27 results about "Cilium" patented technology

The invention belongs to the technical field of bionic cilium sensors, and particularly discloses a magnetic bionic cilium and vesicle complex and a preparation method and application thereof.The preparation method comprises the following steps that S1, a cilium array male mold is prepared; then carrying out oxygen plasma treatment, silanization and curing stabilization; s3, injecting the magnetic composite precursor into a polydimethylsiloxane female die, applying pressure, and striking off; and S4, coating the mold surface with an Ecoflex precursor, performing thermocuring forming, performing magnetization treatment, and performing stripping to obtain the magnetic bionic cilia. According to the magnetic bionic cilia, the vesicle complex and the preparation method and application of the magnetic bionic cilia, the vesicle complex and the preparation method and application of the magnetic bionic cilia, high-magnetic signal output is achieved while the deformability of the cilia is guaranteed, the magnetic bionic cilia is used for non-contact real-time hemodynamic monitoring, meanwhile, specific response release of the vesicle complex is achieved, and a new thought is provided for design of responsive vascular implants.

The invention relates to a postoperative anti-regurgitation self-cleaning drainage tube which is suitable for chest drainage after pulmonary lobectomy. In order to solve the problems that a traditional drainage tube depends on gravity to conduct passive drainage, a tube cavity is prone to forming a biological membrane due to sediment to cause blockage and drainage efficiency is insufficient, a plurality of cilia are arranged on the inner wall of a drainage tube body in a surrounding mode in the circumferential direction, the sizes of the cilia are gradually reduced from the root to the top end, and the elasticity modulus is reduced in a gradient mode in the drainage direction; and the vibration device is matched to drive the cilia to swing directionally in the same direction as the drainage direction. According to the design, by simulating the respiratory tract cilia function and utilizing the synergistic effect of cilia structural characteristics and vibration excitation, tube wall sediments are actively and physically stripped, a biological membrane is destroyed to form a foundation, and meanwhile, cilia directionally swings to form drainage auxiliary thrust. According to the application, the occurrence rate of lumen blockage can be obviously reduced, the drainage efficiency is improved, the postoperative pain and medical cost of a patient are reduced, the recovery period is shortened, and the clinical efficient drainage and anti-blockage requirements are met.

The present invention relates to a method for producing respiratory organoids by proliferating and differentiating respiratory epithelial cells. [Solution] Unlike conventional respiratory organoids in which epithelial cells present at the apical end of the respiratory tract develop inside the organoid, the respiratory organoid of the present invention has a morphology in which the ciliated structure, which is made up of epithelial cells, is located in the outer layer of the organoid, secretory cells are located inside, and stem cell-like basal cells present at the base of the respiratory tissue are also located inside the organoid. This morphology is similar to that of actual respiratory tissue and has been observed to exhibit normal functions of epithelial cells, such as the motility of ciliated cells. Therefore, by manufacturing organoids using the manufacturing method of the present invention, it is possible to manufacture respiratory organoids that can accurately mimic actual tissue.

The invention relates to application of PDCD5 in preparation of a diagnostic agent or a therapeutic agent for asthenospermia. The invention also discloses a method for constructing an asthenospermia mouse model or inhibiting fibroblast cilia generation by knocking out or knocking down the PDCD5. The application shows that PDCD5 participates in sperm generation, and asthenozoospermia can be caused if the PDCD5 has function deletion mutation in testis. Therefore, the gene locus can be used as a target spot for asthenospermia diagnosis and medication guidance. According to the method disclosed by the invention, the dysfunction of the site is supplemented by the PDCD5 RNA wrapped by the testis injection liposome, so that the asthenospermia caused by the mutation is rescued, the sperm quantity is increased, the sperm malformation rate is reduced, and a new path is provided for the treatment of the asthenospermia.

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a Gli activator in preparation of a product for treating fiberosity. The invention provides an application of a Gli activator in preparation of a product for preventing and / or treating fiber hair disease. The Gli activator comprises an overexpressed Gli adenovirus, an overexpressed Gli adeno-associated virus, a nucleic acid molecule for improving Gli expression and a small molecule compound for activating Gli. The application accidentally finds that the Gli2 adenovirus can remarkably correct the skeleton phenotype of a mouse model of JATD caused by IFT88 deletion, and the activator with the Gli transcription factor as the target spot can promote the expression of a proliferation related gene c-MYC, or n-MYC, or Cyclin D1; the expression of the apoptosis related gene Bcl2 is inhibited; therefore, bone and cartilage cell proliferation is promoted; bone and cartilage cell apoptosis is reduced; and the effect of activating bone and cartilage cells is achieved, so that the fibrinosis is prevented and / or treated, and a potential target and a new treatment scheme are provided for clinical treatment of generalized fibrinosis.

