158 results about "WAS PROTEIN" patented technology

The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses means and methods for single molecule protein sequencing and / or amino add identification using cleavage inducing agent. Said cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics of said reaction.

The invention provides a preparation method of a calcium emulsifying and embedding system with high bioaccessibility, which comprises the following steps: S1) heating and mixing biomacromolecules and an emulsifier in water to obtain a water phase; the biomacromolecules are protein colloids and / or polysaccharide colloids; the method comprises the following steps: heating and mixing a calcium source substance and anhydrous cream to obtain an oil-solid phase; and S2) mixing the oil-solid phase with a water phase, and emulsifying to obtain the calcium emulsifying and embedding system. Compared with the prior art, the preparation method has the advantages that the natural macromolecular protein and / or polysaccharide with high nutritional value is used as a carrier, the calcium source substance is embedded through a water-oil-solid three-phase emulsification technology, and the preparation method is simple, easy to operate, low in cost and environmentally friendly; in addition, a water-oil-solid three-phase homogenized emulsion is created, the problem that the dispersion stability of milk mineral salt in a liquid food matrix is poor is effectively solved, release of free calcium ions in the small intestine environment can be promoted, and the calcium bioaccessibility is remarkably improved.

The invention relates to protein peptide and polysaccharide efficient separation equipment for sea cucumber peptide processing and a use method of the protein peptide and polysaccharide efficient separation equipment, and particularly relates to the technical field of bioengineering. The sea cucumbers are subjected to two-time enzymolysis by adopting two-time enzyme adding and two-time different hydrolase adding in cooperation with different enzymolysis conditions, a first ultrafiltration membrane with the molecular weight cut-off of 10 KDa is selected for first-time ultrafiltration separation, permeate liquid is protein peptide mixed liquid, cut-off liquid is polysaccharide mixed liquid, and the protein peptide mixed liquid and the polysaccharide mixed liquid are mixed. The protein peptide mixed solution and the polysaccharide mixed solution correspondingly enter corresponding ultrafiltration assemblies to be subjected to multi-stage ultrafiltration, protein peptide and polysaccharide are further separated and purified respectively, and finally the protein peptide mixed solution and the polysaccharide mixed solution enter corresponding spray drying towers to be subjected to spray drying to obtain corresponding protein peptide powder and polysaccharide powder; separation, purification and drying of protein peptide and polysaccharide can be achieved at the same time, and waste of resources is reduced.

Provided herein are co-assembling peptides which may form granules under stimulating conditions. Also provided herein are protein carrying granules. Further provided herein, are methods of making each of the co-assembling peptides and granules.

Disclosed are processes for producing protein products, including protein concentrates from biomass material comprising Rubisco protein. Also disclosed are protein concentrates with advantageous functional properties, related protein products, and food products comprising protein concentrates.

Disclosed are protein-like polymers and uses thereof. The protein-like polymers generally comprise a polymer of formula (FX1). The polymer of formula (FX1) in some aspects comprises a peptide that (i) inhibits aggregation of, (ii) accelerates aggregation of, (iii) binds to, and / or (iv) mimics: (a) at least a portion of Tau protein, and / or (b) at least a portion of microtubulin protein.

Described herein are protein-drug conjugates and compositions thereof that are useful, for example, for targeting glucagon-like peptide 1 receptor (GLP1R). In certain embodiments, provided are peptidomimetic payloads and linker-payloads and methods of making same. More specifically, GLP1 peptidomimetics, antibody-drug conjugates, and compositions which comprise anti-GLP1R antibodies and GLP1 peptidomimetic payloads and methods of treating GLP1R-associated conditions are provided.

Provided are protein nanostructures that display Neisseria meningitidis factor H binding protein (fHBP). The protein nanostructure may be a two-component icosahedral nanostructure. Further provided are vaccine compositions, methods of manufacturing, and methods of use, e.g., immunizing a subject to generate a protective immune response to Neisseria meningitidis.

Provided are mRNA for protein expression and a template therefor, particularly, provided are an mRNA transcription vector comprising a gene construct according to one embodiment, a method of preparing an mRNA molecule, the method comprising the step of performing transcription using the mRNA transcription vector, an mRNA molecule prepared by the method, and a pharmaceutical composition comprising the mRNA molecule as an active ingredient.

The invention discloses a transcription factor GPC6 for regulating and controlling the protein content of rice grains and application of the transcription factor GPC6. The amino acid sequence of the transcription factor is shown as SEQ ID NO.1. The transcription factor comprises a genome region and a promoter, the nucleotide sequence of the genome region is shown as SEQ ID NO.2, the nucleotide sequence of the promoter is shown as SEQ ID NO.3, the coding sequence (CDS) is shown as SEQ ID NO.4, and the coding sequence (CDS) is shown as SEQ ID NO.3. The transcription factor GPC6 can be used for regulating and controlling the protein content of the rice grains. And comprises all alleles thereof. Whole genome association analysis is carried out on the glutelin content and the total protein content, and a site GPC6 capable of being repeatedly detected is identified on a No.6 chromosome. Gene cloning is carried out, gene resources for rice quality genetic improvement can be enriched, and help is provided for rice quality improvement, especially protein content improvement.

