61 results about "Lipid oxidation" patented technology

The invention discloses a preparation method of liposome for encapsulating euphausia superba oil, and belongs to the field of euphausia superba oil. Through material selection and hierarchical structure design, starch octenyl succinate, sodium alginate and pea protein are screened as composite wall materials, and the materials can achieve a synergistic effect on the molecule-interface-micro-nano scale to delay lipid oxidation, control the targeted release characteristic of active ingredients and improve the absorption efficiency of fat-soluble ingredients. Moreover, the liposome raw material of the euphausia superba oil is screened, and the liposome with low particle size and high stability is prepared.

The invention provides a method for improving the oxidation resistance of phenylalanine based on N-nicotinoyl modification and application of the method. According to the method, N-nicotinoyl phenylalanine amide with antioxidant activity is obtained through chemical synthesis or enzymatic synthesis, and the antioxidant performance of N-nicotinoyl phenylalanine amide is evaluated by using an ABTS free radical scavenging method. Results show that the N-nicotinoyl phenylalanine amide can delay lipid oxidation in a food system, prolong the shelf life of the product and maintain the nutritional stability; in a cosmetic system, skin oxidative damage can be relieved, skin aging can be delayed, and skin color can be improved. The compound disclosed by the invention can show a remarkable antioxidant effect under the condition of a relatively low addition amount, so that a safe and effective antioxidant is provided for the fields of foods and cosmetics, and the compound has a good application value and a good popularization prospect.

The invention discloses a lipid oxidative metabolism characteristic analysis method and system based on REIMS and LC-MS / MS, and belongs to the technical field of lipid oxidative metabolism detection. In order to solve the detection problems of various oxidized lipids, extremely low content in a biological sample and large metabolic fluctuation, the method provided by the invention optimizes a biological sample pretreatment process, sets accurate REIMS and LC-MS / MS technical parameters, adopts two mass spectrometry technologies for combined detection and adopts an internal standard method for quantification, so that the content of the oxidized lipids in the biological sample is greatly reduced, and the content of the oxidized lipids in the biological sample is greatly reduced. And accurate qualitation and quantification of trace lipid oxidation products in a biological sample are realized. The method has the advantages that the trace lipid oxidation products in the biological sample can be accurately qualitative and quantitative, and reliable data support is provided for mining novel biomarkers.

The invention relates to an omega-3 lipid detection method based on an inorganic matrix enhanced MALDI mass spectrum. The invention discloses an omega-3 supplement identification technology of an inorganic matrix enhanced MALDI mass spectrum, which can efficiently and accurately carry out comprehensive analysis on lipid types, double bond positions and oxidation degrees in a complex lipid sample. The technology comprises the following steps: selecting an omega-3 supplement, mixing the omega-3 supplement, a cycloaddition reagent and an inorganic matrix, carrying out MALDI laser-driven online cycloaddition reaction, and analyzing the lipid double-bond isomer by mass spectrum data. The selected 25nm hydrophilic anatase titanium dioxide obviously improves the sensitivity of lipid double bond identification, the vacuum environment of the MALDI mass spectrum and shorter laser irradiation time (30s) effectively protect lipid from additional oxidation, and the original oxidation information of the lipid is retained. The method successfully identifies 30 main omega-3 lipids in four omega-3 supplements, and reveals significant differences of lipid types, omega-3 purity and lipid oxidation degrees. The method has a wide application prospect in the aspect of high-efficiency and high-throughput screening of other complex lipid information samples such as omega-3 supplements and the like.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The invention discloses a model compound-based lipid oxidation free radical specificity induction and detection method, which comprises the following steps: catalyzing a model compound (cumene / cumene hydroperoxide) or a lipid sample by using iron ions (Fe < 2 + > / Fe < 3 + >) with different valence states, and specifically inducing to generate cumene alkyl free radicals, cumene peroxide free radicals and cumene alkoxy free radicals; the method comprises the following steps: reacting a free radical trapping agent PBN with active free radicals to generate a stable PBN-free radical adduct; the adduct is separated and qualitatively identified by utilizing HPLC-TOF / MS (High Performance Liquid Chromatography-Time of Flight / Mass Spectrometry), and is accurately quantified by HPLC-UV (High The method has the advantages that three key free radicals can be specifically distinguished and quantified, the sensitivity is high, the repeatability is good, the method is suitable for free radical analysis from a model system to a complex grease sample, and a reliable analysis means is provided for research on a lipid oxidation mechanism.

