335 results about "Acetylcysteine" patented technology

The invention discloses a liquefying agent, application thereof and a nucleic acid detection system, the liquefying agent comprises strong base, acetylcysteine and 2-(N-morpholine) ethanesulfonic acid; in the liquefying agent, the concentration of the strong base is 0.2 mol / L to 1 mol / L; the concentration of the acetylcysteine is 3 to 300 mmol / L; the concentration of the 2-(N-morpholine) ethanesulfonic acid ranges from 2 mmol / L to 5 mmol / L. Through the synergistic effect of the strong base, the acetylcysteine and the 2-(N-morpholine) ethanesulfonic acid, the liquefaction time can be remarkably shortened, the viscous biological sample treatment efficiency and speed can be improved, and the viscous biological sample treated by the liquefier can be directly used for nucleic acid amplification; and the viscous biological sample treated by the liquefying agent can be stored for a long time, the accuracy and reliability of nucleic acid detection are ensured by long-time stability, and the credibility of a detection result is improved.

The invention relates to the technical field of biomedicine, and discloses a human metapneumovirus culture method which comprises the following steps: a culture medium is prepared and put into a culture bottle, and the culture medium comprises a DMEM culture medium, glucose, sodium bicarbonate, an HEPES buffer solution, chondroitin sulfate and hyaluronic acid; host cells are selected; preparing a culture solution, wherein the culture solution comprises L-glutamine, bovine serum albumin, antibiotics and N-acetylcysteine; and inoculating the human metapneumovirus into the culture medium for adsorption and infection. The chondroitin sulfate and the hyaluronic acid are added into the culture medium, the chondroitin sulfate and the hyaluronic acid can be combined with glycosaminoglycans on the surfaces of the host cells to form a compound, interaction sites of viruses and the host cells are increased, then virus receptor expression on the surfaces of the host cells is enhanced, the cell membrane environment is optimized, and therefore the anti-tumor effect is achieved. Therefore, the attachment and entry efficiency of the hMPV is improved, and the rapid proliferation of the virus is obviously promoted.

The invention relates to the technical field of medical materials, in particular to an injection composition for endoscopic submucosal dissection and a preparation method and application thereof. The injection composition is prepared from the following raw materials: 1 to 5 w / v percent of sodium hydroxymethyl starch, 0.1 to 10 w / v percent of acetylcysteine, 0.2 to 4 w / v percent of polyvinylpyrrolidone, 0.001 to 0.1 w / v percent of coloring agent and the balance of phosphate buffer solution. The injection composition disclosed by the invention can be directly injected under mucous membranes, plays a role in upheaving diseased regions, has the advantages of long maintenance time and high upheaving degree, and has the effects of stopping bleeding and promoting wound healing.

The invention provides a tissue protection solution and application thereof. The tissue protection liquid comprises a basic culture medium; vitamins, wherein the vitamins comprise vitamin E; the antioxidant is prepared from at least one of N-acetylcysteine amide, superoxide dismutase and catalase; and a specific cell factor, wherein the specific cell factor comprises at least one of a ROCK inhibitor Y27632 and insulin. Therefore, when the tissue protection liquid is used for storing and transporting a tissue sample, the loss in the process from tissue sample separation to experiment starting can be reduced, the function and activity of tissue cells are ensured, and the success rate of establishment of various primary tissue cultures is effectively improved.

The invention relates to the technical field of nucleic acid extraction and purification, in particular to a DNA and RNA co-extraction reaction reagent and an extraction method, the reaction reagent comprises a nucleic acid protective agent RP, a sample lysis binding solution LB, a protein washing solution WB1, a nucleic acid rinsing solution WB2 and a nucleic acid eluent EB; the nucleic acid protective agent RP comprises DTT, mercaptoethanol, TCEP and sodium hydroxide; the sample cracking binding solution LB comprises Tris-HCl, benzenesulfonic acid, sodium citrate, guanidine hydrochloride, guanidine isothiocyanate, guanidine thiocyanate, urea, triton 100, SDS, potassium chloride, sodium chloride, bromhexine, N-acetylcysteine, ambroxol hydrochloride, isopropanol and absolute ethyl alcohol; the protein washing liquid WB1 comprises Tris-HCl, benzenesulfonic acid, sodium citrate, guanidine hydrochloride, guanidine isothiocyanate, guanidine thiocyanate, urea, triton 100, sodium chloride, isopropanol and absolute ethyl alcohol; the nucleic acid rinsing liquid WB2 is prepared from Tris-HCl, sodium chloride, isopropanol and absolute ethyl alcohol; and the nucleic acid eluent EB is nuclease-free water. The reaction reagent can be used for rapidly and sufficiently enriching and purifying DNA and RNA of a sample at the same time.

