The invention relates to a method for separating and purifying polysaccharides from winter jujubes, which comprises the following steps: extracting primary crude polysaccharides from winter jujubes through
water extraction and
alcohol precipitation processes; carrying out deproteinization treatment on the primary crude polysaccharides through an
enzyme-
trichloroacetic acid process, and carrying out
centrifugal separation to obtain primary supernatant liquid; adding
activated carbon into the primary supernatant liquid to carry out decolorizing treatment to obtain secondary supernatant liquid; carrying out membrane
filtration on the secondary supernatant liquid to obtain secondary crude polysaccharides; carrying out primary
chromatography on the secondary crude polysaccharides on an
ion exchange column to obtain
polysaccharide DP1 crude products and
polysaccharide DP2 crude products; and respectively carrying out secondary
chromatography on the
polysaccharide DP1 crude products and the polysaccharide DP2 crude products after primary
chromatography on a gel
chromatography column to obtain polysaccharide DP1 pure products and polysaccharide DP2 pure products. According to the invention, the winter jujube polysaccharides DP1 and DP2 obtained from the winter jujube polysaccharides separated and purified from the winter jujubes have certain
antioxidant activity, the product purity is high, the
energy consumption is low, and high-quality polysaccharides lay the foundation for better development and utilization in the fields of foods, health products,
cosmetics and the like in the future.