34 results about "Lipoxygenase activity" patented technology

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.

Compositions for inhibiting lipoxygenase enzymes (e.g., human epithelial 12 / 15-lipoxygenase (12 / 15-LOX) are provided. Compositions according to certain embodiments include a first solvent, a second solvent and a lipoxygenase inhibitor. In certain instances, the lipoxygenase inhibitor is present in the composition (e.g., compositions formulated for intravenous administration) at a concentration of 30 mg / mL or greater. Methods for treating or preventing a lipoxygenase (e.g., human epithelial 12 / 15-lipoxygenase (12 / 15-LOX))-mediated disease or disorder are also provided.

The invention discloses a post-harvest castanea mollissima calcification prevention and control method based on inhibition of lipoxygenase activity by salicylic acid, and belongs to the technical field of agricultural product preservation. Research finds that salicylic acid can inhibit lipoxygenase-mediated fatty acid oxidation, change lipid metabolism of chestnut kernels, increase the content of unsaturated fatty acid in the chestnut kernels, reduce lipid peroxidation and maintain cell integrity, so that calcification after chestnut harvesting is delayed or inhibited. The water content of the Chinese chestnuts can be kept, and a new target and strategy are provided for research and development of a preservative for inhibiting calcification of the Chinese chestnuts after harvesting and improvement of the storage quality and shelf life of the Chinese chestnuts and other horticultural crops after harvesting.

The present application belongs to the technical field of tobacco essence preparation and biocatalysis application, and particularly relates to a tobacco essence prepared based on composite enzymes and a preparation process. The process first proportionally compounds pectinase, beta-glucosidase, lipase, lipoxygenase, glucose oxidase and acetyltransferase, and forms microcapsules through a sodium alginate-chitosan system; prepares an extract after steam explosion pretreatment of tobacco powder, loquat leaves and monk fruit; adds the microcapsule enzymes for three-stage segmented enzymolysis; further adds hydroxypropyl-beta-cyclodextrin to include aroma molecules, and after enzyme inactivation and filtration, concentrates and adds vitamin E to stabilize the quality of the essence, so as to finally obtain a tobacco essence with rich aroma levels and good thermal stability. Through the synergistic effect of composite enzymes and multi-stage reaction regulation, the present application realizes the directional release and construction of flavor substances in natural raw materials, and the obtained essence is suitable for various application scenarios such as cigarette flavoring and paper spraying.

The application discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. The lipoxygenase mutant disclosed by the application has the following mutations on the basis of the amino acid sequence shown in SEQ ID NO. 1: H58E / I149K / A323G / R356S / S391G, D82I, D82V, S136V, S136A, I149R, I149K, A323G, R356S, A369G, N372R, V380W, S391G, S391A, S445G, S445A, D82I / I149K, D82I / S136V, H58E or H58D. The lipoxygenase mutant provided by the application has obviously improved enzyme activity and stability in catalyzing reactions, and improves the feasibility of industrial application.

This invention discloses a method for pretreating camellia seeds using low-temperature plasma, relating to the field of bioengineering technology. The method involves discharging the entire surface of the camellia seed using DBD, JET, DF, or RF at atmospheric pressure, preferably DBD 120kV×1min or RF 3m / min at a distance of 0-3mm for 20 passes. This achieves microporousization of the seed coat and targeted disruption of the cell wall to promote mass transfer. The treatment reduces lipase and lipoxygenase activities by approximately 31.72% and 35.69% (or 29.54%), respectively, and significantly enhances the antioxidant capacity of total phenols and DPPH / ABTS / FRAP in the methanol extract (up to 1.58 times the total phenols). This method simultaneously promotes the release of active ingredients, inhibits lipid oxidation, and improves oil yield and quality under low-temperature, non-thermal conditions, making it suitable for continuous industrial applications.

Methods and compositions are provided for enhancing the accumulation and / or recovery of recombinant casein expressed in soy. Endogenous Casein-Processing Interfering Proteins (CPIPs), such as oxidases like lipoxygenases (LOX), are present in the recombinant protein fractions and interfere with yield and / or purity. RNA interference (RNAi) mechanisms are leveraged in plasmids that knockdown genes expressing CPIPs and / or genes in the Soybean Vesicular Trafficking Pathway (SVTP). Knocking down of two or more target genes is manifested using a dual gene targeting approach, often via a single expression cassette containing multiple distinct shRNA constructs for enhanced efficiency. The shRNA constructs invoke a RNAi mechanism that leads to increased casein accumulation either: (1) directly, by suppressing CPIPs (e.g., LOX) to enable more efficient purification; or (2) indirectly, by modifying SVTP genes to route casein away from lytic vacuoles, preventing degradation.

