170 results about "Alcaligenes" patented technology

The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium / micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

The invention relates to a method for producing coenzyme Q10, which comprises the following steps: firstly, high density fermentation is performed to true alcaligenes eutruphus till the dry weight of thallus is above 90 gram / liter; secondly, the thallus is separated from a fermentation liquid; thirdly, the coenzyme Q10 is extracted from the separated thallus. The invention also relates to an application of true alcaligenes eutruphus in the coenzyme Q10 produced through the high density fermentation.

The invention provides a composite microbial biogen capable of degrading organic waste efficiently and a preparation method and applications thereof. The composite microbial biogen contains Bacillus circulans, Bacillus subtilis, Alcaligenes faecalis and Penicillium diversum. The composite microbial biogen can be used with activated sludge for the existing sewage treatment equipment; under the synergetic effect, the yield of excess sludge can be greatly reduced, the aims of emission reduction and energy saving can be realized and the sludge problem which is difficult to overcome for a long time in sludge treatment is effectively solved; and the biogen has importanct economic importance and environmental importance and is suitable for each sewage treatment plant. The composite microbial biogen can also be used to prepare organic wastewater purifying agents, activated sludge enhancers of urban domestic wastewater and deodorants used in tipping sites, public places, agricultural product market, kitchens, bathrooms and the like.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention provides a microbial soil remediating agent and a preparation method thereof, and relates to the field of soil remediation. The method comprises the following steps: uniformly mixing pseudomonas, trichoderma, penicillium, alcaligenes, bacillus licheniformis and bacillus subtilis to obtain a microbial agent; and uniformly mixing 20-40 parts by weigh of biomass, 60-75 parts by weight of nutritional agent, 3-5 parts by weight of microbial agent, 20-25 parts by weight of montmorillonite powder, 13-17 parts by weight of attapulgite, 18-32 parts by weight of kieselguhr, 13-20 parts byweight of sulfate filling agent, and 5-10 parts by weight of adsorbing agent to obtain the uniform microbial soil remediating agent. The microbial soil remediating agent is capable of remediating soilsubjected to heavy metal pollution, pesticide residue pollution, etc.; and the microbial soil remediating agent has the advantages of being good in treatment effect, and low in treatment cost.

The invention discloses alcaligenes faecalis (Alcaligenes faecalis) YS302. The alcaligenes faecalis YS302 is collected in China General Microbiological Culture Collection Center (called CGMCC for short) on December 26, 2016 and has a collection number of CGMCC NO. 13502. The invention further discloses application of the alcaligenes faecalis. The invention has the advantages that only the alcaligenes faecalis YS302 needs to be added, extra packing does not need to be added, ammonia nitrogen and hydrogen sulfide in sewage and stench can be simultaneously degraded, the sewage treatment cost is reduced, the cost and space and time needed for treatment can be further reduced, and a good method and technical support can be provided for environmental workers.

The invention provides an Alcaligenes faecalis strain M50-B1, which has a preservation number of CGMCC 5219. The invention also provides application of the bacterium in a biological desulfurization deodorant. The bacterium is prepared into a liquid bacterial agent and a solid bacterial agent. During oxidation of hydrogen sulfide, no acid is produced. At the same time, a weak acidic bacterial liquid can absorb the ammonia in the air in the spraying process, thus achieving a good deodorization effect. The activated carbon in solid bacterial agents makes up the delay defect of biological deodorization, and the deodorizing cost is reduced. The Alcaligenes faecalis provided by the invention and the biological desulfurization deodorant prepared from the same have a good deodorization effect. The desulfurization deodorant has the advantages of simple preparation method, low cost, and no secondary pollution, thus being suitable for popularization and application in deodorization of household garbage and livestock and poultry production.

The invention provides a preparation method and an application of an attapulgite / nano-iron composite material-microorganism coupled body. The preparation method comprises the following steps: purifying attapulgite with distilled water and sodium hexametaphosphate, modifying by hydrochloric acid, conducting a centrifugal process, washing, drying, putting into a FeSO4 solution, soaking for 24-72 hours, conducting the centrifugal process, adding ethanol into a solid after the centrifugal process under the protection of argon gas, uniformly mixing, dropwise adding KBH4 for full reaction of Fe<2+>, conducting the centrifugal process, washing and drying deposits after the centrifugal process, and producing a coupling reaction with alcaligenes eutrophus to obtain a solid matter as the attapulgite / nano-iron composite material-microorganism coupled body. According to the coupled body, the shortcomings of poor nano-iron stability, poor dispersity, high possibility of conglomeration and the like are overcome, the defect of secondary pollution on a water body due to ammonia nitrogen generated during reaction of an attapulgite / nano-iron composite material with nitrate is avoided, and microorganisms basically cannot be poisoned.

