12 results about "Acetyl cysteine" patented technology

Sublingual or buccal dosage forms comprise at least one antioxidant compound, such as N-acetyl cysteine (NAC), or a pharmaceutically acceptable salt thereof. The sublingual or buccal dosage forms are administered for treating central nervous system (CNS) disorders, such as traumatic brain injury or stroke.

The application provides application of a reagent for detecting N-acetyl-L-cysteine in preparation of an ischemic brain injury diagnosis product, and relates to the technical field of in-vitro detection.The application provides a biomarker N-acetyl-L-cysteine (NAC) for ischemic brain injury diagnosis.ROC curve analysis shows that NAC has good distinguishing ability for ischemic brain injury, and can be used as a potential marker for ischemic brain injury.

The application belongs to the technical field of biotechnology, and particularly relates to a staged precise regulation in vitro expansion culture method of high-activity gamma delta T cells. In the synergistic activation stage, the complex activator containing zoledronic acid, IL-2, IL-15, IL-12 and IL-21 is used to significantly improve the early activation level of the gamma delta T cells; in the dynamic expansion stage, the concentration of metformin is adjusted step by step to support the continuous and efficient proliferation of the cells; in the gradient maturation stage, the combination system of N-acetyl cysteine, TWS119 and DL-alpha tocopheryl acetate is introduced to help maintain the functional activity and antioxidant state of the cells in the later culture stage. The gamma delta T cells with a purity higher than 90% and an expansion multiple more than 100 times can be obtained by using the method to culture for 10 days, and the cells exhibit significant killing activity to tumor cells, are suitable for the adoptive immunotherapy of tumor and infectious diseases, and have good clinical application prospects.

This invention relates to the field of microorganisms or enzymes, and discloses a T-cell activation and expansion medium and its culture process. The culture process includes: inoculating isolated T cells into a basal medium containing IL-2 and culturing them; on the 4th day of culture, adding IL-7, IL-15, and a defined molar ratio of nicotinamide and N-acetyl-L-cysteine ​​to the system; calculating the metabolic quotient by monitoring changes in lactate and glucose concentrations in the supernatant; and triggering a feeding action based on the metabolic quotient. During the feeding stage, the supplementation ratio of nicotinamide and N-acetyl-L-cysteine ​​is adjusted according to the threshold of the metabolic quotient. This invention achieves a precise coupling between mitochondrial energy drive and oxidative damage quenching, thereby solving the technical contradiction of difficulty in balancing expansion efficiency and phenotypic maintenance, effectively blocking the late-stage metabolic exhaustion pathway and directionally enriching memory subpopulations.

The application discloses a culture medium for constructing a gastric cancer ovary metastasis microtumor model and application. The application relates to the technical field of biotechnology, and provides a culture medium for constructing a gastric cancer ovary metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, Follistatin, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotinamide, N2 additives, cholera toxin, B27, ITS-X, Y-27632, gastrin, prostaglandin E2, beta-estradiol, progesterone, Galunisertib and a basic culture medium. The culture success rate of the gastric cancer ovary metastasis microtumor model is effectively improved.

The application discloses a culture medium for constructing a liver cancer lung metastasis microtumor model and application thereof. The application relates to the technical field of biology, and provides a culture medium for constructing a liver cancer lung metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant proteins EGF, HGF, IL-2, IL-15, R-spondin1, CXCL12, recombinant human lung surfactant proteins SP-A, SP-D, forskolin, A83-01, N-acetyl-L-cysteine, nicotinamide, N2 additives, B27 and a basic culture medium. The culture success rate of the liver cancer lung metastasis microtumor model is effectively improved.

