528 results about "Bacterial growth" patented technology

The invention relates to the technical field of microorganisms, in particular to a culture medium and a method for culturing Ackermania muciniphila for activating Amuc-1260 by using lambda-carrageenan as a carbon source, according to the culture method, the lambda-carrageenan is used for replacing a traditional carbon source, thallus growth and proliferation can be remarkably promoted, the effect is optimal under the condition of 2.00 g / L, and the culture cost is low. Compared with the conventional carbon source (glucose), the carbon source is improved by 92.91%. Further research finds that the lambda-carrageenan can induce the Akk bacteria to up-regulate the expression of glycoside hydrolase such as Amuc-1260 and enhance the activity of pathways such as glycosaminoglycan degradation, amino acid metabolism and insulin signals, so that the metabolic adaptability and energy utilization efficiency of the bacteria are improved. Meanwhile, the Akk bacteria secrete more functional short-chain fatty acids such as acetic acid, propionic acid and the like under the condition, and have the effects of regulating intestinal health and maintaining metabolic homeostasis. The invention provides a new theoretical basis and technical support for large-scale culture of Akk bacteria and development of a metabiotic product.

The invention belongs to the technical field of biological detection, and particularly relates to a rapid detection method and a micro-fluidic chip for rapid detection. The existing detection method and instrument have the problems of long detection time, incapability of detecting unknown pathogenic bacteria and the like, and cannot meet the timeliness requirement of clinical application. According to the scheme, rapid detection of the drug resistance of bacteria is realized by detecting the growth state of high-throughput single bacteria, and the method comprises the following steps: culturing the bacteria by using the micro-fluidic chip designed in the invention, capturing the single bacteria through a single bacteria capturing channel, and continuously growing in the channel; and obtaining a morphological change matrix of single bacteria evolved along with time in each bacteria capture channel, carrying out statistical analysis to obtain a bacteria phenotype index, and further obtaining the drug tolerance condition of the bacteria on the basis of index numerical value change. According to the method, the function of rapidly detecting the drug resistance of various common clinical pathogenic bacteria within 2 hours can be realized.

The invention provides an integrated disinfection monitoring management system based on quantitative risk assessment, and belongs to the technical field of disinfection monitoring. Comprising an environment and behavior parameter acquisition module, a bacteria breeding risk analysis module and a disinfection quality analysis module, the environment and behavior parameter acquisition module is used for constructing a first data set, a second data set and a third data set, and constructing a prediction risk model by using a convolutional neural network; and based on the first data set, the second data set and the third data set, respectively constructing an environment bacterium breeding coefficient, a bacterium propagation coefficient and a behavior bacterium breeding coefficient of the ith area, combining the environment bacterium breeding coefficient and the behavior bacterium breeding coefficient of the ith area to construct a comprehensive disinfection behavior coefficient, and performing evaluation to generate a corresponding strategy. In-station bacteria monitoring is achieved through multi-dimensional linkage, and a proper disinfection scheme is adopted according to the bacterium breeding degree.

The invention discloses a quick-cooling ice-making table water purifying and drinking machine. A water refrigerating device comprises a cold container water inlet pump, a cold container, a compressor, a drying filter, a condenser, a cold container evaporator, a reversing electromagnetic valve and a cold container water outlet pump. The ice making device comprises an ice making upper shell, an ice mold evaporator, a compressor, an ice mold evaporator, a deicing electromagnetic valve, a funnel assembly and an ice block conveying assembly, two evaporators, namely a cold container evaporator and the ice mold evaporator are arranged, and pure water in a cold container is refrigerated to 1-2 DEG C through cooperation of the cold container evaporator; when ice blocks are needed, the funnel assembly in the cold container moves upwards, full cold water is conveyed to the ice making module, the ice making module is matched with the ice mold evaporator to make the ice blocks, the funnel assembly moves downwards, the ice block conveying assembly moves to the position below the ice making module, the ice blocks are conveyed out of the ice outlet after ice melting, the whole ice making process is completed within 50 seconds, and quick ice making through cooling and quick ice block making are achieved. The ice blocks are used when being made, and the problems that the ice blocks breed bacteria, and no ice is stored and condensed are effectively solved.

