91 results about "Lipoxygenase activity" patented technology

A stabilized bran component and a stabilized whole grain wheat flour containing the component are produced by subjecting a bran-enriched coarse fraction which contains bran, germ and starch to grinding or milling to reduce grittiness of the coarse fraction without substantially damaging the starch due to abrasion. The coarse fraction may be stabilized by heating to substantially reduce the lipase and lipoxygenase activity of the coarse fraction without substantial gelatinization of the starch. High levels of natural antioxidants and vitamins are retained while avoiding substantial acrylamide formation during the stabilization. The stabilized coarse fraction or stabilized bran component may be combined with a fine fraction which contains predominantly endosperm to obtain a stabilized whole grain wheat flour. The stabilized whole grain wheat flours and the stabilized bran component exhibit extended shelf life and may be used for making baked goods, such as cookies, with desirable oven spread, and a non-gritty mouthfeel.

There is provided a compound of formula (I), wherein R¹, R², R³, X and Y have meanings given in the description, and pharmaceutically-acceptable salts thereof, which compounds are useful in the treatment of diseases in which inhibition of the activity of a lipoxygenase (e.g. 15-lipoxygenase) is desired and/or required, and particularly in the treatment of inflammation.

The invention provides a novel herbal composition containing the extracts of the leaves and/or stem of <italic>Argemone mexicana </highlight>plant, optionally containing the extracts of the fruits of <italic>Cuminum cyminum</highlight>, which exhibits useful in vitro, in vivo and interesting immunological and pharmacological activities; a process for preparation thereof; and a method of treatment of psoriasis and related immunological and biological disorders by administration of the said novel herbal composition. The useful in vitro, in vivo and interesting immunological and pharmacological activities exhibited by the extracts and fractions of the leaves and/or stem of <italic>Argemone mexicana </highlight>plant include immunosuppression, lymphoproliferation inhibition, cytokine modulation such as IL-2 inhibition, IFNgamma inhibition, IL-10 induction, keratinocyte proliferation inhibition, keratolytic activity, endothelial cell proliferation inhibition, inhibition of cell adhesion molecule expression such as ICAM-1, MEST inhibition, and enzymes inhibition such as p60src Tyrosine kinase, which are known to be involved in anti-psoriatic activity. The novel herbal composition(s) is useful in the treatment of various disorders, such as psoriasis including plaque psoriasis, gutatte psoriasis, pustular psoriasis and psoriasis of the nails; dermatitis and scleroderma; eczema; inflammatory disorders and other autoimmune diseases like psoriatic arthritis, rheumatoid arthritis, Crohn's disease, multiple sclerosis, irritable bowel disease, ankylosing spondilitis, systemic lupus erythremetosus and Sjogren's syndrome; allergies like asthma and chronic obstructive pulmonary disease and is safe, well-tolerated, non-toxic, with minimal and reversible adverse reactions or side effects, and most importantly, with minimal relapse or recurrence of the disease following completion of a treatment regimen. The invention also describes the presence of phosphodiesterase (III, IV and V) inhibition and 5-Lipoxygenase inhibition in the aqueous, ethanolic or aqueous-ethanolic extracts of fruits of <italic>Cuminum cyminum </highlight>plant.

This invention relates to an herbal composition for the treatment and remedy of bronchial respiratory difficulties, more particularly this invention describes the process of separation, physicochemical characterization and biological response evaluation of active components obtained from extracts of any plant parts including leaves, barks, roots and seeds of plants M. koenigii and P. betle in order to establish their role on the treatment and remedy of bronchial respiratory troubles.

There is provided compounds of formula I,

wherein R¹, R², X¹, X² and n have meanings given in the description, and pharmaceutically-acceptable salts thereof, which compounds are useful in the treatment of diseases in which inhibition of the activity of a lipoxygenase (e.g. 15-lipoxygenase) is desired and/or required, and particularly in the treatment of inflammation.

The present invention provides oxazolyl and thiazolyl inhibitors of 15-LO, pharmaceutical compositions containing such inhibitors and methods for treating diseases related to the 15-LO cascade using such compounds and compositions.

