31 results about "Lipid oxidation" patented technology

The invention discloses a preparation method of liposome for encapsulating euphausia superba oil, and belongs to the field of euphausia superba oil. Through material selection and hierarchical structure design, starch octenyl succinate, sodium alginate and pea protein are screened as composite wall materials, and the materials can achieve a synergistic effect on the molecule-interface-micro-nano scale to delay lipid oxidation, control the targeted release characteristic of active ingredients and improve the absorption efficiency of fat-soluble ingredients. Moreover, the liposome raw material of the euphausia superba oil is screened, and the liposome with low particle size and high stability is prepared.

The invention discloses a lipid oxidative metabolism characteristic analysis method and system based on REIMS and LC-MS / MS, and belongs to the technical field of lipid oxidative metabolism detection. In order to solve the detection problems of various oxidized lipids, extremely low content in a biological sample and large metabolic fluctuation, the method provided by the invention optimizes a biological sample pretreatment process, sets accurate REIMS and LC-MS / MS technical parameters, adopts two mass spectrometry technologies for combined detection and adopts an internal standard method for quantification, so that the content of the oxidized lipids in the biological sample is greatly reduced, and the content of the oxidized lipids in the biological sample is greatly reduced. And accurate qualitation and quantification of trace lipid oxidation products in a biological sample are realized. The method has the advantages that the trace lipid oxidation products in the biological sample can be accurately qualitative and quantitative, and reliable data support is provided for mining novel biomarkers.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The invention discloses a model compound-based lipid oxidation free radical specificity induction and detection method, which comprises the following steps: catalyzing a model compound (cumene / cumene hydroperoxide) or a lipid sample by using iron ions (Fe < 2 + > / Fe < 3 + >) with different valence states, and specifically inducing to generate cumene alkyl free radicals, cumene peroxide free radicals and cumene alkoxy free radicals; the method comprises the following steps: reacting a free radical trapping agent PBN with active free radicals to generate a stable PBN-free radical adduct; the adduct is separated and qualitatively identified by utilizing HPLC-TOF / MS (High Performance Liquid Chromatography-Time of Flight / Mass Spectrometry), and is accurately quantified by HPLC-UV (High The method has the advantages that three key free radicals can be specifically distinguished and quantified, the sensitivity is high, the repeatability is good, the method is suitable for free radical analysis from a model system to a complex grease sample, and a reliable analysis means is provided for research on a lipid oxidation mechanism.

The application provides a method for monitoring lipid oxidation tendency, and belongs to the technical field of fluorescent probe detection. The method utilizes the fluorescence characteristics of a BODIPY molecular probe, and through selecting a molecular probe with a specific structure, directional reaction occurs between the BODIPY molecular probe and oxygen free radicals generated by lipid oxidation at a specific pH value. The conjugated system of the BODIPY molecular probe is destroyed, the half-chlorin structure is broken, and the fluorescence color changes from red to blue-green. According to the change of the fluorescence color before and after the reaction, the visualization monitoring of the lipid oxidation tendency and spatial distribution is realized through fluorescence imaging.

The invention belongs to the field of food processing, and particularly relates to a processing method of tea-flavored fresh braised chicken capable of prolonging the shelf life and an obtained product. According to the processing method provided by the invention, under the action of the tea polyphenol, on one hand, free radicals in a lipid oxidation chain reaction are competitively removed, grease rancidity and protein oxidative degradation are inhibited, and the rising rate of putrefaction indexes such as volatile basic nitrogen and a peroxide value is reduced; on the other hand, the cell membrane integrity of escherichia coli, staphylococcus aureus and other pathogenic bacteria and molds is destroyed, and metabolism and reproduction are inhibited. After the process is optimized, the shelf life of the Texas braised chicken under the normal-temperature vacuum packaging condition is prolonged to 15-20 days compared with that of the Texas braised chicken in the traditional process, artificially synthesized preservatives do not need to be added in the whole process, and the Texas braised chicken conforms to the natural and healthy food consumption trend.

