381 results about "Cysteine l" patented technology

The invention relates to a culture medium and a culture method of persistent DC-CIK cells and application of the culture medium and the culture method, and belongs to the technical field of cell culture. The invention provides a culture medium for persistent DC-CIK cells, which takes a serum-free culture medium as a solvent and comprises a recombinant human granulocyte macrophage colony stimulating factor, a recombinant human interleukin-12, a recombinant human tumor necrosis factor-alpha, a recombinant human interferon-gamma, L-cysteine, glutathione, ginseng polysaccharide and streptomycin sulfate. According to the culture medium and the culture method of the persistent DC-CIK cells, proliferation and growth of the DC-CIK cells can be promoted, and very high tumor killing activity of the DC-CIK cells can be maintained for a long time.

The invention discloses a culture medium for culturing a liver cancer micro-tumor model and a culture method thereof. The invention relates to the technical field of biology, and provides a culture medium for culturing a liver cancer micro-tumor model, and the culture medium is composed of an antibacterial and antifungal agent tri-antibody, HEPES, GlutaMax, a human recombinant protein EGF, FGF-10, HGF, IL-2, IL-15, R-spondin 1, calomel, gastrin, A83-01, N-acetyl-L-cysteine, nicotine, an N2 additive, B27 and a basal culture medium. The liver cancer micro-tumor model obtained by the method can accurately reflect various characteristics of the original focus of a patient, and is a good scientific research experiment model and a preclinical experiment model in the field of precise tumor diagnosis and treatment. It can be foreseen that the culture method has wide application prospects in the fields of liver cancer research and clinical diagnosis and treatment.

The invention provides a carbon nanotube modified polypropylene composite material and a preparation method thereof, and belongs to the technical field of antibacterial materials. According to the method, plant fibers are used as precursors, carbon nanotubes are prepared through high-temperature calcination, and the carbon nanotubes are subjected to surface activation treatment by using aids such as L-cysteine hydrochloride, sodium ascorbate and ethylenediamine, so that the dispersity and interface bonding force of the carbon nanotubes in a polypropylene matrix are remarkably improved. And then, mixing the modified carbon nano tube with polypropylene, polyhydric alcohol and a catalyst, reacting, and granulating through a double-screw extruder to prepare the composite material with excellent performance. The composite material disclosed by the invention has excellent mechanical properties, thermal stability, antibacterial property and conductivity, and is suitable for the fields of automobile parts, electronic device shells, building materials, medical apparatuses and instruments and the like. The method is simple in process, environment-friendly and efficient, renewable resources are fully utilized, and the method has a wide industrial application prospect.

The invention discloses a liquefying agent, application thereof and a nucleic acid detection system, the liquefying agent comprises strong base, acetylcysteine and 2-(N-morpholine) ethanesulfonic acid; in the liquefying agent, the concentration of the strong base is 0.2 mol / L to 1 mol / L; the concentration of the acetylcysteine is 3 to 300 mmol / L; the concentration of the 2-(N-morpholine) ethanesulfonic acid ranges from 2 mmol / L to 5 mmol / L. Through the synergistic effect of the strong base, the acetylcysteine and the 2-(N-morpholine) ethanesulfonic acid, the liquefaction time can be remarkably shortened, the viscous biological sample treatment efficiency and speed can be improved, and the viscous biological sample treated by the liquefier can be directly used for nucleic acid amplification; and the viscous biological sample treated by the liquefying agent can be stored for a long time, the accuracy and reliability of nucleic acid detection are ensured by long-time stability, and the credibility of a detection result is improved.

The invention relates to a bimolecular modified straw adsorbent as well as a preparation method and application thereof. The preparation method comprises the following steps: cleaning, drying and grinding straws, and sieving by a 100-mesh sieve; adding a polyethylene diamine solution, and carrying out stirring reaction at normal temperature; adding L-cysteine, adjusting the pH value to 4.5-5.5, and continuously stirring to react for 6-10 hours; and filtering, washing and drying to obtain the product. The preparation method has the advantages of simple steps, easily available raw materials and low requirements on equipment; the prepared bimolecular modified straw adsorbent has good removal effect and selectivity on Sb (III) and Sb (V), and is wide in pH value adaptation range. And a new technical means is provided for high-value conversion of straws and removal of antimony in water.