Provided are a peroxide-containing liposome, a preparation method therefor, and use thereof. The peroxide-containing liposome is a spherical vesicle having a double-layer membrane structure and formed by lipid molecules containing one or more peroxy groups, and the lipid molecules contain carbon-carbon double bonds. The peroxide-containing liposome can be obtained by mixing peroxide-containing lipid molecules with an aqueous system, wherein the peroxide-containing lipid molecules are obtained by reacting unsaturated lipid molecules containing -CH=CH- and / or -C≡C- with an additive in the presence of oxygen and a solvent, and the additive is selected from one or more of lipoxygenase, an oxidation catalyst, and an oxidizing agent. By means of in-vitro experiments in cells and in-vivo experiments in animals, it is found that the peroxide-containing liposome can kill tumor cells and can be used as a sensitizing agent in combination with other tumor treatment methods to effectively improve the therapeutic effect on tumors, thus having good application prospects in resisting tumors.

The invention discloses a catalytic infrared pretreatment method for improving soybean oil quality by inactivating lipoxygenase, and relates to the technical field of agricultural product and food processing. The method comprises the following steps: cleaning and removing impurities from an air-dried soybean raw material, irradiating the soybean raw material under catalytic infrared rays (the power density is 7.89 kW and the wavelength is 4-18 microns), and controlling the temperature of the infrared rays irradiated to the surface of the soybean raw material to be 90-130 DEG C and the treatment time to be 3-9 minutes, so as to obtain a pretreated soybean raw material; squeezing oil by using a screw oil press to obtain virgin soybean crude oil; removing impurities through physical filtration to obtain pure soybean oil; through the innovative design of catalytic infrared pretreatment and parameter optimization, the inactivation rate of lipoxygenase is as high as 96.16%, the oil yield is as high as 16.67%, 0.97% and 11.99 mg / mL, the content of sterol and polyphenol is as high as 16.67%, 0.97% and 11.99 mg / mL, all indexes are far better than those of traditional hot air heating and microwave heating treatment, and the grease quality and functional component protection are remarkably improved.

The invention discloses a method for preparing hypoallergenic peanut milk by adding krill oil. The method comprises the following steps: (1) cleaning high-quality peanuts, and soaking the cleaned high-quality peanuts in water for 10 hours; (2) mixing the soaked peanuts with water, adding the krill oil into the mixture, and grinding into thick liquid; (3) performing high-pressure homogenization on the mixture obtained by grinding in the step (2) at 150MPa; (4) stirring the mixture subjected to high-pressure homogenization in the step (3) for 4 hours; 5) separating the mixture stirred in the step 4), and removing peanut residues to obtain peanut milk; (6) cooking the peanut milk obtained in the step (5), and cooling; 7, vitamin E is added into the cooled peanut milk obtained in the step 6, and nitrogen filling preservation is carried out. Krill oil-peanut oil milk fat film reconstruction is carried out through the high-pressure homogenization technology, it can be avoided that arachidonic acid and lipoxygenase generate proinflammatory substances, and the efficiency that EPA and DHA in krill oil phospholipid react with lipoxygenase to generate specific proinflammatory fading media can be improved.

The application discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. The lipoxygenase mutant disclosed by the application has the following mutation: S391G or S391A on the basis of the amino acid sequence shown in SEQ ID NO. 1. The lipoxygenase mutant provided by the application has obviously improved enzyme activity in catalyzing reaction, and improves the feasibility of industrial application.

The invention discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. According to the lipoxygenase mutant disclosed by the invention, on the basis of an amino acid sequence as shown in SEQ ID NO.1, the following mutations exist: H58E / I149K / A323G / R356S / S391G, D82I, D82V, S136V, S136A, I149R, I149K, A323G, R356S, A369G, N372R, V380W, S391G, S391A, S445G, S445A, D82I / I149K, D82I / S136V, H58E or H58D. The enzymatic activity and stability of catalytic reaction of the lipoxygenase mutant provided by the invention are obviously improved, and the feasibility of industrial application is improved.

