62 results about "Acetyl cysteine" patented technology

The invention belongs to the technical field of biological medicine, and particularly relates to a reagent for improving ovarian development and a preparation method and application thereof. The reagent for improving ovarian development is prepared by mixing the following raw materials in parts by weight: 20-30 parts of N-acetylcysteine; 5 to 10 parts of tranexamic acid; 3 to 8 parts of creatine; and 3000 parts of water. According to the reagent, the mRNA expression of PKD1P6 and follicle stimulating hormone receptors (FSHR) in ovarian tissues is up-regulated, the activity and functions of cumulus granular cells are enhanced, and apoptosis of the cumulus granular cells is inhibited, so that the effects of effectively promoting follicle development and inhibiting the apoptosis of the cumulus granular cells are achieved.

The present application relates to the technical field of biological medicine, and in particular to a cat triple vaccine immune adjuvant containing two kinds of cat interleukins and metabolic regulatory molecules, a composition and application. The composition comprises cat interleukin 15, cat interleukin 23 and a metabolic regulatory molecule, the metabolic regulatory molecule comprises beta-nicotinamide mononucleotide, beta-nicotinamide adenine dinucleotide disodium salt, N-acetyl cysteine and L-carnitine, and can be used for preparing a cat triple vaccine or an auxiliary preparation of the cat triple vaccine. The composition can effectively improve the protection of the vaccine, prolong the protection time of the vaccine and reduce adverse reactions; the vaccine can more durably and at a higher level reduce or control the reproductive infection of viruses, significantly improve the specific humoral immune level and memory response ability of animals, and better resist the invasion and spread of FPV, FCV and FHV-1.

The application relates to a compound medicine for delaying skeletal muscle aging and application thereof, and the medicinal active components in the compound medicine composition are composed of menadione bisulfite sodium and N-acetyl cysteine, and belong to the technical field of biological medicine. A novel drug combination mode is developed, an oxidant menadione bisulfite sodium (MBS) and an antioxidant N-acetyl-L-cysteine (NAC) are combined and given in different time periods to regulate the H2O2 circadian rhythm of the skeletal muscle of old mice, the changes of the muscle strength, histopathology and aging marker protein level of the skeletal muscle of the old mice are detected, and then the precise compound medicine capable of improving the skeletal muscle function of the old mice and delaying skeletal muscle aging is determined. The application provides an effective drug combination strategy for relieving or treating the delay of skeletal muscle aging, and has very significant meaning.

Sublingual or buccal dosage forms comprise at least one antioxidant compound, such as N-acetyl cysteine (NAC), or a pharmaceutically acceptable salt thereof. The sublingual or buccal dosage forms are administered for treating central nervous system (CNS) disorders, such as traumatic brain injury or stroke.

The invention provides an organoid culture medium, an organoid culture method and application of the organoid culture medium, the organoid culture medium comprises a first-stage culture medium, the first-stage culture medium comprises a first-stage basic culture medium and first-stage addition factors, and the first-stage addition factors comprise N-acetylcysteine, nicotinamide, a ROCK inhibitor, Noggin, R-spondin, EGF, HGF, Gastrin, FGF10, Blebbistatin, Forskolin, A83-01, LDN193189, DAPT, TGF-alpha and UDAC. By using the organoid culture medium, the liver organoid and the fatty liver organoid can be effectively obtained, and the obtained fatty liver organoid has obvious fat deposition, is sensitive to fatty liver treatment drugs, highly restores the physiological status when human body fatty liver diseases occur, and has high application value.

The application provides a kind of nasal mucosa in vitro culture method and culture fluid, the composition of the culture fluid includes Wnt / β-catenin signal pathway activator 0.1-20 μg / ml, fibroblast growth factor 10-150 ng / ml, TGF-β inhibitor 0.02-3 μg / ml, ROCK inhibitor 0.1-100 μmol / L, P38 MAPK inhibitor 0.1-1 μmol / L, serum-free additive 1X, N-acetyl cysteine 1-10 mmol / L, nicotinamide 1-20 mmol / L, GlutaMax additive 1X, antibiotic, DMEM / F12 culture medium 1X.The application can carry out in vitro culture to nasal mucosa, can keep at least 2 weeks of activity, and have complete epithelial barrier and immune system, can realize ex vivo nasal mucosa virus infection experiment.

