13 results about "Phenol degradation" patented technology

The microalgae phenol degradation and carbon fixation technology aims to provide a method for improving key enzymes to promote euglena to degrade phenol and enhance carbon fixation. The method comprises the following steps: adjusting the gene expression amount of three key enzymes, H+ transport ATPase, pyruvate decarboxylase and isocitrate dehydrogenase in euglena cells by performing phenol concentration gradient domestication on the euglena cells; performing large-scale culture growth on the euglena domesticated strains, adding appropriate phenol in the culture medium, and continuously feeding the gas containing CO2; calculating the carbon fixation rate of the euglena by measuring the carbon element content in the biomass of the algal liquid during the culture process; after the culture is completed, the euglena polysaccharide content in the unit volume of the algal liquid is measured, and the phenol content in the algal liquid is measured to calculate the phenol degradation rate. The euglena cells are domesticated by using the phenol concentration gradient, the gene expression amount of the key enzymes is improved by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon fixation rate is improved, and the polysaccharide content in the cells is also improved.

The invention discloses a Fe-modified TiO2 hollow microsphere photo-Fenton catalyst as well as a preparation method and application thereof, and relates to the technical field of catalyst preparation, and the preparation method comprises the following steps: preparing TiO2 microspheres with hollow structures from hexadecyl trimethyl ammonium bromide, absolute methanol, deionized water and tetrabutyl titanate; the method comprises the following steps: mixing ferrocene and 0.25 C-TiO2 powder, putting the mixture into a quartz tube, putting the quartz tube into a muffle furnace, heating and pyrolyzing according to a two-stage program, alternately washing black powder with absolute ethyl alcohol and deionized water after the pyrolysis is finished, and drying to obtain a sample CT-5Fe; the preparation method comprises the following steps: dispersing CT-5Fe in an H2O2 solution, carrying out a water bath reaction, collecting a gray product, carrying out centrifugal operation, washing, and drying to obtain the Fe-modified TiO2 hollow microsphere photo-Fenton catalyst, namely CT-5Fe-HO. According to the invention, a photocatalysis-Fenton-like coupling system for phenol degradation is constructed, the PMS adsorption and activation capability is enhanced, high activity and good cycle stability are maintained in a wide pH range and in a complex water body, and a solution is provided for water body pollution treatment.

The application provides a phenol degradation method, comprising the following steps: inoculating Cupriavidus basellens in a first culture medium with a phenol concentration of 200-800 mg / L, culturing at a temperature of 26-36 DEG C under a rotating speed of 120-150 rpm, and realizing phenol degradation through lysis growth of the Cupriavidus basellens; wherein the initial volume ratio of the Cupriavidus basellens to the first culture medium is 5-10%, and the pH value of the first culture medium is 5-11. The application can efficiently and highly degrade phenol, has a remarkable effect, and is worth promoting.

The invention relates to a polyurethane adsorption-gel embedding microorganism composite immobilization method and application thereof, and belongs to the technical field of coking wastewater treatment and microorganisms. Polyurethane sponge is immersed into a mixed solution containing phenol degrading bacteria and sodium alginate, after sufficient adsorption, the polyurethane sponge is transferred into a calcium chloride-boric acid mixed solution for cross-linking curing, and finally the organic gel composite carrier with a porous structure is formed. The immobilized carrier can significantly enhance the resistance of phenol degrading bacteria to environmental stress, and improve the adaptability and stability of the phenol degrading bacteria under different water quality conditions. Meanwhile, the carrier is simple and convenient in preparation process and low in cost, has good reusability, is suitable for efficient immobilization of microorganisms in the water treatment process, and has wide popularization and application prospects.

