41 results about "Cilium" patented technology

The present disclosure relates to a method and an apparatus for measuring motility of ciliated cells in a respiratory tract. The method includes the operations of: acquiring image data including a plurality of frames of respiratory tract organoids; identifying positions of ciliated cells by performing motion-contrast imaging on the image data; when a region of interest (ROI) related to the position of the ciliated cells is selected, measuring a ciliary beat frequency (CBF) related to motility of cilia included in the selected region of interest using cross-correlation between the plurality of frames; and expressing the cilia included in the region of interest in a preset display method on the basis of the range of the measured ciliary beat frequency.

The invention relates to the technical field of sensors, in particular to a bionic hair tactile sensor and application, and the sensor comprises a substrate and a bionic cilia array arranged on the substrate; the bionic cilia array comprises a plurality of flexible bionic cilia arranged in an array, and a marking layer is arranged at the bottom of the flexible bionic cilia; an optical system is integrated in the substrate, the optical system comprises an imaging module and a micro prism, and the micro prism reflects the deformation of the marking layer at the bottom of the flexible bionic cilia to the imaging module; and the imaging module is used for capturing deformation data of the marking layer and sending the deformation data to the deep learning algorithm module to realize decoupling and resolution of three-dimensional force. According to the invention, high-resolution multi-dimensional force detection and direction detection are realized through mark point change caused by cilium deformation, and the array design is expanded to a sliding sensing function and a multi-modal object identification function.

The invention discloses an analysis method of a cilia magnetic sensor, and relates to the technical field of sensors, and the analysis method comprises the following steps: carrying out a tensile experiment on cilia with different magnetic powder contents in a selected scene to obtain Young modulus of the cilia with different magnetic powder contents in the selected scene; obtaining the optimal magnetic powder content of the cilia in the selected scene based on the Young modulus of the cilia with different magnetic powder contents in the selected scene; the optimal cilium height, the optimal cilium diameter and the optimal cilium spacing of the cilium in the selected scene are obtained through finite element simulation analysis; performing a normal pressure sensing experiment on the cilia magnetic sensor in the selected scene, performing a sliding sensing experiment on the cilia magnetic sensor in the selected scene, and performing a non-contact sensing experiment on the cilia magnetic sensor in the selected scene; according to the invention, the design and application scenarios of the cilium magnetic sensor can be optimized.

The invention relates to the technical field of biology, and particularly provides an endometrial organ culture method. By optimizing the endometrial organoid differentiation culture system, compared with an existing culture mode, the optimized culture system is easy to operate, the organoid with more complete physiological functions can be prepared without co-culture with stromal cells, continuous culture can be conducted for at least one year or above, and the obtained organoid is complete in secretion function and free of secondary pollution. The phenotype of cilia cells is more obvious.

The present disclosure relates to a method for preparing a respiratory organoid by proliferating and differentiating respiratory epithelial cells. Unlike the conventional respiratory organoids in which epithelial cells existing in the apical region of the respiratory organ are generated on the interior of the organoid, the respiratory organoid of the present disclosure is shown in a form in which ciliated structures, which are epithelial cells, are located in the outer layer of the organoid, secretory cells are present on the interior, and basal stem cells existing at the base of respiratory tissue are also present on the interior of the organoid. It has a form similar to an actual respiratory tissue, and it was observed that the epithelial cells exhibited normal function, such as motility of ciliated cells. Therefore, when an organoid is prepared using the preparation method of the present disclosure, a respiratory organoid that can well mimic actual tissue can be prepared.

The invention relates to the technical field of thrombus mining robots, in particular to a micron robot with an asymmetric cilia structure, a preparation method and application. The micro-robot is of an asymmetric composite structure and is composed of a smooth hemisphere and a cilia-shaped structure, sandwich type layered arrangement of a PLGE cilia-shaped nano structure, an MNPs interlayer and a PS sphere core is formed on the surface and the interior of the micro-robot from outside to inside, and cooperative regulation and control of morphology and functions are achieved by regulating and controlling the material proportion of PS and PLGE. The thrombus mining robot shows an efficient thrombolysis effect in a plurality of in-vitro and in-vivo models, and rapid fibrinolysis, deep thrombus penetration and efficient mining of single red blood cells are realized, so that the limitation of a traditional thrombolysis agent is overcome. In addition, the thrombus digging robot has excellent biocompatibility and biological safety, has no obvious cytotoxicity or damage to main organs, and supports the safety of the thrombus digging robot in clinical application.