The invention provides a strain of Yarrowia guilliermondii and application thereof. The preservation number of the Meyerozyma guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No. 34234, and the preservation number of the Meyerozyma guilliermondii is CGMCC No. 34234. The strain can secrete protease and lipoxygenase, and compared with a control group fermented by sterile water, the Yarrowia guilliermondii fermentation group can significantly increase the content of carotenoid degradation products, Maillard reaction products, chlorophyll degradation products, terpenoids and the like of tobacco leaves by applying the Yarrowia guilliermondii to enhanced fermentation of the tobacco leaves. Meanwhile, the aroma amount, aroma richness, maturity, sweetness and combustibility of the cigar tobacco leaves are remarkably improved, irritation and offensive odor are reduced, and the aroma characteristics of resin aroma, costustoot, nut aroma and the like are highlighted.

The invention belongs to the technical field of biological medicines, and particularly discloses an application of cumin in the aspect of neuroinflammation resistance, which is specifically as follows: lipopolysaccharide with the final concentration of 1 mu g / mL is used for inducing BV2 cells to form an acute neuroinflammation model, and cumin with gradient concentration is respectively added for treatment. Results show that 50-200 [mu] M cumin can significantly down-regulate expression of inflammatory factor nitric oxide, tumor necrosis factor-alpha and interleukin-6 secreted by lipopolysaccharide-induced BV2 cells. An immunoblotting method is used for discovering that cumin aldehyde can reduce expression of neuroinflammation 15-lipoxygenase / inflammasome signal channel targets in a dose-dependent manner, and meanwhile inflammatory reaction can be relieved by activating an oxidative stress signal channel. The results show that cumin has an anti-neuroinflammation effect. The cumin has a good application prospect for developing drugs or anti-inflammatory health-care products, cosmetics and food additives for treating inflammatory diseases by using cumin.

The invention provides a preparation process of yellow chickpea sprout peptide, the sprout peptide and application. The germinated peptide prepared by the invention is obtained by germinating yellow chickpeas to 0.8-1.2 cm and then hydrolyzing the germinated yellow chickpeas with a composite biological enzyme. The yellow chickpeas have the characteristics that 1, pretreatment is adopted before germination so as to inhibit the activity of lipoxygenase; 2, in the germination stage, the germination temperature of gradient heating is adopted; 3, a crushing mode of colloid mill treatment is adopted; and 4, carrying out ultrafiltration to intercept 1kDa, wherein the pH value of the final product is 5.5-6.0. The pretreated yellow chickpeas have the most abundant nutritional ingredients and the most vigorous life vitality in the bean germination stage, in the germination stage, the protein of the yellow chickpeas is activated by bioactive enzymes, various biological functions of life bodies are shown, and the germinated beans in the stage are the best choice as a protein raw material for preparing the bioactive peptide. Experiments prove that the composition has the efficacy of assisting in protecting gastrointestinal mucosal injury.

The application discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. The lipoxygenase mutant disclosed by the application has the following mutations on the basis of the amino acid sequence shown in SEQ ID NO. 1: I149R, I149K or D82I / I149K. The lipoxygenase mutant provided by the application has significantly improved enzyme activity and stability in catalyzing reactions, and improves the feasibility of industrial application.

Molecules for inhibiting arachidonate 15-lipoxygenase (ALOX-15) gene products including dsRNA (dsRNA) agents such as small interfering RNAs (siRNAs) for therapeutic use, additionally, methods to inhibit the expression of a target gene by administering these agents for the treatment of diseases involving ALOX-15 gene products.

The invention discloses mung bean plant milk with low beany flavor, low bitter taste and high stability and a preparation method of the mung bean plant milk, belongs to the technical field of processing of vegetable protein beverages, and aims to solve the technical problems that existing mung bean beverages are obvious in beany flavor, bitter and astringent in taste, easy to precipitate or flocculate during storage and the like. The specific preparation method comprises the following steps: firstly, carrying out heat treatment on a mung bean raw material to passivate lipoxygenase; pulping the treated raw materials, and sequentially carrying out step-by-step enzymolysis by adopting neutral protease, alpha-amylase, saccharifying enzyme and cellulase; and finally, carrying out high-pressure homogenization on the enzymatic hydrolysate. The prepared mung bean plant milk has the characteristics of low beany flavor, low bitter taste, high stability and the like, is light in flavor and smooth in mouth feel, and can realize long-term stability of a system under the condition of not additionally adding an emulsifying agent and a thickening agent.