The application provides a screening, culture and application method of a low-temperature and high-salt resistant phenol degradation strain, and an Alcaligenes sp. HZ-F-002 which is separated and purified by the application and has a preservation number of CGMCC NO.32175, can resist high-concentration phenol, can grow and degrade phenol in a temperature range of 10-35 DEG C, can resist a high-salt environment and has wide-area pH adaptability, can take phenol as the only energy source in a degradation process, and does not need to add extra carbon source and nitrogen source. The strain is suitable for phenol degradation of high-salt, high-phenol and low-temperature wastewater. Through optimization of fermentation culture conditions, high-density culture of the Alcaligenes sp. is realized, and then a biological bacterial agent is prepared, so that the goal of low cost, high efficiency and pollution-free degradation of phenol in on-site application can be achieved.

The invention relates to an iron-based integral mesh catalyst and a preparation method thereof. The preparation method comprises the following steps: firstly, cleaning two commercial iron mesh substrates, and putting the cleaned commercial iron mesh substrates into an electrolytic tank to serve as a working electrode and a counter electrode; then an electrolyte is prepared, the electrolyte is one of cobalt nitrate, manganese nitrate or zinc nitrate, and the covering areas of the electrolyte and the substrate are the same; then, a chronopotentiometric method is adopted for treatment, and active components are uniformly dispersed and firmly loaded on the surface of the iron net in a nanostructure form through electrochemical polarization to form a metal oxide layer with a mixed valence state, so that electron transfer and hydroxyl free radical generation are promoted in the catalysis process; and finally, cleaning, drying and roasting the treated substrate to obtain the catalyst. Compared with the prior art, the electrochemical synthesis method is simple, easy to operate and high in preparation efficiency, a large amount of waste liquid containing metal ions cannot be generated, and the prepared catalyst has very high ozone catalytic oxidation phenol degradation activity and stability.

The invention relates to a carbon sequestration optimization method for synthesizing PHA (polyhydroxyalkanoate) from phenolic wastewater, which comprises the following steps: setting three operation modes of batch, fed-batch and continuous operation, and constructing and simulating toxic industrial phenolic wastewater by using phenol as a model substrate; under the three modes, the reactor is operated with comparable process parameters, the reaction process of substrate phenol degradation and product PHA synthesis is recorded, and the carbon flow (organic carbon direction) between the substrate and the product is calculated; by changing technological parameters such as the phenol concentration in inlet water and the food / micro-organism ratio (F / M ratio which can be changed by changing the amount of a substrate flowing into the system and / or changing the amount of microorganisms in the system) in the system and the like, results are compared to obtain a better carbon sequestration effect. Compared with the prior art, the carbon sequestration potential and efficiency of taking the green plastic PHA as the carbon sink under different flow patterns and different operation parameters are systematically compared aiming at toxic and inhibitory substrates, and a powerful method and tool are provided for carbon reduction.

The application discloses a 3D bioprinting bacteria-algae symbiotic engineering living material and a preparation method and application thereof. The method takes chlorella as a biological oxygen supply source, takes alkali-producing bacteria as phenol-degrading bacteria, takes sodium alginate and carboxymethyl cellulose as carriers, and respectively prepares bacterial bio-ink and algal bio-ink added with silicon dioxide, so as to build bacteria-algae symbiotic engineering living materials with stripe and layer configurations through a 3D bioprinting mechanism. The bacteria-algae symbiotic engineering living material realizes three-dimensional accurate arrangement and functional partition of bacteria and algae, and exhibits excellent degradation performance and photosynthetic performance under different phenol concentrations, and has application prospects in the biological degradation treatment of organic pollutants.

The application discloses a method for preparing carbon dots by electrochemical coupling of an oxidant and application thereof. The method is to prepare carbon quantum dots by electrolyzing a graphite rod in an electrolyte solution containing hydrogen peroxide. The carbon dots synthesized by the method have the characteristics of simple and economical synthesis process, high yield, good performance, high content of surface functional groups and the like. When the carbon dots are applied to a Fenton-like process, better phenol degradation effect can be achieved in a wider pH range.

