123 results about "Cilium" patented technology

The present invention relates to certain novel dinucleotides and formulations thereof which are highly selective agonists of the P2Y2 and / or P2Y4 purinergic receptor. They are useful in the treatment of chronic obstructive pulmonary diseases such as chronic bronchitis, PCD, cystic fibrosis, as well as prevention of pneumonia due to immobility. Furthermore, because of their general ability to clear retained mucus secretions and stimulate ciliary beat frequency, the compounds of the present invention are also useful in the treatment of sinusitis, otitis media and nasolacrimal duct obstruction. They are also useful for treatment of dry eye disease and retinal detachment as well as facilitating sputum induction and expectoration.

The present invention relates to methods and compositions for treating diseases ameliorated by increased mucociliary clearance and mucosal hydration by administering an effective amount of a sodium channel blocker as defined herein and an osmolyte to a subject to a subject in need of increased mucociliary clearance and mucosal hydration.

A method of facilitating the obtaining of a mucus sample from at least one lung of a subject comprises administering to at least one lung of the subject, in an amount effective to hydrate lung mucous secretions and / or stimulate cilia beat frequency therein, uridine 5'-triphosphate, an active analog thereof, or a pharmaceutically acceptable salt of either thereof, and, optionally, concurrently administering to said at least one lung a physiologically acceptable salt in an amount effective to hydrate lung mucus secretions therein. A sputum or mucus sample is then collected from said at least one lung, which sample can then be analyzed for lung disease. Pharmaceutical compositions useful for carrying out the method comprise UTP or a salt thereof, alone or in combination with a physiologically acceptable salt, or a pharmaceutically acceptable salt of either thereof. The composition may be a liquid / liquid suspension composition or a dry powder composition.

The invention relates to a sewage double-biomembrane deep treatment technology comprising the steps: sewage enters an anaerobic tank, an anoxic tank and an oxygen maintaining tank after being pretreated by a grid and settled sand, and mud releases phosphorus in the anaerobic tank; the sewage is denitrified in a denitrification mode in the anoxic tank so as to remove most of organic matters; cilium biological fillers are arranged in the oxygen maintaining tank, biomembranes are attached to the cilium, absorb the phosphorus in excess and degrade the partial organic matters; the sewage enters a secondary sedimentation tank to carry out sedimentation treatment through the oxygen maintaining tank and then enters an aeration biological filter tank; granule biological fillers are arranged in the aeration biological filter tank, and the biomembranes are attached to granules, degrade the residual organic matters in the sewage and convert ammonia nitrogen into nitrate nitrogen; one part of the discharged water of the aeration biological filter tank flows back into the anoxic tank, and the other part is discharged; one part of the mud generated by the secondary sedimentation tank back flows into the anoxic tank, and the other part of the mud is discharged out of a system. The sewage double-biomembrane deep treatment technology has the advantages of investment saving, low operation cost, little land occupation and good treatment effect.

The invention discloses a microstructure of a four-cilium bionic MEMS vector acoustic sensor. The microstructure comprises a square frame and a #-shaped suspension beam arm erected at the centre of the square frame, whereon each section of the beam of the #-shaped suspension beam arm is composed of a wide section and narrow sections which are symmetrically arranged at the both sides of the wide section; piezoresistors with the same resistance are arranged on the narrow sections respectively; the piezoresistors form two groups of Wheatstone full bridges; the two groups of Wheatstone full bridges are used for measuring aquatic signals in two mutually vertical directions respectively; vertical cilia are fixedly arranged on each square connection body. According to the microstructure disclosed by the invention, four bionic cilia are integrated on one chip by virtue of an MEMS technology, and the #-shaped suspension beam arm structure is adopted, and local stress concentration is improved in the narrow-wide-narrow form of each section of the beam, thus improving the resistance changes of the piezoresistors, and then improving the sensitivity of the structure; therefore, the contradiction between the sensitivity and the frequency response range of the existing MEMS bionic hydrophone is solved.

