, termed a key, thereby inducing a conformational change that activates ("on") the biologically active peptide or binding domain.

The present invention is a multivalent immunogenic composition for immunizing an animal against filariasis. In some embodiments, the antigens of the multivalent immunogenic composition are protein-based, DNA-based, or a combination thereof. This invention also provides a method and kit for detecting a filarial nematode and determining vaccine efficacy.

In one aspect, the invention provides a peptide comprising a chaperone-mediated autophagy (CMA)-targeting signal domain; a protein-binding domain that selectively binds to a target cytosolic protein; and a cell membrane penetrating domain (CMPD). In another aspect, the invention provides methods for reducing the intracellular expression level of an endogenous target protein in vitro and in an animal, wherein the method involves administration of the peptide. Methods are also provided for treating a pathological condition in an animal, the methods comprising administering the peptide to the animal. In one embodiment, the pathological condition is a neurodegenerative disease. In another embodiment of the invention, the target cytosolic protein is death associated protein kinase 1 and the CMPD is protein transduction domain of the HIV-1 Tat protein.

The present invention is in the field of recombinant protein, in particular protein, such as antibody, manufacturing.

Methods for determining a representation of a protein complex, given a constituent target complex of that protein complex are presented; where the constituent target complex is a single entity constituent or subcomplex of the protein complex; and wherein a protein complex is a complex of some combination of one or more of proteins, nucleic acids, metal ions, and small molecules. A recursive neural network is devised, wherein for each iteration of the recursion, a representation of the output constituent of the protein complex together with the input constituent target complex is passed into the neural network as input for the next iteration. Some embodiments of the invention include design and manufacturing of effective synthetic biologic drugs, monoclonal antibody (mAb) drug, Antibody Drug Conjugate (ADC), peptide ligand drug, and small molecule drugs (SMDs).

The present invention provides a method of transpeptidation, a transpeptidation product obtainable from the method, and a chimeric fusion polypeptide. An exemplary method of transpeptidation includes linking a donor substrate to a recipient protein or peptide using a transpeptidase, where the donor substrate is a protein, peptide, amino acid amide, or amino acid ester, and where the linking reaction produces a linking product and a by-product as a substrate for the transpeptidase; and cleaving one or more amino acids from the end of the by-product using an exopeptidase such that the truncated by-product is not a substrate for the transpeptidase, thereby moving the reaction equilibrium of the transpeptidation reaction to the ligation product.

The present disclosure relates to plant-based ingredients, particularly protein ingredients, that are suitable for use as processing intermediates in a variety of alternative consumable applications, including vegetarian or vegan analogs, and methods of making the same. The plant-based ingredients have desirable organoleptic properties that result from reduced saponin content in the plants or plant parts from which they are obtained. The present disclosure includes ingredients and products made by using such plants and plant parts, as well as related methods and compositions associated therewith.

The present disclosure provides systems and methods for measuring one or more analytes at the single cell level. In some instances, sequencing of a composite barcode sequence may identify a type of analyte, identify a cell, and determine that the type of analyte originated from the cell. In some instances, a probability that a type of analyte originated from the cell may be determined. Multiple types of analytes may be identified to have originated from the cell, and / or the respective probabilities determined. An analyte may be a protein, such as a surface-bound protein or an internal protein. An analyte may be a metabolite or other small molecule. An analyte may be any constituent of a cell.

The utility model discloses an online ultrafiltration permeate liquid protein leakage monitoring system belongs to the biopharmaceutical technical field. The system includes ultrafilter, nondestructive protein concentration detection subassembly and alarm component, be equipped with drainage pipe on the ultrafilter, nondestructive protein concentration detection subassembly is established on the drainage pipe, the alarm component is electrically connected with nondestructive protein concentration detection subassembly through the controller. Through nondestructive protein concentration detection subassembly real -time, nondestructive monitoring the protein concentration of filtrate in drainage pipe, and will relevant protein concentration signal transmission to controller, when there is protein leakage, the controller controls the alarm component and sends the alarm. Realized real -time protein concentration monitoring to filtrate, can in time, accurate discovery protein leakage, realizes nondestructive protein concentration monitoring to filtrate simultaneously, avoids causing product loss, ensures product yield, no longer uses trichloroacetic acid and detects protein concentration, significantly reduced the biopharmaceutical workshop pollution risk.

