The invention belongs to the technical field of biological
medicine, and particularly relates to a preparation method of a
probiotic hydrogel material and application of the
probiotic hydrogel material in treatment of acute radioactive
intestinal injury. In order to solve the clinical key problem of radioactive
intestinal injury, a novel biological material TG-CCS-coated DY802 of thiolated synchronously modified
chitosan hydrogel TG-CCS loaded with'localized high-response '
lactobacillus salivarius DY802 is constructed. The synthesis process of the material is synergistic and time-saving, has excellent
biological safety and
tumor inhibition experiment performance, and can comprehensively repair four barriers of the intestinal tract: ROS is removed, and
tight junction protein is protected to repair the
physical barrier; forming a hydrogel protective layer to reinforce the chemical barrier; the
flora diversity is improved, and the
prebiotic effect is exerted to repair the biological barrier; the
sphingomyelin metabolism and
ceramide abundance are regulated and controlled, the abnormal infiltration of NK cells is inhibited, and the
immune barrier is remodeled. Therefore, the
probiotic hydrogel material disclosed by the invention is clear in treatment mechanism and remarkable in clinical transformation potential and application value.