87 results about "Acetyl cysteine" patented technology

Sublingual or buccal dosage forms comprise at least one antioxidant compound, such as N-acetyl cysteine (NAC), or a pharmaceutically acceptable salt thereof. The sublingual or buccal dosage forms are administered for treating central nervous system (CNS) disorders, such as traumatic brain injury or stroke.

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

Methods, formulations, and kits comprising an antioxidant compound for treatment of seizures, viral infections, encephalitis, exposure to chemical warfare agents, and / or exposure to a pesticide. An antioxidant such as N-acetyl cysteine (NAC) or a pharmaceutically acceptable salt is delivered by administration to a subject in need of treatment. Methods, formulations and kits may also comprise a cholinergic muscarinic antagonist, a cholinesterase reactivator, or both, and / or an inhibitor of a purinergic receptor, and / or a benzodiazepam. Kits for treating the subject comprising (a) at least one antioxidant compound or a pharmaceutically acceptable salt thereof, (b) an intranasal delivery device, and (c) an intramuscular delivery device. The pharmaceutical formulations can be pre-formulated into vials or cartridges that can be loaded into the intranasal delivery device or intramuscular delivery device.

The invention relates to the technical field of preparation of composite pigments, in particular to a preparation method of a high-temperature-resistant photolysis-resistant composite pigment and application of the high-temperature-resistant photolysis-resistant composite pigment in meat products. According to the technical scheme, the method comprises the following steps: preparing the following raw materials in parts by weight: pigment base materials: 5-18 parts of monascus red pigment, 4-12 parts of high-color-value capsanthin and 3-8 parts of beta-cyclodextrin embedded curcumin; the component A comprises the following components in parts by weight: 1-5 parts of ascorbyl palmitate, 2-8 parts of polyglycerol fatty acid ester, 0.5-3 parts of L-sodium ascorbate and 0.1-1.5 parts of nano titanium dioxide; and 1 to 4 parts of N-acetyl-L-cysteine. The ultra-high stability of the natural pigment under the conditions of high-temperature processing and strong light irradiation is realized, the processing tolerance and the final color development effect are balanced, the problem of uneven dyeing caused by early release of the traditional pigment is solved, the product quality and the application convenience are improved, and the unification of safety and efficacy is realized.

The invention relates to the technical field of amino acid compositions, and discloses a compound amino acid composition for enhancing telomerase activity, which comprises the following components (by weight): 20-25 parts of L-arginine; 15 to 20 parts of L-lysine; 25 to 30 parts of L-glutamine; 10 to 15 parts of L-serine; 5 to 8 parts of L-glycine; 3 to 5 parts of L-proline; 2 to 4 parts of N-acetyl-L-cysteine; 1 to 2 parts of L-tryptophan; 1 to 2 parts of beta-alanine; 2 to 3 parts of L-carnitine; 1-2 parts of taurine; through the synergistic effect of multiple mechanisms, the compound amino acid composition not only significantly enhances the telomerase activity (up to 195%), but also can maintain the telomere length, inhibit cell aging and provide antioxidant protection. The comprehensive regulation effect is derived from a unique metabolic network formed among the components, and mutual promotion and regulation are realized. Experimental results show that the long telomere length (8.3 kb vs control group 7.1 kb) can be maintained in 30-day long-term culture.

The invention belongs to the technical field of biological medicine, and particularly relates to a reagent for improving ovarian development and a preparation method and application thereof. The reagent for improving ovarian development is prepared by mixing the following raw materials in parts by weight: 20-30 parts of N-acetylcysteine; 5 to 10 parts of tranexamic acid; 3 to 8 parts of creatine; and 3000 parts of water. According to the reagent, the mRNA expression of PKD1P6 and follicle stimulating hormone receptors (FSHR) in ovarian tissues is up-regulated, the activity and functions of cumulus granular cells are enhanced, and apoptosis of the cumulus granular cells is inhibited, so that the effects of effectively promoting follicle development and inhibiting the apoptosis of the cumulus granular cells are achieved.

