14 results about "Alcaligenes" patented technology

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

The present application belongs to the technical field of sewage treatment, and particularly relates to a method for simultaneously improving the denitrification efficiency of sewage and degrading new pollutants. Alcaligenes The present application adopts a new strain of Alcaligenes sp. FDN-09 which is resistant to perfluorinated compounds to carry out large-scale culture, and prepares a high-efficiency functional bacterial agent; under the condition that ammonia nitrogen and new pollutants coexist in sewage, the high-efficiency functional bacterial agent is added in a biological reactor, and the reactor is operated under anoxic and low-oxygen conditions, thereby simultaneously strengthening the removal of ammonia nitrogen and new pollutants in the sewage, and achieving the effect of improving the denitrification of the sewage treatment system while degrading new pollutants. The method of the present application has no secondary pollution to the environment, has high simultaneous degradation rates of total nitrogen and new pollutants, low treatment cost, and low carbon emission. Under the condition that ammonia nitrogen and new pollutants coexist in the sewage, the water quality of the effluent is far lower than the first-level A effluent standard in the modification single of the Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB 18918-2002).

The invention provides a carbon sink type tailing soil conditioner as well as a preparation method and a soil treatment method thereof. The invention relates to a carbon sink type tailing soil conditioner, which comprises porous biochar loaded with an alkali-producing microbial agent and a multifunctional composite microbial agent with heavy metal toxicity reduction and efficient carbon sequestration effects; the alcaligenes microbial agent comprises alcaligenes; the multifunctional complex microbial inoculant comprises heavy metal passivating bacteria, carbonic anhydrase secreting bacteria and mycorrhizal fungi; the mycorrhizal fungi comprise at least one of arbuscular mycorrhizal fungi and ectotrophic mycorrhizal fungi; the porous biochar is prepared from the following raw materials: sodium carbonate and organic waste powder. According to the method, the rapid weathering of the tailings, the reconstruction of the soil structure and the efficient storage of CO2 are realized, the soil period is shortened from more than 5 years to 8-12 months, the carbon sink strength is improved to 2.1-3.4 t CO2 / ha.a, and an innovative solution is provided for the goals of ecological restoration and carbon neutralization of mines.

This invention discloses a strain of *Alcaligenes* HHR-1-1 and its application in the degradation of carotenoids and the preparation of flavorings, relating to the field of microbial fermentation technology. The *Alcaligenes* strain of this invention (… Pseudomonas alcaligenes The HHR-1-1 strain has the accession number CCTCC NO: M 20252745, and the *Alcaligenes* HHR-1-1 strain was deposited at the China Center for Type Culture Collection on December 1, 2025. The *Alcaligenes* HHR-1-1 strain can degrade carotenoids with a degradation rate of 80%–97%, and its main degradation products are β-ionone and its oxides, and dihydroactinolone. Furthermore, the fermentation broth of *Alcaligenes* HHR-1-1 strain and carotenoids can be used in flavor preparation, effectively improving the quality of the flavors.

The invention belongs to the field of tumor immunity, discloses application of a pseudomonas alcaligenes composition in preparation of pancreatic cancer treatment drugs, and aims to solve the technical problems that pancreatic cancer immunotherapy is low in response rate and existing treatment schemes are high in toxicity. The pseudomonas alcaligenes used in the invention are sPseudomonas alcaligenes, 4-carboxyphenylacetic acid and 1-(4-carboxybutyl) indole-5-boric acid are used as composite cross-linking agents, the composite cross-linking agents and sodium alginate form a hydrogel carrier, the pseudomonas alcaligenes are efficiently encapsulated, a PD-1 antibody is loaded, the tumor weight of the pancreatic cancer is remarkably reduced, tumor growth is effectively inhibited, and the pancreatic cancer can be effectively inhibited. The triple barriers of pancreatic cancer immunotherapy are successfully broken through; the hydrogel carrier is high in bacterial encapsulation efficiency, can effectively construct a growth microenvironment suitable for pseudomonas alcaligenes and promote bacterial growth, and is excellent in biological safety; and a low-toxicity and high-efficiency brand-new technical scheme is provided for immunotherapy of pancreatic cancer.

