358 results about "Bacterial growth" patented technology

The invention relates to the technical field of microorganisms, in particular to a culture medium and a method for culturing Ackermania muciniphila for activating Amuc-1260 by using lambda-carrageenan as a carbon source, according to the culture method, the lambda-carrageenan is used for replacing a traditional carbon source, thallus growth and proliferation can be remarkably promoted, the effect is optimal under the condition of 2.00 g / L, and the culture cost is low. Compared with the conventional carbon source (glucose), the carbon source is improved by 92.91%. Further research finds that the lambda-carrageenan can induce the Akk bacteria to up-regulate the expression of glycoside hydrolase such as Amuc-1260 and enhance the activity of pathways such as glycosaminoglycan degradation, amino acid metabolism and insulin signals, so that the metabolic adaptability and energy utilization efficiency of the bacteria are improved. Meanwhile, the Akk bacteria secrete more functional short-chain fatty acids such as acetic acid, propionic acid and the like under the condition, and have the effects of regulating intestinal health and maintaining metabolic homeostasis. The invention provides a new theoretical basis and technical support for large-scale culture of Akk bacteria and development of a metabiotic product.

The invention belongs to the technical field of biological detection, and particularly relates to a rapid detection method and a micro-fluidic chip for rapid detection. The existing detection method and instrument have the problems of long detection time, incapability of detecting unknown pathogenic bacteria and the like, and cannot meet the timeliness requirement of clinical application. According to the scheme, rapid detection of the drug resistance of bacteria is realized by detecting the growth state of high-throughput single bacteria, and the method comprises the following steps: culturing the bacteria by using the micro-fluidic chip designed in the invention, capturing the single bacteria through a single bacteria capturing channel, and continuously growing in the channel; and obtaining a morphological change matrix of single bacteria evolved along with time in each bacteria capture channel, carrying out statistical analysis to obtain a bacteria phenotype index, and further obtaining the drug tolerance condition of the bacteria on the basis of index numerical value change. According to the method, the function of rapidly detecting the drug resistance of various common clinical pathogenic bacteria within 2 hours can be realized.

The invention discloses a quick-cooling ice-making table water purifying and drinking machine. A water refrigerating device comprises a cold container water inlet pump, a cold container, a compressor, a drying filter, a condenser, a cold container evaporator, a reversing electromagnetic valve and a cold container water outlet pump. The ice making device comprises an ice making upper shell, an ice mold evaporator, a compressor, an ice mold evaporator, a deicing electromagnetic valve, a funnel assembly and an ice block conveying assembly, two evaporators, namely a cold container evaporator and the ice mold evaporator are arranged, and pure water in a cold container is refrigerated to 1-2 DEG C through cooperation of the cold container evaporator; when ice blocks are needed, the funnel assembly in the cold container moves upwards, full cold water is conveyed to the ice making module, the ice making module is matched with the ice mold evaporator to make the ice blocks, the funnel assembly moves downwards, the ice block conveying assembly moves to the position below the ice making module, the ice blocks are conveyed out of the ice outlet after ice melting, the whole ice making process is completed within 50 seconds, and quick ice making through cooling and quick ice block making are achieved. The ice blocks are used when being made, and the problems that the ice blocks breed bacteria, and no ice is stored and condensed are effectively solved.

The application belongs to the field of medicine, and particularly relates to a cell penetrating peptide Phage-Dec and application thereof in antibiosis. The cell penetrating peptide Phage-Dec has excellent cell penetration ability and antibacterial activity, can effectively penetrate the cell membrane, target and inhibit bacterial growth, and thus achieves the purpose of antibiosis. Research shows that Phage-Dec has a significant inhibitory effect on various pathogenic bacteria, and can effectively reduce the reproduction speed of bacteria. The use of the cell penetrating peptide provides a new idea for antibacterial treatment, has a good clinical application prospect, and lays a solid foundation for developing a new antibacterial drug based on a bacteriophage source.