The invention discloses a construction method of a nocardia seriolae mycolic acid deficient strain, which belongs to the technical field of aquaculture prevention and control, and comprises the following steps: strain and material preparation, culture and genome extraction, upstream and downstream fragment cloning to construct deletion plasmids, competent cell preparation, electrotransformation and screening, and biological characteristic and toxicity analysis. The nocardia seriola seriola mycolic acid defect type strain is constructed and compared with a wild strain and a back-filling strain for research, the effect of the mycolic acid in the aspects of growth, form, toxicity and the like of thalli can be defined, the relationship between the mycolic acid and the pathogenicity of the thalli can be deeply known, and an important clue is provided for revealing the pathogenic mechanism of the nocardia seriola seriola.

The application belongs to the field of medicine, and particularly relates to a cell penetrating peptide Phage-Dec and application thereof in antibiosis. The cell penetrating peptide Phage-Dec has excellent cell penetration ability and antibacterial activity, can effectively penetrate the cell membrane, target and inhibit bacterial growth, and thus achieves the purpose of antibiosis. Research shows that Phage-Dec has a significant inhibitory effect on various pathogenic bacteria, and can effectively reduce the reproduction speed of bacteria. The use of the cell penetrating peptide provides a new idea for antibacterial treatment, has a good clinical application prospect, and lays a solid foundation for developing a new antibacterial drug based on a bacteriophage source.

The invention discloses a preparation method of a bacteriostatic bamboo fiber composite material, and relates to the technical field of bacteriostatic bamboo fiber composites.The preparation method comprises the following steps that firstly, bamboo fibers are subjected to biological enzyme degumming treatment and dried till the water content is smaller than or equal to 5%; 2, a periodic nanorod array is formed on the surface of the bamboo fiber through a laser-induced self-assembly technology, the laser wavelength is 266-1064 nm, the pulse frequency is 10-50 kHz, the height of nanorods is 50-200 nm, and the density is 10-30 nanorods per mu m < 2 >. Through laser wavelength and pulse frequency, the height and density of the nano-column are accurately regulated and controlled, the nano-column pierces a bacterial cell membrane, the overall bacteriostasis rate is improved, the stress dispersion and tensile strength of the nano-column are obviously improved, the nano-column structure is combined with fluorination modification, the hydrophobic contact angle is better, and bacterial breeding caused by liquid retention is reduced.

The invention belongs to the technical field of medical instruments, and particularly relates to wound cleaning and nursing equipment for trauma, which comprises a base, a lifting and positioning mechanism, a support frame and a flushing and blowing mechanism are arranged on the base, a sliding groove is formed in the top of the support frame, and a sliding block is slidably arranged in the sliding groove; the flushing and blowing mechanism comprises a driving assembly, a liquid storage tank and a gas storage tank, and the driving assembly is in transmission connection with the liquid storage tank and the gas storage tank; cooperative operation of stable conveying of flushing fluid and rapid blowing-out of gas can be achieved, wounds and peripheral skin can be dried through the blowing head in time after cleaning, wiping injury is avoided, the risk of bacterium breeding is reduced, time consumed by the nursing process is shortened, extrusion pressure of the flushing fluid in the fluid storage tank can be controlled, and the nursing efficiency is improved. And the problem that the wound is secondarily damaged due to excessive pressure or the cleaning is not thorough due to excessive pressure is effectively avoided.

The invention provides an antibacterial peptide and application thereof in resisting bacteria and / or enhancing the disease resistance of plants. Specifically, on one hand, the invention provides two antibacterial peptides which are novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4, sequences of the novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4 are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the antibacterial peptides ZJUAMP3 and ZJUAMP4 both have broad-spectrum antibacterial activity, have good antibacterial activity on bacteria such as gram-negative bacteria, gram-positive bacteria and fungi, and are suitable for scenes needing sterilization or fungus and bacterium growth inhibition. Besides, the ZJUAMP4 can activate an MAPK signal channel in a plant body and induce the plant to generate systematic disease resistance, and is suitable for the fields of crop disease prevention and control and plant protection. The antibacterial peptide is simple in structure and easy to synthesize.

