26 results about "Alcaligenes" patented technology

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

The present application belongs to the technical field of sewage treatment, and particularly relates to a method for simultaneously improving the denitrification efficiency of sewage and degrading new pollutants. Alcaligenes The present application adopts a new strain of Alcaligenes sp. FDN-09 which is resistant to perfluorinated compounds to carry out large-scale culture, and prepares a high-efficiency functional bacterial agent; under the condition that ammonia nitrogen and new pollutants coexist in sewage, the high-efficiency functional bacterial agent is added in a biological reactor, and the reactor is operated under anoxic and low-oxygen conditions, thereby simultaneously strengthening the removal of ammonia nitrogen and new pollutants in the sewage, and achieving the effect of improving the denitrification of the sewage treatment system while degrading new pollutants. The method of the present application has no secondary pollution to the environment, has high simultaneous degradation rates of total nitrogen and new pollutants, low treatment cost, and low carbon emission. Under the condition that ammonia nitrogen and new pollutants coexist in the sewage, the water quality of the effluent is far lower than the first-level A effluent standard in the modification single of the Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB 18918-2002).

The invention discloses ammonia oxidizing bacteria and application thereof, relates to the technical field of microorganisms, in particular to ammonia oxidizing alcaligenes and a symbiotic combination of the ammonia oxidizing alcaligenes and Pseudomonas oleovorans, and the ammonia oxidizing alcaligenes and the Pseudomonas oleovorans have the advantages of being high in biofilm culturing efficiency, short in starting time and high in nitrification efficiency.

The present application provides a nitrilase mutant and its application. Compared with a wild-type nitrilase with an amino acid sequence shown in SEQ ID NO: 2, the nitrilase mutant comprises one or more of the following amino acid mutation sites: S201F, G239M, K242T, V243Q and G246Q. The present application constructs a nitrilase mutant with high enzyme activity, high salt tolerance and high substrate tolerance by mutating and modifying a nitrilase derived from Alcaligenes.

The invention discloses a preparation, separation and purification method of glucan oligosaccharide, and belongs to the technical field of active oligosaccharide preparation. The preparation, separation and purification method comprises the following steps: adding alcaligenes faecalis into a fermentation culture medium, fermenting to prepare beta-1, 3-glucan, adding beta-1, 3-glucanase when the viscosity of fermentation liquor reaches a certain value, and simultaneously controlling the temperature to promote enzymolysis, so that the beta-1, 3-glucan with high polymerization degree is hydrolyzed into oligosaccharide, and separating and purifying the oligosaccharide. The material liquid viscosity is reduced, and the mass transfer is improved, so that the single-batch fermentation yield is improved. Meanwhile, sugars with different polymerization degrees are separated through a graded alcohol precipitation process, part of impurities are removed, and the product purity is improved. According to the method, the enzymolysis reaction is directly carried out in the fermentation process, so that the steps of polysaccharide extraction and enzymolysis are simplified, the production energy consumption is reduced, and the yield and the production benefit of the final product are improved.

The invention relates to the technical field of sewage treatment, and provides a magnetic biochar catalyst and a preparation method and application thereof in degradation of antibiotic wastewater, and the magnetic biochar catalyst is prepared from the following raw materials in parts by weight: 4-6 parts of bagasse, 4-6 parts of pomelo peel, 4-6 parts of orange peel, 5-7 parts of soluble ferric salt, 5-7 parts of soluble manganese salt, 13-17 parts of chitosan, 4-6 parts of bacillus cereus powder and 4-6 parts of alcaligenes powder. According to the application, biochar formed by pyrolysis of agricultural wastes such as bagasse and chitosan cooperate with adsorption, tetracycline in wastewater is rapidly enriched, the local concentration is increased, and the toxicity of tetracycline to microorganisms is reduced; the strain can reduce the toxicity of pollutants and improve the utilization efficiency of a chemical oxidant; the soluble metal salt activates ozone and persulfate to deeply oxidize tetracycline. It can be seen that the magnetic biochar catalyst achieves efficient removal of antibiotic pollutants through the synergistic effect of physical adsorption, biodegradation and chemical oxidation.

