94 results about "Aerobic bacterium" patented technology

The invention relates to the field of wastewater treatment, and relates to a biological degradation method of nitrogen-containing heterocyclic compound wastewater. An oxidation state compound is added in the nitrogen containing heterocyclic compound wastewater, an anaerobic environment is formed by micro aeration, and mixed electron acceptors of dissolved oxygen and the oxidation state compound simultaneously exist in the system; an upflow biological aerated filter is used as a main reaction device, an optimal degradation effect of nitrogen-containing heterocyclic compounds is achieved according to different components and concentrations of the nitrogen-containing heterocyclic compounds, the pH is controlled to 7.0-7.5, the concentration of the dissolved oxygen is controlled to 0.05-0.3 mg/L (water discharged from the upper part of the filter), and the range of the oxidation-reduction potential is controlled to 150-50 mv; under an anaerobic aeration condition, with the nitrogen-containing heterocyclic compounds as electron donors and oxygen and oxygen-containing compounds as electron acceptors, under the combined effects of micro aerobic bacteria, facultative anaerobic bacteria, aerobic/anoxic denitrifying bacteria and the like, the nitrogen-containing heterocyclic compounds are completely degraded into carbon dioxide and water.

The invention provides a preparation method of fruit ferment. The preparation method comprises the following steps: carrying out anaerobic fermentation by inoculating anaerobic bacterium strains, filtering the obtained fermentation products, saving the filtrate (A), keeping on inoculating aerobic bacterium strains to peels and dregs of fruits to carry out aerobic fermentation for 5 to 15 days, then adding liquor, keeping on fermentation for 5 to 10 days, then filtering the fermentation products, condensing the filtrates, then merging the condensed filtrates, adding molasses, inoculating beneficial bacteria to the mixed filtrate, and sealing the mixed filtrate for 10 to 20 days to obtain the environment-friendly ferment. The preparation method can effectively avoid the mildew of fruit peels during the fermentation process, thus the content of nitrite is reduced, the obtained ferment prepared from fruit peels is healthy and unique, at the same time, the ferment content is increased, the function of the ferment is improved, moreover, the wastes (fruit peels) are converted into valuable resources, and the utilization of wastes is promoted.

The invention discloses aerobic bacterium-anaerobic bacterium composite microbe liquid inocula and a preparation method thereof. The composite microbe liquid inocula comprise six liquid aerobic microbial agents and four liquid anaerobic microbial agents. The liquid aerobic microbial agents comprise a bacillus megaterium agent, a liquid Azotobacter chroococcum agent, a liquid bacillus mucilaginosus agent, a liquid streptomyces microflavus agent, a liquid bacillus subtilis agent and a liquid bacillus licheniformis agent. The liquid anaerobic microbial agents comprise liquid photosynthetic bacteria, a liquid lactobacillus plantarum agent, a liquid lactobacillus acidophilus agent and a liquid lactobacillus delbrueckii agent. The preparation method comprises culturing six types of aerobic bacteria to obtain six liquid aerobic microbial agents, culturing four types of anaerobic bacteria to obtain four mixed liquid anaerobic microbial agents, and injecting the six liquid aerobic microbial agents into the mixed liquid anaerobic microbial agents to obtain the composite microbe liquid inocula. The composite microbe liquid inocula have a quality guarantee period for one year or more at the normal temperature and have functions of nitrogen fixation, phosphate dissolution, potassium release, disease prevention and soil improvement.

The invention belongs to the technical field of sewage treatment and discloses a broad spectrum biological agent for treating black and odorous water. The broad spectrum biological agent comprises a compound microbial community and a biological complex enzyme preparation, wherein the compound microbial community is composed of different anaerobic bacteria and aerobiotic bacteria. The broad spectrum biological agent for treating the black and odorous water provided by the invention has the beneficial effects that the problem of the black and odorous water resulted from pollution is quickly and effectively solved, the water quality is obviously improved and the ecological environment and ecological system of water can be quickly recovered.