The present application relates to the technical field of biological medicine, and in particular to a cat triple vaccine immune adjuvant containing two kinds of cat interleukins and metabolic regulatory molecules, a composition and application. The composition comprises cat interleukin 15, cat interleukin 23 and a metabolic regulatory molecule, the metabolic regulatory molecule comprises beta-nicotinamide mononucleotide, beta-nicotinamide adenine dinucleotide disodium salt, N-acetyl cysteine and L-carnitine, and can be used for preparing a cat triple vaccine or an auxiliary preparation of the cat triple vaccine. The composition can effectively improve the protection of the vaccine, prolong the protection time of the vaccine and reduce adverse reactions; the vaccine can more durably and at a higher level reduce or control the reproductive infection of viruses, significantly improve the specific humoral immune level and memory response ability of animals, and better resist the invasion and spread of FPV, FCV and FHV-1.

The application relates to a compound medicine for delaying skeletal muscle aging and application thereof, and the medicinal active components in the compound medicine composition are composed of menadione bisulfite sodium and N-acetyl cysteine, and belong to the technical field of biological medicine. A novel drug combination mode is developed, an oxidant menadione bisulfite sodium (MBS) and an antioxidant N-acetyl-L-cysteine (NAC) are combined and given in different time periods to regulate the H2O2 circadian rhythm of the skeletal muscle of old mice, the changes of the muscle strength, histopathology and aging marker protein level of the skeletal muscle of the old mice are detected, and then the precise compound medicine capable of improving the skeletal muscle function of the old mice and delaying skeletal muscle aging is determined. The application provides an effective drug combination strategy for relieving or treating the delay of skeletal muscle aging, and has very significant meaning.

Sublingual or buccal dosage forms comprise at least one antioxidant compound, such as N-acetyl cysteine (NAC), or a pharmaceutically acceptable salt thereof. The sublingual or buccal dosage forms are administered for treating central nervous system (CNS) disorders, such as traumatic brain injury or stroke.

The invention provides an organoid culture medium, an organoid culture method and application of the organoid culture medium, the organoid culture medium comprises a first-stage culture medium, the first-stage culture medium comprises a first-stage basic culture medium and first-stage addition factors, and the first-stage addition factors comprise N-acetylcysteine, nicotinamide, a ROCK inhibitor, Noggin, R-spondin, EGF, HGF, Gastrin, FGF10, Blebbistatin, Forskolin, A83-01, LDN193189, DAPT, TGF-alpha and UDAC. By using the organoid culture medium, the liver organoid and the fatty liver organoid can be effectively obtained, and the obtained fatty liver organoid has obvious fat deposition, is sensitive to fatty liver treatment drugs, highly restores the physiological status when human body fatty liver diseases occur, and has high application value.

The application provides a kind of nasal mucosa in vitro culture method and culture fluid, the composition of the culture fluid includes Wnt / β-catenin signal pathway activator 0.1-20 μg / ml, fibroblast growth factor 10-150 ng / ml, TGF-β inhibitor 0.02-3 μg / ml, ROCK inhibitor 0.1-100 μmol / L, P38 MAPK inhibitor 0.1-1 μmol / L, serum-free additive 1X, N-acetyl cysteine 1-10 mmol / L, nicotinamide 1-20 mmol / L, GlutaMax additive 1X, antibiotic, DMEM / F12 culture medium 1X.The application can carry out in vitro culture to nasal mucosa, can keep at least 2 weeks of activity, and have complete epithelial barrier and immune system, can realize ex vivo nasal mucosa virus infection experiment.

This invention relates to a dual-mode chiral sensing platform, its preparation method, and its application for identifying amino acid enantiomers, belonging to the field of electrochemical sensing technology. The preparation method includes the following steps: preparing an MXene-gold nanoparticle-N-acetyl-L-cysteine ​​chiral solution; preparing an MXene-gold nanoparticle-N-acetyl-L-cysteine / L(D)-amino acid solution; preparing an MXene-gold nanoparticle-N-acetyl-L-cysteine ​​modified electrode; electrochemical chiral recognition of amino acid enantiomers; and temperature-based chiral recognition of amino acid enantiomers. This invention utilizes MXene, a two-dimensional nanomaterial with excellent conductivity and photothermal conversion capabilities, as a carrier to in-situ load gold nanoparticles. N-acetyl-L-cysteine ​​containing a thiol group at the end is captured through Au-S bonds. This chiral material can achieve chiral recognition of amino acid enantiomers through both electrochemical and temperature-based chiral sensing modes.