The invention relates to the field of biological medicine, in particular to application of kaempferol in preparation of a medicine for promoting cilia growth, and provides application of kaempferol or pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof in preparation of the medicine for promoting cilia growth. And a pharmaceutical composition or combination comprising a therapeutically effective amount of kaempferol or a pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof.

The invention provides a rabbit polyclonal antibody aiming at primary cilia and a preparation method thereof, and belongs to the technical field of biology, and the preparation method comprises the following steps: taking a 200-428 amino acid sequence of human ARL13B protein as a target fragment, synthesizing a gene after codon optimization, cloning the gene to a pet-32a expression vector, and carrying out induced expression in escherichia coli to obtain the rabbit polyclonal antibody aiming at primary cilia. Purifying to obtain high-purity immunogen protein; the nucleotide sequence of the synthetic gene is as shown in SEQ ID NO.1, and the amino acid sequence of the high-purity immunogen protein is as shown in SEQ ID NO.2. Immunizing a New Zealand white rabbit with the high-purity immunogen protein to obtain immune serum; and purifying the immune serum to obtain the rabbit polyclonal antibody. The prepared rabbit polyclonal antibody has higher specificity and signal strength, the titer can reach 1: 64000 or above through ELISA detection, primary cilia can be marked more clearly in a paraffin section immunofluorescence experiment, background noise is remarkably reduced, and the reliability of an experiment result is improved.

To provide a method for preparing a respiratory organoid by proliferating and differentiating respiratory epithelial cells.SOLUTION: Unlike conventional respiratory organoids in which epithelial cells existing in an apical region of a respiratory organ are generated on an interior of the organoid, the respiratory organoid of the present disclosure is shown in a form in which ciliated structures, which are epithelial cells, are located in an outer layer of the organoid, secretory cells are present on the interior, and basal stem cells existing at a base of respiratory tissue are also present on the interior of the organoid. It has a form similar to an actual respiratory tissue, and it was observed to exhibit normal functions of the epithelial cells, such as motility of ciliated cells. Therefore, when an organoid is prepared using the preparation method of the present disclosure, a respiratory organoid that can well mimic the actual tissue can be prepared.SELECTED DRAWING: Figure 1

The invention relates to the field of biological medicine, in particular to application of kaempferol in preparation of a medicine for promoting cilia growth, and provides application of kaempferol or pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof in preparation of the medicine for promoting cilia growth. And a pharmaceutical composition or combination comprising a therapeutically effective amount of kaempferol or a pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof.

Disclosed are methods and systems for modifying the motility of engineered multicellular organisms. The engineered multicellular organisms include an aggregate of cells, e.g., ciliated cells. The methods may include exposing the engineered multicellular organism to an acoustic stimulation, e.g., sound at a frequency of about 5 Hz to about 1000 Hz.

The present invention relates to the field of treat age-related cognitive declines. In this study, the inventors report that GPR158, which localizes at the PC, is required for OCN- dependent autophagy induction in hippocampal neurons. Consistently, the mobilization of key autophagy players by OCN in hippocampal neurons requires core PC-proteins. In aged hippocampi, neuronal PC present an abnormal morphology, correlated with a reduction of the major core ciliary proteins levels. Conversely, restoration of their levels in aged hippocampi is sufficient to improve autophagy and cognitive deficits. Lastly, they show that core PC-proteins are required to integrate the rejuvenating effects of OCN in the hippocampus, thereby ameliorating age-related cognitive deficits. Altogether, these findings demonstrate a new paradigm for the neuron / systemic milieu communication and significantly advance our understanding of the regulatory mechanism mediating the rejuvenating effects of the pro-youth blood factor, OCN, in hippocampal neurons. This study also provides the foundation for novel therapeutic strategies to treat age-related cognitive decline, as well as neurological deficits in ciliopathies. Thus, the present invention relates to an activator of the primary cilia (PC) for use in the restoration and / or improvement of cognitive functions in a subject in need thereof.

The present application provides an inhibitor of primary cilium of an immune-related cell and an application thereof. The solution is to use an inhibitor of primary cilium of an immune-related cell containing an ERK1 / 2 inhibitor as an effective component.

The invention provides a Matrigel-free everted cilia organ and a culture method and application thereof.According to the method, a serum-free BPE-free amplification system, a Matrigel-free everted cilia organ and a high-throughput compatible culture method and an everted cilia differential medium are adopted, a culture system with definite chemical components is adopted for non-invasive sampling of a nasal swab, matrigel is not needed, and the Matrigel-free everted cilia organ is obtained. According to the method, the culture mode that the 96U-shaped bottom ultra-low adsorption pore plate / Agrrewell pore plate / cell ball honeycomb sheet pore plate is adopted for 3D differentiation culture to form the organoid is adopted, the purposes of completing amplification and cilia differentiation in a low-cost, standardized and high-throughput mode are achieved, and a feasible path is provided for subsequent large-scale drug screening and individualized precise diagnosis and treatment.

