The invention belongs to the technical field of medicines and relates to a percutaneous-absorption palonosetron patch and a preparation method thereof. The patch is composed of a backing layer, a medicine-carrying pressure-sensitive adhesive layer and an anti-sticking layer; the medicine-carrying pressure-sensitive adhesive layer comprises a beta-cyclodextrin inclusion compound of palonosetron free alkali, a pressure-sensitive adhesive and a percutaneous absorption enhancer, wherein the beta-cyclodextrin inclusion compound of the palonosetron free alkali accounts for 4.78%-20.09% of the total weight, preferably 9%-19%, the pressure-sensitive adhesive accounts for 68.80%-95.22% of the total weight and the percutaneous absorption enhancer accounts for 0-11.11wt% of the total weight. In the beta-cyclodextrin inclusion compound of the palonosetron free alkali, the ratio of the palonosetron free alkali to the beta-cyclodextrin inclusion compound is 1: (1-3). The percutaneous-absorption palonosetron patch is provided with the beta-cyclodextrin inclusion compound of the palonosetron free alkali, and therefore, compared with a patch with non-inclusion medicine, the slow release of the medicine within 7 days is realized.