458 results about "Bacillus coli" patented technology

The invention provides a gene, recombinant plasmid and polypeptide for an anti-tumor binary polypeptide. The gene of the recombinant anti-tumor binary polypeptide is obtained by connecting in an operable way the gene of a coding antibody simulator with a recombinant bacillus anthraci protein antigen gene. The recombinant plasmid of the invention is formed by inserting the gene of the coding antibody simulator by double-chain oligomeric nucleotide directed mutagenesis method into the recombinant bacillus anthraci protein antigen gene. The obtained recombinant plasmid is infected into engineering bacillus coli BL-21 to get engineering bacillus coli cell of anti-tumor binary polypeptide; the anti-tumor binary polypeptide can be obtained by expanding the bacillus coli, settling in centrifugal way the bacillus coli body, crushing in altrasonic way, settling and crushing bacillus coli body by hi-speed centrifuging and treating the upper clean solution. The anti-tumor binary polypeptide is of special targeting characteristic, higher efficiency in killing special physical tumor than prior anti-tumor medicine, and will not attack normal cells, and has much lower toxicity and poor-reaction than prior anti-tumor medicine.

The present invention is diphtheria toxin mutant CRM197 and its preparation process, and belongs to the field of immune protein carrier technology. The diphtheria toxin mutant CRM197 is expressed in colibacillus in the form of inclusion body, and the target product has sequence length of 536 amino acids, the immunogenicity the same as that of diphtheria toxin and no toxicity similar to that of diphtheria toxin. The present invention clones gene coding CRM197, constructs recombinant expression plasmid expressing CRM197 and transforms to colibacillus host for expression in the form of inclusion body. The present invention has target protein accounting for over 24 % of total protein content, high CRM197 purity over 95 % and high target protein recovering rate. The preparation process is simple, low in cost and suitable for industrial production.

The invention discloses a long-chain recombination human bone pattern generating protein-2 and preparing method and application, which is characterized by the following: utilizing gene project technique to grow the protein in the expressing system of escherichia coli and bacillus subtilis; adopting human bone sarcoma cell mRNA to do reverse transcription to obtain the cDNA as form; augmenting nucleotide sequence of entire 114 amino acids naturally from carboxyl end; adding a segment of nucleotide in front of the first codon of primer 5' end; increasing a segment of polypeptide at N end corresponding to amino acid sequence; obtaining long-chain rhBMP-2 gene with molecular weight at 30KD and purity over 95%.

The invention belongs to biology technology field, which concretely refers to controlled release tissue factor path inhibitor (LTFPI) and the manufacturing method, and the application. The invention analyzes and experiments the biology information science and structure molecular biology science of tissue factor path inhibitor (TFPI) and its acceptor low density lipoprotein acceptor correspondent protein (LRP), it ascertains the part where the TFPI carboxy end combines with LRP and is eliminated. It designs TFPI carboxy end mutant, which is recombined with primary nucleus or eukarya expressing carrier after constructing LTFPI gene through PCR location, it converts bacillus coli or bici yeast, sifts the high expression project fungus. The primary project fungus are yeasted and expanded, crushed, then they are centrifugated and collects the inclusion body, purifies the LTFPI through molecular sift and ion interchanging two-step method; the eukarya project fungus are carried on with two-step purifying directly. The half-life are prolonged, it has good pour-depressant function.

The invention provides a polyhexamethylene guanidine propionate disinfectant and a preparation method thereof. The polyhexamethylene guanidine propionate disinfectant is a hydrophilic solution prepared by the following steps: adding polyhexamethylene guanidine propionate, citric acid and surfactant to water and uniformly stirring the mixture at normal temperature. The polyhexamethylene guanidine propionate disinfectant has the advantages of broad spectrum of disinfection, quick results, long-time disinfection capability, high safety, non-toxicity, environmental protection, high stability, easy application, quick infiltration and volatilization, no coloration, easy cleaning, and the like. The polyhexamethylene guanidine propionate disinfectant has strong sterilization functions for colon bacillus, staphylococcus aureus, candida albicans, and the like with two-year effective periods. The polyhexamethylene guanidine propionate disinfectant can be used for disinfection of skins, wound surfaces, hands, air, departments of gynecology and urology, drinking water, and the like, thereby being a novel and ideal disinfectant.

The invention relates to a pig breeding and breathing complex virus (PPRSV) and pig 2-type loop virus (PCV 2) series gene recombination adenovirus and vaccine in the field of high and new biotechnology. It uses PCR technology to clone the Cap protein gene into the plasmid carrier pShuttle-CMV-GP5 by open type reading rule and transforms tobacillus coli BJ5183strain with cage carrier pAdEasy-1 to capture the recombination plasmid; it uses recombination plasmid HEK293-A cell to capture recombination adenovirus and purifies it to express the recombination adenovirus rAd-Cap-GP5 of PRRSV GP5protein and PCV-2 Cap protein.