The application discloses a kind of preparation and application of broad-spectrum antibacterial antiviral nanometer zinc and compound nanometer zinc, belongs to the technical field of medical materials.The ginkgo leaf extract is mixed with zinc salt aqueous solution, reduction reaction is carried out under the action of ultrasonic and stirring, and nanometer zinc solution is obtained.Vitamin C and N-acetyl cysteine are added to nanometer zinc solution, reduction reaction is continuously carried out under the action of ultrasonic and stirring, then mixed with equal volume of water solution containing anti-inflammatory antipyretic analgesic component uniformly, and compound nanometer zinc solution is obtained.Nanometer zinc solution or compound nanometer zinc solution obtained is mixed with equal mass of sodium hyaluronate hydrogel uniformly to obtain nanometer zinc gel or compound nanometer zinc gel.The method of the application avoids the toxic side effects caused by using chemical reducing agents such as hydrazine hydrate and sodium borohydride for preparing nanometer zinc.The nanometer zinc and compound nanometer zinc of the application have excellent characteristics of broad-spectrum and high-efficiency antibacterial antiviral, and have good application prospect.

The invention discloses a special culture medium for enriching primary lung cancer stem cells and a cell culture and identification method, the cell culture medium is composed of a basic culture medium, a nutrient source, a pathway activation / inhibition component, an antioxidant component, an anti-apoptosis component, a proliferation promoting component and a cell differentiation related component, and the culture medium takes advanced DMEM / F12 as the basic culture medium. A serum-free cell culture additive in the cell culture medium improves the universality of the cell culture medium to lung cancer cells, Wnt / beta-catenin signals are amplified by a Wnt signal enhancer R-Spondin3 to maintain the characteristics of stem cells, proliferation and survival of the stem cells are jointly promoted by EGF, bFGF and vitamin B3 derivative Nicotinamide, cell apoptosis is inhibited by an ROCK inhibitor Y-27632 and EGF, and the cell culture medium is used for treating lung cancer cells. The TGF-beta receptor inhibitor A83-01 and the bFGF are used for inhibiting cell differentiation signals and maintaining the state of stem cells / progenitor cells, the antioxidant B27 and the N-Acetyl Cysteine are used for preventing oxidative stress damage and maintaining downward survival, and the proportion of all the components in the cell culture medium is adjusted.

The invention relates to a method for synthesizing an L-cysteine derivative by using a chemical enzyme method, and belongs to the field of amino acid manufacturing. The method comprises the following steps: by taking an L-cysteine solution prepared by enzymatic conversion as an initial raw material, directly acylating by using acetic anhydride under the condition of controlling the pH value to be 7.5-10, so as to prepare N-acetyl-L-cysteine; or under the condition of controlling the pH value to be 8-10, directly reacting with a chloroacetic acid solution to prepare carbocisteine. The raw material adopted by the invention is the L-cysteine solution prepared by enzymatic conversion, and the L-cysteine does not need to be separated and purified, so that the method has the advantages of low production cost and high efficiency, and also can avoid resource waste and environmental pollution caused by the separation and purification process in the traditional process. The operation method is simple, raw materials are easy to obtain, and wide application prospects are achieved.

The invention relates to the technical field of pet food, in particular to an eye-benefiting and liver-protecting fermentation type canine nutrient and a preparation method thereof, and the eye-benefiting and liver-protecting fermentation type canine nutrient comprises the following components: a liver-protecting component, an eye-benefiting component, a chitosan-calcium compound solution, beer yeast powder, honey, lactobacillus plantarum freeze-dried powder and bacillus subtilis freeze-dried powder. Porous starch of the liver protection component carries and protects N-acetylcysteine, and carnosine and quercetin modified by zein remove fermentation byproducts. The eye benefiting component is loaded and protected by octenyl succinic acid modified starch, and the dispersity and the stability of the xanthophyll are improved by grafting a hydrophobic cavity of sulfobutyl-beta-cyclodextrin. The laccase fixed on the surface of the porous starch catalytically oxidizes phenolic hydroxyl groups in a tannic acid-gelatin network on the surface of the eye benefiting component by using dissolved oxygen, a protective shell is formed by in-situ crosslinking and curing, and a chitosan-calcium compound solution closes the physical distance between the laccase and the tannic acid-gelatin network, so that the enzymatic oxidation reaction rate is increased.