Provided herein are proteins comprising a viral-binding domain and reagents comprising same. In some aspects, the provided proteins and reagents can be used for the purification of viral particles. In some aspects, the provided proteins and reagents improve the efficiency with which target cells can be transduced. Also provided herein are methods using the provided proteins and reagents, including for purifying viral particles or for transducing cells. Related kits and articles of manufacture are also provided.

An oleogel and a preparation method therefor and a use thereof. The oleogel comprises 60-90 wt % of liquid oil, 6-35 wt % of a structuring agent, and 4-15 wt % of water. The liquid oil comprises an unsaturated fatty acid, and the structuring agent is a protein denatured particle. The oleogel is an antioxidant edible oil composition rich in unsaturated fatty acids, an additional stabilizer does not need to be added, the use of a thickener, an emulsifier, and an organic reagent is avoided, and the oleogel has oxidation resistance and is beneficial to long-term storage of products. The oleogel can be used as an oral pharmaceutical preparation to achieve various therapeutic utility.

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

Disclosed herein are protein-based compositions and conjugates thereof that can take advantage of beneficial delivery properties to improve vaccine efficacy. An example protein-based composition includes an albumin-binding nanobody and a peptide antigen domain. An example conjugate includes the protein-based composition attached to a drug through a linker. Also disclosed are methods of making and using the protein-based compositions and conjugates thereof.

Disclosed are protein switches, components of such protein switches, and uses thereof, which can sequester biologically active peptides and / or binding domains, keeping them in an inactive ("off") state until combined with a second, designed polypeptide, termed a key, thereby inducing a conformational change that activates ("on") the biologically active peptide or binding domain.

The present invention provides methods for quantifying and / or screening for the formation of complexes, in which two of the three compounds of the complex are proteins, one of the two tagged with a fluorescent label and the other of the two tagged with a fluorescence quencher. The third compound is a compound capable of linking those two proteins together, thus forming a ternary complex. The methods of the present invention consist of analysing the changes in fluorescence spectrum that occur following the formation of a complex comprising said compounds, due to the interaction between fluorescent label and fluorescence quencher that formation of said complex brings.

Compounds comprising aminoindole carboxamide, compositions comprising same, and method of using such compounds and compositions to inhibit tubulin-associated unit (tau) protein aggregation or alpha-synuclein (a-syn) protein aggregation in a subject having, or at risk for, tau protein aggregation or a-syn protein aggregation, respectively.

The present invention relates to a method of making a frozen or refrigerated food product, comprising combining a first food component with at least a second food component to produce the frozen or refrigerated food product, the first food component comprising extruded pieces that include protein granules with an average size greater than 75µm within a protein matrix comprising a protein matrix disruptive ingredient forming a protein matrix composition having a protein content of from about 10% to about 65% by weight, a moisture content of from about 25% to about 55% by weight and an oil content of from about 1% to about 15% by weight, the oil being substantially uniformly distributed throughout the extruded pieces, wherein the method comprises: processing the protein matrix composition under extrusion conditions comprising a specific mechanical energy (SME) of from about 8 Wh / kg to about 100 Wh / kg, a die pressure of from about 150 PSI to about 1000 PSI, and a die temperature of from about 200 °F to about 350 °F.

The present invention is a multivalent immunogenic composition for immunizing an animal against filariasis. In some embodiments, the antigens of the multivalent immunogenic composition are protein-based, DNA-based, or a combination thereof. This invention also provides a method and kit for detecting a filarial nematode and determining vaccine efficacy.

In one aspect, the invention provides a peptide comprising a chaperone-mediated autophagy (CMA)-targeting signal domain; a protein-binding domain that selectively binds to a target cytosolic protein; and a cell membrane penetrating domain (CMPD). In another aspect, the invention provides methods for reducing the intracellular expression level of an endogenous target protein in vitro and in an animal, wherein the method involves administration of the peptide. Methods are also provided for treating a pathological condition in an animal, the methods comprising administering the peptide to the animal. In one embodiment, the pathological condition is a neurodegenerative disease. In another embodiment of the invention, the target cytosolic protein is death associated protein kinase 1 and the CMPD is protein transduction domain of the HIV-1 Tat protein.

The present invention is in the field of recombinant protein, in particular protein, such as antibody, manufacturing.

The invention discloses an engineered S-layer and a preparation method thereof, an HEMT sensor based on in-vitro self-assembly of the engineered S-layer and a preparation method of the HEMT sensor and a biomolecule detection system, the engineered S-layer is formed by fusion expression of S-layer protein based on recombination or redesign and a biological sensitive element, and the biological sensitive element is protein or polypeptide; the HEMT sensor based on engineering S-layer in-vitro self-assembly comprises a field effect transistor body and a grid sensitive region, the field effect transistor body comprises a source electrode and a drain electrode which are arranged on the two sides respectively. The grid electrode sensitive region is arranged between the source electrode and the drain electrode and comprises a semiconductor material for forming a heterojunction structure and a two-dimensional protein nano array containing a biological sensitive element, and the two-dimensional protein nano array is formed by in-vitro self-assembly of an engineered S-layer on the surface of the semiconductor material. The biomolecule detection system comprises an HEMT sensor based on engineering S-layer in-vitro self-assembly, an electrical detection system and a signal processing and analyzing system.