The application discloses a 3D bioprinting bacteria-algae symbiotic engineering living material and a preparation method and application thereof. The method takes chlorella as a biological oxygen supply source, takes alkali-producing bacteria as phenol-degrading bacteria, takes sodium alginate and carboxymethyl cellulose as carriers, and respectively prepares bacterial bio-ink and algal bio-ink added with silicon dioxide, so as to build bacteria-algae symbiotic engineering living materials with stripe and layer configurations through a 3D bioprinting mechanism. The bacteria-algae symbiotic engineering living material realizes three-dimensional accurate arrangement and functional partition of bacteria and algae, and exhibits excellent degradation performance and photosynthetic performance under different phenol concentrations, and has application prospects in the biological degradation treatment of organic pollutants.

This invention discloses an engineered astaxanthin-producing probiotic yeast strain, its construction method, and its applications, belonging to the field of synthetic biology. The engineered strain uses β-carotene-producing *Saccharomyces cerevisiae* as the substrate cell. Its genome integrates a fusion gene selected through adaptability screening. This gene is composed of *BDC263crtW* from *Saccharomyces cerevisiae*, a flexible linker peptide, and *AspcrtZ* from *Alcaligenes aeruginosa*, linked sequentially. Simultaneously, based on systematic expression screening of multiple key genes, the endogenous acetyl-CoA synthesis key gene ACS1 was optimized and overexpressed, while the acetic acid metabolism negative regulatory gene YPL062W was knocked out. The construction method includes plasmid construction of the aforementioned fusion expression module and ACS1 expression module, and targeted integration into the genome. This invention significantly improves astaxanthin synthesis levels and enhances the engineered strain's tolerance to the simulated gastrointestinal environment and its antioxidant capacity through enzyme source optimization and substrate channel effects. This engineered strain can be directly used as a functional live bacteria preparation for food and feed additives, with broad prospects for industrial application.

The application belongs to the technical field of sewage treatment, and provides a direct ammonia oxidation denitrification device, which comprises biological filler, an aerobic reaction tank, a stirrer, aeration stones, a first water outlet, a second water inlet, a backflow water outlet, a sludge backflow pump, a backflow water inlet, a membrane tank and an MBR membrane assembly; the aerobic reaction tank is internally provided with biological filler and a stirrer; the bottom of the aerobic reaction tank is provided with aeration stones; the aeration stones are connected with an air pump; the top of the membrane tank is provided with a water inlet; the bottom of the membrane tank is provided with a backflow water outlet; the middle of the membrane tank is provided with an MBR membrane assembly; the biological filler is provided with a biological membrane of Alcaligenes sp. HO-1; and the filter diameter of the MBR membrane assembly is not greater than 0.20 microns. The sewage / wastewater biological denitrification device and process based on direct ammonia oxidation provided by the application have high denitrification efficiency and simple denitrification process.

This invention discloses a chemoautotrophic alkaloid-producing strain of *Alcaligenes rocheborella*, its construction method, and its application. The strain is based on the chemoautotrophic microorganism *Alcaligenes rocheborella* H16 (… Cupriavidus necator Using H16 as the starting strain, the poly-3-hydroxybutyrate (PHB) synthesis pathway of the strain was first knocked out, and then a strain from Rhodotorula glutinis (H16) was introduced. Rhodotorula glutinis ) tyrosine ammonia lyase gene tal A basic engineered strain capable of producing p-coumaric acid was constructed. Subsequently, the throughput of the p-coumaric acid synthesis pathway in the engineered strain was enhanced by overexpressing the endogenous 3-deoxy-7-phosphate heptanulate synthase gene aroG1 and the branching acid synthase gene aroC, and by increasing the tal copy number. Further enhancement was achieved by introducing strains from *Escherichia coli* (…). Escherichia coli By improving the supply of cofactors through the NAD(P) transhydrogenase gene pntAB, a strain of Alcaligenes roximatelye that can synthesize p-coumaric acid using CO2 was finally obtained.

The invention relates to a preparation method of freeze-thaw-resistant immobilized particles and application of the freeze-thaw-resistant immobilized particles to degradation of enrofloxacin under freeze-thaw conditions. The immobilized particles mainly comprise sodium alginate, sodium pyrophosphate, sodium tripolyphosphate, sodium hexametaphosphate, cane sugar, zeolite and charcoal. When the enrofloxacin is degraded, an embedded bacterial strain is alcaligenes ammonoxydans, is named as Y-ENR, is preserved in the China General Microbiological Culture Collection Center, the address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No: 25659, and the sequence of the bacterial strain is as shown in SEQ ID No.1. The particles are high in mechanical strength, remarkable in freeze-thaw resistance and easy to recycle, and the effect of degrading enrofloxacin by the Y-ENR strain under the freeze-thaw condition can be remarkably improved. According to the invention, a treatment environment is expanded to a freeze-thaw condition, and support is provided for construction of an antibiotic biodegradation technology in winter cold regions in northern China.