The application belongs to the technical field of biological medicine, and discloses an oxidation-reduction response type nanoparticle as well as a preparation method and application thereof. The nanoparticle takes a cationic lipid compound and an amphiphilic block polymer containing a disulfide bond as a composite carrier, and co-encapsulates active ingredients siPD-L1 and N-acetyl cysteine. The nanoparticle can be responsively disintegrated in a high-reduction microenvironment of a tumor, synchronously release the two active ingredients, realize the dual effects of reversing immunosuppression and scavenging active oxygen respectively, and synergistically inhibit the growth and metastasis of residual tumors after radiofrequency ablation of liver cancer. The preparation process is simple, the nanoparticle has good stability, high targeting and high biological safety, and provides a new effective strategy for the treatment of residual tumors after radiofrequency ablation of liver cancer.

The invention discloses a manganese-doped carbon dot and a preparation method and application thereof.The preparation method comprises the steps that citric acid is dissolved in deionized water, then MnCl2. 4H2O, ethanediamine and N-acetylcysteine are sequentially added and fully dissolved, and a hydrothermal reaction is conducted to obtain the small-particle-shaped manganese-doped carbon dot. According to the invention, the inorganic substance MnCl2. 4H2O which is widely anti-inflammatory and anti-oxidation is utilized to participate in the preparation of the carbon dots through a simple hydrothermal synthesis method, and the obtained manganese-doped carbon dots have the advantages of excellent multi-enzyme activity, good biocompatibility and the like, and have good application prospects in the aspects of inflammatory enteritis, colitis recovery and the like.

The application discloses an anti-aging ergothioneine and nicotinamide compound preparation and a preparation method and application thereof, and belongs to the technical field of cosmetic health products. The compound preparation takes pyrroloquinoline quinone, ergothioneine and nicotinamide as the core, and is matched with betaine, trehalose, quercetin phospholipid complex, alpha-lipoic acid and N-acetyl cysteine in specific proportions. The anti-aging ergothioneine and nicotinamide compound preparation and the preparation method and application thereof are adopted, the components such as PQQ, ergothioneine, nicotinamide and quercetin phospholipid complex are synergistically used, and the synchronous activation of mitochondrial regeneration, energy metabolism, free radical targeted removal and mitochondrial mass control is realized for the first time. The compound preparation can be prepared into cosmetics for external use on the skin, and the effects of antioxidation and skin barrier repair can be achieved.

The application provides application of a reagent for detecting N-acetyl-L-cysteine in preparation of an ischemic brain injury diagnosis product, and relates to the technical field of in-vitro detection.The application provides a biomarker N-acetyl-L-cysteine (NAC) for ischemic brain injury diagnosis.ROC curve analysis shows that NAC has good distinguishing ability for ischemic brain injury, and can be used as a potential marker for ischemic brain injury.

The invention discloses a preparation method and application of a probiotic-loaded hydrogel precursor solution for treating stomach diseases, and belongs to the field of preparation of medicinal high polymer materials. Comprising the following steps: oxidizing hyaluronic acid with sodium periodate to obtain oxidized hyaluronic acid; reacting the oxidized hyaluronic acid with dopamine to prepare dopamine modified oxidized hyaluronic acid; the preparation method comprises the following steps: carrying out amidation reaction on chitosan and N-acetyl-L-cysteine to prepare thiolated chitosan; the preparation method comprises the following steps: mixing dopamine modified oxidized hyaluronic acid and a thiolated chitosan aqueous solution with probiotics, and carrying out a cross-linking reaction under oxidation and acidic conditions to obtain a hydrogel precursor solution; the hydrogel precursor solution can be taken orally and swallowed, can be further gelatinized in situ under the condition of stomach focus oxidation, has excellent adhesion, and can be effectively retained in the stomach and keep the activity of probiotics. In addition, the hydrogel can continuously remove excessive active oxygen in the stomach, relieve the inflammatory response of gastric mucosa and remarkably improve the microenvironment of the focus.

