146 results about "Glutathione-SH" patented technology

The invention as disclosed herein provides pharmaneutical compositions and methods for treating, ameliorating, or preventing the symptoms of Parkinson's Disease. The pharmaneutical compositions of the invention contain in an effective amount a first and a second composition, the first composition comprises an effective amount of one or more phosphatidylcholine formulations and the second composition comprises an effective amount of one or more constituents comprising essential fatty acid supplements, trace minerals, butyrate, electrolytes, methylating agents, reduced glutathione, or a combination thereof, in a suitable carrier.

Methods of modulating, adjusting, and maintenance of the glutathione redox status of an individual, or cell, tissues, bodily fluids, or body compartments of the individual, are disclosed, as are compositions suitable for such modulating, adjusting, and maintenance. The modulation or adjustment is achieved by altering the amounts of reduced glutathione versus oxidized glutathione in the individual, or cells, tissues, bodily fluids, or body compartments of the individual. Modulation, adjustment, and maintenance of the redox status of an individual enables treatment, prevention or suppression of diseases or symptoms associated with diseases. These methods are achieved by using quinone derivatives.

The present invention relates to a method for analyzing residual agricultural chemicals which comprises the steps of acting a reduced glutathione as a reactive substrate and a glutathione transferase serving as a catalyst for the reaction on a carbofuran derivative or a methomyl derivative as a carbamate type agricultural chemical of a new series to thus derivatize the agricultural chemical into a substance having a high choline esterase-inhibitory activity; reacting the substances formed through the derivatization reaction with a choline esterase; and then detecting the presence of the agricultural chemical as the new series of carbamate type one included in a sample to be examined on the basis of the changes in the choline esterase activity thus detected. The method of the present invention may serve as a powerful tool for the detection of the residual agricultural chemicals in grains such as rice and the detection of the content of agricultural chemicals remaining in agricultural products such as vegetables and fruits.

The invention discloses a Chinese hamster ovary culture medium as well as a preparation method and application thereof. The culture medium comprises ascorbic acid, pyridoxine hydrochloride, vitamin B12, nicotinamide, riboflavin, thiamine chloride, choline chloride, folic acid, calcium pantothenate, sodium pyruvate, reduced glutathione, inositol, biotin, hypoxanthine, putrescine, lipoic acid, linoleic acid, thymine, glucose, HEPES (2-hydroxyethyl) buffer solution, dextran sulphate, Pluronic-F68, various amino acids and salts thereof and various inorganic salts. The culture medium has low cost, and the component does not contain animal origin ingredients, does not contain protein and has definite chemical ingredients. All ingredients are cooperated and blended, and the Chinese hamster ovary culture medium can satisfy the basic growth requirement of cells, and can effectively regulate, transfer and control the culture of cells so as to realize good high-density cell culture. Compared with the culture effect of the existing culture medium, the culture effect of culture medium disclosed in the invention is at least equivalent to even superior to the existing culture medium.

The invention relates to a Fenneropenaeus chinensis antimicrobial protein gene and the recombination expression and the application. It has the amino acid sequence of SEQ ID No.2 and basic group sequence of SEQ ID No.1 in sequence table. It selects the carrier of the antimicrobial protein pCR T7/NT TOPO TA and host bacterium BL21(DE3)pLysS that could high efficiently express recombination protein. Aimed at the feature of plural disulfide bonds, using the oxidation reduction system made of oxidized form of glutathione and glutathione in separating and purification and protein recombination process, the recombination protein with antimicrobial ability would be gained. The protein has strong inhibition to the growth of gram positivebacteria, and some inhibition to gram negativebacteria.

The invention discloses a reduced-type glutathione freeze dried injection and making technique, which is characterized by the following: loading 95.0%-105.0% reduced-type glutathione (C10H17N3O6S) to display white block and powder; testing nitrosoferric sodium cyanide to display red; keeping the consistent reserving time of main peak of sample solution as comparison sample solution with pH value at 5.0-6.5; making dried weight not over 5.0% and the content of relative material not more than 4%; limiting the content of bacterial endotoxin less than 0.125EU; making other items satisfy the standard.

