The invention provides a sheep
placenta antioxidant polypeptide as well as an
enzymatic hydrolysis preparation method and an application thereof. With sheep
placenta leftovers as raw materials, the sheep
placenta polypeptide, which is high in
antioxidant activity, is prepared by conducting
enzymatic hydrolysis by virtue of
papain, then conducting separation and purification by virtue of macroporous adsorption resin, an
ultrafiltration membrane,
sephadex gel
chromatography and semi-preparative RP-HPLC reversed-phase high-performance liquid
chromatography, collecting an obtained material and freeze-
drying the material, wherein the
amino acid sequence of the sheep placenta polypeptide is shown as Glu-Pro-Val-Ser-His-Phe. According to the method provided by the invention, sheep placenta
processing wastes can be effectively utilized, so as to improve an added value and increase an economic benefit, and the method has the advantages of being high in product activity, convenient and feasible in preparation process, environment-friendly and the like; with the application of the method provided by the invention, the natural polypeptide, which is high in
antioxidant activity, can be obtained, and the prepared polypeptide, replacing an artificially synthesized antioxidant, such as a functional
raw material in anti-aging
cosmetics, can be used for preparing lotions, night creams, eye creams, gels, essences, facial masks and the like.