The invention provides a preparation method and application of mesenchymal stem cell exosomes, and the method is characterized in that N-acetylcysteine and quercetin are added into a culture medium used for culturing MSC cells, so that the MSC cells are stimulated to generate exosomes with anti-aging and anti-apoptosis effects. In addition, the exosome generated by stimulating the MSC through a culture mode of gradually reducing oxygen partial pressure further has an anti-aging effect; the adverse effects of acute oxidative stress, decline of multiplication capacity, unstable quality and the like in a low-oxygen state can be inhibited, and the activity of related components of cells and exosomes is not damaged. The method shows good anti-aging and anti-apoptosis effects in vitro, and a new thought is provided for treating aging diseases by the exosome in the future.

The present disclosure describes methods of administering N-acetylcysteine (NAC) via intranasal nose-to-brain administration. The effect of intranasal NAC nose-to-brain administration can be monitored using an analytical technique, for example, magnetic resonance spectroscopy (MRS). In some embodiments, intranasal nose-to-brain NAC can be used to treat a condition, for example, a brain injury.

Provided herein are compositions and methods for treating patients with Usher syndrome associated retinitis pigmentosa (UARP) comprising identifying that the subject has UARP and administering to the animal or human an effective amount of an N-acetylcysteine amide (NACA) sufficient to protect vision and inhibit degradation of Ellipsoid Zone (EZ) area and retinal sensitivity. In one example, the NACA formulation is doses at 50, 100, 200, 201, 210, 225, 250, 275, 300, 350, 400, 450, 500, 600, 700, 750, 800, 900, or 1,000 mg / day.

A pharmaceutical composition comprising sitagliptin or a pharmaceutically acceptable salt thereof, wherein the composition comprises less than 5 ppm of 7-Nitroso-3-(trifluoromethyl)- 5,6,7,8-tetrahydr

The invention discloses a method for effectively relieving soybean antigen protein Glycinin in fish feed and application of the method. The invention discloses application of N-acetylcysteine as a functional feed additive capable of relieving carp enteritis induced by soybean antigen protein Glycinin. The feed disclosed by the invention comprises a soybean antigen protein Glycinin and N-acetylcysteine (NAC). According to the present invention, 1.5-6.0 g / kg of NAC is added to a feed containing 80 g / kg of Glycinin, such that the fish growth, the feed utilization rate, the digestion function, the protein metabolism function, the immune function, the oxidation resistance and the intestinal morphology can be significantly improved, and the soybean antigen protein Glycinin-induced growth inhibition and the intestinal injury can be effectively relieved; the functional fish feed additive provided by the invention is relatively low in cost and easy to popularize, and is beneficial to development of novel green antibiotic-replacing additives, improvement of the utilization rate of soybean protein sources, reduction of feed cost and promotion of healthy culture of fishes.

The invention provides a method for simultaneously detecting at least two of cysteine, acetylcysteine and cystine or salts of amino acids in an amino acid composition. High performance liquid chromatography is adopted for detection, and chromatographic conditions comprise that octadecylsilane chemically bonded silica is adopted as a chromatographic column of a filler, and a mobile phase A and a mobile phase B are adopted for gradient elution; wherein the mobile phase A is a mixed solution of an ammonium sulfate solution and an ion pair reagent. According to the method disclosed by the invention, cysteine, acetylcysteine, cystine or salts of the amino acids in the amino acid composition can be separated from one another and can be effectively separated from other amino acid components or impurities, and the separation degree is high; cysteine, cystine and acetylcysteine in the reference substance and amino acid composition are stable in retention time and good in peak shape. The method provided by the invention has the advantages of strong specificity, good stability, high accuracy, accurate, reliable and effective detection result, good repeatability and high precision.

The mixture as the micronutrient composition is a combination of alpha-ketoglutaric acid, nicotinamide or nicotinic acid, L-proline, L-glutamine, L-citrulline, sodium pyruvate, pantothenic acid, L-cysteine or N-acetylcysteine, magnesium malate, magnesium citrate, and methionine. The mixture is effective for the treatment of diseases due to oxidative stress. More specifically, the mixtures and cores as micronutrient compositions are effective in reversing saccharification in diabetes mellitus.