The invention belongs to the technical field of medical instruments, and particularly relates to wound cleaning and nursing equipment for trauma, which comprises a base, a lifting and positioning mechanism, a support frame and a flushing and blowing mechanism are arranged on the base, a sliding groove is formed in the top of the support frame, and a sliding block is slidably arranged in the sliding groove; the flushing and blowing mechanism comprises a driving assembly, a liquid storage tank and a gas storage tank, and the driving assembly is in transmission connection with the liquid storage tank and the gas storage tank; cooperative operation of stable conveying of flushing fluid and rapid blowing-out of gas can be achieved, wounds and peripheral skin can be dried through the blowing head in time after cleaning, wiping injury is avoided, the risk of bacterium breeding is reduced, time consumed by the nursing process is shortened, extrusion pressure of the flushing fluid in the fluid storage tank can be controlled, and the nursing efficiency is improved. And the problem that the wound is secondarily damaged due to excessive pressure or the cleaning is not thorough due to excessive pressure is effectively avoided.

The invention provides an antibacterial peptide and application thereof in resisting bacteria and / or enhancing the disease resistance of plants. Specifically, on one hand, the invention provides two antibacterial peptides which are novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4, sequences of the novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4 are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the antibacterial peptides ZJUAMP3 and ZJUAMP4 both have broad-spectrum antibacterial activity, have good antibacterial activity on bacteria such as gram-negative bacteria, gram-positive bacteria and fungi, and are suitable for scenes needing sterilization or fungus and bacterium growth inhibition. Besides, the ZJUAMP4 can activate an MAPK signal channel in a plant body and induce the plant to generate systematic disease resistance, and is suitable for the fields of crop disease prevention and control and plant protection. The antibacterial peptide is simple in structure and easy to synthesize.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

Provided a method for promoting growth of bacteria of the genus Bifidobacterium. The method for promoting growth of bacteria of the genus Bifidobacterium includes subjecting a milk culture medium containing one or more selected from the group consisting of cysteine, cystine, and a salt thereof to heat treatment and then culturing bacteria of the genus Bifidobacterium in the milk culture medium.

The invention relates to the field of animal experiments, and discloses an animal experiment system based on concealed transportation and self-purification and a management method.The animal experiment system comprises a feeding module and a control module, the feeding module comprises a plurality of independent cages, and temperature and humidity sensors, miniature air supply and exhaust modules and cameras are arranged in the independent cages; the transportation module comprises a concealed rail, and the concealed rail is used for connecting the feeding module and the operation module; the operation module comprises a lifting type operation table, the lifting type operation table is provided with a mechanical arm and an isolation barrier, and the operation module further comprises a health monitoring station. Low-noise and stable hidden transportation is achieved through the magnetic suspension driving technology of a hidden track and a sound insulation shielding layer covering the outside, the bottom of each independent cage is provided with a microbial degradation layer composed of an immobilized bacillus subtilis film and a porous ceramic substrate, pollutants can be continuously and efficiently decomposed, peculiar smells and bacterium breeding are reduced, and the environment is protected. Therefore, experimental data can truly and accurately reflect the influence of experimental variables.

An apparatus (1) comprising an optical device (2) for monitoring bacterial growth of a dosed liquid biological sample. The apparatus is provided with a sample container port for receiving, in use, a sample container (6) having at least one detection chamber (20) for containing a dosed sample. The optical device (2) comprises a light source (22) configured to emit light along an incident beam axis which, in use, intersects at least one detection chamber (20) of the sample container (6) and illuminates a dosed sample contained in the detection chamber (20). The optical device (20) comprises a first photodetector (26) configured to receive light scattered by bacteria in the sample. The optical device (2) comprises a light collection arrangement (24) configured to collect light exiting the detection chamber (20) that has been positively scattered by bacteria in the sample at a scattering angle range of between about + / - 4 and + / - 20 degrees relative to the incident beam axis and direct the collected scattered light to the first photodetector (26); and prevent non-scattered light travelling parallel to the incident beam axis and exiting the detection chamber (20) from reaching the first photodetector (26). The optical device (2) comprises at least one processor configured to: measure an intensity of the scattered light received by the first photodetector (26); determine a respective representative quantity or concentration of bacteria in the sample from the intensity of the scattered light; repeat the measuring and determining steps at a series of predetermined intervals to determine changes in the representative quantity or concentration of bacteria in the sample over time; and determine a respective susceptibility of the bacteria in the sample to a respective drug.