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

The invention discloses an isothermal nucleic acid amplification reaction reagent which comprises Tris buffer solutions, potassium acetate, magnesium acetate, dithiothreitol, polyethylene glycol, adenosine triphosphate (ATP), deoxy-ribonucleoside triphosphate (dNTPs), phosphocreatine, creatine kinase, a primer group, single-strand binding (SSD) protein, colon bacillus helicase RecQ protein, UvsY protein and DNA polymerase. The invention further discloses an isothermal nucleic acid amplification method which includes steps of extracting DNA or performing inverse transcription after DNA extracting, and adding the isothermal nucleic acid amplification reaction reagent and reacting the mixture at a temperature in a range between 25 DEG C and 45 DEG C for 10 minutes to 60 minutes to complete the nucleic acid amplification. Compared with traditional polymerase chain reaction (PCR) technologies and according to the technology, nearly no professional instrument is needed, the operation is simple, technical requirements for operators are low, and the reaction time only needs about half an hour.

The invention belongs to the technical field of microorganism genetic engineering and discloses separation and cloning of nematicidal crystal protein gene originated from thuringiensis and biological activity of the nematicidal crystal protein gene on nematode. In the invention, a new insecticidal crystal protein gene cry1518-35 is obtained by being separated from bacterial strain of activated thuringiensis YBT-1518 of root-knot nematodes in north of China; an encoded product of the gene is a new insecticidal crystal protein Cry1518-35; the protein Cry1518-35 features high insecticidal activity on root-knot nematodes in north of China. The invention also includes the preparation of colon bacillus recombinant bacteria EMB0225 which expresses the insecticidal crystal protein Cry1518-35, the recombinant colon bacillus is preserved in CCTCC, the preservation number is CCTCC NO: M209009.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.

The invention discloses a phosphate tetrahydroberberine drug eutectic and a preparation method thereof. The tetrahydroberberine and phosphate are added and mixed in isopropanol water solution, ethanol water solution or methanol water solution according to the molar ratio; the acquired mixed solution is volatilized under the room temperature. The prepared tetrahydroberberine drug eutectic is a triclinic system, and the space group is P-1, and its axial length (i) is FORMULA; the axial angle is FORMULA. The tetrahydroberberine drug eutectic prepared by the invention inherits the pharmacological activity of tetrahydroberberine, its solubleness, dissolution rate, antibiosis bacillus coli and staphylococcus aureus activities are significantly improved in relative to the tetrahydroberberine, thereby saving drug dosage; the method is good for developing to be drug preparation, and the tetrahydroberberine can be widely applied to the medicine field.

The invention discloses a mannanase and the coding gene, a recombinant vector and a host cell comprising the gene, and expression of the recombinant vector in E.coli and production of the enzyme in yeast cells by using the recombinant vector. The present invention also provides a new wild type subspecies of Bacillus Megaterium, in particular, Bacillus Megaterium FB101.

The invention belongs to the field of biotechnology, and discloses a laccase gene as well as an encoded protein and an application thereof. The open reading frame sequence of the laccase gene fmb-L103 is shown in SEQ ID NO. 1. The amino acid sequence of the protein encoded by the laccase gene fmb-L103 disclosed by the invention is shown in SEQ ID NO. 2. The laccase gene fmb-L103 disclosed by the invention is applied to decoloration for triphenlmethane dyes. The inventor clones from the selected dead bacillus vallismortis fmb-L103 with a spore laccase activity to obtain a novel prokaryotic laccase gene fmb-L103, and realizes the heterologous efficient expression of escherichia colli expression host bacteria. The recombinase fmb-L103 can be used for efficiently catalyzing the degradation of the triphenlmethane dyes of malachite green, brilliant green and aniline blue.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

The invention discloses a mycobacterium tuberculosis TB10.4-F1 fusion protein vaccine and a preparation method thereof. The preparation method comprises the following steps of: connecting the mycobacterium tuberculosis TB10.4 protein gene with the F1 protein gene of respiratory syncytial virus protein, transferring into a colibacillus expressing strain, inducing by IPTG (isopropyl beta-D-1-thiogalactopyranoside) to express the TB10.4-F1 fusion protein, and acquiring the protein with higher purity by metal ion affinity chromatography. The fusion protein has stronger immunogenicity, can excite more balanced Th1/Th2 immune response, and can prevent infections of the mycobacterium tuberculosis and the respiratory syncytial virus.

The invention provides a textile material. The textile material is prepared by twisting the following components in percentage by mass: 62% to 70% of cotton fiber, 12% to 20% of bamboo charcoal fiber, 5% to 8% of chitin fiber, and 6% to 10% of acrylic. Since the bamboo charcoal fiber has a better dehumidifying effect, the chitin fiber has higher humidity absorbing, ventilating and deodorizing capabilities, and the common strains, such as colon bacillus, hay bacillus, staphylococcal aureus and lactobacillus, have better bacteriostatic functions, the textile material has the advantages of sweat absorbing, degerming, heat insulation, and comfortable wearing.

