95results about How to "Easy to standardize operation" patented technology

The invention discloses an expanding propagation method for aphidius gifuensis ashmeads. The method comprises the following steps: inoculating aphidius gifuensis ashmead females which are about to spawn on rape seedlings with myzus persicaes, conducting aphidius gifuensis ashmead cultivation, collecting the aphidius gifuensis ashmeads, and achieving expanding propagation. According to the expanding propagation method for the aphidius gifuensis ashmeads, myzus persicaes serve as hosts of aphidius gifuensis ashmead propagation, optimized aphidius gifuensis ashmead inoculating ratio, optimized inoculating opportunity, aphidius gifuensis ashmead collecting are involved, a parasitic wasp expanding propagation technology of being high-efficiency, economical and easy and convenient to apply is formed through repeated exploration and improvement, and therefore the purposes of biological preventing and treating injurious insects are achieved.

The invention provides a four-station four-axis turning tool sharpening CNC machine tool, comprising a machine bed body, a grinding wheel, an electric tool rest, a grinding wheel driving device and an electric knife rest driving device are arranged on the machine tool bed, and the electric knife rest driving device includes Longitudinal driving device and horizontal driving device; the longitudinal driving device is used to drive the electric tool post for longitudinal movement; the horizontal driving device is used to drive the electric tool post for horizontal movement; The straight rotary drive device is used to drive the electric tool post for vertical rotary motion; the grinding station conversion device is used to drive the electric tool post to change the grinding station; it can realize the fast and fully automatic operation of the grinding process, reducing the operator's experience. Insufficient level and the impact on the quality of turning tool sharpening, it is convenient to realize the standardized operation of turning tool sharpening, it can realize the automatic grinding of four turning tools in sequence, and the work efficiency is high.

The invention relates to a micro-satellite instability multi-detection system and a kit. The detection system comprises a universal-specific gomphosis primer pair and a mixture of universal primers, wherein the universal-specific gomphosis primer pair respectively aims at mononucleotide micro-satellite loci BAT-26, BAT-25, NR22, NR24 and NR21; the universal primer is Flu-UP; a sequence of Flu-UP sequence is arranged at each upstream primer 5' end of the universal-specific gomphosis primer pair; and the universal primer Flu-UP is a fluorescence labeling primer. By adopting the micro-satellite instability multi-detection system and the kit provided by the invention, the defect that different primers need multiple fluorescence labels is overcome, 5 micro-satellite gene loci can be detected in one same PCR (Polymerase Chain Reaction) reaction system, the result is visible, reliable, time-saving and efficient, the cost is saved, and the MSI (Micro-satellite Instability) gene loci can be effectively detected and analyzed.

The invention discloses an indoor breeding method for drosophila-suzukii-matsumura advantageous parasitic wasps trichopria drosophilae.The indoor breeding method includes the following steps that drosophila pupas in the age of 1 day to 2 days are fed and collected, trichopria drosophilae is parasitized to the drosophila pupas (female trichopria drosophilae and male trichopria drosophilae copulate according to the proportion of 1:1), the trichopria drosophilae is collected, and the indoor breeding procedure is completed.The provided expanding propagation method for the trichopria drosophilae is convenient, economical and prone to standard operation, the trichopria drosophilae can be rapidly and largely subjected to expanding propagation, the technological base is provided for industrial production, and the aim for biological control over insect pests is achieved.

The invention relates to a compound amplification system and a kit for detecting chromosome deficiency. The compound amplification system comprises a mixture of a universal-specific chimeric primer pair and a universal primer, wherein the universal-specific chimeric primer pair respectively aims at genetic locus of D1S468, D1S436, D1S489, D19S112, D19S217 and D19S902. The universal primer is FluD5-Up. A sequencer of FluD5-Up is arranged at the 5' end of each forward primer in the universal-specific chimeric primer pair, and the FluD5-Up is a fluorescence labeling primer. The compound amplification system and the detection kit provided by the invention overcome the deficiency that various fluorescence labels are required for different primers, and 6 1p19q genetic locus can be detected once by using a single fluorescence label in a PCR (Polymerase Chain Reaction) system, so that the system is intuitive and reliable in result and time-saving and efficient, and can effectively detect and analyze loss of heterozigosity of the chromosome 1p19q.