The invention provides an observation device of a bacterial incubator, and relates to the field of bacterial culture. A box door is rotationally connected to a box opening of the bacterial incubator main body, and three rectangular through holes are uniformly formed in the front end surface of the box door; three supporting shelves are uniformly mounted in the bacterial incubator main body; the device is characterized in that the front end of the box door is located at the front sides of the three rectangular ports and is rotationally connected with three transparent observation plates; through cooperation of the treading type driving assembly and the fog scraping assembly, when fog generated on the rear end face of the transparent observation plate needs to be scraped off, the box door does not need to be opened, the situation that the box door is frequently opened is avoided, and therefore normal growth of bacteria is prevented from being affected; the problems that in the using process of an observation window on an existing bacterial incubator door, when mist on the inner side face of the observation window needs to be scraped off, the whole incubator door needs to be opened, and normal growth of bacteria is affected when the incubator door is frequently opened are solved.

The present invention pertains to use of sodium diacetate (NaHAC2) as an antimicrobial agent against bacteria growing in biofilms. The aspects of the invention include a wound care product comprising sodium diacetate, a kit comprising a wound care product, and a method of treating an infected wound.

The application discloses an antibacterial peptide RV15 and application thereof, and belongs to the technical field of biology. The amino acid sequence of the antibacterial peptide RV15 is shown as SEQ ID NO. 1. The antibacterial peptide RV15 provided by the application has broad-spectrum antibacterial activity, has a significant bacteriostatic effect on gram-positive bacteria and gram-negative bacteria, and has a long bactericidal duration. The hemolytic activity is very low, and the biological safety is good. The antibacterial peptide RV15 can be used for treating bacterial infection, and can also be applied to other scenes requiring bactericidal action or bacterial growth inhibition. The antibacterial peptide RV15 provided by the application has a short sequence, a small molecular weight and low chemical synthesis difficulty, and can save the production cost on a large scale.

Provided is a composition for inhibiting the proliferation of bacteria belonging to the genus Collinsella, the composition containing at least one selected from sugar acid-containing oligosaccharides and salts thereof. The sugar acid is preferably selected from aldonic acid, uronic acid, and aldaric acid.

The present invention belongs to the field of molecular biology technology, and in particular relates to a recombinant protein of the CgVg functional domain DUF1943 and VWD of the long oyster, as well as a preparation method and application thereof. The amino acid sequence of the recombinant protein of the CgVg functional domain DUF1943 of the long oyster is shown in SEQ ID NO.1. The amino acid sequence of the recombinant protein of the CgVg functional domain VWD of the long oyster is shown in SEQ ID NO.2. The recombinant proteins of the CgVg functional domain DUF1943 and VWD of the long oyster obtained by the present invention have a wide range of binding activity against microorganisms, among which the DUF1943 recombinant protein has the activity of agglutinating bacteria and fungi, and the VWD recombinant protein has a broad spectrum of activity in inhibiting bacterial growth, and can be applied to the research and development of antibacterial drugs, immunopotentiators and feed additives for marine organisms.

The invention discloses an intelligent distribution scheduling method and device for egg products and a medium, and relates to the technical field of intelligent scheduling, and the method comprises the steps: collecting biological characteristic parameters of target egg products in real time, carrying out the feature encryption processing through an edge calculation node to form private data packets, receiving the private data packets of all suppliers through a message queue service, and sending the private data packets to a server; calling a homomorphic encryption library to execute weighted average aggregation on the encrypted feature vectors; and loading real-time road condition data, compartment temperature and humidity distribution and a vibration spectrum in a digital twin sandbox based on a global egg rheology knowledge graph and a microbial growth kinetic model, and coupling to solve a product risk value of a protein shear stress accumulation equation and a flora growth equation. By constructing a global egg product rheology knowledge graph and a microbial growth kinetic model, the time-space coupling characteristics of biomechanical characteristics and microbial activity of egg products in transportation are accurately captured, and the quality risk quantification precision and the transportation strategy reliability are improved.

