Katsutoxin extract, preparation method and application thereof

An extraction method and extract technology are applied in the field of scorpion venom extract and its preparation, which can solve the problems of unclear active ingredients, no pharmacological research, application and research limitations, etc., and achieve inhibition of re-proliferation of liver cancer cells and inhibition of vascular endothelial growth. factor, the effect of inhibiting angiogenesis

Inactive Publication Date: 2009-02-18
INST OF BASIC MEDICINE OF SAMS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a traditional Chinese medicine, East Asian scorpion is commonly used to treat immune diseases such as cardiovascular disease and rheumatoid in clinic, but i...

Method used

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  • Katsutoxin extract, preparation method and application thereof
  • Katsutoxin extract, preparation method and application thereof
  • Katsutoxin extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: extract scorpion venom extract from East Asian pincer scorpion, the steps are as follows:

[0037] (1) Use low-temperature electrical pulses to stimulate the scorpion's cerus to obtain a liquid toxin, mix it with double distilled water, and use a volume ratio of the liquid toxin: double distilled water = 1:2, then centrifuge at 5000 rpm for 30 minutes, vacuum at low temperature After freeze-drying, the freeze-dried powder of scorpion venom is obtained.

[0038] (2) Dilute the above-mentioned scorpion venom freeze-dried powder with water to scorpion venom with a concentration of 1g / 40ml and a pH of 7.4, then centrifuge at 5000 rpm for 30 minutes, take the supernatant and centrifuge at 12000 rpm for 30 minutes, and take the supernatant .

[0039] (3) Filter the above supernatant with microporous membranes of 0.45 um and 0.22 um respectively, collect the filtrate, put it in the freezer for 24 hours and freeze at -79°C, then put the freezer into the freeze dr...

Embodiment 2

[0043] Embodiment 2: extract scorpion venom extract from East Asian pincer scorpion, the steps are as follows:

[0044] (1) Use low-temperature electrical pulses to stimulate the scorpion's cerus to obtain liquid crude poison, mix it with double distilled water, and the volume ratio of liquid crude poison: double distilled water = 1:1.5, then centrifuge at 5000 rpm for 30 minutes, vacuum at low temperature After freeze-drying, the freeze-dried powder of scorpion venom is obtained.

[0045] (2) Dilute the above scorpion venom freeze-dried powder with water to scorpion venom with a concentration of 1g / 40ml and pH7.6, then centrifuge at 5000 rpm for 30 minutes, take the supernatant and centrifuge at 11000 rpm for 30 minutes, and take the supernatant .

[0046] (3) Filter the above supernatant with a microporous filter membrane with a pore size of 0.45um and 0.22um respectively, collect the filtrate, put it in a freezer for 12 hours and freeze at -89°C, then put the freezer into ...

Embodiment 3

[0050] Embodiment 3: extract scorpion venom extract from East Asian pincer scorpion, the steps are as follows:

[0051] (1) Use low-temperature electrical pulses to stimulate the scorpion's cerus to obtain liquid toxin, mix it with double-distilled water, and use volume ratio of liquid toxin: double-distilled water = 1:22, then centrifuge at 6000 rpm for 20 minutes, vacuum at low temperature After freeze-drying, the freeze-dried powder of scorpion venom is obtained.

[0052] (2) Dilute the above scorpion venom freeze-dried powder with water to scorpion venom with a concentration of 1g / 40ml and a pH of 7.0, then centrifuge at 6000 rpm for 20 minutes, take the supernatant at 13000 rpm / centrifuge for 20 minutes, and take the supernatant .

[0053] (3) Filter the above supernatant with microporous membranes of 0.45 um and 0.22 um respectively, collect the filtrate, put it in the freezer for 36 hours and freeze at -60°C, then put the freezer into the freeze dryer to vacuum After ...

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Abstract

The invention discloses a scorpion venom extract extracted from East Asia scorpions. A preparation method for the scorpion venom extract comprises the following steps: scorpion venom freeze-dried powder is dissolved, centrifuged, filtered by use of 0.45 mu m and 0.22 mu m microporous membranes and freeze-dried; sephadex is added in; III-IV protein peaks of scorpion venom are collected; cation exchange chromatography is carried out; detection is carried out by use of an ultraviolet spectrophotometer; and the protein peaks are collected, so as to obtain the scorpion venom extract. Experiments prove that the scorpion venom extract has definite functions of inhibiting angiogenesis and hepatoma cell reincrease value. The invention successfully extracts effective components inhibiting angiogenesis and hepatoma cell reincrease value from the East Asia scorpions, which provides a foundation for further research and application in the future.

Description

technical field [0001] The invention relates to a scorpion venom extract, a preparation method thereof, and an application in anti-angiogenesis. Background technique [0002] There are many studies on scorpion venom extracts in the world, and many achievements have been made. It has been confirmed that scorpion venom extracts have definite inhibitory effects on various tumors in clinical application and basic research. Scorpion venom extracts can down-regulate bcl- 2 expression and other mechanisms to promote tumor cell apoptosis, and improve the body's immune function to fight tumors. [0003] As a traditional Chinese medicine, East Asian scorpion is commonly used to treat immune diseases such as cardiovascular disease and rheumatoid in clinic, but its active ingredients are not clear at present, and there is no more detailed pharmacological research, which makes its application and research subject to great Big restrictions. Contents of the invention [0004] Aiming at...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P35/00A61P1/16A61K35/646
Inventor 张维东贾青张月英王兆朋王朝霞王燕
Owner INST OF BASIC MEDICINE OF SAMS
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