The invention provides a carbon sink type tailing soil conditioner as well as a preparation method and a soil treatment method thereof. The invention relates to a carbon sink type tailing soil conditioner, which comprises porous biochar loaded with an alkali-producing microbial agent and a multifunctional composite microbial agent with heavy metal toxicity reduction and efficient carbon sequestration effects; the alcaligenes microbial agent comprises alcaligenes; the multifunctional complex microbial inoculant comprises heavy metal passivating bacteria, carbonic anhydrase secreting bacteria and mycorrhizal fungi; the mycorrhizal fungi comprise at least one of arbuscular mycorrhizal fungi and ectotrophic mycorrhizal fungi; the porous biochar is prepared from the following raw materials: sodium carbonate and organic waste powder. According to the method, the rapid weathering of the tailings, the reconstruction of the soil structure and the efficient storage of CO2 are realized, the soil period is shortened from more than 5 years to 8-12 months, the carbon sink strength is improved to 2.1-3.4 t CO2 / ha.a, and an innovative solution is provided for the goals of ecological restoration and carbon neutralization of mines.

This invention discloses a strain of *Alcaligenes* HHR-1-1 and its application in the degradation of carotenoids and the preparation of flavorings, relating to the field of microbial fermentation technology. The *Alcaligenes* strain of this invention (… Pseudomonas alcaligenes The HHR-1-1 strain has the accession number CCTCC NO: M 20252745, and the *Alcaligenes* HHR-1-1 strain was deposited at the China Center for Type Culture Collection on December 1, 2025. The *Alcaligenes* HHR-1-1 strain can degrade carotenoids with a degradation rate of 80%–97%, and its main degradation products are β-ionone and its oxides, and dihydroactinolone. Furthermore, the fermentation broth of *Alcaligenes* HHR-1-1 strain and carotenoids can be used in flavor preparation, effectively improving the quality of the flavors.

The invention discloses an alcaligenes 09 and application thereof, the alcaligenes 09 is preserved in China Center for Type Culture Collection with the preservation number of CCTCC M 2025997, the 16S rDNA sequence of the alcaligenes 09 is shown as SEQ ID NO.1, and the alcaligenes 09 has a specific form and physiological and biochemical characteristics. The invention also discloses a liquid inoculant containing the strain and a preparation method of the liquid inoculant. The liquid inoculant is prepared through specific culture, fermentation and dilution steps. The degradation rate of the strain to 50 mg / L of albendazole within 72 h reaches 82.65%, the maximum tolerance concentration to cadmium is 80 mg / L, and the strain can be used for repairing water or soil co-polluted by albendazole and cadmium, has the advantages of high efficiency, environmental protection and low cost, and has important application value in environmental pollution treatment.

The invention belongs to the field of tumor immunity, discloses application of a pseudomonas alcaligenes composition in preparation of pancreatic cancer treatment drugs, and aims to solve the technical problems that pancreatic cancer immunotherapy is low in response rate and existing treatment schemes are high in toxicity. The pseudomonas alcaligenes used in the invention are sPseudomonas alcaligenes, 4-carboxyphenylacetic acid and 1-(4-carboxybutyl) indole-5-boric acid are used as composite cross-linking agents, the composite cross-linking agents and sodium alginate form a hydrogel carrier, the pseudomonas alcaligenes are efficiently encapsulated, a PD-1 antibody is loaded, the tumor weight of the pancreatic cancer is remarkably reduced, tumor growth is effectively inhibited, and the pancreatic cancer can be effectively inhibited. The triple barriers of pancreatic cancer immunotherapy are successfully broken through; the hydrogel carrier is high in bacterial encapsulation efficiency, can effectively construct a growth microenvironment suitable for pseudomonas alcaligenes and promote bacterial growth, and is excellent in biological safety; and a low-toxicity and high-efficiency brand-new technical scheme is provided for immunotherapy of pancreatic cancer.

The invention discloses a complex microbial inoculant for onions and a preparation method of the complex microbial inoculant. The complex microbial inoculant comprises acinetobacter JQ-MY-41 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.32731, pantoea JQ-MY-42 with the preservation number of CGMCC NO.32732 and alcaligenes YC-342 with the preservation number of CGMCC NO.32733, and the optimal mass ratio of the acinetobacter JQ-MY-41 to the pantoea JQ-MY-42 to the alcaligenes YC-342 to the alcaligenes YC-342 is 30: 25: 30. The preparation method comprises the steps of strain activation, strain culture, thallus collection, strain compounding, carrier addition, drying and the like. The three strains achieve synergistic growth promotion through different mechanisms such as phosphorus solubilization, nitrogen fixation and IAA production, and compared with a control group, the onion plant height is increased by 37.5%, the stem diameter is increased by 39.0%, the total biomass is increased by 103.7%, and the total chlorophyll amount is increased by 69.5%; the problems that an existing microbial agent is single in function and limited in growth promoting effect are solved, and technical support is provided for green and efficient cultivation of onions.

