26 results about "Phenol degradation" patented technology

The invention belongs to the technical field of microbial fuel cells, and particularly relates to a microbial fuel cell, a carbon-based anode and a preparation method of the carbon-based anode. Preparing a polyaniline / dopamine modifier from an aniline monomer and dopamine hydrochloride, and modifying the polyaniline / dopamine modifier on a carbon felt electrode to form a PANI-DA / CF anode; the preparation method comprises the following steps: preparing flower-like Fe2O3 nanoparticles by adopting a hydrothermal method, and electrochemically depositing the flower-like Fe2O3 nanoparticles on a PANI-DA / CF electrode to prepare a Fe2O3 / PANI-DA / CF anode; according to the microbial fuel cell disclosed by the invention, the maximum power density of the phenol wastewater anolyte reaches 4594 + / -97 mW / m < 2 > and is improved by 83.1% compared with that of a carbon felt anode MFC (Microbial Fuel Cell); after operation for 96 hours, the phenol degradation rate reaches 99.4%; and the electricity generation performance is still kept stable after three months of operation. The Fe2O3 / PANI-DA / CF anode provided by the invention improves the power density output of the microbial fuel cell and the wastewater treatment efficiency.

The microalgae phenol degradation and carbon fixation technology aims to provide a method for improving key enzymes to promote euglena to degrade phenol and enhance carbon fixation. The method comprises the following steps: adjusting the gene expression amount of three key enzymes, H+ transport ATPase, pyruvate decarboxylase and isocitrate dehydrogenase in euglena cells by performing phenol concentration gradient domestication on the euglena cells; performing large-scale culture growth on the euglena domesticated strains, adding appropriate phenol in the culture medium, and continuously feeding the gas containing CO2; calculating the carbon fixation rate of the euglena by measuring the carbon element content in the biomass of the algal liquid during the culture process; after the culture is completed, the euglena polysaccharide content in the unit volume of the algal liquid is measured, and the phenol content in the algal liquid is measured to calculate the phenol degradation rate. The euglena cells are domesticated by using the phenol concentration gradient, the gene expression amount of the key enzymes is improved by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon fixation rate is improved, and the polysaccharide content in the cells is also improved.

The application discloses a preparation method of a ferroferric oxide electrode material and application of the ferroferric oxide electrode material in an electrode, a device and a method for photoelectrocatalytic degradation of phenol. The electrode is prepared by in-situ adhering a layer of uniform iron-containing intermediate on the surface of a metal mesh through thermal decomposition of an iron-containing compound, and converting the layer of the iron-containing intermediate into ferroferric oxide through heat treatment. The obtained electrode can realize photoelectrocatalytic complete degradation of water-phase phenol under irradiation of ultraviolet light, and the performance is far higher than that of a ferroferric oxide electrode based on a metal sheet and a ferroferric oxide electrode based on FTO under the same conditions. The preparation method has mild conditions, a simple and controllable process, and high raw material utilization rate; the prepared electrode is low in price, environment-friendly and non-toxic, uniform and dense in the film layer, high in catalyst loading per unit area, and can simultaneously improve the two problems of poor light condition and low material exchange rate existing in a photoelectrocatalytic water-phase phenol degradation system, and has high practical application value.

The invention discloses a Fe-modified TiO2 hollow microsphere photo-Fenton catalyst as well as a preparation method and application thereof, and relates to the technical field of catalyst preparation, and the preparation method comprises the following steps: preparing TiO2 microspheres with hollow structures from hexadecyl trimethyl ammonium bromide, absolute methanol, deionized water and tetrabutyl titanate; the method comprises the following steps: mixing ferrocene and 0.25 C-TiO2 powder, putting the mixture into a quartz tube, putting the quartz tube into a muffle furnace, heating and pyrolyzing according to a two-stage program, alternately washing black powder with absolute ethyl alcohol and deionized water after the pyrolysis is finished, and drying to obtain a sample CT-5Fe; the preparation method comprises the following steps: dispersing CT-5Fe in an H2O2 solution, carrying out a water bath reaction, collecting a gray product, carrying out centrifugal operation, washing, and drying to obtain the Fe-modified TiO2 hollow microsphere photo-Fenton catalyst, namely CT-5Fe-HO. According to the invention, a photocatalysis-Fenton-like coupling system for phenol degradation is constructed, the PMS adsorption and activation capability is enhanced, high activity and good cycle stability are maintained in a wide pH range and in a complex water body, and a solution is provided for water body pollution treatment.