The invention relates to a method for preparing Ag2MoO4-ZnMoO4-CuMoO4 by microwave heating. A test proves that the chemical reaction of the antimicrobial can be promoted to finish under microwave heating condition, thereby greatly shortening the preparation time of the antimicrobial. And when three components of coprecipitation molybdate antimicrobial powder body solution are prepared, a coprecipitation way is adopted, so that the grain diameter of the antimicrobial is evener, and the antimicrobial effect is improved. Through analyzing and comparing the XRD of a baking sample which is not baked at 1260 DEG C, the three components of molybdate antimicrobial has fine stability at high temperature. And the compound of parts of CuMoO4 and ZnMoO4 substitutes the compound of Ag2MoO4, so that the cost of the antimicrobial is lowered. The prepared Ag2MoO4-ZnMoO4-CuMoO4 antimicrobial is added into sanitary porcelain according to the proportion that the dosage of the antimicrobial is 1 to 2wt percent of the gross weight of dried glaze, and is baked at about 1260 DEG C to obtain an antimicrobial ceramic sample. Through an antimicrobial performance test, the bacteriostatic ratios of antimicrobial ceramic to colon bacillus and staphylococcus aureus are both at over 90 percent which accords with the standard of the antimicrobial ceramic in the building material industry.

The invention discloses a colony, expression and appliance of lactic acid bacteria aminoglutaric acid decarboxylase gene in food industry biotechnics domain, which is characterized by the following: coming from spittle streptococcus thermophilic subspecies (Streptococcus thermophilus) with length at 1380bp; augmenting from gene group DNA through PCR; adding NcoI and EcoRI on the two ends of the gene; limiting enzyme identification sequence; connecting to pET-DsbA of same limited enzyme alimentary; transforming expressing host bacteria BL21(DE3)pLysS of bacillus coli; realizing retooling expression in bacillus coli; getting glutamic acid decarboxylase molecule with molecular weight at 52. 4kDa; transforming L- glutamic acid decarboxylase to gamma-aminobutyric acid with retooling enzyme; providing a great amount of and high active rough enzyme for enzymatical synthesis of gamma-aminobutyric acid; decreasing cost of enzymatical synthesis of gamma-aminobutyric acid. This method possesses warm condition, which belongs to biological synthesis method.

The invention relates to a complexly-formulated quaternary ammonium salt disinfectant. The complexly-formulated quaternary ammonium salt disinfectant is prepared from the following ingredients: N,N-bis(3-amino propyl) dodecylamine used as a main ingredient of the antibacterial agent, didecyldimethylammonium chloride (DDDMAC) with strong antibacterial effect, a synergist, a stabilizing agent, a pH regulator, an antioxidant and a cosolvent; the complexly-formulated quaternary ammonium salt disinfectant is mainly characterized in that novel, stable and high-efficiency quaternary ammonium salt is adopted for achieving disinfectant and antibacterial effects; the complexly-formulated quaternary ammonium salt disinfectant can rapidly kill fungus, saccharomycetes, colon bacillus, pseudomonas aeruginosa, Staphylococcus aureus, Tritirachium album, virus and other microorganisms in a highly-efficiency broad-spectrum manner; the complexly-formulated quaternary ammonium salt disinfectant has the advantages of having wide application range, good disinfection effect, no toxic action, no carcinogenic effect, no sensitization, no mutagenesis, no pesticide resistance formation, no irritation, no corrosivity, no foreign smell and no flammability, having capability of effectively killing harmful microorganisms such as bacteria, viruses and fungus, which are harm to higher organisms in all life stages (including a reproduction period and a juvenile stage), having capability of natural degradation in soil, no secondary pollution, having stable performance, having less possibility of losing efficacy due to light, heat and the like, and having economical and practical effects, can achieve rapid high-efficiency broad-spectrum disinfection effect compared with similar products, and has low price.

The invention provides a genetic chip and a detection kit related to major pathogenic microorganisms causing infectious diarrhea of human beings, which are mainly specific to 13 types of bacteria including lapactic bacillus coli/shigella, salmonella, comma bacillus, vibrio parahemolyticus, aeromona hydrophila, plesiomonas, virio hollisae, vibrio fluvialis, vibrio mimicus, Wauter's strains, vibriodamsela and vibrio furnissii. The genetic chip comprises a solid-phase carrier and an oligonucleotide probe fixed on the solid-phase carrier, wherein the oligonucleotide probe comprises DNA (Deoxyribonucleic Acid) fragments selected from genes including a gyrB gene, an ITS gene, a dnaJ gene, a toxR gene and the like with remarkable biological evolution advantages or complementary DNA fragments. By adopting the genetic chip and the detection kit provided by the invention, major pathogenic microorganisms causing infectious diarrhea of human beings can be detected. The genetic chip and the detection kit have the characteristics of easiness and convenience for operation, high flux, high accuracy, high repeatability and the like, and can be applied to clinical detection of inspection departments in hospitals.