492 results about "Bacillus coli" patented technology

The present invention relates to an active peptide feed addictive, as a mixture of one or more plant protein and animal protein, which is the product of the following steps: fermenting and degrading the mixture through bacillus and the like, controlling the degree of hydrolysis at 20-35%, obtaining a fermentation supernatant after a separation post-processing process, concentrating drying the supernatant or directly adsorbing the supernatant onto the carrier, and then low temperature drying it. Said active peptide feed addictive is capable of obviously depressing development of bacillus coli and salmonella, and can prevention and cure diarrhea caused by bacilli or lienteric diarrhea; can exert its functions at a very low concentration, with a very intense effect; can regulate stomach and intestine bacterium group and promote animals digestion functions; can excite animal immune function, reinforce animal disease resistance against a plurality of epidemic diseases and greatly reduce the morbidity and death rate; can promote animal development and improve the growth velocity; can improve the feed use ratio and reduce production costs. Applications of the microorganism fermentation degradation method provided by the present invention have a low fabricating cost, and the obtained peptides have many kinds and high content, without environment pollutions.

Bacillus strains that inhibit pathogenic swine E. coli and / or improve performance are provided. Inhibition of pathogenic swine E. coli decreases E. coli disease. At least one strain enhanced swine performance by improving average daily gain, feed efficiency, and feed intake. Preferred Bacillus strains are of species that are included on the GRAS list. Bacillus species are sporeformers and therefore are highly stable and can be fed to swine.

The invention provides a light high elasticity deodorant rubber spongy cushion for shoes and a preparation method thereof. The light high elasticity deodorant rubber spongy cushion comprises components based on parts by weight: 50-150 parts of natural rubber, 2-50 parts of cis-butadiene rubber, 2-3 parts of sulphur, 0.8-4.5 parts of vulcanization accelerator, 1.5-5 parts of sulfurization activator, 1.5-2.5 parts of foaming agent, 15-25 parts of stearic acid, 35-45 parts of filler and 1.5-3 parts of deodorant. The rubber spongy cushion has reasonable elasticity and light specific gravity, reduces the fatigue degree when being worn, is good in the effect of preventing funguses such as candida albicans, staphylococcus aureus, bacillus coli and the like as well as bacteria, and greatly improves the hygiene and the functionality of the rubber shoes; furthermore, the rubber spongy cushion prepared by the method has proper thickness, so as to improve the comfort feeling. In addition, the materials selected by the invention can be easily obtained, and the invention has the advantages of low cost, simple preparation method, easy implementation and good performance of the produced product.

The invention discloses a multi-epitope fusion diagnosis antigen for African swine fever virus as well as a preparation method and application thereof. An ASFV (African swine fever virus) important structural protein gene encoding amino acid sequence is analyzed, screened and recombined through bioinformatics software, a multi-epitope fusion antigen gene is built and synthesized and is expressed in bacillus coli; through screening, the recombinant multi-epitope fusion antigen ASFV-meAg6 is obtained, so that diagnosis antigen protein with strong specificity and high sensitivity is provided foran ASFV serological diagnosis method.

The invention provides a gene, recombinant plasmid and polypeptide for an anti-tumor binary polypeptide. The gene of the recombinant anti-tumor binary polypeptide is obtained by connecting in an operable way the gene of a coding antibody simulator with a recombinant bacillus anthraci protein antigen gene. The recombinant plasmid of the invention is formed by inserting the gene of the coding antibody simulator by double-chain oligomeric nucleotide directed mutagenesis method into the recombinant bacillus anthraci protein antigen gene. The obtained recombinant plasmid is infected into engineering bacillus coli BL-21 to get engineering bacillus coli cell of anti-tumor binary polypeptide; the anti-tumor binary polypeptide can be obtained by expanding the bacillus coli, settling in centrifugal way the bacillus coli body, crushing in altrasonic way, settling and crushing bacillus coli body by hi-speed centrifuging and treating the upper clean solution. The anti-tumor binary polypeptide is of special targeting characteristic, higher efficiency in killing special physical tumor than prior anti-tumor medicine, and will not attack normal cells, and has much lower toxicity and poor-reaction than prior anti-tumor medicine.

The present invention is diphtheria toxin mutant CRM197 and its preparation process, and belongs to the field of immune protein carrier technology. The diphtheria toxin mutant CRM197 is expressed in colibacillus in the form of inclusion body, and the target product has sequence length of 536 amino acids, the immunogenicity the same as that of diphtheria toxin and no toxicity similar to that of diphtheria toxin. The present invention clones gene coding CRM197, constructs recombinant expression plasmid expressing CRM197 and transforms to colibacillus host for expression in the form of inclusion body. The present invention has target protein accounting for over 24 % of total protein content, high CRM197 purity over 95 % and high target protein recovering rate. The preparation process is simple, low in cost and suitable for industrial production.