Disclosed are compositions which include an isolated soft tissue from Modiolus modiolus (“MM”) and compositions which include an oil isolated from MM or a portion thereof. Also disclosed are methods of treating adenocarcinoma in a subject; methods of decreasing proliferation of adenocarcinoma cells, or of inducing apoptosis of adenocarcinoma cells, or of inducing differentiation of adenocarcinoma cells into non-cancerous cells; methods of inhibiting the activity of 5-lipoxygenase or 12-lipoxygenase or both in cells; and methods of treating a subject suffering from a disease or condition associated with excessive 5-lipoxygenase activity or 12-lipoxygenase activity or both. A process for preparing an MM extract by contacting MM or a portion thereof with a solvent under conditions effective to extract one or more materials from the MM or portion thereof is also described.

The invention relates to a recombined lipoxygenase PhLOX and a preparation method thereof. The PhLOX has activities of a lipoxygenase, a hydroperoxide lyase and a propadiene oxide synthetase at the same time. The method includes following steps: extracting total RNA from porphyra haitanensis; designing a primer according to a gene sequence of the lipoxygenase; amplifying an overall-length cDNA sequence through an RACE technology; recombining the overall-length cDNA sequence to an expression vector pET-28a; converting escherichia coli E.coli BL21; screening out positive clone; and generating the multifunctional lipoxygenase of a bacteria liquid with an expression quantity being 2mg/L through inducible expression. Compared with a lipoxygenase in the prior art, the recombined lipoxygenase has a plurality of enzyme functions, is free of a strict substrate specifity and allows downstream products in various structures to be formed.

The present invention relates to an extract isolated from Sophorae Radix useful for prevention and treatment of respiratory diseases. More specifically, the present invention relates to a pharmaceutical drug comprising the Sophorae Radix extract having excellent effects of inhibiting airway contraction, respiratory infections, 5-lipoxygenase activity, phosphodiesterase 4 activity, airway hyper-responsiveness and airway remodeling; antagonistic activity against leukotriene D4; and an antitussive effect, thus being useful for prevention and treatment of respiratory diseases such as asthma, acute or chronic bronchitis, allergic rhinitis, acute upper respiratory infections and acute lower respiratory infections, and the like.

The invention discloses a method for testing the activity of corn seed embryo lipoxidase. The method comprises the following steps of: reacting an enzyme activity detection system at temperature of 24 to 26 DEG C, testing outside dimension (OD) values of the system at a position of 234 nanometers after reaction for t1 and t2, and recording the values as OD(t1) and OD(t2); and calculating the activity of the lipoxidase of the corn seed embryo according to the following formula: A=60s*[OD(t2)-OD(t1)]/0.01/(t2-t1). The enzyme activity detection system consists of tween 20, linoleic acid, Tris-Hc1 buffer solution with pH value of 8.0 and concentration of 0.02 mol/ml and the corn seed embryo lipoxidase to be tested, wherein the concentration of the linoleic acid is 0.3mmol/L, the concentrationof tween 20 is 0.25mmol/L; and the concentration of the corn seed embryo lipoxidase to be tested is not more than 0.0167mg/3ml. The enzyme activity is defined that: the enzyme amount required by the enzyme activity detection system for increasing 0.01 absorbance at every minute is an enzyme activity unit.

The invention belongs to the field of crop fertilizers, and particularly relates to a special fertilizer for enhancing the original aroma of apples. The special fertilizer contains ammonium nitrate, monopotassium phosphate, potassium sulfate, calcium humate, magnesium sulfate, sodium borate, fulvic acid iron, an amino acid and an aroma enhancing activating agent. The special fertilizer is characterized in that the nutrient contents of nitrogen, phosphorus and potassium are reasonably compounded according to the nutrition uptake characteristics of the apples during growth, medium trace elements: calcium, magnesium, boron and iron, which are needed during the young fruit period and the fruit swelling period of the apples are added in the special fertilizer, so that the growth and the development of the apples are promoted, and the yield of the apples is increased; besides, the amino acid and the aroma enhancing activating agent are added in the fertilizer, the amino acid is a substrate which is synthesized from the volatile aromatic esters of the apples, the aroma enhancing activating agent can promote the activity of transaminase and lipoxygenase in the apples and increase the contents of the aromatic esters and alcohols, so that the original aroma of the apples is stronger. When the special fertilizer is applied in the young fruit period and the fruit swelling period of the apples, not only the yield of the apples can be increased, but also the original aroma of the apples can be enhanced. The special fertilizer has a relatively high market application value.

There is provided compounds of formula (I) wherein W is an optionally substituted aryl or heteroaryl group, and pharmaceutically-acceptable salts thereof, which compounds are useful in the treatment of diseases in which inhibition of the activity of a lipoxygenase (e.g. 15-lipoxygenase) is desired and/or required, and particularly in the treatment of inflammation.