The invention belongs to the technical field of bionic cilium sensors, and particularly discloses a magnetic bionic cilium and vesicle complex and a preparation method and application thereof.The preparation method comprises the following steps that S1, a cilium array male mold is prepared; then carrying out oxygen plasma treatment, silanization and curing stabilization; s3, injecting the magnetic composite precursor into a polydimethylsiloxane female die, applying pressure, and striking off; and S4, coating the mold surface with an Ecoflex precursor, performing thermocuring forming, performing magnetization treatment, and performing stripping to obtain the magnetic bionic cilia. According to the magnetic bionic cilia, the vesicle complex and the preparation method and application of the magnetic bionic cilia, the vesicle complex and the preparation method and application of the magnetic bionic cilia, high-magnetic signal output is achieved while the deformability of the cilia is guaranteed, the magnetic bionic cilia is used for non-contact real-time hemodynamic monitoring, meanwhile, specific response release of the vesicle complex is achieved, and a new thought is provided for design of responsive vascular implants.

The invention discloses a therapeutic target CD40 related to multiple myeloma bone diseases and application of a monoclonal antibody of the therapeutic target CD40. On one hand, it is found that formation and osteogenic differentiation of primary cilia of BMSCs can be effectively recovered by adding a CD40 neutralizing antibody into an MM-BMSCs co-culture system; on the other hand, the CD40 neutralizing antibody is found to reduce bone destruction and tumor load in a Vk * MYC myeloma mouse model. CD40 is expected to become a potential target spot for treating multiple myeloma bone diseases and has a very wide application prospect in the field of medical research.

The present invention relates to the technical field of biomedical materials, and particularly to a solid-liquid biphasic embolic agent with a surface cilia structure and a preparation method thereof; it includes a liquid embolic material in the oil phase and Janus cilia microspheres with hydrophilic cilia structures; the Janus cilia microspheres encapsulate the liquid embolic material in the oil phase, and when encapsulating, the hydrophilic cilia structures in the Janus cilia microspheres are exposed outward in the aqueous phase, so that the surface of the solid-liquid biphasic embolic agent has a cilia structure; the cilia structure can improve the interaction force between embolic material particles and between embolic material particles and the blood vessel wall, has excellent viscoelasticity and deformation ability, can reach finer arterial blood vessels, realize efficient embolization of thick and thin arteries, and improve the efficiency of tumor embolization chemotherapy; and the cilia structure is combined with the solid-liquid biphasic system, which not only improves the embolization effect, but also enhances the stability of the embolic agent, prolongs the action time, and optimizes the biocompatibility, and will have broad application prospects.

The invention relates to a postoperative anti-regurgitation self-cleaning drainage tube which is suitable for chest drainage after pulmonary lobectomy. In order to solve the problems that a traditional drainage tube depends on gravity to conduct passive drainage, a tube cavity is prone to forming a biological membrane due to sediment to cause blockage and drainage efficiency is insufficient, a plurality of cilia are arranged on the inner wall of a drainage tube body in a surrounding mode in the circumferential direction, the sizes of the cilia are gradually reduced from the root to the top end, and the elasticity modulus is reduced in a gradient mode in the drainage direction; and the vibration device is matched to drive the cilia to swing directionally in the same direction as the drainage direction. According to the design, by simulating the respiratory tract cilia function and utilizing the synergistic effect of cilia structural characteristics and vibration excitation, tube wall sediments are actively and physically stripped, a biological membrane is destroyed to form a foundation, and meanwhile, cilia directionally swings to form drainage auxiliary thrust. According to the application, the occurrence rate of lumen blockage can be obviously reduced, the drainage efficiency is improved, the postoperative pain and medical cost of a patient are reduced, the recovery period is shortened, and the clinical efficient drainage and anti-blockage requirements are met.