This invention discloses a method for pretreating camellia seeds using low-temperature plasma, relating to the field of bioengineering technology. The method involves discharging the entire surface of the camellia seed using DBD, JET, DF, or RF at atmospheric pressure, preferably DBD 120kV×1min or RF 3m / min at a distance of 0-3mm for 20 passes. This achieves microporousization of the seed coat and targeted disruption of the cell wall to promote mass transfer. The treatment reduces lipase and lipoxygenase activities by approximately 31.72% and 35.69% (or 29.54%), respectively, and significantly enhances the antioxidant capacity of total phenols and DPPH / ABTS / FRAP in the methanol extract (up to 1.58 times the total phenols). This method simultaneously promotes the release of active ingredients, inhibits lipid oxidation, and improves oil yield and quality under low-temperature, non-thermal conditions, making it suitable for continuous industrial applications.

A polyhydroxyalkanoate (PHA) containing monomer units of 3-hydroxybutyrate for use, by oral administration, in the prevention or treatment of an overweight or obesity condition, or of metabolic dysfunctions related to the condition. A marked effect was observed on the reduction of the body weight, both in the case of a standard diet and in the case of a diet rich in fats, a reduction that can be attributed to an increase in lipid oxidation to the detriment of the deposit of excess lipids, as confirmed by the decrease in the body weight and by analyses of the body composition. A positive effect on the metabolic dysfunctions related to an overweight or obesity condition has also been verified, in particular on dyslipidemias, such as the blood level of low-density lipoproteins (LDL cholesterol) and triglycerides, as well as an improvement in the inflammatory state.

The application discloses a CAR-T cell regulated by an ACSL4 gene and a preparation method and application thereof. The application improves the tolerance of the CAR-T cell to in-vivo lipid oxidation stress by regulating the ACSL4 related lipid metabolism process of the CAR-T cell, thereby prolonging the in-vivo persistence and treatment effect of the CAR-T cell, and provides a new technical scheme for the preparation and optimization of a cell immunotherapy product.

The invention discloses a saussurea involucrata polysaccharide, a nanoemulsion thereof, and a preparation method and application of the saussurea involucrata polysaccharide. The specific method comprises the following steps: firstly, drying and grinding saussurea involucrata peel into powder; selecting bacillus altitudinis cultured to the second generation as a fermentation strain, carrying out inoculation fermentation treatment on the powder, and filtering to obtain supernate; and adding ethanol for alcohol precipitation overnight, centrifuging, performing rotary evaporation, removing protein by a Sevage method, dialyzing, purifying, freeze-drying, and repeating the operation to obtain the product. According to the present invention, the Prunus persica pericarp polysaccharide is extracted and purified, the standardized process is established, the Prunus persica polysaccharide nano-emulsion is prepared, and the high encapsulation efficiency and the stability are achieved by optimizing the surfactant system; according to the invention, the saussurea nipponica polysaccharide and the nanoemulsion thereof are innovatively applied to shrimp coating preservation, and a wax layer barrier is broken through by utilizing the nano-size effect and the interface adaptability of the saussurea nipponica polysaccharide, so that microbial proliferation can be remarkably inhibited, fat oxidation can be reduced, the water binding capacity and sensory quality can be maintained, and the shelf life can be effectively prolonged.

The present invention relates to a composition for regulating hepatic lipid metabolism, comprising Lactiplantibacillus plantarum OPT-A1 strain deposited under accession number KCTC 15555BP, a culture liquid thereof, a cultured product thereof, or a culture lysate thereof. The composition suppresses lipid accumulation and expression of genes related to lipogenesis in hepatocytes, and enhances expression of genes related to lipid oxidation. In addition, the composition inhibits accumulation of triglycerides in hepatocytes and reduces blood triglyceride levels, total cholesterol levels, and liver injury indices. Furthermore, the composition promotes rapid consumption of lipids in hepatocytes and somatic cells through enhancement of muscle mass, and thus can be advantageously used as a raw material for foods, pharmaceuticals, and the like related to alleviation and treatment of various metabolic diseases.