The invention relates to the technical field of biomedicine, and discloses a human metapneumovirus culture method which comprises the following steps: a culture medium is prepared and put into a culture bottle, and the culture medium comprises a DMEM culture medium, glucose, sodium bicarbonate, an HEPES buffer solution, chondroitin sulfate and hyaluronic acid; host cells are selected; preparing a culture solution, wherein the culture solution comprises L-glutamine, bovine serum albumin, antibiotics and N-acetylcysteine; and inoculating the human metapneumovirus into the culture medium for adsorption and infection. The chondroitin sulfate and the hyaluronic acid are added into the culture medium, the chondroitin sulfate and the hyaluronic acid can be combined with glycosaminoglycans on the surfaces of the host cells to form a compound, interaction sites of viruses and the host cells are increased, then virus receptor expression on the surfaces of the host cells is enhanced, the cell membrane environment is optimized, and therefore the anti-tumor effect is achieved. Therefore, the attachment and entry efficiency of the hMPV is improved, and the rapid proliferation of the virus is obviously promoted.

The invention discloses a washable easily-colored wool fabric treatment process, belongs to the technical field of wool fabric processing, and aims to solve the technical problem that the dyeing degree, the anti-felting performance and the color fastness of wool fabrics in the prior art need to be further improved. The method includes the following steps that disulfide bonds of wool fibers are opened through L-cysteine, the washable and easy-to-color wool fabric is obtained, the pretreated wool fibers prepared through the method are grafted with methoxy polyethylene glycol methacrylate under catalysis of hydrogen peroxide and horse radish peroxidase, modified wool fibers are obtained, and the washable and easy-to-color wool fabric is obtained. After the modified wool fibers are woven into the wool fabric, the wool fabric is respectively subjected to padding modification by using the modified dye solution and the finishing liquid, so that the degree of dyeing, the anti-felting performance and the color fastness of the wool fabric are improved.

The invention provides a tissue protection solution and application thereof. The tissue protection liquid comprises a basic culture medium; vitamins, wherein the vitamins comprise vitamin E; the antioxidant is prepared from at least one of N-acetylcysteine amide, superoxide dismutase and catalase; and a specific cell factor, wherein the specific cell factor comprises at least one of a ROCK inhibitor Y27632 and insulin. Therefore, when the tissue protection liquid is used for storing and transporting a tissue sample, the loss in the process from tissue sample separation to experiment starting can be reduced, the function and activity of tissue cells are ensured, and the success rate of establishment of various primary tissue cultures is effectively improved.

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The invention discloses a culture medium and a method for performing in-vitro 3D cell model construction on skin tissues. The invention provides a culture medium for constructing a skin tissue in-vitro 3D (three-dimensional) cell model. The culture medium is prepared from an antibacterial and antifungal agent tri-antibody, HEPES, GlutaMax, MEM non-essential amino acid, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, IL-2, IL-15, IGF-I, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotine, an N2 additive, B27, ITS-X, Y-27632, dexamethasone, calomel and a basal culture medium. The skin tissue in-vitro 3D cell model constructed by the invention has a wide application prospect in the fields of basic scientific research and clinical diagnosis and treatment of skin diseases.

A pharmaceutical composition comprising sitagliptin or a pharmaceutically acceptable salt thereof, wherein the composition comprises less than 5 ppm of 7-Nitroso-3-(trifluoromethyl)- 5,6,7,8-tetrahydr

The invention discloses application of double enzymes in one-step preparation of L-selenium methyl selenocysteine, and belongs to the technical field of biochemical engineering. In the method, racemic N-acetyl selenium methyl selenocysteine is used, and the L-selenium methyl selenocysteine with high optical purity is prepared in a green and efficient manner through co-catalysis of stereoselective L amino acid acylase and N-acetyl amino acid racemase. The catalyst is prepared through fermentation by adopting a one-bacterium two-enzyme method. Synchronous racemization and selective hydrolysis are realized by a one-step method, the theoretical conversion rate is 100%, the actual conversion rate is 95%, and the product is e.egt; the concentration of the substrate is 8%.

The present invention relates to a restructuring composition for damaged hair which comprises a plurality of ingredients and which can be used for the production of new finished formulations in the cosmetic field. The composition 5 comprises glycerine, hydrolysed glycosaminoglycans, low molecular weight hyaluronic acid and cysteine, the latter preferably in a salified form, preferably as hydrochloride.

The invention provides a stable acetylcysteine injection and a preparation method thereof. The acetylcysteine injection provided by the invention only contains a small amount of metal ion chelating agent, and can still improve the content of related substances in the preparation and obviously reduce the content of hydrogen sulfide in the preparation by using a novel package and performing terminal high-temperature sterilization under the condition of a pre-cleaning process. More importantly, in the long-term storage process of the acetylcysteine injection provided by the invention, the content of hydrogen sulfide presents a trend of continuously reducing.