The present invention relates to a method for producing respiratory organoids by proliferating and differentiating respiratory epithelial cells. [Solution] Unlike conventional respiratory organoids in which epithelial cells present at the apical end of the respiratory tract develop inside the organoid, the respiratory organoid of the present invention has a morphology in which the ciliated structure, which is made up of epithelial cells, is located in the outer layer of the organoid, secretory cells are located inside, and stem cell-like basal cells present at the base of the respiratory tissue are also located inside the organoid. This morphology is similar to that of actual respiratory tissue and has been observed to exhibit normal functions of epithelial cells, such as the motility of ciliated cells. Therefore, by manufacturing organoids using the manufacturing method of the present invention, it is possible to manufacture respiratory organoids that can accurately mimic actual tissue.

The invention relates to application of PDCD5 in preparation of a diagnostic agent or a therapeutic agent for asthenospermia. The invention also discloses a method for constructing an asthenospermia mouse model or inhibiting fibroblast cilia generation by knocking out or knocking down the PDCD5. The application shows that PDCD5 participates in sperm generation, and asthenozoospermia can be caused if the PDCD5 has function deletion mutation in testis. Therefore, the gene locus can be used as a target spot for asthenospermia diagnosis and medication guidance. According to the method disclosed by the invention, the dysfunction of the site is supplemented by the PDCD5 RNA wrapped by the testis injection liposome, so that the asthenospermia caused by the mutation is rescued, the sperm quantity is increased, the sperm malformation rate is reduced, and a new path is provided for the treatment of the asthenospermia.

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

The invention discloses a flexible magnetic response cilia array with mechanical robustness and a preparation method and application thereof. The flexible magnetic response cilia array comprises an array substrate and flexible magnetic response cilia, the array substrate comprises a substrate body and an array structure vertically arranged on the substrate body. The array structure is composed of a plurality of supporting units. The flexible magnetic response cilia vertically grow on the side walls of the supporting units and are parallel to the substrate. The invention provides a flexible magnetic response cilia array with mechanical robustness. The flexible magnetic response cilia are horizontally arranged on the side wall of the supporting unit with the mechanical strength, the cilia are hidden and protected and are not prone to being exposed to an external mechanical damage source, and the friction resistance and the abrasion resistance are improved. When the magnetic field is not applied, cilia are protected; when a vertical magnetic field is applied, the cilia can stand up and exceed the supporting units, and surface property regulation and control such as wettability regulation, liquid drop impact dynamic regulation and control and micro-object control are achieved.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a Gli activator in preparation of a product for treating fiberosity. The invention provides an application of a Gli activator in preparation of a product for preventing and / or treating fiber hair disease. The Gli activator comprises an overexpressed Gli adenovirus, an overexpressed Gli adeno-associated virus, a nucleic acid molecule for improving Gli expression and a small molecule compound for activating Gli. The application accidentally finds that the Gli2 adenovirus can remarkably correct the skeleton phenotype of a mouse model of JATD caused by IFT88 deletion, and the activator with the Gli transcription factor as the target spot can promote the expression of a proliferation related gene c-MYC, or n-MYC, or Cyclin D1; the expression of the apoptosis related gene Bcl2 is inhibited; therefore, bone and cartilage cell proliferation is promoted; bone and cartilage cell apoptosis is reduced; and the effect of activating bone and cartilage cells is achieved, so that the fibrinosis is prevented and / or treated, and a potential target and a new treatment scheme are provided for clinical treatment of generalized fibrinosis.

The invention relates to the field of biological medicine, in particular to application of kaempferol in preparation of a medicine for promoting cilia growth, and provides application of kaempferol or pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof in preparation of the medicine for promoting cilia growth. And a pharmaceutical composition or combination comprising a therapeutically effective amount of kaempferol or a pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof.

