170 results about "Alcaligenes" patented technology

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium/micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

The invention provides a composite microbial biogen capable of degrading organic waste efficiently and a preparation method and applications thereof. The composite microbial biogen contains Bacillus circulans, Bacillus subtilis, Alcaligenes faecalis and Penicillium diversum. The composite microbial biogen can be used with activated sludge for the existing sewage treatment equipment; under the synergetic effect, the yield of excess sludge can be greatly reduced, the aims of emission reduction and energy saving can be realized and the sludge problem which is difficult to overcome for a long time in sludge treatment is effectively solved; and the biogen has importanct economic importance and environmental importance and is suitable for each sewage treatment plant. The composite microbial biogen can also be used to prepare organic wastewater purifying agents, activated sludge enhancers of urban domestic wastewater and deodorants used in tipping sites, public places, agricultural product market, kitchens, bathrooms and the like.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention provides a microbial soil remediating agent and a preparation method thereof, and relates to the field of soil remediation. The method comprises the following steps: uniformly mixing pseudomonas, trichoderma, penicillium, alcaligenes, bacillus licheniformis and bacillus subtilis to obtain a microbial agent; and uniformly mixing 20-40 parts by weigh of biomass, 60-75 parts by weight of nutritional agent, 3-5 parts by weight of microbial agent, 20-25 parts by weight of montmorillonite powder, 13-17 parts by weight of attapulgite, 18-32 parts by weight of kieselguhr, 13-20 parts byweight of sulfate filling agent, and 5-10 parts by weight of adsorbing agent to obtain the uniform microbial soil remediating agent. The microbial soil remediating agent is capable of remediating soilsubjected to heavy metal pollution, pesticide residue pollution, etc.; and the microbial soil remediating agent has the advantages of being good in treatment effect, and low in treatment cost.

The invention provides an Alcaligenes faecalis strain M50-B1, which has a preservation number of CGMCC 5219. The invention also provides application of the bacterium in a biological desulfurization deodorant. The bacterium is prepared into a liquid bacterial agent and a solid bacterial agent. During oxidation of hydrogen sulfide, no acid is produced. At the same time, a weak acidic bacterial liquid can absorb the ammonia in the air in the spraying process, thus achieving a good deodorization effect. The activated carbon in solid bacterial agents makes up the delay defect of biological deodorization, and the deodorizing cost is reduced. The Alcaligenes faecalis provided by the invention and the biological desulfurization deodorant prepared from the same have a good deodorization effect. The desulfurization deodorant has the advantages of simple preparation method, low cost, and no secondary pollution, thus being suitable for popularization and application in deodorization of household garbage and livestock and poultry production.

The invention provides a preparation method and an application of an attapulgite/nano-iron composite material-microorganism coupled body. The preparation method comprises the following steps: purifying attapulgite with distilled water and sodium hexametaphosphate, modifying by hydrochloric acid, conducting a centrifugal process, washing, drying, putting into a FeSO4 solution, soaking for 24-72 hours, conducting the centrifugal process, adding ethanol into a solid after the centrifugal process under the protection of argon gas, uniformly mixing, dropwise adding KBH4 for full reaction of Fe<2+>, conducting the centrifugal process, washing and drying deposits after the centrifugal process, and producing a coupling reaction with alcaligenes eutrophus to obtain a solid matter as the attapulgite/nano-iron composite material-microorganism coupled body. According to the coupled body, the shortcomings of poor nano-iron stability, poor dispersity, high possibility of conglomeration and the like are overcome, the defect of secondary pollution on a water body due to ammonia nitrogen generated during reaction of an attapulgite/nano-iron composite material with nitrate is avoided, and microorganisms basically cannot be poisoned.

The invention discloses a method for treating phenol-containing waste water by sawdust immobilized alcaligenes faecalis. The method has the advantages that (1) the immobilized alcaligenes faecalis is easy to culture and has the advantages of simple immobilization process, excellent catalytic degradation effect, resistance to high-concentration phenol-containing waste water with the concentration of phenol up to 3000mg.milligram<-1>, strong phenol decomposition capability and repeated use; (2) the carrier of the immobilized alcaligenes faecalis is sawdust, and modified utilization of the sawdust is realized by a chemical method, so the method improves the treatment efficiency for the phenol-containing waste water by means of microbial degradation, and has a broad development prospect; (3) the immobilized alcaligenes faecalis prepared through taking sawdust as the carrier has excellent elasticity, strong resistance to temperature and pH value, stable degradation property and long half life period; and (4) when used for treating the phenol-containing waste water, the sawdust immobilized alcaligenes faecalis has the advantages of simple process, low running cost, moderate conditions, environmental protection, uneasy loss of thalli, high concentration of microorganism inside the carrier, quick degradation speed, high degradation efficiency and no secondary pollution, and can realize continuous industrial degradation of the phenol-containing waste water at the same time.

