76 results about "Bacillus coli" patented technology

The invention relates to a development method of an insect culture medium, which comprises the following steps: S1, constructing a double-promoter tandem vector by taking pFastBac plasmid as a skeleton; s2, establishing a baculovirus expression system; S21, transforming the double-promoter tandem vector into DH10Bac escherichia coli, so as to obtain recombinant Bacmid; s22, transfecting an Sf9 cell by using the recombinant Bacmid, so as to obtain a P0-generation baculovirus; s23, transfecting an Sf9 cell by using the P0-generation baculovirus to obtain a P1-generation baculovirus; s3, screening insect culture media: S31, providing culture media to be detected, and respectively inoculating Sf9 cells into the culture media to be detected; s32, respectively inoculating the P0-generation baculovirus or the P1-generation baculovirus into each to-be-detected culture medium for culturing; and S33, after culture is finished, detecting virus antibodies in the culture media to be detected, and screening the culture media. A comprehensive and quantitative evaluation system is established for development of the insect culture medium.

The invention belongs to the technical field of building materials, and particularly relates to a preparation method of alkali-treated polypropylene fiber and recycled aggregate loaded bacillus coriolis synergistic microbial concrete. According to the method, a polypropylene fiber reinforced concrete matrix is subjected to alkali treatment, calcium carbonate is generated by combining the mineralization effect of recycled aggregate loaded microorganisms, and efficient crack repair and mechanical property improvement are achieved. The method specifically comprises the steps of microbiological treatment and loading, fiber surface modification and optimization, self-healing concrete preparation and the like. The recycled aggregate is used for fixing microorganisms and providing a mineralization environment, the interface bonding capacity is enhanced through alkali treatment fibers, and the calcium carbonate deposition efficiency is remarkably improved. The prepared self-healing concrete has excellent mechanical strength and compactness, and the repairing performance is also obviously improved. The method is simple in process and controllable in cost, the utilization of the recycled aggregate conforms to the concept of green building materials, and the method can be widely applied to preparation of microbial concrete and has remarkable environmental protection value and application prospects.

The invention belongs to the field of biotechnology and environmental governance, and particularly relates to a manganese peroxidase mutant based on high-throughput screening modification and application of the manganese peroxidase mutant to straw degradation. Aiming at the problems of low natural secretion amount, insufficient specific activity, complex preparation process and the like of the existing manganese peroxidase, the method comprises the following steps: firstly, constructing engineering bacteria based on an escherichia coli exocrine system, constructing a mutant library through error-prone PCR in combination with a directed evolution technology, and performing high-throughput screening by utilizing an ABTS colorimetric method; the recombinant manganese peroxidase mutant with high catalytic activity is obtained, and the enzyme activity is improved by 1.29 times compared with that of a wild type. Furthermore, recombinant engineering bacteria are constructed on the basis of a bacillus subtilis exocrine system, and efficient secretion of the mutant enzyme is achieved. The mutant enzyme preparation is used for wheat straw degradation, and the lignin degradation rate reaches 41.37% (improved by 30.09% compared with that of a wild type). The method is simple and convenient in process, low in cost and remarkable in effect, and has a wide industrial application prospect.

The invention belongs to the technical field of gene engineering, and in particular relates to a D-psicose-3-epimerase mutant subjected to Spytag / SpyCatcher cyclization modification and an application of the D-psicose-3-epimerase mutant. The preparation method comprises the following steps: carrying out multi-site simultaneous mutation on 109th aspartic acid and 160th serine of wild type D-psicose-3-epimerase to obtain a mutation intermediate, respectively connecting a Spytag tag and a SpyCatcher tag to the N end and the C end of the mutation intermediate, and carrying out cyclization modification, so as to obtain the wild type D-psicose-3-epimerase, wherein the Spytag tag and the SpyCatcher tag are respectively connected to the N end and the C end of the mutation intermediate; when fructose is used as a raw material to produce D-psicose and host bacteria are escherichia coli, the reaction of catalyzing 600 g / L fructose to produce D-psicose is performed for 4 h, and the conversion rate can reach 35.7%; when the host bacteria are bacillus subtilis, the reaction for catalyzing 500 g / L fructose to produce D-psicose is performed for 4 h, and the conversion rate reaches 36.5%; compared with a wild type D-psicose-3-epimerase strain of bacillus subtilis, the conversion rate of the strain is improved by 13.5%.

