Combined technology for extracting and separating small-molecular active peptide from marine organism protein resources

Abstract

The invention discloses a combined technology for extracting and separating small-molecular active peptides from marine organism protein resources. The combined technology comprises the steps as follows: (1) an enzymatic hydrolysate is prepared through enzymolysis of marine organism protein; (2) the marine organism protein enzymatic hydrolysate is subjected to crude separation with a membrane separation technology, and small-molecular active peptides are obtained; (3) all components are obtained through sephadex gel chromatographic separation of the small-molecular active peptides; (4) all thecomponents are further separated through ion exchange chromatography and separation products of different components are obtained; (5) the separation products are purified through reverse high performance liquid chromatography, and a series of small-molecular active polypeptide compounds are finally obtained. The technical route is simple, feasible and easy to implement, a series of small-molecular active polypeptide compounds can be directly obtained from multiple marine protein resources, and research work such as structure identification, pharmacological activity research, structure-activity relationship study of small-molecular active peptide compounds with different sources and types, screening of computer simulation drugs and the like can be carried out.

Description

technical field [0001] The invention relates to the field of active peptides, in particular to a joint process for extracting, separating and purifying small-molecule active peptides from marine biological protein enzymatic hydrolyzate. Background technique [0002] Marine biologically active peptides are a general term for a class of peptides that exist in marine organisms or are produced by proteolysis and have a variety of special physiologically active peptides. There are two sources of them. One is the inherent natural active peptides in marine organisms, and the other is Bioactive peptides with various physiological functions are obtained through the hydrolysis of marine protein resources, but the content of natural active peptides in organisms is low, and extraction is difficult. Therefore, enzymatic hydrolysis products of marine protein resources are an effective way to obtain active peptides. The current research And the active peptides developed are mainly derived ...

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Problems solved by technology

[0005] At present, there have been reports of different active peptides isolated from the enzymatic hydrolyzates of different marine aquatic proteins, such as the antioxidant active peptide isolated from shellfish (CN200510000032.2; CN 201510926722.4), the anti-tumor active peptide isolated from oyster (CN201310618049.9), anti-nerve cell damage active peptide isolated from anchovy fermentation broth (CN201410173125.4), active peptide isolated from giant salamander (CN 201610314751.X), from which it can be seen that marine biological protein enzymatic hydrolyzate It contains a variety of physiologically active peptides, but the technology used in the separation process of these active peptides is relatively single or various separation techniques cannot be effectively combined, and only a single active peptide is separated, the entire extraction and separation process is cumbersome and inefficient

[0006] Peptides have different properties due to their charge, amino acid sequence, molecular weight and structure. Different separation and purification methods need to be used according to the different properties of the polypeptide. At present, a certain separation and purification method is difficult to meet people's requirements for sample purity and preparation. requirements

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