The invention discloses N-acetyl-L-cysteine for preventing and controlling apple fruit rust and application of the N-acetyl-L-cysteine, and relates to the technical field of agricultural production. According to the method, the N-acetyl-L-cysteine solution is prepared by mixing N-acetyl-L-cysteine, a surfactant and water, and the N-acetyl-L-cysteine solution is sprayed on the surfaces of young fruits in the key development period of the young fruits of the apples, namely 10-60 days after blooming, so that the prevention and treatment of fruit rust are realized, and the appearance quality and commodity value of the apples are improved. Meanwhile, as a natural compound, the N-acetyl-L-cysteine has excellent biocompatibility and safety, can be degraded by microorganisms, and conforms to the green chemistry concept.

The invention relates to a preparation method of entcartide hydrochloride. The preparation method comprises the following steps: step 1, synthesizing a D-acetylcysteine-L-Boc-cysteine methyl ester disulfide-D-alanine tripeptide compound through a liquid phase synthesis method; step 2, synthesizing a D-Pbf-arginine amide-D-alanine-D-Pbf-arginine-D-Pbf-arginine-D-Pbf-arginine pentapeptide compound by virtue of a liquid phase synthesis method, and synthesizing a D-Pbf-arginine pentapeptide compound by virtue of a liquid phase synthesis method; step 3, carrying out liquid phase condensation on the D-acetylcysteine-L-Boc-cysteine methyl ester disulfide-D-alanine tripeptide compound and the D-Pbf-arginine amide-D-alanine-D-Pbf-arginine-D-Pbf-arginine pentapeptide compound, so as to obtain an Itecartide precursor, and carrying out liquid phase condensation on the Itecartide precursor and the D-Pbf-arginine pentapeptide compound, so as to obtain the Itecartide. And step 4, carrying out deprotection, hydrolysis and salt conversion on the itecartide precursor to obtain the itecartide hydrochloride. The preparation method disclosed by the invention is simple to operate, low in cost, high in directional conversion rate, environment-friendly and high in product yield and purity.

The application belongs to the technical field of biotechnology, and particularly relates to a staged precise regulation in vitro expansion culture method of high-activity gamma delta T cells. In the synergistic activation stage, the complex activator containing zoledronic acid, IL-2, IL-15, IL-12 and IL-21 is used to significantly improve the early activation level of the gamma delta T cells; in the dynamic expansion stage, the concentration of metformin is adjusted step by step to support the continuous and efficient proliferation of the cells; in the gradient maturation stage, the combination system of N-acetyl cysteine, TWS119 and DL-alpha tocopheryl acetate is introduced to help maintain the functional activity and antioxidant state of the cells in the later culture stage. The gamma delta T cells with a purity higher than 90% and an expansion multiple more than 100 times can be obtained by using the method to culture for 10 days, and the cells exhibit significant killing activity to tumor cells, are suitable for the adoptive immunotherapy of tumor and infectious diseases, and have good clinical application prospects.

The invention discloses a composite fluorescent visual sensor as well as a preparation method and application thereof, and relates to the technical field of analysis and detection. The composite fluorescent visual sensor is prepared by the following method: firstly synthesizing cadmium telluride quantum dots modified by mercaptosuccinic acid and cadmium telluride quantum dots modified by N-acetyl-L-cysteine, and then mixing a porphyrin stock solution with the cadmium telluride quantum dots modified by N-acetyl-L-cysteine to obtain a chiral nano porphyrin solution. And finally, mixing with a pre-synthesized mercaptosuccinic acid modified cadmium telluride quantum dot to prepare the composite fluorescent visual sensor. The prepared quantum dot-nano porphyrin composite fluorescent visual sensor has good selectivity on L / D-theanine, has high sensitivity and anti-interference performance, can be used for carrying out visual accurate analysis and application on the content of L / D-theanine, and has the advantages of simplicity in operation, high sensitivity, low cost and the like.