The invention provides a garbage enzyme preparation method utilizing fruit peels. The preparation method comprises the following steps: the fruit peels are inoculated with an anaerobic bacterium for 10 to 20 days of anaerobic fermentation, and a first filtrate is reserved after filtering of the obtained product; fruit peel dregs are then inoculated with an aerobic bacterium for 5 to 15 days of aerobic fermentation, the fermentation continues for 5 to 10 days after white wine is added, and the product is then filtered; filtrates obtained through the two filtering processes are respectively concentrated and then mixed, molasses is added, the mixture is then inoculated with a beneficial bacterium, and garbage enzyme is obtained after 10 to 20 days of sealed storage. The garbage enzyme preparation method provided by the invention has the advantages that the effects of mildew of the fruit peels during fermentation are effectively avoided, so as to reduce the nitrite content, and ensure that the prepared fruit peel enzyme is healthy and unique; meanwhile, the enzyme content in the garbage enzyme is further increased, so as to improve the functional effects of the garbage enzyme; the fruit peels are used as raw materials, so as to achieve the effect of turning waste into wealth, and improve the waste utilization rate.

Provided is an isolated novel Gram-positive non-motile, non-spore-forming, aerobic bacterium designated Streptomyces scopuloiridis, wherein the bacterium produces a bacteriocin that kills or inhibits the growth of Gram-positive or Gram-negative target bacteria. Also provided are novel purified polypeptides, and fragments thereof, and isolated nucleic acids from the bacterium. Further provided are methods of using the bacterium and the purified polypeptides, and fragments thereof, to preserve food and prevent bacterial contamination of food.

The invention relates to a microbial fertilizer. The microbial fertilizer comprises 84-90% by mass of organic matters and 5-11% by mass of water and also comprises a strain, wherein the strain comprises at least two kinds of aerobic bacteria and at least one anaerobic bacterium; each aerobic bacterium is any one of bacillus subtilis, bacillus megaterium, bacillus stearothermophilus, bacillus sphaericus, bacillus firmus and saccharomycetes; the anaerobic bacterium is any one of bacillus circulans, bacillus licheniformis and lactobacillus acidophilus. The microbial fertilizer has the beneficial effects that the microbial fertilizer has the effects of overcoming the defects of the existing chemical fertilizers, reducing environmental pollution and activating soil; the content of beneficial biological bacteria in the microbial fertilizer is more than 0.3billion/g and biological activating enzymes and various trace elements are supplied, so that the quality of crops has a qualitative leap; through simultaneously containing the aerobic bacteria and the anaerobic bacteria, the microbial fertilizer can work under different conditions, thus increasing the output rates of the crops.

The invention discloses a biological filling material and a filling material net containing the same. The invention relates to a biological filling material, which comprises a filling material body. Multiple bacterium clusters are adsorbed on the external wall of the filling material body. Each bacterium cluster is sequentially provided with an anaerobic bacterium layer and an aerobic bacterium layer from the center to the outer wall. Bacterium clusters are adsorbed on the filling material body. Each bacterium cluster comprises an anaerobic bacterium layer and an aerobic bacterium layer. Due to the different growth habits of anaerobic bacterium and aerobic bacterium, the aerobic bacteria are distributed in the external side, the anaerobic bacteria are distributed in the inner side, through the combination of anaerobic bacteria and aerobic bacteria, the number of sewage processing tanks is reduced, and moreover, the processing speed and efficiency are improved.