The invention is a method of treatment of the symptoms related to inflammation that accompanies the release of Tumor Necrosis Factor-alpha in diseases such as viral infection such as those affecting the respiratory tract by providing systemic glutathione (reduced) by oral administration of glutathione (reduced) in a liposome encapsulation or by the intravenous administration of reduced glutathione. The administration of a therapeutically effective amount of oral liposomal glutathione (reduced) results in improvement of symptoms of disease induced by the release of TNF-α in infectious disease states such as respiratory and other viruses. The product is novel in that it is stable across the temperature ranges encountered in shipping and does not need to be refrigerated for storage. Compounds enhancing the effect of the liposomal glutathione as well as intravenous glutathione are contemplated such as Selenium.

The invention discloses a copper metal organic framework nanoparticle functionalized hydrogel, a preparation method and application thereof. The preparation method comprises the following steps: dissolving carboxymethyl chitosan, and adding reduced glutathione and N-hydroxysuccinimide which are mixed and dissolved in deionized water; adding 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride to prepare glutathione modified carboxymethyl chitosan; preparing copper metal organic framework nanoparticles; then mixing the copper metal organic framework nanoparticles with deionized water; and adding the glutathione modified carboxymethyl chitosan, acrylamide, polyethylene glycol diacrylate, polyethylene glycol diglycidyl ether and an initiator to prepare the copper metal organic framework functionalized hydrogel. According to the invention, the obtained hydrogel has the capabilities of slowly and controllably releasing copper ions, removing active oxygen of a wound surface, always keeping the wound surface free of hydrops and a wet 3D microenvironment, protecting the wound from external injury and promoting wound healing.

The invention discloses a bone defect repair material based on modified perovskite quantum dots and a preparation method of the bone defect repair material. The bone defect repair material comprises the following raw materials in percentage by mass: 20-30% of hydroxyapatite, 10-20% of octacalcium phosphate, 0.5-2% of bone morphogenetic protein, 1-5% of collagen, 0.5-2% of chondroitin sulfate, 3-5% of carboxymethyl chitosan and the balance of a modified perovskite quantum dot solution. The modified perovskite quantum dot/amino carbon quantum dot solution is prepared from the following reaction raw materials in parts by mass: 0.5 to 1.5 parts of perovskite quantum dots, 8 to 16 parts of dipalmitoyl phosphatidyl ethanolamine, 3 to 7 parts of acidic amino acid, 1 to 3 parts of reduced glutathione, 0.5 to 1 part of EDC.HCl, 20 to 30 parts of chloroform and 100 parts of deionized water. Perovskite quantum dots and amino carbon quantum dots are introduced into the bone defect repair material for the first time, stem cell transfection is mediated by quantum dot and stem cell behavior changes are monitored by utilizing a fluorescence microscopy imaging technology; meanwhile, the stem cells are induced to differentiate towards osteoblasts and chondroblasts, and proliferation and regeneration of the osteoblasts are stimulated.

The invention discloses a preparation method and application of a chitosan-reduced glutathione polymer. The preparation method comprises the following steps: firstly, swelling chitosan in dilute acid; secondly, adding de-ionized water for fully dissolving chitosan; thirdly, adding an active agent, namely 1-ethyl-3-(3-dimethylaminopropyl)carboimide hydrochloride, a protective agent, namely N-hydroxysuccinimide and a reaction monomer, namely reduced glutathione, into a reaction container, uniformly mixing, adjusting the pH value of a reaction system to be proper, and reacting at a certain reaction temperature to obtain a liquid-state polymerization product. When the liquid-state polymerization product is applied to treatment of Cr<6+> ions and Hg<2+> ions in a water body, a relatively good treatment effect is achieved. The preparation process is simple; the prepared product can be used in the industry of heavy metal wastewater treatment.