The use of a cell-permeant molecular probe to assay the presence, and monitor the production, of the reactive oxygen species, superoxide, in red blood cells (RBCs) is described. The assay is performed using electron paramagnetic resonance (EPR) spectroscopy and a cell-permeant hydroxylamine spin trap. The cell-permeant molecular probe is not detected or quantifiable by EPR in its reduced form. However, upon entering the RBCs, the probe is oxidized by reacting with superoxide to yield a stable nitroxide radical that is detected and quantified using EPR. Our studies indicate that superoxide production is consistent with RBC metabolism that predicts lipid oxidation, membrane injury and poor post-transfusion performance. Therefore, measurement of reactive oxygen species can be used as an indicator of RBC quality at the time of donation and after storage.

、To provide a method for determining the smell of fish, especially the degree of smell deterioration and lipid oxidation of tilapia during the refrigeration period. 【Solution means】Steps of obtaining raw materials by killing fresh tilapia, taking slices, washing, and mixing and grinding the fish meat; steps of extracting lipids from the raw materials; steps of dividing the obtained lipids, storing them under low-temperature conditions, and measuring the degree of lipid oxidation and lipid metabolism data of tilapia at different refrigeration times by lipid metabolomics technology after the storage ends; steps of constructing a lipid metabolism network related to smell and analyzing the degree of lipid oxidation and metabolism data at different refrigeration times based on the lipid metabolism network to obtain metabolic markers corresponding to a plurality of lipids; steps of screening the obtained metabolic markers to obtain evaluation metabolic markers; steps of analyzing the degree of lipid oxidation and metabolic status of a plurality of lipids based on the evaluation metabolic markers corresponding to the plurality of lipids.

The application provides a method for monitoring lipid oxidation tendency, and belongs to the technical field of fluorescent probe detection. The method utilizes the fluorescence characteristics of a BODIPY molecular probe, and through selecting a molecular probe with a specific structure, directional reaction occurs between the BODIPY molecular probe and oxygen free radicals generated by lipid oxidation at a specific pH value. The conjugated system of the BODIPY molecular probe is destroyed, the half-chlorin structure is broken, and the fluorescence color changes from red to blue-green. According to the change of the fluorescence color before and after the reaction, the visualization monitoring of the lipid oxidation tendency and spatial distribution is realized through fluorescence imaging.

To provide a method for determining odor of fish, especially odor deterioration and lipid oxidation degree of tilapia during cold storage.SOLUTION: The method includes the steps of: killing fresh tilapia, slicing, washing, mixing and grinding the fish meat to obtain raw materials; extracting lipids from the raw materials; dividing the obtained lipids and storing them under low-temperature conditions, and after storage, measuring the lipid oxidation degree and lipid metabolic data of tilapia at different refrigeration times using lipid metabolomics technology; constructing an odor-related lipid metabolic network and analyzing the lipid oxidation degree and metabolic data at different refrigeration times based on the lipid metabolic network to obtain metabolic markers corresponding to multiple lipids; screening the obtained metabolic markers to obtain evaluation metabolic markers; and analyzing the oxidation degree and metabolic status of multiple lipids based on the evaluation metabolic markers corresponding to multiple lipids.SELECTED DRAWING: Figure 1

The invention belongs to the field of food processing, and particularly relates to a processing method of tea-flavored fresh braised chicken capable of prolonging the shelf life and an obtained product. According to the processing method provided by the invention, under the action of the tea polyphenol, on one hand, free radicals in a lipid oxidation chain reaction are competitively removed, grease rancidity and protein oxidative degradation are inhibited, and the rising rate of putrefaction indexes such as volatile basic nitrogen and a peroxide value is reduced; on the other hand, the cell membrane integrity of escherichia coli, staphylococcus aureus and other pathogenic bacteria and molds is destroyed, and metabolism and reproduction are inhibited. After the process is optimized, the shelf life of the Texas braised chicken under the normal-temperature vacuum packaging condition is prolonged to 15-20 days compared with that of the Texas braised chicken in the traditional process, artificially synthesized preservatives do not need to be added in the whole process, and the Texas braised chicken conforms to the natural and healthy food consumption trend.