The invention provides a rabbit polyclonal antibody aiming at primary cilia and a preparation method thereof, and belongs to the technical field of biology, and the preparation method comprises the following steps: taking a 200-428 amino acid sequence of human ARL13B protein as a target fragment, synthesizing a gene after codon optimization, cloning the gene to a pet-32a expression vector, and carrying out induced expression in escherichia coli to obtain the rabbit polyclonal antibody aiming at primary cilia. Purifying to obtain high-purity immunogen protein; the nucleotide sequence of the synthetic gene is as shown in SEQ ID NO.1, and the amino acid sequence of the high-purity immunogen protein is as shown in SEQ ID NO.2. Immunizing a New Zealand white rabbit with the high-purity immunogen protein to obtain immune serum; and purifying the immune serum to obtain the rabbit polyclonal antibody. The prepared rabbit polyclonal antibody has higher specificity and signal strength, the titer can reach 1: 64000 or above through ELISA detection, primary cilia can be marked more clearly in a paraffin section immunofluorescence experiment, background noise is remarkably reduced, and the reliability of an experiment result is improved.

To provide a method for preparing a respiratory organoid by proliferating and differentiating respiratory epithelial cells.SOLUTION: Unlike conventional respiratory organoids in which epithelial cells existing in an apical region of a respiratory organ are generated on an interior of the organoid, the respiratory organoid of the present disclosure is shown in a form in which ciliated structures, which are epithelial cells, are located in an outer layer of the organoid, secretory cells are present on the interior, and basal stem cells existing at a base of respiratory tissue are also present on the interior of the organoid. It has a form similar to an actual respiratory tissue, and it was observed to exhibit normal functions of the epithelial cells, such as motility of ciliated cells. Therefore, when an organoid is prepared using the preparation method of the present disclosure, a respiratory organoid that can well mimic the actual tissue can be prepared.SELECTED DRAWING: Figure 1

The invention relates to the field of biological medicine, in particular to application of kaempferol in preparation of a medicine for promoting cilia growth, and provides application of kaempferol or pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof in preparation of the medicine for promoting cilia growth. And a pharmaceutical composition or combination comprising a therapeutically effective amount of kaempferol or a pharmaceutically acceptable salt, stereoisomer, solvate, prodrug or derivative thereof.

Disclosed are methods and systems for modifying the motility of engineered multicellular organisms. The engineered multicellular organisms include an aggregate of cells, e.g., ciliated cells. The methods may include exposing the engineered multicellular organism to an acoustic stimulation, e.g., sound at a frequency of about 5 Hz to about 1000 Hz.

The present invention relates to the field of treat age-related cognitive declines. In this study, the inventors report that GPR158, which localizes at the PC, is required for OCN- dependent autophagy induction in hippocampal neurons. Consistently, the mobilization of key autophagy players by OCN in hippocampal neurons requires core PC-proteins. In aged hippocampi, neuronal PC present an abnormal morphology, correlated with a reduction of the major core ciliary proteins levels. Conversely, restoration of their levels in aged hippocampi is sufficient to improve autophagy and cognitive deficits. Lastly, they show that core PC-proteins are required to integrate the rejuvenating effects of OCN in the hippocampus, thereby ameliorating age-related cognitive deficits. Altogether, these findings demonstrate a new paradigm for the neuron / systemic milieu communication and significantly advance our understanding of the regulatory mechanism mediating the rejuvenating effects of the pro-youth blood factor, OCN, in hippocampal neurons. This study also provides the foundation for novel therapeutic strategies to treat age-related cognitive decline, as well as neurological deficits in ciliopathies. Thus, the present invention relates to an activator of the primary cilia (PC) for use in the restoration and / or improvement of cognitive functions in a subject in need thereof.

The invention discloses a preparation method and application of a magnetic control cilia array, and the method comprises the steps: S1, taking a substrate coated with a hot melt adhesive layer, and placing magnetic control cilia on the substrate coated with the hot melt adhesive layer under the guidance of a magnetic field to obtain a to-be-fixed magnetic control cilia array; and S2, under a magnetic field condition, heating the hot melt adhesive layer of the to-be-fixed magnetic control cilia array, and curing to obtain the magnetic control cilia array. According to the preparation method and the application of the magnetic control cilia array, the preparation and planting processes of magnetic control cilia are separated into two independent steps, and a hot melt adhesive fixing method is adopted, so that a remarkable process breakthrough is realized, fundamental limitations of a traditional process in the aspects of efficiency, scale and applicability are solved, and the production cost of the magnetic control cilia array is reduced. And a feasible technical scheme is provided for industrial application of the intelligent surface.

