71 results about "Lipid oxidation" patented technology

The invention discloses a preparation method of liposome for encapsulating euphausia superba oil, and belongs to the field of euphausia superba oil. Through material selection and hierarchical structure design, starch octenyl succinate, sodium alginate and pea protein are screened as composite wall materials, and the materials can achieve a synergistic effect on the molecule-interface-micro-nano scale to delay lipid oxidation, control the targeted release characteristic of active ingredients and improve the absorption efficiency of fat-soluble ingredients. Moreover, the liposome raw material of the euphausia superba oil is screened, and the liposome with low particle size and high stability is prepared.

The invention discloses a composition formula for assisting in protecting the liver and dispelling the effects of alcohol, and relates to the field of health food, the composition has an assisting effect on protecting the liver and dispelling the effects of alcohol, and the composition comprises the following composition components: milk thistle (0.466-0.482), puerarin (0.069-0.075), globe artichoke extract (0.065-0.072), glutathione (0.04-0.049), dandelion extract (0.151-0.164) and hydrogen butyric acid (0.146-0.162). By adopting the composition formula for assisting in protecting the liver and eliminating the alcohol, the composition has obvious effects of inhibiting the liver from generating lipid oxide and assisting the human liver in eliminating oxide substances, and meanwhile, the composition can be used for preventing lipid peroxidation damage by improving the activity of glutathione reductase and improving the antioxidant effect; therefore, the effects of protecting the liver and reducing injury are achieved. The components of the composition are equal-part particles formed by physical stirring and uniform mixing, the components are complementary and synergistic, the composition is safe and free of side effects to human bodies, the raw materials of the composition formula are convenient to purchase, the production benefits are high, and wide application is facilitated.

The invention discloses an application of platycladus orientalis essential oil in the field of anti-oxidation and immunity-enhancing medicines / foods, and belongs to the technical field of food health care, the main component of the adopted platycladus orientalis essential oil is cedrol, the relative content is 33.7%, and other components comprise esters, cuparene, thujopsene and the like. The administration dosage is determined through an acute toxicity test, and the platycladus orientalis essential oil is determined to have the functions of resisting oxidation and enhancing immunity by measuring the content of SOD, GSH, protein carbonyl and lipid oxidation product MDA in animal tissues and through an immune experiment.

The invention provides a water-soluble rosemary antioxidant. The antioxidant is prepared by mixing a rosemary extract, edible oil, a protein aqueous solution and a polysaccharide aqueous solution, wherein the mass volume concentration of the protein aqueous solution and the polysaccharide aqueous solution is 3%-4%, and the mass ratio of the protein aqueous solution to the polysaccharide aqueous solution is 1: (1-3); the ratio of the total mass of the rosemary extract and the edible oil to the total mass of the protein aqueous solution and the polysaccharide aqueous solution is 1: (1-4). The raw materials of the antioxidant prepared by the invention are all natural harmless raw materials, do not contain chemical reagents, and have a cleaning label; the water solubility and the stability are good, and meanwhile, the stability can still be kept under different pH conditions; the food preservative can obviously delay the occurrence time of lipid oxidation in food, and is suitable for preservation of various foods.

The invention provides a method for improving the oxidation resistance of phenylalanine based on N-nicotinoyl modification and application of the method. According to the method, N-nicotinoyl phenylalanine amide with antioxidant activity is obtained through chemical synthesis or enzymatic synthesis, and the antioxidant performance of N-nicotinoyl phenylalanine amide is evaluated by using an ABTS free radical scavenging method. Results show that the N-nicotinoyl phenylalanine amide can delay lipid oxidation in a food system, prolong the shelf life of the product and maintain the nutritional stability; in a cosmetic system, skin oxidative damage can be relieved, skin aging can be delayed, and skin color can be improved. The compound disclosed by the invention can show a remarkable antioxidant effect under the condition of a relatively low addition amount, so that a safe and effective antioxidant is provided for the fields of foods and cosmetics, and the compound has a good application value and a good popularization prospect.