An oleanane-type triterpenoid or a glycoside thereof is useful as a compound having an effect of inducing formation of cilium and activating hair papilla cells or hair matrix cells.

The invention discloses nanoparticles and an electromagnetic transfection system for repairing cartilage injury as well as a preparation method and application of the nanoparticles and the electromagnetic transfection system, and belongs to the technical field of biological medicines. The preparation method of the nanoparticles comprises the following steps: S1, carrying out amination modification on SPIONs coated with silicon dioxide, so as to obtain aminated SPIONs; and S2, covalently binding small interfering RNA to the surface of the aminated SPIONs obtained in the S1 through a cross-linking agent, thereby obtaining the product. The si-SIRT3 (at) SPIONs nano delivery system is successfully constructed, non-invasive EMF exposure is combined, the problem that the cartilage tissue transfection efficiency is low is solved, SIRT3 overexpression, primary cilia injury and cartilage matrix degradation caused by factors such as T-2 toxin can be effectively reversed, the obvious cartilage protection and repair effects are shown in vitro and in vivo, and the cartilage tissue transfection system has the advantages of being high in biocompatibility, good in biocompatibility and good in biocompatibility. A novel targeted therapy normal form is provided for treating cartilage injury diseases such as Kaschin-Beck disease.

The present invention provides methods and kits for detecting cilium markers in samples and uses thereof for detecting and treating endothelial damage or dysfunction or vascular injury in the subject. Also provided are methods of sample processing, methods for detecting cilium markers on platelets, and methods of detecting traumatic brain injury.

The application belongs to the technical field of acoustic wave detection, and specifically discloses a cilium type acoustic wave detection device and method. The detection device comprises a substrate, a bionic cilium array and a vibration detection module. The bionic cilium array is composed of bionic cilia with different lengths and diameters simulating the structure of cochlear hair cells. The substrate is in close contact with the bottom of the bionic cilium and is connected. The bionic cilium array and the substrate are prepared by 3D printing, reverse mold method or MEMS processing method. The application adopts the above-mentioned cilium type acoustic wave detection device and method, directly analyzes the frequency of the acoustic wave by using the bionics principle and micro-processing technologies such as 3D printing, solves the problems of strong dependence on power supply, complex structure, high algorithm requirement and the like in the prior art, realizes the mechanical resonance characteristics of the bionic cilium array with different length-diameter ratios in response to a specific frequency acoustic wave, and realizes low power consumption, high sensitivity and high selectivity of acoustic wave detection.

Provided is a method for producing a cell population that includes pseudostratified ciliated respiratory epithelial cells, the method including culturing, in a medium that flows in a particular direction, a cell population that includes at least one of respiratory epithelial precursor cells and respiratory epithelial tissue stem cells. Also provided is a cell population obtained by said production method. Provided is a method for inducing differentiation of respiratory epithelial precursor cells and respiratory epithelial tissue stem cells into pseudostratified ciliated respiratory epithelial cells, the method including culturing, in a medium that flows in a particular direction, a cell population that includes at least one of respiratory epithelial precursor cells and respiratory epithelial tissue stem cells. Also provided is a cell population that includes pseudostratified ciliated respiratory epithelial cells that have been differentiated from pluripotent stem cells, endodermal precursor cells, respiratory epithelium precursor cells, or respiratory epithelium tissue stem cells, and that demonstrates well-coordinated ciliary movement across cells.

The application provides ciliate-specific primers, ciliate-specific long primers and kits suitable for amplicon sequencing technology and applications, and specifically belongs to the field of molecular biology detection and high-throughput gene sequencing technology.The ciliate-specific primers include a forward primer CS322F and a reverse primer CS819R;the ciliate-specific long primers suitable for amplicon sequencing include a forward long primer LCS322F and a reverse long primer LCS819R.The ciliate-specific primers have broad-spectrum generality for ciliophora, are suitable for a short read length high-throughput sequencing method, and can be used for comprehensively analyzing the diversity and ecological functions of a ciliate community.The application can realize accurate detection and efficient analysis of the ciliate community, and provides technical support for environmental monitoring, germplasm resource development and ecological assessment.

The present application relates to a mammal fallopian tube organoid within a cell-culture insert comprising a porous membrane supported by a frame, the cell-culture insert being accommodatable within a culture vessel to separate the culture vessel into two compartments providing an upper compartment and a lower compartment, and wherein the mammal fallopian tube organoid comprises at least ciliary cells and secretory cells, as well as uses and methods thereof.