The invention discloses microbial fertilizer and a preparation method thereof. The microbial fertilizer is fermentation liquor with hydrogen-oxidizing bacterium content ranging from 1X108/mL to 1X1010/mL or mixture of hydrogen-oxidizing bacterium liquid and sterile grass carbon, the content of hydrogen-oxidizing bacteria in the mixture ranges from 2X108/g to 2X1010/g, moisture of the mixture ranges from 20wt% to 40wt%, and the hydrogen-oxidizing bacteria include one type or a plurality of types of a flavobacterium, a pseudomonas, alcaligenes and burkholderia. The invention further provides a preparation method of the microbial fertilizer. The microbial fertilizer realizes a fine application effect for plants, and is free of environmental pollution, low in consumption and easy in popularization, the preparation process of the microbial fertilizer is simple, the production cycle is short, and cost is low.

The present invention relates to a recombinant Escherichia coli having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate using the same, more precisely, a recombinant Escherichia coli ‘MG1655/pTZ18U-PHB’ or ‘JIL938/pTZ18U-PHB’ having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate comprising the steps of preparing transformed Escherichia coli having phbCAB originated from Alcaligenes eutrophus, inoculating and culturing the cells (growth phase), inducing the production of polyhydroxyalkanoate in the recomibnant Escherichia coli (stationary phase and producing phase), and inducing the extracellular secretion of the polyhydroxyalkanoate from the recombinant Escherichia coli. The method of the present invention facilitates not only overproduction of polyhydroxyalkanoate such as polyhydroxybutyrate by a simple batch culture but also industrial use of biodegradable polyhydroxyalkanoate replacing conventional non-biodegradable plastics by its simple and easy fermentation, separation and purification process.

The invention discloses an efficient sewage treatment microbial agent. The efficient sewage treatment microbial agent is prepared from, by weight, 2-4 parts of nitrosobacteria, 10-20 parts of bacillus subtilis, 8-15 parts of alcaligenes faecalis, 5-10 parts of bacillus megatherium, 2-8 parts of pseudomonas aeruginosa, 2-10 parts of candida lipolytica, 2-8 parts of deoxidized paracoccus, 2-6 parts of pseudomonas stutzeri, 2-10 parts of saccharomyces cerevisiae, 1-4 parts of aspergillus niger, 0.1-0.6 part of yeast extract, 2-6 parts of peptone, 0.1-0.5 part of glucose, 8-15 parts of kaolin, 2-5 parts of aluminum polychlorid, 0.1-0.5 part of FMEE and a proper amount of water. The efficient sewage treatment microbial agent is suitable for treating industrial waste water, breeding waste water and the like, all strains are in cooperation, symbiotic coordination is achieved, high activity is achieved, and reproduction is fast. The efficient sewage treatment microbial agent is put in the waste water to be treated, the degradation effect can be effectively accelerated, the treatment water quality can be greatly improved, operating cost can be reduced, the treated water reaches the standard to be drained, and damage to environment is reduced.

The invention belongs to the technical field of environmental protection, and discloses a sewage treatment method for garden planning. The sewage treatment method comprises the following steps: performing treatment through a preliminary sedimentation tank; performing filtration; performing secondary precipitation; performing treatment by a biological reaction tank. Mixed bacterium liquid being prepared from actinomycetes, bacillus alcaligenes, bacillus pumilus, candida albicans, bacillus amyloliquefaciens, papyrosolvens and paracoccus denitrificans is added into the biological reaction tank, sewage treatment is performed, 10 grams of a microbiological preparation is added into each cubic meter of liquid each time, adding is performed once every day and continually performed for one week, and finally standing is performed for 3 days, so that liquid is drained for garden irrigation. The method disclosed by the invention is suitable for sewage treatment of gardens, and treated water can be used for greening of communities and gardens, sprinkling to roads and the like, so that circular economic utilization of water is realized.

The invention discloses a method for improving a paper strengthening effect of a bacterial cellulose-based paper strengthening agent. Bacterial cellulose disclosed by the invention is cellulose secreted by bacterial microbes and synthesized under different culture conditions, and has an English name of Bacterial Cellulose (BC for short). The microbes secreting the cellulose comprise acetobacter, agrobacterium, pseudomonas, achrombacter, alcaligenes, aerobacter, azotobacter, rhizabium, sarcina and the like. The paper strengthening agent refers to an assistant which is added in the paper making process of wood fibers, non-wood vegetable fibers or secondary fiber pulp and can be used for strengthening physical properties of paper. According to the method for improving the effect of the bacterial cellulose-based paper strengthening agent, disclosed by the invention, quaternary ammonium salt cationic modification is performed on the bacterial cellulose, so that the effect of strengthening the physical properties of the paper by the bacterial cellulose can be greatly improved or the consumption of the bacterial cellulose is reduced while an equivalent strengthening effect is achieved.