The invention discloses a high-efficiency electrocatalytic oxidation electrode production process, which relates to the technical field of electrochemical catalytic materials, and comprises the following steps: pretreatment of an electrode substrate, construction of an intermediate conductive layer, preparation of an active component precursor solution, loading of active components, and heat treatment for film formation. According to the invention, the oxygen-rich vacancy structure is formed through cooperation of multiple metals, the SnO2 / TiO2 intermediate conductive layer is constructed, and precise heat treatment is carried out at 450 DEG C, so that the phenol degradation current density reaches 25mA / cm, and the overpotential of electrolyzed water for oxygen production is 280mV; the non-noble metal and the graphite substrate are adopted, the cost is only 5%-20% of that of a noble metal electrode, the attenuation is less than 10% when the electrode operates for 200 hours in an environment with the pH value of 2, the process is simple and convenient, the electrode is adaptive to multiple scenes such as titanium / graphite substrate and wastewater treatment, and the existing electrode performance and cost bottlenecks are broken through.

The application provides a phenol degradation method, comprising the following steps: inoculating Cupriavidus basellens in a first culture medium with a phenol concentration of 200-800 mg / L, culturing at a temperature of 26-36 DEG C under a rotating speed of 120-150 rpm, and realizing phenol degradation through lysis growth of the Cupriavidus basellens; wherein the initial volume ratio of the Cupriavidus basellens to the first culture medium is 5-10%, and the pH value of the first culture medium is 5-11. The application can efficiently and highly degrade phenol, has a remarkable effect, and is worth promoting.

A preparation method and application of a zinc oxide-coated copper plasma composite photocatalytic material for phenol degradation, which relates to a preparation method and application of a photocatalytic material. The present invention aims to solve the problem that the existing semiconductor catalyst has a competitive relationship between excitons and free charge carriers, resulting in a single deficiency of ROS generated by the semiconductor in the photocatalytic system. The present invention uses ZnO, a direct bandgap semiconductor with a good exciton dissociation effect, as a substrate, and introduces bulk oxygen vacancies and elemental copper with a plasmon resonance effect in situ to precisely control the balance between excitons and photogenerated carriers, thereby achieving the generation of enriched oxidative active species in the photocatalytic system. Method: 1. Material mixing; 2. Hydrothermal reaction. Application: It is used as a photocatalyst for the photocatalytic degradation of phenol. The present invention discloses the preparation and application of a zinc oxide-coated copper plasma composite photocatalytic material for phenol degradation.

The invention relates to a polyurethane adsorption-gel embedding microorganism composite immobilization method and application thereof, and belongs to the technical field of coking wastewater treatment and microorganisms. Polyurethane sponge is immersed into a mixed solution containing phenol degrading bacteria and sodium alginate, after sufficient adsorption, the polyurethane sponge is transferred into a calcium chloride-boric acid mixed solution for cross-linking curing, and finally the organic gel composite carrier with a porous structure is formed. The immobilized carrier can significantly enhance the resistance of phenol degrading bacteria to environmental stress, and improve the adaptability and stability of the phenol degrading bacteria under different water quality conditions. Meanwhile, the carrier is simple and convenient in preparation process and low in cost, has good reusability, is suitable for efficient immobilization of microorganisms in the water treatment process, and has wide popularization and application prospects.