The invention belongs to the technical field of medicament analysis, and particularly relates to a qualitative and quantitative method of 3-aminopiperidine as well as a detection method of chiral purity of 3-aminopiperidine. According to the invention, excessive benzoyl chloride is adopted as a derivatization reagent to be subjected to rapid bi-derivatization reaction with 3-aminopiperidine so as to generate diphenyl 3-aminopiperidine, and then common phase reversal HPLC-UV(high performance liquid chromatography-ultraviolet) is used for analyzing diphenyl 3-aminopiperidine qualitatively and quantitatively; Chiral HPLC Columns taking glycoprotein as a filling are adopted for determining the enantiomeric excess rate of diphenyl 3-aminopiperidine. The method is simple and rapid to operate, is good in reproducibility, is high in flexibility, and is easy for standardized operation.

The invention provides a composite beehive and a manufacturing process thereof. The composite beehive comprises a plurality of stacked beehive bodies, a beehive bottom plate, covering lids and a top lid, wherein the covering lids and the top lid are arranged on the beehive bodies. Each beehive body is composed of beehive body side plates in a four-side surrounding mode, wherein the beehive body side plates comprise a pair of outer plates and a core plate arranged between the outer plates, the core pate is a closed-cell foam plate made of polyurethane material, and the outer plates are made of glassfiber reinforced plastic. The product is formed through the processes of moulding, laying, hot-pressing and the like. As the core plates are of a closed-cell structure, an excellent mechanical property is achieved while an excellent heat preservation and heat insulation effect is brought, and the weight of the whole beehive is reduced to a great extent; as an internal and external wall material of the composite beehive is quite stable in chemical property, beehive ageing or rotting caused by long-term exposure to the sun and rain can be avoided; as the internal and external wall material is a non-biological material, termite gnawing can be avoided; the composite beehive is low in production cost and high in standardization degree.

The invention relates to the technical field of building construction and particularly relates to an interior wall wallpaper pasting device applied to building construction. The interior wall wallpaper pasting device comprises a horizontal moving base, sliding guide rods vertically and movably inserted on the upper surface of the horizontal moving base, wherein one side corresponding to a pasting operation shell of a mounting bottom plate is an opening; an operation main body blocking cover is arranged in the opening; the pasting operation shell, the mounting bottom plate and the operation main body blocking cover jointly form an operation sealed space; a glue dipping drum wheel set, a wallpaper placing cylinder, a first guide cylinder and a second guide cylinder are arranged on a positioning bearing in a rolling manner. The interior wall wallpaper pasting device can be disassembled and assembled through the sliding guide rods and the horizontal moving base; convenience in transportation and carrying is achieved; meanwhile, two ends of a transverse positioning rod are in sliding connection with sliding grooves; with cooperation of opposite force action of weight balancing blocks, the transverse positioning rod runs more stably; the device also has functions of automatically applying glue to wallpaper and walls, so that the purpose of one-man operation is achieved; furthermore, the device is also capable of positioning reference according to positions of the walls and has relatively high accuracy.

The invention relates to an automatic cleaning and disinfecting system and cleaning and disinfecting method for milk cows. The automatic cleaning and disinfecting system comprises a cow herd transferring passage, a to-be-milk region, a disinfecting passage, a cleaning and disinfecting system, a sick cow treatment passage, a milking region, a return passage and a disinfecting system, wherein the cow herd transferring passage communicates with one end of the to-be-milk region, and the other end of the to-be-milk region communicates with one end of the disinfecting passage; the cleaning and disinfecting system is arranged on the disinfecting passage; one side of the cleaning and disinfecting system communicates with one end of the sick cow treatment passage, and the other end of the disinfecting passage communicates with the milking region; one end of the return passage communicates with the milking region, and the disinfecting system is arranged on the return passage; and the other end of the return passage communicates with cow herd transferring passage. The cleaning and disinfecting system consists of a front gate, a rear gate, a separation door, a door control module, a steel frame support structure, a wireless transferring module, a readcard module, a mechanical arm, a mechanical executing and control module, an image collecting and treating module and cleaning and disinfecting units. The automatic cleaning and disinfecting system disclosed by the invention is simple and convenient to operate, the labor cost for milking is effectively reduced, and the safety is improved.