The invention discloses a compound nitrogen-fixing microorganism bacterium fertilizer and a preparation method thereof, particularly a compound nitrogen-fixing microorganism bacterium fertilizer which is wide in PH application range, strong in nitrogen fixing capacity under aerobic and anaerobic conditions, and easy to store. The compound nitrogen-fixing microorganism bacterium fertilizer is prepared from the following raw materials: 10 to 30 parts of bacillus flexus fermenting liquid, 30 to 80 parts of clostridium pasteurianum fermenting liquid and 20 to 60 parts of India Beijerinckia fermenting liquid. According to the compound nitrogen-fixing microorganism bacterium fertilizer and the preparation method thereof, the aerobic and anaerobic nitrogen fixing bacteria series are fused into a whole creatively; one type is the aerobic nitrogen fixing bacteria, namely, the India Beijerinckia (the India Beijerinckia can fix nitrogen under low oxygen, but is mainly the aerobic bacterium) and the bacillus flexus; the other type is the anaerobic nitrogen fixing bacteria, namely, the clostridium barati; both the aerobic nitrogen fixing bacteria and the anaerobic nitrogen fixing bacteria have strong nitrogen fixing capacity, can fix the nitrogen in relatively deep soil (10 to 30 cm), and can convert nitrogen in air into the nitrogen which can be absorbed by a plant and provide the nitrogen to the plant continuously.

The invention relates to a nano biological cycle water treatment agent which comprises the following raw materials in parts by mass: 10-20 parts of sepiolite fibers, 100-150 parts of pure diatom, 10-20 parts of volcanic carbon, 60-100 parts of attapulgite, 6-10 parts of zeolite, 10-20 parts of an enzymatic agent, 0.02-0.07 part of magnesium hydroxide, 0.02-0.07 part of ferric oxide, 30-50 parts of glucose, 50-80 parts of an aerobic bacterium agent, 20-30 parts of an anaerobic bacterium agent, 10-20 parts of a nitrifying bacterium agent, 10-20 parts of a denitrifying bacterium agent, 10-20 parts of a phosphorus-accumulating bacterium agent and 10-20 parts of a hydrolytic acidification bacterium agent. The preparation method comprises the following steps: sufficiently mixing various materials which are weighed according to a mass ratio, and performing packaging, thereby obtaining a finished product. The water treatment agent can be applied to treatment of various types of hardly-degradable industrial wastewater and has excellent effects of degrading and removing multiple pollutants, and the moisture content of sludge can be reduced.

The invention discloses a root knot nematode disease prevention pseudomonas new species. The strain is a pseudomonas with the collection name of pseudomonas Jdn1 and collected in a China General Microbiological Culture Collection Center (CGMCC) at No. 3, yard 1, Beichen west road, Chaoyang district, Beijing on June 9, 2017, and the collection number of the strain is 14226. The single colony of thepseudomonas is faint yellow, nearly circular, transparent, neat in edge and smooth in surface, the pseudomonas strain is gram-stained to be red and is a Gram negative bacterium, and the pseudomonas strain grows along the surface of a culture medium in an aerobic test result and is an aerobic bacterium. The pseudomonas strain and the microbial agent thereof are good in root knot nematode disease prevention effects and environmentally friendly.

The invention relates to a biological demanganization and deferrization functional bacterium, which relates to a microorganism. The invention solves the problems that: the prior biological oxidation demanganization engineering flora is established in virtue of natural selection, thereby the biochemical capacity is weak and the demanganization effect is bad; the culture, acclimation and starting time required for a water treatment demanganization biological filter chamber is long, and the restore cycle after the enginerring flora is damaged is long. The biological demanganization and deferrization functional bacterium MS604 (Sphingobacterium sp.) belongs to Sphingobacterium sp. and is preserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms; the preservation date is October 30th, 2007 and the preservation No. is CGMCC No.2240; the strain MS604 is a gram negative aerobic bacterium and a polymorphous bacillus with a length of 1 to 2 micrometers and a width of 0.3 to 0.4 micrometers, and both ends of cells are round and obtuse; a milk yellow colony is formed on a culture medium and is round and opaque with tidy edges; the strain MS604 is provided with energy storage particles and also provided with a brood cell and a capsule, and N, N, N', N'-tetramethyl-p-phenylenediamine is dyed to be masculine. The biological demanganization and deferrization functional bacterium MS604 has the capacity for removal of Mn<2+> and Fe<2+> in water; the removal rate of Mn<2+> is higher than 79 percent and the removal rate of Fe<2+> is higher than 90 percent.

