123results about How to "Good anti-tumor effect" patented technology

The invention relates to a culture medium efficiently amplifying autologous NK cells and a culture method. The culture medium internally contains interleukin 2(IL-2), interleukin 15(IL-15), interleukin 7(IL-7), interleukin 12(IL-12), tumor necrosis factor alpha (TNFalpha) and CD3-antibody, can efficiently amplify and activate NK cells so as to obtain a large quantity of highly active immune cells with majority of NK cells to be transferred back to human bodies, and has remarkable curative effect in anti-tumor, anti-virus infection and immune adjustment related diseases. The culture medium efficiently amplifying autologous NK cells comprises the culture medium for cultivating cells and added factors added into the culture medium for cultivating cells, is used for cultivating and amplifying a large quantity of autologous activated NK cells, and is characterized in that the added factor comprises interleukin 2(IL-2), interleukin 15(IL-15), interleukin 7(IL-7), interleukin 12(IL-12), tumor necrosis factor alpha (TNF alpha) and CD3-antibody.

The invention discloses a nano-particle containing docetaxel and vitamin E TPGS (d-alpha tocopheryl polyethylene glycol 1000 succinate) and a preparation method thereof. The nano-particle containing docetaxel and vitamin E TPGS comprises the following components in parts by weight: 2-10 parts of docetaxel, 1-15 parts of vitamin E TPGS, 20-65 parts of albumin, 5-50 parts of cryoprotectant, and 2-20 parts of acidic buffer salt. Relative to an existing docetaxel commercial preparation, the nano-particle containing docetaxel and vitamin E TPGS is good in drug entrapping performance, small in toxic and side effect, high in stability, good in anti-tumor curative effect and convenient to administer; the preparation method is simple, and the production cost is low.

The invention relates to corydalis and corydalis of isodon macrophylla which are extracted and separated from the isodon macrophylla and have the structural formula of ent-abietane diterpenoids, a preparation method and applications thereof. A plurality of in vitro anti-tumor activity experiments show that the compounds have significant effect of inhibiting the activity of tumor cells and can be applied in the preparation of medicaments for treating cancers and/or tumors. The invention can also provide the lead compounds for developing new medicaments for treating various common multiple cancers, thereby having important significance on developing and utilizing medicinal plant resources of China.

The invention belongs to the technical field of tea production, and particularly relates to a Tetrastigma hemsleyanum Duels et Gilg tea with high contents of Tetrastigma hemsleyanum Duels et Gilg flavone, polysaccharide and kaempferol, and a manufacturing method of the Tetrastigma hemsleyanum Duels et Gilg tea. The method comprises the following steps: (1), spreading fresh Tetrastigma hemsleyanumDuels et Gilg leaves for 10-20 minutes; (2), fixation: deactivating enzymes of the Tetrastigma hemsleyanum Duels et Gilg leaves by adopting a microwave tea fixation drier; (3), flat pressing for shaping: uniformly spreading the Tetrastigma hemsleyanum Duels et Gilg leaves subjected to fixation on a bamboo skin board, and pressing another bamboo skin board on the leaves to enable the cooled Tetrastigma hemsleyanum Duels et Gilg leaves to be shaped; and (4), drying: drying the semi-finished products processed in the step (2) by a microwave tea machine. The Tetrastigma hemsleyanum Duels et Gilg tea is natural green, is bright green in color and light green in grounds, does not contain caffeine, is rich in Tetrastigma hemsleyanum Duels et Gilg flavone, polysaccharide, kaempferol and other ingratiates, can effectively inhibit cancer cell proliferation, promotes cancer cell poptosis, has no toxic or side effects and is easy to absorb and beneficial to health. The Tetrastigma hemsleyanum Duels et Gilg leaves are tender, have less fibers, and can be eaten after being brewed.

The invention belongs to the biology medicine field, and relates to a fiber growth factor receptor (FGFR)-mediated vascular targeted liposome carrier, a preparation method thereof and an application thereof. The invention aims at solving the technical problem of proving a cationic liposome with high transfection efficiency and tumor vascular targeting. The targeted liposome is prepared by using the components with the following mixture ratios: the mixture ration of DOTAP, cholesterol and tb FGF polypeptide is: the mol ratio of the DOTAP to the cholesterol equals to 2:1-2, and the weight of the tb FGF polypeptide is 10-80 percent of the total weight of the DOTAP and the cholesterol. The liposome can be combined with the FGF receptor (FGFR) on the surface of tumor new-generated vascular cells particularly, and can realize the preparation method thereof and the application of tumor vascular targeting therapy by wrapping up various micromolecule anti-tumor drugs, therapy genes and adenoviral vectors carrying therapy genes.