The invention discloses a medicine for inhibiting helicobacter pylori infection, the medicine is oridonin, and the structural formula of the medicine is as shown in formula 1. In-vitro experiments show that the oridonin has a remarkable antibacterial effect on helicobacter pylori strains SS1, ATCC700392 and ICDC11101, and can remarkably reduce the urease activity of the helicobacter pylori, inhibit the formation of a biological membrane, destroy the growth of thalli and reduce the adhesion effect of the helicobacter pylori on gastric epithelial cells, and in addition, the oridonin and antibiotics are combined for use, so that the effect of preventing and treating the gastric epithelial cells can be achieved. The compound has a synergistic antibacterial effect with metronidazole and clarithromycin, has the effect of reducing the dosage of antibiotics in clinical application, and has potential application value for improving the current drug resistance situation of the antibiotics.

The invention provides a sewage tank component, cleaning equipment and a control method of the cleaning equipment. The cleaning equipment comprises a sewage tank, an infusion pump and a control module. The sewage tank comprises a first cavity and a second cavity, the first cavity is provided with a sewage inlet, and a first water level detection device is arranged in the first cavity; the infusion pump is arranged in the sewage tank and is used for pumping sewage in the first cavity to the second cavity; the control module is electrically connected with the first water level detection device and the infusion pump, the control module is triggered when the first water level detection device detects that the water level height in the first cavity reaches a first preset water level, the control module controls the infusion pump to adjust the current first rotating speed to a second rotating speed, and the second rotating speed is larger than the first rotating speed. According to the cleaning equipment, separation of solid dirt and dirty liquid is effectively achieved, cleaning of the sewage tank is facilitated, and the risk that the sewage tank is prone to breeding bacteria and generating peculiar smells is reduced to a certain degree.

The application belongs to the technical field of microorganisms, and particularly discloses application of Bifidobacterium longum subsp. longum CGMCC No. 20105 in bacteriostasis. The application discloses for the first time that the Bifidobacterium longum subsp. longum strain CGMCC No. 20105 has the ability to ferment TPY or MRS to synthesize bacteriostatic substances, and the fermentation liquor has certain inhibitory effect on intestinal pathogenic bacteria, oral pathogenic bacteria, environmental pathogenic bacteria and antibiotic-resistant microorganisms. More importantly, the fermentation product does not affect the survival performance of conventional probiotics, indicating that the bacteriostatic substances synthesized by the Bifidobacterium longum subsp. longum strain CGMCC No. 20105 fermentation have certain safety, specificity and pertinence. Compared with other MRS fermentation strains, the bacteriostatic effect of the MRS fermentation liquor of the Bifidobacterium longum subsp. longum strain CGMCC No. 20105 is remarkable, and therefore has potential application value.

The utility model discloses a mould-proof, insect-proof and waterproof board, which relates to the technical field of boards and comprises a board body, the board body comprises a base material layer, and one side of the base material layer is compositely connected with a waterproof layer. The first cavity is filled with antibacterial particles, the antibacterial property of the plate body can be improved, bacterium breeding can be reduced, compression-resistant strips are fixed in the reinforcing layer, the strength of the plate body is improved, deformation of the plate body is reduced, reinforcing strips are arranged in the aromatic layer, a plurality of second cavities are formed in the reinforcing strips, and the antibacterial property of the plate body can be improved. And the second cavity is in a honeycomb shape, the strength of the board body can be improved, the second cavity is filled with the insect repellent, special smell can be released, the repelling effect on common wood pests is achieved, and therefore the board is effectively protected from being corroded by the pests.