The invention discloses a microbial strain which can effectively degrade phenol and relates to alcaligenes faecalis JH1013 CCTCC NO: M 208006 and a method for degrading phenol wastewater by using a fixed alcaligenes faecalis that is produced by alcaligenes faecalis. The method has the following advantages that: 1. the alcaligenes faecalis is easy to culture, the fixing technique is simple, the catalytic degradation effect is good, the high-concentration phenol can be resistance, the phenol degradation capacity is strong, which can high efficiently degrade the phenol wastewater with the concentration of as high as 2000mg. L<-1> and be used repeatedly; 2. hydrotalcite, hydrotalcite-like compounds, nano calcium carbonate, Gamma-Al2O3 or the loading solid base are used as carriers for preparing the fixed alcaligenes faecalis, the elasticity is good, the temperature and pH value resistances are strong, the mechanical strength is high, the degradation performance is stable and the half-time is long; 3. the carriers of the hydrotalcite, the hydrotalcite-like compounds, the nano calcium carbonate and the Gamma-Al2O3 of the fixed cell can be used repeatedly by calcinations; 4. the fixed alcaligenes faecalis is used for degrading the phenol, the degradation process is simple, the conditions are moderate, the environment is friendly, the bacterial cells are difficult to loss, the concentration of the microorganisms in the carriers is high, the degradation speed is fast, the degradation efficiency is high and the secondary pollution is not easy to be caused.

The invention discloses a method for treating phenol-containing waste water by sawdust immobilized alcaligenes faecalis. The method has the advantages that (1) the immobilized alcaligenes faecalis is easy to culture and has the advantages of simple immobilization process, excellent catalytic degradation effect, resistance to high-concentration phenol-containing waste water with the concentration of phenol up to 3000mg.milligram<-1>, strong phenol decomposition capability and repeated use; (2) the carrier of the immobilized alcaligenes faecalis is sawdust, and modified utilization of the sawdust is realized by a chemical method, so the method improves the treatment efficiency for the phenol-containing waste water by means of microbial degradation, and has a broad development prospect; (3) the immobilized alcaligenes faecalis prepared through taking sawdust as the carrier has excellent elasticity, strong resistance to temperature and pH value, stable degradation property and long half life period; and (4) when used for treating the phenol-containing waste water, the sawdust immobilized alcaligenes faecalis has the advantages of simple process, low running cost, moderate conditions, environmental protection, uneasy loss of thalli, high concentration of microorganism inside the carrier, quick degradation speed, high degradation efficiency and no secondary pollution, and can realize continuous industrial degradation of the phenol-containing waste water at the same time.

The invention discloses microbial fertilizer and a preparation method thereof. The microbial fertilizer is fermentation liquor with hydrogen-oxidizing bacterium content ranging from 1X108 / mL to 1X1010 / mL or mixture of hydrogen-oxidizing bacterium liquid and sterile grass carbon, the content of hydrogen-oxidizing bacteria in the mixture ranges from 2X108 / g to 2X1010 / g, moisture of the mixture ranges from 20wt% to 40wt%, and the hydrogen-oxidizing bacteria include one type or a plurality of types of a flavobacterium, a pseudomonas, alcaligenes and burkholderia. The invention further provides a preparation method of the microbial fertilizer. The microbial fertilizer realizes a fine application effect for plants, and is free of environmental pollution, low in consumption and easy in popularization, the preparation process of the microbial fertilizer is simple, the production cycle is short, and cost is low.

The invention relates to a culture method for Alcaligenes faecalis strain KZJ01 from oceans, characterized in that the Alcaligenes faecalis strain KZJ01 is obtained by the steps of enrichment culture, separation of strains, purification of strains, sieving of strains, authentication of strains and the like. The Alcaligenes faecalis strain KZJ01 obtained by the inventive culture method has the effect of inhibiting the growth of hypha of 9 plant pathogenic fungi and of inhibiting the germination of conidia of 2 plant pathogenic fungi therein.