The invention belongs to the technical field of microorganisms, and discloses alcaligenes faecalis and application thereof in prevention and treatment of root rot of schisandra chinensis. The invention provides a strain of alcaligenes faecalis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32518. The alcaligenes faecalis provided by the invention has a relatively good prevention and treatment effect on schisandra chinensis root rot.

The application discloses a 3D bioprinting bacteria-algae symbiotic engineering living material and a preparation method and application thereof. The method takes chlorella as a biological oxygen supply source, takes alkali-producing bacteria as phenol-degrading bacteria, takes sodium alginate and carboxymethyl cellulose as carriers, and respectively prepares bacterial bio-ink and algal bio-ink added with silicon dioxide, so as to build bacteria-algae symbiotic engineering living materials with stripe and layer configurations through a 3D bioprinting mechanism. The bacteria-algae symbiotic engineering living material realizes three-dimensional accurate arrangement and functional partition of bacteria and algae, and exhibits excellent degradation performance and photosynthetic performance under different phenol concentrations, and has application prospects in the biological degradation treatment of organic pollutants.

The invention discloses a coupled nitrogen catabolism compound microbial agent and application thereof, and belongs to the technical field of biology. According to the method, an efficient ammonification strain (lysinibacillus L11) and a direct ammoxidation strain (alcaligenes M8) are screened, an ammonification-Dirammox coupling system is constructed, the strain co-culture time sequence (L11 is inoculated 48 hours earlier) is optimized, and organic nitrogen is directly converted into N2. According to the method, the total nitrogen removal rate in the synchronous ammoniation and nitrification culture medium reaches 96.84%, and the ammonia nitrogen removal rate reaches 99.37%. In practical application, the ammonia nitrogen in effluent of a sewage treatment system is stabilized at 9.44 mg / L and COD (Chemical Oxygen Demand) is reduced to 455.98 mg / L by adding the microbial agent, which is obviously superior to that of the traditional process. The method provided by the invention solves the problems of low treatment efficiency of high-ammonia-nitrogen wastewater and serious secondary pollution, and is suitable for co-treatment of aging leachate and perishable landfill leachate.

The invention relates to the technical field of environmental microorganisms, in particular to a mixed bacterial agent for denitrification of cyanogen-containing wastewater. The microbial inoculum is prepared from five strains, namely alcaligenes alcaligenes YH-2C1, sphingosynium CY-10, calcium acetate-acinetobacter TNJ-1, acinetobacter and rhodococcus ruber C1 according to a proportion, the tolerance concentration of the microbial inoculum to CN <-> concentration reaches 40mg / L, and the tolerance concentration to ammonia nitrogen reaches 2000mg / L. The microbial agent for denitrification of the cyanide-containing wastewater, provided by the invention, is mainly applied to the fields of denitrification of high-ammonia-nitrogen wastewater, denitrification of the cyanide-containing wastewater and the like, has the advantages of high removal efficiency, strong endurance capacity, convenience in use and culture and the like, and can quickly remove ammonia nitrogen and total nitrogen in water under the condition of containing high-concentration cyanide.

The invention relates to the technical field of microorganisms, and particularly discloses alcaligenes alcaligenes ALA-1 and application of the alcaligenes alcaligenes ALA-1 in nitrous oxide emission reduction. The invention provides an alcaligenes faecalis ALA-1 strain, and the preservation number of the alcaligenes faecalis ALA-1 strain is CGMCC (China General Microbiological Culture Collection Center) No. 35322. The method can efficiently reduce N2O into N2, is applied to the aerobic composting treatment process, can significantly reduce the N2O gas emission in the composting process, and promotes the green low-carbon development of the organic solid waste composting industry.

This invention discloses an engineered astaxanthin-producing probiotic yeast strain, its construction method, and its applications, belonging to the field of synthetic biology. The engineered strain uses β-carotene-producing *Saccharomyces cerevisiae* as the substrate cell. Its genome integrates a fusion gene selected through adaptability screening. This gene is composed of *BDC263crtW* from *Saccharomyces cerevisiae*, a flexible linker peptide, and *AspcrtZ* from *Alcaligenes aeruginosa*, linked sequentially. Simultaneously, based on systematic expression screening of multiple key genes, the endogenous acetyl-CoA synthesis key gene ACS1 was optimized and overexpressed, while the acetic acid metabolism negative regulatory gene YPL062W was knocked out. The construction method includes plasmid construction of the aforementioned fusion expression module and ACS1 expression module, and targeted integration into the genome. This invention significantly improves astaxanthin synthesis levels and enhances the engineered strain's tolerance to the simulated gastrointestinal environment and its antioxidant capacity through enzyme source optimization and substrate channel effects. This engineered strain can be directly used as a functional live bacteria preparation for food and feed additives, with broad prospects for industrial application.