The invention provides a single-domain antibody fusion protein. The single-domain antibody fusion protein comprises one or more single-domain antibodies fused with cytokines, in particular one or moreanti-PD-1 single-domain antibodies fused with IL-12 or IL-2. The invention also provides an application of the single-domain antibody fusion protein in preparing medicines for treating tumors. The fusion protein provided by the invention actively combines the advantages of small molecules, good permeability, high stability and the like of single-domain antibodies with the immunoregulation and thelike of the cytokines, and has a better anti-tumor effect.

The invention relates to a novel EGFR and CD47 targeting bispecific antibody fusion protein, and a preparation method and application thereof. The novel bispecific antibody fusion protein Bi-SP provided by the invention has a function of simultaneously binding EGFR and CD47; and at the same time of binding the EGFR, the bispecific antibody fusion protein can block or prevent the binding of the CD47 and SIRPalpha, reactivate the phagocytosis of macrophages and enhance an antitumor immune response, thereby achieving a better antitumor effect.

The invention discloses a photo-thermal chemotherapy and treatment combined microenvironment responsive drug-loading nano micelle preparation method and application. A nano micelle comprises TIID-BT,a medicine active component and an amphiphilic block polymer, and the amphiphilic block polymer refers to polyethylene glycol-polyaspartic acid. By synthesis, a near infrared TIID-BT dye and a DOX chemotherapy drug are loaded on the specific nano micelle at the same time to obtain the nano micelle which is capable of giving play to photo-thermal treatment and chemotherapy at the same time. The nano micelle enables DOX to avoid a combination effect of opsonin and a capturing effect of an MPS system to form a stable and long-acting drug delivery system with high drug loading capacity, and accordingly an antitumor effect of DOX is improved while DOX resistance of tumors is changed. In addition, due to loading of the TIID-BT dye in the nano micelle, in-vivo application effects of the TIID-BT dye are improved, combination of hoto-thermal treatment and chemotherapy is realized, and in-vivo tumor treatment effects are improved due to synergistic effects of DOX and TIID-BT.

The invention discloses an effective part of spreading hedyotis herb and a preparation method thereof. The preparation method comprises the following steps of: purifying the effective part of the spreading hedyotis herb, determining the content of total iridoid glycoside and 6-O-p-coumaroyl scandoside methyl ester in the effective part of the spreading hedyotis herb, and further defining effective anti-tumor ingredients in the effective part; the preparation method is beneficial to increase of the stability of products; meanwhile, mice anti-tumor experiments and the contrast between the effective part and a clinically commonly-used anti-tumor medicine, namely, cyclophosphamide, show that the effective part of the spreading hedyotis herb has an obvious anti-tumor effect.

The invention provides a nano-micelle drug carrier based on a vitamin E derivative, a nano-micelle drug composition as well as a preparation method and application of the nano-micelle drug composition. The nano-micelle drug carrier based on the vitamin E derivative is a nano-micelle formed by D-alpha-tocopherol polyethylene glycol 1000 succinate, D-alpha-tocopherol polyethylene glycol 2000 succinate and alpha-tocopherol succinate; and the carrier entraps a hydrophobic anti-tumor drug to obtain the nano-micelle drug composition. The D-alpha-tocopherol polyethylene glycol 1000 succinate, D-alpha-tocopherol polyethylene glycol 2000 succinate and alpha-tocopherol succinate are matched with one another and cooperate, so that the formed nano-micelle is good in stability, is small and uniform in grain size, improves drug encapsulation efficiency, has relatively good P-glycoprotein inhibiting ability, has good anti-tumor effect, is small in toxicity to normal cells and has good biocompatibility. The preparation method is simple and is wide in application prospect.

The invention relates to the field of molecular biology, in particular to a lentiviral vector of a CAR and PD1-CD28 chimeric receptor for simultaneous expression of targeted CD19 and CD20. Through theCAR and PD1-CD28 chimeric receptor which simultaneously efficiently expresses targeted CD19 and CD20, the lentiviral vector ensures that a carrier-infected T cell can not only realize targeted identification of a tumor cell which expresses CD19 and CD20 but also remove PD-L1/PD1 mediated immunosuppression, so that a stronger tumor cell killing capability is achieved. Furthermore, selection and connection sequences of key elements in the carrier are optimized, and the expression efficiency of the CAR and PD1-CD28 chimeric receptor of the targeted CD19 and CD20.