The present invention relates to a recombinant Escherichia coli having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate using the same, more precisely, a recombinant Escherichia coli ‘MG1655 / pTZ18U-PHB’ or ‘JIL938 / pTZ18U-PHB’ having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate comprising the steps of preparing transformed Escherichia coli having phbCAB originated from Alcaligenes eutrophus, inoculating and culturing the cells (growth phase), inducing the production of polyhydroxyalkanoate in the recomibnant Escherichia coli (stationary phase and producing phase), and inducing the extracellular secretion of the polyhydroxyalkanoate from the recombinant Escherichia coli. The method of the present invention facilitates not only overproduction of polyhydroxyalkanoate such as polyhydroxybutyrate by a simple batch culture but also industrial use of biodegradable polyhydroxyalkanoate replacing conventional non-biodegradable plastics by its simple and easy fermentation, separation and purification process.

The invention relates to a dry biological demulsifier powder and an alcaligenes sp. that are used for demulsification in oil exploiting industries. The alcaligenes sp. in oil-polluted oilfield soil is selected, a whole liquid culture medium of the alcaligenes sp. is centrifugalized at the speed of 10000rpm, and a wet mycelium is obtained, baked at the temperature of 105 DEG C for 24 hours and grinded, thus obtaining the dry biological demulsifier powder. From the field tests in oilfield spots, the dry biological demulsifier powder can achieve the dehydration rate of 66.7 to 96.5 percent in 120 to 150 minutes when the addition amount is 100 to 200 milligrams per liter, thus overmatching chemical demulsifiers that are used on the spot. The dry biological demulsifier powder is produced by parching the strain culture medium with demulsification effect, thus solving the inconvenience of the biological demulsifiers with a whole liquid culture medium in the aspects of sale, transportation, storage and the like, and lowering the possibilities that the metamorphic whole liquid culture medium causes the reduction of demulsification effect and other components in the whole liquid culture medium affect oil products and subsequent water processing effect.

The invention discloses an efficient sewage treatment microbial agent. The efficient sewage treatment microbial agent is prepared from, by weight, 2-4 parts of nitrosobacteria, 10-20 parts of bacillus subtilis, 8-15 parts of alcaligenes faecalis, 5-10 parts of bacillus megatherium, 2-8 parts of pseudomonas aeruginosa, 2-10 parts of candida lipolytica, 2-8 parts of deoxidized paracoccus, 2-6 parts of pseudomonas stutzeri, 2-10 parts of saccharomyces cerevisiae, 1-4 parts of aspergillus niger, 0.1-0.6 part of yeast extract, 2-6 parts of peptone, 0.1-0.5 part of glucose, 8-15 parts of kaolin, 2-5 parts of aluminum polychlorid, 0.1-0.5 part of FMEE and a proper amount of water. The efficient sewage treatment microbial agent is suitable for treating industrial waste water, breeding waste water and the like, all strains are in cooperation, symbiotic coordination is achieved, high activity is achieved, and reproduction is fast. The efficient sewage treatment microbial agent is put in the waste water to be treated, the degradation effect can be effectively accelerated, the treatment water quality can be greatly improved, operating cost can be reduced, the treated water reaches the standard to be drained, and damage to environment is reduced.

The invention belongs to the technical field of environmental protection, and discloses a sewage treatment method for garden planning. The sewage treatment method comprises the following steps: performing treatment through a preliminary sedimentation tank; performing filtration; performing secondary precipitation; performing treatment by a biological reaction tank. Mixed bacterium liquid being prepared from actinomycetes, bacillus alcaligenes, bacillus pumilus, candida albicans, bacillus amyloliquefaciens, papyrosolvens and paracoccus denitrificans is added into the biological reaction tank, sewage treatment is performed, 10 grams of a microbiological preparation is added into each cubic meter of liquid each time, adding is performed once every day and continually performed for one week, and finally standing is performed for 3 days, so that liquid is drained for garden irrigation. The method disclosed by the invention is suitable for sewage treatment of gardens, and treated water can be used for greening of communities and gardens, sprinkling to roads and the like, so that circular economic utilization of water is realized.

