Katsutoxin extract, preparation method and application thereof

An extraction method and extract technology are applied in the field of scorpion venom extract and its preparation, which can solve the problems of unclear active ingredients, no pharmacological research, application and research limitations, etc., and achieve inhibition of re-proliferation of liver cancer cells and inhibition of vascular endothelial growth. factor, the effect of inhibiting angiogenesis
CN101366733AInactive Publication Date: 2009-02-18INST OF BASIC MEDICINE OF SAMS
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF BASIC MEDICINE OF SAMS
Publication Date
2009-02-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a scorpion venom extract extracted from East Asia scorpions. A preparation method for the scorpion venom extract comprises the following steps: scorpion venom freeze-dried powder is dissolved, centrifuged, filtered by use of 0.45 mu m and 0.22 mu m microporous membranes and freeze-dried; sephadex is added in; III-IV protein peaks of scorpion venom are collected; cation exchange chromatography is carried out; detection is carried out by use of an ultraviolet spectrophotometer; and the protein peaks are collected, so as to obtain the scorpion venom extract. Experiments prove that the scorpion venom extract has definite functions of inhibiting angiogenesis and hepatoma cell reincrease value. The invention successfully extracts effective components inhibiting angiogenesis and hepatoma cell reincrease value from the East Asia scorpions, which provides a foundation for further research and application in the future.
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Description

technical field

[0001] The invention relates to a scorpion venom extract, a preparation method thereof, and an application in anti-angiogenesis. Background technique

[0002] There are many studies on scorpion venom extracts in the world, and many achievements have been made. It has been confirmed that scorpion venom extracts have definite inhibitory effects on various tumors in clinical application and basic research. Scorpion venom extracts can down-regulate bcl- 2 expression and other mechanisms to promote tumor cell apoptosis, and improve the body's immune function to fight tumors.

[0003] As a traditional Chinese medicine, East Asian scorpion is commonly used to treat immune diseases such as cardiovascular disease and rheumatoid in clinic, but its active ingredients are not clear at present, and there is no more detailed pharmacological research, which makes its application and research subject to great Big restrictions. Contents of the invention

[0004] Aiming at...

Claims

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