The invention discloses a high-adhesive one-component deoximation type room-temperature vulcanized silicone rubber sealant comprising the following components from (A) to (F) in parts by weight: 100 parts of modified alpha, omega-dihydroxylpolydimethylsiloxane (A), 10-30 parts of dimethyl silicon oil (B), 7-12 parts of deketoxime crosslinking agent (C), 1-5 parts of silane coupling agent (D), 50-150 parts of reinforcing filler (E) and 0.1-1 part of catalyst (F). Compared with the traditional deoximation type sealant, the sealant has excellent adhesive property particularly for some substrates such as plastics, modified resin and the like which are difficultly adhered by using an organic silicon adhesive and can be widely applied to adhesion and sealing of industries such as electronics, automobiles, household appliances and the like. Modified polydimethylsiloxane is used as a basic polymer in deoximation type silicon rubber, so that the sealant has relatively proper surface drying time, provides more reasonable operation time and meets the production takt requirement under different working conditions; in addition, due to the adoption of the modified polydimethylsiloxane, the sealant has excellent waterproofness and is relatively high in strength retention rate under high temperature and high humidity conditions.