The application provides a screening, culture and application method of a low-temperature and high-salt resistant phenol degradation strain, and an Alcaligenes sp. HZ-F-002 which is separated and purified by the application and has a preservation number of CGMCC NO.32175, can resist high-concentration phenol, can grow and degrade phenol in a temperature range of 10-35 DEG C, can resist a high-salt environment and has wide-area pH adaptability, can take phenol as the only energy source in a degradation process, and does not need to add extra carbon source and nitrogen source. The strain is suitable for phenol degradation of high-salt, high-phenol and low-temperature wastewater. Through optimization of fermentation culture conditions, high-density culture of the Alcaligenes sp. is realized, and then a biological bacterial agent is prepared, so that the goal of low cost, high efficiency and pollution-free degradation of phenol in on-site application can be achieved.

The invention provides a screening, culturing and application method of a low-temperature-resistant and high-salt-resistant phenol degrading strain, the preservation number of the separated and purified alcaligenes sp. HZ-F-002 is CGMCC NO.32175, and the alcaligenes sp. HZ-F-002 can tolerate high-concentration phenol, can well grow and degrade phenol in the temperature range of 10-35 DEG C, can tolerate a high-salt environment, has wide-area pH adaptability, and can be used for preparing high-concentration phenol degrading bacteria. In the degradation process, phenol can be used as a unique energy source, and a carbon source and a nitrogen source do not need to be additionally added. The strain is suitable for phenol degradation of high-salt, high-phenol and low-temperature wastewater. And by optimizing fermentation culture conditions, high-density culture of the alcaligenes is realized, and the alcaligenes are further prepared into a biological agent, so that the aim of degrading phenol at low cost, high efficiency and no pollution during field application can be achieved.

The invention relates to an iron-based integral mesh catalyst and a preparation method thereof. The preparation method comprises the following steps: firstly, cleaning two commercial iron mesh substrates, and putting the cleaned commercial iron mesh substrates into an electrolytic tank to serve as a working electrode and a counter electrode; then an electrolyte is prepared, the electrolyte is one of cobalt nitrate, manganese nitrate or zinc nitrate, and the covering areas of the electrolyte and the substrate are the same; then, a chronopotentiometric method is adopted for treatment, and active components are uniformly dispersed and firmly loaded on the surface of the iron net in a nanostructure form through electrochemical polarization to form a metal oxide layer with a mixed valence state, so that electron transfer and hydroxyl free radical generation are promoted in the catalysis process; and finally, cleaning, drying and roasting the treated substrate to obtain the catalyst. Compared with the prior art, the electrochemical synthesis method is simple, easy to operate and high in preparation efficiency, a large amount of waste liquid containing metal ions cannot be generated, and the prepared catalyst has very high ozone catalytic oxidation phenol degradation activity and stability.

The invention discloses an acid-resistant and alkali-resistant efficient phenol degrading bacterium and application thereof, and belongs to the technical field of environmental microorganisms. Through identification, the screened strain G0-1 is Acinetobacter bereziniae, the strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20251392, and the preservation date is June 16, 2025. The strain G0-1 can take phenol as the sole carbon source and energy to carry out metabolic activity, 500 mg / L of phenol can be completely degraded within 12 hours, and the highest tolerance concentration can reach 1400 mg / L. It is worthy of noting that the strain can keep stable phenol degradation activity in a wide range of pH 4-10. The G0-1 strain obtained by the invention shows remarkable advantages in the aspect of treating phenol-containing wastewater, has the characteristics of high treatment efficiency, low cost, no secondary pollution, strong environment tolerance and the like, and has important theoretical and application values in the field of environmental pollution treatment.

The present invention discloses an acid-resistant and alkali-resistant high-efficiency phenol-degrading bacterium and its application, belonging to the field of environmental microbiology technology. The screened strain G0-1 ​​was identified as Acinetobacter berezzii ( Acinetobacter bereziniae ), the strain has been deposited in the China Center for Type Culture Collection with the deposit number CCTCC NO: M20251392 and the deposit date is June 16, 2025. Strain G0‑1 can use phenol as the sole carbon source and energy source for metabolic activities, and can completely degrade 500 mg / L of phenol within 12 h, and its maximum tolerance concentration can reach 1400 mg / L. It is worth noting that the strain can maintain stable phenol degradation activity over a wide range of pH 4‑10. The G0‑1 strain obtained by the present invention shows significant advantages in treating phenol-containing wastewater, including high treatment efficiency, low cost, no secondary pollution and strong environmental tolerance, and has important theoretical and application value in the field of environmental pollution control.