The invention provides a method for inactivating buckwheat lipoxygenase activity by using superheated steam treatment, which comprises the following steps: S1, shelling treatment: milling and shelling buckwheat seeds to obtain buckwheat core powder; s2, superheated steam treatment: enabling the buckwheat core flour to be in full contact with superheated steam at the temperature of 150-190 DEG C, wherein the treatment time is 3-15 seconds; and S3, collecting and storing: collecting the buckwheat core powder treated in the step S2, cooling to room temperature, and sealing and storing the buckwheat core powder in a self-sealing bag. According to the method, through short-time high-temperature superheated steam treatment, lipid oxidation is inhibited while key deterioration enzyme systems in buckwheat are efficiently inactivated, storage rancidity is remarkably delayed, and the method has the advantages of high efficiency, quality guarantee and industrial applicability.

This invention discloses a method for pretreating camellia seeds using low-temperature plasma, relating to the field of bioengineering technology. The method involves discharging the entire surface of the camellia seed using DBD, JET, DF, or RF at atmospheric pressure, preferably DBD 120kV×1min or RF 3m / min at a distance of 0-3mm for 20 passes. This achieves microporousization of the seed coat and targeted disruption of the cell wall to promote mass transfer. The treatment reduces lipase and lipoxygenase activities by approximately 31.72% and 35.69% (or 29.54%), respectively, and significantly enhances the antioxidant capacity of total phenols and DPPH / ABTS / FRAP in the methanol extract (up to 1.58 times the total phenols). This method simultaneously promotes the release of active ingredients, inhibits lipid oxidation, and improves oil yield and quality under low-temperature, non-thermal conditions, making it suitable for continuous industrial applications.

The invention belongs to the technical field of food processing, and particularly relates to a method for delaying lipid oxidation in the frozen storage process of conditioning meat products. According to the method, the fresh meat product is firstly subjected to vacuum cooking, and then the conditioned meat product subjected to vacuum cooking is placed in a ternary dipping freezing system to be frozen for 1-2 h at the temperature of-30 DEG C to-20 DEG C. The treatment method has the beneficial effects that after the fresh meat product is treated by adopting the method in combination with conditioning and rapid freezing, the lipid oxidation rate of the conditioned meat product is remarkably reduced in the subsequent freezing storage process, meanwhile, the second-stage and third-stage structural stability of protein is higher, and the treatment method provided by the invention has the advantages that the treatment effect is good, and the treatment cost is low. The raw materials are simple and easy to obtain, the treatment cost is low, and the universality is higher.

A polyhydroxyalkanoate (PHA) containing monomer units of 3-hydroxybutyrate for use, by oral administration, in the prevention or treatment of an overweight or obesity condition, or of metabolic dysfunctions related to the condition. A marked effect was observed on the reduction of the body weight, both in the case of a standard diet and in the case of a diet rich in fats, a reduction that can be attributed to an increase in lipid oxidation to the detriment of the deposit of excess lipids, as confirmed by the decrease in the body weight and by analyses of the body composition. A positive effect on the metabolic dysfunctions related to an overweight or obesity condition has also been verified, in particular on dyslipidemias, such as the blood level of low-density lipoproteins (LDL cholesterol) and triglycerides, as well as an improvement in the inflammatory state.