The invention relates to alcaligenes faecalis AF12 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The alcaligenes faecalis AF12 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.37190, the preservation date is December 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides application of the alcaligenes faecalis AF12 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The alcaligenes faecalis AF12 provided by the invention can be applied to any plant species or plant habitat, realizes multiple effects of plant growth promotion, prevention and control of plant fungal diseases and bacterial diseases and prevention and control of plant root-knot nematode, and has extremely high application value and popularization prospect in the field of plant cultivation.

The invention relates to the technical field of flocculant development, in particular to a bio-based flocculant and a preparation method thereof.The bio-based flocculant comprises 15%-25% of cationic polyacrylamide, 23%-57% of polymeric silicate aluminum ferric sulfate, 6%-10% of heavy metal composite adsorption aid powder, 13%-23% of a magnetic adsorption aid, 7%-13% of composite bacterial powder, 2%-5% of a microbial deodorant, 4%-9% of a microbial oxygen aid and 2%-16% of an excipient. The heavy metal composite adsorption assistant powder comprises stenotrophomonas maltophilia, providencia alcaligenes and a mixture of metallophilic bacteria and lysinibacillus, the composite bacterial powder is a mixture of agaricus blazei hyphae, lentinus edodes mushroom residues, flammulina velutipes mushroom residues and oyster mushroom residues, and the microbial oxygen assistant is a mixture of cyanobacteria chlorophycocyanobacteria and zoogloea. On one hand, the flocculation speed and the decomposition speed of heavy metal impurities in sewage can be effectively increased, on the other hand, magnetic adsorption and rapid flocculation of the heavy metal impurities in the sewage can be achieved, and meanwhile, the flocculant can also achieve increase of the oxygen content of the sewage, so that the oxygen exposure purification efficiency during sewage flocculation is effectively improved.

This invention relates to the field of medical data processing technology, specifically to a diagnostic method for postmenopausal osteoporosis (PMOP) based on oral salivary bacteria. The method includes: collecting saliva samples from the subject in the morning on an empty stomach and labeling the subject's menopausal years; obtaining the relative abundance of Lactobacillus and Streptococcus, the concentrations of propionic acid and acetic acid, and the pH value; determining the corrected bone mineral density (BMD) correlation value using a diagnostic model; and outputting suggestions based on the BMD correlation value. This invention utilizes the principle that the decline in estrogen levels in postmenopausal women leads to an imbalance in the salivary flora, with an increase in acid-producing bacteria leading to increased acetic acid secretion and a decrease in alkali-producing bacteria leading to decreased propionic acid secretion. These two factors synergistically exacerbate osteogenic inhibition and increased bone resorption. Furthermore, since most saliva is partially absorbed after swallowing, the long-term accumulation of propionic acid and acetic acid can interfere with bone metabolism through the circulatory system, thus providing a preliminary diagnosis of PMOP. By collecting only saliva samples, without blood collection or radiation, the procedure is simple, painless, and provides physicians with preliminary diagnostic suggestions.

The invention discloses a complex microbial inoculant with a cellulose-protein two-component degradation function as well as a preparation method and application of the complex microbial inoculant. According to the present invention, the active components of the composite bacterial agent comprise Acinetobacter baumannii (Acinetobacter baumannii) and Providencia alcaligenes (Providencia alcaligenes), and the active components of the composite bacterial agent comprise a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain, a bacillus amyloliquefaciens strain and a bacillus amyloliquefaciens strain, the number of the acinetobacter baumannii in the China Industrial Microbiological Culture Collection Center is CICC 22933; the providencia alcaligenes is numbered as CICC (China Industrial Microbiological Culture Collection Center) 25067 in the China Industrial Microbiological Culture Collection Center). The strains in the complex microbial inoculant do not have antagonism, and the complex microbial inoculant shows a remarkable synergistic effect when degrading cellulose and protein, so that the waste conversion efficiency and the yield of hermetia illucens can be synchronously improved, the environmental pollution is reduced, and a new technical support is provided for high-value utilization of agricultural wastes.