The application discloses a method for improving the fermentation efficiency of soy sauce pool type fermentation, which is characterized by the following steps: using the self-source microorganism in the fermentation tank to carry out fermentation outside the fermentation tank, combining with the fermentation liquid of Lactobacillus reuteri for synergistic fermentation, and then returning the fermentation liquid to the fermentation tank for pouring and utilization, so as to realize the promotion of the self-source microorganism in the fermentation tank to the soy sauce fermentation. The self-source microorganism is fermented and cultured by reducing the salinity outside the fermentation tank and adding a proper amount of starch raw material, and the fermentation liquid with high activity of protease can be prepared in a short time, which is returned to the fermentation tank for pouring and utilization, so as to improve the degree and efficiency of protein hydrolysis in the fermentation tank. The Lactobacillus reuteri uses the fermentation liquid of the soy sauce mash in the fermentation tank as the basic nutrient source and ferments together with a proper amount of white granulated sugar to produce the fermentation liquid with the function of inhibiting Escherichia coli, so as to resist the Escherichia coli pollution in the fermentation process of the self-source microorganism outside the fermentation tank, guarantee the safety of the returned pouring fermentation liquid, and further improve the production efficiency of the soy sauce pool type fermentation technology.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

The present invention relates to a composition that is used to primarily disrupt and remove biofilm from surfaces. This is achieved using a composition comprising a bacteriophage capable of lysing bacteria selected from one or more of Pseudomonas aeruginosa, Acinetobacter baumanii, Staphylococcus aureus, and E.coli; and a Bacillus bacterial spore in the presence of a specific surfactant of the poloxamer type.

The invention belongs to the technical field of membrane separation, and particularly relates to a quercetin-doped reverse osmosis composite membrane, a preparation method thereof and a water purification method. The quercetin-doped reverse osmosis composite membrane comprises a polysulfone microfiltration base membrane and a quercetin-doped polyamide selection layer, the quercetin-doped polyamide selection layer is attached to the surface of at least one side of the polysulfone microfiltration base membrane; the quercetin-doped polyamide selection layer comprises quercetin and a polyamide skeleton, and the quercetin and the polyamide skeleton are connected through an ester bond and / or a hydrogen bond. The quercetin-doped reverse osmosis composite membrane at least meets one of the following conditions: the surface roughness is 121 nm to 199 nm; the water contact angle is 50-60 degrees; under the conditions of 20 bar and 25 DEG C + / -1 DEG C, the retention rate on a 2000 mg.L <-1 > NaCl solution is greater than or equal to 95%; the Zeta potential is-30mV to-60mV when the pH value is equal to 7; the sustained release rate of quercetin is 0.1 [mu] g.cm <-2 >. D <-1 >-10 [mu] g.cm <-2 >. D <-1 >; the killing rate of escherichia coli is greater than or equal to 90%; and the killing rate of staphylococcus aureus is greater than or equal to 90%.

The present invention relates to a recombinant bacterium producing Arctic-derived β-glucosidase and its application, belonging to the field of biotechnology. The target gene of the recombinant bacterium is β-glucosidase derived from Arctic bacteria Arc12, the nucleotide sequence of which is shown in SEQ ID No: 1, and the nucleotide sequence of the β-glucosidase with signal peptide is shown in SEQ ID No: 2, the signal peptide is Bacillus subtilis signal peptide CitH, and the expression vector is Escherichia coli-Bacillus shuttle plasmid pMA5, which contains constitutive promoter P HpaII The expression strain is Bacillus subtilis WB600. The present invention provides a method for secretory expression of the enzyme under specific promoter, signal peptide, and strain conditions. This method is simple to operate and can be achieved without the need for special inducers. Secretory expression of the enzyme eliminates the need for cell disruption in downstream processing, reducing production costs and possessing promising application prospects.

The invention relates to a method for extracting roselle anthocyanin through enzymolysis-ultrasonic assistance, a product and application of the product. The method comprises the following steps: degreasing roselle powder, and performing enzymolysis for 24 hours by using cellulase and pectinase (20U / g respectively) under the conditions that the pH is 5.0 and the temperature is 40 DEG C; an acetone-hydrochloric acid mixed solution (the volume fraction of hydrochloric acid is 36%) with the liquid-material ratio being 10: 1 serves as an extracting agent, and ultrasonic extraction is conducted for 34 minutes at the temperature of 58 DEG C and the power of 160 W; and finally, centrifuging, extracting with ethyl acetate, and purifying to obtain an anthocyanin product. According to the method, the extraction rate reaches 3.087 mg / g, the main component of the product is cyanidin-3-glucoside, the antioxidant and antibacterial activity is remarkable, the scavenging rates of DPPH, ABTS and hydroxyl radicals are 86.26%, 81.34% and 93.75% respectively under the concentration of 3.0 mg / mL, and the scavenging rates are superior to those of vitamin C with the same concentration; under the concentration of 11mg / mL, the compound has an obvious inhibition effect on escherichia coli and staphylococcus aureus. The process is efficient and mild, the product activity is excellent, and the method is suitable for development and application of natural antioxidants and antibacterial agents.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

