4 results about "Ribosomal RNA" patented technology

This invention discloses a compound seed treatment agent containing metalaxyl-M and tebuconazole, its preparation method, and its uses, belonging to the technical field of seed treatment agents. The suspension of this invention uses metalaxyl-M, tebuconazole, and sodium selenite as the main components, supplemented with emulsifying and dispersing agents, wetting and dispersing agents, antifreeze agents, suspending agents, film-forming agents, and warning colors, and is prepared by adding deionized water to a sufficient volume. Metalaxyl-M targets and inhibits ribosomal RNA polymerase I of oomycete pathogens, while tebuconazole targets and inhibits sterol 14α-demethylase. The two have complementary fungicidal spectra, synergistically controlling maize stalk rot and maize head smut. Sodium selenite, by inhibiting the oxidative stress defense system of pathogenic fungi involving glutathione and thioredoxin, promotes the effects of the two main components and simultaneously remediates selenium-deficient soils. This invention is suitable for the green and efficient control of maize stalk rot and head smut, and also has the function of ecological restoration of selenium-deficient soils.

The application belongs to the technical field of fluorescent probes, and particularly relates to a preparation method and application of an RNA fluorescent probe based on biological orthogonal reaction, and the structural formula of the fluorescent probe is: the application provides a preparation method for generating an RNA activated near-infrared fluorescent probe based on biological orthogonal reaction. First, (E)-2-chloro-3-(hydroxymethyl)cyclohex-1-en-1-methanal and 6-bromo-1-ethyl-2-methylquinolin-1-ium salt are used as raw materials to synthesize a precursor compound beta-Br, and then the beta-Br is rapidly in-situ generated into a fluorescent probe under physiological conditions. After the fluorescent probe generated based on biological orthogonal reaction is reacted with RNA, the fluorescence intensity at the near-infrared region 670-900 nm is enhanced with the increase of the RNA concentration, and the fluorescence enhancement multiple reaches 400. Secondly, the fluorescent probe generated based on biological orthogonal reaction shows high selectivity to RNA, and is not affected by other various ions, active oxygen, RNA bases, biological thiols and amino acids. Finally, the fluorescent probe can be washed-free and fast imaging in living cells, and can be in-situ fast generated into a fluorescent probe in cells, and can be used for monitoring intracellular ribosomal RNA, distinguishing between living cells and dead cells, and real-time monitoring of intracellular oxidative stress.

A method for identifying exogenous viruses based on metagenomic sequencing, comprising the following steps: S1, a data quality control step, filtering the quality of the metagenomic sequencing raw data of the sample to obtain clean sequencing data; S2, a host removal step, aligning the clean sequencing data with a host reference genome and a ribosomal RNA database, removing the host sequences on the alignment, and obtaining enriched microbial sequences. The method integrates kraken2 species identification, RVDB / nt_core database alignment, virsorter2 / checkV verification and other multiple links to form a multi-dimensional identification system, improve the accuracy and reliability of virus identification, introduce a multi-process parallel processing mechanism to support the synchronous analysis of batch samples, greatly improve the analysis efficiency, adapt to the high-throughput detection needs of large-scale cell banks, biological products intermediates and finished products, and solve the problem of low throughput of traditional methods and existing mNGS methods.

The application discloses OsRNE A gene and application of the gene and a coded protein in rice leaf color regulation and chloroplast development. OsRNE The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the coded protein is shown as SEQ ID NO. 2. OsRNE The application utilizes CRISPR / Cas9 gene editing technology to knockout the gene and obtain a loss-of-function mutant. The mutation of the gene leads to impaired chloroplast development of rice, affects chlorophyll synthesis, albino of seedlings and death within three-leaf stage, and thus can be used as a marker trait to assist rice molecular breeding at the seedling stage. OsRNE The gene is mainly expressed in leaves, the coded protein is located in chloroplasts, and the chloroplast development and normal growth of seedlings are affected by affecting the metabolism of ribosomal RNA in chloroplasts. Therefore, the application can be applied to molecular genetic breeding of rice leaf color traits, and has important significance for further understanding of a chloroplast development regulation mechanism of rice and yield increase of rice.