Electrodes for electrolysis of water, for encouraging growth of algae and aerobic bacteria, for removing suspended solids from wastewater during treatment, or for rendering water sterile and potable have a concrete coating over a metallic or carbon fibre core. The coating is from 2 to 50 mm thick; preferably 5 to 25 mm thick. Preferably, a DC current passed between the immersed electrodes periodically reversed but there is no visible “rusting” at the anode if the DC current is steady. The resistive nature of the concrete tends to suppress concentrations of current upon the electrode surface.

The invention discloses an aerobic and anaerobic mixture biological agent, and relates to the technical field of microorganisms. The aerobic and anaerobic mixture biological agent comprises 5-15 parts of a carrier, 25-33 parts of a microbial agent and 13-18 parts of a biological compound enzyme preparation, the carrier comprises 1.2-4 parts of bacterial cellulose, 0.8-2 parts of nano activated carbon and 0.5-1 part of kaolinite; the microbial agent comprises aerobic flora and anaerobic flora, and the biological compound enzyme preparation is prepared from 1.7 to 2.5 parts of lipase, 2.5 to 3.3 parts of protease and 0.8 to 1.7 parts of cellulase. The invention further discloses a preparation method of the aerobic and anaerobic mixture biological agent. The preparation method comprises four steps. According to the method, four aerobic bacteria and four anaerobic bacteria are adopted, when the dissolved oxygen content in the environment is low, the anaerobic bacteria are rapidly added into dominant bacteria to rapidly degrade organic pollutants in the environment, and along with gradual increase of the dissolved oxygen content in the environment, the aerobic bacteria are gradually bred into dominant bacteria, so the anaerobic bacteria are replaced to continuously degrade the organic pollutants in the environment; different components work cooperatively, so the overall efficient water body treatment effect is maintained.

The invention provides a method for efficiently producing an organic fertilizer. The method comprises the following steps of: uniformly mixing straw powder, a penetrating agent, ethanol and excrementand then carrying out steam explosion to obtain steam-exploded excrement; adding microbial strains, and introducing oxygen to decompose the steam-exploded excrement; centrifugally separating the obtained decomposed excrement; adjusting the water content of the decomposed excrement by using decomposed filtrate; and mixing and aggregating the solid decomposed excrement with the microbial strains, anutritional agent, auxiliary materials and a binder to obtain the granular organic fertilizer. According to the method provided by the invention, by utilizing steam explosion, the decomposition efficiency of the materials is greatly improved, and the production period of the organic fertilizer is shortened; meanwhile, in the decomposition process, the activity of aerobic bacteria is improved through constant temperature, oxygen introduction and decomposition filtrate spraying; and meanwhile, excrement is loosened by slowly turning over the excrement, and contact between the excrement and oxygen is increased, so that decomposition of the excrement is accelerated, the production period of the organic fertilizer is shortened, and the production efficiency of the organic fertilizer is improved.

The invention provides a method and equipment for drying and fermenting feed at low temperature. The method includes the steps of raw material sterilizing, probiotics inoculating, fermenting, drying, material cooling, smashing and finished product packaging; according to the drying step, the material is dried at constant low temperature of 50 DEG C-90 DEG C, according to the fermenting process, the sterilized raw material is inoculated with an aerobic bacterium solution and then subjected to aerobic fermentation, glucosinolate and acid soluble protein content is monitored online, and when the glucosinolate removal rate and acid soluble protein content start to reduce, the raw material is inoculated with a facultative anaerobe solution to be subjected to facultative anaerobe fermentation. The equipment comprises a heat conducting oil furnace, a low-constant-temperature tube bundle drying drum, a rolling airing drum, a pulverizer, a storage silo and a packager. Low constant temperature drying and staged fermentation are combined and work along both lines, nutritive value and quality uniformity of the feed are guaranteed by increasing the probiotics survival amount and improving the detoxifying effect, and meanwhile production cost is reduced.

