764 results about "WAS PROTEIN" patented technology

Disclosed herein are proteins that include an immunoglobulin fold and that can be used as scaffolds. Also disclosed herein are nucleic acids encoding such proteins and the use of such proteins in diagnostic methods and in methods for evolving novel compound-binding species and their ligands.

Disclosed herein are proteins that include a fibronectin type III domain having at least one randomized loop. Also disclosed herein are nucleic acids encoding such proteins and the use of such proteins in diagnostic methods and in methods for evolving novel compound-binding species and their ligands.

Methods and systems for the discovery of high-affinity peptide ligands and the resulting compositions are described herein. The amino acid sequence of a target protein is used to identify one or more homologous proteins of the target protein. Publications and databases are textmined to retrieve the sequences of peptide ligands that bind to the homologues or the target protein. Complementary proteins, which are proteins that bind to the target or homologous proteins or to DNA, and their target protein- or DNA-binding regions may also be identified. These candidate ligands are predicted to have a high probability of binding to the target protein or the DNA. The library of candidate peptide ligands is modulated by substituting native amino acid residues with suitable amino acids, thus increasing the explored protein space in a knowledge-based manner. Peptides designed in the modulation step are experimentally screened to identify high-affinity binding ligands, and further optimized through iterative application of the modulation and screening steps.

A process for preparation of nutritionally upgraded oilseed meals which are protein and lipid-rich and have a reduced fiber content, and plant oils from oilseeds for use in fish or other non-human animal diets or human foods comprising the steps of: providing a source of oilseed; subjecting the oilseed to heat treatment to substantially reduce the concentration of at least some antinutritional components normally present in the oilseed to obtain heat-treated seed; dehulling the heat-treated seed to produce a meat fraction, a hull fraction or a mixture thereof; and cold pressing the meat fraction or the mixture to yeild the plant oils and the protein and lipid-rich meals.

The present invention relates to a malleable protein matrice (MPM), which is the reaction product of the agglomeration of proteins after a fermentation process and is exhibiting biological activities and is suitable for the incorporation (or encapsulation) of various hydrophilic or lipophylic substances. The present invention also relates to the process for the preparation of the malleable protein matrice and its usages.

A system for making molecules, and proteins in particular, suitable for detection by a surface-selective nonlinear optical technique. A first use of the invention is for determining a protein's structure in real space and real time. A second use of the invention is to detect a protein or its activity (conformational change). A third use of the invention is for drug screening. A further aspect of the present invention is measuring probe tilt angle orientation in an oriented protein.

To improve the stability of vaccines comprising aluminum salt(s), the invention uses the amino acid histidine. This can improve pH stability and adjuvant adsorption and can be reduce antigen hydrolysis. Histidine is preferably presen during adsorption to the aluminium salt(s). The antigen in the vaccine may be a protein or a saccharide and is preferably from N. meningitidis.

The invention relates to compounds of the formula (I) wherein the moieties R1, R2, R3, R9, R10 and Q and X, Y and Z are as defined in the specification, and salts thereof, as well as their use, methods of use for them and method of their synthesis, and the like. The compounds are protein kinase inhibitors and can, inter alia, be used in the treatment of various proliferative diseases.

The invention discloses a zearalenone toxin gradation enzyme and a coding gene and application thereof. The enzyme is derived from Gliocladiumroseum and is protein in (a) or (b): (a) protein consisting of an amino acid residue sequence of SEQIDNO: 1 in a sequence table; and (b) protein which is derived from the SEQIDNO: 1, has a zearalenone degradation activity and is formed through carrying out substitution and/or deletion and/or addition of one or a plurality of amino acid residues on the amino acid residue sequence of SEQIDNO: 1 in the sequence table. The zearalenone toxin degradation enzyme has highly-efficient degradation effect on zearalenone (ZEN).

The present invention relates to binding proteins specific for VEGF-A, in particular to recombinant binding proteins comprising a binding domain, which inhibits VEGF-Axxx binding to VEGFR-2. Examples of such binding proteins are proteins which comprise an ankyrin repeat domain with the desired binding specificity. The binding proteins are useful in the treatment of cancer and other pathological conditions, e.g. eye diseases such as age-related macular degeneration.

In an embodiment, a number of synthetic protein triblock copolymers are provided comprising first and second end hydrophobic blocks separated by a central hydrophilic block. In particular, the synthetic proteins are elastin-mimetic proteins having improved mechanical characteristics and related methods of making the proteins with the capability of providing precise control over the mechanical properties. Provided are proteins used in a number of medical devices such as artificial blood vessels, shunts, stents or as embolic agents in situations where it is desired to stop or reduce blood flow or pressure in a localized region.