The invention provides a nanometer genetic medicine resistant to tumors and a preparation method and application thereof. The nanometer genetic medicine resistant to tumors comprises amphipathic cationic polymers, acid response functional molecules and small interference RNA, wherein the acid response functional molecules are coupled with the amphipathic cationic polymers, and the small interference RNA adheres to the surfaces of the amphipathic cationic polymers through electrostatic adsorption. The provided nanometer genetic medicine resistant to tumors is great in biocompatibility, low in toxic and side effect and high in bioavailability and has acid pH responsiveness and electric charge overturning performance; the nanometer genetic medicine resistant to tumors has a broad applicationprospect and a high application value in preparation of medicine resistant to tumors or in preparation of medicine for treating vascular normalization.

The invention discloses a high-affinity CD20-resistance monoclonal antibody and application thereof in preparing antineoplastic drugs. The antibody comprises amino acid substitutions on one or a plurality of amino acid sites of a C2B8 variable region of a heavy chain and / or a variable region of a light chain. Compared with Rituximab, the mutation antibodies have higher affinity activity, the antibody specificity is strengthened, and the lethal effect on the tumor is improved obviously.

The invention belongs to the field of medicinal preparations, and relates to a targeted nanometer delivery release system aiming at a multidrug resistant tumor and a preparation method of the targeted nanometer delivery release system. According to the system and the preparation method, the photosensitizer, namely, pyrophaeophorbide-a (PPA) is bonded to two ends of the copolymer, namely, hydroxylated-polylactic acid-polyglycolic acid (HO-PLA-PEG-PLA-OH) through a chemical bonding method, thus the product, namely, the photosensitizer-embedded polylactic acid-polyglycolic acid polymer (PPA-PLA-PEG-PLA-PPA) is obtained, then the chemotherapy medicine, namely, paclitaxel is encapsulated physically by adopting an emulsified solvent evaporation method, thus the binary-drug-loading nanometer delivery release system (PPA NP-PTX) is prepared, then F3 peptide with the specific targeting function and the cell penetrating function is modified on the surface of the system, and thus the photodynamic therapy and chemotherapy combined drug delivery nanometer delivery release system with the binary targeting property is prepared. The experiment proves that the delivery system has the strong lethal effect for drug resistant tumor cells, has the obvious in-vivo tumor targeting effect, and has the obvious treatment effect for the mice bearing the multidrug resistant tumor, and thus the combined drug delivery nanometer system has the clinical application prospect.

The invention discloses a preparation method of lysimachia capillipes saponin A. The preparation method mainly comprises the following steps: selecting a lysimachia capillipes plant raw material, extracting the lysimachia capillipes plant raw material by adopting a water or ethanol-water mixed solvent, further purifying by adopting filler such as macroporous resin to obtain a total saponin extract rich in saponin B and C, dissolving the total saponin extract in an appropriate amount of alkaline solution, reacting at 40 to 80 DEG C, neutralizing by utilizing appropriate amount of acid, and further purifying the obtained hydrolysate by adopting filler such as macroporous resin, silica gel, reverse phase silica gel and the like, to obtain a lysimachia capillipes sapoonin A extract with content more than 90 percent. The invention also discloses an application of the lysimachia capillipes saponin A. The compound of the lysimachia capillipes saponin A has a remarkable inhibition effect on 11 human tumor cell lines such as A549 non-small cell lung cancer cells, SMMC-7721 human liver cancer cells, PC3 human prostate cancer cells and the like, has a broad-spectrum anti-cancer effect, and can be used as a drug or a medicine composition to treat malignant tumors.

The invention discloses the field of tumor cellular immunotherapy and particularly relates to a tumor cell vaccine which is modified by a PD-1 neutralizing antibody with combination of a GM-CSF factor and a preparation method thereof. In the invention, tumor cellular vaccine therapy is creatively combined with antibody therapy which relieves tumor immune-suppression, so that the prepared tumor cell vaccine simultaneously secreting the PD-1 neutralizing antibody and the GM-CSF factor can not only relieve the immune-suppression status of a tumor micro-environment but also enhance an antitumor immune reaction. The tumor cell vaccine is good in antitumor treatment effect, is excellent in development value and provides a new approach of tumor cellular immunotherapy.

