Achyranthes bidentata polysaccharide polymer and its preparation method and application

A technology of sugar polymers and polymers, applied in the field of achyranthes knuckle sugar polymers and its preparation, can solve the problems of high toxicity and side effects, poor compliance, and low targeting, and achieve controllable quality, well-preserved, and simple preparation methods Effect

Inactive Publication Date: 2017-10-03
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the drugs used to prevent and treat osteoporosis are mainly bone resorption inhibitors, bone formation accelerators and mineralization drugs. These d...

Method used

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  • Achyranthes bidentata polysaccharide polymer and its preparation method and application
  • Achyranthes bidentata polysaccharide polymer and its preparation method and application
  • Achyranthes bidentata polysaccharide polymer and its preparation method and application

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Embodiment 1

[0080] The preparation method of embodiment 1 hyssop sugar polymer

[0081] The preparation method of described hyssop sugar polymer comprises the following steps:

[0082] S1 cutting: cut 10kg of dried root of Achyranthes bidentata to 1-3cm with scissors, wash it quickly with cold water, dry it, and obtain the Achyranthes bidentata segment;

[0083] S2 water extraction: add 10 times the weight of water to the segment of Achyranthes bidentata obtained in step S1, heat to 80°C for extraction, extract for 2 hours, collect the extract, dry the dregs to obtain the extract and dregs;

[0084] S3 graded alcohol precipitation:

[0085] Concentrate the extract obtained in step S2 under reduced pressure at 60°C, add ethanol until the volume concentration of ethanol is 50%, let stand at room temperature for 24 hours, centrifuge, collect the precipitate and supernatant, and obtain crude sugar polymer AB1 and supernatant 1;

[0086] After the supernatant 1 was concentrated under reduced...

Embodiment 2

[0092] Embodiment 2 Hyssop sugar polymer ABP3-2

[0093] The achyranthes polysaccharide polymer ABP3-2 is obtained by secondary purification of the achyranthes polysaccharide polymer AB3 obtained in Example 1, and the specific method is as follows:

[0094] 1) Ion-exchange column chromatography: take 100 mg of the achyranthes glycoside polymer AB3 obtained in Example 1, dissolve it in 5 mL of deionized water, and load it on a DEAE-Cellulose 52 column. Under the conditions of eluents with different salt concentrations, 2 peaks, wherein peak one is water (0M NaCl) elution part, peak two is 0.1M NaCl elution part (use phenol-sulfuric acid method to track the elution curve in the elution process, collect the sugar part respectively according to the elution curve ), after concentrating and freeze-drying the obtained eluent respectively, two kinds of sugar polymers (peak one sugar polymer, peak two sugar polymers) are obtained;

[0095] 2) Molecular sieve gel chromatography: Dissol...

Embodiment 3

[0096] Embodiment 3 hyssop sugar polymer ABW1-1

[0097] The achyranthes polysaccharide polymer ABW1-1 is obtained by secondary purification of the achyranthes polysaccharide polymer AB1 obtained in Example 1, and the specific method is as follows:

[0098] Ion-exchange column chromatography: take 100 mg of the achyranthes glycoside polymer AB1 obtained in Example 1, dissolve it in 5 mL of deionized water, and load it on a DEAE-Cellulose 52 column, and two peaks appear under the eluent conditions of different salt concentrations , wherein peak one is water (0M NaCl) elution part, peak two is 0.1M NaCl elution part (use phenol-sulfuric acid method to track elution curve in the elution process, collect sugar part respectively according to elution curve), The eluate from peak one was collected, concentrated, and freeze-dried to obtain the hyssop sugar polymer ABW1-1.

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Abstract

The invention belongs to the technical field of medicine and health care products and particularly relates to an Achyranthes bidentata polysaccharide polymer and its preparation method and application. The Achyranthes bidentata polysaccharide polymer comprises an Achyranthes bidentata polysaccharide polymer ABP3-2, an Achyranthes bidentata polysaccharide polymer ABW1-1, an Achyranthes bidentata polysaccharide polymer ABP1-2 and an Achyranthes bidentata polysaccharide polymer ABP4-3, and has molecular weight of 1000-100000Da. The preparation method has simple processes, mild reaction conditions and large-scale production feasibility. The high-purity Achyranthes bidentata polysaccharide polymer chemical structure is identified so that the structure is ensured and the structure basis is provided for study of a pharmacological activity mechanism. The pure Achyranthes bidentata polysaccharide polymer lays the foundation for Achyranthes bidentata polysaccharide polymer drugs and health products, quality control of the Achyranthes bidentata polysaccharide polymer drug and health product and deep research of the structure-activity relationship and action mechanism.

Description

technical field [0001] The invention belongs to the technical field of medicine and health care products, and in particular relates to a hyssop sugar polymer and its preparation method and application. Background technique [0002] Osteoporosis (OP) is a disease characterized by decreased bone mass, damaged bone microstructure, and decreased bone strength, resulting in increased bone fragility and susceptibility to fracture. At present, the drugs used to prevent and treat osteoporosis are mainly bone resorption inhibitors, bone formation accelerators and mineralization drugs. These drugs are all alternative treatments. During long-term use, they have large toxic and side effects, low targeting, and poor compliance. Disadvantages such as high cost. Therefore, it is necessary to find a drug with high efficiency and low toxicity to treat osteoporosis. [0003] Achyranthes bidentata Blume (Achyranthes bidentata Blume) is the dry root of Achyranthes bidentata Blume, known as on...

Claims

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Application Information

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IPC IPC(8): C08B37/00G01N21/3563G01N24/08G01N30/02G01N30/96A61P19/10
CPCC08B37/0003G01N21/3563G01N24/087G01N30/02G01N30/96
Inventor 严春艳张少杰
Owner GUANGDONG PHARMA UNIV
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