The present invention provides use of a C-JUN N-terminal kinase inhibitor, SU3327, in the preparation of a medicament for enhancing the efficacy of polymyxin against bacterial infection, and use of a composition of SU3327 and polymyxin in the preparation of a medicament for an enhanced efficacy against bacterial infection, wherein the polymyxin is preferably Polymyxin E. The invention not only shows an enhanced antibacterial activity of polymyxin by SU3327 in synergy through minimum inhibitory concentration tests in checkerboard assays and in vitro bacterial growth curves, but also confirms the ability of SU3327 to enhance polymyxin along with its effectiveness in vivo in the experiments with a model of mice infected with drug resistant bacteria at the animal level. The invention provides a new use of SU3327 in enhancing antibacterial activities of polymyxin antibiotics, resolving the technical problems such as clinical resistance to polymyxin, low clinical therapeutic index of polymyxin, etc.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

The invention belongs to the technical field of colloid filling, and particularly relates to recombinant collagen gel subpackaging equipment which comprises a bearing part, a material injection mechanism, a preheating mechanism and a recycling mechanism. The bearing part comprises a bearing disc, a supporting frame and a bearing plate, the bearing disc is fixedly mounted on the outer wall of the supporting frame, and the bearing plate is slidably arranged on the outer wall of the supporting frame; the material injection mechanism comprises a buffer box, an adapter block and a material injection pipe, the buffer box is fixedly mounted on the upper end face of the bearing plate, and the material injection pipe communicates with an inner cavity of the buffer box through the adapter block; the preheating mechanism comprises a storage box and a wrapping pipe, the storage box is fixedly installed on the side wall of the bearing plate, a heating piece is arranged in the storage box, and the wrapping pipe is arranged outside the material injection pipe; the recycling mechanism comprises a backflow box and a condensation plate; the condensation plate is fixedly mounted on the inner wall of the backflow box; through cooperation of the structures, the filling process is smoother, the filling efficiency is improved, material residues on the inner wall of the material injection pipe are reduced, and the risk of bacterium breeding after shutdown is reduced.

Provided herein are kits, compositions, systems, and methods for treating a subject with a tumor with kynurenine responsive bacteria (e.g., such that the bacteria reduce or eliminate said tumor). In certain embodiments, the kynurenine responsive bacteria comprise one or more nucleic acid sequences comprising: i) a first nucleotide sequence (e.g., not endogenous to said bacteria) encoding a kynurenine transcriptional regulator protein, and ii) a second nucleotide sequence (e.g., not endogenous to said bacteria or overexpressing an endogenous equivalent protein) encoding a kynurenine transporter protein. In particular embodiments, the kynurenine responsive bacteria further comprise at least one of the following: i) a third nucleotide sequence comprising a kynurenine responsive promoter upstream of a plasmid replication gene encoding a plasmid replication protein, ii) a fourth nucleotide sequence comprising a kynurenine responsive promoter upstream of a reporter gene encoding a reporter protein, and iii) a fifth or more nucleotide sequence comprising a kynurenine responsive promoter upstream of a gene essential for bacterial growth, or a gene codes a for therapeutic payload.

The invention provides periorbital secretion inhibition gel containing natural plant polyphenols and a preparation method thereof, and relates to the field of pet eye care, the gel is chelated with iron ions through EGCG to block a porphyrin oxidation pathway, cooperates with zinc ions to inhibit bacterial growth and plays a role in multiple mechanisms of targeted removal of bacterial biofilms through antibacterial peptide LL-37; in-vitro tests prove that the functional components in the gel have a remarkable synergistic effect, proliferation of staphylococcus aureus can be effectively inhibited, deposition of porphyrin is remarkably inhibited, and formation of tear stains is relieved; animal experiment results show that the gel has a remarkable tear stain removal effect and good periorbital safety, after two weeks of treatment, the periorbital tear stain area is reduced by 75.97% compared with that of a bear dog, the eye McDonald-Shiadduck stimulation score is also remarkably reduced, corneal fluorescein staining detection results of all tested animals are negative, and the gel has a good clinical application prospect. A mild and effective new thought is provided for inhibiting the periocular secretions of the pets.

The invention provides refrigeration equipment which comprises an ice-making chamber and an ice maker installed in the ice-making chamber, an ice-making refrigeration module is installed in the ice-making chamber and used for supplying cold air to the ice-making chamber, and the ice-making refrigeration module comprises a refrigeration shell installed in the ice-making chamber. The refrigeration shell comprises a first shell body and a second shell body connected with the first shell body, and the first shell body and the second shell body are connected to form an evaporator chamber in a surrounding mode. An ice-making evaporator, a defrosting heating wire and a water pan are installed in the evaporator chamber. The ice-making refrigeration module can be used as an independent module to be mounted at a supplier, so that the mounting precision of the ice-making refrigeration module is ensured, the field mounting difficulty of the refrigeration equipment is reduced, and the field mounting time of the refrigeration equipment is shortened. In addition, the ice making machine in the ice making chamber is cooled by the independent ice making refrigeration module, so that the problem that the ice cubes made by the ice making machine are mixed in flavor or microorganisms in the storage chamber flow into the ice making chamber to cause bacterium breeding is solved.