The invention discloses an anti-inflammatory soothing scalp care composition for improving a scalp microenvironment. The anti-inflammatory soothing scalp care composition comprises any one or more of rhamnetin, diosmetin, quercitrin and kaempferol as functional active ingredients. The anti-inflammatory soothing scalp care composition provided by the invention can regulate the levels of arachidonic acid lipoxygenase-5, arachidonic acid lipoxygenase-12 and arachidonic acid lipoxygenase-15, can reduce the levels of inflammatory factors IL-6, IL-8 and TNF-alpha, and has the effects of regulating the scalp microenvironment, relieving scalp inflammation and soothing scalp.

The application discloses a preparation method of whole wheat flour with controlled lipase and lipoxygenase activity, and relates to the technical field of whole wheat flour processing and storage. The method comprises the following steps: adopting DBD plasma to treat the bran of wheat grains, and then directly grinding the wheat grains to obtain the whole wheat flour. The method controls the lipase and lipoxygenase in the bran of the wheat grains, thereby stabilizing the whole wheat flour, prolonging the storage period of the whole wheat flour, and improving the nutritional quality and processing quality of the whole wheat flour. The DBD plasma treatment is performed at a power of 300-500 W for 60-180 s.

The invention relates to the technical field of enzyme extraction and purification, in particular to an extraction method of lipoxygenase and peroxidase for millet. The preparation method comprises the following steps: S1, pretreating raw materials to obtain degreased millet flour; s2, performing low-pH one-step extraction on the degreased millet flour to obtain a crude enzyme solution; s3, adding ammonium sulfate into the crude enzyme solution to obtain a concentrated enzyme solution; s4, performing one-step SP cation exchange chromatography purification on the concentrated enzyme solution to obtain electrophoretic pure peroxidase and electrophoretic pure lipoxygenase; and S5, verifying the electrophoretic pure peroxidase and the electrophoretic pure lipoxygenase, and determining enzyme activity and specific activity. According to the method for extracting lipoxygenase and peroxidase for millet, LOX and POD of millet can be purified at a time under the condition that the pH value is in a low-acid condition through improvement of the method, the complexity that pH value adjustment, multiple extraction and multiple and multi-step chromatography are needed in a traditional process is avoided, the process is shortened, and the method is simplified.

The application discloses a peroxide-containing liposome and a preparation method and application thereof. The peroxide-containing liposome is a spherical vesicle with a double-membrane structure formed by lipid molecules containing one or more peroxide groups, and the lipid molecules contain carbon-carbon double bonds. The peroxide-containing liposome can be obtained by mixing and treating peroxide-containing lipid molecules with an aqueous system, wherein the peroxide-containing lipid molecules are obtained by reacting unsaturated lipid molecules containing-CH=CH- and / or-C=C- with an additive in the presence of oxygen and a solvent, and the additive is selected from one or more of lipoxygenase, an oxidation catalyst and an oxidant. Through in-vitro cell and in-vivo animal experiments, it is found that the peroxide-containing liposome can kill tumor cells, can be used as a sensitizer and cooperated with other tumor treatment methods, effectively improves the treatment effect on tumors, and has a good application prospect in the aspect of anti-tumor.

The invention discloses application of a traditional Chinese medicine composition in preparation of a medicine for reversing pancreatic cancer radiotherapy resistance by destroying redox balance and activating a ferroptosis procedure, and belongs to the technical field of medicine. Based on a new mechanism of pancreatic cancer radiotherapy resistance, i.e., tumor cells obtain resistance by up-regulating glutathione S-transferase P1 (GSTP1) to inhibit 5-lipoxygenase (ALOX5)-mediated ferroptosis, the invention provides a new application of a classic prescription dark plum pill or pharmaceutically acceptable extracts, effective parts or effective components thereof. According to the traditional Chinese medicine composition, by targeting a GSTP1 / ALOX5 axis, redox balance reconstructed in radiotherapy resistant cells is destroyed, lipid peroxidation and ferroptosis are reactivated, and therefore the sensitivity of tumor cells to radiotherapy is recovered. The radiotherapy sensitization effect and the mechanism of the dark plum pills are confirmed through systematic network pharmacology, bioinformatics analysis and in-vivo and in-vitro experiments, a brand new target spot and a treatment strategy are provided for overcoming pancreatic cancer radiotherapy resistance, and a scientific foundation is laid for modern development and clinical application of the dark plum pills.

