273 results about "Sephadex gels" patented technology

The invention relates to a cell surface receptor biochip based on a surface plasmon resonance biosensor, a preparation method and application thereof. The biochip is characterized in that the chip is provided with a gold surface coating at a glass substrate and the gold surface is fixed with a sephadex layer which is fixed with the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin and surface receptors are fixed by antibody capture. The preparation method includes that the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin adopts the method of antibody capture and is fixed on the surface of CM5 chip based on the surface plasmon resonance biosensor, so as to produce the protein chip of para-insulin receptors which is applicable to the mutual action between IGF-1R and IRS-1, SHC, PI3K or GRB2 and hopeful to be applied to screening cancer-fighting drugs.

The invention discloses a preparation method for extracting, separating and purifying hydroxysafflor yellow A from a Chinese medicinal material safflower at high yield and purity. The method comprises the following steps of: performing ultrasonic water extraction on the safflower medicinal material, separating by using HZ801 macroporous adsorption resin, performing LH-20 sephadex chromatographic separation, performing ultrafiltration, and performing freeze drying. The content of the prepared hydroxysafflor yellow A dry powder is 99.8 percent, and the yield of the hydroxysafflor yellow A is 62.7 percent.

The invention belongs to the medicine technical filed, in particular to a preparation method of monomeric compound ginsenoside Rg1, ginsenoside Rb1 and total arasaponin and the application in the medicine field thereof. The fresh medicinal material, the dried medicinal material and the medicinal material on the market of Panax notoginseng are taken as the raw materials; according to the polarity and the solubility property of a compound, separation and purification are carried out by adopting the solvent extraction method, the crystallization process and the chromatography and total arasaponin powder is prepared by combining the common drying means, such as decompression concentration drying, freeze drying, vacuum drying and the like; by carrying out one or more methods of recrystal, normal phase, opposite phase silica gel column chromatography, daiamid column chromatography and sephadex chromatography and the like on the powder, the ginsenoside Rg1 and the ginsenoside Rb1 monomers are prepared. The medicines which take the ginsenoside Rg1 and the ginsenoside Rb1 monomers or the total arasaponin as the active ingredients can be used for preventing and/or curing the senile dementia, the neurodegenerative diseases, the cerebrovascular disorder, various dysmnesia, the central lesion and other diseases.

The invention relates to a method for extracting high-purity hemoglobin from pig blood, and on the condition of 2 to 6 DEG C, the following operations are carried out sequentially: first, anticoagulant pig blood is used for preparing packed red blood cells; hypotonic solution is used for dissolving packed red blood cells, in which inert gas is pumped, antioxidant is added, the pH value of the solution is adjusted and protective agent and antibacterial drugs are added, and after high-speed centrifugation, crude hemoglobin solution is obtained; toluene is added into the crude hemoglobin solution which is extracted in low temperature and the hemoglobin solution in the lower layer is extracted for dialysis; after being frozen and dried the hemoglobin solution after dialysis is then dissolved into high-concentration and small-volume liquid which is then purified by a sephadex chromatography column as a sample and high-concentration hemoglobin with even molecular weight is obtained to be frozen and dried in vacuum after being filtered for removing pyrogen so as to prepare high-concentration hemoglobin dry powder with no matrix or pyrogen. The hemoglobin obtained through the method of the invention has no matrix or pyrogen, which can be further modified to produce blood substitute safely and effectively and the operation is simple; the cost is low.

The invention discloses a sewage treating agent for heavy metal pollution. The sewage treating agent is prepared from raw materials in parts by weight as follows: polyethyleneimine, carbon disulfide,sodium hydroxide, starch xanthate, bacillus mucilaginosus, concha haliotidis powder, modified zeolite powder, modified bentonite, sodium alginate, humic acid, ferric trichloride, nanometer titania, cellulose, ferrous sulfate, a photocatalyst, an efficient nickel remover, a chitosan modified polymeric flocculant, a coagulant aid, sephadex and deionized water. A preparation method of the sewage treating agent comprises steps as follows: material preparation, preparation of a mixed liquor A, preparation of a mixed liquor B and forming of the sewage treating agent. Through addition of polyethyleneimine, carbon disulfide and the coagulant aid, the sewage treating agent has a good flocculation effect on various heavy metal ions; through addition of starch xanthic acid, bacillus mucilaginosus, the concha haliotidis powder, the modified zeolite powder and the modified bentonite, the sewage treating agent has a good adsorbing effect, and the heavy metal ions in sewage are effectively removed bydouble cooperation of adsorption and flocculation.