The invention discloses a head and neck squamous cell carcinoma organ as well as a culture method, a culture medium and application thereof. The culture medium is prepared from the following components based on Advanced DMEM / F12: 1 percent of Antibiotic-Antibiotic, 1 percent of GlutaMAX, 1 percent of HEPES buffer solution, 50ng / ml of FGF-10, 50ng / ml of EGF, 1nM of bFGF, 500ng / ml of Wnt3a recombinant protein, 500ng / ml of Noggin recombinant protein, 0.1 mu g / ml of R-spondin-1 recombinant protein, 1mM of N-acetylcysteine, 10mM / ml of nicotinamide and 1x of B27 supplement. The culture medium and the culture method disclosed by the invention are particularly suitable for culturing head and neck squamous cell carcinoma organoid, and the obtained organoid has important significance for researching tumor generation, a unique invasive growth mechanism, prognosis, therapeutic targets, drug screening and the like, and has wide application prospects.

The invention provides a special culture medium for breast cancer organoid and a culture method thereof, and belongs to the technical field of primary cell culture. The special culture medium for the breast cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium is a DMEM (Dulbecco Modified Eagle Medium) / F12 culture medium. The culture additive is prepared from an epidermal growth factor, a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, transferrin, sodium selenite, A83-01, SB202190, an insulin-like growth factor 1, B27, nicotinamide and N-acetylcysteine. According to the culture medium, the culture cost is reduced, and meanwhile, the stability of tumor cell genomes in the culture process is improved.

The invention relates to the technical field of pharmacy, in particular to a saxagliptin oral preparation and a preparation method thereof. The preparation is prepared from the following components in parts by weight: 5 to 15 parts of saxagliptin or salt thereof, 15 to 30 parts of sodium alginate, 10 to 20 parts of chitosan, 10 to 25 parts of polylactic acid-glycolic acid copolymer (PLGA), 5 to 15 parts of polyvinyl alcohol, 3 to 8 parts of N-acetylcysteine, 2 to 6 parts of vitamin E and 1 to 5 parts of sodium lauryl sulfate. According to the present invention, the drug stability is significantly improved, the chitosan and the sodium alginate are subjected to the polyelectrolyte composite reaction to form the stable gel so as to effectively protect the active components of the drug, and the freeze drying treatment ensures the uniform dispersion and the efficient dissolution of the drug, improves the bioavailability, and enables the drug to be rapidly and fully absorbed and utilized; according to the preparation method, the flexibility of the preparation is improved, the preparation can be easily processed into different dosage forms such as tablets and capsules, the requirements of different patients are met, all the components act synergistically, and the medicine performance is jointly improved.

The invention discloses a method for evaluating combined toxicity of nano plastic and tamoxifen based on caenorhabditis elegans and a method for resisting drug toxicity caused by the nano plastic, a sample to be detected is an NGM solid culture medium of a mixture of the nano plastic and tamoxifen, and the caenorhabditis elegans is inoculated into the sample to be detected. The toxic effect of the joint exposure of the nano plastic and the tamoxifen on nematode movement, nerve, development and reproduction under the actual environment condition can be quickly and accurately evaluated, and a basis is provided for evaluation of human health risks. The invention provides a combined toxicity detection method which is suitable for laboratories, simple and convenient to operate and high in applicability by fully considering the problems that the toxicity is changed due to the chemical effect generated by nano-plastics and medicines and the in-vivo conditions are complicated and are difficult to observe, and provides a combined toxicity detection method which is suitable for laboratories and can be used for detecting the toxicity by supplementing N-acetylcysteine through diet. The effect of N-acetylcysteine in relieving tamoxifen toxicity caused by nano-plastics is further evaluated.

The invention discloses an electrotransfection solution for pig in-vitro fertilization embryos and application thereof, and belongs to the technical field of pig embryo in-vitro editing. The objective of the invention is to solve the technical problems of low in-vitro electrotransformation efficiency and large damage of pig embryos. The invention provides an electrotransfection liquid for a pig in-vitro fertilized embryo. The electrotransfection liquid is prepared from the following components: a basic liquid which is Opti-MEM, N-acetylcysteine, mannitol and a small-molecule inhibitor Y-27632. According to the technical scheme, the embryo damage in the electrotransfection process can be remarkably reduced while efficient delivery of the gene editing tool is ensured, and a key technical support is provided for establishing a stable and efficient pig embryo gene editing platform.