The application discloses an L-aspartate alpha-decarboxylase with improved substrate tolerance, and the L-aspartate alpha-decarboxylase is derived from Bacillus subtilis and is subjected to site-directed mutation, i.e., phenylalanine at the 4th site is changed into tryptophan, isoleucine at the 33rd site is changed into alanine, and isoleucine at the 88th site is changed into tryptophan, so as to obtain an enzyme mutant; the mutant is recombined into E. coli, and after expression is induced, the mutant catalyzes the substrate L-aspartate to generate beta-alanine. When the substrate addition concentration of the whole cell catalytic system is 60 g / L, the conversion rates of the three enzyme mutants T4W, I33A and I88M can reach about 90%, and the yields are 1.2, 1.3 and 1.2 times of that of the unmutated strain respectively, and the substrate tolerance is obviously improved; the combined mutation also has a certain improvement on the substrate tolerance, and the finding has important research value for industrial preparation of beta-alanine.

The present application belongs to the technical field of biological agents, and discloses a compound preparation containing bacillus velezensis and piper betel for preventing and treating avian colibacillosis and application. The mass ratio of the bacillus velezensis complex bacteria and the piper betel extract is 2-4:0.02-0.03; the bacillus velezensis is named as BV-838 and BV-839. It is proved by tests that the bacillus velezensis and the piper betel compound preparation have potential interaction, can cooperate with each other, play synergistic effect, can effectively prevent and treat avian colibacillosis, have the effect of enhancing immunity and promoting growth, are novel, efficient, green and environmentally friendly, and have important significance for the development of non-antibiotic breeding industrialization.

Colistin is an important last-resort antibiotic, but its production involves significant challenges due to the presence of impurities resulting from various analogs and the limited number of native producers. In response, disclosed are mutant Paenibacillus bacteria that produce Colicin A more efficiently and / or Colicin A that is of higher purity and / or has a cleaner metabolite background. The disclosed mutant bacteria are genetically engineered to inactivate expression of one or more biosynthetic gene clusters (BGCs), increase expression of one or more other BCGs, and / or increase expression of one or more L-DABA pathway genes.

The present disclosure provides, among other things, methods for removing linear ribonucleic acid molecules from a sample by contacting the sample with ribonuclease R (RNase R) from a thermophilic organism, for example, Geobacillus stearothermophilus, Fervidobacterium pennivorans or Thermus thermophilus expressed and purified in E.coli. Also provided herein are methods of producing circular RNA and removing uncircularized linear RNA molecules, linear RNA byproducts, or linear RNA side products using an RNase R expressed or purified from a thermophilic organism. The present disclosure also provides methods of purifying RNase R from a thermophilic organism in E. coll, wherein the method comprises heat treatment at high temperatures, and compositions and kits comprising the same.

The present application relates to the field of genetic engineering and biological enzyme depolymerization of PET plastics, and particularly relates to a hydrophilic PET depolymerase mutant and application thereof. The present application obtains a hydrophilic PET depolymerase mutant V68R, or A137R or I243R by mutating the 68th, or 137th, or 243th amino acid residue of the PETase protein amino acid sequence into R. Compared with the wild-type PETase protein, under the premise of ensuring the depolymerization efficiency of PET / BHET and effectively depolymerizing PET / BHET, the expression level of soluble protein is improved, and the formation of inclusion bodies is reduced in the expression system of Escherichia coli, Bacillus subtilis or Pichia pastoris, which has economic feasibility, PETase high-yield universality, and wide application prospect.

The invention provides an antibacterial postoperative wound regeneration hydrogel preparation capable of promoting epithelial regeneration and a preparation method of the antibacterial postoperative wound regeneration hydrogel preparation. The preparation is prepared from chitosan quaternary ammonium salt, collagen, an antibacterial compound solution, an angiogenesis and epidermal repair active carrier, a cross-linking agent and a solvent, and the cross-linking agent is genipin or ethylene glycol diglycidyl ether. Vascular endothelial growth factors are encapsulated by liposome, epidermal growth factors are loaded in mesoporous silica microspheres, and sustained release of active ingredients is realized. In the preparation process, uniform dispersion and stable cross-linking of all the components are ensured through solution premixing, antibacterial compound dispersion, active carrier loading and ultrasonic-assisted cross-linking forming. The hydrogel can remarkably shorten the wound healing time and improve the neovascularization density and epithelium coverage rate, has an excellent antibacterial effect on staphylococcus aureus, escherichia coli and the like, and has a wide application prospect in the field of medicine preparation for promoting postoperative wound repair.