The invention provides an application of phosphatidylcholine 36: 1 in prevention and treatment of senescence cognitive impairment, and particularly relates to an application of phosphatidylcholine 36: 1 or a pharmaceutical composition containing phosphatidylcholine 36: 1 in preparation of drugs for prevention and / or treatment of senescence cognitive impairment. The phosphatidylcholine 36: 1 can improve the level of superoxide dismutase and glutathione peroxidase in the brain of the mouse and reduce the lipid oxidation level at the same time. After the phosphatidylcholine 36: 1 is used for treatment, the levels of inflammatory factors interleukin 1 beta, interleukin 6 and tumor necrosis factor alpha in the brain are obviously reduced. Meanwhile, neurotransmitter disorder in brains of senescent mice can be remarkably improved. After qRT-PCR analysis is carried out in the brain of a mouse, it is found that after phosphatidylcholine 36: 1 treatment, the senescence gene level is remarkably reduced, the pro-apoptosis gene level is remarkably reduced, and the anti-apoptosis gene level is remarkably increased. And after in vitro administration of phosphatidylcholine 36: 1 to mouse hippocampal neuronal cells, no cytotoxicity is found. Therefore, the phosphatidylcholine 36: 1 can be used for preventing and treating senescence cognitive impairment.

The invention discloses a lipid oxidative metabolism characteristic analysis method and system based on REIMS and LC-MS / MS, and belongs to the technical field of lipid oxidative metabolism detection. In order to solve the detection problems of various oxidized lipids, extremely low content in a biological sample and large metabolic fluctuation, the method provided by the invention optimizes a biological sample pretreatment process, sets accurate REIMS and LC-MS / MS technical parameters, adopts two mass spectrometry technologies for combined detection and adopts an internal standard method for quantification, so that the content of the oxidized lipids in the biological sample is greatly reduced, and the content of the oxidized lipids in the biological sample is greatly reduced. And accurate qualitation and quantification of trace lipid oxidation products in a biological sample are realized. The method has the advantages that the trace lipid oxidation products in the biological sample can be accurately qualitative and quantitative, and reliable data support is provided for mining novel biomarkers.

The invention discloses glycyrrhiza polysaccharide for preventing and treating acute stomach injury and a preparation method and application thereof. The molecular weight of the glycyrrhiza polysaccharide is 60 kDa, the percentage content of protein is smaller than or equal to 5%, and the mass percentage content is larger than or equal to 60% according to glucose; the preparation method comprises the following steps: carrying out ethanol reflux degreasing on liquorice, carrying out water extraction and alcohol precipitation, removing protein by adopting Sevag, dialyzing by using a 8-13 kDa dialysis bag, carrying out rotary concentration, and freeze-drying to obtain the glycyrrhiza polysaccharide. Animal experiments show that the glycyrrhiza polysaccharide has a protective effect on acute alcoholic stomach injury, the content of MDA and MPO in animal stomach tissues can be reduced, and the stomach injury is relieved by reducing lipid oxidation and neutrophil infiltration level; the content of gastric tissue pepsase and VEGF (vascular endothelial growth factor) can be increased, and the gastric mucosa can be protected in the aspects of maintaining the stomach environment, promoting blood vessel healing and reducing the injury time and degree; and the medicine also has a pathological repair effect on acute stomach injury.