Burdock synanthrin and its preparation method. the matter is extracted from root of burdock, white or weak yellow amorphous loose solid powder, freely soluble in water, slightly sweet, stable thermally. The left of optical rotation in water is -29.8í

The invention discloses a preparation method of silk fibroin oligopeptide for skin-care products. The preparation method comprises the following steps: (1) degumming: steeping silkworm cocoons with 0.5-0.8% Na2CO3, rinsing and drying; (2) salt removing: dissolving in boiling water through 50-60% of CaCl2, cooling and then centrifuging, and dialyzing supernatant to obtain a small-molecular silk fibroin solution; (3) double-enzymolysis: performing double-enzymolysis with alkaline protease and actinozyme, centrifuging, and dialyzing supernatant to obtain low-molecular-weight silk fibroin solution; (4) membrane separation: separating with sephadex gel chromatographic column Sephadex G-15, and collecting components to obtain a silk fibroin oligopeptide solution; and (5) drying and storage: performing ultralow-temperature freeze drying on the silk fibroin oligopeptide solution to obtain silk fibroin oligopeptide powder. The preparation method of the silk fibroin oligopeptide is simple and convenient to operate, with extraction rate of more than 93%, and is applicable to addition of raw materials into skin-care products and cosmetics.

The invention discloses a forward and reverse ABO typing and RhD blood type detecting card comprising a detecting card with six micro-columns, wherein the first three micro-columns in the six micro-columns are respectively filled with equivalent A-resistant gel, B-resistant gel and D-resistant gel, and the left three micro-columns are filled with equivalent blank gel. The forward and reverse ABO typing and RhD blood type detecting card is prepared by using the following method comprising the steps of swelling dextran gels; preparing all antibody diluents; preparing all antibody working solutions; washing the gels; and subpackaging to form the RhD blood type detecting card. According to the forward and reverse ABO typing and RhD blood type detecting card, crosslinked dextran gels with different sizes and sieving ranges are mixed and swelled by using purified water, so that the specificity is effectively ensured, meanwhile, the forward and reverse ABO typing and RhD blood type detecting card has very high sensitivity, and the ion strength of a system is effectively ensured.

The invention relates to a preparation method of a tuna blood pressure lowering peptide. The preparation method comprises steps of homogenizing tuna tissue, adding pepsin or papain of 1,000-13,000 U/g fish pulp at the pH value of 2-5, heating to 35-50 DEG C, performing enzymolysis in a water bath for 1-4 hr, heating hydrolysate with boiled water for 5-15 min for deactivating enzyme, cooling, centrifuging, and purifying with macroporous resin or dextrane gel resin. The preparation method is scientific and reasonable, and extracts blood pressure lowering peptide with the leftovers of natural tuna as raw material. The blood pressure lowering peptide prepared by hydrolysis has good effect, easily-accessible raw material and low cost, and can be used for developing health products or medical preparations. The extraction method is simple, economic and easy for operation, and can be carried out in a common biochemical laboratory.

The invention discloses a novel method for rapidly preparing hydroxytyrosol and belongs to the technical field of extraction and separation of active ingredients of natural products. According to the novel method, the olive leaf extract containing oleuropein serves as a raw material, and hydroxytyrosol is prepared by rapid hydrolysis under alkaline conditions. The method comprises the following steps: weighing a certain amount of olive leaf extract, mixing with an alkaline solution, reacting under a certain temperature and time, cooling to room temperature, adding a little amount of acid to neutralize until the pH is equal to 7, centrifuging, collecting the supernatant fluid, adding a proper amount of ethyl acetate for extracting, performing rotary evaporation to remove ethyl acetate, sequentially allowing the obtained concentrated solution to pass through macroporous resin and sephadex resin, and finally carrying out spray freeze-drying or vacuum freeze-drying to obtain high-purity hydroxytyrosol. The purity can be up to over 90 percent. The novel method is easy to operate, short in time, high in efficiency, high in quality and suitable for industrialization.

The invention discloses a preparation method and an application of an ethanediamine based porous dextrangel adsorbent. The preparation method comprises the following steps: immersing the dextrangel in de-ionized water, freezing rapidly, treating by using NaOH solution; oxidizing by epoxy chloropropane to obtain epoxy porous dextrangel; then, stirring sodium carbonate, ethanediamine, absolute ethyl alcohol and epoxy porous dextrangel at the constant temperature of 60+/-2 DEG C, carrying out backflow reaction for 2-4h, cooling, washing by using de-ionized water, carrying out suction filtering until the filtrate is neutral, washing by using a small amount of ethanol, drying in vacuum, so as to obtain the ethanediamine based porous dextrangel adsorbent. The preparation method is simple, the specific area is large, the number of quadrol groups is high, the adsorbing capacity to heavy metal is high; the dextrangel has the characteristics of light weight, low cost, good stability, degradability, environment friendliness and the like; the adsorbent provided by the invention has excellent effects when used for heavy metal treatment in wastewater and heavy metal separation and enrichment in analytical chemistry.