The invention discloses a method for suppressing soybean lipoxygenase activity by use of cyclodextrin, belonging to the technical field of cyclodextrin. According to the invention, the cyclodextrin is subjected to complexation with hydrophobic amino acid of the soybean lipoxygenase molecules so as to change the active conformation of lipoxygenase and suppress the activity of the lipoxygenase by 86.26% maximally. The method disclosed by the invention is simple and easy to implement, saves energy, has low cost, realizes good working environment and sanitary environment, and avoids pollution; and through the method, the deodorization technology for soybean and products thereof can be simplified.

The present invention provides a novel composition of matter comprised of a mixture of two specific classes of compounds --Free-B-Ring flavonoids and flavans-- for use in the prevention and treatment of diseases and conditions associated with mouth, gums and teeth. This composition of matter simultaneously inhibits cyclooxygenase (COX) and lipoxygenase (LOX) enzymatic activity and reduces cytokine production at the mRNA level in normal, aged and damaged periodontal cells and tissues. This invention further provides a method for the prevention and treatment of diseases and conditions of the mouth, gums and teeth. The method for preventing and treating diseases and conditions of the mouth, teeth and gums is comprised of administering to a host in need thereof a therapeutically effective amount of a composition comprising a mixture of Free-B-Ring flavonoids and flavans synthesized and/or isolated from a single plant or multiple plants, preferably in the Scutellaria, Oroxylum, Acacia or Uncaria genus of plants and pharmaceutically and/or cosmetically acceptable carriers. Finally the present invention provides a method for the prevention and treatment of diseases and conditions of the mouth, teeth or gums, including but not limited to periodontal diseases, such as gingivitis, periodontitis, pulpitis, periodontal conditions caused by the physical implantation of oral dentures, trauma, injuries, bruxism, neoplastic and other degenerative processes; material alba, pellicles, dental plagues, calculus, and stains. Use of the composition described herein also affords the benefit of maintaining optimum saliva production and pH, minimizing bacterial growth, reducing the formation of pellicles and plague, inhibiting tooth decalcification and tooth caries (decay), promoting remineralization, which yields healthy gums, whitening teeth, maintaining healthy oral hygiene and reducing oral malodour (halitosis).

Methods of use and compositions useful for reducing lines and wrinkles, evening skin tone, lightening skin tone, increasing skin radiance, elasticity, skin barrier function, and/or skin firmness, reducing photodamage, reducing sagging skin, inhibiting anti-oxidant capacity in skin, MMP-1, MMP-3, MMP-9, pro-inflammatory cytokines (e.g., lipoxygenase, IL-6, Il-8, TNF-α, or VEGF), and/or elastase expression in skin, and/or increasing collagen expression in skin, elastin expression in skin, laminin expression in skin, and/or fibronectin expression in skin are disclosed herein. The compositions can additionally reduce or mitigate unwanted side effects shown in other retinol products such as erythema or redness in skin, skin dryness, peeling or flaking of skin, and/or skin irritation including without limitation, burning, stinging, itching, or tingling. The composition includes a combination of one or more of Rosmarinus officianalis leaf extract, Morus alba fruit extract, tetrahexyldecyl ascorbate, retinol, Acmella oleracea extract, Alpinia galanga leaf extract, Argania spinosa oil, and/or saccharide isomerate.

The invention belongs to the field of medicines, and particularly relates to a composition for preventing or treating osteoporosis, a preparation of the composition and application of the composition in preventing or treating osteoporosis. Specifically, the composition disclosed by the invention is mainly composed of a GPX4 agonist and a lipoxygenase 15 (Alox-15) inhibitor, and vitamin E is supplemented to play a synergistic effect. In one embodiment, the GPX4 agonist is prepared from one or more of 6-gingerol, 8-gingerol, 10-gingerol and PKUMDL-LC-101-D04, and the GPX4 agonist is prepared from one or more of 6-gingerol, 8-gingerol, 10-gingerol and PKUMDL-LC-101- And the Alox-15 inhibitor is prepared from one or more of quercetin, ferulic acid, baicalein, hesperetin, nobiletin, PD146176, orange flavone and ML351. In another embodiment, the composition comprises a ginger extract, a sea buckthorn extract, and a wheat germ extract. Pharmacological experiment results show that the composition can effectively prevent, improve and treat osteoporosis and clinical related symptoms.