This invention belongs to the field of genetic engineering technology and discloses a high-efficiency expression method for recombinant collagenase and its application in the preparation of bovine bone collagen peptides. The collagenase gene derived from Bacillus sp. HUB-I-004 strain (accession number J9CTX3) was optimized using E. coli-preferred codons. A recombinant expression vector was constructed and transformed into E. coli BL21(DE3). After optimization of induction conditions and Ni-NTA affinity purification, a highly active recombinant collagenase with a specific activity of 231.05 U / mg was obtained. Bovine bone collagen was extracted using a high-temperature, high-pressure method. Bovine bone collagen peptides were prepared under optimal conditions of a substrate concentration of 10 mg / mL, an enzyme addition of 20%, and enzymatic hydrolysis at 37℃ for 8 h, significantly improving peptide yield. The obtained product is mainly composed of small molecule peptides and exhibits significant osteoblast proliferation-promoting activity, making it widely applicable in the preparation of health foods and pharmaceuticals.

The present invention discloses an Aspergillus oryzae JAAS-32 and use thereof in preparation of a larval Hermetia illucens paste protein peptide. The Aspergillus oryzae JAAS-32 was deposited in Guangdong Microbial Culture Collection Center (GDMCC) on Aug. 10, 2023 with an accession number of GDMCC No: 63725, classified and named Aspergillus oryzae. A larval Hermetia illucens paste is subjected to enzymatic fermentation by the Aspergillus oryzae JAAS-32 provided by the present invention for 20-40 hours. The content of the protein peptide (<10 kDa) is 1.53 times that of an untreated group; the content of free amino acids is 1.41 times that of the untreated group; the total antioxidant activity is 1.35 times that of the untreated group. Diameters of inhibition zones against Staphylococcus aureus, Escherichia coli, and Vibrio alginolyticus are 1.62, 1.17, and 1.45 times those of the untreated group, respectively. The strain is cultured readily, fast in growth, and well-adapted. A low-cost, easy-to-operate, and efficient method for enhancing antibacterial performance of the larval Hermetia illucens paste can be established on the basis of the strain.

The invention discloses a method for crystallizing BM3 protein in bacillus megaterium. The method comprises the following steps: constructing a gene for coding the BM3 protein into an expression vector, carrying out induced expression in escherichia coli, carrying out affinity chromatography purification, excising a fusion tag, carrying out gel filtration purification, incubating with cofactors FAD, FMN and NADPH to form a compound, and crystallizing under a specific pool liquid condition through a seated drop gas phase diffusion method. According to the method, the high-quality BM3 protein crystal suitable for X-ray diffraction analysis can be obtained, and a basis is provided for analyzing the three-dimensional structure of the BM3 protein crystal and researching an electron transfer mechanism.

The invention discloses a bacillus subtilis genome integrated plasmid for efficient and stable expression of genes and application of the bacillus subtilis genome integrated plasmid in production of beta-galactosidase. The plasmid comprises a bacillus subtilis promoter, an RBS sequence, a terminator, a bacillus subtilis resistance gene, a genome integration homologous arm, an escherichia coli replicon and a resistance gene. The homologous arm is selected from upstream and downstream sequences of sites behind termination codons such as genes spxA, tatCY, rlmCD and the like, so that exogenous genes are accurately integrated to a plurality of non-essential sites on the premise of not damaging essential genes. The invention also provides a method for constructing a bacillus subtilis engineering strain for producing beta-galactosidase by using the plasmid. The integration system is high in expression level, stable expression can be achieved without adding antibiotics and inducers, the fermentation cost is remarkably reduced, the enzyme production efficiency is improved, and the integration system is suitable for industrial enzyme preparation production.

The present invention provides compounds of the general formula (I) or a pharmaceutically acceptable prodrugs, salts and / or solvates thereof, wherein LHS is selected from the group consisting of LHSa and LHSb And wherein, the asterisk (*) marks the point of attachment; These compounds exhibit antibacterial activity against Gram-negative and Gram-positive bacteria, especially S. aureus, E. coli, K. pneumoniae and A. baumannii. Pharmaceutical compositions containing these compounds, therapeutic uses thereof and methods for manufacturing the same are also provided.