Disclosed are methods of using multicellular constructs in methods to detect anti-aging or pro-regenerative compositions. The disclosed organisms comprise an aggregate of ciliated cells, and the organisms move when the ciliated cells are actuated. Also disclosed are populations of motile multicellular constructs with improved longevity, methods of generating motile multicellular constructs with improved longevity, and methods of reducing the cellular age of a sample of ciliated epithelial cells.

The invention relates to a spring ring assembly and a preparation method. The spring ring assembly comprises a spring ring body, a core wire and cilium bundles. The core wire penetrates through the spring ring body. The cilium bundle is arranged on the spring ring body and comprises a plurality of cilium bodies made of degradable high polymer materials. The cilium bundles are arranged to be in a bundle shape when the spring ring assembly is conveyed through the micro catheter and are in a radial shape when the spring ring assembly is released into the tumor sac. The cilium bundles are made of degradable high polymer materials and can be gradually degraded in vivo, so that inflammation stimulation caused by long-term retention of foreign matters is effectively avoided; meanwhile, the cilia bundle is kept in a gathering state in the conveying process, the friction resistance between the cilia bundle and the micro catheter is reduced, it is guaranteed that the spring ring assembly is smoothly and accurately conveyed to the target focus position, the cilia bundle is automatically unfolded to be of a radial structure after being released to a tumor cavity, the effective contact area between the cilia bundle and the tumor cavity is increased, and the physical blocking effect on blood flow is enhanced; and rapid formation of local thrombus is promoted.

The invention relates to the skipping of the CEP290 exon 36 in an individual suffering from a retinal dystrophy accounted for by a nonsense mutation or a premature termination codon generated by a frameshift mutation in exon 36 or an upstream exon, including the c.4723A>T, c.4771C>T, c.4714G>T, c.4786_4790del, c.4791_4794del, c.4732G>T, c.4625_4626insCATG (35), c.4792_4795del, c.4801C>T, c.4805C>T, or c.4811G>A mutations, to bypass protein truncation and lessen retinal damages. Here, studying fibroblasts from control individuals, and two patients carrying the CEP290 c.4723A>T nonsense mutation, they show low levels of spontaneous skipping of exon 36 arising from both endogenous basal skipping and mutation-induced skipping. The minimally shortened and mutation-free CEP290 mRNA produced by skipping of exon 36 in the fibroblasts of the two patients is translated into a protein isoform that localizes at the centrosome and allows the formation of primary cilia, yet with elongated axonemes. Using an AON consisting of a sequence set forth as SEQ ID NO: 1, complementary to a nucleic acid sequence of CEP290 pre-mRNA, wherein said AON targeting an mRNA encoding the donor splice site (H36D) is capable to alter splicing by blocking the recognition of exon 36 and bypass protein truncation while maintaining the open reading frame, leading to the production of near full-length CEP290 protein, they were able to increase the abundance of the alternatively spliced mRNA and shortened protein and to reduce axonemal length in patient cells.

The invention provides a construction method and application of a bifluorescence reporter gene animal model for tracking transdifferentiation from airway cilia cells to mucous cells. A transgenic animal model capable of specifically and permanently marking and tracking airway ciliary cell fate transformation is constructed by utilizing a Cre-LoxP gene recombinase system and combining with double promoters and double fluorescence reporter genes. In the progeny, normal cilia cells only express GFP (green). When the cell starts to express MUC5AC under external stimulation (such as PM2.5 and allergen), the existing Cre recombinase in the cell nucleus can recognize and cut off the loxP-Stop-loxP sequence at the downstream of the MUC5AC gene, so that the expression of the RFP gene is started. Finally, mucus-cilia cells obtained by transdifferentiation of cilia cells can simultaneously express GFP (green fluorescent protein) and RFP (yellow), or only express RFP (red) after completely losing cilia cell characteristics. The irreversible color conversion provides a definite evidence for the'provenance and today 'of the cells.

Disclosed is a method for treating ciliopathies-related diseases, comprising administering to a subject in need thereof an effective amount of a compound that targets at least one G protein-coupled receptor.Provided is a method for identifying therapeutic agents for treating diseases with ciliopathies, comprising providing an animal model system of ciliopathies to test putative therapeutic agents, administering a disrupting agent to the animal, treating the administered animal with the putative therapeutic agent, comparing the measurable phenotype of the treated animal with that of untreated animals, and identifying the therapeutic target as a treatment for ciliopathies if the measurable phenotype of the treated animal is reduced compared to that of the untreated animal. [Selection diagram] Figure 32