48 results about "Lipoxygenase activity" patented technology

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.

Compositions for inhibiting lipoxygenase enzymes (e.g., human epithelial 12 / 15-lipoxygenase (12 / 15-LOX) are provided. Compositions according to certain embodiments include a first solvent, a second solvent and a lipoxygenase inhibitor. In certain instances, the lipoxygenase inhibitor is present in the composition (e.g., compositions formulated for intravenous administration) at a concentration of 30 mg / mL or greater. Methods for treating or preventing a lipoxygenase (e.g., human epithelial 12 / 15-lipoxygenase (12 / 15-LOX))-mediated disease or disorder are also provided.

The invention discloses a post-harvest castanea mollissima calcification prevention and control method based on inhibition of lipoxygenase activity by salicylic acid, and belongs to the technical field of agricultural product preservation. Research finds that salicylic acid can inhibit lipoxygenase-mediated fatty acid oxidation, change lipid metabolism of chestnut kernels, increase the content of unsaturated fatty acid in the chestnut kernels, reduce lipid peroxidation and maintain cell integrity, so that calcification after chestnut harvesting is delayed or inhibited. The water content of the Chinese chestnuts can be kept, and a new target and strategy are provided for research and development of a preservative for inhibiting calcification of the Chinese chestnuts after harvesting and improvement of the storage quality and shelf life of the Chinese chestnuts and other horticultural crops after harvesting.

The invention relates to tobacco lipoxygenase NtLOX2 capable of regulating and controlling pigment synthesis and application of the tobacco lipoxygenase NtLOX2. The amino acid sequence of the tobacco lipoxygenase NtLOX2 is shown as SEQ ID No.1. The invention provides tobacco lipoxygenase NtLOX2 which is found to be highly related to the content of tobacco pigment, and after the gene is silenced, the content of the pigment in tobacco is obviously increased.

The present application belongs to the technical field of tobacco essence preparation and biocatalysis application, and particularly relates to a tobacco essence prepared based on composite enzymes and a preparation process. The process first proportionally compounds pectinase, beta-glucosidase, lipase, lipoxygenase, glucose oxidase and acetyltransferase, and forms microcapsules through a sodium alginate-chitosan system; prepares an extract after steam explosion pretreatment of tobacco powder, loquat leaves and monk fruit; adds the microcapsule enzymes for three-stage segmented enzymolysis; further adds hydroxypropyl-beta-cyclodextrin to include aroma molecules, and after enzyme inactivation and filtration, concentrates and adds vitamin E to stabilize the quality of the essence, so as to finally obtain a tobacco essence with rich aroma levels and good thermal stability. Through the synergistic effect of composite enzymes and multi-stage reaction regulation, the present application realizes the directional release and construction of flavor substances in natural raw materials, and the obtained essence is suitable for various application scenarios such as cigarette flavoring and paper spraying.

The present invention discloses a Cslnc170-CsLOX4 gene pair in tea plants and their application in improving anthracnose resistance, belonging to the field of tea plant gene application technology. The gene is located on the chromosome of the tea genome. The Cslnc170 gene, with a total length of 1581bp, is located 9254bp downstream of the CsLOX4 gene, a member of the 13-lipoxygenase (13-LOX) family. Beneficial effects: The Cslnc170-CsLOX4 gene can be used to regulate tea plants' resistance to anthracnose. From the perspective of their mode of action, loop 4 (252bp-271bp) of the Cslnc170 secondary structure and the 930bp-952bp region of the CsLOX4 promoter are the main functional regions for their interaction. The present invention provides a new direction for improving tea plant disease resistance and has high application prospects and economic value.

The application discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. The lipoxygenase mutant disclosed by the application has the following mutations on the basis of the amino acid sequence shown in SEQ ID NO. 1: H58E / I149K / A323G / R356S / S391G, D82I, D82V, S136V, S136A, I149R, I149K, A323G, R356S, A369G, N372R, V380W, S391G, S391A, S445G, S445A, D82I / I149K, D82I / S136V, H58E or H58D. The lipoxygenase mutant provided by the application has obviously improved enzyme activity and stability in catalyzing reactions, and improves the feasibility of industrial application.