The invention refers to a composition comprising inactivated cells deficient in LPS from the genus Acinetobacter and / or outer membrane vesicles form the same and their use for the manufacture of a medicament, preferably a vaccine, for the prevention of diseases caused by K. pneumoniae, P. aeruginosa, E. coli, and / or A. pleuropneumoniae, and optionally A. baumannii.

The invention discloses preparation of a mulberry leaf peptide and gallic acid synergistic antibacterial compound and application of the mulberry leaf peptide and gallic acid synergistic antibacterial compound in bacteriostasis and preservation, and belongs to the technical field of food preservation. The antibacterial compound comprises mulberry leaf peptide and gallic acid, and the mass ratio of the mulberry leaf peptide to the gallic acid is 5: (1-3). The preparation method of the antibacterial compound comprises the following steps: (1) obtaining mulberry leaf protein; (2) carrying out mixed fermentation on the mulberry leaf protein in a liquid medium by utilizing lactobacillus rhamnosus and lactobacillus reuteri to obtain mulberry leaf peptide; and (3) reacting the mulberry leaf peptide with gallic acid in any one of non-covalent binding, alkaline catalytic covalent binding or free radical grafting initiated covalent binding to prepare the gallic acid-mulberry leaf peptide compound. The compound has an antibacterial function of effectively inhibiting staphylococcus aureus and escherichia coli, and can remarkably improve the fresh-keeping effect of fruits, thereby laying a foundation for developing an innovative, safe and efficient natural fresh-keeping agent in the food industry.

The invention belongs to the technical field of aquatic product pathogen gene editing, and particularly relates to a target gene knockout method for photobacterium damsela subsp. Piscicida (PDP), which is characterized in that the target gene knockout method for the photobacterium damsela subsp. Piscicida (PDP) is disclosed. The method comprises the following steps: obtaining a DNA fusion fragment with deletion of a target region fragment of a target gene (MshL) by adopting overlapping PCR (Polymerase Chain Reaction), constructing a recombinant vector by using the obtained DNA fusion fragment, and obtaining the target gene knocked-out photobacterium damnieri subspecies fish by taking escherichia coli S17-1 lambda pir as a donor bacterium of a recombinant plasmid and PDP as a recipient bacterium. Specific knockout of the PDP specific gene is achieved for the first time, the knockout efficiency is high (the single-exchange positive rate is 29.2%, and the double-exchange positive rate is 30.8%), the cost is low, and the method is suitable for construction of a PDP specific gene deleted strain and functional research of the specific gene.

Colistin is an important last-resort antibiotic, but its production involves significant challenges due to the presence of impurities resulting from various analogs and the limited number of native producers. In response, disclosed are mutant Paenibacillus bacteria that produce Colicin A more efficiently and / or Colicin A that is of higher purity and / or has a cleaner metabolite background. The disclosed mutant bacteria are genetically engineered to inactivate expression of one or more biosynthetic gene clusters (BGCs), increase expression of one or more other BCGs, and / or increase expression of one or more L-DABA pathway genes.

The invention relates to a Juncao-sourced natural antibacterial, antiviral and environment-friendly clothing lining as well as a preparation method and application thereof. The lining of the garment is woven and formed in a weaving or knitting mode by taking Juncao regenerated cellulose fiber filaments as a core functional material; wherein the weaving mode comprises that warp and weft yarns adopt a full-fungus grass filament framework A of fungus grass regenerated cellulose fiber filaments, or warp yarns adopt common polyester fiber filaments / common viscose fiber filaments, and weft yarns adopt a composite synergistic framework B of fungus grass regenerated cellulose fiber filaments. The functions of the Juncao regenerated cellulose fiber filaments can be enhanced by combining with a nano-scale metal oxide finishing agent. The preparation method comprises the following green after-finishing processes: preparing the fiber filaments by a green and environment-friendly method, weaving and forming, and carrying out biological enzyme desizing, biological polishing, low-temperature setting and the like. The product has excellent antibacterial and antiviral properties (for example, the 24-hour bacteriostasis rate for staphylococcus aureus, escherichia coli and candida albicans is greater than or equal to 95%, and the activity rate for resisting H3N2 and H1N1 avian influenza viruses is greater than or equal to 90%), has the characteristics of high comfort and high biodegradation rate, and is suitable for linings of high-end clothes such as business suits, overcoats, down jackets and the like. Unification of functions, comfort and environmental protection is achieved, and the good industrialization prospect is achieved.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.