The invention belongs to the technical field of medicines, and particularly relates to application of 10-undecylenic acid and polymyxin in preparation of an antibacterial drug, and the antibacterial drug aims at one or more of escherichia coli, salmonella and klebsiella pneumoniae. The antibacterial agent aims at bacteria which are resistant to polymyxin. The bacteria directed by the antibacterial agent are multi-drug-resistant gram-negative bacteria. Through a chessboard method minimum inhibitory concentration test, an in-vitro bacterial growth curve proves that 10-undecylenic acid synergistically enhances the antibacterial activity of polymyxin, and meanwhile, a mouse drug-resistant bacterial infection model test proves that 10-undecylenic acid can effectively enhance the in-vivo effectiveness of polymyxin at the animal level. The invention provides a new application of 10-undecylenic acid in enhancing the antibacterial activity of polymyxin antibiotics, and can solve the technical problems of low clinical drug resistance and therapeutic index of polymyxin and the like.

Provided a method for promoting growth of bacteria of the genus Bifidobacterium. The method for promoting growth of bacteria of the genus Bifidobacterium includes subjecting a milk culture medium containing one or more selected from the group consisting of cysteine, cystine, and a salt thereof to heat treatment and then culturing bacteria of the genus Bifidobacterium in the milk culture medium.

The invention discloses a breeding device suitable for reptiles and amphibians, and relates to the technical field of animal breeding, the breeding device comprises a base plate, a cleaning mechanism is arranged in the base plate, the cleaning mechanism comprises a driving motor arranged in the base plate, and the output end of the driving motor is provided with a threaded rod; a movable plate is in threaded connection with the side, close to the driving motor, of the threaded rod, a cleaning soft plate is fixedly connected below the movable plate, a feeding trough is fixedly connected below the base plate, a telescopic water pipe is fixedly connected with the side, close to the cleaning soft plate, of the movable plate, and a spray head is arranged on the side, away from the movable plate, of the telescopic water pipe. A water pump box is arranged on the side, away from the spray head, of the telescopic water pipe, a driving motor is started to drive a moving plate to move through a threaded rod, the moving plate drives a cleaning soft plate to slide on the inner wall of the feeding trough when moving, comprehensive and dead-corner-free cleaning of the inner wall of the feeding trough is achieved, and through the design, feed residues at corners of the device are effectively avoided; the possibility of bacterium breeding is reduced, and the health of animals is guaranteed.

The invention provides a method for improving fermentation yield and sugar-acid conversion rate of 5-hydroxytryptophan, alpha-ketoglutaric acid ferrous salt, alpha-ketoglutaric acid zinc salt, alpha-ketoglutaric acid cobalt salt and alpha-ketoglutaric acid manganese salt are added into a fermentation culture medium according to a specific proportion, alpha-ketoglutaric acid directly participates in TCA circulation, circulation operation can be accelerated, generation of NADH and FADH2 is increased, the yield of 5-hydroxytryptophan is increased, and the yield of 5-hydroxytryptophan is increased. The ATP synthesis efficiency is improved, and more energy is provided for cell growth; the alpha-ketoglutaric acid can balance amino acids required in the growth process of escherichia coli, promote the generation of proteins and nucleic acids and accelerate the growth of thalli; the alpha-ketoglutaric acid metal salt can improve the utilization efficiency of microelements by thalli; ferrous ions are cofactors of 5-hydroxytryptophan hydroxylase and can accelerate the hydroxylation process of tryptophan. Through the synergistic effect of the added components, the yield of the 5-hydroxytryptophan and the sugar-acid conversion rate are improved.

