Multiple-gene detecting kit related to antitumor drugs

Abstract

The invention relates to a multiple-gene detecting kit related to antitumor drugs. The multiple-gene detecting kit comprises a mixture of RT (reverse transcription) primers and a mixture of PCR (polymerase chain reaction) amplification primers, wherein each RT primer and each PCR amplification primer based on genetic groups, reference genes and a reaction internal label are respectively contained in each of the mixture of the RT primers and the mixture of the PCR amplification primers; the multiple-gene detecting kit is characterized in that the genetic groups comprise PTEN, EGFR, DPYD, HER2, RRM1, ERCC1, TUBB3, TOP1, TYMS and TOP2A; the reference genes comprise ACTB, GAPDH and B2M; the reaction internal label is KAN-rRNA (ribosomal RNA). The multiple-gene detecting kit related to antitumor drugs disclosed by the invention can be used for systemically detecting the expression level of a plurality of genes closely related to the antitumor drugs by one step, is simple and convenient to use, good in accuracy and high in detecting efficiency, and can be used for guiding chemotherapy drugs and selecting chemotherapy regimens very well.

Description

technical field [0001] The invention relates to a gene detection kit, in particular to a kit for synchronous detection of gene expression levels related to multiple anti-tumor drugs, belonging to the technical field of medical molecular biology. Background technique [0002] As we all know, chemotherapy is the most commonly used option in cancer treatment today, but more than half of the patients often have little curative effect after chemotherapy, and the same tumor drug often has great differences in the curative effect for different patients; at the same time, the side effects of chemotherapy drugs are relatively high. Large, it kills tumor cells and normal tissue cells at the same time, and sometimes complications occur. Many patients are tortured and even die due to the strong toxic side effects of chemotherapy. A large number of clinical studies have confirmed that the treatment of tumors based on experience alone generally has a curative effect of less than 30%, and ...

AI Technical Summary

Problems solved by technology

However, if it is necessary to detect the expression of multiple genes, it is mostly necessary to detect each gene one by one, and it is difficult to set an internal reference gene, with low throughput, high cost, and poor convenience.

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