This invention discloses a method for pretreating camellia seeds using low-temperature plasma, relating to the field of bioengineering technology. The method involves discharging the entire surface of the camellia seed using DBD, JET, DF, or RF at atmospheric pressure, preferably DBD 120kV×1min or RF 3m / min at a distance of 0-3mm for 20 passes. This achieves microporousization of the seed coat and targeted disruption of the cell wall to promote mass transfer. The treatment reduces lipase and lipoxygenase activities by approximately 31.72% and 35.69% (or 29.54%), respectively, and significantly enhances the antioxidant capacity of total phenols and DPPH / ABTS / FRAP in the methanol extract (up to 1.58 times the total phenols). This method simultaneously promotes the release of active ingredients, inhibits lipid oxidation, and improves oil yield and quality under low-temperature, non-thermal conditions, making it suitable for continuous industrial applications.

Methods and compositions are provided for enhancing the accumulation and / or recovery of recombinant casein expressed in soy. Endogenous Casein-Processing Interfering Proteins (CPIPs), such as oxidases like lipoxygenases (LOX), are present in the recombinant protein fractions and interfere with yield and / or purity. RNA interference (RNAi) mechanisms are leveraged in plasmids that knockdown genes expressing CPIPs and / or genes in the Soybean Vesicular Trafficking Pathway (SVTP). Knocking down of two or more target genes is manifested using a dual gene targeting approach, often via a single expression cassette containing multiple distinct shRNA constructs for enhanced efficiency. The shRNA constructs invoke a RNAi mechanism that leads to increased casein accumulation either: (1) directly, by suppressing CPIPs (e.g., LOX) to enable more efficient purification; or (2) indirectly, by modifying SVTP genes to route casein away from lytic vacuoles, preventing degradation.

Provided are a peroxide-containing liposome, a preparation method therefor, and use thereof. The peroxide-containing liposome is a spherical vesicle having a double-layer membrane structure and formed by lipid molecules containing one or more peroxy groups, and the lipid molecules contain carbon-carbon double bonds. The peroxide-containing liposome can be obtained by mixing peroxide-containing lipid molecules with an aqueous system, wherein the peroxide-containing lipid molecules are obtained by reacting unsaturated lipid molecules containing -CH=CH- and / or -C≡C- with an additive in the presence of oxygen and a solvent, and the additive is selected from one or more of lipoxygenase, an oxidation catalyst, and an oxidizing agent. By means of in-vitro experiments in cells and in-vivo experiments in animals, it is found that the peroxide-containing liposome can kill tumor cells and can be used as a sensitizing agent in combination with other tumor treatment methods to effectively improve the therapeutic effect on tumors, thus having good application prospects in resisting tumors.

The invention discloses a catalytic infrared pretreatment method for improving soybean oil quality by inactivating lipoxygenase, and relates to the technical field of agricultural product and food processing. The method comprises the following steps: cleaning and removing impurities from an air-dried soybean raw material, irradiating the soybean raw material under catalytic infrared rays (the power density is 7.89 kW and the wavelength is 4-18 microns), and controlling the temperature of the infrared rays irradiated to the surface of the soybean raw material to be 90-130 DEG C and the treatment time to be 3-9 minutes, so as to obtain a pretreated soybean raw material; squeezing oil by using a screw oil press to obtain virgin soybean crude oil; removing impurities through physical filtration to obtain pure soybean oil; through the innovative design of catalytic infrared pretreatment and parameter optimization, the inactivation rate of lipoxygenase is as high as 96.16%, the oil yield is as high as 16.67%, 0.97% and 11.99 mg / mL, the content of sterol and polyphenol is as high as 16.67%, 0.97% and 11.99 mg / mL, all indexes are far better than those of traditional hot air heating and microwave heating treatment, and the grease quality and functional component protection are remarkably improved.

The invention belongs to the technical field of plant genetic engineering, and relates to application of an LOX2 gene in reducing the content of tobacco pigment. According to the invention, a tobacco lipoxygenase 2 coding gene is obtained from tobacco by using a homologous cloning technology, and an overexpression vector of the gene is constructed and transformed into a tobacco plant to obtain the tobacco plant overexpressed by the LOX2 gene. Through detection of the LOX2 gene overexpression tobacco plant, the xanthophyll content of the transgenic plant is lower than 22% of a control group, the beta-carotene content of the transgenic plant is lower than 21% of the control group, the violaxanthin content of the transgenic plant is lower than 23% of the control group, and the neoxanthin content of the transgenic plant is lower than 20% of the control group. Therefore, the LOX2 gene has an important application prospect in the aspect of tobacco pigment degradation.