This invention provides methods for transducing a ciliated cell with a recombinant serotype 2 adeno-associated virus (AAV) vector. Additionally, the invention provides methods of treating diseases associated with a mutated gene by transducing a ciliated cell with a recombinant serotype 2 AAV vector containing a corrective transgene.

The invention relates to a nasal cavity cleaning agent applied to health care of the nasal cavity of a human body. The nasal cavity cleaning agent comprises the following components in percentage by weight: 0.9 to 1.2 percent of sea salt, 0.05 to 0.2 percent of buffering agent and the balance of water, wherein the buffering agent is prepared from citric acid and sodium citrate in the weight proportion of 1 to 100. The nasal cavity cleaning agent has the advantages that: the environment in the nasal cavity is maintained because of sodium chloride with the physical concentration and pH value; 2, secretions in the nasal cavity can be diluted and discharged, nose scabs are eliminated, and breath becomes smooth; 3, the nasal cavity is moisturized and bleeding caused by dryness of the nasal cavity is avoided; 4, pathogenic bacteria and harmful substances in the nasal cavity are cleaned and the automatic secreting and cleaning function of the nasal cavity is recovered; 5, nasal mucosa is protected and the ciliated epithelium of the nasal mucosa is promoted to swing; and 6, the nasal cavity cleaning agent does not have any adverse reaction and any side effect on human bodies because no chemical drug is contained in the nasal cavity cleaning agent.

The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.

The ciliated protozoan Tetrahymena exemplifies a recombinant system for the expression of heterologous nucleic acids, preferably on the plasma membrane surface. Integration of a heterlogous nucleic acid into the β-tubulin gene, BTU1, of a paclitaxel-sensitive T. thermophila mutant that possesses btu1-1K350M β-tubulin allele allows screening for transformants using negative selection, as transformants have restored paclitaxel resistance. Transgenic ciliated protozoa of the invention can serve as live vaccines. For example, transgenic Tetrahymena expressing Ichthyophthirius multifiliis i-antigen protein on their surface are effective vehicles for vaccination of freshwater fish against infection by I. multifiliis.

The invention provides a magnetic and force cilium bionic sensor and a preparation method thereof. The sensor is formed by a signal sensitive layer and an electrode layer. The signal sensitive layer is a magnetic conductive cilium array. The preparation method comprises the following steps: 1) placing suspension liquid formed by resin materials and magnetic particles in a magnetic field environment, carrying out heating treatment until solvent is evaporated and resin is cured and obtaining a magnetic cilium array; and 2) coating graphene oxide onto the surface of the obtained magnetic cilium array, and carrying out chemical reduction treatment to obtain the magnetic conductive cilium array. The electrode layer is a flexible macromolecule film layer having an interdigital conductive network structure. The sensor has the advantages of low cost, high sensitivity, good repeatability and having response to magnetic and force signals and the like, and has a good application prospect in the fields of biological health detection, smart screen, human-computer interaction and electronic skin and the like.

The invention relates to a method for preparing and culturing a culture medium for the supravital culture of seawater scuticociliatida ciliates, and relates to the field of the study of aquatic animal diseases. The method for preparing the in-vitro culture medium comprises the following steps of: mixing fresh brain tissue and muscular tissue of paralichthys olivaceus or turbot fish in a mass ratio of (1:1)-(3:1), and homogenizing to form muddy flesh; adding sterilized seawater into the muddy flesh in a mass / volume ratio of the muddy flesh to the seawater of (1:45)-(1:55), and performing waterbath at the temperature of between 65 and 70 DEG C for 2 hours to prepare the in-vitro culture medium, wherein the prepared culture medium is stored at the temperature of 4 DEG C for a long time. Themethod for preparing the culture medium is simple, and complex components are not needed to be added; and the seawater scuticociliatida ciliates are subjected to the supravital culture by utilizing the culture medium prepared by the method, and the scuticociliatida ciliates can survive for over 30 days, so that the foundation is established for the further study of the pathogenesis and preventionand control technology of the scuticociliatida ciliates.