The invention provides a plant antioxidant for conditioning chicken, and relates to the field of food additives, the plant antioxidant is composed of sophora flower, pomegranate peel, wild chrysanthemum flower, rosemary, cinnamon, mint, schizonepeta and sea-buckthorn; the plant antioxidant disclosed by the invention has relatively strong antioxidant capacity, especially shows a remarkable effect in the aspect of removing DPPH free radicals and ABTS < + > free radicals, in addition, the plant antioxidant not only can effectively inhibit and condition lipid oxidation in the chicken storage period and reduce the TBARS value, but also can maintain good color stability and inhibit microbial growth, and the plant antioxidant has the advantages of being safe and reliable. The sensory quality of the conditioned chicken is remarkably improved, and a safe, effective and natural solution is provided for storage and preservation of the conditioned chicken.

The invention discloses a tea polyphenol ester-phytosterol ester liposome and a preparation method of a layer-by-layer self-assembly coating, and the preparation method comprises the following steps: (1) mixing an active substance with phospholipid, glycerol, phytosterol ester and tea polyphenol palmitate in a buffer solution, and carrying out post-treatment to obtain liposome carrying the active component; (2) adding the liposome carrying the active component into the acidified gelatin solution, incubating, and centrifuging to remove free gelatin, so as to obtain gelatin-coated liposome; and dropwise adding the gelatin-coated liposome into a sodium hyaluronate solution, reacting, and centrifuging to obtain the sodium hyaluronate-gelatin coating modified layer-by-layer self-assembled liposome. The method solves the technical problems of organic solvent residue, limited phytosterol application and lipid oxidation caused by heating, greatly shortens the production time, and is suitable for large-scale industrial production. In addition, the liposome prepared by the method is proved to have good stability and antioxidant activity, and can be widely applied to the fields of foods, daily chemicals and medicines.

The application discloses a CAR-T cell regulated by an ACSL4 gene and a preparation method and application thereof. The application improves the tolerance of the CAR-T cell to in-vivo lipid oxidation stress by regulating the ACSL4 related lipid metabolism process of the CAR-T cell, thereby prolonging the in-vivo persistence and treatment effect of the CAR-T cell, and provides a new technical scheme for the preparation and optimization of a cell immunotherapy product.

The invention discloses a saussurea involucrata polysaccharide, a nanoemulsion thereof, and a preparation method and application of the saussurea involucrata polysaccharide. The specific method comprises the following steps: firstly, drying and grinding saussurea involucrata peel into powder; selecting bacillus altitudinis cultured to the second generation as a fermentation strain, carrying out inoculation fermentation treatment on the powder, and filtering to obtain supernate; and adding ethanol for alcohol precipitation overnight, centrifuging, performing rotary evaporation, removing protein by a Sevage method, dialyzing, purifying, freeze-drying, and repeating the operation to obtain the product. According to the present invention, the Prunus persica pericarp polysaccharide is extracted and purified, the standardized process is established, the Prunus persica polysaccharide nano-emulsion is prepared, and the high encapsulation efficiency and the stability are achieved by optimizing the surfactant system; according to the invention, the saussurea nipponica polysaccharide and the nanoemulsion thereof are innovatively applied to shrimp coating preservation, and a wax layer barrier is broken through by utilizing the nano-size effect and the interface adaptability of the saussurea nipponica polysaccharide, so that microbial proliferation can be remarkably inhibited, fat oxidation can be reduced, the water binding capacity and sensory quality can be maintained, and the shelf life can be effectively prolonged.

The present invention discloses a Lactobacillus plantarum WYL8 with anti-lipid oxidation function and its application, belonging to the field of microbial technology, and provides a Lactobacillus plantarum ( Lactiplantibacillus plantarum ) WYL8. The Lactobacillus plantarum WYL8 was deposited at the China Center for Type Culture Collection on June 21, 2024. The deposit address is Wuhan University, Wuhan, China, and the deposit number is CCTCC NO: M 20241344. And the application of Lactobacillus plantarum WYL8 in the storage of wheat germ. Applying Lactobacillus plantarum WYL8 to the storage of wheat germ inhibits the oxidative rancidity of fat, ensures the quality of wheat germ, and prevents the loss of nutritional value and economic value.

