110 results about "Lipoxygenase activity" patented technology

The present invention provides a novel composition of matter comprised of a mixture of two specific classes of compounds—Free-B-ring flavonoids and flavans—for use in the prevention and treatment of diseases and conditions mediated by the COX-2 and 5-LO pathways. The present invention further provides a novel method for simultaneously inhibiting the cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LO) enzymes, and reducing cox-2 mRNA production. Finally, the present invention includes a method for weight loss and blood glucose control. The methods of this invention are comprised of administering to a host in need thereof an effective amount of the composition of this invention together with a pharmaceutically acceptable carrier. This invention relates generally to the prevention and treatment of diseases and conditions mediated by the cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LO) pathways, including but not limited to the relief joint discomfort and pain associated with conditions such as osteoarthritis, rheumatoid arthritis, and other injuries that result from overuse.

A stabilized bran component and a stabilized whole grain wheat flour containing the component are produced by subjecting a bran-enriched coarse fraction which contains bran, germ and starch to grinding or milling to reduce grittiness of the coarse fraction without substantially damaging the starch due to abrasion. The coarse fraction may be stabilized by heating to substantially reduce the lipase and lipoxygenase activity of the coarse fraction without substantial gelatinization of the starch. High levels of natural antioxidants and vitamins are retained while avoiding substantial acrylamide formation during the stabilization. The stabilized coarse fraction or stabilized bran component may be combined with a fine fraction which contains predominantly endosperm to obtain a stabilized whole grain wheat flour. The stabilized whole grain wheat flours and the stabilized bran component exhibit extended shelf life and may be used for making baked goods, such as cookies, with desirable oven spread, and a non-gritty mouthfeel.

A red blood cell storage composition includes a composition of red blood cells and biochemistry altering reagents, the biochemistry altering reagents being present at a concentration so as to reduce the percent hemolysis of the red blood cells during the freeze-thaw cycle below that of the percent hemolysis of the red blood cells in the absence the biochemistry altering reagents. The red blood cell storage composition preferably includes reagents selected from: modifiers of glycolytic / metabolic components, modifiers of antioxidant potential, effectors of intracellular ionic distribution, modifiers of membrane fluidity, modifiers of cytoskeletal structure, effectors of the cyclooxygenase second messenger pathway, effectors of the lipoxygenase second messenger pathway, effectors of the hexose monophosphate second messenger pathway, effectors of the phosphorylation second messenger pathway, modifiers of specific messenger molecules, and combinations thereof.

The present invention provides, inter alia, compounds having the structure (1) compositions containing such compounds are also provided. Methods for using such compounds or compositions for treating or ameliorating the effects of a cancer having a cell that harbors an oncogenic RAS mutation, for modulating a lipoxygenase in a ferroptosis cell death pathway, and for depleting reduced glutathione (GSH) in a cell harboring an oncogenic RAS mutation are further provided.

There is provided a compound of formula (I), wherein R1, R2, R3, X and Y have meanings given in the description, and pharmaceutically-acceptable salts thereof, which compounds are useful in the treatment of diseases in which inhibition of the activity of a lipoxygenase (e.g. 15-lipoxygenase) is desired and / or required, and particularly in the treatment of inflammation.