The invention discloses a head and neck squamous cell carcinoma organ as well as a culture method, a culture medium and application thereof. The culture medium is prepared from the following components based on Advanced DMEM / F12: 1 percent of Antibiotic-Antibiotic, 1 percent of GlutaMAX, 1 percent of HEPES buffer solution, 50ng / ml of FGF-10, 50ng / ml of EGF, 1nM of bFGF, 500ng / ml of Wnt3a recombinant protein, 500ng / ml of Noggin recombinant protein, 0.1 mu g / ml of R-spondin-1 recombinant protein, 1mM of N-acetylcysteine, 10mM / ml of nicotinamide and 1x of B27 supplement. The culture medium and the culture method disclosed by the invention are particularly suitable for culturing head and neck squamous cell carcinoma organoid, and the obtained organoid has important significance for researching tumor generation, a unique invasive growth mechanism, prognosis, therapeutic targets, drug screening and the like, and has wide application prospects.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

The invention discloses a culture medium of a lacrimal gland organ and a culture method of the lacrimal gland organ, and the culture medium of the lacrimal gland organ is composed of a basic culture medium, R-spodin3, Noggin, B27, N-acetyl-L-cysteine, trehalose, vitamin C, glutathione, an adenylate cyclase activator, A8301, PGE2 and FGF10. The culture medium for lacrimal gland organ culture, provided by the invention, is prepared from various cell factor components according to an optimized proportion, and is simple and reasonable in components and rich in nutrition; when the culture medium is used for culturing the lacrimal gland organoid, the formation and growth of the organoid can be promoted, an in-vivo complex microenvironment is further simulated, the organoid is closer to an in-vivo physiological state, an organoid model is better constructed so as to evaluate the influence of drugs on lacrimal gland functions, and a new platform is provided for drug screening; and a new way is opened up for follow-up treatment of diseases such as xerophthalmia in the future.

The invention discloses an electrotransfection solution for pig in-vitro fertilization embryos and application thereof, and belongs to the technical field of pig embryo in-vitro editing. The objective of the invention is to solve the technical problems of low in-vitro electrotransformation efficiency and large damage of pig embryos. The invention provides an electrotransfection liquid for a pig in-vitro fertilized embryo. The electrotransfection liquid is prepared from the following components: a basic liquid which is Opti-MEM, N-acetylcysteine, mannitol and a small-molecule inhibitor Y-27632. According to the technical scheme, the embryo damage in the electrotransfection process can be remarkably reduced while efficient delivery of the gene editing tool is ensured, and a key technical support is provided for establishing a stable and efficient pig embryo gene editing platform.

The invention discloses low-resistance branch-sheet-shaped silver-coated copper powder, the copper powder is in a branch sheet shape, the surface of the branch-sheet-shaped copper powder is uniformly coated with a silver layer, the surface of the silver layer is coated with a chelate layer, and the chelate layer comprises cysteine and glutathione. The invention further discloses a preparation method of the low-resistance branch-sheet-shaped silver-coated copper powder. The preparation method comprises the following steps: plating silver on the surface of the branch-sheet-shaped copper powder to obtain an intermediate; and cleaning the intermediate with an aminothiol solution, drying, and then adding the dried intermediate into a mixed solution containing cysteine and glutathione for chelation reaction to obtain the low-resistance dendritic sheet-shaped silver-coated copper powder. The invention further discloses application of the low-resistance branch-sheet-shaped silver-coated copper powder in a coating. The invention further discloses a coating which comprises a film forming substance and the low-resistance branch-sheet-shaped silver-coated copper powder. The low-resistance branch-sheet-shaped silver-coated copper powder has good dispersity, oxidation resistance and conductivity in a coating, and the adhesive force of the coating can be improved.

The invention relates to the technical field of preparation of composite pigments, in particular to a preparation method of a high-temperature-resistant photolysis-resistant composite pigment and application of the high-temperature-resistant photolysis-resistant composite pigment in meat products. According to the technical scheme, the method comprises the following steps: preparing the following raw materials in parts by weight: pigment base materials: 5-18 parts of monascus red pigment, 4-12 parts of high-color-value capsanthin and 3-8 parts of beta-cyclodextrin embedded curcumin; the component A comprises the following components in parts by weight: 1-5 parts of ascorbyl palmitate, 2-8 parts of polyglycerol fatty acid ester, 0.5-3 parts of L-sodium ascorbate and 0.1-1.5 parts of nano titanium dioxide; and 1 to 4 parts of N-acetyl-L-cysteine. The ultra-high stability of the natural pigment under the conditions of high-temperature processing and strong light irradiation is realized, the processing tolerance and the final color development effect are balanced, the problem of uneven dyeing caused by early release of the traditional pigment is solved, the product quality and the application convenience are improved, and the unification of safety and efficacy is realized.