This invention relates to a dual-mode chiral sensing platform, its preparation method, and its application for identifying amino acid enantiomers, belonging to the field of electrochemical sensing technology. The preparation method includes the following steps: preparing an MXene-gold nanoparticle-N-acetyl-L-cysteine ​​chiral solution; preparing an MXene-gold nanoparticle-N-acetyl-L-cysteine / L(D)-amino acid solution; preparing an MXene-gold nanoparticle-N-acetyl-L-cysteine ​​modified electrode; electrochemical chiral recognition of amino acid enantiomers; and temperature-based chiral recognition of amino acid enantiomers. This invention utilizes MXene, a two-dimensional nanomaterial with excellent conductivity and photothermal conversion capabilities, as a carrier to in-situ load gold nanoparticles. N-acetyl-L-cysteine ​​containing a thiol group at the end is captured through Au-S bonds. This chiral material can achieve chiral recognition of amino acid enantiomers through both electrochemical and temperature-based chiral sensing modes.

The application discloses a kind of preparation and application of broad-spectrum antibacterial antiviral nanometer zinc and compound nanometer zinc, belongs to the technical field of medical materials.The ginkgo leaf extract is mixed with zinc salt aqueous solution, reduction reaction is carried out under the action of ultrasonic and stirring, and nanometer zinc solution is obtained.Vitamin C and N-acetyl cysteine are added to nanometer zinc solution, reduction reaction is continuously carried out under the action of ultrasonic and stirring, then mixed with equal volume of water solution containing anti-inflammatory antipyretic analgesic component uniformly, and compound nanometer zinc solution is obtained.Nanometer zinc solution or compound nanometer zinc solution obtained is mixed with equal mass of sodium hyaluronate hydrogel uniformly to obtain nanometer zinc gel or compound nanometer zinc gel.The method of the application avoids the toxic side effects caused by using chemical reducing agents such as hydrazine hydrate and sodium borohydride for preparing nanometer zinc.The nanometer zinc and compound nanometer zinc of the application have excellent characteristics of broad-spectrum and high-efficiency antibacterial antiviral, and have good application prospect.

The invention discloses a special culture medium for enriching primary lung cancer stem cells and a cell culture and identification method, the cell culture medium is composed of a basic culture medium, a nutrient source, a pathway activation / inhibition component, an antioxidant component, an anti-apoptosis component, a proliferation promoting component and a cell differentiation related component, and the culture medium takes advanced DMEM / F12 as the basic culture medium. A serum-free cell culture additive in the cell culture medium improves the universality of the cell culture medium to lung cancer cells, Wnt / beta-catenin signals are amplified by a Wnt signal enhancer R-Spondin3 to maintain the characteristics of stem cells, proliferation and survival of the stem cells are jointly promoted by EGF, bFGF and vitamin B3 derivative Nicotinamide, cell apoptosis is inhibited by an ROCK inhibitor Y-27632 and EGF, and the cell culture medium is used for treating lung cancer cells. The TGF-beta receptor inhibitor A83-01 and the bFGF are used for inhibiting cell differentiation signals and maintaining the state of stem cells / progenitor cells, the antioxidant B27 and the N-Acetyl Cysteine are used for preventing oxidative stress damage and maintaining downward survival, and the proportion of all the components in the cell culture medium is adjusted.

The invention relates to the technical field of pet food, in particular to an eye-benefiting and liver-protecting fermentation type canine nutrient and a preparation method thereof, and the eye-benefiting and liver-protecting fermentation type canine nutrient comprises the following components: a liver-protecting component, an eye-benefiting component, a chitosan-calcium compound solution, beer yeast powder, honey, lactobacillus plantarum freeze-dried powder and bacillus subtilis freeze-dried powder. Porous starch of the liver protection component carries and protects N-acetylcysteine, and carnosine and quercetin modified by zein remove fermentation byproducts. The eye benefiting component is loaded and protected by octenyl succinic acid modified starch, and the dispersity and the stability of the xanthophyll are improved by grafting a hydrophobic cavity of sulfobutyl-beta-cyclodextrin. The laccase fixed on the surface of the porous starch catalytically oxidizes phenolic hydroxyl groups in a tannic acid-gelatin network on the surface of the eye benefiting component by using dissolved oxygen, a protective shell is formed by in-situ crosslinking and curing, and a chitosan-calcium compound solution closes the physical distance between the laccase and the tannic acid-gelatin network, so that the enzymatic oxidation reaction rate is increased.