The tungsten-doped cobalt sulfide composite material provided by the invention takes Co3S4 as a matrix and is loaded with WS2; the single Co-based catalyst is mainly used for degrading pollutants by activating monopersulfate through Co < 2 + > to generate free radicals with strong oxidizing property and simultaneously generating Co < 3 + >, the Co < 3 + > can also react with monopersulfate to be converted into Co < 2 + >, but the conversion rate is relatively slow, which is a speed limiting step of the reaction; wS2 is doped into Co3S4, so that Co < 3 + > in a material matrix can be accelerated to be converted into Co < 2 + >, and Co < 3 + > can be accelerated to be converted into Co < 2 + > in a reaction process; further, the removal rate of the composite material for activating the monopersulfate to degrade the phenol is improved.

The invention relates to a carbon sequestration optimization method for synthesizing PHA (polyhydroxyalkanoate) from phenolic wastewater, which comprises the following steps: setting three operation modes of batch, fed-batch and continuous operation, and constructing and simulating toxic industrial phenolic wastewater by using phenol as a model substrate; under the three modes, the reactor is operated with comparable process parameters, the reaction process of substrate phenol degradation and product PHA synthesis is recorded, and the carbon flow (organic carbon direction) between the substrate and the product is calculated; by changing technological parameters such as the phenol concentration in inlet water and the food / micro-organism ratio (F / M ratio which can be changed by changing the amount of a substrate flowing into the system and / or changing the amount of microorganisms in the system) in the system and the like, results are compared to obtain a better carbon sequestration effect. Compared with the prior art, the carbon sequestration potential and efficiency of taking the green plastic PHA as the carbon sink under different flow patterns and different operation parameters are systematically compared aiming at toxic and inhibitory substrates, and a powerful method and tool are provided for carbon reduction.

The present invention discloses the application of Siamese Bacillus L5 in phenol degradation; the bacterium has been deposited in China Center for Type Culture Collection on November 1, 2019, with the deposit number of CCTCC M 2019885. Strain L5 can efficiently degrade phenolic compounds such as phenol, catechol, resorcinol, 3,5-dimethylphenol, 2-methoxyphenol and 2,6-dimethoxyphenol. The degradation bacterial agent prepared by mixing the fermentation liquid of strain L5 with a porous glass carrier can further improve the degradation rate, and the strain can be applied to the degradation of phenolic compounds in the environment by direct addition. The preparation method of the bacterial agent is simple, convenient and efficient to use, and can safely and quickly degrade the phenol remaining in water and soil, and has a good application prospect in the pollution control of phenolic compounds such as phenol.

The invention discloses an application of a sludge-based catalyst in phenol degradation. The sludge-based catalyst is prepared by the following steps: mixing crushed dry-based sludge with water, reacting at 190-210 DEG C for 20-40 minutes, carrying out solid-liquid separation, drying the solid, and firing the dried material at 700-750 DEG C in a nitrogen atmosphere for 200-250 minutes to obtain sludge carbon; the method comprises the following steps: mixing crushed pyrite slag with a hydrochloric acid solution with the mass concentration of 15-30%, reacting at 60-95 DEG C for 1-4 hours, carrying out solid-liquid separation, and introducing oxygen or air into the liquid to oxidize for 24 hours, so as to prepare an impregnation liquid; adding sludge carbon and oxalic acid into the steeping liquor, stirring and reacting for 4-4.5 hours, carrying out solid-liquid separation, drying a solid, and calcining in a nitrogen atmosphere, according to the method, sewage is purified while sludge is treated, efficient recycling of resources is promoted, and the concept of green and sustainable development is met.