The invention discloses a method for tenderizing Tibetan mutton based on low-temperature fermentation, and belongs to the technical field of meat processing. The Tibetan mutton is subjected to low-temperature fermentation treatment by adopting a specific fermentation treatment agent, so that muscular tissues are soft, the eating quality of the Tibetan mutton is remarkably improved, meanwhile, lipid oxidation of the Tibetan mutton can be more effectively inhibited, the tenderness of the Tibetan mutton is improved, and the Tibetan mutton product can better meet the requirements of people; the method is of great significance in promoting development and utilization of Tibetan mutton.

The present invention discloses a method for one-step preparation of tuna oil Pickering emulsion, which includes: adding an aqueous solution of alkaline zein and an aqueous solution of acidic pectin, which are rapidly mixed in equal volume, as the aqueous phase into channel one, adding tuna oil as the oil phase into channel two, and adjusting the pressure and flow rate ratio through a dual-channel microfluidic device for homogenization to obtain a high-concentration tuna oil Pickering emulsion; wherein, the aqueous phase alkaline zein aqueous solution and acidic pectin aqueous solution will self-assemble to form composite nanoparticles through pH-driven, and are mixed and homogenized with tuna oil in one step through dual-channel adjustment. Compared with the traditional method for Pickering emulsion, the operation is convenient and the process is simple; the obtained tuna oil Pickering emulsion droplets are uniform and have a small particle size, and have good structural stability, anti-lipid oxidation characteristics and intestinal targeting and sustained release effects.

The present invention relates to a composition for regulating hepatic lipid metabolism, comprising Lactiplantibacillus plantarum OPT-A1 strain deposited under accession number KCTC 15555BP, a culture liquid thereof, a cultured product thereof, or a culture lysate thereof. The composition suppresses lipid accumulation and expression of genes related to lipogenesis in hepatocytes, and enhances expression of genes related to lipid oxidation. In addition, the composition inhibits accumulation of triglycerides in hepatocytes and reduces blood triglyceride levels, total cholesterol levels, and liver injury indices. Furthermore, the composition promotes rapid consumption of lipids in hepatocytes and somatic cells through enhancement of muscle mass, and thus can be advantageously used as a raw material for foods, pharmaceuticals, and the like related to alleviation and treatment of various metabolic diseases.

This invention relates to the field of rice flour preparation, specifically to rice flour production. The method involves roasting paddy rice at 120-140°C, followed by hulling to obtain roasted rice. The roasted rice is then pulverized to obtain the rice flour. This invention employs a process of roasting, hulling, and grinding to delay the lipid oxidation and rancidity of rice flour, thus extending its shelf life. Simultaneously, the resulting raw rice flour can be enhanced by high-temperature roasting to produce a unique caramelized aroma from the paddy rice itself. This flavor significantly improves the texture and aroma distinctiveness of the rice flour. Directly roasting the paddy rice simplifies the process, saving processing time, reducing costs, and increasing production efficiency.

The present invention relates to the use of a compound for the preparation of a composition for reversing higher lipid oxidation end products (ALE) and / or higher glycosylation end products (AGE). The compounds are represented by the following formula I, wherein R1, R2, R3 and R4 are as defined in the specification. The invention also relates to a composition for reversing ALE and / or AGE comprising said compound and to a cosmetic process comprising applying an effective amount of said compound to the skin of an organism.

The present invention relates to a method for identifying a compound that modulates lipid oxidation levels and / or atherosclerosis through the modulation of at least one of the expression, the amount, the stability and / or the biological activity of the protein FHR1 in a mammalian cell and in plasma. Further provided is a method for determining the level of lipid peroxidation and / or oxApoB100 in a sample obtained from a mammal, comprising detecting the concentration of FHR1 in the sample or a method for determining the level of ox-LDL and / or oxApoB100 in a sample obtained from a mammal, comprising detecting of binding of FHR1 in the sample to LDL and / or ApoB100. Further provided are pharmaceutical compositions comprising the compound as identified for use in medicine, in particular for use in the prevention or treatment of a disease or condition caused by increased lipid peroxidation and / or increased oxApoB100, such as, for example, selected from atherosclerosis and / or an atherosclerotic cardiovascular disease (ACVD).