The invention discloses a preparation for preventing and controlling crop bacterial wilt and a preparation method thereof. The preparation consists of the following raw materials: brassinolide, magnesium sulfate, ferrous sulfate, boric acid, compound amino acid, alcohol and water. The preparation method comprises the following steps of: dissolving the brassinolide into the alcohol; adding ethyoxyl trisiloxane; mixing uniformly to obtain a solution; mixing the obtained solution with the magnesium sulfate, ferrous sulfate, boric acid and compound amino acid; and adding water for fixing a volume to obtain the preparation for preventing and controlling crop bacterial wilt. The preparation for preventing and controlling crop bacterial wilt can be used for promoting the synthesis of reduced glutathione, increasing the activity of resistance enzymes, activating the expression of resistance genes, enhancing the resistance of crops, preventing bacterial from invading crops, effectively suppressing the growth of ralstonia solanacearum, remarkably relieving the mobidity extent of bacterial wilt, relieving the bacterial wilt of plants by over 54 percent, and fundamentally reducing economic loss caused by crop morbidity.

Provided is a biomarker that enables easy and rapid detection of oxidative stress on a living organism and enables prevention of tissue damage or cell necrosis by drug administration, and which is a powerful marker for the study of toxicity and pharmacokinetics of various agents. Oxidative stress is determined by measuring blood concentration of ophthalmic acid, which is a substance that varies in blood depending on the variation of reduced glutathione (GSH) concentration in a biological sample with the use of an analyzer such as a capillary electrophoresis-mass spectrometer. Further, an anti-oxidative stress agent is screened by administering an anti-oxidative stress candidate agent to a non-human animal under oxidative stress conditions, measuring blood concentration of ophthalmic acid, and evaluating the degree of decrease in the ophthalmic acid concentration.

The invention relates to antioxidant peptide and a preparation method thereof. According to the preparation method, peanut meal enzymolysis liquid is obtained by utilizing peanut meal and self-made koji-forming enzymes and through enzymatic hydrolysis; then the peanut meal enzymolysis liquid is separated and purified by utilizing an ultrafiltration membrane, a gel filtration chromatograph and a reversed-phase high-performance liquid chromatograph, so as to obtain a new antioxidant peptide product; and the amino acid sequence of the new antioxidant peptide product is determined. The amino acid sequence of the product is Tyr-Gly-Ser, and the molecular weight of the product is 325.3Da; the product has very strong antioxidant effect, and the oxygen free radical absorption capability of the product is 4 times of that of reduced glutathione; and the antioxidant peptide has the characteristic of small molecular weight and can be directly absorbed by human bodies, so that the antioxidant peptide can be widely applied to the fields of foods, health care products, cosmetics, and the like.

The present invention provides expression systems for exogenous polypeptides wherein the polypeptide is expressed as a fusion protein together with clover yellow virus Nuclear Inclusion a (NIa), the NIa component serving to autolyze the fusion protein after expression. This system can be used to express a novel polypeptide which we have designated KM31-7 protein and which is capable of reducing dichloroindophenol and reduced glutathione. This polypeptide is useful in the treatment of disorders caused by oxidative stress.

The invention relates to a method for determining soil contamination condition through crop markers. The method is characterized by comprising the following steps: taking maize seedlings as a study crop; after the maize seedlings grow for 14d, taking down maize leaves to prepare a crude enzyme liquid; testing the activity of ascorbate peroxidase in the maize leaves, and measuring the content of reduced glutathione in the maize leaves; when the activity of the ascorbate peroxidase and the content of the reduced glutathione change remarkably, determining that the soil is contaminated. When the soil is contaminated, the cadmium concentration is greater than or equal to 0.3 mg/kg. The method is low in cost and simple in operation; responses to low- concentration contaminants are sensitive; the method can be comprehensively applied to soil pollution condition diagnosis and quality evaluation.

The invention discloses a cleaning liquid after chemical mechanical polishing and a preparation method thereof. The cleaning liquid comprises the following raw material components in mass fraction: 0.01%-25% of alkali, 0.01%-30% of alcohol amine, 0.001%-1% of an antioxidant, 0.01%-0.1% of polypeptide, 0.01%-0.1% of amino acid, 0.01%-10% of a corrosion inhibitor, 0.01%-10% of a chelating agent, 0.01%-5% of a surfactant, 28.9-89.9% of water, based on 100% in total, wherein the amino acid is a combination of histidine and cysteine, and the polypeptide is reduced glutathione (peptide A) and oxidized glutathione (peptide B). The cleaning liquid is stronger in cleaning ability, is lower in corrosion rate, is stronger in BTA removal ability, is better in stability, and can realize the effects of cleaning, corrosion inhibition and BTA removal at the same time.