The application belongs to the technical field of sewage treatment, and provides a direct ammonia oxidation denitrification device, which comprises biological filler, an aerobic reaction tank, a stirrer, aeration stones, a first water outlet, a second water inlet, a backflow water outlet, a sludge backflow pump, a backflow water inlet, a membrane tank and an MBR membrane assembly; the aerobic reaction tank is internally provided with biological filler and a stirrer; the bottom of the aerobic reaction tank is provided with aeration stones; the aeration stones are connected with an air pump; the top of the membrane tank is provided with a water inlet; the bottom of the membrane tank is provided with a backflow water outlet; the middle of the membrane tank is provided with an MBR membrane assembly; the biological filler is provided with a biological membrane of Alcaligenes sp. HO-1; and the filter diameter of the MBR membrane assembly is not greater than 0.20 microns. The sewage / wastewater biological denitrification device and process based on direct ammonia oxidation provided by the application have high denitrification efficiency and simple denitrification process.

This invention discloses a chemoautotrophic alkaloid-producing strain of *Alcaligenes rocheborella*, its construction method, and its application. The strain is based on the chemoautotrophic microorganism *Alcaligenes rocheborella* H16 (… Cupriavidus necator Using H16 as the starting strain, the poly-3-hydroxybutyrate (PHB) synthesis pathway of the strain was first knocked out, and then a strain from Rhodotorula glutinis (H16) was introduced. Rhodotorula glutinis ) tyrosine ammonia lyase gene tal A basic engineered strain capable of producing p-coumaric acid was constructed. Subsequently, the throughput of the p-coumaric acid synthesis pathway in the engineered strain was enhanced by overexpressing the endogenous 3-deoxy-7-phosphate heptanulate synthase gene aroG1 and the branching acid synthase gene aroC, and by increasing the tal copy number. Further enhancement was achieved by introducing strains from *Escherichia coli* (…). Escherichia coli By improving the supply of cofactors through the NAD(P) transhydrogenase gene pntAB, a strain of Alcaligenes roximatelye that can synthesize p-coumaric acid using CO2 was finally obtained.

The invention belongs to the technical field of wastewater treatment, and particularly relates to a bacterial agent for treating benzalkonium chloride-containing wastewater and an application thereof. The bacterial agent is obtained by regulating and controlling experimental environmental parameters and domesticating the bacterial agent, wherein the mass concentration percentage of each bacterial species is 32.37% of Pseudomonas, 21.94% of Rhizobium, 14.69% of Achromobacter, 4.65% of Stenotrophomonas, 3.79% of T. mobilis, 2.41% of Flavobacterium, 2.26% of Alcaligenes, 1.88% of Acetogenic Proteinophilus, 1.59% of Bacillus and 0.53% of Aeromonas. The bacterial agent can improve the degradation rate of COD in the benzalkonium chloride-containing wastewater.

The present application belongs to the technical field of water treatment, and particularly relates to a supported Alcaligenes faecalis composite material, a preparation method thereof and application thereof. The present application forms a composite material by loading Alcaligenes faecalis on phosphate functionalized biochar, utilizes the phosphorus dissolving and accumulating functions of Alcaligenes faecalis to convert the soluble phosphate groups on the surface and inside of the biochar into insoluble metaphosphate minerals and extracellular polymers, and achieves the effect of phosphorus fixation through the adsorption of metal cations in the biological membrane to phosphate; Alcaligenes faecalis can perform microbial phosphorus fixation, thereby improving the adsorption stability of the supported Alcaligenes faecalis composite material to uranium; through the interface synergistic effect of the biochar and Alcaligenes faecalis, the composite material has more excellent U(VI) adsorption performance (wide pH adaptation range, fast adsorption speed, high removal rate, and strong anti-interference ability) and reusability.

