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213 results about "Lipid oxidation" patented technology

Transgenic soybean seeds having reduced activity of lipoxygenases

The present invention concerns a transgenic soybean plant producing seed having reduced activity of seed lipoxygenases, when compared to a soybean plant expressing wild type activity of native seed lipoxygenases, the transgenic soybean plant having a nucleic acid fragment from at least a portion of at least one soybean seed lipoxygenase gene, wherein the nucleic acid fragment is capable of suppressing expression of native seed lipoxygenases and has been introduced into the soybean plant by transformation. The present invention also concerns a transgenic soybean plant producing seed having reduced activity of seed lipoxygenases and a second native enzyme, when compared to a soybean plant expressing wild type activity of native seed lipoxygenases and the second native enzyme, the transgenic soybean plant having a first nucleic acid fragment from at least a portion of at least one soybean seed lipoxygenase gene, wherein the first nucleic acid fragment is capable of suppressing expression of said native seed lipoxygenases, and a second nucleic acid fragment from at least a portion of at least one second native enzyme gene, wherein the second nucleic acid fragment is capable of suppressing expression of the native second enzyme, wherein the first nucleic acid fragment and the second nucleic acid fragment have been introduced into the soybean plant by transformation, and wherein the second enzyme is selected from the group consisting of an enzyme of the lipid oxidation pathway, fatty acid desaturation pathway, phenylpropanoid pathway, triterpenoid pathway, and combinations thereof. Methods of suppressing wild type activity of native soybean seed lipoxygenases, alone or in combination with suppression of a second native enzyme are also embodied by the present invention.
Owner:EI DU PONT DE NEMOURS & CO

Edible film-coating freshness preservation agent used for megalobrama amblycephala and use method thereof

The invention discloses an edible film-coating freshness preservation agent used for megalobrama amblycephala, which belongs to the technical fields of the freshness preservation and the processing of aquatic products. The freshness preservation agent is a water solution prepared by dissolving the following components in water, wherein each component and the concentration thereof in the water solution are as follows: 14-16g/L of sodium alginate, 95-105g/L of glycerol and 40-60g/L of ascorbate. The invention also discloses a use method of the freshness preservation agent. After the film-coating freshness preservation agent is used for the film coating of the megalobrama amblycephala, a layer of film can be formed on the surface of the megalobrama amblycephala and acts as the functions of reducing bacterial pollution, prohibiting microbial growth, attenuating air oxidation and slowing down the lipid oxidation rate, and the effect of freshness preservation is obviously superior to that of an ordinary low-temperature preservation method. The use method of the film-coating freshness preservation agent is simple, the equipment investment is little, the operation site is small, and the film-coating freshness preservation agent can be suitable for industrial mass production.
Owner:CHINA AGRI UNIV

Meat curing agent and processing method of conditioned meat

The invention provides a meat curing agent which is capable of making the meat product high in meat tenderness and yield rate. In parts by weight, per 100 parts of the meat curing agent comprises the following ingredients: 1-3 parts of shrimp sauce, 0.001-0.01 parts of paprika red, 2-10 parts of corn starch, 0.1-0.5 part of seasonings and water accounting for all the remaining parts. The seasonings include anises, barks of Japanese cinnamon, fennels, gingers, garlic and green onions of the same parts by weight. The invention further provides a processing method of conditioned meat which is capable of making the meat product high in meat tenderness and yield rate. The beneficial effects of the processing method are as follows: the pork and the meat curing agent disclosed by the invention are mixed according to a certain ratio, then low-temperature vacuum intermittent rolling and kneading and ultrasonic treatment are performed, so that the indexes all satisfies relative national standards; the product is high in meat tenderness and yield rate (the yield rate can be as high as 152%) as well as long in shelf life; the product meets commercial sales requirements in overall sensory acceptability even after one year frozen storage at 18 DEG C below zero; moreover, the product is relatively low in lipid oxidation degree and free of oil-burning smell.
Owner:FUJIAN YAMING FOOD

Preparation of soybean anti-fatigue biological active peptide

The invention discloses preparation of a soybean anti-fatigue biological active peptide, belonging to an extraction processing technology of plant protein. The method comprises the following steps: adding water in high-denaturation soybean meal serving as a raw material to prepare a mixed solution; performing ultrasonic preprocessing on the mixed solution; adding neutral protease into the processed mixed solution to perform enzymolysis so as to obtain enzymatic hydrolysate; performing ultrafiltration treatment on the enzymatic hydrolysate by using a membrane separation technology after the enzymolysis to obtain biological active peptide with high peptide content and molecular weight of less than 5 kDa; then performing separation and purification by using anion exchange chromatography and reversed-phase high-performance liquid chromatography; and finally performing freeze drying to obtain the soybean anti-fatigue biological active peptide. The soybean anti-fatigue biological active peptide prepared by the preparation method disclosed by the invention has higher autioxidant enzyme activities and lower malondialdehyde (MDA) content after movement of a mouse; and results show that the soybean peptide has the functions of reducing cell injury caused by lipid oxidation to have an anti-fatigue effect by removing free radicals produced in the body and inhibiting the generation of lipid peroxides.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Preparation of semi-dry flavouring fish food product