The invention relates to an omega-3 lipid detection method based on an inorganic matrix enhanced MALDI mass spectrum. The invention discloses an omega-3 supplement identification technology of an inorganic matrix enhanced MALDI mass spectrum, which can efficiently and accurately carry out comprehensive analysis on lipid types, double bond positions and oxidation degrees in a complex lipid sample. The technology comprises the following steps: selecting an omega-3 supplement, mixing the omega-3 supplement, a cycloaddition reagent and an inorganic matrix, carrying out MALDI laser-driven online cycloaddition reaction, and analyzing the lipid double-bond isomer by mass spectrum data. The selected 25nm hydrophilic anatase titanium dioxide obviously improves the sensitivity of lipid double bond identification, the vacuum environment of the MALDI mass spectrum and shorter laser irradiation time (30s) effectively protect lipid from additional oxidation, and the original oxidation information of the lipid is retained. The method successfully identifies 30 main omega-3 lipids in four omega-3 supplements, and reveals significant differences of lipid types, omega-3 purity and lipid oxidation degrees. The method has a wide application prospect in the aspect of high-efficiency and high-throughput screening of other complex lipid information samples such as omega-3 supplements and the like.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The invention discloses a model compound-based lipid oxidation free radical specificity induction and detection method, which comprises the following steps: catalyzing a model compound (cumene / cumene hydroperoxide) or a lipid sample by using iron ions (Fe < 2 + > / Fe < 3 + >) with different valence states, and specifically inducing to generate cumene alkyl free radicals, cumene peroxide free radicals and cumene alkoxy free radicals; the method comprises the following steps: reacting a free radical trapping agent PBN with active free radicals to generate a stable PBN-free radical adduct; the adduct is separated and qualitatively identified by utilizing HPLC-TOF / MS (High Performance Liquid Chromatography-Time of Flight / Mass Spectrometry), and is accurately quantified by HPLC-UV (High The method has the advantages that three key free radicals can be specifically distinguished and quantified, the sensitivity is high, the repeatability is good, the method is suitable for free radical analysis from a model system to a complex grease sample, and a reliable analysis means is provided for research on a lipid oxidation mechanism.

The use of a cell-permeant molecular probe to assay the presence, and monitor the production, of the reactive oxygen species, superoxide, in red blood cells (RBCs) is described. The assay is performed using electron paramagnetic resonance (EPR) spectroscopy and a cell-permeant hydroxylamine spin trap. The cell-permeant molecular probe is not detected or quantifiable by EPR in its reduced form. However, upon entering the RBCs, the probe is oxidized by reacting with superoxide to yield a stable nitroxide radical that is detected and quantified using EPR. Our studies indicate that superoxide production is consistent with RBC metabolism that predicts lipid oxidation, membrane injury and poor post-transfusion performance. Therefore, measurement of reactive oxygen species can be used as an indicator of RBC quality at the time of donation and after storage.

、To provide a method for determining the smell of fish, especially the degree of smell deterioration and lipid oxidation of tilapia during the refrigeration period. 【Solution means】Steps of obtaining raw materials by killing fresh tilapia, taking slices, washing, and mixing and grinding the fish meat; steps of extracting lipids from the raw materials; steps of dividing the obtained lipids, storing them under low-temperature conditions, and measuring the degree of lipid oxidation and lipid metabolism data of tilapia at different refrigeration times by lipid metabolomics technology after the storage ends; steps of constructing a lipid metabolism network related to smell and analyzing the degree of lipid oxidation and metabolism data at different refrigeration times based on the lipid metabolism network to obtain metabolic markers corresponding to a plurality of lipids; steps of screening the obtained metabolic markers to obtain evaluation metabolic markers; steps of analyzing the degree of lipid oxidation and metabolic status of a plurality of lipids based on the evaluation metabolic markers corresponding to the plurality of lipids.

The present invention discloses the use of the East Asian scorpion toxin polypeptide Makatoxin‑3 or a pharmaceutical composition containing the same in the preparation of a drug for treating and / or preventing liver diseases caused by lipid accumulation in the liver, in particular, in the preparation of a drug for treating and / or preventing non-alcoholic fatty liver disease. The present invention finds that Makatoxin‑3, as a PPARα agonist, significantly activates the transcription of genes related to fatty acid β-oxidation, activates the transcription of genes related to fatty acid β-oxidation, promotes lipid oxidation and decomposition to inhibit lipid accumulation in the liver, and reduces inflammation and liver fibrosis. Therefore, Makatoxin‑3 can be used to prepare a drug for treating non-alcoholic fatty liver disease.

The application provides a method for monitoring lipid oxidation tendency, and belongs to the technical field of fluorescent probe detection. The method utilizes the fluorescence characteristics of a BODIPY molecular probe, and through selecting a molecular probe with a specific structure, directional reaction occurs between the BODIPY molecular probe and oxygen free radicals generated by lipid oxidation at a specific pH value. The conjugated system of the BODIPY molecular probe is destroyed, the half-chlorin structure is broken, and the fluorescence color changes from red to blue-green. According to the change of the fluorescence color before and after the reaction, the visualization monitoring of the lipid oxidation tendency and spatial distribution is realized through fluorescence imaging.