This invention relates to the field of rice flour preparation, specifically to rice flour production. The method involves roasting paddy rice at 120-140°C, followed by hulling to obtain roasted rice. The roasted rice is then pulverized to obtain the rice flour. This invention employs a process of roasting, hulling, and grinding to delay the lipid oxidation and rancidity of rice flour, thus extending its shelf life. Simultaneously, the resulting raw rice flour can be enhanced by high-temperature roasting to produce a unique caramelized aroma from the paddy rice itself. This flavor significantly improves the texture and aroma distinctiveness of the rice flour. Directly roasting the paddy rice simplifies the process, saving processing time, reducing costs, and increasing production efficiency.

The present invention relates to the use of a compound for the preparation of a composition for reversing higher lipid oxidation end products (ALE) and / or higher glycosylation end products (AGE). The compounds are represented by the following formula I, wherein R1, R2, R3 and R4 are as defined in the specification. The invention also relates to a composition for reversing ALE and / or AGE comprising said compound and to a cosmetic process comprising applying an effective amount of said compound to the skin of an organism.

This invention discloses a hydrophobic active substance encapsulation formulation, its dry preparation method, and its applications, belonging to the fields of nanoencapsulation, food science, and active delivery technology. Utilizing resonant acoustic mixing technology, under completely dry conditions without organic solvents, hydrophobic active substances are mixed with food-grade protein at a specific mass ratio, and a grinding medium of a specific particle size is added. A single-step processing is performed under low-frequency, high-intensity acceleration to achieve non-covalent intercalation and assembly of the two substances. The formulation exhibits uniform particle size and high encapsulation efficiency, with the active substance completely encapsulated in an amorphous form within the protein matrix. This formulation improves the water dispersibility of the hydrophobic component and its retention rate under conditions such as in vitro digestion, heat treatment, ultraviolet irradiation, and long-term storage. In cell models, this formulation demonstrates excellent antioxidant stress and mitochondrial protection capabilities and can be directly used as a clean-label ingredient in functional baked goods, effectively masking the off-flavor of seafood protein, improving texture, and providing long-term inhibition of lipid oxidation.

The invention discloses a method for improving the fishy smell removal effect of freeze-thawed minced fillet by using a natural deep eutectic solvent, and belongs to the field of food processing. NADES is prepared from citric acid and trehalose according to the molar ratio of 2: 1 and added into mirror carp minced carp (w / w = 4%), and after freeze thawing treatment, the content of volatile compounds with the fishy smell characteristic of the minced carp, such as hexanal, heptanal and nonanal, is remarkably reduced, generation of lipid oxidation secondary products is inhibited, meanwhile, the binding capacity of protein to fishy smell substances is enhanced, and release of the substances is reduced. According to the invention, environment-friendly natural degradable materials are adopted, so that a new idea is provided for development of a novel green fishy smell removing agent while the fishy smell of the frozen and thawed surimi is effectively reduced, and a new insight is provided for flavor preservation of the surimi and more types of muscle foods.

This invention discloses a method for improving the quality of Xuanwei ham and its application, belonging to the field of food processing. The Xuanwei ham prepared by this invention through a combination of staged salting and phased temperature and humidity fermentation with mixed fermentation of two Penicillium species, *Penicillium Rubens* W10-1 and *Penicillium fusiforme* V8, effectively increases the ham's moisture content and water activity, improves its color brightness and redness, significantly reduces lipid oxidation, alters the composition of volatile flavor compounds, enhances characteristic flavors such as fruit, wine, and cream aromas, and inhibits the accumulation of undesirable compounds.