The invention discloses a preparation method of a xanthate macro-pore dextrangel adsorbent and an application thereof. The preparation method comprises the steps of 1) rapidly cooling dextrangel immersed by distilled water to obtain macro-pore dextrangel; 2) placing the macro-pore dextrangel into an NaOH solution with the concentration of 10-30% and reacting at a constant temperature in a range of 50-60 DEG C for 15-60 min to obtain alkalified macro-pore dextrangel; and 3) enabling the alkalified macro-pore dextrangel and carbon disulfide to react to obtain the xanthate macro-pore dextrangel adsorbent. The preparation method disclosed by the invention has the characteristics of simple preparation method, large specific surface area, great quantity of xanthate, and high adsorption capability of adsorbing heavy metal, and the dextrangel has the characteristics of light weight and low cost, good stability, degradability, environmental friendliness and the like; and the adsorbent disclosed by the invention is used for treating the heavy metal in wastewater and analyzing the separation and enriching effect of the heavy metal in chemistry.

The invention discloses a preparation method of a beta-cyclodextrin modified macroporous amino glucan adsorbent. The preparation method is characterized by comprising the steps that, by mass, 50-60% of N,N-dimethylformamide and 10-18% of beta-cyclodextrin are added and are stirred and dissolved, then 15-22% of macroporous amino glucan gel is added, the temperature is increased to 55+/-2 DEG C, the constant temperature is kept, stirring is carried out, a backflow reaction is carried out for 30-40 min, 5-12% of 1,6-hexamethylene diisocyanate is dropwise added, even stirring is carried out, a constant-temperature reaction is carried out at the temperature of 55+/-2 DEG C for 4-6 h, the sum of the percentages of all the components is 100%, the mixture is washed with ethyl alcohol after the reaction is finished and taken out to be dried in a vacuum drying box, and therefore the beta-cyclodextrin modified macroporous amino glucan adsorbent is obtained. The beta-cyclodextrin modified macroporous amino glucan adsorbent has the high adsorption capacity for bisphenol A, can be repeatedly used, and is low in cost, environmentally friendly and biodegradable.

The invention discloses a preparation method of nitrite reductase of lactobacillus casei subsp rhamnosus. The method comprises the following steps: firstly, carrying inductive culture of nitrite reductase on lactobacillus casei subsp rhamnosus 6013; adding an enzyme protective agent to extract coarse nitrite reductase when the wall is broken; after carrying out vacuum-freezing and drying, finally preparing pure nitrite reductase sequentially through anion exchange chromatography and/or sephadex chromatography. The activity of NiR extracted from lactobacillus casei subsp rhamnosus is high and the condition for degrading nitrite reductase by NiR is simple. According to the disclosed condition, the enzyme activity loss of NiR is less, and the obtained NiR is high in purity and can be used for solving problem of nitrite in three fields such as vegetable fermentation, meat products and aquatic water.

The invention discloses a scorpion venom extract extracted from East Asia scorpions. A preparation method for the scorpion venom extract comprises the following steps: scorpion venom freeze-dried powder is dissolved, centrifuged, filtered by use of 0.45 mu m and 0.22 mu m microporous membranes and freeze-dried; sephadex is added in; III-IV protein peaks of scorpion venom are collected; cation exchange chromatography is carried out; detection is carried out by use of an ultraviolet spectrophotometer; and the protein peaks are collected, so as to obtain the scorpion venom extract. Experiments prove that the scorpion venom extract has definite functions of inhibiting angiogenesis and hepatoma cell reincrease value. The invention successfully extracts effective components inhibiting angiogenesis and hepatoma cell reincrease value from the East Asia scorpions, which provides a foundation for further research and application in the future.

The invention relates to the field of separation and purification of active ingredients of a natural product, in particular to a method for preparing high-purity 1-deoxynojirimycin with a combined membrane separation and column chromatography technology. According to the method, raw materials of silkworm and white mulberry is subjected to water extraction in aid of ultrasonic waves; an ultrafiltration feeding liquid is obtained through centrifugation and micro-filtration membrane treatment; and a 1-deoxynojirimycin product with purity larger than 80% is obtained through steps of secondary ultrafiltration, nanofiltration, cation exchange resin chromatography and dextrane gel chromatography. The method has the advantages of simple process, high efficiency, safety and environment protection.