A supramolecule has a first supramolecular component including a first effector molecule covalently joined to a first nucleic acid, and a second supramolecular component including a second effector molecule covalently joined to a second nucleic acid, wherein the second nucleic acid has a region of at least partial complementarity to the first nucleic acid, wherein the first nucleic acid is in a base pairing relationship with the second nucleic acid and the first or second effector molecules are proteins, polypeptides, lipids or sugars. The supramolecule may further have a third supramolecule component which includes a third effector molecule covalently joined to a third nucleic acid, wherein the third nucleic acid has a region of at least partial complementary to the first nucleic acid or the second nucleic acid and wherein the third nucleic acid is in a base pairing relationship with the second nucleic acid or the first nucleic acid.

Herbal cosmetic skin care compositions formulated to combat conditions associated with oxidative stress are provided. The compositions contain effective amounts of one of several active agents, including an herbal agent derived from one or more of the plant species, as well as cosmetically acceptable carriers. Other agents that may be included are proteins, peptides, anti-inflammatory agents, and/or vasodilators. These cosmetic compositions find use in improving the appearance of aged or damaged skin.

The invention relates to a vaccine production technology in the technical field of biology, in particular to a CHO cell strain which is established by utilizing a gene engineering means and is used for expressing recombinant protein PigFC-pigSCFVE2, and a preparation method and application of the recombinant protein. The recombinant fusion protein PigFC-pigSCFVE2 provided by the invention is A1) or A2) shown as follows, wherein A1) is protein of which the amino acid sequence is as shown in SEQ ID No.2, and A2) is protein which is obtained by substituting, losing and/or adding one or several amino acid residues in the amino acid sequence of the protein of the A1) and has PigFC-pigSCFVE2 activity. A monoclonal cell strain which is obtained through the method and capable of carrying out secretory expression on PigFC-pigSCFVE2 is higher in fusion protein expression quantity, fusion protein obtained through affinity separation and purification of an antibody can be combined with a monoclonal antibody, animals can be immunized, the immunity of a generated neutralizing antibody is higher than that of a present market product, the fusion protein can be used for swine classical fever preventive vaccine, and the production cost and the immunity failure loss can be reduced.

Provided herein are methods and compositions relating to G protein-coupled receptor (GPCR) libraries having nucleic acids encoding for a scaffold comprising a GPCR binding domain. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

The present invention is protein feed and its preparation process with potato slag. Protein feed is prepared with waste potato slag from starch production, and through squeezing to dewater, adding certain amount of supplementary material, inoculating certain amount of microbe seed and solid fermentation. The said preparation process is simple, and has high yield, low investment, low power consumption and other advantages. Thus obtained feed product has the fermented product, including microbe, metabolite and substrate utilized fully, and possesses the active components maintained, high amino acid content and high nutritious value.

A non-competitive immunoassay for small analytes, wherein the analyte is reacted with two binding partners. The first binding partner binds to the analyte to form a complex between the first binding partner and the analyte, and the second binding partner binds to the complex formed by the first binding partner and the analyte. The resulting complex formed between the analyte and the binding partners is detected. The binding partners are proteins, such as antibodies including antibody fragments.

The invention discloses a target molecule detection method based on nano-Au and nucleic acid structure. The detection method sequentially comprises the following steps: (1) specific DNA which can specifically react with target molecules is sufficiently hybridized with the cDNA of the DNA to form a double-chain capture probe, wherein the target molecules are proteins or ions; (2) target molecule solution is added for full reaction; (3) nano-Au solution with the mol number of 0.01-1 time of that of the specific DNA is added, and the solution is red after reaction; (4) high salting solution with the final concentration of 1-100 mM is added, and the color change of the solution is observed. The detection method has commonality, wide application range, good specificity and high sensitivity, can quickly detect any proteins or any ions with low cost, and does not need DNA mark or dear instruments.

The present invention relates to a method for detecting immunoblotting based on quantum dot labeling. Firstly, connecting quantum dot with carboxyl or amido with second antibody by chemical reaction to form compound with quantum dot label; employing polyacrylamide gel electrophoresis, analytes is protein, probe is antibody, quantum dot labeled second antibody is used to show color. Protein sample separated by PAGE is transferred to solid carrier, the protein or polypeptide on solid carrier as antigen is processed immunoreaction with corresponding antibody, and then reacted with quantum dot labeled second antibody, under the UV lamp, quantum dot emitting fluorescence to detect electrophoresis separated specific objective gene expression protein component. Immunoblotting image is gained by filter equipped of simple UV imaging system. The present invention employs quantum dot connected with second antibody as excellent fluorescence imaging agent of immunoblotting to enhance the sensitivity and precision.