The invention discloses a long-chain recombination human bone pattern generating protein-2 and preparing method and application, which is characterized by the following: utilizing gene project technique to grow the protein in the expressing system of escherichia coli and bacillus subtilis; adopting human bone sarcoma cell mRNA to do reverse transcription to obtain the cDNA as form; augmenting nucleotide sequence of entire 114 amino acids naturally from carboxyl end; adding a segment of nucleotide in front of the first codon of primer 5' end; increasing a segment of polypeptide at N end corresponding to amino acid sequence; obtaining long-chain rhBMP-2 gene with molecular weight at 30KD and purity over 95%.

The invention belongs to biology technology field, which concretely refers to controlled release tissue factor path inhibitor (LTFPI) and the manufacturing method, and the application. The invention analyzes and experiments the biology information science and structure molecular biology science of tissue factor path inhibitor (TFPI) and its acceptor low density lipoprotein acceptor correspondent protein (LRP), it ascertains the part where the TFPI carboxy end combines with LRP and is eliminated. It designs TFPI carboxy end mutant, which is recombined with primary nucleus or eukarya expressing carrier after constructing LTFPI gene through PCR location, it converts bacillus coli or bici yeast, sifts the high expression project fungus. The primary project fungus are yeasted and expanded, crushed, then they are centrifugated and collects the inclusion body, purifies the LTFPI through molecular sift and ion interchanging two-step method; the eukarya project fungus are carried on with two-step purifying directly. The half-life are prolonged, it has good pour-depressant function.

The invention provides a polyhexamethylene guanidine propionate disinfectant and a preparation method thereof. The polyhexamethylene guanidine propionate disinfectant is a hydrophilic solution prepared by the following steps: adding polyhexamethylene guanidine propionate, citric acid and surfactant to water and uniformly stirring the mixture at normal temperature. The polyhexamethylene guanidine propionate disinfectant has the advantages of broad spectrum of disinfection, quick results, long-time disinfection capability, high safety, non-toxicity, environmental protection, high stability, easy application, quick infiltration and volatilization, no coloration, easy cleaning, and the like. The polyhexamethylene guanidine propionate disinfectant has strong sterilization functions for colon bacillus, staphylococcus aureus, candida albicans, and the like with two-year effective periods. The polyhexamethylene guanidine propionate disinfectant can be used for disinfection of skins, wound surfaces, hands, air, departments of gynecology and urology, drinking water, and the like, thereby being a novel and ideal disinfectant.

The present invention provides one kind of gene, recombinant plasmid and polypeptide of EB virus resisting tumor polypeptide. The coding inducing polypeptide gene and coliform group gene are connected via operation to obtain polynucleotide sequence expressing recombinant antitumor polypeptide. The nucleotide sequence of the coding inducing polypeptide is inserted into coliform group gene by means of double strand oligomeric nucleotide point mutation technology to form the said recombinant plasmid. The obtained recombinant plasmid is transfected into engineering colibacillus TG1 to obtain engineering bacillus cell capable of generating recombinant antitumor polypeptide; and through mass proliferation, centrifugal precipitation and other steps, recombinant antitumor polypeptide is obtained. The antitumor polypeptide has specific targeting property, high efficiency of killing solid tumor, no attack to health cell, low toxicity and less negative reaction.

The invention relates to a pig breeding and breathing complex virus (PPRSV) and pig 2-type loop virus (PCV 2) series gene recombination adenovirus and vaccine in the field of high and new biotechnology. It uses PCR technology to clone the Cap protein gene into the plasmid carrier pShuttle-CMV-GP5 by open type reading rule and transforms tobacillus coli BJ5183strain with cage carrier pAdEasy-1 to capture the recombination plasmid; it uses recombination plasmid HEK293-A cell to capture recombination adenovirus and purifies it to express the recombination adenovirus rAd-Cap-GP5 of PRRSV GP5protein and PCV-2 Cap protein.

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

A preparing method of a silver / titanium oxide nanotube antifouling agent interfacially modified by polydopamine is disclosed. The preparing method comprises following steps of: (1) preparing titanium oxide powder; (2) preparing titanium oxide nanotubes by adopting a hydrothermal method and by adopting the obtained titanium oxide powder as a reaction substrate; (3) modifying surfaces of the titanium oxide nanotubes by utilization of the polydopamine; (4) adding the obtained titanium oxide nanotubes the surfaces of which are modified with the polydopamine into a silver nitrate solution, and adsorbing silver ions by utilization of a vacuum adsorption method; (5) subjecting a precipitate product to centrifugal separation, and irradiating under ultraviolet light; and (6) adding the product irradiated by the ultraviolet light into the silver nitrate solution again, and repeating the step (4) and the step (5) for a plurality of times to finally obtain the antifouling agent. The preparing method is simple, feasible, mild in reaction conditions, low in equipment requirement, capable of large-scale production, and high in popularization and application value. The prepared antifouling agent has significant long-acting bacterial inhibiting effects for staphylococcus aureus, escherichia coli and vibrio anguillarum.