The invention discloses a method for preparing hypoallergenic peanut milk by adding krill oil. The method comprises the following steps: (1) cleaning high-quality peanuts, and soaking the cleaned high-quality peanuts in water for 10 hours; (2) mixing the soaked peanuts with water, adding the krill oil into the mixture, and grinding into thick liquid; (3) performing high-pressure homogenization on the mixture obtained by grinding in the step (2) at 150MPa; (4) stirring the mixture subjected to high-pressure homogenization in the step (3) for 4 hours; 5) separating the mixture stirred in the step 4), and removing peanut residues to obtain peanut milk; (6) cooking the peanut milk obtained in the step (5), and cooling; 7, vitamin E is added into the cooled peanut milk obtained in the step 6, and nitrogen filling preservation is carried out. Krill oil-peanut oil milk fat film reconstruction is carried out through the high-pressure homogenization technology, it can be avoided that arachidonic acid and lipoxygenase generate proinflammatory substances, and the efficiency that EPA and DHA in krill oil phospholipid react with lipoxygenase to generate specific proinflammatory fading media can be improved.

The invention discloses application of an LOX5 gene in tobacco variety improvement, and belongs to the technical field of plant genetic engineering. According to the invention, a tobacco lipoxygenase 5 coding gene is obtained from tobacco by using a homologous cloning technology, and an overexpression vector of the gene is constructed and transformed into a tobacco plant to obtain the tobacco plant overexpressed by the LOX5 gene. Through detection of LOX5 gene overexpression tobacco plants, it is found that the expression quantities of HPL, ADH and AAT genes of transgenic plants are significantly higher than those of control plants, the cis-3-hexenal content is increased by 36.19% compared with the control plants, and the cis-3-hexenol content is increased by 31.95% compared with the control plants. The result fully shows that the tobacco LOX5 gene has a great application prospect in the aspect of cultivating tobacco with high cis-3-hexenal and cis-3-hexenol contents.

The application discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. The lipoxygenase mutant disclosed by the application has the following mutation: S391G or S391A on the basis of the amino acid sequence shown in SEQ ID NO. 1. The lipoxygenase mutant provided by the application has obviously improved enzyme activity in catalyzing reaction, and improves the feasibility of industrial application.

The invention discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. According to the lipoxygenase mutant disclosed by the invention, on the basis of an amino acid sequence as shown in SEQ ID NO.1, the following mutations exist: H58E / I149K / A323G / R356S / S391G, D82I, D82V, S136V, S136A, I149R, I149K, A323G, R356S, A369G, N372R, V380W, S391G, S391A, S445G, S445A, D82I / I149K, D82I / S136V, H58E or H58D. The enzymatic activity and stability of catalytic reaction of the lipoxygenase mutant provided by the invention are obviously improved, and the feasibility of industrial application is improved.

The invention provides a strain of Yarrowia guilliermondii and application thereof. The preservation number of the Meyerozyma guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No. 34234, and the preservation number of the Meyerozyma guilliermondii is CGMCC No. 34234. The strain can secrete protease and lipoxygenase, and compared with a control group fermented by sterile water, the Yarrowia guilliermondii fermentation group can significantly increase the content of carotenoid degradation products, Maillard reaction products, chlorophyll degradation products, terpenoids and the like of tobacco leaves by applying the Yarrowia guilliermondii to enhanced fermentation of the tobacco leaves. Meanwhile, the aroma amount, aroma richness, maturity, sweetness and combustibility of the cigar tobacco leaves are remarkably improved, irritation and offensive odor are reduced, and the aroma characteristics of resin aroma, costustoot, nut aroma and the like are highlighted.

