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Creatine kinase isoenzyme detection reagent

A detection reagent, creatine kinase technology, applied in the field of biological reagents, can solve the problems affecting the accuracy of detection results, poor stability of reagents, affecting the application of reagents, etc., to achieve high clinical application value, high response activity, and ensure accuracy Effect

Active Publication Date: 2015-02-25
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conventional reagent detection, the accuracy of the test results is often affected by the presence of pyruvate, heparin and other interfering substances in the sample. At the same time, the anti-CK-M antibody in the reagent is unstable in the reagent, resulting in poor stability of the reagent, which in turn affects the quality of the test results. Application of reagents in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0069] Reagent R1 is composed of the following ingredients:

[0070] Imidazole buffer (pH=6.0) 50mmo1

[0071] Glucose 45mmo1

[0072] Nanoparticles 40mmol

[0073] N-acetylcysteine ​​30mmo1

[0074] Sodium edetate 8mmo1

[0075] Adenosine diphosphate 10mmo1

[0076] Coenzyme Ⅰ 2mmo1

[0077] Ribonucleotide 10mmo1

[0078] Pyruvate decarboxylase 0.5KU

[0079] Glucose 6-Phosphate Dehydrogenase 5KU

[0080] Hexokinase 2KU

[0081] Goat anti-human CK-M polyclonal antibody 50mL

[0082] Trehalose 10g

[0083] Add deionized water to 1L;

[0084] Reagent R2 is composed of the following ingredients:

[0085] Imidazole buffer (pH=6.0) 100mmo1

[0086] Phosphocreatine 120mmo1

[0087] Sodium dodecylbenzenesulfonate 20g

[0088] Sodium azide 10g

[0089] ...

Embodiment 2

[0096] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0097] Reagent R1 is composed of the following ingredients:

[0098] Imidazole buffer (pH=6.5) 80mmo1

[0099] Glucose 32mmo1

[0100] Nanoparticles 30mmol

[0101] N-acetylcysteine ​​25mmo1

[0102] Sodium edetate 5mmo1

[0103] Adenosine diphosphate 5mmo1

[0104] Coenzyme Ⅰ 4mmo1

[0105] Ribonucleotide 15mmo1

[0106] Pyruvate decarboxylase 1.2KU

[0107] Glucose 6-phosphate dehydrogenase 3KU

[0108] Hexokinase 4KU

[0109] Goat anti-human CK-M polyclonal antibody 30mL

[0110] Trehalose 5g

[0111] Add deionized water to 1L;

[0112] Reagent R2 is composed of the following ingredients:

[0113] Imidazole buffer (pH=7.0) 70mmo1

[0114] Phosphocreatine 170mmo1

[0115] Sodium dodecylbenzenesulfonate 10g

[0116] Proclin300 6g

[0117] Make up ...

Embodiment 3

[0121] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0122] Reagent R1 is composed of the following ingredients:

[0123] Imidazole buffer (pH=7.0) 100mmo1

[0124] Glucose 20mmo1

[0125] Nanoparticles 20mmol

[0126] N-acetylcysteine ​​20mmo1

[0127] Sodium edetate 1mmo1

[0128] Adenosine diphosphate 1mmo1

[0129] Coenzyme Ⅰ 6mmo1

[0130] Ribonucleotide 25mmo1

[0131] Pyruvate decarboxylase 2KU

[0132] Glucose 6-phosphate dehydrogenase 2KU

[0133] Hexokinase 6KU

[0134] Goat anti-human CK-M polyclonal antibody 10mL

[0135] Trehalose 1g

[0136] Add deionized water to 1L;

[0137] Reagent R2 is composed of the following ingredients:

[0138] Imidazole buffer (pH=6.5) 50mmo1

[0139] Phosphocreatine 220mmo1

[0140] Sodium dodecylbenzenesulfonate 5g

[0141] Proclin300 1g

[0142] Make up ...

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Abstract

The invention discloses a creatine kinase isoenzyme detection reagent. The reagent consists of a reagent R1 and a reagent R2 according to the volume ratio of 4 to 1, wherein the reagent R1 consists of an imidazole buffer liquid, glucose, nano particles, N-acetylcysteine, sodium ethylene diamine tetracetate, adenosine diphosphate, cozymase I, ribonucleotide, pyruvate decarboxylase, glucose 6-phosphate dehydrogenase, hexokinase, a goat anti-human CK-M polyclonal antibody and trehalose; the reagent R2 consists of an imidazole buffer liquid, phosphocreatine, sodium dodecyl benzene sulfonate and a preservative. The reagent disclosed by the invention is a creatine kinase isoenzyme detection reagent which is stable, accurate, and high in anti-interference, and has very high clinical application value.

Description

technical field [0001] The invention relates to a biological reagent, in particular to a detection reagent, in particular to a creatine kinase isoenzyme detection reagent. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) (ATP: Creatine N-phosphotransferase EC 2.7.3.2) usually exists in the cytoplasm and mitochondria of animal heart, muscle, and brain tissues, and is an enzyme related to intracellular energy operation and muscle contraction. , an important kinase directly related to the regeneration of adenosine triphosphate (ATP)1,2, which reversibly catalyzes the transphosphorylation reaction between creatine and ATP. Creatine kinase has four isozyme forms: muscle (MM), brain (BB), hybrid (MB) and mitochondrial (MiMi). The MM type mainly exists in various muscle cells, the BB type mainly exists in brain cells, the MB type mainly exists in cardiomyocytes, and the MiMi type mainly exists in myocardial and skeletal muscle mitochondria. The muscle creatine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/70
CPCG01N33/573
Inventor 谢清华王进谭柏清甘宜梧王绮李静包兴艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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