The invention provides a filling mining method for a fully mechanized top coal caving working face, and relates to the technical field of mining engineering. The technical problems of control over a roof of the fully mechanized top coal caving working face and large deformation of a top coal caving mining surface are solved. The filling mining method comprises the steps that A, the fully mechanized top coal caving working face is divided in the extending direction of the working face into a filling area and a top coal caving area, or the working face is divided in the extending direction and the inclination direction into a filling area and a top coal caving area; B, a working face cycle step pitch is determined; C, the filling area in front of the working face is supported, and when coalcaving of the top coal caving area is completed, round wood supports are erected in the filling area; D, after the filling area reaches a filling step pitch, a filling tarpaulin is arranged behind a hydraulic support, and filling paste is pumped; and E, the steps C and D are repeated to complete the mining. The mining method of alternating coal caving and filling is provided to complete filling and mining of the top coal caving working face, a roof is effectively controlled, surface subsidence is reduced, and the method further has the advantages such as safety and high efficiency.

The invention relates to the field of tealeaves processing, in particular to a method for increasing the productive rate of theaflavoin formed by converting catechinic acid in fresh tealeaves. The adopted scheme is as follows: storing harvested fresh tealeaves into a soft sealed bag, carrying out vacuum sealing on the bag (controlling the vacuum degree at minus 0.1MPa to minus 0.01MPa), adequately freezing the fresh tealeaves, warming the tealeaves under an ambient temperature which is more than zero (about 4 to 37 DEG C) for 2 to 15h, and promoting the catechinic acid to be enzymatically synthesized into theaflavin. After the reaction is ended, the high-temperature withered fresh tealeaves can be directly processed into bar-shaped red tea, flat red tea or twisted into coiled red tea; the mass percent of four theaflavin monomers (theaflavin, theaflavin-3-gallate, theaflavin-3'-gallate and theaflavin-3,3'-bi-gallate) in a dry sample of the prepared read tea is more than or equal to 2 percent of the total mass percent.

The invention relates to a multiple-gene detecting kit related to antitumor drugs. The multiple-gene detecting kit comprises a mixture of RT (reverse transcription) primers and a mixture of PCR (polymerase chain reaction) amplification primers, wherein each RT primer and each PCR amplification primer based on genetic groups, reference genes and a reaction internal label are respectively contained in each of the mixture of the RT primers and the mixture of the PCR amplification primers; the multiple-gene detecting kit is characterized in that the genetic groups comprise PTEN, EGFR, DPYD, HER2, RRM1, ERCC1, TUBB3, TOP1, TYMS and TOP2A; the reference genes comprise ACTB, GAPDH and B2M; the reaction internal label is KAN-rRNA (ribosomal RNA). The multiple-gene detecting kit related to antitumor drugs disclosed by the invention can be used for systemically detecting the expression level of a plurality of genes closely related to the antitumor drugs by one step, is simple and convenient to use, good in accuracy and high in detecting efficiency, and can be used for guiding chemotherapy drugs and selecting chemotherapy regimens very well.

The invention discloses a trapping device and specific application method to estimate trapping ability for natural enemy of rice louse in the biological prevention construction studying technological domain of crop pest natural enemy, which comprises the following parts: column (1), fixing coil (2), fixing bolt (3), transverse column (4), culture plate ring (5), culture plate (6), drinking cup (7), drinking cup (8) and screen (9).

The invention discloses a production method of conditioned chicken claws. The method comprises the steps of (1) boiling chicken claws in a water bath at 80-90 DEG C for 4-6 minutes, fishing out and flushing with cold water; (2) soaking the chicken claws obtained in the step (1) in a mixed liquid of acetic acid and lactic acid for 4-6 hours, and washing, wherein the ratio of weight of the chicken claws and volume of the mixed liquid is 1:4-1.8g / mL; (3) mixing and pickling the chicken claws obtained in the step (2) and a pickling liquid according to a mass ratio of 1:1.5-1:2.5 for 3-6 hours; and (4) sterilizing to obtain the conditioned chicken claws. According to the product obtained by the method provided by the invention, the process flow is simple and convenient to operate in a standardized manner. Compared with the production process of conventional fermented pickled chicken claws, the output time of the product is greatly shortened, and the yield as well as the appearance and the volume of the product can be increased by an acidizing and crisping operation, so that the production efficiency is increased.