The invention provides a novel herbal composition containing the extracts of the leaves and / or stem of <italic>Argemone mexicana < / highlight>plant, optionally containing the extracts of the fruits of <italic>Cuminum cyminum< / highlight>, which exhibits useful in vitro, in vivo and interesting immunological and pharmacological activities; a process for preparation thereof; and a method of treatment of psoriasis and related immunological and biological disorders by administration of the said novel herbal composition. The useful in vitro, in vivo and interesting immunological and pharmacological activities exhibited by the extracts and fractions of the leaves and / or stem of <italic>Argemone mexicana < / highlight>plant include immunosuppression, lymphoproliferation inhibition, cytokine modulation such as IL-2 inhibition, IFNgamma inhibition, IL-10 induction, keratinocyte proliferation inhibition, keratolytic activity, endothelial cell proliferation inhibition, inhibition of cell adhesion molecule expression such as ICAM-1, MEST inhibition, and enzymes inhibition such as p60src Tyrosine kinase, which are known to be involved in anti-psoriatic activity. The novel herbal composition(s) is useful in the treatment of various disorders, such as psoriasis including plaque psoriasis, gutatte psoriasis, pustular psoriasis and psoriasis of the nails; dermatitis and scleroderma; eczema; inflammatory disorders and other autoimmune diseases like psoriatic arthritis, rheumatoid arthritis, Crohn's disease, multiple sclerosis, irritable bowel disease, ankylosing spondilitis, systemic lupus erythremetosus and Sjogren's syndrome; allergies like asthma and chronic obstructive pulmonary disease and is safe, well-tolerated, non-toxic, with minimal and reversible adverse reactions or side effects, and most importantly, with minimal relapse or recurrence of the disease following completion of a treatment regimen. The invention also describes the presence of phosphodiesterase (III, IV and V) inhibition and 5-Lipoxygenase inhibition in the aqueous, ethanolic or aqueous-ethanolic extracts of fruits of <italic>Cuminum cyminum < / highlight>plant.

The present invention provides novel mechanisms that regulate the production of anti-inflammatory and pro-inflammatory mediators generated by 5-lipoxygenase. In this regard, the present invention establishes that phosphorylation of 5-Lipoxygenase by protein kinase A, has a crucial role in determining the end products of 5-Lipoxygenase. With translocation to the nucleus, potent proinflammatory leukotrienes are produced, whereas following phosphorylation by protein kinase A, anti-inflammatory mediators are produced. The present invention also discloses compounds that regulate these pro- and anti-inflammatory mediators.

A pharmaceutical formulation is provided which is useful as a leukotrine synthesis and IL4 inhibitor and as a Th1 immunomodulator. The formulation comprises an effective amount of a combination of extracts and lyophilized extracts of Piper betel and Murrya koeniggi. The formulation can be formulated with pharmaceutically acceptable carriers. The formulation can be used in a method of treating humans for respiratory conditions.

This invention relates to an herbal composition for the treatment and remedy of bronchial respiratory difficulties, more particularly this invention describes the process of separation, physicochemical characterization and biological response evaluation of active components obtained from extracts of any plant parts including leaves, barks, roots and seeds of plants M. koenigii and P. betle in order to establish their role on the treatment and remedy of bronchial respiratory troubles.

In accordance with the present invention, there are provided novel compounds that have a variety of properties, i.e., antioxidant, anti-inflammatory, antiviral, antibacterial, and antifungal properties. Invention compounds, therefore, have the ability to impart a variety of beneficial physiological effects, e.g., to protect neurons and / or to promote neuroregeneration and / or to promote memory formation and / or to act as protein phosphatase or kinase inhibitors and / or to act as lipoxygenase inhibitors. Such compounds are useful for treatment of a variety of indications, including neurodegenerative diseases and conditions, diabetes, ischemia associated with heart disease, and memory deficit. In another aspect of the present invention, there are also provided formulations containing one or more of the above-described compounds, optionally further containing additional neurologically active compound(s) and / or adjuvants to facilitate delivery thereof across the blood / brain barrier. In still another aspect of the present invention, there are further provided methods for treating a wide variety of neurological indications, e.g., acute neural injuries, chronic injuries, promoting memory formation, and the like.

Correlations between polymorphisms in the 5-lipoxygenase gene, or polymorphisms in the leukotriene C4 synthase gene, and a subject's phenotypic response to treatment with a leukotriene receptor antagonist for respiratory disease are described. Methods of screening subjects to aid in treatment, and methods of screening therapeutic compounds, are presented.

The present inventors found that the natural flavor intrinsic to soybean is retained more than ever before when soybean is processed to prepare a processed soybean material having a specific lipid content, a dietary fiber content, an acid-soluble component content and a protein content, whereby the present problems were solved. More specifically, the processed soybean material of the present invention is such that, on a solid weight basis, (1) a content of lipid, extracted with a mixed organic solvent wherein a ratio of chloroform to methanol is 2:1, is 10 to 40% by weight, (2) a dietary fiber content is 5 to 40% by weight, (3) a potassium content is 1% by weight or less, (4) a protein content is 30 to 65% by weight, and (5) a sum of γ-conglycinin and 24 kDa protein is 2.4 times or less of lipoxygenase protein in soybean proteins.