The invention discloses a full-nutrition composition and a preparation method thereof. The full-nutrition composition which is prepared from 15 natural foods according to a scientific formulation contains all 41 nutrients essential to human bodies and ingredients with an anti-tumor function and a tumor suppression function. The full-nutrition composition comprises full-nutrition grains, an ingredient pack and a tea pack high in EGCG (epigallocatechin gallate) content. When eating the full-nutrition composition, people can add water into the full-nutrition grains and the ingredient pack and heat in a microwave oven for 8-10min to make soft, thick and palatable porridge; after eating up the porridge for 10min, people can make tea with the tea pack and boiled water at about 95DEG C and then enjoy the tea. Since the full-nutrition composition is fully made from purely natural food materials, body resistance strengthening and health promoting can be realized in full-nutrition diet while the anti-tumor function and the tumor suppression function are realized as well.

Belonging to the field of tumor gene therapy, the present invention provides an anti-tumor RNA interference plasmid-lipidosome-heparin complex targeting the human FAK and FLK1 genes. The RNA interference expression vector can express a RNA interference sequence aimed at a target site shown by SEQ ID NO:1 on the FAK gene and a RNA interference sequence aimed at a target site shown by SEQ ID NO:2 on the FLK1 gene in the body. The FAK and PLK1 dual-gene RNA interference plasmid prepared by the present invention can effectively inhibit the growth of lung cancer cells and induce the lung cancer cells to be apoptosed. A tumor inhibition experiment conducted in the body also indicates that the FAK and PLK1 dual-gene RNA interference plasmid-lipidosome-heparin complex can notably inhibit the growth of manifold tumors and prolong the lifetime of tumor-bearing mice. The product of the anti-tumor RNA interference plasmid-lipidosome-heparin complex has obvious efficacy and can reduce dosage and improve the life quality of patients, thus having a good application prospect.

The invention provides a fucose-removed anti-HER2 antibody which is produced by cells with the FUT8 gene knocked out. The amino acid sequence of the fucose-removed anti-HER2 antibody is shown as SEQ ID NO: 1, and the nucleotide sequence is shown as SEQ ID NO: 2. Furthermore, the antibody comprises a CH2 structural domain; the amount of fucose in the CH2 structural domain is zero. The invention further provides a medicine with the fucose-removed anti-HER2 antibody serving as an active ingredient, and a reagent, a composition or a kit with the fucose-removed anti-HER2 antibody serving as an active ingredient. Compared with a fucose-containing anti-HER2 antibody, the antibody disclosed by the invention is higher in activity, and shows good tumor inhibition activity in both expression and low expression of herceptin-resistant HER2.

The invention discloses application and a preparing method of trametes robinioplila alcohol extract. The trametes robinioplila alcohol extract can be applied to preparation of anti-tumor drugs. It is found that the trametes robinioplila alcohol extract has a remarkable stomach cancer BGC823 cell and colon cancer cell HCT116 proliferation inhibiting effect, meaning that the trametes robinioplila alcohol extract has an anti-tumor function. On this basis, medicinal components of trametes robinioplila are further developed and utilized.

The invention discloses a nitrogen-substituted podophyllotoxin derivative with anti-tumor activity and a preparation method and use thereof. According to the method, 2-aminopyrimidine, 2-aminopyridine, 2-amino-3-methylpyridine, 2-amino-4-methylpyridine, 2-amino-5-methylpyridine, 2-amino-4,6-dimethyl pyridine, 3-aminopyridine, 3-amino-4-methylpyridine, 5-amino-2-methylpyridine, 3-amino-2-chloropyridine or 4-amino-2-chloropyridine is respectively introduced to an activated C-ring fourth position of a podophyllotoxin compound through nitrogen substitution reaction, so as to obtain the nitrogen-substituted podophyllotoxin derivative, represented by a formula (V) shown in the specification, with excellent anti-tumor activity. The nitrogen-substituted podophyllotoxin derivative disclosed by the invention acts on tumor cells through multiple ways and multiple target points, and the anti-tumor activity of the nitrogen-substituted podophyllotoxin derivative is remarkably improved compared with that of the podophyllotoxin compound. The compound disclosed by the invention can be used for preparing anti-tumor drugs and is clinically applied to anti-tumor treatment.

The invention discloses a traditional Chinese medicine extract with an antitumor activity. The traditional Chinese medicine extract is extracted from paliurus ramosissimus leaves. The invention also discloses a method for preparing the traditional Chinese medicine extract by alcohol extraction with an ethanol solution, and use of the traditional Chinese medicine extract in preparing a medicament for treating tumors. The traditional Chinese medicine extract has good inhibition effects on tumors.