The invention discloses an industrialized production method for nesidiocoris tenuis, and belongs to the technical field of biological control over agricultural pests. The method comprises the steps of legume crop cultivation, sterilization of glasshouse soil of rice moth eggs and nesidiocoris tenuis raising and collecting. According to the industrialized production method for the nesidiocoris tenuis, a series of technical problems such as plant source and animal source feed combination, plant oviposition materials and raising media are solved successfully, the problems that at present, the nesidiocoris tenuis can only be raised on a small scale in a laboratory, and the control requirements in actual production cannot be met are changed, and the industrialized production efficiency of the nesidiocoris tenuis is greatly improved. By taking the nesidiocoris tenuis as a predatory enemy to be applied to vegetables in a large area, the number of use and the use amount of insecticide can be greatly lowered, an agricultural ecological environment is protected, and the food safety is improved.

The invention discloses a minimally invasive positioning tool for a laparoscopic surgery and a protected supporting and positioning method of the tool, and relates to a minimally invasive positioning tool and a minimally invasive surgery method. A pneumoperitoneum operation mode of an existing laparoscopic surgery is very harmful to human bodies and large in wound, surgical difficulty and dangerousness are increased, other pneumoperitoneum modes are very harmful to patients and cause many complications, and standard operation is difficultly realized as an affected part of an abdominal cavity needs to be cut by the aid of experience of a doctor due to a narrow surgical field. A unilateral slide can be connected with a detachable base, a movable base is in sliding fit with the unilateral slide, a supporting component is arranged on the movable base, a buffer type guide head is arranged at one end of an intubation tube, a gas filled bag is arranged inside the intubation tube and communicated with a gas source, and a plurality of slot holes are formed in the intubation tube. The protected supporting and positioning method is characterized in that the gas source is started, gas flow with a gas pressure value P is led into the gas filled bag of the intubation tube, and the gas filled bag is filled with gas to form multi-point supporting or arc-shaped supporting. The minimally invasive positioning tool and the protected supporting and positioning method are used for the laparoscopic surgery.

The invention provides a formula of konjak cake. According to the formula, the konjak cake comprises eggs, white sugar, honey, cake oil, cake flour, baking powder, fragrant powder, water, konjak flour, salt and soybean oil. According to a preparation method of the konjak cake, refined konjak flour is added on the basis of the traditional cake preparation, and by research and improvement on the reasonable formula, the konjak cake produced not only can provide basis for developing the cake towards the nutritional and health-care type direction, but also can be used as a health-care food for healthy people, sub-healthy groups and patients with obesity and the like.

Belonging to the technical field of pharmaceutical analysis, the invention specifically relates to an analysis method for colistimethate sodium. With simple operation, good reproducibility, high sensitivity and accuracy, as well as easy standardized operation, an analysis method for components of colistimethate sodium, or in other words, a composition analysis method for components of colistimethate sodium is provided. The analysis method is characterized in that: a colistimethate sodium solution is converted to a colistin solution by means of pyrohydrolysis after acidification, and then components of colistin are employed to represent components of colistimethate sodium.

A uterine cervix sampler for sampling from the mucosa on the surface of uterine cervix is composed of brush head, brush handle and connecting mechanism between brush head and brush handle. Said brush head has E-letter shape consisting of two L shapes, on which there is a sampling material.

The invention discloses a method for simultaneous detection of contents of three isomers in palonosetron hydrochloride. The method includes following steps: (1) preparation of a mobile phase: mixing n-hexane with alcohol according to a volume ratio of (60-80):(40-20), adding a certain amount of trifluoroacetic acid and diethylamine, and obtaining the mobile phase; (2) preparation of a test solution: weighing the palonosetron hydrochloride, and preparing a solution with the concentration of 0.1-10 mg / ml through solvents; and (3) detection: detecting a sample by employing a high-efficiency liquid chromatography ultraviolet detector, wherein the ultraviolet detection wavelength is 230-250 nm, and a chromatographic column is a chiral column regarding amylose tris(3,5-dimethylphenylcarbamate) as a filling agent. According to the detection method, the operation is simple, the separation of major peaks and the isomers is good, the reservation is moderate, the sensitivity is high, it is verified that the accuracy and the precision of the analysis method are high, the specificity and the reproducibility of the method are good, and standardized operation is facilitated.

The invention discloses a new application of Chromatomyia horticola in propagation of Diglyphus isaea. The new application of Chromatomyia horticola provided by the invention is the application of Chromatomyia horticola in propagation of Diglyphus isaea. The application comprises the following steps: inoculating spawning female Diglyphus isaea on plant seedling infected with larva of Chromatomyia horticola, performing cultivation, and collecting Diglyphus isaea to realize propagation. The application takes Chromatomyia horticola as host; repeatedly researches and improves in technical links of optimal inoculation proportion, key inoculation time, adult Diglyphus isaea collection and storage, etc.; and forms a high-efficiency, economic and simple parasitic wasp propagation technology, to rapidly propagate Diglyphus isaea in large scale, thereby realizing bio-control of pests.