The invention belongs to the technical field of biological medicines, and particularly discloses an application of cumin in the aspect of neuroinflammation resistance, which is specifically as follows: lipopolysaccharide with the final concentration of 1 mu g / mL is used for inducing BV2 cells to form an acute neuroinflammation model, and cumin with gradient concentration is respectively added for treatment. Results show that 50-200 [mu] M cumin can significantly down-regulate expression of inflammatory factor nitric oxide, tumor necrosis factor-alpha and interleukin-6 secreted by lipopolysaccharide-induced BV2 cells. An immunoblotting method is used for discovering that cumin aldehyde can reduce expression of neuroinflammation 15-lipoxygenase / inflammasome signal channel targets in a dose-dependent manner, and meanwhile inflammatory reaction can be relieved by activating an oxidative stress signal channel. The results show that cumin has an anti-neuroinflammation effect. The cumin has a good application prospect for developing drugs or anti-inflammatory health-care products, cosmetics and food additives for treating inflammatory diseases by using cumin.

The invention provides a preparation process of yellow chickpea sprout peptide, the sprout peptide and application. The germinated peptide prepared by the invention is obtained by germinating yellow chickpeas to 0.8-1.2 cm and then hydrolyzing the germinated yellow chickpeas with a composite biological enzyme. The yellow chickpeas have the characteristics that 1, pretreatment is adopted before germination so as to inhibit the activity of lipoxygenase; 2, in the germination stage, the germination temperature of gradient heating is adopted; 3, a crushing mode of colloid mill treatment is adopted; and 4, carrying out ultrafiltration to intercept 1kDa, wherein the pH value of the final product is 5.5-6.0. The pretreated yellow chickpeas have the most abundant nutritional ingredients and the most vigorous life vitality in the bean germination stage, in the germination stage, the protein of the yellow chickpeas is activated by bioactive enzymes, various biological functions of life bodies are shown, and the germinated beans in the stage are the best choice as a protein raw material for preparing the bioactive peptide. Experiments prove that the composition has the efficacy of assisting in protecting gastrointestinal mucosal injury.

The application discloses a lipoxygenase mutant and application thereof, and belongs to the technical field of molecular biology and enzyme engineering. The lipoxygenase mutant disclosed by the application has the following mutations on the basis of the amino acid sequence shown in SEQ ID NO. 1: I149R, I149K or D82I / I149K. The lipoxygenase mutant provided by the application has significantly improved enzyme activity and stability in catalyzing reactions, and improves the feasibility of industrial application.

Various embodiments of the present disclosure relate to compounds having Formula I, Formula II, Formula IIA, Formula III, Formula IIIA, Formula IIIB, and / or pharmaceutically acceptable salts thereof. The compounds may be useful in the inhibition of lipoxygenase and / or the treatment of related diseases. In some embodiments, the subject compounds are useful in the preparation of compositions effective in the treatment of neurodegenerative diseases.

Disclosed in the present invention is a synergistic phytomedical mind and body wellbeing formulation involving a botanical composition with a dual approach to inhibit Cyclooxygenase (COX) and Lipoxygenase (LOX) enzymes that enhances nervine function for therapeutic intervention encompassing phytoceutical compositions and methods for treating inflammatory conditions, neurological and gut microbiome disorders. The formulation integrates a selected blend of natural ingredients with main active ingredients, including Stabilized Flax Meal, Cynatine® FLX, Sunflower Lecithin, Boswellia Extract, Turmeric / Curcumin C-3 Reduct®, Egg Shell Membrane, Green Oat Extract-Neuravena®, Hyaluronic Acid, Manganese Chelate and AstaReal® Astaxanthin are carefully chosen for their documented efficacy in addressing various dimensions of chronic pain and inflammation, as well as promoting joint / tissue health, gut health and cognitive function. Through synergistic action, these ingredients work in tandem to provide holistic relief from chronic inflammation, thereby improving quality of life without reliance on harsh pharmaceutical interventions.

Various embodiments of the present disclosure relate to compounds of Formula I, Formula II, Formula IIA, Formula III, Formula IIIA, Formula IIIB, and / or pharmaceutically acceptable salts thereof. The compounds may be useful for inhibiting lipoxygenase and / or treating related diseases. In some embodiments, the subject compounds are used to prepare compositions effective for treating neurodegenerative diseases.