The invention discloses a method for detecting bacterial amount of gold nanoparticles by using a coated antibody. The method provided by the invention comprises the following steps: I, the gold nanoparticles which are coated with a specific antibody of bacteria to be detected are reacted with a sample, and thus reaction liquid is obtained; and II, a dark-field scattering image can be obtained by using a dark-field microscope to observe the reaction liquid obtained in the step I, and the amount of the bacteria to be detected can be obtained by analyzing the dark-field scattering image with an image analysis technology. According to the method disclosed by the invention, The test shows that since the gold nanoparticles which are modified by the antibody are used as a probe to carry out a dark-field scattering imaging, and an automation image identification technology is adopted; and therefore the method has the advantages of high sensitivity, strong specificity, fast detection speed and capability of being suitable for quick and sensitive detection of bacillus coli.

The invention discloses an isothermal nucleic acid amplification reaction reagent which comprises Tris buffer solutions, potassium acetate, magnesium acetate, dithiothreitol, polyethylene glycol, adenosine triphosphate (ATP), deoxy-ribonucleoside triphosphate (dNTPs), phosphocreatine, creatine kinase, a primer group, single-strand binding (SSD) protein, colon bacillus helicase RecQ protein, UvsY protein and DNA polymerase. The invention further discloses an isothermal nucleic acid amplification method which includes steps of extracting DNA or performing inverse transcription after DNA extracting, and adding the isothermal nucleic acid amplification reaction reagent and reacting the mixture at a temperature in a range between 25 DEG C and 45 DEG C for 10 minutes to 60 minutes to complete the nucleic acid amplification. Compared with traditional polymerase chain reaction (PCR) technologies and according to the technology, nearly no professional instrument is needed, the operation is simple, technical requirements for operators are low, and the reaction time only needs about half an hour.

The invention belongs to the technical field of microorganism genetic engineering and discloses separation and cloning of nematicidal crystal protein gene originated from thuringiensis and biological activity of the nematicidal crystal protein gene on nematode. In the invention, a new insecticidal crystal protein gene cry1518-35 is obtained by being separated from bacterial strain of activated thuringiensis YBT-1518 of root-knot nematodes in north of China; an encoded product of the gene is a new insecticidal crystal protein Cry1518-35; the protein Cry1518-35 features high insecticidal activity on root-knot nematodes in north of China. The invention also includes the preparation of colon bacillus recombinant bacteria EMB0225 which expresses the insecticidal crystal protein Cry1518-35, the recombinant colon bacillus is preserved in CCTCC, the preservation number is CCTCC NO: M209009.

One producing method of gene composition of pig a-interferon, expression vector structuring and its offspring includes synthesing pig a-interferon consisted of bacilluscoli preference codon and constructs highly active expression vector with this gene, and realizes the highly active expression in bacilluscoli of pig-a interferon, with 26% expression of the whole mycoprotein. At the same time, it provides a purely chemical engineering system of technique of induced expression, inclusion bodies separating and cracking, and complexity of albumen. Pig a-interferon has the functions of highly active antivirus and immunoregulation, and can be applied in preventing and treatment of pig contagion. This invention is able to produce efficient, safe, and cheap pig a-interferon which has no potential virus pollution. This lays a solid foundation for mass-production of pig a-interferon in our country.

A feed additive containing yolk antibodies against diarrhea in pigs. The antibodies are preparing from bacilluscoli, epidemic diarrhea or rotavirus, or immunizing non-immunitive hens laying eggs withcombined vaccine of bacilluscoli and / or epidemic diarrhea and / or rotavirus, then collecting yolks of the hens. A yolk antibody injecting agent, which is prepared from bacilluscoli, epidemic diarrheaor rotavirus, or immunizing non-immunitive hens laying eggs with combined vaccine of bacilluscoli and / or epidemic diarrhea and / or rotavirus, then collecting yolks of the hens.

Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.

The invention discloses a phosphate tetrahydroberberine drug eutectic and a preparation method thereof. The tetrahydroberberine and phosphate are added and mixed in isopropanol water solution, ethanol water solution or methanol water solution according to the molar ratio; the acquired mixed solution is volatilized under the room temperature. The prepared tetrahydroberberine drug eutectic is a triclinic system, and the space group is P-1, and its axial length (i) is FORMULA; the axial angle is FORMULA. The tetrahydroberberine drug eutectic prepared by the invention inherits the pharmacological activity of tetrahydroberberine, its solubleness, dissolution rate, antibiosis bacillus coli and staphylococcus aureus activities are significantly improved in relative to the tetrahydroberberine, thereby saving drug dosage; the method is good for developing to be drug preparation, and the tetrahydroberberine can be widely applied to the medicine field.