The invention belongs to the field of microbial application, and particularly relates to alcaligenes faecalis and application thereof. According to the specific technical scheme, the alcaligenes faecalis is preserved in China General Microbiological Culture Collection Center on June 11, 2018, the strain name is alcaligenes faecalis DFN2, and the preservation number is CGMCC No.15926. The alcaligenes faecalis has an excellent degradation effect on common malodorous substances such as ammonia gas and hydrogen sulfide, and the maximum degradation rate of the ammonia gas and the hydrogen sulfide can reach 100%; and the degradation rate of an ammonia nitrogen substance in high-ammonia-nitrogen wastewater can reach up to 99.5%. The alcaligenes faecalis can also carry out biological desulfurization treatment on thiophene sulfide, and the desulfurization effect is obviously superior to a universal hydrodesulfurization effect.

The invention discloses a method for treating domestic wet garbage; the method comprises the following steps of: step 1: collecting the domestic wet garbage and spraying the compound microorganism tothe domestic wet garbage, wherein the compound microorganism is the compound comprising photosynthetic bacteria and flora, yeast and flora, actinomycetes and flora, nitrogen-fixing bacteria and flora,lactic acid bacteria and flora, bifidobacteria and flora, bacillus and flora, DL bacteria and flora, and Trichoderma and flora; step 2, pulverizing and extruding the domestic wet garbage to perform solid-liquid separation; and obtaining a solid substance and a liquid substance containing the compound microorganism; step 3, packaging the solid substance, sprinkling silicate bacteria and alcaligenes faecalis in the bag; conducting the compost treatment after sealing the bag; and obtaining a first organic fertilizer; step 4, conducting oil-water separation to the liquid substance, and obtaininggrease and waste water; and step 5, treating the wastewater by a treatment device. The method provided by the invention has high comprehensive utilization rate without secondary products, and can realize efficient treatment and utilization of the domestic wet garbage.

The present invention provides a method for producing selenium-enriched combined nitrogen-fixing bacteria agent and its freeze-dry powder, liquid fertilizer and solid fertilizer. The culture medium of selenium-enriched combined nitrogen-fixing bacteria is composed of Na2SeO3, peptone, yeast, rare earth aqueous solution, NaCl, water etc. Three strains are respectively inoculated in flasks. Alcaligenes faecalis is represented by A15, nitrogen-fixing giant bacillus is represented by N13, Enterobacter Cloacae is represented by E26. Liquid bacterial fertilizer is obtained via rocking bottle enlarged cultivation, seed tank culture, liquid ferment in ferment tank, preservation and adjusting pH. If it is processed with vacuum freeze-dry, the freeze-dry powder is obtained. The solid fertilizer culture medium comprises grass charcoal, chicken dung, gypsum, rare earth, KH2PO4,Na2SeO3 5H2O. Fermented bacteria strain in ferment tank is inoculated at solid fertilizer culture medium and the final product is obtained after compost. The present inventive process is practical and suitable for industrial production.

The invention discloses a preparation for restoring ammonia nitrogen-containing industrial sewage. The preparation is prepared from the following components: 100 parts of thiobacillus denitrificans, 80 parts of bacillus licheniformis, 60 parts of sphingomonas, 50 parts of phanerochaete chrysosporium, 40 parts of arthrobacter globiformis, 30 parts of alcaligenes faecalis, 10 parts of pseudomonas aeruginosa and 10 parts of bacillus cereus. The invention also discloses a preparation method of the preparation. The preparation disclosed by the invention is suitable for the treatment of high-ammonia nitrogen industrial sewage, is energy-saving and environment-friendly, and has better application prospects.

The present invention discloses one kind of Bacillus alcaligenes and the process of preparing single antimer mandelic acid therewith. The resting cell of the Bacillus alcaligenes, ECU0401 CGMCC No.2009, is used as the biological catalyst in selectively converting racemized mandelic acid and its derivative to prepare optically active mandelic acid and its derivative. The Bacillus alcaligenes strain of the present invention has high catalyzing effect, and the product (R)-(-)-mandelic acid has optical purity over 99.9 %, obviously higher than that obtained with catalyzing yellow Bacillus brevis AS1.818 and with catalyzing Saccharomyces cervisiae AS2.150.