The present invention relates to a recombinant Escherichia coli having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate using the same, more precisely, a recombinant Escherichia coli ‘MG1655 / pTZ18U-PHB’ or ‘JIL938 / pTZ18U-PHB’ having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate comprising the steps of preparing transformed Escherichia coli having phbCAB originated from Alcaligenes eutrophus, inoculating and culturing the cells (growth phase), inducing the production of polyhydroxyalkanoate in the recomibnant Escherichia coli (stationary phase and producing phase), and inducing the extracellular secretion of the polyhydroxyalkanoate from the recombinant Escherichia coli. The method of the present invention facilitates not only overproduction of polyhydroxyalkanoate such as polyhydroxybutyrate by a simple batch culture but also industrial use of biodegradable polyhydroxyalkanoate replacing conventional non-biodegradable plastics by its simple and easy fermentation, separation and purification process.

The invention relates to a corn straw compost for improving saline-alkali soil. The corn straw compost comprises corn straw powder, a flora suspension, ammonium nitrate, potassium dihydrogen phosphate, potassium chloride, zinc sulfate and water, wherein above florae comprise Bacillus thuringiensis, Alcaligenes faecalis, bacillus cereus and Cellulosimicrobium sp. 0707K4-3. The wheat field application method of the corn straw compost for improving saline-alkali soil is characterized in that: bacillus coagulans is cultured by a beef extract peptone medium and is prepared into the flora suspension by sterile normal saline, and wheat seeds are immersed in hydrogen peroxide to disinfect, is rinsed by sterile water, and then is immersed in the prepared flora suspension; and the compost is scattered to and uniformly mixed with flattened water-permeated soil, and the immersed wheat seeds are sowed in the soil. The corn straw compost of the invention has the advantages of improvement of physical and chemical properties of the saline-alkali soil, and wheat output improvement by carrying out degradation and microbial compost fermentation on corn straw; and the method of the invention, which allows the comprehensive utilization of the corn straw to be promoted, can be widely applied to the agricultural production and the saline-alkali soil improvement.

The invention discloses a method for improving a paper strengthening effect of a bacterial cellulose-based paper strengthening agent. Bacterial cellulose disclosed by the invention is cellulose secreted by bacterial microbes and synthesized under different culture conditions, and has an English name of Bacterial Cellulose (BC for short). The microbes secreting the cellulose comprise acetobacter, agrobacterium, pseudomonas, achrombacter, alcaligenes, aerobacter, azotobacter, rhizabium, sarcina and the like. The paper strengthening agent refers to an assistant which is added in the paper making process of wood fibers, non-wood vegetable fibers or secondary fiber pulp and can be used for strengthening physical properties of paper. According to the method for improving the effect of the bacterial cellulose-based paper strengthening agent, disclosed by the invention, quaternary ammonium salt cationic modification is performed on the bacterial cellulose, so that the effect of strengthening the physical properties of the paper by the bacterial cellulose can be greatly improved or the consumption of the bacterial cellulose is reduced while an equivalent strengthening effect is achieved.

The invention relates to the microbial field, in particular to a microorganism agent for recovering the organic polluted water and preparation process and using method. The microorganism agent consists of facultative anaerobics including Pseudomonas, Alcaligenes, Bacillus and Enterbacter with the weight ratio of 1:1-3:1-4:2-7, and the residual is the culture liquid containing nitrobenzene; the degradation rate can reach between 97.7 percent and 99.6 percent when the invention is implemented in the polluted water containing nitrobenzene. The microorganism agent of the invention is made by that the nitryl aromatic hydrocarbon compound and the nutrition compound with relatively high concentration are acclimatized and sorted, and has strong growing and reproductive ability as well as adaptability; in addition, the invention can assure a higher biomass in the environment at low cost, and does not cause the secondary pollution, and has the effect of continuously strengthening the purification of the water, thereby having good social, economic and ecological benefits.

The invention provides an alcaligenes faecalis Y311, and a screening method and application thereof. The strain is separated from tilapia culture water environment sediment, and has the preservation number of GDMCC No. 60350. According to the alcaligenes faecalis Y311, the removal rate of ammonia nitrogen in culture wastewater reaches 60.3% within 7 days, the removal rate of nitrate nitrogen reaches 74.7%, and the removal rate of COD reaches 36.7%. Under the aerobic condition, environmental probiotics of ammonia nitrogen, nitrate nitrogen and COD are efficiently degraded, and the alcaligenes faecalis Y311 has a higher application value.