The invention relates to collagenase as well as a coding gene and application thereof. The collagenase provided by the invention is a protein (a) or (b), wherein (a) is a protein composed of an amino acid sequence as shown in SEQ ID No: 1; and (b) a protein which is obtained by substituting, deleting or adding one or more amino acids in the amino acid sequence in (a), has collagen hydrolysis or fat dissolving activity and is derived from (a). The collagenase provided by the invention can be applied to cell separation and tissue digestion, fat dissolution or collagen dissolution, and can also be applied to preparation of medicines for treating Doltt's contracture, Petroleum disease and lumbar disc herniation and degreasing in the field of beauty.

The invention discloses a light-controlled protein loading and releasing platform based on bent nanowires and a preparation method and application thereof. The preparation method comprises five steps of mask preparation, bent nanowire preparation, amination treatment, photo-acid modification and antibody attachment. The platform uses the bent nanowire as the substrate, has the characteristics of high specific surface area, nano confinement effect and high absorptivity, and can obviously improve the maximum capacity, so that the platform is easy to absorb both low-abundance protein and high-abundance protein. Furthermore, due to the fact that the target biomolecule is protein, by means of the principle that specific adsorption of the target biomolecule is sensitive to the pH value of the environment, a reversible photoacid molecule is used as a light-operated gate in the preparation method, the local pH value is adjusted to control specific adsorption and dissociation of the protein and the antibody, and the specific adsorption and dissociation of the protein and the antibody are controlled. And due to the high absorptivity of the bent nanowires, the bent nanowires can be dissociated in an extremely short time, and the local pH value is reduced, so that the protein is quickly released.

Described herein are protein-drug conjugates and compositions thereof that are useful, for example, for target-specific delivery of therapeutic moieties, e.g., camptothecin analogs and / or derivatives. In certain embodiments, provided are specific and efficient methods for producing protein-drug constructs (e.g., antibody-drug conjugates) utilizing a combination of transglutaminase and 1,3-cycloaddition techniques. Camptothecin analogs, antibody-drug conjugates, and compositions which comprise glutaminyl-modified antibodies and camptothecin analog payloads and are provided.

The invention relates to an insecticidal protein and application thereof in prevention and treatment of lepidoptera insects. The insecticidal protein is a protein I and / or a protein II, the protein I is a first mutant protein of which the structural domain II of Cry2A protein is the structural domain II of Anc0 protein, and the amino acid sequence of the Anc0 protein is as shown in SEQ ID No.8; and the protein II is a second mutant protein obtained by mutating the Cry2A protein at at least one of Q318I, A337S, S360 N, G384E and L459A.

Described herein are protein-payload conjugates and compositions thereof that are useful, for example, for target-specific delivery of therapeutic and / or imaging agent moieties. In certain embodiments, provided are specific and efficient methods for producing protein-payload constructs (e.g., antibody-drug conjugates) utilizing a combination of transglutaminase and Diels-Alder techniques. Antibody-drug conjugates and compositions which comprise glutaminyl-modified antibodies, Diels-Alder adducts, and reactive payloads and are provided.

Disclosed herein is a soluble protein signature from the tumor microenvironment that can predict overall survival post-surgery in pancreatic adenocarcinoma. The disclosed protein signatures provide a precision approach to surgical therapy for patients with pancreatic cancer. Also disclosed herein is a soluble protein signature from the tumor microenvironment that can diagnose pancreatic ductal adenocarcinoma (PDAC). Also disclosed herein are proteins that can be used to accurately normalize protein levels in a pancreatic sample.

Provided herein are proteins that inhibit both the metalloprotease activity and disintegrin activity of human ADAM8, wherein the protein comprises an antigen-binding domain that: (i) binds specifically to human ADAM8; and (ii) binds to an epitope within human ADAM8 that includes at least one amino acid within the sequence of SEQ ID NO: 1, nucleic acids, vectors, compositions, and methods of use thereof (e.g., methods of treatment and methods of diagnosing).