The application discloses a 3D bioprinting bacteria-algae symbiotic engineering living material and a preparation method and application thereof. The method takes chlorella as a biological oxygen supply source, takes alkali-producing bacteria as phenol-degrading bacteria, takes sodium alginate and carboxymethyl cellulose as carriers, and respectively prepares bacterial bio-ink and algal bio-ink added with silicon dioxide, so as to build bacteria-algae symbiotic engineering living materials with stripe and layer configurations through a 3D bioprinting mechanism. The bacteria-algae symbiotic engineering living material realizes three-dimensional accurate arrangement and functional partition of bacteria and algae, and exhibits excellent degradation performance and photosynthetic performance under different phenol concentrations, and has application prospects in the biological degradation treatment of organic pollutants.

The present invention discloses a phosphorus-removing and phenol-degrading bacterium and its application. This bacterium is a new strain of Zobellella sp., with the strain number S101 and the preservation number CGMCC No. 29317 at the General Microbiology Center of the China Committee for Culture Collection of Microorganisms. This bacterium can perform heterotrophic nitrification and phosphorus removal, and can degrade phenol, and will have broad application prospects in aspects such as environmental governance.

The application discloses a method for preparing carbon dots by electrochemical coupling of an oxidant and application thereof. The method is to prepare carbon quantum dots by electrolyzing a graphite rod in an electrolyte solution containing hydrogen peroxide. The carbon dots synthesized by the method have the characteristics of simple and economical synthesis process, high yield, good performance, high content of surface functional groups and the like. When the carbon dots are applied to a Fenton-like process, better phenol degradation effect can be achieved in a wider pH range.

The invention belongs to the field of electrocatalytic oxidation, and provides a preparation method of a doped DSA electrode. The method comprises the following steps: pretreating a DSA matrix; a tin, lead and rare earth element alloy layer is electrically deposited; and preparing the porous doped oxide through anodic oxidation. The process is simple, coating or sintering is not needed, and steps are reduced by 50% or above; a water-based solution is used in the whole process, and organic solvent pollution is avoided; the electrode bonding force is good, and the bonding strength of the alloy layer and the substrate is larger than 20 MPa; cost is low, precious metal consumption is reduced by 80%, and raw material cost is reduced by 40%; the catalytic efficiency is high, the specific surface area reaches 50-100 m / g, and the phenol degradation rate exceeds 90%; and the service life is long, and stable operation is carried out for more than 1000 hours in an acceleration test. The method is suitable for the fields of industrial wastewater treatment, electrochemical synthesis and the like.

This invention belongs to the field of biological wastewater treatment, specifically relating to a composite microbial agent, its preparation method, and its application. By mass percentage, it comprises 60%-80% bioactive agent and 20%-40% activity protectant. The bioactive agent includes 66.58%-86.58% of *Haloxylon ammodendron* strains, 7.85%-22.85% of *Hymenobacter* strains, and 5.57%-25.57% of *Thiobacillus* strains. The total viable count of the composite microbial agent is not less than 1 × 10⁻⁶. 9 The compound microbial agent was added to distilled water and cultured under anaerobic shaking conditions to obtain a revived bacterial solution. The supernatant of the revived bacterial solution was mixed with the wastewater to be treated at a volume ratio of 1-10:250, and 100-250 mg / L of a compatible solute was added. The wastewater was then treated under anaerobic conditions. After one week of treatment of low C / N ratio, high-salt phenol wastewater using this compound microbial agent, the denitrification rate remained above 95%, and the phenol degradation rate remained above 80%. This effectively solved the problems of low denitrification efficiency and difficulty in removing phenolic compounds from high-salt phenol wastewater, and the process was simple and low-cost.