The present application provides an inhibitor of primary cilium of an immune-related cell and an application thereof. The solution is to use an inhibitor of primary cilium of an immune-related cell containing an ERK1 / 2 inhibitor as an effective component.

The invention provides a Matrigel-free everted cilia organ and a culture method and application thereof.According to the method, a serum-free BPE-free amplification system, a Matrigel-free everted cilia organ and a high-throughput compatible culture method and an everted cilia differential medium are adopted, a culture system with definite chemical components is adopted for non-invasive sampling of a nasal swab, matrigel is not needed, and the Matrigel-free everted cilia organ is obtained. According to the method, the culture mode that the 96U-shaped bottom ultra-low adsorption pore plate / Agrrewell pore plate / cell ball honeycomb sheet pore plate is adopted for 3D differentiation culture to form the organoid is adopted, the purposes of completing amplification and cilia differentiation in a low-cost, standardized and high-throughput mode are achieved, and a feasible path is provided for subsequent large-scale drug screening and individualized precise diagnosis and treatment.

An oleanane-type triterpenoid or a glycoside thereof is useful as a compound having an effect of inducing formation of cilium and activating hair papilla cells or hair matrix cells.

The invention discloses nanoparticles and an electromagnetic transfection system for repairing cartilage injury as well as a preparation method and application of the nanoparticles and the electromagnetic transfection system, and belongs to the technical field of biological medicines. The preparation method of the nanoparticles comprises the following steps: S1, carrying out amination modification on SPIONs coated with silicon dioxide, so as to obtain aminated SPIONs; and S2, covalently binding small interfering RNA to the surface of the aminated SPIONs obtained in the S1 through a cross-linking agent, thereby obtaining the product. The si-SIRT3 (at) SPIONs nano delivery system is successfully constructed, non-invasive EMF exposure is combined, the problem that the cartilage tissue transfection efficiency is low is solved, SIRT3 overexpression, primary cilia injury and cartilage matrix degradation caused by factors such as T-2 toxin can be effectively reversed, the obvious cartilage protection and repair effects are shown in vitro and in vivo, and the cartilage tissue transfection system has the advantages of being high in biocompatibility, good in biocompatibility and good in biocompatibility. A novel targeted therapy normal form is provided for treating cartilage injury diseases such as Kaschin-Beck disease.

The invention provides a ciliate specific primer, a ciliate specific long primer suitable for an amplicon sequencing technology, a kit and application, and particularly belongs to the technical field of molecular biological detection and high-throughput gene sequencing. According to the present invention, the ciliate specific primer comprises a forward primer CS322F and a reverse primer CS819R, the ciliate specific long primer suitable for amplicon sequencing comprises a forward long primer LCS322F and a reverse long primer LCS819R. The ciliate specific primer disclosed by the invention has ciliate broad-spectrum universality, is adaptive to a short-read-length high-throughput sequencing method, and can be used for comprehensively analyzing the diversity and ecological functions of ciliate communities. Accurate detection and efficient analysis of ciliate communities can be realized, and technical support is provided for environmental monitoring, germplasm resource development and ecological assessment.

The invention discloses a preparation method of an airway organ, which comprises the following steps: dissociating and digesting airway tissues into single cells, and performing 2D multiplication culture to P3-P4 generations; uniformly mixing the cells subjected to 2D multiplication culture to P3-P4 generations with matrigel to obtain a cell-matrigel mixture; shearing the cell-matrigel mixture into monodisperse liquid drops by using a microfluidic technology, and curing the monodisperse liquid drops at 37 DEG C to obtain cell microspheres after curing; and inoculating the cell microspheres into an airway organoid culture medium, and culturing in a 5% CO2 incubator at 37 DEG C to obtain the airway organoid after the culture is finished. According to the method for constructing the airway organoid, the period is shortened, cell components such as airway epithelial cells and cilia cells are provided, and high-efficiency construction of the airway organoid can be achieved.

The present invention provides methods and kits for detecting cilium markers in samples and uses thereof for detecting and treating endothelial damage or dysfunction or vascular injury in the subject. Also provided are methods of sample processing, methods for detecting cilium markers on platelets, and methods of detecting traumatic brain injury.