The use of compounds of the formula (I) Ar1-L1-Ar2-L2-C(R3)(R4)N(OR1)C(═Y)—R2 (I) where Y is selected from O or S; R1 is H, a salt or readily hydrolysable substituent; R2 is selected from H or CH3, CH2F, CF2H or CF3; R3 and R4 are selected independently from H, C 1-4 alkyl or alkenyl, CF3, CH2F, CF2H and F, with the proviso that if either R3 or R4 is H, then the other is not H; L1 is a linker group; L2 is a linker group comprising an optionally substituted or unsubstituted unsaturated branched or straight chain alkyl group; Ar1 is an optionally substituted or unsubstituted aryl or heterocyclic group; and Ar2 is an optionally substituted or unsubstituted aryl or heterocyclic group, in the treatment of 5-lipoxygenase mediated cancer provide improved therapies due to the effective inhibition of 5-lipoxygenase and long duration of activity in vivo after oral administration.

There is provided compounds of formula I,wherein R1, R2, X1, X2 and n have meanings given in the description, and pharmaceutically-acceptable salts thereof, which compounds are useful in the treatment of diseases in which inhibition of the activity of a lipoxygenase (e.g. 15-lipoxygenase) is desired and / or required, and particularly in the treatment of inflammation.

The present invention provides oxazolyl and thiazolyl inhibitors of 15-LO, pharmaceutical compositions containing such inhibitors and methods for treating diseases related to the 15-LO cascade using such compounds and compositions.

Disclosed are compositions which include an isolated soft tissue from Modiolus modiolus (“MM”) and compositions which include an oil isolated from MM or a portion thereof. Also disclosed are methods of treating adenocarcinoma in a subject; methods of decreasing proliferation of adenocarcinoma cells, or of inducing apoptosis of adenocarcinoma cells, or of inducing differentiation of adenocarcinoma cells into non-cancerous cells; methods of inhibiting the activity of 5-lipoxygenase or 12-lipoxygenase or both in cells; and methods of treating a subject suffering from a disease or condition associated with excessive 5-lipoxygenase activity or 12-lipoxygenase activity or both. A process for preparing an MM extract by contacting MM or a portion thereof with a solvent under conditions effective to extract one or more materials from the MM or portion thereof is also described.

process is described for preparing a compound of Formula (1.3.0):comprising: establishing a reaction mixture consisting ofin an aprotic solvent; in the presence of a strong base in solid form selected from the group consisting of sodium hydroxide, NaOH; and potassium hydroxide, KOH;-and optionally-in the presence of a catalytic amount of cesium carbonate, Cs2CO3, or of a phase transfer catalyst, especially a quaternary ammonium salt or a phosphonium salt-followed by-heating said reaction mixture under a nitrogen atmosphere; whereby there is produced a compound of Formula (1.3.0); and in a preferred embodiment the aprotic solvent is DMSO, the strong base in solid form is NaOH in powder or pellet form, and the phase transfer catalyst is tetra-n-butylammonium chloride (TBAC), which is used to prepare a preferred compound, useful as a 5-lipoxygenase inhibitor, of the formula:especially the substantially pure methanesulfonate salt thereof.

The invention relates to a recombined lipoxygenase PhLOX and a preparation method thereof. The PhLOX has activities of a lipoxygenase, a hydroperoxide lyase and a propadiene oxide synthetase at the same time. The method includes following steps: extracting total RNA from porphyra haitanensis; designing a primer according to a gene sequence of the lipoxygenase; amplifying an overall-length cDNA sequence through an RACE technology; recombining the overall-length cDNA sequence to an expression vector pET-28a; converting escherichia coli E.coli BL21; screening out positive clone; and generating the multifunctional lipoxygenase of a bacteria liquid with an expression quantity being 2mg / L through inducible expression. Compared with a lipoxygenase in the prior art, the recombined lipoxygenase has a plurality of enzyme functions, is free of a strict substrate specifity and allows downstream products in various structures to be formed.