The invention provides an engineering bacterium J9U-PP for co-degrading phenol and p-nitrophenol as well as a construction method and application of the engineering bacterium J9U-PP, and belongs to the technical field of genetic engineering. The engineering bacterium J9U-PP is prepared by inserting pheA1, pheA2, pnpA, pnpB, pnpC, pnpD, pnpE and pnpF into the Halomonas craagoides J9U. The engineering bacterium J9U-PP not only can stably express a phenol degradation gene and a PNP degradation gene, but also can efficiently degrade one or more of phenol, catechol, hydroquinone and p-nitrophenol, and when the engineering bacterium J9U-PP singly degrades samples containing degraded phenol, catechol, hydroquinone and p-nitrophenol, the degradation efficiency reaches up to 100%. And the engineering bacterium J9U-PP shows excellent effect and competitiveness of degrading phenol and p-nitrophenol under a high-salt condition, and has good potential in in-situ bioremediation of a polluted environment.

The invention relates to the technical field of nano catalytic materials, and particularly discloses preparation and application of a monatomic catalyst for phenol degradation. According to the catalyst, nitrogen-doped porous carbon serves as a carrier, transition metal atoms (Fe / Co / Mn / Cu) are dispersed on the carrier in an atomic scale mode through an M-N4 coordination structure, and the metal loading capacity is 0.5 wt%-5.0 wt%; the preparation method comprises the following steps: (1) metal acetylacetonate / 2-methylimidazole / melamine coordination self-assembly; (2) carrying out gradient pyrolysis under nitrogen; (3) metal particles are selectively removed through acid pickling; when the catalyst is used for degrading 10-200 mg / L phenol in a persulfate activation system under a neutral condition for 30 minutes, the removal rate is greater than or equal to 92%, and the activity retention rate is greater than or equal to 85% after the catalyst is recycled for 5 times; the method is suitable for advanced treatment of industrial wastewater, and has the advantages of high atom utilization rate, wide pH adaptability and low cost.

The invention relates to a method for treating high-nitrogen phenolic wastewater by coupling a microbial electrolysis cell with anaerobic ammonia oxidation, which comprises the following steps: S1, constructing a microbial electrolysis cell reactor with a single reaction chamber, placing an anode and a cathode of the reactor in the same reaction chamber, and inoculating activated sludge containing anaerobic ammonia oxidation bacteria in the reactor; s2, performing toxicity domestication on the activated sludge; s3, high-nitrogen phenolic wastewater is introduced into the reactor, voltage is applied to the anode and the cathode through an adjustable direct-current stabilized power supply, and denitrification and phenol degradation are synchronously achieved through coupling of the microbial electrolysis cell and the anaerobic ammonia oxidation reaction. Compared with the prior art, the method has the advantages that the single-reaction-chamber microbial electrolytic tank is coupled with the anaerobic ammonium oxidation process, targeted phenol toxicity domestication is carried out on anaerobic ammonium oxidation bacterium activated sludge, and voltage is applied to enhance the microbial activity, so that high-nitrogen phenol-containing wastewater denitrification and phenol degradation are synchronously carried out, and an external carbon source and aeration are not needed; the domestication period is short, and the pollutant removal efficiency is high.

The invention relates to an optimization method for repairing an organic pollution site. The optimization method comprises the following steps: classifying organic pollution soil according to a solvent extraction method; signals of the persistent free radicals are detected through an electron paramagnetic resonance spectrometer, characteristic parameters of the signals are counted, and the oxidation-reduction capacity of the four soil samples is measured through cyclic voltammetry and an electrochemical impedance spectrum; evaluating the action efficiency of the repairing agent; freeze-drying the reacted soil sample, performing EPR signal detection, and counting characteristic parameters of the soil sample; calculating the theoretical adding amount of H2O2 according to the model; applying the obtained theoretical dosage of H2O2 to a phenol degradation system, and carrying out a phenol degradation experiment; regularly sampling and measuring the phenol content. By detecting the electronic structures, functional groups and reaction activities of PFRs in different occurrence states, the quantitative relationship between the dosage of the repairing agent and the repairing efficiency is established, and the problems of excessive repairing and uncontrollable repairing effect of the repairing agent in the prior art are solved.