The invention relates to a nanoparticle for use as a drug carrier and the synthesis method of this nanoparticle. In the invention, magnesium / aluminium (Mg / AI) mucoadhesive thiolated layered double hydroxide (nLDHT) nanoparticles are thiolated with L-cysteine and / or thioglycolic acid and / or 2-iminothiolane hydrochloride and / or glutathione and / or N-acetyl cysteine and / or 3-mercaptopropionic acid and / or 6- mercaptohexanoic acid. Thus, nLDHT interacts with the mucin on the surface of the targeted mucosal tissue for a longer period of time and acts with fewer side effects in the target tissues.

The invention discloses a guanidyl chitosan derivative capable of destroying a bacterial membrane as well as a preparation method and application of the guanidyl chitosan derivative, and belongs to the field of antibacterial materials. The derivative is prepared through four steps of reaction: reacting N, N-di-Boc-S-methylisothiourea with ethanolamine to prepare OBG; then, the OBG and lipoic acid are esterified under the catalysis of DCC / DMAP to obtain LBG; the preparation method comprises the following steps: reacting chitosan with N-acetylcysteine under the action of EDCI / HOBT to prepare CSSH; finally, LBG is subjected to trifluoroacetic acid deprotection to generate LGa, LGa and CSSH react in a hydrochloric acid-triethanolamine buffer solution and DMF mixed solution, and the guanidyl chitosan derivative is obtained. The derivative can adsorb negatively charged bacteria through electrostatic interaction, tear and destroy a bacterial membrane structure, effectively kill methicillin-resistant staphylococcus aureus and pseudomonas aeruginosa, is suitable for the field of biological sterilization, and provides support for development of novel antibacterial materials.

This invention relates to the field of microorganisms or enzymes, and discloses a T-cell activation and expansion medium and its culture process. The culture process includes: inoculating isolated T cells into a basal medium containing IL-2 and culturing them; on the 4th day of culture, adding IL-7, IL-15, and a defined molar ratio of nicotinamide and N-acetyl-L-cysteine ​​to the system; calculating the metabolic quotient by monitoring changes in lactate and glucose concentrations in the supernatant; and triggering a feeding action based on the metabolic quotient. During the feeding stage, the supplementation ratio of nicotinamide and N-acetyl-L-cysteine ​​is adjusted according to the threshold of the metabolic quotient. This invention achieves a precise coupling between mitochondrial energy drive and oxidative damage quenching, thereby solving the technical contradiction of difficulty in balancing expansion efficiency and phenotypic maintenance, effectively blocking the late-stage metabolic exhaustion pathway and directionally enriching memory subpopulations.

The invention provides a cyanide-free electrolyte for hard gold electroforming and a preparation process of the cyanide-free electrolyte, and belongs to the technical field of gold plating processes. The preparation process comprises the following steps: adding 4-dimethylaminopyridine for reaction to prepare an additive A; adding lithium tert-butoxide for reaction to prepare an additive B; preparing an AuCl3 solution; 5, 5-dimethyl hydantoin is dissolved, and all the components are added. The preparation method comprises the following steps: stirring and mixing N-acetyl-L-cysteine, triphenyl methanol and dichloromethane, mixing trifluoroacetic acid, performing reduced pressure distillation, purification and elution, then adding triethylamine, 4-dimethylaminopyridine and acetic anhydride for reaction, and adding trifluoroacetic acid and triethyl silane for reaction to prepare an additive A; after being added into a cyanide-free electrolyte, in the process of electroplating a gold layer, the gold plating solution can effectively promote the formation of crystal nucleuses, prevent the overgrowth of crystal grains and promote the refinement of gold crystal grains, and the fine and uniformly distributed crystal grain structure can remarkably improve the hardness and wear resistance of the plating layer.

The application discloses a culture medium for constructing a gastric cancer ovary metastasis microtumor model and application. The application relates to the technical field of biotechnology, and provides a culture medium for constructing a gastric cancer ovary metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, Follistatin, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotinamide, N2 additives, cholera toxin, B27, ITS-X, Y-27632, gastrin, prostaglandin E2, beta-estradiol, progesterone, Galunisertib and a basic culture medium. The culture success rate of the gastric cancer ovary metastasis microtumor model is effectively improved.