The application belongs to the technical field of biological medicine, and discloses an oxidation-reduction response type nanoparticle as well as a preparation method and application thereof. The nanoparticle takes a cationic lipid compound and an amphiphilic block polymer containing a disulfide bond as a composite carrier, and co-encapsulates active ingredients siPD-L1 and N-acetyl cysteine. The nanoparticle can be responsively disintegrated in a high-reduction microenvironment of a tumor, synchronously release the two active ingredients, realize the dual effects of reversing immunosuppression and scavenging active oxygen respectively, and synergistically inhibit the growth and metastasis of residual tumors after radiofrequency ablation of liver cancer. The preparation process is simple, the nanoparticle has good stability, high targeting and high biological safety, and provides a new effective strategy for the treatment of residual tumors after radiofrequency ablation of liver cancer.

The invention discloses a manganese-doped carbon dot and a preparation method and application thereof.The preparation method comprises the steps that citric acid is dissolved in deionized water, then MnCl2. 4H2O, ethanediamine and N-acetylcysteine are sequentially added and fully dissolved, and a hydrothermal reaction is conducted to obtain the small-particle-shaped manganese-doped carbon dot. According to the invention, the inorganic substance MnCl2. 4H2O which is widely anti-inflammatory and anti-oxidation is utilized to participate in the preparation of the carbon dots through a simple hydrothermal synthesis method, and the obtained manganese-doped carbon dots have the advantages of excellent multi-enzyme activity, good biocompatibility and the like, and have good application prospects in the aspects of inflammatory enteritis, colitis recovery and the like.

The application discloses an anti-aging ergothioneine and nicotinamide compound preparation and a preparation method and application thereof, and belongs to the technical field of cosmetic health products. The compound preparation takes pyrroloquinoline quinone, ergothioneine and nicotinamide as the core, and is matched with betaine, trehalose, quercetin phospholipid complex, alpha-lipoic acid and N-acetyl cysteine in specific proportions. The anti-aging ergothioneine and nicotinamide compound preparation and the preparation method and application thereof are adopted, the components such as PQQ, ergothioneine, nicotinamide and quercetin phospholipid complex are synergistically used, and the synchronous activation of mitochondrial regeneration, energy metabolism, free radical targeted removal and mitochondrial mass control is realized for the first time. The compound preparation can be prepared into cosmetics for external use on the skin, and the effects of antioxidation and skin barrier repair can be achieved.

The application provides application of a reagent for detecting N-acetyl-L-cysteine in preparation of an ischemic brain injury diagnosis product, and relates to the technical field of in-vitro detection.The application provides a biomarker N-acetyl-L-cysteine (NAC) for ischemic brain injury diagnosis.ROC curve analysis shows that NAC has good distinguishing ability for ischemic brain injury, and can be used as a potential marker for ischemic brain injury.

The invention discloses a preparation method and application of a probiotic-loaded hydrogel precursor solution for treating stomach diseases, and belongs to the field of preparation of medicinal high polymer materials. Comprising the following steps: oxidizing hyaluronic acid with sodium periodate to obtain oxidized hyaluronic acid; reacting the oxidized hyaluronic acid with dopamine to prepare dopamine modified oxidized hyaluronic acid; the preparation method comprises the following steps: carrying out amidation reaction on chitosan and N-acetyl-L-cysteine to prepare thiolated chitosan; the preparation method comprises the following steps: mixing dopamine modified oxidized hyaluronic acid and a thiolated chitosan aqueous solution with probiotics, and carrying out a cross-linking reaction under oxidation and acidic conditions to obtain a hydrogel precursor solution; the hydrogel precursor solution can be taken orally and swallowed, can be further gelatinized in situ under the condition of stomach focus oxidation, has excellent adhesion, and can be effectively retained in the stomach and keep the activity of probiotics. In addition, the hydrogel can continuously remove excessive active oxygen in the stomach, relieve the inflammatory response of gastric mucosa and remarkably improve the microenvironment of the focus.