The invention relates to an insecticidal protein and application thereof in prevention and treatment of lepidoptera insects. The insecticidal protein is a protein I and / or a protein II, the protein I is a first mutant protein of which the structural domain II of Cry2A protein is the structural domain II of Anc0 protein, and the amino acid sequence of the Anc0 protein is shown as SEQ ID No.8; and the protein II is a second mutant protein obtained by mutating the Cry2A protein at at least one of Q318I, A337S, S360 N, G384E and L459A.

Provided herein are methods and compositions relating to glucagon-like peptide-1 receptor (GLP1R) libraries having nucleic acids encoding for immunoglobulins that bind to GLP1R. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

The application discloses a hydroxylase and application thereof in synthesis of 14-deoxoandrographolide. The hydroxylase is protein CYP72A399, and the amino acid sequence of the protein CYP72A399 is shown as SEQ ID No: 2. Experiments prove that the protein CYP72A399 can be used as a hydroxylase to catalyze new andrographolide aglycone to generate 14-deoxoandrographolide. Therefore, the protein CYP72A399 has important theoretical and practical significance for synthesis of 14-deoxoandrographolide and cultivation of high-quality andrographis paniculata. The application has important application value.

Methods, compositions, kits, and systems are provided for identifying protein-binding regions in genomic DNA. The method may include contacting genomic DNA with an adenine methyltransferase (A-MTase), wherein the A-MTase methylates adenine residues in a region of the genomic DNA that is not protein-binding; and performing single-molecule long-read sequencing on the contacted genomic DNA to detect locations in the genomic DNA lacking methylated adenine residues, thereby identifying regions in the genomic DNA that are protein-binding. The bound region may be a nucleosome location, and the method can determine the nucleosome location in the genomic DNA. A method is also provided for visualizing chromatin regions that are not protein-binding and spatially serve as substrates for the A-MTase within the cell by visualizing the location of methylated adenine after contacting the cell with the adenine methyltransferase (A-MTase).

The present disclosure relates to Wiskott-Aldrich Syndrome (WAS) binding proteins and uses thereof. The present disclosure further relates to WAS protein variants thereof and methods of producing WAS binding proteins thereof.

Described herein is a process for the preparation of plant protein-based microcapsules. Also described herein are protein-based microcapsules and perfuming compositions and consumer products including the microcapsules, in particular perfumed consumer products in the form of home care or personal care products.

The present disclosure relates a conjugate having the structure (I): or a pharmaceutically acceptable salt or solvate thereof, wherein: RBM is a receptor binding molecule; L is a linker bound to RBM and M; E is a spacer; W is a moiety which, after cleavage of the group Z is capable of forming a ring together with the spacer E, Y1 and the phosphorus; Z is a cleavable group; HC is a molecule comprising a 4 to 20 membered heterocyclic ring comprising the groups LE, PBL, XE1 and RE1, LE is a linker bound to the 4 to 20 membered heterocyclic ring and to PBL, or LE is a linker bound to PBL and RE1; PBL is a protein binding ligand and n is an integer ranging from 1 to 20. The conjugates are useful in the treatment of cancer.

This invention discloses an AI-2 quenching enzyme and its application. The AI-2 quenching enzyme is protein QQ-5, with the amino acid sequence shown in SEQ ID NO:1. Experiments have demonstrated that protein QQ-5 possesses AI-2 quenching enzyme activity, which can significantly improve the disease resistance of aquatic animals to Vibrio, manifested in effectively increasing the survival rate of aquatic animals infected with Vibrio and delaying the time to death. Therefore, protein QQ-5 plays an important role in the control of AI-2 family signaling-mediated pathogenic bacteria in aquatic animals. This invention has significant application value.

Provided herein are protein-like polymers for tuning tau activity. In aspects, provided herein are protein-like polymers for inhibiting tau fibril propagation in a subject.