The invention relates to an additive of a diluent for preserving boar semen at 17 DEG C and an application method thereof. Myoinositol with the concentration of 1 mg / mL is added into a boar semen diluent, so that the activity and forward movement proportion of boar sperms under the preservation condition of 17 DEG C can be remarkably improved, and meanwhile, the lipid oxidation state and acrosome integrity of the boar sperms are not influenced. The method is easy and convenient to operate, low in cost, suitable for the large-scale pig farm artificial insemination technology and beneficial to prolonging the pig semen preservation time and improving the breeding efficiency.

The application discloses a method for identifying and evaluating the origin and quality of red-ear sorghum, comprising the following steps: S1, collecting a red-ear sorghum sample to be tested; S2, determining the physicochemical indexes, volatile flavor component spectrum and non-target metabolite spectrum of the sample to obtain multi-dimensional quality data; and S3, comparing and analyzing the multi-dimensional quality data obtained in the step S2 with a standard quality database of red-ear sorghum from different origins to determine the origin and / or quality characteristics of the sample to be tested. It is found that the samples from the core production area in Guizhou are characterized by high tannin content, enrichment of flavonoids and tannin secondary metabolites; the samples from the surrounding production areas in Guizhou are characterized by high starch content, high protein content and active amino acid metabolism; and the samples from the production areas outside Guizhou are characterized by high thousand-grain weight, high ester flavor substances (especially methyl hexanoate) and enrichment of lipid oxidation products.

A live tissue storage solution is formed of an aqueous culture medium including additives, inorganic salts and nutrients. The additives preferably include amino acids including but not limited to L-Arg, L-Cys, Gly, L-His, L-Ile, L-Lys, L-Leu, L-Met, L-Phe, L-Ser, L-Try, L-Try, and L-Val. The inorganic salts preferably include calcium chloride, sodium chloride, magnesium sulphate, sodium bicarbonate, sodium phosphate. The nutrients preferably include glucose, choline, folic acid, nicinamide, riboflavin, which are known to maintain cell culture. Examples include Dulbecco's Modified Eagle Medium DMEM or Minimal Essential Medium MEM with or without serum. The culture medium contains additives to reduce, inhibit, or mitigate lipid oxidation, lipotoxicy, lipid-induced responses, and lipid inflammation.

This invention discloses a kelp fucoidan, its preparation method, and its application. The kelp fucoidan includes LF3-1 and / or LF4-1. The main chain of LF3-1 is composed of alternating →3)-α-L-Fucp-(1→ and →3)-β-D-Galp-(1→). The fucose residues on the main chain of LF3-1 are sulfated at the C-4 position or connected to a first branch, which is composed of →4)-α-L-Fucp-(1→. The galactose residues on the main chain of LF3-1 are sulfated at the C-6 position or connected to a second branch, which is composed of →2,3)-β-D-Manp-(1→ and β-D-Galp-(1→). The sulfate content of LF3-1 is 26.3%, and the weight-average molecular weight of LF3-1 is 305. LF4-1 has a molecular weight of 32.7% and a weight-average molecular weight of 182.1 kDa. Its main chain consists of alternating links of →3)-α-L-Fucp-(1→ and →6)-β-D-Galp-(1→). The fucose in the main chain of LF4-1 is sulfated at C-4 or has a third branch, which is composed of →4)-α-L-Fucp-(1→. The galactose in the main chain of LF4-1 is sulfated at C-3 or has a fourth branch, which is composed of →2,4)-β-D-Manp-(1→ and β-D-Galp-(1→). LF4-1 effectively improves blood lipids and insulin resistance, promotes lipid oxidation and glucose metabolism gene expression, and thus plays a role in weight loss and lipid reduction.