The present invention belongs to the field of biomedicine and specifically relates to a purine derivative, its synthesis method, and application. The structure of the purine derivative is shown in Formula I, wherein R1 is a saturated hydrocarbon group, alkenyl group, alkynyl group, cycloalkyl group, aryl group, heterocyclic group, or alkoxycarbonyl group; R2 is hydrogen, halogen, alkyl group, aryl group, amino group, hydrocarbon amino group, or alkoxy group; R3 is hydrogen, alkyl group, amino group, hydrocarbon amino group, alkoxy group, aminosulfonyl group, sulfanyl group, aryl group, heterocyclic group, or carboxyl group; and n is 1, 2, 3, 4, 5, or 6. The purine derivative has excellent inhibitory activity against 5-lipoxygenase and 5-lipoxygenase, can be used to prepare lipoxygenase inhibitors, and can also be used to prepare drugs for treating lipoxygenase-mediated diseases, and has good clinical application prospects.

Molecules for inhibiting arachidonate 15-lipoxygenase (ALOX-15) gene products including dsRNA (dsRNA) agents such as small interfering RNAs (siRNAs) for therapeutic use, additionally, methods to inhibit the expression of a target gene by administering these agents for the treatment of diseases involving ALOX-15 gene products.

The present invention provides a passion fruit PeLOX2 gene, the nucleotide sequence of which is shown in SEQ ID NO: 1. The present invention provides a passion fruit PeLOX2 gene cloned from passion fruit for the first time. The gene can respond to fruit ripening, reduce the size of the fruit, accelerate fruit ripening, improve drought resistance, and make the leaves of the plant slender. The present invention provides a new candidate gene for research on improving plant stress resistance, accelerating plant fruit ripening, and changing plant leaf and fruit size.

The invention discloses mung bean plant milk with low beany flavor, low bitter taste and high stability and a preparation method of the mung bean plant milk, belongs to the technical field of processing of vegetable protein beverages, and aims to solve the technical problems that existing mung bean beverages are obvious in beany flavor, bitter and astringent in taste, easy to precipitate or flocculate during storage and the like. The specific preparation method comprises the following steps: firstly, carrying out heat treatment on a mung bean raw material to passivate lipoxygenase; pulping the treated raw materials, and sequentially carrying out step-by-step enzymolysis by adopting neutral protease, alpha-amylase, saccharifying enzyme and cellulase; and finally, carrying out high-pressure homogenization on the enzymatic hydrolysate. The prepared mung bean plant milk has the characteristics of low beany flavor, low bitter taste, high stability and the like, is light in flavor and smooth in mouth feel, and can realize long-term stability of a system under the condition of not additionally adding an emulsifying agent and a thickening agent.

The present invention discloses the use of a lipoxygenase gene LOX6 in regulating the development of rice spikelets. The present invention obtains a rice spikelet development-abnormal mutant through EMS mutagenesis, and isolates the spikelet development-control gene LOX6 through the MutMap+ method. This gene encodes a lipoxygenase. Mutation of this gene causes the 917th amino acid of LOX6 to change from proline to leucine, resulting in abnormal development of rice spikelets. The present invention further obtains a LOX6 mutant through CRISPR / Cas9 knockout, and finds that the spikelets of the knockout plants develop abnormally, that is, mutations in the lipoxygenase gene LOX6 affect the development of rice spikelets. The research on the lipoxygenase gene LOX6 in the present invention provides a theoretical basis for the cultivation of transgenic rice such as multi-flowered spikelets.

The invention discloses an anti-inflammatory soothing scalp care composition for improving a scalp microenvironment. The anti-inflammatory soothing scalp care composition comprises any one or more of rhamnetin, diosmetin, quercitrin and kaempferol as functional active ingredients. The anti-inflammatory soothing scalp care composition provided by the invention can regulate the levels of arachidonic acid lipoxygenase-5, arachidonic acid lipoxygenase-12 and arachidonic acid lipoxygenase-15, can reduce the levels of inflammatory factors IL-6, IL-8 and TNF-alpha, and has the effects of regulating the scalp microenvironment, relieving scalp inflammation and soothing scalp.

The application discloses a preparation method of whole wheat flour with controlled lipase and lipoxygenase activity, and relates to the technical field of whole wheat flour processing and storage. The method comprises the following steps: adopting DBD plasma to treat the bran of wheat grains, and then directly grinding the wheat grains to obtain the whole wheat flour. The method controls the lipase and lipoxygenase in the bran of the wheat grains, thereby stabilizing the whole wheat flour, prolonging the storage period of the whole wheat flour, and improving the nutritional quality and processing quality of the whole wheat flour. The DBD plasma treatment is performed at a power of 300-500 W for 60-180 s.