The invention relates to a preparation method of freeze-thaw-resistant immobilized particles and application of the freeze-thaw-resistant immobilized particles to degradation of enrofloxacin under freeze-thaw conditions. The immobilized particles mainly comprise sodium alginate, sodium pyrophosphate, sodium tripolyphosphate, sodium hexametaphosphate, cane sugar, zeolite and charcoal. When the enrofloxacin is degraded, an embedded bacterial strain is alcaligenes ammonoxydans, is named as Y-ENR, is preserved in the China General Microbiological Culture Collection Center, the address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No: 25659, and the sequence of the bacterial strain is as shown in SEQ ID No.1. The particles are high in mechanical strength, remarkable in freeze-thaw resistance and easy to recycle, and the effect of degrading enrofloxacin by the Y-ENR strain under the freeze-thaw condition can be remarkably improved. According to the invention, a treatment environment is expanded to a freeze-thaw condition, and support is provided for construction of an antibiotic biodegradation technology in winter cold regions in northern China.

The invention relates to alcaligenes faecalis AF12 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The alcaligenes faecalis AF12 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.37190, the preservation date is December 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides application of the alcaligenes faecalis AF12 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The alcaligenes faecalis AF12 provided by the invention can be applied to any plant species or plant habitat, realizes multiple effects of plant growth promotion, prevention and control of plant fungal diseases and bacterial diseases and prevention and control of plant root-knot nematode, and has extremely high application value and popularization prospect in the field of plant cultivation.

The invention relates to the technical field of flocculant development, in particular to a bio-based flocculant and a preparation method thereof.The bio-based flocculant comprises 15%-25% of cationic polyacrylamide, 23%-57% of polymeric silicate aluminum ferric sulfate, 6%-10% of heavy metal composite adsorption aid powder, 13%-23% of a magnetic adsorption aid, 7%-13% of composite bacterial powder, 2%-5% of a microbial deodorant, 4%-9% of a microbial oxygen aid and 2%-16% of an excipient. The heavy metal composite adsorption assistant powder comprises stenotrophomonas maltophilia, providencia alcaligenes and a mixture of metallophilic bacteria and lysinibacillus, the composite bacterial powder is a mixture of agaricus blazei hyphae, lentinus edodes mushroom residues, flammulina velutipes mushroom residues and oyster mushroom residues, and the microbial oxygen assistant is a mixture of cyanobacteria chlorophycocyanobacteria and zoogloea. On one hand, the flocculation speed and the decomposition speed of heavy metal impurities in sewage can be effectively increased, on the other hand, magnetic adsorption and rapid flocculation of the heavy metal impurities in the sewage can be achieved, and meanwhile, the flocculant can also achieve increase of the oxygen content of the sewage, so that the oxygen exposure purification efficiency during sewage flocculation is effectively improved.

This invention relates to the field of medical data processing technology, specifically to a diagnostic method for postmenopausal osteoporosis (PMOP) based on oral salivary bacteria. The method includes: collecting saliva samples from the subject in the morning on an empty stomach and labeling the subject's menopausal years; obtaining the relative abundance of Lactobacillus and Streptococcus, the concentrations of propionic acid and acetic acid, and the pH value; determining the corrected bone mineral density (BMD) correlation value using a diagnostic model; and outputting suggestions based on the BMD correlation value. This invention utilizes the principle that the decline in estrogen levels in postmenopausal women leads to an imbalance in the salivary flora, with an increase in acid-producing bacteria leading to increased acetic acid secretion and a decrease in alkali-producing bacteria leading to decreased propionic acid secretion. These two factors synergistically exacerbate osteogenic inhibition and increased bone resorption. Furthermore, since most saliva is partially absorbed after swallowing, the long-term accumulation of propionic acid and acetic acid can interfere with bone metabolism through the circulatory system, thus providing a preliminary diagnosis of PMOP. By collecting only saliva samples, without blood collection or radiation, the procedure is simple, painless, and provides physicians with preliminary diagnostic suggestions.

The invention discloses a complex microbial inoculant with a cellulose-protein two-component degradation function as well as a preparation method and application of the complex microbial inoculant. According to the present invention, the active components of the composite bacterial agent comprise Acinetobacter baumannii (Acinetobacter baumannii) and Providencia alcaligenes (Providencia alcaligenes), and the active components of the composite bacterial agent comprise a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain and a bacillus amyloliquefaciens strain, the number of the acinetobacter baumannii in the China Industrial Microbiological Culture Collection Center is CICC 22933; the providencia alcaligenes is numbered as CICC (China Industrial Microbiological Culture Collection Center) 25067 in the China Industrial Microbiological Culture Collection Center). The strains in the complex microbial inoculant do not have antagonism, and the complex microbial inoculant shows a remarkable synergistic effect when degrading cellulose and protein, so that the waste conversion efficiency and the yield of hermetia illucens can be synchronously improved, the environmental pollution is reduced, and a new technical support is provided for high-value utilization of agricultural wastes.