This invention discloses a residual liquid removal structure and method for an eye dropper, comprising: a bottle body, a dropper shell, a dropper column, and an annular sealing gasket. The dropper shell is connected to the bottle body and has a flow channel. An outlet is located at the top of the flow channel, and an annular sealing gasket is located at the connection between the outlet and the flow channel. The dropper column is located within the flow channel of the dropper shell, and includes a T-shaped section and a conical section. The conical section passes through the annular sealing gasket and the outlet and extends outward from the dropper shell. The inner ring of the annular sealing gasket is sealed to the outer wall of the conical section. Several guide grooves are evenly distributed on the dropper column. The advantages of this invention are: after dispensing, releasing the bottle body pushes the liquid film adhering to the conical section of the dropper column and the liquid suspended at the end of the conical section away from the conical section, allowing it to be dispensed into the user's eye without waste. Furthermore, it keeps the conical section exposed to air dry, thus preventing bacterial growth.

Antimicrobial eyewear features frames infused with or coated with antimicrobial additives, thereby offering continuous protection by actively destroying and inhibiting the growth of bacteria, fungi, parasites, and some viruses. The lenses may also have an antibacterial coating that destroys and inhibits bacterial growth. In some examples, the lenses are treated with a specialized strengthening liquid, wherein the liquid contains nano components that release heavy metal ions capable of invading the cell walls of bacteria, leading to bacterial elimination, destroying DNA molecules and proteases within the bacterial cells, denature viral proteins, break DNA chains, and result in cell death. Additionally, the strengthening liquid reduces dehydrogenase activity and interacts with various protein groups within the cell, thereby reducing the activity of these groups and effectively inhibiting the growth of E. coli and other bacteria on the lens surface.

The present utility model provides a gas purification device. The gas purification device comprises a main machine assembly and a filter assembly. The main machine assembly comprises a machine body and a rotating member rotationally arranged in the machine body, and the rotating member is arranged at a lower opening of the machine body. The filter assembly comprises a dust collection cylinder and a cyclone cone arranged in the dust collection cylinder; the dust collection cylinder is connected to the lower part of the machine body; the side wall of the dust collection cylinder is provided with an air inlet; a first airflow cavity channel is formed between the dust collection cylinder and the cyclone cone; the cyclone cone is provided with a filter channel in communication with the lower opening of the machine body, and is provided with a filter screen assembly for enabling the filter channel to be in communication with the first airflow cavity channel; the cyclone cone is used to enable gas entering the first airflow cavity channel through the air inlet to form a cyclonic airflow when the rotating member rotates, the cyclonic airflow flowing to the lower opening of the machine body through the filter channel. In this way, the cleaning interval of the filter screen assembly is effectively extended, the service life of the filter screen assembly is prolonged, and the cleaning efficiency of the filter screen assembly is improved, and the risk of bacterial growth in the filter screen assembly is reduced.

This utility model relates to a modular ventilator, comprising a main unit, a heating component, and a water tank assembly. The main unit and the heating component are detachably connected via electrical terminals, and the heating component and the water tank assembly are detachably connected via a snap-fit ​​and snap-fit ​​slot unit. Additionally, another snap-fit ​​and snap-fit ​​slot unit is included to prevent the main unit from detaching from the heating component. This utility model is easy to carry and use, especially suitable for outdoor use. It also facilitates user disassembly, cleaning, and maintenance of the water tank, reducing bacterial growth. Furthermore, the modular structure supports functional expansion, allowing users to replace or upgrade components as needed, thus broadening its applicability.