To provide a method for determining odor of fish, especially odor deterioration and lipid oxidation degree of tilapia during cold storage.SOLUTION: The method includes the steps of: killing fresh tilapia, slicing, washing, mixing and grinding the fish meat to obtain raw materials; extracting lipids from the raw materials; dividing the obtained lipids and storing them under low-temperature conditions, and after storage, measuring the lipid oxidation degree and lipid metabolic data of tilapia at different refrigeration times using lipid metabolomics technology; constructing an odor-related lipid metabolic network and analyzing the lipid oxidation degree and metabolic data at different refrigeration times based on the lipid metabolic network to obtain metabolic markers corresponding to multiple lipids; screening the obtained metabolic markers to obtain evaluation metabolic markers; and analyzing the oxidation degree and metabolic status of multiple lipids based on the evaluation metabolic markers corresponding to multiple lipids.SELECTED DRAWING: Figure 1

The invention belongs to the field of food processing, and particularly relates to a processing method of tea-flavored fresh braised chicken capable of prolonging the shelf life and an obtained product. According to the processing method provided by the invention, under the action of the tea polyphenol, on one hand, free radicals in a lipid oxidation chain reaction are competitively removed, grease rancidity and protein oxidative degradation are inhibited, and the rising rate of putrefaction indexes such as volatile basic nitrogen and a peroxide value is reduced; on the other hand, the cell membrane integrity of escherichia coli, staphylococcus aureus and other pathogenic bacteria and molds is destroyed, and metabolism and reproduction are inhibited. After the process is optimized, the shelf life of the Texas braised chicken under the normal-temperature vacuum packaging condition is prolonged to 15-20 days compared with that of the Texas braised chicken in the traditional process, artificially synthesized preservatives do not need to be added in the whole process, and the Texas braised chicken conforms to the natural and healthy food consumption trend.

The invention provides a method for inactivating buckwheat lipoxygenase activity by using superheated steam treatment, which comprises the following steps: S1, shelling treatment: milling and shelling buckwheat seeds to obtain buckwheat core powder; s2, superheated steam treatment: enabling the buckwheat core flour to be in full contact with superheated steam at the temperature of 150-190 DEG C, wherein the treatment time is 3-15 seconds; and S3, collecting and storing: collecting the buckwheat core powder treated in the step S2, cooling to room temperature, and sealing and storing the buckwheat core powder in a self-sealing bag. According to the method, through short-time high-temperature superheated steam treatment, lipid oxidation is inhibited while key deterioration enzyme systems in buckwheat are efficiently inactivated, storage rancidity is remarkably delayed, and the method has the advantages of high efficiency, quality guarantee and industrial applicability.

This invention discloses a method for pretreating camellia seeds using low-temperature plasma, relating to the field of bioengineering technology. The method involves discharging the entire surface of the camellia seed using DBD, JET, DF, or RF at atmospheric pressure, preferably DBD 120kV×1min or RF 3m / min at a distance of 0-3mm for 20 passes. This achieves microporousization of the seed coat and targeted disruption of the cell wall to promote mass transfer. The treatment reduces lipase and lipoxygenase activities by approximately 31.72% and 35.69% (or 29.54%), respectively, and significantly enhances the antioxidant capacity of total phenols and DPPH / ABTS / FRAP in the methanol extract (up to 1.58 times the total phenols). This method simultaneously promotes the release of active ingredients, inhibits lipid oxidation, and improves oil yield and quality under low-temperature, non-thermal conditions, making it suitable for continuous industrial applications.

The invention belongs to the technical field of food processing, and particularly relates to a method for delaying lipid oxidation in the frozen storage process of conditioning meat products. According to the method, the fresh meat product is firstly subjected to vacuum cooking, and then the conditioned meat product subjected to vacuum cooking is placed in a ternary dipping freezing system to be frozen for 1-2 h at the temperature of-30 DEG C to-20 DEG C. The treatment method has the beneficial effects that after the fresh meat product is treated by adopting the method in combination with conditioning and rapid freezing, the lipid oxidation rate of the conditioned meat product is remarkably reduced in the subsequent freezing storage process, meanwhile, the second-stage and third-stage structural stability of protein is higher, and the treatment method provided by the invention has the advantages that the treatment effect is good, and the treatment cost is low. The raw materials are simple and easy to obtain, the treatment cost is low, and the universality is higher.