The invention belongs to the technical field of bacterium culture and particularly discloses an aerobic bacterium culture method. The aerobic bacterium culture method adopts the following culture system. The culture system comprises a mounting frame, a ventilation type culture dish, a swaying unit and a placement unit; the swaying unit is installed at the upper end of the mounting frame and is used for swaying the ventilation type culture dish; the placement unit is arranged at the middle part of the mounting frame, is fixedly connected with the mounting frame and is used for storing the ventilation type culture dish; and the ventilation type culture dish is arranged on the placement unit and is used for culturing aerobic bacteria. By adopting the aerobic bacterium culture method providedby the invention, through mutual matching of the mounting frame, the swaying unit and the placement unit, back and forth rocking of the ventilation type culture dish in which the aerobic bacteria arelocated is realized, and the problems of overlarge calorific value and too high pH value caused by gathering of too many aerobic bacteria are avoided.

The invention discloses a denitrification system for synchronously achieving nitrification and denitrification and an application method thereof and belongs to the technical field of sewage denitrification treatment. The denitrification system comprises an aerobiotic nitration reaction device, a filtering device and an anaerobic denitrification device. A nitrification reactor is connected with ananaerobic denitrification reactor, single ammonium nitrogen is adopted as a nitrogen source, methane is adopted as a carbon source, nitrification, denitrification, anaerobic methane oxidation and anaerobic ammoxidation are synchronously achieved, and high-efficiency denitrification is achieved. A measure of controlling dissolved oxygen is improved. After 4 hours of feeding water into the nitrification reactor, the dissolved oxygen in the nitrification reactor is reduced to the minimum so as to control the dissolved oxygen in the denitrification part to be 0 mg/L and achieve a strict anaerobicenvironment. A measure for controlling nitrifying bacteria to enter the anaerobic reactor is improved, the filtering device is newly added, the number of aerobic bacteria in the anaerobic denitrification environment is decreased, and the competition of the aerobic bacteria with DAMO microbes and Anammox functional microbes is reduced.

The invention discloses an orange peel pickle and a preparation method thereof. The orange peel pickle comprises, by weight, 1-5 parts of microorganism, 400-800 parts of orange peel, 1-5 parts of inulin, 10-15 parts of glucose, 1-5 parts of polydextrose, 2-8 parts of shitake mushroom extract, 2-5 parts of Sichuan pepper, 20-40 parts of ginger slice, 10-30 parts of garlic, 120-200 parts of salt, 10-20 parts of liquor and 2000-3500 parts of water. According to the method, the orange peel pickle is prepared by fermenting the orange peel with the bacillus lacticus, wherein the bacillus lacticus isan aerobic bacterium which consumes oxygen in the fermentation process to inhibit the mildew of the orange peel and the malignant change of the flavor substances, and has good color protection to effectively prevent browning of the orange peel. The orange peel pickle has the advantages of sweet and sour taste, coordinated taste, good color and good flavor, and has the advantages of invigorating the stomach and helping digestion and achieving nutrition and health care. The orange peel pickle is simple in operation process and suitable for industrial mass production.

The invention discloses a culture waste utilization method based on a fermentation technology, belongs to the field of fermentation, and particularly relates to a culture waste utilization method based on a fermentation technology. The method comprises the following steps: fermenting culture waste to form an organic fertilizer for utilization, and in the process, adding pre-aerated particles intosolid waste after dehydration of the culture waste; during high-temperature composting, water in the hot-melt water ball is in contact with the gas dispersing powder after hot melting of the hot-meltwater ball to generate a large amount of carbon dioxide, on one hand, when the carbon dioxide does not completely overflow to the outside, the concentration of the carbon dioxide in the compost is relatively high, anaerobic bacteria can be effectively stimulated, and the activeness of the anaerobic bacteria is improved; on the other hand, after carbon dioxide completely overflows, the gas containing channel communicated with the outside is formed in the compost, and the outside air can enter the compost so that the survival requirement of aerobic bacteria on oxygen is met, the synchronous action of the aerobic bacteria and anaerobic bacteria can be realized, the time required by composting is remarkably shortened, and the efficiency of preparing the organic fertilizer from the livestock wastes is further improved.