The invention relates to a method for detecting reduced glutathione (GSH) and/or oxidized glutathione (GSSG) based on a surface enhanced Raman spectroscopy, and also relates to a kit for detecting GSHand/or GSSG. The detection method and kit are simple in operation, high in detection speed and high in sensitivity, and can be well used for determining the content of GSH and GSSG in an alkylating agent infected cell sample, thereby rapidly screening and detecting an alkylating agent.

The invention provides a nano-material for treating heavy metal ions in sewage, and a preparation method thereof. The preparation method comprises the following steps: taking titanium isopropoxide, boric acid, absolute ethyl alcohol, sodium dihydrogen phosphate, palygorskite powder and water as raw materials, carrying out a high temperature reaction, then adding gamma-mercaptopropyltrimethoxysilane and gamma-aminoethyl aminopropyltrimethoxysilane, carrying out thermal-insulation modification treatment on corn straw, adding corn straw ultrafine powder, inoculating with a candida utilis seed solution, uniformly scattering reduced glutathione, fermenting, and carrying out post-treatment to obtain the nano-material. The nano-material can adsorb multiple heavy metal ions in a short time and efficiently remove the heavy metal ions in sewage.

A method of treatment of cancer with a formulation of liposomally encapsulated glutathione, that is preferably used orally, increases the level of glutathione in tissues in order to prevent and reverse the metabolic changes in cells that results in the formation of the metabolic “fuel supply” that supports cancer cells, and without which the cells can die out. The method prevents the oxidative stress that damages normal support cells such as stromal fibroblast cells. By blocking the “fuel supply,” the invention can protect, prevent and reverse these cells from the steps of autophagy and mitophagy, that results in the cells decreasing the normal production of ATP for energy and using aerobic glycolysis for energy production. The use of oral liposomally encapsulated glutathione will maintain the presence and normal function of caveolin in fibroblast and other cells, thus preventing their conversion to autophagic tumor stromal cells.

The invention discloses a preparation method and application of reduced glutathione lipidosome, relates to a preparation method and application of lipidosome, and aims at solving the technical problem of low inclusion rate of the lipidosome prepared by the existing method. The method comprises the following steps: 1, weighing soya bean lecithin and fatty acid glycerides, dissolving into absolute ethyl alcohol, and heating in a water bath to prepare an oil phase; 2, weighing reduced glutathione and a surfactant to be dissolved into distilled water, and heating in the water bath to prepare a water phase; 3, mixing the prepared oil phase with the water phase, emulsifying, carrying out vacuum rotary evaporation to remove absolute ethyl alcohol, adding distilled water, pre-cooling after ice-bath, and then carrying out freeze drying to obtain lipidosome powder. The reduced glutathione lipidosome disclosed by the invention is applied to intravenous injection, oral medication, transdermal drug delivery, and a drug delivery system for the lung and eyes. The encapsulation efficiency of the reduced glutathione lipidosome disclosed by the invention is 68.15%+/-1.53%. The preparation method is applied to the field of preparation of the lipidosome.

The invention discloses a disulfiram-based glucan nanometer prodrug, and a preparation method and applications thereof. The disulfiram-based glucan nanometer prodrug is a disulfiram-based glucan nanometer prodrug with oxidation-reduction response characteristics, glucan is selected as a skeleton, and a drug is connected with a polymer skeleton through a disulfide bond. The invention provides a polymer drug delivery system with oxidation-reduction response. According to the nanometer prodrug, the disulfide bond can be reduced into sulfydryl by reduced glutathione in cancer cells, and a responsemechanism is provided, so that the structure of a carrier is changed, and the drug is quickly released. The nanometer prodrug shows good biological safety and a good cancer cell killing effect, and an intelligent drug delivery system is provided for tumor treatment.