The invention relates to a ganoderma lucidum fermentation product and a preparation method and application thereof, and belongs to the field of medicine and health care products. The ganoderma lucidum fermentation product is prepared from total sugar, fat, total protein, polypeptide, vitamin A, vitamin E, vitamin C and total terpenoids according to the mass ratio of (2500 to 5000) to (100 to 500) to (1500 to 3000) to (100 to 500) to (1 to 50) to (10 to 50) to (20 to 100) to (200 to 500). The ganoderma lucidum fermentation product disclosed by the invention can improve lipid oxidation caused by radiation, so that the effects of maintaining body health and the like are achieved; the compound can be used for preparing medicines or health-care products for preventing and treating radiation.

A polyhydroxyalkanoate (PHA) containing monomer units of 3-hydroxybutyrate for use, by oral administration, in the prevention or treatment of an overweight or obesity condition, or of metabolic dysfunctions related to the condition. A marked effect was observed on the reduction of the body weight, both in the case of a standard diet and in the case of a diet rich in fats, a reduction that can be attributed to an increase in lipid oxidation to the detriment of the deposit of excess lipids, as confirmed by the decrease in the body weight and by analyses of the body composition. A positive effect on the metabolic dysfunctions related to an overweight or obesity condition has also been verified, in particular on dyslipidemias, such as the blood level of low-density lipoproteins (LDL cholesterol) and triglycerides, as well as an improvement in the inflammatory state.

The invention provides a plant antioxidant for conditioning chicken, and relates to the field of food additives, the plant antioxidant is composed of sophora flower, pomegranate peel, wild chrysanthemum flower, rosemary, cinnamon, mint, schizonepeta and sea-buckthorn; the plant antioxidant disclosed by the invention has relatively strong antioxidant capacity, especially shows a remarkable effect in the aspect of removing DPPH free radicals and ABTS < + > free radicals, in addition, the plant antioxidant not only can effectively inhibit and condition lipid oxidation in the chicken storage period and reduce the TBARS value, but also can maintain good color stability and inhibit microbial growth, and the plant antioxidant has the advantages of being safe and reliable. The sensory quality of the conditioned chicken is remarkably improved, and a safe, effective and natural solution is provided for storage and preservation of the conditioned chicken.

The invention discloses a tea polyphenol ester-phytosterol ester liposome and a preparation method of a layer-by-layer self-assembly coating, and the preparation method comprises the following steps: (1) mixing an active substance with phospholipid, glycerol, phytosterol ester and tea polyphenol palmitate in a buffer solution, and carrying out post-treatment to obtain liposome carrying the active component; (2) adding the liposome carrying the active component into the acidified gelatin solution, incubating, and centrifuging to remove free gelatin, so as to obtain gelatin-coated liposome; and dropwise adding the gelatin-coated liposome into a sodium hyaluronate solution, reacting, and centrifuging to obtain the sodium hyaluronate-gelatin coating modified layer-by-layer self-assembled liposome. The method solves the technical problems of organic solvent residue, limited phytosterol application and lipid oxidation caused by heating, greatly shortens the production time, and is suitable for large-scale industrial production. In addition, the liposome prepared by the method is proved to have good stability and antioxidant activity, and can be widely applied to the fields of foods, daily chemicals and medicines.

The application discloses a CAR-T cell regulated by an ACSL4 gene and a preparation method and application thereof. The application improves the tolerance of the CAR-T cell to in-vivo lipid oxidation stress by regulating the ACSL4 related lipid metabolism process of the CAR-T cell, thereby prolonging the in-vivo persistence and treatment effect of the CAR-T cell, and provides a new technical scheme for the preparation and optimization of a cell immunotherapy product.