The present invention relates to an extract isolated from Sophorae Radix useful for prevention and treatment of respiratory diseases. More specifically, the present invention relates to a pharmaceutical drug comprising the Sophorae Radix extract having excellent effects of inhibiting airway contraction, respiratory infections, 5-lipoxygenase activity, phosphodiesterase 4 activity, airway hyper-responsiveness and airway remodeling; antagonistic activity against leukotriene D4; and an antitussive effect, thus being useful for prevention and treatment of respiratory diseases such as asthma, acute or chronic bronchitis, allergic rhinitis, acute upper respiratory infections and acute lower respiratory infections, and the like.

The invention discloses a method for testing the activity of corn seed embryo lipoxidase. The method comprises the following steps of: reacting an enzyme activity detection system at temperature of 24 to 26 DEG C, testing outside dimension (OD) values of the system at a position of 234 nanometers after reaction for t1 and t2, and recording the values as OD(t1) and OD(t2); and calculating the activity of the lipoxidase of the corn seed embryo according to the following formula: A=60s*[OD(t2)-OD(t1)] / 0.01 / (t2-t1). The enzyme activity detection system consists of tween 20, linoleic acid, Tris-Hc1 buffer solution with pH value of 8.0 and concentration of 0.02 mol / ml and the corn seed embryo lipoxidase to be tested, wherein the concentration of the linoleic acid is 0.3mmol / L, the concentrationof tween 20 is 0.25mmol / L; and the concentration of the corn seed embryo lipoxidase to be tested is not more than 0.0167mg / 3ml. The enzyme activity is defined that: the enzyme amount required by the enzyme activity detection system for increasing 0.01 absorbance at every minute is an enzyme activity unit.

The invention discloses soybean blending edible oil and a preparation process thereof. The soybean blending edible oil is prepared from the following raw materials in parts by weight: 100 parts of soybean oil, 20-30 parts of olive oil, 15-25 parts of grape seed oil, 10-20 parts of rosehip oil, 10-15 parts sea buckthorn oil, 6-10 parts of perilla seed oil, 1-7 parts of corn germ oil, and 0.1-0.2 part of a vegetable oil modifying agent; the vegetable oil modifying agent is prepared from the following ingredients in parts by weight: 2-3 parts of glucose oxidase, 3-4 parts of o-phosphoethanolamine, 0.8-1.2 parts of alpha-tocopherol, 0.6-1 part of a rosemary extract, 1-2 parts of lycopene, 0 .3-0.5 part of catechin and 0.4-0.6 part of phytic acid. The preparation process comprises the followingsteps of preparing the vegetable oil modifying agent, preparing compound vegetable oil and preparing the soybean blending edible oil. According to the soybean blending edible oil, the vegetable oil modifying agent is utilized to passivate lipoxygenase activity, prevent the browning reaction of soybean blending oil, reduce the content of dissolved oxygen and improve the oxidation resistance and thermal stability of blending oil so as to enable the blending oil to maintain good flavor and quality.

Provided are: tofu-like food and soy milk containing fibrous material, each of which has good texture and flavor and enables simultaneous intake of almost all of nutritional components in soybeans, such as proteins, lipids, glucides, fibrous material, minerals, vitamin components, and isoflavone compounds. Also provided is a process for producing soy milk containing fibrous material, which comprises the steps of: (A) dissolving and dispersing dehulled soybean powdery material having a nitrogen solubility index of 75 to 90, a lipoxygenase value of 30 to 120, an n-hexanal content of 0.5 ppm to 2 ppm, and a peroxide value of 0.2 meq / kg to 3.0 meq / kg in water to obtain dispersion; (B) subjecting the dispersion to heat treatment at 120° C. to 160° C. for 1 to 600 seconds; and (C) after the step (B), cooling the dispersion to 100° C. or lower and subjecting the resultant dispersion to emulsification and dispersion treatment under a pressure of 25 MPa to 200 MPa to produce soy milk containing fibrous material.

The present invention provides a method for bleaching or whitening a dairy product, comprising adding a lipoxygenase (LOX) to the dairy product. The method of the invention may be used to whiten milk, cheese, butter oil, cream or whey products. The invention further provides the use of a LOX to bleach a dairy product and a dairy product whitened by the methods of the invention.