The invention belongs to the department of otolaryngology-head and neck and field of washing and therapeutic apparatus for nasal cavity, which aims at recovering the physiological function of nasal mucosa cilium system. The washing and therapeutic apparatus for nasal cavity comprises a forming system of bionic massage washing stream with adjustable pressure and an intelligent constant temperature system; the core of the main body is controlled by an 892051 single chip microcomputer which also carries out digital control for the temperature of washing stream and for the frequency as well as pressure of the bionic massage washing stream, while the flow rate is controlled and regulated manually. The constant temperature ranges between 35 and 42 DEG C; the frequency of the bionic massage washing stream ranges from 15 to 25 times per second; the pressure of water columns is regulated between 10 and 15 cmH2O(0.98 to 1.47KPa); and the regulation range of the flow rate is fixed between 70 and 100ml per minute. A liquid medicine container and an insulated heating container with heat transfer are combined to be a 'dual-container structure' so as to avoid electric leakage and denaturation and going-bad of the washing liquid medicine after being heated directly. The positive-pressure bionic massage washing stream from the washing and therapeutic apparatus can effectively recover the physiological function of nasal mucosa cilium system and realize the therapy of nasal diseases, thus having wide potential application and high clinical value.

The ciliated protozoan Tetrahymena exemplifies a recombinant system for the expression of heterologous nucleic acids, preferably on the plasma membrane surface. Integration of a heterlogous nucleic acid into the β-tubulin gene, BTU1, of a paclitaxel-sensitive T. thermophila mutant that possesses btu1-IK350M β-tubulin allele allows screening for transformants using negative selection, as transformants have restored paclitaxel resistance. Transgenic ciliated protozoa of the invention can serve as live vaccines. For example, transgenic Tetrahymena expressing Ichthyophthirius multifiliis i-antigen protein on their surface are effective vehicles for vaccination of freshwater fish against infection by I. multifiliis.

This invention discloses a RNA adaptor preventing typhoid typhi from intruding into human body cells. This invention discloses a method for preparing the RNA adaptors by preparing and purifying TVB type cilium structure fusion protein-agarose 4B, constructing single strand DNA library randomly, comining double-strand DNA library, amplifying PCR, preparing RNA libraries, screen SELEX, selecting RCR to get RNA adaptors, which can be the agonist of typhi.

The invention relates to a multiple stress concentrated type MEMS bionic hydrophone, and solves the problems including that a present MEMS bionic hydrophone can only measure vector information of a sound field, is relatively low in flexibility and frequency response bandwidth and is fuzzy in port and starboard. The hydrophone comprises a bottom silicon layer, an oxide layer and a top silicon layer, four tetra-beam cilium type acoustoelectric transduction structures are distributed in the top silicon layer uniformly, the bottom of each tetra-beam cilium type acoustoelectric transduction structure includes a cavity, each of the two ends of each cantilever beam is provided with a settling tank and an internally shrunk gap, the four cavities are distributed uniformly on the oxide layer, the top silicon layer is sputtered with upper electrodes at positions facing the cavities respectively, the bottom silicon layer is sputtered with lower electrodes at positions facing the cavities respectively, and each upper electrode is surrounded by four oil filling holes which are distributed in the top silicon layer uniformly. The hydrophone of the invention is simple in structure, greatly widens the effective frequency band range, provides convenience for installation and test by monolithic integration, improves the installation precision, uses a single sensor to realize a sensor array in small size, and overcomes the problem that different hydrophones in a traditional array are inconsistent.

The invention discloses an artificial-cilia-based simulated-cochlea-semicircular-canal-contained rotation acceleration sensor comprising more than one hollow bending pipeline. Two ends of the pipeline are communicated with a sealed container; and the pipeline and the container are filled with insulating liquid. More than one protruding fiber unit is arranged on a crista ampullaris part inside each pipeline. According to the invention, the sensor is divided into three units distributed in a space coordinate system layout, thereby sensing rotation at different direction. When the overall sensor is rotated, the fiber units at three directions are deformed differently; and because of different electrode positions, charges or voltages generated on the electrodes are different. Therefore, the direction and value of the multi-dimensional rotation accelerated speed can be determined by analyzing polarity and values of three fibers by a charge amplifier and a processing unit.