The invention discloses a saussurea involucrata polysaccharide, a nanoemulsion thereof, and a preparation method and application of the saussurea involucrata polysaccharide. The specific method comprises the following steps: firstly, drying and grinding saussurea involucrata peel into powder; selecting bacillus altitudinis cultured to the second generation as a fermentation strain, carrying out inoculation fermentation treatment on the powder, and filtering to obtain supernate; and adding ethanol for alcohol precipitation overnight, centrifuging, performing rotary evaporation, removing protein by a Sevage method, dialyzing, purifying, freeze-drying, and repeating the operation to obtain the product. According to the present invention, the Prunus persica pericarp polysaccharide is extracted and purified, the standardized process is established, the Prunus persica polysaccharide nano-emulsion is prepared, and the high encapsulation efficiency and the stability are achieved by optimizing the surfactant system; according to the invention, the saussurea nipponica polysaccharide and the nanoemulsion thereof are innovatively applied to shrimp coating preservation, and a wax layer barrier is broken through by utilizing the nano-size effect and the interface adaptability of the saussurea nipponica polysaccharide, so that microbial proliferation can be remarkably inhibited, fat oxidation can be reduced, the water binding capacity and sensory quality can be maintained, and the shelf life can be effectively prolonged.

The present invention discloses a Lactobacillus plantarum WYL8 with anti-lipid oxidation function and its application, belonging to the field of microbial technology, and provides a Lactobacillus plantarum ( Lactiplantibacillus plantarum ) WYL8. The Lactobacillus plantarum WYL8 was deposited at the China Center for Type Culture Collection on June 21, 2024. The deposit address is Wuhan University, Wuhan, China, and the deposit number is CCTCC NO: M 20241344. And the application of Lactobacillus plantarum WYL8 in the storage of wheat germ. Applying Lactobacillus plantarum WYL8 to the storage of wheat germ inhibits the oxidative rancidity of fat, ensures the quality of wheat germ, and prevents the loss of nutritional value and economic value.

The invention discloses a method for tenderizing Tibetan mutton based on low-temperature fermentation, and belongs to the technical field of meat processing. The Tibetan mutton is subjected to low-temperature fermentation treatment by adopting a specific fermentation treatment agent, so that muscular tissues are soft, the eating quality of the Tibetan mutton is remarkably improved, meanwhile, lipid oxidation of the Tibetan mutton can be more effectively inhibited, the tenderness of the Tibetan mutton is improved, and the Tibetan mutton product can better meet the requirements of people; the method is of great significance in promoting development and utilization of Tibetan mutton.

The present invention discloses a method for one-step preparation of tuna oil Pickering emulsion, which includes: adding an aqueous solution of alkaline zein and an aqueous solution of acidic pectin, which are rapidly mixed in equal volume, as the aqueous phase into channel one, adding tuna oil as the oil phase into channel two, and adjusting the pressure and flow rate ratio through a dual-channel microfluidic device for homogenization to obtain a high-concentration tuna oil Pickering emulsion; wherein, the aqueous phase alkaline zein aqueous solution and acidic pectin aqueous solution will self-assemble to form composite nanoparticles through pH-driven, and are mixed and homogenized with tuna oil in one step through dual-channel adjustment. Compared with the traditional method for Pickering emulsion, the operation is convenient and the process is simple; the obtained tuna oil Pickering emulsion droplets are uniform and have a small particle size, and have good structural stability, anti-lipid oxidation characteristics and intestinal targeting and sustained release effects.

The present invention relates to a composition for regulating hepatic lipid metabolism, comprising Lactiplantibacillus plantarum OPT-A1 strain deposited under accession number KCTC 15555BP, a culture liquid thereof, a cultured product thereof, or a culture lysate thereof. The composition suppresses lipid accumulation and expression of genes related to lipogenesis in hepatocytes, and enhances expression of genes related to lipid oxidation. In addition, the composition inhibits accumulation of triglycerides in hepatocytes and reduces blood triglyceride levels, total cholesterol levels, and liver injury indices. Furthermore, the composition promotes rapid consumption of lipids in hepatocytes and somatic cells through enhancement of muscle mass, and thus can be advantageously used as a raw material for foods, pharmaceuticals, and the like related to alleviation and treatment of various metabolic diseases.