The invention relates to the field of medical instruments, in particular to an electric artery nasal irrigator convenient to disassemble and replace. According to the electric artery nasal irrigator convenient to disassemble and replace, the overall structure can be conveniently disassembled and replaced, bacterium breeding is prevented, and the service life of the electric artery nasal irrigator convenient to disassemble and replace is prolonged. An electric artery nasal irrigator convenient to disassemble and replace comprises a water storage bottle, a first shell, a second shell and the like, the left portion of the water storage bottle is in threaded connection with the first shell, and the right portion of the water storage bottle is in threaded connection with the second shell. A second limiting frame is rotated, then a nozzle is moved upwards to be taken out, then a water outlet pipe is rotated, so that a connecting thread sleeve moves upwards to be separated from a first shell and a second shell, then the first shell and the second shell are rotated, the first shell and the second shell are separated from a water storage bottle, and the overall structure can be conveniently disassembled and replaced; bacteria breeding is prevented, and the service life of equipment is prolonged.

The invention relates to the field of animal experiments, and discloses an animal experiment system based on concealed transportation and self-purification and a management method.The animal experiment system comprises a feeding module and a control module, the feeding module comprises a plurality of independent cages, and temperature and humidity sensors, miniature air supply and exhaust modules and cameras are arranged in the independent cages; the transportation module comprises a concealed rail, and the concealed rail is used for connecting the feeding module and the operation module; the operation module comprises a lifting type operation table, the lifting type operation table is provided with a mechanical arm and an isolation barrier, and the operation module further comprises a health monitoring station. Low-noise and stable hidden transportation is achieved through the magnetic suspension driving technology of a hidden track and a sound insulation shielding layer covering the outside, the bottom of each independent cage is provided with a microbial degradation layer composed of an immobilized bacillus subtilis film and a porous ceramic substrate, pollutants can be continuously and efficiently decomposed, peculiar smells and bacterium breeding are reduced, and the environment is protected. Therefore, experimental data can truly and accurately reflect the influence of experimental variables.

An apparatus (1) comprising an optical device (2) for monitoring bacterial growth of a dosed liquid biological sample. The apparatus is provided with a sample container port for receiving, in use, a sample container (6) having at least one detection chamber (20) for containing a dosed sample. The optical device (2) comprises a light source (22) configured to emit light along an incident beam axis which, in use, intersects at least one detection chamber (20) of the sample container (6) and illuminates a dosed sample contained in the detection chamber (20). The optical device (20) comprises a first photodetector (26) configured to receive light scattered by bacteria in the sample. The optical device (2) comprises a light collection arrangement (24) configured to collect light exiting the detection chamber (20) that has been positively scattered by bacteria in the sample at a scattering angle range of between about + / - 4 and + / - 20 degrees relative to the incident beam axis and direct the collected scattered light to the first photodetector (26); and prevent non-scattered light travelling parallel to the incident beam axis and exiting the detection chamber (20) from reaching the first photodetector (26). The optical device (2) comprises at least one processor configured to: measure an intensity of the scattered light received by the first photodetector (26); determine a respective representative quantity or concentration of bacteria in the sample from the intensity of the scattered light; repeat the measuring and determining steps at a series of predetermined intervals to determine changes in the representative quantity or concentration of bacteria in the sample over time; and determine a respective susceptibility of the bacteria in the sample to a respective drug.

The invention provides an observation device of a bacterial incubator, and relates to the field of bacterial culture. A box door is rotationally connected to a box opening of the bacterial incubator main body, and three rectangular through holes are uniformly formed in the front end surface of the box door; three supporting shelves are uniformly mounted in the bacterial incubator main body; the device is characterized in that the front end of the box door is located at the front sides of the three rectangular ports and is rotationally connected with three transparent observation plates; through cooperation of the treading type driving assembly and the fog scraping assembly, when fog generated on the rear end face of the transparent observation plate needs to be scraped off, the box door does not need to be opened, the situation that the box door is frequently opened is avoided, and therefore normal growth of bacteria is prevented from being affected; the problems that in the using process of an observation window on an existing bacterial incubator door, when mist on the inner side face of the observation window needs to be scraped off, the whole incubator door needs to be opened, and normal growth of bacteria is affected when the incubator door is frequently opened are solved.

The present invention pertains to use of sodium diacetate (NaHAC2) as an antimicrobial agent against bacteria growing in biofilms. The aspects of the invention include a wound care product comprising sodium diacetate, a kit comprising a wound care product, and a method of treating an infected wound.