The invention discloses a biochar composite membrane and a selective denitrification method and application thereof, and belongs to the field of sewage treatment.The preparation method comprises the steps that biochar, polystyrene and polydimethylsiloxane are dispersed in an organic solvent to form dispersion liquid, the dispersion liquid is applied to the surface of a substrate, and then curing and drying are conducted to obtain the biochar composite membrane. A biochar composite film is obtained; geobacillus stearothermophilus is inoculated on the surface of the obtained biochar composite membrane, and incubation is carried out under the condition suitable for growth of bacteria until a microbial membrane of the geobacillus stearothermophilus is formed on the surface of the biochar composite membrane; transferring the obtained biochar composite membrane with the microbial membrane formed on the surface into one or more porous suspension balls, and then placing the porous suspension balls on the surface of sewage. The method can be applied to outdoor sewage in-situ efficient selective denitrification, and is more economical, energy-saving, green and environment-friendly.

The invention relates to the technical field of biological medicine, in particular to a fibroblast-targeting phycocyanin conjugate, a sol preparation and application of the fibroblast-targeting phycocyanin conjugate, phycocyanin and targeting peptide are covalently linked through an SMCC cross-linking agent, LDL receptor-associated protein 1 highly expressed by fibroblast is specifically recognized through the targeting peptide, and efficient intracellular delivery is achieved; a water-soluble sol preparation carries phycocyanin to break through a cell barrier, and LRP1-rich fibroblasts in a wound are targeted and play a role in the fibroblasts. By inhibiting the EMT process of fiber cells, cell fibrosis and growth of wound bacteria are inhibited, scar formation is reduced, and wound healing is promoted. The defect that natural phycocyanin cells are poor in penetrability is overcome, scar-free healing is achieved by regulating Wnt5a / beta-catenin through targeted delivery, the effect is good, and application and popularization are convenient.

The present invention belongs to the field of pharmaceutical technology and relates to a preparation that binds to the AbaR protein of Acinetobacter baumannii and its application. The present invention utilizes sansinol O to effectively bind to the AbaR protein in Acinetobacter baumannii, thereby inhibiting its AHL quorum sensing system. It has been confirmed that sansinol O can significantly inhibit the biofilm formation ability of Acinetobacter baumannii ATCC 19606 strain, reduce bacterial motility, and reduce bacterial toxicity to cells, without significantly affecting bacterial growth and causing selective pressure to promote bacterial evolution of drug resistance.

This utility model belongs to the field of water treatment technology, specifically relating to a water dispenser and equipment for pharmaceutical production using a full membrane process. The water dispenser includes: a water pretreatment system suitable for treating raw water into drinking water, and includes: a filtration module and a sterilization module; the filtration module includes: a first buffer tank suitable for receiving and conveying raw water; a first dosing unit suitable for adding chemicals to the raw water conveyed from the first buffer tank; and a filtration unit equipped with a nanofiltration membrane, suitable for receiving the raw water conveyed from the first buffer tank, filtering it through the nanofiltration membrane, and returning it to the first buffer tank to form a cycle, and suitable for conveying the filtered raw water to the sterilization module after a corresponding cycle time; the water pretreatment system treats the raw water into drinking water, and the water treatment system treats the drinking water into water for injection, and a first loop pipe keeps the water flowing during the treatment process to prevent bacterial growth.

An integrated biofilter for a re-circulating aquaculture system is provided. The biofilter may include a moving bed biofilm reactor (MBBR) with biomedia elements configured to support bacteria growth and nitrification of the wastewater and an MBBR output configured to output biomass generated by the bacteria. The biofilter may also comprise a periphyton biofilter configured to introduce oxygen into the wastewater, and a halophyte biofilter comprising a plurality of plants. The halophyte biofilter configured to capture nutrients in the wastewater to generate edible plant mass.

This invention discloses a pharmaceutical composition containing the natural products osthol and berberine, exhibiting synergistic antibacterial and antifungal effects. The effectiveness of osthol in synergistically enhancing the antibacterial activity of berberine was demonstrated through checkerboard minimum inhibitory concentration (MIC) tests, antifungal activity experiments, and in vitro bacterial growth curves. This invention provides a novel application of the combined use of osthol and berberine in antibacterial and antifungal treatments. This composition belongs to the category of traditional Chinese medicine compound preparations, offering a new treatment strategy for the clinical treatment of bacterial and fungal infectious diseases.