The invention belongs to the field of microbial application, and particularly relates to alcaligenes faecalis and application thereof. According to the specific technical scheme, the alcaligenes faecalis is preserved in China General Microbiological Culture Collection Center on June 11, 2018, the strain name is alcaligenes faecalis DFN2, and the preservation number is CGMCC No.15926. The alcaligenes faecalis has an excellent degradation effect on common malodorous substances such as ammonia gas and hydrogen sulfide, and the maximum degradation rate of the ammonia gas and the hydrogen sulfide can reach 100%; and the degradation rate of an ammonia nitrogen substance in high-ammonia-nitrogen wastewater can reach up to 99.5%. The alcaligenes faecalis can also carry out biological desulfurization treatment on thiophene sulfide, and the desulfurization effect is obviously superior to a universal hydrodesulfurization effect.

The invention discloses a method for treating domestic wet garbage; the method comprises the following steps of: step 1: collecting the domestic wet garbage and spraying the compound microorganism tothe domestic wet garbage, wherein the compound microorganism is the compound comprising photosynthetic bacteria and flora, yeast and flora, actinomycetes and flora, nitrogen-fixing bacteria and flora,lactic acid bacteria and flora, bifidobacteria and flora, bacillus and flora, DL bacteria and flora, and Trichoderma and flora; step 2, pulverizing and extruding the domestic wet garbage to perform solid-liquid separation; and obtaining a solid substance and a liquid substance containing the compound microorganism; step 3, packaging the solid substance, sprinkling silicate bacteria and alcaligenes faecalis in the bag; conducting the compost treatment after sealing the bag; and obtaining a first organic fertilizer; step 4, conducting oil-water separation to the liquid substance, and obtaininggrease and waste water; and step 5, treating the wastewater by a treatment device. The method provided by the invention has high comprehensive utilization rate without secondary products, and can realize efficient treatment and utilization of the domestic wet garbage.

The present invention provides a method for producing selenium-enriched combined nitrogen-fixing bacteria agent and its freeze-dry powder, liquid fertilizer and solid fertilizer. The culture medium of selenium-enriched combined nitrogen-fixing bacteria is composed of Na2SeO3, peptone, yeast, rare earth aqueous solution, NaCl, water etc. Three strains are respectively inoculated in flasks. Alcaligenes faecalis is represented by A15, nitrogen-fixing giant bacillus is represented by N13, Enterobacter Cloacae is represented by E26. Liquid bacterial fertilizer is obtained via rocking bottle enlarged cultivation, seed tank culture, liquid ferment in ferment tank, preservation and adjusting pH. If it is processed with vacuum freeze-dry, the freeze-dry powder is obtained. The solid fertilizer culture medium comprises grass charcoal, chicken dung, gypsum, rare earth, KH2PO4,Na2SeO3 5H2O. Fermented bacteria strain in ferment tank is inoculated at solid fertilizer culture medium and the final product is obtained after compost. The present inventive process is practical and suitable for industrial production.

The invention discloses a preparation for restoring ammonia nitrogen-containing industrial sewage. The preparation is prepared from the following components: 100 parts of thiobacillus denitrificans, 80 parts of bacillus licheniformis, 60 parts of sphingomonas, 50 parts of phanerochaete chrysosporium, 40 parts of arthrobacter globiformis, 30 parts of alcaligenes faecalis, 10 parts of pseudomonas aeruginosa and 10 parts of bacillus cereus. The invention also discloses a preparation method of the preparation. The preparation disclosed by the invention is suitable for the treatment of high-ammonia nitrogen industrial sewage, is energy-saving and environment-friendly, and has better application prospects.

The present invention discloses one kind of Bacillus alcaligenes and the process of preparing single antimer mandelic acid therewith. The resting cell of the Bacillus alcaligenes, ECU0401 CGMCC No.2009, is used as the biological catalyst in selectively converting racemized mandelic acid and its derivative to prepare optically active mandelic acid and its derivative. The Bacillus alcaligenes strain of the present invention has high catalyzing effect, and the product (R)-(-)-mandelic acid has optical purity over 99.9 %, obviously higher than that obtained with catalyzing yellow Bacillus brevis AS1.818 and with catalyzing Saccharomyces cervisiae AS2.150.