The present invention relates to a novel compound having a molecular formula C<subscript>13< / highlight>H<subscript>15< / highlight>NO<subscript>5 < / highlight>and a process for the isolation of said compound. The present invention also relates to a method of inhibiting 13-lipoxygenase and having free radical scavenging activity.

The invention belongs to the field of crop fertilizers, and particularly relates to a special fertilizer for enhancing the original aroma of apples. The special fertilizer contains ammonium nitrate, monopotassium phosphate, potassium sulfate, calcium humate, magnesium sulfate, sodium borate, fulvic acid iron, an amino acid and an aroma enhancing activating agent. The special fertilizer is characterized in that the nutrient contents of nitrogen, phosphorus and potassium are reasonably compounded according to the nutrition uptake characteristics of the apples during growth, medium trace elements: calcium, magnesium, boron and iron, which are needed during the young fruit period and the fruit swelling period of the apples are added in the special fertilizer, so that the growth and the development of the apples are promoted, and the yield of the apples is increased; besides, the amino acid and the aroma enhancing activating agent are added in the fertilizer, the amino acid is a substrate which is synthesized from the volatile aromatic esters of the apples, the aroma enhancing activating agent can promote the activity of transaminase and lipoxygenase in the apples and increase the contents of the aromatic esters and alcohols, so that the original aroma of the apples is stronger. When the special fertilizer is applied in the young fruit period and the fruit swelling period of the apples, not only the yield of the apples can be increased, but also the original aroma of the apples can be enhanced. The special fertilizer has a relatively high market application value.

There is provided compounds of formula (I) wherein W is an optionally substituted aryl or heteroaryl group, and pharmaceutically-acceptable salts thereof, which compounds are useful in the treatment of diseases in which inhibition of the activity of a lipoxygenase (e.g. 15-lipoxygenase) is desired and / or required, and particularly in the treatment of inflammation.

The invention discloses a method for suppressing soybean lipoxygenase activity by use of cyclodextrin, belonging to the technical field of cyclodextrin. According to the invention, the cyclodextrin is subjected to complexation with hydrophobic amino acid of the soybean lipoxygenase molecules so as to change the active conformation of lipoxygenase and suppress the activity of the lipoxygenase by 86.26% maximally. The method disclosed by the invention is simple and easy to implement, saves energy, has low cost, realizes good working environment and sanitary environment, and avoids pollution; and through the method, the deodorization technology for soybean and products thereof can be simplified.

The present invention provides a novel composition of matter comprised of a mixture of two specific classes of compounds --Free-B-Ring flavonoids and flavans-- for use in the prevention and treatment of diseases and conditions associated with mouth, gums and teeth. This composition of matter simultaneously inhibits cyclooxygenase (COX) and lipoxygenase (LOX) enzymatic activity and reduces cytokine production at the mRNA level in normal, aged and damaged periodontal cells and tissues. This invention further provides a method for the prevention and treatment of diseases and conditions of the mouth, gums and teeth. The method for preventing and treating diseases and conditions of the mouth, teeth and gums is comprised of administering to a host in need thereof a therapeutically effective amount of a composition comprising a mixture of Free-B-Ring flavonoids and flavans synthesized and / or isolated from a single plant or multiple plants, preferably in the Scutellaria, Oroxylum, Acacia or Uncaria genus of plants and pharmaceutically and / or cosmetically acceptable carriers. Finally the present invention provides a method for the prevention and treatment of diseases and conditions of the mouth, teeth or gums, including but not limited to periodontal diseases, such as gingivitis, periodontitis, pulpitis, periodontal conditions caused by the physical implantation of oral dentures, trauma, injuries, bruxism, neoplastic and other degenerative processes; material alba, pellicles, dental plagues, calculus, and stains. Use of the composition described herein also affords the benefit of maintaining optimum saliva production and pH, minimizing bacterial growth, reducing the formation of pellicles and plague, inhibiting tooth decalcification and tooth caries (decay), promoting remineralization, which yields healthy gums, whitening teeth, maintaining healthy oral hygiene and reducing oral malodour (halitosis).