The invention discloses a preparation method of sephadex surface apigenin molecular engram sorbing material as well as the application of the sephadex surface apigenin molecular engram sorbing material to selective adsorption separation of apigenin molecules in the analysis of foods and medicine. In the method, sephadex is taken as a support, and apigenin molecular engram polymer is decorated on the surface of the sephadex. The invention is characterized in that apigenin, acrylamide, ethylene-glycol dimethyl acrylate, azodiisobutyronitrile and acylation sephadex are added in a certain proportion; and in the medium of furanidine, argon gas is used to remove oxygen, an reaction lasts for 20 to 30 h in thermostatic water bath at the temperature of 60 to 65 DEG C, and then filtering and washing are performed. Acetic acid and methanol solution with the volume fraction of 12 to 18 percent is used for Soxhlet extraction for 12 to 22 h so as to remove apigenin template molecules, and then the material is obtained through washing and drying. The invention has the advantages that a specific recognition capability to apigenin molecules is achieved, the selectivity is high, the adsorbing speed is fast, the adsorbing performance is excellent, the biological degradation is achieved, the process is simple, and regeneration capability and environmental protection are achieved.

The invention discloses a combined technology for extracting and separating small-molecular active peptides from marine organism protein resources. The combined technology comprises the steps as follows: (1) an enzymatic hydrolysate is prepared through enzymolysis of marine organism protein; (2) the marine organism protein enzymatic hydrolysate is subjected to crude separation with a membrane separation technology, and small-molecular active peptides are obtained; (3) all components are obtained through sephadex gel chromatographic separation of the small-molecular active peptides; (4) all thecomponents are further separated through ion exchange chromatography and separation products of different components are obtained; (5) the separation products are purified through reverse high performance liquid chromatography, and a series of small-molecular active polypeptide compounds are finally obtained. The technical route is simple, feasible and easy to implement, a series of small-molecular active polypeptide compounds can be directly obtained from multiple marine protein resources, and research work such as structure identification, pharmacological activity research, structure-activity relationship study of small-molecular active peptide compounds with different sources and types, screening of computer simulation drugs and the like can be carried out.

The invention discloses ulva fasciata polysaccharide and application thereof. The ulva fasciata polysaccharide is prepared by the following steps: performing an ultrasonic auxiliary water extract and alcohol precipitation process on washed ulva fasciata as a raw material, thereby obtaining crude ulva fasciata polysaccharide; decoloring and removing protein from the crude ulva fasciata polysaccharide by using a radial flow chromatographic column by tan ion exchanger A103S as a packing; separating and purifying the crude ulva fasciata polysaccharide of which the color and the protein are removed by using the radial flow chromatographic column by taking Sephadex-G100 as a packing, thereby obtaining pure ulva fasciata polysaccharide with uniform molecular weight. The method for preparing the ulva fasciata polysaccharide has the characteristics of environment protection, rapidness, high efficiency and low cost, and is applicable to on-scale production; the contents of protein and the pigment in the prepared ulva fasciata polysaccharide are both relatively low, the relatively high purity is achieved, the molecular weight distribution is concentrated, the moisture absorption and retention property is improved, and the result that of ulva fasciata polysaccharide has the shearing thickening property is found for the first time. Therefore, the ulva fasciata polysaccharide disclosed by the invention can be used as a moisture absorption and retention agent or a thickening agent.

A process for preparing the norvancomycin hydrochloride includes such steps as proportionally adding pearlite, potassium ferricyanide and zinc sulfate to the fermented liquid of norvancomycin, stirring, filter, decoloring by macroreticular resin, absorbing by glucosal gel, acidic desorption, regulating pH-6.0-7.8, filter, dissolving the filtered cake in the aqueous solution of hydrochloric acid, crystallizing in acetone, and drying. Its advantages are high quality and output rate.

The invention discloses a nano-silver/sephadex hybrid material and a preparation method as well as applications in antisepsis thereof. In the method, the sephadex is firstly prepared in NaOH aqueous solution, and no reducing agent is added at room temperature and the nano-silver/sephadex hybrid material is prepared in situ by deoxidizing silver ions through dextran. The particle size of the nano-silver in the hybrid material is about 20 to 30 nanos and the particles are uniformly distributed. The nano-silver/sephadex hybrid material has very good antibacterial action to bacillus and wide application prospect in antibacterial materials.