The invention belongs to the field of electrocatalytic oxidation, and provides a preparation method of a doped DSA electrode. The method comprises the following steps: pretreating a DSA matrix; a tin, lead and rare earth element alloy layer is electrically deposited; and preparing the porous doped oxide through anodic oxidation. The process is simple, coating or sintering is not needed, and steps are reduced by 50% or above; a water-based solution is used in the whole process, and organic solvent pollution is avoided; the electrode bonding force is good, and the bonding strength of the alloy layer and the substrate is larger than 20 MPa; cost is low, precious metal consumption is reduced by 80%, and raw material cost is reduced by 40%; the catalytic efficiency is high, the specific surface area reaches 50-100 m / g, and the phenol degradation rate exceeds 90%; and the service life is long, and stable operation is carried out for more than 1000 hours in an acceleration test. The method is suitable for the fields of industrial wastewater treatment, electrochemical synthesis and the like.

This invention belongs to the field of biological wastewater treatment, specifically relating to a composite microbial agent, its preparation method, and its application. By mass percentage, it comprises 60%-80% bioactive agent and 20%-40% activity protectant. The bioactive agent includes 66.58%-86.58% of *Haloxylon ammodendron* strains, 7.85%-22.85% of *Hymenobacter* strains, and 5.57%-25.57% of *Thiobacillus* strains. The total viable count of the composite microbial agent is not less than 1 × 10⁻⁶. 9 The compound microbial agent was added to distilled water and cultured under anaerobic shaking conditions to obtain a revived bacterial solution. The supernatant of the revived bacterial solution was mixed with the wastewater to be treated at a volume ratio of 1-10:250, and 100-250 mg / L of a compatible solute was added. The wastewater was then treated under anaerobic conditions. After one week of treatment of low C / N ratio, high-salt phenol wastewater using this compound microbial agent, the denitrification rate remained above 95%, and the phenol degradation rate remained above 80%. This effectively solved the problems of low denitrification efficiency and difficulty in removing phenolic compounds from high-salt phenol wastewater, and the process was simple and low-cost.

The invention provides an engineering bacterium J9U-PP for co-degrading phenol and p-nitrophenol as well as a construction method and application of the engineering bacterium J9U-PP, and belongs to the technical field of genetic engineering. The engineering bacterium J9U-PP is prepared by inserting pheA1, pheA2, pnpA, pnpB, pnpC, pnpD, pnpE and pnpF into the Halomonas craagoides J9U. The engineering bacterium J9U-PP not only can stably express a phenol degradation gene and a PNP degradation gene, but also can efficiently degrade one or more of phenol, catechol, hydroquinone and p-nitrophenol, and when the engineering bacterium J9U-PP singly degrades samples containing degraded phenol, catechol, hydroquinone and p-nitrophenol, the degradation efficiency reaches up to 100%. And the engineering bacterium J9U-PP shows excellent effect and competitiveness of degrading phenol and p-nitrophenol under a high-salt condition, and has good potential in in-situ bioremediation of a polluted environment.

The invention relates to a method for treating high-nitrogen phenolic wastewater by coupling a microbial electrolysis cell with anaerobic ammonia oxidation, which comprises the following steps: S1, constructing a microbial electrolysis cell reactor with a single reaction chamber, placing an anode and a cathode of the reactor in the same reaction chamber, and inoculating activated sludge containing anaerobic ammonia oxidation bacteria in the reactor; s2, performing toxicity domestication on the activated sludge; s3, high-nitrogen phenolic wastewater is introduced into the reactor, voltage is applied to the anode and the cathode through an adjustable direct-current stabilized power supply, and denitrification and phenol degradation are synchronously achieved through coupling of the microbial electrolysis cell and the anaerobic ammonia oxidation reaction. Compared with the prior art, the method has the advantages that the single-reaction-chamber microbial electrolytic tank is coupled with the anaerobic ammonium oxidation process, targeted phenol toxicity domestication is carried out on anaerobic ammonium oxidation bacterium activated sludge, and voltage is applied to enhance the microbial activity, so that high-nitrogen phenol-containing wastewater denitrification and phenol degradation are synchronously carried out, and an external carbon source and aeration are not needed; the domestication period is short, and the pollutant removal efficiency is high.