The invention belongs to the technical field of biochemical multi-item liquid quality control products containing glycocholic acid, and particularly relates to a biochemical multi-item liquid quality control product containing glycocholic acid and a preparation method of the biochemical multi-item liquid quality control product containing glycocholic acid. Comprising a buffer solution, a mixed anti-interference agent of acetylcysteine and cyclodextrin, a stabilizer, a surfactant and a preservative, and the concentration of the mixed anti-interference agent in the quality control product is 0.1%-2% (w / v); the analytes comprise glycocholic acid, total bile acid and other biochemical markers. In order to overcome the defects in the prior art, human serum is taken as a matrix, and a mixed anti-interference agent of acetylcysteine and cyclodextrin is added, so that the problems that glycocholic acid is easy to oxidize and degrade, cross-reacts with total bile acid and the stability of a liquid quality control product is insufficient are solved; the method can realize long-term stable and accurate detection of the liquid quality control product containing CG and multiple biochemical markers.

The present invention discloses an N-acetylcysteine xylitol injection and its preparation method, which comprises raw materials with the following mass-volume ratios: 0.020 - 0.035 g / mL of N-acetylcysteine, 0.015 - 0.030 g / mL of xylitol, 0.005 - 0.010 g / mL of pH regulator, and the balance being injection water. The method of the present invention can reduce the oxidation and degradation of N-acetylcysteine during the preparation of the medicinal solution by controlling the dissolution concentration, temperature and range of the residual headspace oxygen amount of N-acetylcysteine, and selecting a pH regulator with an appropriate concentration to control the pH value of the medicinal solution and controlling the preparation time, thereby improving the stability of the product quality and controlling the generation of related substances. The present invention significantly improves the quality stability of the N-acetylcysteine xylitol injection, ensures the safety of patients' medication, and makes the product have broad application prospects and value.

A powder contains a buffer salt and an amino acid, for example one or both of (i) least one glycine or functional derivative thereof or (ii) at least one N-acetylcysteine, L-cysteine or functional derivative thereof. Optionally the powder additionally includes at least one vitamin or mineral. Preferably the powder has at least one characteristic selected from the group consisting of (i) the amino acid is present in an amount that at least partially comprises the amino acid in a form selected from the group consisting of free form, dipeptides, tripeptides and mixtures thereof and (ii) the powder is substantially free of enzymes and antibodies. The powder can be reconstituted in a liquid containing an acidic component, such as juice or a polyphenol, and / or a protein, such as milk protein, plant protein, collagen or blends thereof. The buffer salt can minimize or prevent denaturation of protein and / or coagulation of protein upon reconstitution of the powder in the liquid.

The invention relates to a method for increasing the content of glutathione in egg yolk. According to the present invention, by using the effect that cysteine can combine glutamic acid and glycine in animal bodies so as to synthesize glutathione, the artificially synthesized N-acetylcysteine is added to the laying fowl feed, and the laying fowls are fed for 4 weeks, such that the glutathione content in the egg yolk is significantly increased, and can be up to 1.44 times of the glutathione content in the egg yolk of the common poultry egg, such that the glutathione content in the egg yolk can be significantly improved; and the nutritional value and the economic value of the poultry eggs are improved.

The invention belongs to the field of cell culture, and discloses an osteosarcoma organ culture medium and a gelatin planting method. The following components are added into the osteosarcoma organ-like culture medium: a B27 additive, an N-2 additive, a GlutaMAX additive, casein amino acid, N-acetylcysteine, nicotinamide, vitamin C, sodium pyruvate, human gastrin, Wnt3A, R-spondin 1 protein, Noggin protein, activin A protein, Forskolin, Y-27632, SB202190, CHIR99021, A83-01, h-EGF, FGF-10, HGF, VEGF A, IGF 1, IL-6, cyan-streptomycin and fetal calf serum. The osteosarcoma organoid culture medium provided by the invention can promote organoid cells to maintain relatively high multiplication capacity and maintain self-renewal, so that the amplification efficiency and the modeling success rate of the organoid are greatly improved.