A device comprises: one or more cantilevered biomimetic cilia, and a liquid disposed among the one or more biomimetic cilia, wherein individual biomimetic cilia are at least partially submerged in the liquid, and wherein the biomimetic cilia are arranged for excitation into resonance, such as for mixing and pumping via the resonant behavior of the excited cilia.

The present invention belongs to the field of medicine preparation, and is nasal in-situ gel of scopolamine with phase change property as one new kind of nasal medicine preparation form. The medicine preparation is prepared with scopolamine or its pharmaceutically acceptable salt or ester derivative, hydrophilic gel and pharmaceutically essential supplementary material. It is water solution of scopolamine containing environment sensitive polymer material, and is liquid at normal state and forms gel on the surface of nasal mucous membrane fast after nasal cavity administration. It has delayed medicine elimination, raised bioavailability, no preparation and use inconvenience like that of common high viscosity gel preparation and no toxicity on nasal cilium.

The invention relates to the technical field of biology and discloses a colonization probiotic preparation and application and medicines thereof. The colonization probiotic preparation is used for directly acting on the respiratory mucosa and comprises a surfactant and probiotics. By the surfactant, modification of a sol layer and a gel layer of respiratory tract mucus is realized, probability and speed in contact of the probiotics with respiratory mucosa cells are increased, cell surface hydrophobicity of the probiotics is changed, an adhesion rate of the probiotics and respiratory mucosa epithelial cells is increased, and accordingly the technical bottleneck of difficulty in colonization of the probiotics to the respiratory mucosa under expelling action of a mucociliary transmission system is solved. The colonization probiotic preparation can be applied to fields of preparation of medicines for treatment of rhinitis, treatment of asthma, treatment of allergic dermatitis or treatment of cranial nerve diseases, anti-nasopharynx-cancer, anti-lung-cancer or anti-cold medicines or medicines for prevention or treatment of respiratory infectious diseases.

The invention discloses a dynein mosaic type recombinant human type-B adenovirus and a preparation method thereof. The skeleton of the dynein mosaic type recombinant human type-B adenovirus is a human type-B adenovirus genome, and a base sequence which encodes a receptor binding domain of dynein is a base sequence which encodes a corresponding domain of a human type-C adenovirus. By a molecular cloning method, Ad5-knob gene fragments are cloned and replaced to recombinant shuttle plasmids, in-vitro recombinant on the Ad5-knob gene fragments and a recombinant human type-3 adenovirus genome is realized, obtained knob gene fragments are replaced into type-5 recombinant human type-3 adenovirus genome, and therefore, dynein mosaic type recombinant human type-3 adenovirus rAd3-FK5 is obtained. The dynein mosaic type recombinant human type-3 adenovirus rAd3-FK5 can be infected with mouse primitive epithelial cells and golden hamster lung and kidney primitive cells in vitro, and the infection efficiency of the dynein mosaic type recombinant human type-3 adenovirus rAd3-FK5 is close to that of Ad5, and is much higher than that of a parent strain rAd3E, in golden hamster cells, significant copying exists, and the dynein mosaic type recombinant human type-B adenovirus can be used for small animal model research of human type-3 adenovirus vaccines and antiviral drug evaluation.

This invention relates to cerebrospinal fluid shunts, and a method of treating shunt catheters, whereby the interior lumen of a shunt catheter, comprised of a biocompatible matrix, is seeded with cells for placement within cerebrospinal fluid pathways of the central nervous system. The seeded cells have at least one of the following characteristics: (1) they are of a polarized ependymal epithelial phenotype with tight junctional complexes and apical cilia directed toward the lumen of the catheter; (2) they maintain stem / progenitor characteristics and are capable of neurogenesis; (3) they maintain stem / progenitor characteristics and are capable of gliogenesis.