The present invention is situated in the field of multimers used for targeted therapies. More particularly, the present invention provides a dimeric protein complex comprising a first polypeptide comprising a first functional component and the C-terminal fragment of the C4bp beta-chain, wherein said first functional component is coupled to the C-terminus of the C-terminal fragment of the C4bp beta-chain; and a second polypeptide comprising a second functional component and the C-terminal C4bp beta-chain, wherein said second functional component is coupled to the C-terminus of the C-terminal fragment of the C4bp beta-chain; wherein said first and second polypeptides are identical or different and wherein said first and second functional components are proteins or polypeptides. Further provided are nucleic acids encoding said first or second polypeptide of the dimeric protein complex and pharmaceutical compositions comprising the dimeric protein complex. The invention also relates to the use of the dimeric protein complexes in immunotherapy.

Methods for determining a representation of a protein complex, given a constituent target complex of that protein complex are presented; where the constituent target complex is a single entity constituent or subcomplex of the protein complex; and wherein a protein complex is a complex of some combination of one or more of proteins, nucleic acids, metal ions, and small molecules. A recursive neural network is devised, wherein for each iteration of the recursion, a representation of the output constituent of the protein complex together with the input constituent target complex is passed into the neural network as input for the next iteration. Some embodiments of the invention include design and manufacturing of effective synthetic biologic drugs, monoclonal antibody (mAb) drug, Antibody Drug Conjugate (ADC), peptide ligand drug, and small molecule drugs (SMDs).

The present invention provides a method of transpeptidation, a transpeptidation product obtainable from the method, and a chimeric fusion polypeptide. An exemplary method of transpeptidation includes linking a donor substrate to a recipient protein or peptide using a transpeptidase, where the donor substrate is a protein, peptide, amino acid amide, or amino acid ester, and where the linking reaction produces a linking product and a by-product as a substrate for the transpeptidase; and cleaving one or more amino acids from the end of the by-product using an exopeptidase such that the truncated by-product is not a substrate for the transpeptidase, thereby moving the reaction equilibrium of the transpeptidation reaction to the ligation product.

Disclosed herein are food products comprising a non-animal-origin protein and methods for producing the food products, wherein the non-animal-origin protein may not be a protein or a fragment or truncation of a protein obtained from an animal.

The present disclosure relates to plant-based ingredients, particularly protein ingredients, that are suitable for use as processing intermediates in a variety of alternative consumable applications, including vegetarian or vegan analogs, and methods of making the same. The plant-based ingredients have desirable organoleptic properties that result from reduced saponin content in the plants or plant parts from which they are obtained. The present disclosure includes ingredients and products made by using such plants and plant parts, as well as related methods and compositions associated therewith.

The present disclosure provides systems and methods for measuring one or more analytes at the single cell level. In some instances, sequencing of a composite barcode sequence may identify a type of analyte, identify a cell, and determine that the type of analyte originated from the cell. In some instances, a probability that a type of analyte originated from the cell may be determined. Multiple types of analytes may be identified to have originated from the cell, and / or the respective probabilities determined. An analyte may be a protein, such as a surface-bound protein or an internal protein. An analyte may be a metabolite or other small molecule. An analyte may be any constituent of a cell.

The utility model discloses an online ultrafiltration permeate liquid protein leakage monitoring system belongs to the biopharmaceutical technical field. The system includes ultrafilter, nondestructive protein concentration detection subassembly and alarm component, be equipped with drainage pipe on the ultrafilter, nondestructive protein concentration detection subassembly is established on the drainage pipe, the alarm component is electrically connected with nondestructive protein concentration detection subassembly through the controller. Through nondestructive protein concentration detection subassembly real -time, nondestructive monitoring the protein concentration of filtrate in drainage pipe, and will relevant protein concentration signal transmission to controller, when there is protein leakage, the controller controls the alarm component and sends the alarm. Realized real -time protein concentration monitoring to filtrate, can in time, accurate discovery protein leakage, realizes nondestructive protein concentration monitoring to filtrate simultaneously, avoids causing product loss, ensures product yield, no longer uses trichloroacetic acid and detects protein concentration, significantly reduced the biopharmaceutical workshop pollution risk.

The present disclosure provides a method for introducing a target molecule into a lipid vesicle (for example, an extracellular vesicle). The present disclosure also provides a lipid vesicle (for example, an extracellular vesicle) produced using the method for introducing a target molecule into a lipid vesicle (for example, an extracellular vesicle). According to the present disclosure, a complex (a first complex) of a lipid (for example, an ionic lipid; particularly a liquid lipid composition) and a target molecule is fused with a lipid vesicle (for example, an extracellular vesicle) to form a complex (a second complex). The first complex and the second complex are suitable for the introduction of the target molecule into a cell, and the second complex is particularly suitable for the introduction of the target molecule into a cell. The target molecule may be a protein (for example, an antibody), a nucleic acid, or other compounds.