The invention relates to the technical field of biology, in particular to application of beta-hydroxybutyric acid in metabolic dysfunction related fatty liver diseases. According to the application, the accelerant for promoting the intermediate metabolite beta-hydroxybutyryl coenzyme A of beta-hydroxybutyric acid is used for preparing the medicine for treating the metabolic dysfunction related fatty liver disease. According to the application, based on in-vitro experiments, it is found that the hepatocyte histone beta-hydroxybutyrylation modification level can be up-regulated by improving the concentration of beta-hydroxybutyryl coenzyme A in hepatocytes, and the expression level of PPARalpha in the hepatocytes and lipid oxidation genes regulated and controlled in the downstream of the PPARalpha is promoted; and by inhibiting the synthesis of the beta-hydroxybutyryl coenzyme A in the hepatocytes, the histone beta-hydroxybutyryl modification level in the hepatocytes can be reduced, and the accumulation of lipids in the hepatocytes can be aggravated, so that the blank between the beta-hydroxybutyryl coenzyme A and the metabolic dysfunction related fatty liver diseases in the prior art is filled.

It is known that pollutants such as pollen and PM2 5 to which we are exposed in daily life, and blue light emitted by displays of electronic devices such as personal computers and smartphones damage the skin. Pollutants and blue light generate reactive oxygen species (ROS) in cells, cause lipid oxidation, protein glycation, and the like, and cause the occurrence of wrinkles and sagging, a decrease in the transparency of the skin, and the like. In such a background, research on components that suppress ROS production has been advanced, and external preparations have been developed, but the effects thereof are not necessarily satisfactory.SOLUTION: To provide a new external preparation composition for skin or hair care suppressing ROS production and improving skin conditions by formulating an extract of a fruit of Terminalia chebula.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of food processing, and particularly relates to an astringent substance loaded emulsion and a preparation method and application thereof.The astringent substance loaded emulsion takes a solution containing astringent substances, gels and a solvent (which can contain astringent fruit juice such as roxburgh rose juice) as an inner water phase and a mixture of liquid grease, polyglycerol ricinoleate and lecithin as an outer oil phase, the emulsion is prepared by a one-step emulsification method. By regulating and controlling the gel, a stable emulsion system with high encapsulation efficiency and high loading capacity is constructed, a lipid layer formed by an outer oil phase can mask astringent taste, and the acceptability of consumers is improved. The emulsion can delay lipid oxidation and prolong the shelf life, has good smearing performance, and can be used for developing nutritional smearing sauce products with low astringent taste and high stability.

The application discloses a preparation method and application of 3,4-dihydroxyphenylacetic acid alkyl ester. Steglich esterification reaction is carried out between 3,4-dihydroxyphenylacetic acid and alkyl alcohol in an organic solvent under the action of a condensing agent and a catalyst, so that 3,4-dihydroxyphenylacetic acid alkyl ester is obtained; the condensing agent is 1-ethyl-3-(3-dimethylpropylamine) carbodiimide, the catalyst is 4-dimethylaminopyridine, and the organic solvent is N,N-dimethylformamide. The application has high reaction speed, high target product yield, and is easy to purify; the synthesized 3,4-dihydroxyphenylacetic acid alkyl ester has better effect than traditional antioxidant BHT in inhibiting O / W type emulsion lipid oxidation. Especially when the alkyl chain has a proper length, the 3,4-dihydroxyphenylacetic acid alkyl ester can be specifically positioned at a main occurrence area of lipid oxidation, namely an oil-water interface, so that the 3,4-dihydroxyphenylacetic acid alkyl ester has the best antioxidant performance in the O / W type emulsion.

The invention discloses a method for regulating and controlling the flavor of salmon oil, and belongs to the field of lipid oxidation. According to the invention, a trace amount of nano metal oxide is added, and the flavor of the salmon oil is regulated and controlled in the production and processing process of the salmon-flavor essential oil by utilizing the catalytic action of the nano metal oxide on a lipid oxidation reaction. The nanometer copper oxide is selected, the strong catalytic oxidation-reduction reaction effect of the nanometer copper oxide is utilized, lipid oxidation is promoted, the flavor of the salmon oil is regulated and controlled, energy waste caused by high temperature is avoided, and catalytic formation of the hot processing flavor of the salmon oil is facilitated. The flavored fish oil can be used for development of indoor fragrance spray, essential oil fragrance diffusion liquid, candles and the like, and a flavored commodity for creating a catering atmosphere is provided for catering enterprises.