The invention provides new application of artemisinin and a derivative thereof, namely that the artemisinin or the derivative thereof is directly used as a sensitizer for thermodynamic therapy, and isused for thermodynamic therapy of tumors; or a liposome nano-composite prepared from the artemisinin and the derivative thereof and indocyanine green is used as a sensitizer for the thermodynamic therapy, and is used for thermodynamic therapy and/or photothermal therapy of the tumors. when being used for thermodynamic therapy of the tumors, the artemisinin or the derivative thereof and the liposome nano-composite not only show excellent tumor targeting performance, but also have extremely high synergistic photothermal therapy and thermodynamic therapy anti-tumor activity, and have remarkableadvantages as an anti-cancer drug.

An application of the resorcin compounds in preparing antineoplastic medicines with high curative effect s disclosed.

The invention relates to a method for extracting and purifying antitumor polysaccharide components in sargassum muticum, which belongs to the technical field of extraction and purification methods of antitumor polysaccharide components. The method for extracting and purifying antitumor polysaccharide components in sargassum muticum comprises the following steps: (1) extracting a crude polysaccharide product: carrying out cleaning and impurity removal on sargassum muticum, drying and crushing the sargassum muticum; carrying out quenching, degreasing and room-temperature drying on the sargassum muticum; extracting the obtained product; re-extracting the obtained product, and merging extraction liquid; and carrying out rotary evaporation and graded alcohol precipitation on liquid supernatant; and (2) purifying the crude polysaccharide product: removing impurities in the crude polysaccharide product; preparing freeze-dried powder into an aqueous solution, carrying out chromatography and elution on the aqueous solution; carrying out chromatography and classification on the obtained object so as to obtain three components; and carrying out related pharmacological properties detection. According to the method for extracting and purifying antitumor polysaccharide components in sargassum muticum disclosed by the invention, polysaccharide components with a relative molecular weight in a certain range and containing a certain amount of sulfated groups are obtained, the polysaccharide components have a relatively significant anti-tumor effect, and through antitumor experiments in mice, the polysaccharide components has a regulating effect on the immune system of mice, and lay the foundation for the development of antitumor drugs without toxic and side effects.

The invention provides a medicinal composition with a synergic anti-tumor effect and application thereof. The medicinal composition is prepared from, by mole, 1-3 parts of sunitinib and 10-20 parts of dihyrdroberberine; after the sunitinib and the dihyrdroberberine are mixed in proportion, auxiliary materials are added, and the mixed materials are prepared into capsules or tablets to be used. According to the medicinal composition with the synergic anti-tumor effect and the application thereof, it is found that the dihyrdroberberine and the sunitinib have the good anti-tumor effect after being jointly applied, the anti-tumor treatment effect can be maximized through the medicine synergism of the dihyrdroberberine and the sunitinib, and the composition can be used for treatment on tumors such as lung cancer, liver cancers and colon cancers and has the very high scientific research value and the good application prospect.

The invention relates to a method for in-vitro preparation of tumor antigen-specific CD8+ T memory stem cells and application of the tumor antigen-specific CD8+ T memory stem cells in antitumor immune response. The method comprises the following steps: subjecting tumor antigen and immature dendritic cells to co-incubation so as to obtain mature dendritic cells loaded with specific tumor antigen; subjecting the mature dendritic cells and CD8+ initial T cells to co-culture so as to promote generation of tumor antigen-specific CD8+ T memory stem cells; and separating out the CD8+ T memory stem cells capable of specifically recognizing specific tumor cells. The tumor antigen-specific CD8+ T memory stem cells prepared by using the method has good antineoplastic effect, high specificity and few adverse reaction.

The invention discloses fingered citron beer and a brewing method thereof, and belongs to the field of fermented food. Barley malt and fingered citron juice are adopted as raw materials, and the fingered citron beer drink containing low-concentration alcohol is made through the technological processes of smashing, glycation, filtering, boiling, precipitation, filtering, cooling, fermentation and the like. The beer has the quality characteristics of conventional beer, and the fragrance of fingered citrons and polysaccharide, flavonoid, limonin and other matter of fingered citrons are given to the beer. The characteristic and variety of the beer are enriched, the trophic function of the beer is increased, and a new way is provided for development and utilization of fingered citron resources.

The invention discloses a cabazitaxel phospholipid composition, a preparation method thereof and an application. The cabazitaxel phospholipid composition comprises a cabazitaxel lipid complex, phospholipid and cholesterol and solves the problems that drugs are unstable, preparation steps before clinical use are complicated, an existing lipid preparation has poor in vitro and vivo stability and the like, in vivo circulation time of the drugs is prolonged, the composition has a certain targeting function, the drugs are enriched on a tumor site, toxic and side effects are reduced, and bioavailability is greatly improved.