The invention relates to a 9,10-dihydroxy-1-deoxy-taxol analogue and a preparation method thereof. The structural formula of the analogue is shown in the specification, wherein R is methylphenyl, methoxyphenyl, propylphenyl, ethylphenyl, fluorophenyl, methyl chlorophenyl, bromophenyl, furyl, thienyl or benzofuranyl. The 9,10-dihydroxy-1-deoxy-taxol analogue has exposed hydroxyls on sites 7, 9 and 10, so that the water solubility of compounds can be greatly improved; various substituents are located on a side chain on a site 13, so that the compounds are enriched, and a very important foundation is laid for the authentication of structure-activity relationship of the compounds.