The application discloses an ex vivo perfusion preservative solution for improving the curative effect of liver transplantation. 1000 mL of the ex vivo perfusion preservative solution comprises the following components: 20-60 mg of oxamic acid, 2 g of sodium penicillin, 15000-20000 U of heparin, 20 mg of N-acetyl cysteine, and HTK liquid is supplemented to 1000 mL. The ex vivo perfusion preservative solution for improving the curative effect of normal liver and fatty degeneration liver transplantation can effectively improve the liver injury caused by I / R. Compared with the HTK liquid used at present, the ex vivo perfusion preservative solution has better protection effect, and has a good application prospect for the preservation of donor liver in liver transplantation surgery, especially the fatty degeneration liver.

The application discloses a kind of composite fluorescent visualization sensor and its preparation method and application, it is related to analytical detection technical field;Composite fluorescent visualization sensor is prepared by the following method: first, synthesis of sulfhydryl succinic acid modified cadmium telluride quantum dots and N-acetyl-L-cysteine modified cadmium telluride quantum dots, then porphyrin stock solution is mixed with N-acetyl-L-cysteine modified cadmium telluride quantum dots, namely the chiral nano porphyrin solution can be obtained, finally it is mixed with the sulfhydryl succinic acid modified cadmium telluride quantum dots synthesized in advance, and composite fluorescent visualization sensor can be prepared;The composite fluorescent visualization sensor of quantum dot-nano porphyrin prepared by the application has good selectivity to L / D- theanine and has high sensitivity and anti-interference performance, and can be used for visualizing precise analysis of L / D- theanine content, with the advantages of simple operation, high sensitivity, low cost and the like.

The application relates to the technical field of medicines, and particularly discloses a novel immune stimulant, a preparation method and application thereof. The immune stimulant comprises an alpha-mannose modified imidazoquinoline derivative, an N-acetyl cysteine modified cytosine-guanine dinucleotide, a glycyrrhetinic acid-polyethylene glycol derivative, a phytic acid-glycine oligomer, a medicinal excipient and water for injection, three modified active ingredients are bridged by the phytic acid-glycine oligomer as a bridging unit, and a ternary coordination complex is formed through dynamic non-covalent coordination. The preparation method comprises the following steps: after raw materials are pretreated, the active ingredients are premixed, dissolved and solvent is recovered, then dynamic coordination reaction, ultrafiltration purification and concentration are carried out, and finally, a finished product is obtained through double-cavity preparation forming. The immune stimulant can be used for preparing a tumor immunotherapy, an anti-infection treatment, an immune deficiency disease auxiliary treatment drug or a vaccine adjuvant, and has the advantages of high active ingredient stability, good in-vivo delivery efficiency and outstanding synergistic effect.

The application relates to the fields of biological medicine and high polymer materials, and discloses a sustained-release implant type biodegradable stent loaded with an antitumor active component, which is composed of polylactic acid-glycolic acid copolymer, doxorubicin hydrochloride, citric acid-terminated polyethylene glycol polymer, zinc acetate dihydrate and N-acetyl cysteine. The metastable complex structure is constructed by in-situ coordination reaction between the components, so that the rapid release of the water-soluble doxorubicin hydrochloride in the initial implantation stage is blocked. After implantation, N-acetyl cysteine is preferentially dissociated and released, so that the zinc ion coordination unsaturated sites are generated in the coordination network; with the hydrolysis of the polylactic acid-glycolic acid copolymer, the generated lactic acid molecules intervene in the above unsaturated sites to generate a competitive coordination reaction, so that the coordination skeleton is destroyed and the antitumor drug is released. The application realizes the synchronization of the drug release rate and the high polymer matrix degradation rate, and the acetate dissociated from the zinc acetate can effectively buffer the pH value of the degradation microenvironment.

A combination of N-acetyl-L-cysteine, selenium in the form of selenomethionine and melatonin, and a medical product or pharmaceutical composition comprising such combination, useful for the treatment of a variety of diseases and conditions is described. The combination of N-acetyl-L-cysteine, selenium in the form of selenomethionine and melatonin is also useful for cosmetic treatment of skin and as an antibacterial agent.