The invention discloses a mannanase and the coding gene, a recombinant vector and a host cell comprising the gene, and expression of the recombinant vector in E.coli and production of the enzyme in yeast cells by using the recombinant vector. The present invention also provides a new wild type subspecies of Bacillus Megaterium, in particular, Bacillus Megaterium FB101.

The invention discloses a bentonite-loaded nano silver antibacterial agent and a preparation method thereof. The preparation method comprises the following steps: carrying out acid treatment on bentonite to obtain acidification-modified bentonite; putting the acidification-modified bentonite into a dispersion stabilizer sodium polyphosphate water solution; adding a silver-ammonia solution and carrying out an illumination reaction to insert compound powder composed of nano silver and silver ammino ions into a bentonite sheet layer, so as to obtain the bentonite-loaded nano silver antibacterial agent. The grain diameter of the antibacterial agent compound powder prepared by the invention is 0.1-5 microns and the silver content is 0.1-15wt%; a bacteriostasis circle to colon bacillus and staphylococcus aureus is more than or equal to 10mm and the minimal inhibitory concentration is 10-200 micrograms / milliliter; the bentonite-loaded nano silver antibacterial agent is suitable for being used for preparing antibacterial plastic, antibacterial textiles, domestic chemical antibacterial materials and the like.

The invention belongs to the field of biotechnology, and discloses a laccase gene as well as an encoded protein and an application thereof. The open reading frame sequence of the laccase gene fmb-L103 is shown in SEQ ID NO. 1. The amino acid sequence of the protein encoded by the laccase gene fmb-L103 disclosed by the invention is shown in SEQ ID NO. 2. The laccase gene fmb-L103 disclosed by the invention is applied to decoloration for triphenlmethane dyes. The inventor clones from the selected dead bacillus vallismortis fmb-L103 with a spore laccase activity to obtain a novel prokaryotic laccase gene fmb-L103, and realizes the heterologous efficient expression of escherichia colli expression host bacteria. The recombinase fmb-L103 can be used for efficiently catalyzing the degradation of the triphenlmethane dyes of malachite green, brilliant green and aniline blue.

The invention relates to anti-tubercule bacillus recombination protein. It belongs to gene recombination technique field. Its manufacturing includes the following steps: combining antigen the 190-198 polypeptides of the Ag85B-Mpt64 with Mtb8.4; cloning into escherichia coli vector to express; further purifying to gain. It is recorded as Ag85B-Mpt64190-198-Mtb8.4(SEQ.ID.NO.1). The protein can be used as anti tubercule bacillus subunit vaccine.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

The invention discloses a mycobacterium tuberculosis TB10.4-F1 fusion protein vaccine and a preparation method thereof. The preparation method comprises the following steps of: connecting the mycobacterium tuberculosis TB10.4 protein gene with the F1 protein gene of respiratory syncytial virus protein, transferring into a colibacillus expressing strain, inducing by IPTG (isopropyl beta-D-1-thiogalactopyranoside) to express the TB10.4-F1 fusion protein, and acquiring the protein with higher purity by metal ion affinity chromatography. The fusion protein has stronger immunogenicity, can excite more balanced Th1 / Th2 immune response, and can prevent infections of the mycobacterium tuberculosis and the respiratory syncytial virus.

The invention provides a textile material. The textile material is prepared by twisting the following components in percentage by mass: 62% to 70% of cotton fiber, 12% to 20% of bamboo charcoal fiber, 5% to 8% of chitin fiber, and 6% to 10% of acrylic. Since the bamboo charcoal fiber has a better dehumidifying effect, the chitin fiber has higher humidity absorbing, ventilating and deodorizing capabilities, and the common strains, such as colon bacillus, hay bacillus, staphylococcal aureus and lactobacillus, have better bacteriostatic functions, the textile material has the advantages of sweat absorbing, degerming, heat insulation, and comfortable wearing.

It relates to a bactericide, comprising lysostaphin 0.0001í½0.1wtúÑú¼lysozyme 10í½50wtúÑú¼stabilizer 1í½15wtúÑú¼surfactant 0.1í½0.5wtúÑú¼water. It also relates to an antiseptic wet towel, its preparation method and applications in treatment and prevention of mammary gland infection of milch cow. The bactericide can be carried by a carrier, then an antiseptic wet towel is obtained. The antiseptic wet towel could kill all bacteria causing mammary gland infection of milch cow. The killing rate for bacillus coli, staphylococcus aureus and the like, is above 99%.