The invention relates to the technical field of amino acid compositions, and discloses a compound amino acid composition for enhancing telomerase activity, which comprises the following components (by weight): 20-25 parts of L-arginine; 15 to 20 parts of L-lysine; 25 to 30 parts of L-glutamine; 10 to 15 parts of L-serine; 5 to 8 parts of L-glycine; 3 to 5 parts of L-proline; 2 to 4 parts of N-acetyl-L-cysteine; 1 to 2 parts of L-tryptophan; 1 to 2 parts of beta-alanine; 2 to 3 parts of L-carnitine; 1-2 parts of taurine; through the synergistic effect of multiple mechanisms, the compound amino acid composition not only significantly enhances the telomerase activity (up to 195%), but also can maintain the telomere length, inhibit cell aging and provide antioxidant protection. The comprehensive regulation effect is derived from a unique metabolic network formed among the components, and mutual promotion and regulation are realized. Experimental results show that the long telomere length (8.3 kb vs control group 7.1 kb) can be maintained in 30-day long-term culture.

The invention discloses a tumor treatment molecule with a curative effect self-amplification function. Belongs to the technical field of biotechnology and new medicine, and comprises a peptide sequence Ac-Asp-Glu-Val-Asp-Cys (StBu)-Lys combined to a CBT part and a paclitaxel chemotherapy molecule connected to a lysine side chain. After the probe enters a tumor cell, the probe is specifically recognized by reducing glutathione highly expressed in the tumor cell, and an Ac-DEVDCK (PTX)-CBT structure is generated; then, a weak cell apoptosis phenomenon is initiated through a molecular grafted paclitaxel structure; the Ac-DEVD fragment is used as a high-specificity polypeptide substrate of cysteine-aspartic acid specific protease 3 which is necessary in a cell apoptosis pathway, and can be specifically cut by the cysteine-aspartic acid specific protease 3, so that the Ac-DEVDCK (PTX)-CBT is converted into CK (PTX)-CBT; then, CK (PTX)-CBT molecules are subjected to a CBT-Cys click reaction to form a cyclic dimer, and the cyclic dimer is self-assembled to form particles. According to the method, the affinity of the paclitaxel to the tubulin in the cytoskeleton is utilized, so that the tubulin in the cell is aggregated, the self-amplification effect of the curative effect of the cell is realized, and the cell is triggered to generate a pan-apoptosis effect.

The invention belongs to the field of industrial application of microalgae, and provides a method for improving astaxanthin produced by haematococcus pluvialis. Comprising the following steps: 1, culturing haematococcus pluvialis to a logarithmic phase; 2, the haematococcus pluvialis is diluted to a proper concentration, and a seed solution for producing astaxanthin from the haematococcus pluvialis is prepared; 3, respectively adding six pre-prepared inducers including creatine, glycine, 6-aminocaproic acid, glutamic acid, L-hydroxyproline and L-cysteine into the prepared haematococcus pluvialis liquid for induction; and 4, continuously filling sterile air into the haematococcus pluvialis liquid added with the inducer in a photobioreactor, culturing, and inducing haematococcus pluvialis cells to accumulate astaxanthin. The invention discloses a method for improving astaxanthin produced by haematococcus pluvialis. The haematococcus pluvialis can be promoted to accumulate a large amount of astaxanthin, and the production cycle of the astaxanthin is shortened. The method is easy to operate, high in efficiency, low in cost and completely suitable for industrial culture of haematococcus pluvialis.

The present invention discloses a culture medium for culturing a liver cancer microtumor model and a culture method thereof. The present invention relates to the field of biotechnology, and provides a culture medium for culturing a liver cancer microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant protein EGF, FGF‑10, HGF, IL‑2, IL‑15, R‑spondin 1, forskolin, gastrin, A83‑01, N‑acetyl‑L‑cysteine, nicotinamide, N2 additive, B27 and basal culture medium. The liver cancer microtumor model obtained by the method of the present invention can accurately reflect the various characteristics of the original lesion of the patient, and is a good scientific research experimental model and preclinical experimental model in the field of precise diagnosis and treatment of tumors. It can be foreseen that this culture method has broad application prospects in the research and clinical diagnosis and treatment of liver cancer.

The invention generally relates to a process for preparing a texturized plant-based food product. More specifically the invention relates to a process for preparing a texturized plant-based food product with a fibrous appearance and less plant protein off-taste having 0.1-5 wt % sugar and 0.05-1 wt % cysteine or glutathione or a combination thereof.