The invention discloses an extraction method of theaflavin-3, 3 '-digallate and application of theaflavin-3, 3'-digallate in preparation of microcapsules and duck meat emulsion sausages, and is characterized in that a theaflavin crude product is added into an acetone solution, separation is performed through Sephadex LH-20 gel chromatographic column chromatography, the acetone solution is used as an eluent for gradient elution, an elution component is collected, and the theaflavin-3, 3 '-digallate is obtained. The preparation method comprises the following steps: dissolving theaflavin-3, 3 '-digallate in water to obtain theaflavin-3, 3'-digallate, further mixing a maltodextrin solution, an Arabic gum solution and a beta-cyclodextrin solution, adding the mixture into the theaflavin-3, 3 '-digallate solution to obtain a mixed solution, stirring, and freeze-drying to obtain the theaflavin-3, 3'-digallate microcapsule. The invention further provides application of the microcapsule in preparation of the duck meat emulsified sausage, and the microcapsule has the advantages that the lipid oxidation effect is remarkably inhibited, the flavor stability and slow release property of the duck meat emulsified sausage are improved, and the functional action time of the duck meat emulsified sausage is prolonged.

The invention discloses stable pre-emulsified grease for improving the quality of minced fillet gel, a preparation method of the stable pre-emulsified grease and a product containing the stable pre-emulsified grease, and belongs to the technical field of minced fillet product processing, the stable pre-emulsified grease for improving the quality of the minced fillet gel comprises the following raw materials: an emulsifier solution, peanut oil and epigallocatechin gallate. The preparation method comprises the following steps: combining soybean protein isolate serving as an emulsifier with peanut oil and a natural antioxidant epigallocatechin gallate (EGCG), and carrying out high-speed homogenization and high-pressure microjet treatment to prepare a pre-emulsified grease system with small particle size and high stability. The pre-emulsified grease can be uniformly dispersed in surimi, and the tissue structure and taste of surimi gel are remarkably improved. Wherein due to the introduction of EGCG, lipid oxidation is effectively inhibited, flavor deterioration is delayed, and the oxidation resistance and storage stability of the minced fillet product are improved.

This invention discloses a hydrophobic active substance encapsulation formulation, its dry preparation method, and its applications, belonging to the fields of nanoencapsulation, food science, and active delivery technology. Utilizing resonant acoustic mixing technology, under completely dry conditions without organic solvents, hydrophobic active substances are mixed with food-grade protein at a specific mass ratio, and a grinding medium of a specific particle size is added. A single-step processing is performed under low-frequency, high-intensity acceleration to achieve non-covalent intercalation and assembly of the two substances. The formulation exhibits uniform particle size and high encapsulation efficiency, with the active substance completely encapsulated in an amorphous form within the protein matrix. This formulation improves the water dispersibility of the hydrophobic component and its retention rate under conditions such as in vitro digestion, heat treatment, ultraviolet irradiation, and long-term storage. In cell models, this formulation demonstrates excellent antioxidant stress and mitochondrial protection capabilities and can be directly used as a clean-label ingredient in functional baked goods, effectively masking the off-flavor of seafood protein, improving texture, and providing long-term inhibition of lipid oxidation.

The invention discloses a method for improving the fishy smell removal effect of freeze-thawed minced fillet by using a natural deep eutectic solvent, and belongs to the field of food processing. NADES is prepared from citric acid and trehalose according to the molar ratio of 2: 1 and added into mirror carp minced carp (w / w = 4%), and after freeze thawing treatment, the content of volatile compounds with the fishy smell characteristic of the minced carp, such as hexanal, heptanal and nonanal, is remarkably reduced, generation of lipid oxidation secondary products is inhibited, meanwhile, the binding capacity of protein to fishy smell substances is enhanced, and release of the substances is reduced. According to the invention, environment-friendly natural degradable materials are adopted, so that a new idea is provided for development of a novel green fishy smell removing agent while the fishy smell of the frozen and thawed surimi is effectively reduced, and a new insight is provided for flavor preservation of the surimi and more types of muscle foods.