The invention relates to a preparation method of entcartide hydrochloride. The preparation method comprises the following steps: step 1, synthesizing a D-acetylcysteine-L-Boc-cysteine methyl ester disulfide-D-alanine tripeptide compound through a liquid phase synthesis method; step 2, synthesizing a D-Pbf-arginine amide-D-alanine-D-Pbf-arginine-D-Pbf-arginine-D-Pbf-arginine pentapeptide compound by virtue of a liquid phase synthesis method, and synthesizing a D-Pbf-arginine pentapeptide compound by virtue of a liquid phase synthesis method; step 3, carrying out liquid phase condensation on the D-acetylcysteine-L-Boc-cysteine methyl ester disulfide-D-alanine tripeptide compound and the D-Pbf-arginine amide-D-alanine-D-Pbf-arginine-D-Pbf-arginine pentapeptide compound, so as to obtain an Itecartide precursor, and carrying out liquid phase condensation on the Itecartide precursor and the D-Pbf-arginine pentapeptide compound, so as to obtain the Itecartide. And step 4, carrying out deprotection, hydrolysis and salt conversion on the itecartide precursor to obtain the itecartide hydrochloride. The preparation method disclosed by the invention is simple to operate, low in cost, high in directional conversion rate, environment-friendly and high in product yield and purity.

The application belongs to the technical field of biotechnology, and particularly relates to a staged precise regulation in vitro expansion culture method of high-activity gamma delta T cells. In the synergistic activation stage, the complex activator containing zoledronic acid, IL-2, IL-15, IL-12 and IL-21 is used to significantly improve the early activation level of the gamma delta T cells; in the dynamic expansion stage, the concentration of metformin is adjusted step by step to support the continuous and efficient proliferation of the cells; in the gradient maturation stage, the combination system of N-acetyl cysteine, TWS119 and DL-alpha tocopheryl acetate is introduced to help maintain the functional activity and antioxidant state of the cells in the later culture stage. The gamma delta T cells with a purity higher than 90% and an expansion multiple more than 100 times can be obtained by using the method to culture for 10 days, and the cells exhibit significant killing activity to tumor cells, are suitable for the adoptive immunotherapy of tumor and infectious diseases, and have good clinical application prospects.

The invention discloses a composite fluorescent visual sensor as well as a preparation method and application thereof, and relates to the technical field of analysis and detection. The composite fluorescent visual sensor is prepared by the following method: firstly synthesizing cadmium telluride quantum dots modified by mercaptosuccinic acid and cadmium telluride quantum dots modified by N-acetyl-L-cysteine, and then mixing a porphyrin stock solution with the cadmium telluride quantum dots modified by N-acetyl-L-cysteine to obtain a chiral nano porphyrin solution. And finally, mixing with a pre-synthesized mercaptosuccinic acid modified cadmium telluride quantum dot to prepare the composite fluorescent visual sensor. The prepared quantum dot-nano porphyrin composite fluorescent visual sensor has good selectivity on L / D-theanine, has high sensitivity and anti-interference performance, can be used for carrying out visual accurate analysis and application on the content of L / D-theanine, and has the advantages of simplicity in operation, high sensitivity, low cost and the like.

The invention relates to a nanoparticle for use as a drug carrier and the synthesis method of this nanoparticle. In the invention, magnesium / aluminium (Mg / AI) mucoadhesive thiolated layered double hydroxide (nLDHT) nanoparticles are thiolated with L-cysteine and / or thioglycolic acid and / or 2-iminothiolane hydrochloride and / or glutathione and / or N-acetyl cysteine and / or 3-mercaptopropionic acid and / or 6- mercaptohexanoic acid. Thus, nLDHT interacts with the mucin on the surface of the targeted mucosal tissue for a longer period of time and acts with fewer side effects in the target tissues.

The invention discloses a guanidyl chitosan derivative capable of destroying a bacterial membrane as well as a preparation method and application of the guanidyl chitosan derivative, and belongs to the field of antibacterial materials. The derivative is prepared through four steps of reaction: reacting N, N-di-Boc-S-methylisothiourea with ethanolamine to prepare OBG; then, the OBG and lipoic acid are esterified under the catalysis of DCC / DMAP to obtain LBG; the preparation method comprises the following steps: reacting chitosan with N-acetylcysteine under the action of EDCI / HOBT to prepare CSSH; finally, LBG is subjected to trifluoroacetic acid deprotection to generate LGa, LGa and CSSH react in a hydrochloric acid-triethanolamine buffer solution and DMF mixed solution, and the guanidyl chitosan derivative is obtained. The derivative can adsorb negatively charged bacteria through electrostatic interaction, tear and destroy a bacterial membrane structure, effectively kill methicillin-resistant staphylococcus aureus and pseudomonas aeruginosa, is suitable for the field of biological sterilization, and provides support for development of novel antibacterial materials.