The invention discloses application of GmLOX28 protein in improving drought resistance or salt resistance of soybeans, and belongs to the technical field of plant stress resistance. The invention aims to improve the drought resistance or salt resistance of soybeans. The invention provides application of a GmLOX28 protein in improving drought resistance and salt resistance of soybeans and improving antioxidant enzyme activity or lipoxygenase activity. The amino acid sequence of the GmLOX28 protein is shown as SEQ ID NO.3. The invention further provides a preparation method of the GmLOX28 protein. And a theoretical basis is provided for drought resistance or salt resistance of soybeans.

The application discloses a tobacco lipoxygenase (LOX2) gene and application thereof in cold-resistant breeding, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the LOX2 gene is shown as SEQ ID NO. 1. By constructing a recombinant expression vector containing the LOX2 gene and introducing the recombinant expression vector into tobacco, a transgenic tobacco material with overexpression of the LOX2 gene can be obtained. Compared with a control, the transgenic material shows stronger cold stress tolerance under a low-temperature treatment condition at 4 DEG C, and the contents of active oxygen, hydrogen peroxide and malondialdehyde are reduced, and the contents of jasmonic acid and methyl jasmonate are increased, and the expression of ADH and / or OPR1 genes is up-regulated. The LOX2 gene can be used for improving the cold resistance of tobacco and cultivating a new cold-resistant tobacco variety.

The present invention provides methods of treating conditions associated with cone photoreceptor degeneration, wherein the treatment comprises reducing or inhibiting lipid peroxidation in a cone photoreceptor. In particular, the present invention provides compounds for use in such methods, preferably metro death inhibitors.

The application discloses a lipoxygenase gene CoLOX4 and application thereof in improving the content of oleic acid in oil-tea camellia oil. The CoLOX4 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. The application constructs an overexpression vector and a virus-induced gene silencing (VIGS) vector of CoLOX4, and transiently transforms tobacco leaves, oil-tea camellia leaves and oil-tea camellia kernels respectively. The analysis of the content of fatty acid components shows that in the two kinds of receptors of oil-tea camellia leaves and oil-tea camellia kernels, silencing CoLOX4 can significantly improve the content of oleic acid, and in the three kinds of receptors of tobacco leaves, oil-tea camellia leaves and oil-tea camellia kernels, overexpression of CoLOX4 can significantly reduce the content of oleic acid. Therefore, the content of oleic acid in oil-tea camellia oil can be improved in a directional manner by inhibiting the expression of CoLOX4. The application provides a gene resource and an operation target for the molecular breeding of high-oleic-acid oil-tea camellia, and can be applied to the regulation of the content of oleic acid in oil plants.

The present disclosure provides compounds, compositions, and uses thereof, e.g., in the treatment of 12-lipoxygenase (12-LOX) mediated diseases.

The invention relates to the technical field of cigar tobacco processing. The invention provides a method for reducing green content of tobacco leaves by spraying lipoxygenase in an airing process and application of the method, and the lipoxygenase is sprayed on the front side and the back side of the tobacco leaves 2-4 days after the tobacco leaves wither. By analyzing the change influence of parameters such as pigment content, physical characteristics and appearance quality of the aired tobacco leaves sprayed with lipoxygenase, the result shows that the degradation of pigment of the tobacco leaves can be promoted, the appearance quality of the tobacco leaves can be improved, the tension of the tobacco leaves is enhanced, and the thickness, weight and the like of the leaves are reduced by spraying the lipoxygenase in the airing period. Wherein the effect of 400 U / g treatment (T2) is optimal, and the contents of chlorophyll a, chlorophyll b and carotenoid treated by 400 U / g after airing are respectively reduced by 19.35%, 12.27% and 11.72% compared with CK.

The application provides a lipid-type substrate GPX activity detection kit, a detection method and application, and the detection kit comprises the following reagents: AEA-M, dodecyl glucopyranoside, cholestanol hemisuccinate trimethylolamine salt, type 1 lipoxygenase and 2,3-naphthyl dialdehyde, wherein the AEA-M is synthesized by arachidonic acid and 1-amino-2,5-anhydro-1-deoxy-D-mannitol through an enzyme method. The application is suitable for detecting the activity of a lipid-type substrate GPX enzyme, the reagent reaction system has strong anti-turbidity capacity, the substrate has good solubility and good low-temperature stability, and is suitable for cell samples, high-fat content tissue samples and the like.