The micronutrient composition is a combination of at least 6 ingredients, for example alpha-Ketoglutaric Acid, Niacinamide and / or Niacin, L-Proline, L-Glutamine, L-Citrulline and Sodium Pyruvate, preferably at least 11 ingredients, for example alpha-Ketoglutaric Acid, Niacinamide and / or Niacin, L-Proline, L-Glutamine, L-Citrulline, Sodium Pyruvate, Pantothenic Acid, L-Cysteine and / or N-acetyl Cysteine, Magnesium Malate, Magnesium Citrate and Methionine, more preferably all of the ingredients which are selected from the group consisting of: alpha-Ketoglutaric Acid, Niacinamide and / or Niacin, L-Proline, L-Glutamine, L-Citrulline, Sodium Pyruvate, Pantothenic Acid, L-Cysteine and / or N-acetyl Cysteine, Magnesium Malate and / or Magnesium Citrate, Methionine and / or S- Adenosyl-Methionine (SAMe), Methylsulfonylmethane (MSM), and Wormwood powder and / or extract. The composition is effectively used to treat diseases due to oxidative stress. More specifically, the micronutrient composition is effective in reversing glycation in diabetes and thus in treating diabetes.

The invention discloses a culture medium for constructing a colorectal cancer peritoneal metastasis micro-tumor cell model and application of the culture medium. The invention relates to the technical field of biology, and provides a culture medium for culturing a micro-tumor model for colorectal cancer peritoneal metastasis. The invention discloses a cholera toxin immunopotentiator, which is composed of an antibacterial and antifungal agent three antibody, HEPES, GlutaMax, a human recombinant protein EGF, a human recombinant protein bFGF, a human recombinant protein HGF, a human recombinant protein Noggin, a human recombinant protein R-spondin 1, a human recombinant protein GDNF, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotinamide, an N2 additive, cholera toxin, B27, ITS-X, Y-27632, Galunissertib and a basal culture medium. The culture medium and the culture method disclosed by the invention can effectively improve the culture success rate of colorectal cancer peritoneal metastasis and focus combination.

A powder contains a buffer salt and an amino acid, for example one or both of (i) least one glycine or functional derivative thereof or (ii) at least one N-acetylcysteine, L-cysteine or functional derivative thereof. Optionally the powder additionally includes at least one vitamin or mineral. Preferably the powder has at least one characteristic selected from the group consisting of (i) the amino acid is present in an amount that at least partially comprises the amino acid in a form selected from the group consisting of free form, dipeptides, tripeptides and mixtures thereof and (ii) the powder is substantially free of enzymes and antibodies. The powder can be reconstituted in a liquid containing an acidic component, such as juice or a polyphenol, and / or a protein, such as milk protein, plant protein, collagen or blends thereof. The buffer salt can minimize or prevent denaturation of protein and / or coagulation of protein upon reconstitution of the powder in the liquid.

The invention provides a preparation method and application of a material for removing heavy metals in lithium battery waste liquid, and relates to the technical field of wastewater treatment. The method comprises the following steps: firstly, dispersing chitosan in acetic acid (1 Vol%), sequentially and respectively adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, N-hydroxysuccinimide and L-cysteine, and sealing and stirring in a dark place for 24 hours; the method comprises the following steps: dissolving chitosan (Cys-CTS) in deionized water, then precipitating a product with absolute ethyl alcohol, then centrifuging, washing with ethyl alcohol and deionized water, and carrying out vacuum drying on a sample at 50 DEG C to obtain L-cysteine modified chitosan (Cys-CTS); the method comprises the following steps: adding Cys-CTS and cetyltrimethylammonium bromide into heavy metal-containing wastewater, and magnetically stirring; then, polyacrylamide is added into the stirring liquid, and after full stirring is conducted for 30 min, standing and settling are conducted, and the heavy metal can be removed. The flocculating agent is directly used for flocculating and precipitating the heavy metal, the heavy metal removal efficiency is high, the cost is saved, the technological process is simple, and complex special equipment is not needed.

The invention relates to a method for increasing the content of glutathione in egg yolk. According to the present invention, by using the effect that cysteine can combine glutamic acid and glycine in animal bodies so as to synthesize glutathione, the artificially synthesized N-acetylcysteine is added to the laying fowl feed, and the laying fowls are fed for 4 weeks, such that the glutathione content in the egg yolk is significantly increased, and can be up to 1.44 times of the glutathione content in the egg yolk of the common poultry egg, such that the glutathione content in the egg yolk can be significantly improved; and the nutritional value and the economic value of the poultry eggs are improved.