Provided is a method of fabricating magnetic cilia including the following steps. Step (A): A mold is provided in which a plurality of micro-channels are formed, wherein the aperture of each of the micro-channels is between 50 μm and 350 μm, and the depth of each of the micro-channels is between 500 μm and 3,500 μm. Step (B): A raw material is spread onto the mold and filled into each of the micro-channels, wherein the raw material includes a polymer and magnetic particles dispersed therein. Step (C): A heat treatment is performed to harden the raw material in each of the micro-channels into a magnetic cilium. Step (D): A mold release process is performed to isolate each of the magnetic cilia from each of the micro-channels.

The invention discloses a staining method of ciliates, belongs to the technical field of biology, and provides a staining method of ciliates to solve the problems that a traditional ciliate staining method is long in staining time and fussy in staining step, and cytostome and cilium cannot be clearly displayed. A saturated ethyl alcohol mercuric chloride solution is adopted as a fixing agent to carry out pre-fixation on ciliate bodies. In addition, before the pre-fixation operation, to-be-produced ciliate bodies are attached to a to-be-dyed glass slide; a plurality of steps such as dropwise adding protein glycerinum water and collodion are omitted. The staining method disclosed by the invention is convenient to operate; the sensitivity and the staining effect of silver staining of the ciliate protein are greatly improved; and rapid, efficient and accurate display of infraciliature in morphological classification and cytological studies of the ciliates is achieved.

The invention discloses a production method for fermenting wine by using walnut green husks and pine needles, which comprises the following steps of: harvesting walnut fruits, sterilizing and washing the obtained green husk raw materials, pulping the walnut green husks, removing cilium from the pine needles and then grinding the pine needles into pulp; mixing the walnut green husk juice and the pine needle pulp according to a ratio, heating the mixed solution and filtering with a screen to obtain juice; adjusting ingredients, adding white granulated sugar and citric acid and then re-adjusting acidity; cooling and adding activated wine dried yeast in an amount based on the weight of the juice into a fermentation tank, performing primary fermentation and obtaining raw material liquid; turning the tank upside down to separate dregs, performing post-fermentation, exchanging buckets, aging and then storing the obtained wine; aging for 30 days, filtering with a filter, filling and sterilizing the filtrate to obtain the finished product. The alcoholic beverage is prepared by the walnut green husks and the pine needles, so that the comprehensive recycling of the walnut green husks and the pine needles is realized, energy is saved and environment is protected; and pharmaceutical effects of the alcoholic beverage required by a human body are achieved by using the beneficial ingredients of the walnut green husks and the pine needles, so that the effects of strengthening the body and caring health are achieved.

The invention provides an agent fed via nasal to treat the emesis caused by cancer chemotherapy, radiotheraphy or surgery. The inventive agent comprises palosnon drug, or medical accept salt, and medical effective findings. The inventive agent fed via nasal can effectively be absorbed by schneiderian membrane to restrain emesis, without simulation on schneiderian membrane and little cilium toxicity.

The invention relates to the field of medical nursing, in particular to a nasal cavity mucosa cilium nursing flushing fluid and application thereof. The flushing fluid comprises acetylcysteine, a pharmaceutical salt of acetylcysteine or a pharmaceutical precursor of acetylcysteine, and has the function of nursing the nasal cavity mucosa cilium. The flushing fluid has the effects of cleaning the nasal cavity mucosa cilium, promoting the regeneration capacity of the cilium cells of rhinitis patients, avoiding swing of the mucus cilium of the rhinitis patients, increasing the, shortening the cleaning rate recovery cycle of the rhinitis patients, regulating the swing frequency of the nasal cavity mucosa cilium, and the like.

The invention discloses a gene chip for screening a primary cilium-related disease causative gene. The gene chip comprises hundreds of probes. The probes are used for detecting a primary cilium-related disease related gene. The gene chip realizes purposeful gene screening on primary cilium-related disease clinical phenotype cases, can fast and effective identify causative genes, and provides basis for primary cilium-related disease early diagnosis and individual treatment.

Compositions and methods are provided for identifying agents which have efficacy for the treatment of disorders related to aberrant cilial structure and function, including polycystic kidney disease.