Provided are a method, an agent, or the like for improving the stability of a medium and for improving the productivity of a recombinant protein. Specifically, provided are: a medium containing N-acetylcysteine and L-cysteine; a method for culturing a cell using a medium containing N-acetylcysteine and L-cysteine; a method for producing a recombinant protein using a medium containing N-acetylcysteine and L-cysteine; and a method, agent, or the like for stabilizing a medium containing L-cysteine using N-acetylcysteine.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention discloses a culture medium and a culture method of a breast cancer organoid. The culture medium comprises an Advanced DMEM / F12 basal culture medium and an additive, the additive is prepared from penicillin streptomycin / amphotericin B, a B-27 additive, L-glutamine amide, HEPES (Hydroxyethyl PES), N-Acetylcysteine, nicotinamide, SB202190, A83-01, FGF10 (Fibroblast Growth Factor 10), EGF (Epidermal Growth Factor), human Hergulin beta-1, Y27632, WNT-3A, R-spondin 1, NOGGIN (Nitrogen Growth Factor IN), FGF7 (Fibroblast Growth Factor 7) and beta-estradiol. The culture method of the breast cancer organoid comprises the step of using the breast cancer organoid culture medium, and the method specifically comprises the steps of tissue sample taking, primary tissue construction, breast cancer organoid liquid changing and breast cancer organoid subculture. According to the prepared breast cancer organoid culture medium, tumor cells taken from breast cancer tumors grow fast, the cell speed is high, and follow-up medicine screening experiments can be facilitated; after the breast cancer organoid culture medium is used for culturing the breast cancer organoid, the cellular morphology and the protein expression condition of the organoid are identified through HE and immunohistochemistry, and it can be seen that the organoid can well reduce the pathological characteristics of the tissue.

Described herein are compositions and methods for treating lung damage associated with cancer therapy, for example radiation-induced lung damage or chemotherapy-induced lung damage. Lung damage may be treated by administering heparin, optionally formulated with or administered concurrently with N-acetylcysteine. The compositions may be nebulized and administered via inhalation to treat lung damage.

The invention relates to a preparation method of an acetylcysteine oral solution. The preparation method comprises the following steps: preparing an initial solution from acetylcysteine, a thickening agent, a bacteriostatic agent, a stabilizer, a flavoring agent, a pH regulator and water; and filtering the primary solution to obtain a liquid medicine of the acetylcysteine oral solution, and then carrying out mechanical filling to obtain a finished product of the acetylcysteine oral solution. Before mechanical filling, primary nitrogen purging is carried out on the borosilicate bottle, so that the residual oxygen content in the borosilicate bottle is 12-21%; after the acetylcysteine oral solution is filled, the borosilicate bottle is subjected to nitrogen purging again, so that the residual oxygen content in the borosilicate bottle is 8 + / -0.5%; afterwards, the borosilicate bottle reaches the nitrogen dripping position through nitrogen curtain protection on the conveying track, liquid nitrogen dripping is carried out, finally, the residual oxygen content in the borosilicate bottle is 2%-3%, and capping is carried out. According to the invention, a packaging bottle is changed into a borosilicate bottle, and a liquid nitrogen dropping process is matched, so that the residual oxygen content in the sealed bottle can be reduced to 1.8%, and the generation of impurity C is inhibited.

The invention discloses a liquefying agent, application thereof and a nucleic acid detection system, the liquefying agent comprises strong base, acetylcysteine and 2-(N-morpholine) ethanesulfonic acid; in the liquefying agent, the concentration of the strong base is 0.2 mol / L to 1 mol / L; the concentration of the acetylcysteine is 3 to 300 mmol / L; the concentration of the 2-(N-morpholine) ethanesulfonic acid ranges from 2 mmol / L to 5 mmol / L. Through the synergistic effect of the strong base, the acetylcysteine and the 2-(N-morpholine) ethanesulfonic acid, the liquefaction time can be remarkably shortened, the viscous biological sample treatment efficiency and speed can be improved, and the viscous biological sample treated by the liquefier can be directly used for nucleic acid amplification; and the viscous biological sample treated by the liquefying agent can be stored for a long time, the accuracy and reliability of nucleic acid detection are ensured by long-time stability, and the credibility of a detection result is improved.

The invention relates to the technical field of biological medicines, in particular to application of N-acetylcysteine in preparation of a medicine for improving intestinal injuries caused by diabetic hyperthyroidism complicated diseases, and the N-acetylcysteine is used for preparing the medicine for improving the intestinal injuries caused by the diabetic hyperthyroidism complicated diseases. The traditional Chinese medicine composition can effectively relieve abnormal blood glucose rise of diabetic hyperthyroidism complications, and improve excessive ingestion and drinking conditions and intestinal injury.