The invention discloses a method for improving aroma quality of braised pork cooked by an induction cooker by using a soybean peptide Maillard reaction intermediate product, and belongs to the field of food processing technology and food chemistry. According to the invention, the soybean peptide Maillard reaction intermediate product is added into a braised pork gradient variable-temperature cooking system of the induction cooker, so that the reaction balance in the braised pork system is changed, the generation of Maillard reaction substances containing sulfur, nitrogen and the like in the hot working process is increased, and meanwhile, the accumulation of lipid oxidation products is inhibited; the directional enhancement of the characteristic meat flavor of the braised pork in brown sauce is realized, the control of fat flavor is realized, and the overall acceptability of the fragrance is improved.

The invention discloses a nanofiber membrane which is prepared on the basis of a triple synergic strategy and has coupled deodorization and fresh-keeping functions. The nanofiber membrane integrates triple synergic mechanisms of host-guest inclusion, physical adsorption and biological fresh-keeping. The nanofiber membrane is prepared by taking polyvinyl alcohol (PVA), beta-cyclodextrin (beta-CD) and grape seed extract (GSE) as main components and utilizing an electrostatic spinning technology. Wherein the beta-CD and the volatile component can form a host-guest inclusion compound; due to the large specific surface area of the nanofiber membrane and the communicated porous nanostructure, diffusion of volatile molecules is promoted, and rich adsorption sites are provided; gSE can inhibit microbial growth and lipid oxidation, and synergistically prevent quality deterioration. The nanofiber membrane prepared by the invention has a wide application prospect in the fields of deodorization and preservation of food.

The application belongs to the technical field of fish feed additives, and discloses a fish feed for improving the quality of fish meat after freezing and thawing and application thereof. The fish feed comprises a basic feed and theaflavins, and the mass percentage of the theaflavins in the fish feed is 0.02-0.06%. The application is to improve the anti-freezing tolerance of grass carp muscle from the source through feeding intervention, effectively inhibit the growth of ice crystals and lipid oxidation in the subsequent freezing and thawing process, significantly improve the texture properties (such as chewiness and cohesiveness) of muscle, and maintain the integrity of cell structure. The application method can be quickly popularized, has the advantages of simple operation, low cost, significant improvement effect and the like, and provides an effective way for solving the quality deterioration problem of aquatic products in the freezing, storage and transportation process.

The invention belongs to the technical field of fish feed additives, and discloses a fish feed for improving the quality of frozen and thawed fish meat and application of the fish feed. The fish feed comprises a basal feed and theabrownin, wherein the mass percentage of the theabrownin in the fish feed is 0.02-0.06%. According to the application, through feeding intervention, the anti-freezing tolerance of grass carp muscles is improved from the source, ice crystal growth and lipid oxidation can be effectively inhibited in the subsequent freezing and thawing process, the texture characteristics (such as chewiness and cohesiveness) of the muscles are remarkably improved, and the cell structure integrity is kept. The application method can be quickly popularized, has the advantages of simplicity in operation, low cost, remarkable improvement effect and the like, and provides an effective way for solving the problem of quality degradation of aquatic products in the freezing storage and transportation process.

The application belongs to the field of agricultural biotechnology, and discloses application of silencing StSDP1 gene in cultivating high-quality potatoes, specifically, the application silences StSDP1 gene through genetic engineering technology, the StSDP1 gene sequence is shown as SEQ ID NO:1, and the high quality is any one of the following a, b or c: a high linoleic acid content; b low reducing sugar content; c high aroma substance content, the application specifically down-regulates the expression of potato StSDP1 gene through RNA interference technology, and successfully creates potato germplasm resources with high linoleic acid and low glucose content. Under the condition of boiling water cooking, due to the fact that the linoleic acid content is increased by 3-4 times and the glucose content is reduced by 38%, the content of lipid oxidation polyunsaturated aldehyde in the tubers is obviously increased, so that the flavor and aroma are enhanced.