The invention relates to a preparation method of a intermediate moisture seasoning fish food, which comprises the technological processes of raw ingredient fish such as preprocessing, rinsing with clear water, drying, etc. The fish bodies are soaked in mixed aqueous solution composed of edible salt and EDTA before being rinsed in the clear water; certain lso-Vc is mixed in seasoning for salting treatment before drying; the fish bodies are drying-processed by adopting the low-temperature drying process, to lead the water content of the fish bodies in the products to range from 40 to 65 percent. The mixed aqueous solution has the proportion of the edible salt and the EDTA that the edible salt is 2 to 10g and the EDTA is 0.2 to 0.5g by weight per 100ml water, and the time for soaking the fish bodies is 10 to 60 minutes. The quantity of the lso-Vc which is mixed in the seasoning is 0.2 to 0.5 percent of the weight of the salted and processed fish bodies. The preparation method is applied to solve the problems that the taste is single, the lipid oxidation is serious, the color is bad, etc. in the traditional intermediate moisture fish food; the preparation method can also enlarge the raw ingredient of the intermediate moisture fish food, and can solve the difficult problem of processing the intermediate moisture foods of high-fat fish.
Owner:ZHEJIANG GONGSHANG UNIVERSITY

Method for comprehensively packaging and refreshing chopped dry-cured ham in anti-oxidation mode

The invention relates to a method for comprehensively packaging and refreshing chopped dry-cured ham in an anti-oxidation mode and belongs to the field of food packaging. The method comprises the following steps: uniformly spraying a composite antioxidant consisting of rosemary and VE on the surface of chopped ham; coating aqueous solution of chitosan glacial acetic acid on the chopped ham; drying the ham and forming a uniform and dense protective film on the surface of the ham; and putting the ham into a small mixed deoxidant bag special for the ham which is formed by mixing and compounding a main agent consisting of reduced Fe powder and Na2S2O4 and an assistant consisting of active carbon and NaCl, and packaging the ham in a vacuum state. When the chopped ham is stored at normal temperature for six months, the TBARs and peroxide values of chopped ham muscles and subcutaneous fat are obviously reduced, the colors of fresh chopped ham muscles and fat are well kept, the oxidation degree of ham fat is remarkably reduced, the requirements on the storage of vacuum packaged chopped ham and anti-oxidation refreshment in circulation are met, and the circulation storage and shelf life of the ham are effectively prolonged.
Owner:NANJING AGRICULTURAL UNIVERSITY +1

Preparation method of Beauveria sp. extract with functions of tyrosinase inhibitor and antioxidant

The invention relates to a preparation method of Beauveria sp. extract with functiosn of a tyrosinase inhibitor and an antioxidant. The preparation method comprises the following operation steps: 1. selecting and culturing Beauveria sp. Strain; and 2. extracting and refining active components from a fermented product, wherein the refining process comprises primary refining and chromatographic refining. The Beauveria sp. and Beauveria sp. extract provided by the invention have tyrosinase inhibiting and antioxidation active effects, and the refined product has the minimum IC50 (50% inhibiting concentration) of 134.5 mu g/ml to tyrosinase and the minimum IC50 of 87.2 mu g/ml to rat liver cell lipid oxidation; the Beauveria sp. extract can be used as articles for daily use to take into effects of whitening, antioxidation, antiaging, preserving, sterilizing, anti-inflammatory and the like; because the Beauveria sp. extract is produced by microorganism fermentation, the production of the Beauveria sp. extract is not influenced by environment and natural resources, and is easy to industrialize and automatize; the process method has low production cost, simple process, stable process, and high success rate and is easy to adjust and control; and the preparation method is suitable for investment of enterprises in various scales.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Heart-nourishing yolk and walnut chicken cutlets and preparation method thereof

The invention discloses heart-nourishing yolk and walnut chicken cutlets which are prepared from the following raw materials in parts by weight: 50-60 parts of chicken breast meat, 15-16 parts of rice sprouts, 14-16 parts of walnut cream, 4-5 parts of minced chili pepper, 14-15 parts of salted duck yolk, 0.9-1 part of tuber fleeceflower stems, 1.6-2 parts of white hyacinth beans, 1.9-2 parts of clove branches and a proper amount of clove oil, thyme oil, coriandrum seed oil, sucrose ester, chitosan, a 1-2% acetate solution and lotus leaves. The chicken cutlets disclosed by the invention are savory and mellow and delicious and good in taste quality. The chitosan compound preservative film agent coating to the chicken cutlets is better in preservation effect than that of a single chitosan film liquid, so that the speed of generating hydrogen sulfide by the coated content is slowed down, lipid oxidation is slowed down and bacterial multiplication is slowed down. The storage period of the chicken cutlets is prolonged by 5-7 days compared to that in the prior art. Moreover, by adding food materials such as the tuber fleeceflower stems, the white hyacinth beans, the clove branches and the like, the heart-nourishing yolk and walnut chicken cutlets are endowed with the health-care functions of nourishing heart and soothing the nerves and warming the middle warmer and regulating qi.
Owner:全椒县福润禽业有限公司

Method for improving stewing performance and storage performance of unpolished rice

The invention relates to a method for improving the stewing performance and the storage performance of unpolished rice. The method is characterized in that the microwave and superheated steam combined treatment is adopted, the unpolished rice is properly gelatinized (the gelatinization degree is 20 to 30 percent) through controlling the water addition quantity and the water adding speed, and meanwhile, insect bacterium enzymes inside rice grains are killed or passivated for reaching the goal of improving the stewing quality and the storage performance. Through the microwave and superheated steam synergistic effect, the surface gelatinization of unpolished rice grains is very uniform, meanwhile, ova, bacteria and mycete hidden in the rice grains are effectively killed, lipoxidase and lipase in the unpolished rice are passivated, and the lipid oxidation is inhibited. Through controlling the water adding quantity and the water adding speed and adopting the low-temperature high-humidity treatment after the microwave treatment, the crackling rate of the rice grains can be effectively reduced, and the nutritional ingredient loss is reduced. The method has the advantages that the environment is protected, no pollution exists, and the stewing performance and the storage performance of products are obviously improved.
Owner:NANCHANG UNIV
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