This invention relates to the field of microorganisms or enzymes, and discloses a T-cell activation and expansion medium and its culture process. The culture process includes: inoculating isolated T cells into a basal medium containing IL-2 and culturing them; on the 4th day of culture, adding IL-7, IL-15, and a defined molar ratio of nicotinamide and N-acetyl-L-cysteine ​​to the system; calculating the metabolic quotient by monitoring changes in lactate and glucose concentrations in the supernatant; and triggering a feeding action based on the metabolic quotient. During the feeding stage, the supplementation ratio of nicotinamide and N-acetyl-L-cysteine ​​is adjusted according to the threshold of the metabolic quotient. This invention achieves a precise coupling between mitochondrial energy drive and oxidative damage quenching, thereby solving the technical contradiction of difficulty in balancing expansion efficiency and phenotypic maintenance, effectively blocking the late-stage metabolic exhaustion pathway and directionally enriching memory subpopulations.

The application discloses a culture medium for constructing a gastric cancer ovary metastasis microtumor model and application. The application relates to the technical field of biotechnology, and provides a culture medium for constructing a gastric cancer ovary metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, Follistatin, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotinamide, N2 additives, cholera toxin, B27, ITS-X, Y-27632, gastrin, prostaglandin E2, beta-estradiol, progesterone, Galunisertib and a basic culture medium. The culture success rate of the gastric cancer ovary metastasis microtumor model is effectively improved.

The application discloses a creatine kinase determination kit and a use method thereof. The creatine kinase determination kit comprises an R1 component and an R2 component. The R1 component comprises oxidized glutathione and auxiliary material 1. The R2 component comprises reduced coenzyme II, glucose-6-phosphate dehydrogenase, creatine phosphate, glutathione reductase and auxiliary material 2. The oxidized glutathione (GSSG) is reduced into reduced glutathione (GSH) by catalytic degradation of glutathione reductase (GR), and the reduced glutathione contains -SH, thereby activating CK. By the above method, the -SH compound N-acetyl-L-cysteine does not need to be added, thereby fundamentally solving the problem that -SH is gradually oxidized and deactivated, greatly improving the long-term stability of the reagent, and prolonging the effective period of the reagent.

The invention relates to the technical field of health care, in particular to a gamma-aminobutyric acid emotion regulation composition and a preparation process thereof, and the gamma-aminobutyric acid emotion regulation composition comprises the following components in parts by weight: gamma-aminobutyric acid particles, tea leaf theanine, xylaria nigripes polysaccharide, N-acetylcysteine modified chitosan, vitamin B6, a trehalose stabilizer and soybean phosphatidylserine. According to the invention, the synergistic effect of stability improvement, delivery efficiency optimization and efficacy synergistic enhancement is formed through cooperation of the components instead of simple superposition of the components. Due to the slow release characteristic of the gamma-aminobutyric acid particles, the concentration of gamma-aminobutyric acid in a central nervous system is maintained within an effective treatment window, and a time window is provided for the synergistic effect of xylaria nigripes polysaccharide and soybean phosphatidylserine; the tea leaf theanine inhibits the release of excitatory transmitters and reduces the action load of gamma-aminobutyric acid, so that a small amount of gamma-aminobutyric acid can achieve an ideal regulation effect; the xylaria nigripes polysaccharide improves the sensitivity of nerve cells to gamma-aminobutyric acid, which is equivalent to the effect of amplifying gamma-aminobutyric acid.

The invention discloses a health-care product composition for blood purification and liver and gall detoxification as well as a preparation method and application thereof, and belongs to the technical field of health-care products. The health-care product composition for blood purification and liver and gall detoxification provided by the invention is prepared from the following components in parts by mass: 10 to 40 parts of fermented barley leaf powder, 5 to 20 parts of a silibinin phospholipid complex, 5 to 15 parts of a dandelion extract, 5 to 15 parts of an artichoke extract, 5 to 10 parts of curcumin, 2 to 8 parts of alpha-lipoic acid and 1 to 5 parts of N-acetylcysteine. According to the composition, a four-dimensional synergistic purification system of intestinal adsorption purification, liver biochemical detoxification, bile excretion promotion and whole-course antioxidant protection is constructed, and the components are mutually promoted and enhanced at different time and space and different target spots to synergistically achieve the double effects of blood purification and liver and gallbladder detoxification.