The application discloses an antibacterial peptide RV15 and application thereof, and belongs to the technical field of biology. The amino acid sequence of the antibacterial peptide RV15 is shown as SEQ ID NO. 1. The antibacterial peptide RV15 provided by the application has broad-spectrum antibacterial activity, has a significant bacteriostatic effect on gram-positive bacteria and gram-negative bacteria, and has a long bactericidal duration. The hemolytic activity is very low, and the biological safety is good. The antibacterial peptide RV15 can be used for treating bacterial infection, and can also be applied to other scenes requiring bactericidal action or bacterial growth inhibition. The antibacterial peptide RV15 provided by the application has a short sequence, a small molecular weight and low chemical synthesis difficulty, and can save the production cost on a large scale.

The present invention discloses a damping cloth for final polishing of optical elements and a preparation process thereof, belonging to the technical field of damping cloth. A damping cloth for final polishing of optical elements comprises an elastic base layer, a water-repellent layer and an abrasive layer in sequence from the polishing contact surface to the adsorption surface. The water-repellent layer comprises a water-isolating layer and a buffer layer. The buffer layer is made of a polyurethane foam material containing modified glass powder, and the water-isolating layer is a polyurethane waterproof film. The present invention solves the problem that the surface of the existing damping cloth is prone to bacterial growth and mildew. The present invention proposes a damping cloth for final polishing of optical elements and a preparation process thereof. The elastic base layer adopts a structure in which an outer layer covers a fabric layer, thereby improving the flatness of the entire damping pad. The wood ash component in the modified glass powder can increase the alkalinity of the polishing liquid. The wood ash itself has a bactericidal effect, which can prevent the damping cloth from breeding bacteria and mildew, thereby improving the service life of the damping cloth.

Provided is a composition for inhibiting the proliferation of bacteria belonging to the genus Collinsella, the composition containing at least one selected from sugar acid-containing oligosaccharides and salts thereof. The sugar acid is preferably selected from aldonic acid, uronic acid, and aldaric acid.

The present invention belongs to the field of molecular biology technology, and in particular relates to a recombinant protein of the CgVg functional domain DUF1943 and VWD of the long oyster, as well as a preparation method and application thereof. The amino acid sequence of the recombinant protein of the CgVg functional domain DUF1943 of the long oyster is shown in SEQ ID NO.1. The amino acid sequence of the recombinant protein of the CgVg functional domain VWD of the long oyster is shown in SEQ ID NO.2. The recombinant proteins of the CgVg functional domain DUF1943 and VWD of the long oyster obtained by the present invention have a wide range of binding activity against microorganisms, among which the DUF1943 recombinant protein has the activity of agglutinating bacteria and fungi, and the VWD recombinant protein has a broad spectrum of activity in inhibiting bacterial growth, and can be applied to the research and development of antibacterial drugs, immunopotentiators and feed additives for marine organisms.

The invention discloses an intelligent distribution scheduling method and device for egg products and a medium, and relates to the technical field of intelligent scheduling, and the method comprises the steps: collecting biological characteristic parameters of target egg products in real time, carrying out the feature encryption processing through an edge calculation node to form private data packets, receiving the private data packets of all suppliers through a message queue service, and sending the private data packets to a server; calling a homomorphic encryption library to execute weighted average aggregation on the encrypted feature vectors; and loading real-time road condition data, compartment temperature and humidity distribution and a vibration spectrum in a digital twin sandbox based on a global egg rheology knowledge graph and a microbial growth kinetic model, and coupling to solve a product risk value of a protein shear stress accumulation equation and a flora growth equation. By constructing a global egg product rheology knowledge graph and a microbial growth kinetic model, the time-space coupling characteristics of biomechanical characteristics and microbial activity of egg products in transportation are accurately captured, and the quality risk quantification precision and the transportation strategy reliability are improved.

The invention discloses a medicine for inhibiting helicobacter pylori infection, the medicine is oridonin, and the structural formula of the medicine is as shown in formula 1. In-vitro experiments show that the oridonin has a remarkable antibacterial effect on helicobacter pylori strains SS1, ATCC700392 and ICDC11101, and can remarkably reduce the urease activity of the helicobacter pylori, inhibit the formation of a biological membrane, destroy the growth of thalli and reduce the adhesion effect of the helicobacter pylori on gastric epithelial cells, and in addition, the oridonin and antibiotics are combined for use, so that the effect of preventing and treating the gastric epithelial cells can be achieved. The compound has a synergistic antibacterial effect with metronidazole and clarithromycin, has the effect of reducing the dosage of antibiotics in clinical application, and has potential application value for improving the current drug resistance situation of the antibiotics.