The invention relates to a liver cancer tumour marker AFP, AFP variant and GP73 antigen synchronous immunodetection kit, a method and application, and belongs to the technical field of immunodetection. The kit comprises magnetic particles, AFP and GP73 antibody coupled mixed acridinium ester, GP73, an AFP calibration product, first stimulation solution, second stimulation solution, washing solution and all kinds of diluting solution; and the magnetic particles are respectively marked by AFP, LCA-BSA and GP73. By adoption of the kit and the detection method, the sensitivity, the specificity andthe accuracy are high; the detection time is short; identification on benign liver diseases and malignant liver diseases and early diagnosis on liver cancers can be easily carried out; and the kit and the detection method have an important purpose for increasing the early diagnosis rate of patients with liver cancers and prolonging the survival rate, and have wide clinical application value.

The invention relates to a multi-gene detection kit for the selection of chemotherapy regimens. The multi-gene detection kit comprises a mixture of RT (reverse transcriptase primer) primers and a mixture of PCR (Polymerase Chain Reaction) amplification primers, wherein the mixture of RT primers and the mixture of PCR amplification primers comprise each RT primer and each PCR amplification primer based on genetic groups, reference genes and an internal standard substance of the reaction respectively, also comprise random primers, the genetic groups comprise RRM1, TOP2A, ERCC1, TYMS, TUBB3, TOP1, PTEN, HER2, DPYD and EGFR; the reference genes comprise ACTB, GAPDH and B2M; the internal standard substance of the reaction is KAN-rRNA. The multi-gene detection kit for the selection of chemotherapy regimens, which is disclosed by the invention, can be used for simultaneously detecting the expression levels of multiple genes closely related to anti-tumor medicines systematically, and is easy to use, good in accuracy, high in detection efficiency and can be well used to guide chemotherapy medication and select chemotherapy regimens.

The invention relates to an HPLC measuring method for three ingredients of trinary mycin for injection. The HPLC measuring method comprises the following steps: 1), preparation of a reference solution; 2), preparation of a test solution; 3), chromatographic conditions, wherein the chromatographic column is a C18 reversed-phase chromatographic column, the mobile phase is a buffer salt solution-acetonitrile of which the volume ratio is 81:19, the buffer salt solution contains 0.21 mol / L of monopotassium phosphate and 0.16 percent of triethylamine, the pH value is 5.6, the flow velocity is 1.0 ml / min, the detection wavelength is 215 nm, and the sample size is 10 [mu]l; 4), content calculation, wherein the content calculation method adopts the external standard method, and the contents of procaine, dibenzyl ethylene diamine and penicillin in a test sample are calculated according to peak areas. According to the HPLC measuring method, the contents of the three ingredients of the trinary mycin can be measured at the same time, and the HPLC measuring method has the advantages of high repeatability, sensitivity and accuracy, and facilitates standard operation.

The invention provides an inoculation method suitable for medical bombyx batryticatus production. The inoculation method comprises the following steps of a, early breeding, wherein mulberry leaves areused as food, and bombyx mori linnaeus is artificially raised; regular feeding with the mulberry leaves, wherein the bombyx mori linnaeus is fed with the mulberry leaves 3-4 times every day; screen arrangement for litter cleaning; treatment before sleep; b, inoculation, wherein a beauveria bassiana working solution (1*10<6>-1*10<8> heads / m<2>) is prepared by using sterile water containing 0.05% of Tween 80 at the temperature of 23-28 DEG C, and inoculation is conducted. By means of the inoculation method suitable for medical bombyx batryticatus production, the deadweight of the bombyx batryticatus is high, the living number of the bombyx batryticatus is small, the yield is high, standard operation is facilitated, and the inoculation method is suitable for industrial mass production.

The invention discloses a chemiluminescent quantitative detection kit for a blood plasma HSP70 (heat shock protein) antibody, belonging to the field of external clinical inspection and chemiluminescent immunodetection. The kit comprises a blood plasma HSP70 antibody detection reaction plate which is coated with a recombined HSP70 antigen; the quantitative detection kit for the plasma HSP70 antibody further comprises an enzyme conjugate, a luminous substrate, a calibration material, a quality control substance and concentrated washing liquid. The kit disclosed by the invention can exclusively and quantitatively detect the content of the blood plasma HSP70 antibody; compared with the conventional ELISA (enzyme-linked immuno sorbent assay) technology, the chemiluminescent quantitative detection kit has the advantages that the high specificity of the ELISA technology, and the stability, the reliability and the operation convenience of a detection result are retained, and meanwhile, by the adoption of a chemiluminescent method, the detection sensitivity is improved.