The application discloses a culture medium for constructing a liver cancer lung metastasis microtumor model and application thereof. The application relates to the technical field of biology, and provides a culture medium for constructing a liver cancer lung metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant proteins EGF, HGF, IL-2, IL-15, R-spondin1, CXCL12, recombinant human lung surfactant proteins SP-A, SP-D, forskolin, A83-01, N-acetyl-L-cysteine, nicotinamide, N2 additives, B27 and a basic culture medium. The culture success rate of the liver cancer lung metastasis microtumor model is effectively improved.

The application discloses a breeding method for promoting the crispness of tilapia fish meat and application thereof, and relates to the technical field of aquaculture. In the juvenile stage of the tilapia, a complex package 1 is added in the basic feed; in the adult stage, a complex package 2 is added in the crisp feed formula, the complex package 1 comprises N-acetyl cysteine, alpha-lipoic acid, lysed lecithin and bile acid, and the complex package 2 comprises alpha-ketoglutaric acid and sodium acetate. The application provides a phased feeding scheme according to the physiological characteristics of the tilapia in different growth periods, and breaks through to provide a new direction for improving the quality of the crisp tilapia meat, further increases the muscle texture by improving the collagen cross-linking degree of the fish meat, achieves the effect of fast and crisp, and the application integrates two kinds of complex packages, each of which is compounded with multiple additives, can achieve a synergistic effect, and avoids the risk of side effects caused by excessive single additive.

A topical composition comprising a nanonised L-glutathione and cyclodextrin complex, together with ascorbic acid in a weight ratio of glutathione to ascorbic acid from 7:1 to 15:1 and from 1:1 to 1:15 and a further compound comprising dexpanthenol, thiamine, benzalkonium chloride, sodium hyaluronate or acetyl-L-cysteine, which composition is topically administered for treating the symptoms of viral infection including COVID-19 and influenza, and for treating herpes simplex, herpes zoster, post herpetic neuralgia, genital herpes, alcohol detoxification, high triglycerides, age or liver spots and lupus.

The invention relates to the technical field of organoid culture, and discloses an induction culture medium for culturing a pig lung organoid. Advanced DMEM / F12 is used as a basic culture solution of the culture medium; the invention relates to a basic fibroblast growth factor composition, which is prepared from the following added components with the final concentration: 1500ng / mL of R-Spondinin, 25ng / mL of basic fibroblast growth factor 7 (FGF7), 10100ng / mL of basic fibroblast growth factor, 100ng / mL of Noggin protein, 50ng / mL of epidermal growth factor, 50ng / mL of Wnt3a protein, 500nM of TGF-beta pathway inhibitor, 5mM of ROCK inhibitor, 500nM of p38MAPK inhibitor, 1X of B27 supplement, 1.25 mM of N-acetyl-L-cysteine, 10mM of nicotinamide, 1X of glutamine supplement GlutaMAX1X, 1X of HEPES buffer solution and 1X of broad-spectrum antibiotic (Primocin). According to the method disclosed by the invention, the lung organ with the complex multi-cell lineage is efficiently and stably constructed from the pig lung adult tissue by a one-step method. The obtained organoid is high in biomimetic property and good in stability, provides a tool platform for pig lung development research, disease model construction, drug toxicology testing and regenerative medicine research, and has great scientific research value and wide application prospects.

The invention discloses a liver organoid culture medium and a culture method thereof, a liver organoid and application, and belongs to the technical field of biological medicine. The liver organoid culture medium is a basal culture medium containing stigmasterol, fetal calf serum, a B27 supplement, N-acetylcysteine, nicotinamide, recombinant human leucine 15-gastrin I, recombinant mouse epidermal growth factors, recombinant human fibroblast growth factors, A83-01, DAPT (Diamantadine Phosphate), dexamethasone and double antibodies PS (Poly Styrene). The stigmasterol is added, so that the growth and amplification of liver organs can be promoted. By applying the liver organoid culture medium, a simple, universal and efficient liver organoid culture method is provided, and the core bottleneck that amplification is difficult in liver organoid culture is solved.