The invention provides a method for controlling temperature and humidity for a culture environment. According to the method, the inoculation environment is controlled at 24-28 DEG C and 85-95% RH, a rearing shelf with the area being not less than 30m<2> is needed to be prepared for five-age silkworms per paper, the culture environment is controlled at 24-28 DEG C and 70-80% RH, debris is removed,and ventilation for air exchange is performed once every 6 hours. The invention also provides a breeding method of high-quality and high-yield medicinal bombyx batryticatus. The breeding method comprises the following steps: a, pre-culture: regularly supplying mulberry leaves, screening and removing sand, and performing pre-sleep treatment; b, inoculation environment: adjusting the temperature andhumidity of an inoculation room, and performing ventilation for air exchange; c, inoculation: performing spray inoculation three times within 0 to 16 hours after silkworm forming; d, spacing: spacingday by day, wherein the area of the rearing shelf is not less than 30m<2>. The method provided by the invention is suitable for artificially large-scale breeding of medicinal bombyx batryticatus, theyield is high and the quality is good.

The invention relates to a medical instrument special for clyster in nephrology departments, in particular to a clyster vehicle special for the nephrology departments. The clyster vehicle comprises a vehicle body, a clyster bag accommodating box (1) is arranged on the upper portion of the vehicle body, a lower-layer locker (5) is arranged on the lower portion of the vehicle body, supporting plates (2) are arranged on opposite inner walls of the clyster bag accommodating box (1), and one end of each supporting rod (3) is fixed on the corresponding supporting plate (2) while the other end of the same is suspended. Every two opposite supporting rods (3) positioned on the corresponding side walls of the clyster bag accommodating box (1) are connected through bent connecting pieces (301). One end of each connecting piece (301) is hinged to the suspended end of the corresponding supporting rod while the other end of the same is in overlap joint with another supporting rod. A plurality of grooves (302) used for accommodating colon dialysis fluid medicine bags are formed in the supporting rods (3). By the clyster vehicle, a standard and convenient process of conveying clyster bags is realized, and working efficiency of medical staff is effectively improved.

The invention discloses a small-molecular compound combination and a method for inducing differentiated cells to prepare chondrocytes by using the small-molecular compound combination. The method is used for preparing the chondrocytes by directional induction of the differentiated cells. The directed induction includes inhibition of the activity of lysine deacetylase, inhibition of a signal pathway of TGF-beta, inhibition of the activity of PKC, inhibition of the activity of DNA methyltransferase, inhibition of the activity of histone methyltransferase, activation of a WNT / beta-catenin signalpathway, activation of a cAMP signal pathway and activation of an RA signal pathway; the small-molecular compound combination includes compounds for regulating and controlling the signal pathways and / or enzyme activities. Through phased induction of the small-molecular compounds, the differentiated cells can be differentiated into the chondrocytes, precision quality control can be achieved in eachstep, and standardization operation and large-scale production are facilitated. The method provided by the invention has wide donor sources, a patient himself can be used as a donor, and the chondrocytes needed for basic research, clinical treatment, or tissue engineering production can be obtained in a relatively short period of time.

The invention discloses a method and device for evaluating a contact state of an ultrasonic probe based on soft tissue morphology. The method comprises the following steps: S11, acquiring ultrasonic information of a detected object to form responses of different contact states; S12, based on features of the soft tissue in the ultrasonic information and boundary features of the soft tissue, identifying a boundary position of the soft tissue in the ultrasonic information; S13, determining a region of interest and a sampling sub-region in the region of interest based on the boundary position and a preset region division condition; S14, extracting morphological parameters of the soft tissue from the selected region of interest; S15, according to the morphological parameters, calculating a forecast value representing the contact state of the probe by using the at least one soft tissue morphological response; and S16, converting the forecast value into an indication signal which changes along with time. The method and the device are based on an existing ultrasonic imaging equipment, and according to the method and the device, a contact state of a probe can be accurately calculated without changing hardware by adopting a software technical means, and operation of an operator is facilitated.